Rh6BG026700

expansin-A23-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0001146
Physical Location & Seq
Forward (+)
4039074 .. 4076318
37245 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG026700.1

Sequence Viewer

Length: 696 bp
ATGGACCACCTTGATTGGGCTTTTCAGCATTTCAGACTTCAAAGTTTTGGTCTTGTGGTTCATTCCCGTTCTCTGGTGAATTGGTGCGAGGGGGCTTGCGGATATGGTGATCTCTTCCAACAAGGTTATGGTCTAGATACAGCAGCTCTAAGCACCACATTGTTCAACAATGGACTAACTTGCGGTGCTTGTTTCGAGATCATGTGCGTCCATGATCCACAATGGTGCATCCCTAATGCAGGCACGATCAAAATTACTGCAACAAATTTTTGTCCACCAAACTGGGCTCAAGGAGGCTGGTGCAACCCGCCATACGATCACTTCGACTTGTCCATGCCTATGTTCACAAAAATCGCGCAATACAAAGCTGGAATAGTTCCGGTCAAGTTTCGCCGAGTCCCGTGTGCTAAAACAGGAGGGGTTAAGTTTGAGCTTAAAGGAAACCCCTTCTGGATTACTGCTTTGGTTTACAATGTGGGTGGTGCCGGTGATGTTAACGCTGTCAGAATCAAAGGATCTAATTCCGGTTGGCTTCAAATGTCGCGCAATTGGGGCCAAGTTTGGCAGACCAGCGCAGACTTAGTAGGGCAAAGCTTATCATTCCAAGTCACTACCAGTGAAGACAAAACTATTGACTTTGATAATGTTGCACCAGGGAATTGGCAATTTGGTCAAACATATGAGGGGAATGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

231

Amino Acids

25.53

Weight (kDa)

6.17

Isoelectric Point (pI)

26.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 47 - 130 9.3e-21 Lytic transglycolase
Expansin_C PF01357 141 - 218 2.5e-27 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 482
AccII CGCG 2 cut(s) 356, 544
AciI CCGC 3 cut(s) 99, 183, 308
AclWI GGATC 2 cut(s) 209, 523
AcsI RAATTY 1 cut(s) 265
AfiI CCNNNNNNNGG 3 cut(s) 16, 73, 239
AgsI TTSAA 3 cut(s) 41, 166, 536
AjnI CCWGG 1 cut(s) 652
AleI CACNNNNGTG 1 cut(s) 223
AluBI AGCT 4 cut(s) 146, 368, 433, 594
AluI AGCT 4 cut(s) 146, 368, 433, 594
AlwI GGATC 2 cut(s) 209, 523
AoxI GGCC 1 cut(s) 553
ApeKI GCWGC 1 cut(s) 143
ApoI RAATTY 1 cut(s) 265
ArsI GACNNNNNNTTYG 2 cut(s) 34, 66
AspLEI GCGC 3 cut(s) 358, 546, 575
AspS9I GGNCC 2 cut(s) 4, 553
AsuHPI GGTGA 3 cut(s) 88, 119, 500
AvaII GGWCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 482
BanII GRGCYC 1 cut(s) 289
BbsI GAAGAC 1 cut(s) 627
BbvI GCAGC 1 cut(s) 155
BciT130I CCWGG 1 cut(s) 654
BfaI CTAG 1 cut(s) 134
BisI GCNGC 1 cut(s) 144
BlsI GCNGC 1 cut(s) 145
Bme1390I CCNGG 1 cut(s) 654
Bme18I GGWCC 1 cut(s) 4
BmgT120I GGNCC 2 cut(s) 4, 553
BmiI GGNNCC 2 cut(s) 484, 554
BmrFI CCNGG 1 cut(s) 654
BmrI ACTGGG 1 cut(s) 292
BmsI GCATC 1 cut(s) 237
BmuI ACTGGG 1 cut(s) 292
BpiI GAAGAC 1 cut(s) 627
BpuEI CTTGAG 1 cut(s) 273
BsaJI CCNNGG 1 cut(s) 653
BsaWI WCCGGW 2 cut(s) 379, 524
Bsc4I CCNNNNNNNGG 3 cut(s) 16, 73, 239
Bse118I RCCGGY 1 cut(s) 485
Bse1I ACTGG 2 cut(s) 287, 615
BseBI CCWGG 1 cut(s) 654
BseDI CCNNGG 1 cut(s) 653
BseGI GGATG 1 cut(s) 228
BseLI CCNNNNNNNGG 3 cut(s) 16, 73, 239
BseNI ACTGG 2 cut(s) 287, 615
BseXI GCAGC 1 cut(s) 155
Bsh1236I CGCG 2 cut(s) 356, 544
BshFI GGCC 1 cut(s) 555
BshNI GGYRCC 1 cut(s) 482
BsiSI CCGG 3 cut(s) 380, 486, 525
BslFI GGGAC 1 cut(s) 383
BslI CCNNNNNNNGG 3 cut(s) 16, 73, 239
BsmFI GGGAC 1 cut(s) 383
BsmI GAATGC 1 cut(s) 694
BsnI GGCC 1 cut(s) 555
Bsp1286I GDGCHC 1 cut(s) 289
Bsp143I GATC 6 cut(s) 109, 198, 214, 246, 316, 515
BspACI CCGC 3 cut(s) 99, 183, 308
BspANI GGCC 1 cut(s) 555
BspFNI CGCG 2 cut(s) 356, 544
BspLI GGNNCC 2 cut(s) 484, 554
BspPI GGATC 2 cut(s) 209, 523
BspT107I GGYRCC 1 cut(s) 482
BsrFI RCCGGY 1 cut(s) 485
BsrI ACTGG 2 cut(s) 287, 615
BssAI RCCGGY 1 cut(s) 485
BssECI CCNNGG 1 cut(s) 653
BssMI GATC 6 cut(s) 109, 198, 214, 246, 316, 515
Bst2UI CCWGG 1 cut(s) 654
Bst6I CTCTTC 1 cut(s) 119
BstC8I GCNNGC 2 cut(s) 97, 241
BstDEI CTNAG 2 cut(s) 149, 580
BstENI CCTNNNNNAGG 1 cut(s) 237
BstF5I GGATG 1 cut(s) 228
BstFNI CGCG 2 cut(s) 356, 544
BstHHI GCGC 3 cut(s) 358, 546, 575
BstKTI GATC 6 cut(s) 112, 201, 217, 249, 319, 518
BstMBI GATC 6 cut(s) 109, 198, 214, 246, 316, 515
BstMWI GCNNNNNNNGC 1 cut(s) 552
BstNI CCWGG 1 cut(s) 654
BstSCI CCNGG 1 cut(s) 652
BstUI CGCG 2 cut(s) 356, 544
BstV1I GCAGC 1 cut(s) 155
BstV2I GAAGAC 1 cut(s) 627
BstX2I RGATCY 1 cut(s) 515
BstXI CCANNNNNNTGG 2 cut(s) 282, 660
BstYI RGATCY 1 cut(s) 515
BsuRI GGCC 1 cut(s) 555
BtsCI GGATG 1 cut(s) 228
BtsIMutI CAGTG 1 cut(s) 622
Cac8I GCNNGC 2 cut(s) 97, 241
CfoI GCGC 3 cut(s) 358, 546, 575
Cfr10I RCCGGY 1 cut(s) 485
Cfr13I GGNCC 2 cut(s) 4, 553
CseI GACGC 1 cut(s) 196
CspCI CAANNNNNGTGG 3 cut(s) 31, 460, 495
CviAII CATG 4 cut(s) 202, 212, 334, 693
DdeI CTNAG 2 cut(s) 149, 580
DpnI GATC 6 cut(s) 111, 200, 216, 248, 318, 517
DpnII GATC 6 cut(s) 109, 198, 214, 246, 316, 515
Eam1104I CTCTTC 1 cut(s) 119
EarI CTCTTC 1 cut(s) 119
Eco24I GRGCYC 1 cut(s) 289
Eco47I GGWCC 1 cut(s) 4
EcoNI CCTNNNNNAGG 1 cut(s) 237
EcoRII CCWGG 1 cut(s) 652
EcoT22I ATGCAT 1 cut(s) 694
EcoT38I GRGCYC 1 cut(s) 289
FaeI CATG 4 cut(s) 205, 215, 337, 696
FaqI GGGAC 1 cut(s) 383
FatI CATG 4 cut(s) 201, 211, 333, 692
FauI CCCGC 1 cut(s) 315
FauNDI CATATG 1 cut(s) 679
Fnu4HI GCNGC 1 cut(s) 144
FokI GGATG 1 cut(s) 215
FriOI GRGCYC 1 cut(s) 289
Fsp4HI GCNGC 1 cut(s) 144
FspBI CTAG 1 cut(s) 134
GlaI GCGC 3 cut(s) 357, 545, 574
GluI GCNGC 1 cut(s) 144
HaeIII GGCC 1 cut(s) 555
HapII CCGG 3 cut(s) 380, 486, 525
HgaI GACGC 1 cut(s) 196
HhaI GCGC 3 cut(s) 358, 546, 575
Hin1II CATG 4 cut(s) 205, 215, 337, 696
Hin6I GCGC 3 cut(s) 356, 544, 573
HinP1I GCGC 3 cut(s) 356, 544, 573
HincII GTYRAC 1 cut(s) 496
HindII GTYRAC 1 cut(s) 496
HindIII AAGCTT 1 cut(s) 592
HinfI GANTC 2 cut(s) 396, 507
HpaI GTTAAC 1 cut(s) 496
HpaII CCGG 3 cut(s) 380, 486, 525
HphI GGTGA 3 cut(s) 88, 119, 500
Hpy166II GTNNAC 4 cut(s) 275, 345, 469, 496
Hpy188I TCNGA 2 cut(s) 35, 506
Hpy188III TCNNGA 3 cut(s) 134, 196, 451
Hpy8I GTNNAC 4 cut(s) 275, 345, 469, 496
HpyAV CCTTC 1 cut(s) 457
HpyCH4V TGCA 6 cut(s) 228, 239, 260, 303, 650, 692
HpyF10VI GCNNNNNNNGC 1 cut(s) 552
HpyF3I CTNAG 2 cut(s) 149, 580
Hsp92II CATG 4 cut(s) 205, 215, 337, 696
HspAI GCGC 3 cut(s) 356, 544, 573
KspAI GTTAAC 1 cut(s) 496
Kzo9I GATC 6 cut(s) 109, 198, 214, 246, 316, 515
Lsp1109I GCAGC 1 cut(s) 155
LweI GCATC 1 cut(s) 237
MaeI CTAG 1 cut(s) 134
MaeIII GTNAC 1 cut(s) 607
MalI GATC 6 cut(s) 111, 200, 216, 248, 318, 517
MboI GATC 6 cut(s) 109, 198, 214, 246, 316, 515
MboII GAAGA 2 cut(s) 106, 632
MfeI CAATTG 1 cut(s) 547
MflI RGATCY 1 cut(s) 515
MhlI GDGCHC 1 cut(s) 289
MluCI AATT 7 cut(s) 79, 252, 265, 520, 547, 658, 665
MlyI GAGTC 1 cut(s) 405
MmeI TCCRAC 1 cut(s) 142
MnlI CCTC 4 cut(s) 82, 287, 410, 676
Mph1103I ATGCAT 1 cut(s) 694
MseI TTAA 3 cut(s) 423, 435, 495
MslI CAYNNNNRTG 2 cut(s) 223, 338
MspI CCGG 3 cut(s) 380, 486, 525
MspR9I CCNGG 1 cut(s) 654
MunI CAATTG 1 cut(s) 547
Mva1269I GAATGC 1 cut(s) 694
MvaI CCWGG 1 cut(s) 654
MvnI CGCG 2 cut(s) 356, 544
MwoI GCNNNNNNNGC 1 cut(s) 552
NdeI CATATG 1 cut(s) 679
NdeII GATC 6 cut(s) 109, 198, 214, 246, 316, 515
NlaIII CATG 4 cut(s) 205, 215, 337, 696
NlaIV GGNNCC 2 cut(s) 484, 554
NmeAIII GCCGAG 1 cut(s) 419
NmuCI GTSAC 1 cut(s) 607
NsiI ATGCAT 1 cut(s) 694
OliI CACNNNNGTG 1 cut(s) 223
PcsI WCGNNNNNNNCGW 1 cut(s) 321
PctI GAATGC 1 cut(s) 694
PfeI GAWTC 1 cut(s) 507
PkrI GCNGC 1 cut(s) 145
PleI GAGTC 1 cut(s) 404
PpsI GAGTC 1 cut(s) 404
Psp6I CCWGG 1 cut(s) 652
PspGI CCWGG 1 cut(s) 652
PspN4I GGNNCC 2 cut(s) 484, 554
PspPI GGNCC 2 cut(s) 4, 553
PsuI RGATCY 1 cut(s) 515
RseI CAYNNNNRTG 2 cut(s) 223, 338
SaqAI TTAA 3 cut(s) 423, 435, 495
SatI GCNGC 1 cut(s) 144
Sau3AI GATC 6 cut(s) 109, 198, 214, 246, 316, 515
Sau96I GGNCC 2 cut(s) 4, 553
SchI GAGTC 1 cut(s) 405
ScrFI CCNGG 1 cut(s) 654
SduI GDGCHC 1 cut(s) 289
SetI ASST 6 cut(s) 12, 127, 148, 370, 435, 596
SfaNI GCATC 1 cut(s) 237
SinI GGWCC 1 cut(s) 4
SmiMI CAYNNNNRTG 2 cut(s) 223, 338
SmlI CTYRAG 1 cut(s) 288
SmoI CTYRAG 1 cut(s) 288
Sse9I AATT 7 cut(s) 79, 252, 265, 520, 547, 658, 665
SsiI CCGC 3 cut(s) 99, 183, 308
SspMI CTAG 1 cut(s) 134
StyD4I CCNGG 1 cut(s) 652
TaqI TCGA 2 cut(s) 195, 324
TasI AATT 7 cut(s) 79, 252, 265, 520, 547, 658, 665
TfiI GAWTC 1 cut(s) 507
Tru1I TTAA 3 cut(s) 423, 435, 495
Tru9I TTAA 3 cut(s) 423, 435, 495
TscAI CASTG 1 cut(s) 622
TseFI GTSAC 1 cut(s) 607
TseI GCWGC 1 cut(s) 143
Tsp45I GTSAC 1 cut(s) 607
TspDTI ATGAA 1 cut(s) 50
TspRI CASTG 1 cut(s) 622
VpaK11BI GGWCC 1 cut(s) 4
XagI CCTNNNNNAGG 1 cut(s) 237
XapI RAATTY 1 cut(s) 265
XbaI TCTAGA 1 cut(s) 133
XcmI CCANNNNNNNNNTGG 1 cut(s) 125
XspI CTAG 1 cut(s) 134
Zsp2I ATGCAT 1 cut(s) 694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.