pycom17g05780

expansin-A23-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0001146
Physical Location & Seq
Forward (+)
4086941 .. 4087898
958 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g05780.2

Sequence Viewer

Length: 855 bp
ATGGCTAAGCTTCCAAAGAACTTGTTCACATCTGCAGTGTTGATCACTCTTTTGGTTCAAGCCATGGGTTACATAATCCCTACCGATATGAGAGCCGGTCACGGCTATATAATCCCTACCGGTGGAAGCATCGGTAACGGCTATGTAAACCCAACAGGAGGAAGAGACGGCAATGTTTGGCAAGATGCACATGCAACGTTTTACGGTGACATGAACGGAGGAGATACCATGCAGGGGGCTTGTGGATACGGCAATCTCCATGAACAAGGCTATGGCCTCGAGACAGCAGCCCTAAGTACGGCCCTCTTCAACAATGGCGGTAGTTGCGGTGCTTGTTTCCAGCTCATGTGCGTCAATGATCCCCAGTGGTGCAACCCTCACGTTAGAGCCATCACAATTACCGCAACCAACTTTTGTCCGCCAAATTGGGTTCCGGCTCCTGATCACTGGTGCAATCCACCCCAAAAGCACTTCGACCTGTCCATGCCTATGTTCGCAAAGCTCGCACAGACAAAAGCTGGTATCATACCAGTTAAGTACCGCAGAGTCTCGTGCCAGAAGAGAGGAGGGGTTAAGTTCGAGCTCAAGGGCAACCCCTGGTGGCTTACCGCGACAGTGTTCAACGTCGGTGGTGCTGGCGATGTTACAAACGTCAGACTCAAAGGATCTAGCAGGAATTGGATTCAAATGTCAAGAAATTGGGGGCAAGTTTGGGTGGCGACCGGAAACCAACTTGTAGGGCAAAGCTTGTCATTCCTAGTGACTACGAGTGATGGGAAAACAATAGAGTTGGATAATGTCGCACCAGCAAATTGGCAGTTTGGACAAACCTTTGAGGGGAGAATAAATTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

285

Amino Acids

30.87

Weight (kDa)

8.78

Isoelectric Point (pI)

21.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 611
AciI CCGC 6 cut(s) 318, 327, 402, 419, 541, 609
AclI AACGTT 1 cut(s) 197
AclWI GGATC 2 cut(s) 353, 673
AcsI RAATTY 1 cut(s) 847
AfaI GTAC 2 cut(s) 298, 539
AfiI CCNNNNNNNGG 5 cut(s) 122, 158, 234, 298, 837
AgeI ACCGGT 1 cut(s) 119
AgsI TTSAA 4 cut(s) 59, 310, 622, 686
AjnI CCWGG 1 cut(s) 596
AluBI AGCT 6 cut(s) 10, 343, 502, 518, 583, 747
AluI AGCT 6 cut(s) 10, 343, 502, 518, 583, 747
Alw21I GWGCWC 1 cut(s) 585
Alw26I GTCTC 3 cut(s) 159, 275, 553
AlwI GGATC 2 cut(s) 353, 673
Ama87I CYCGRG 1 cut(s) 278
AoxI GGCC 2 cut(s) 274, 300
ApeKI GCWGC 1 cut(s) 287
ApoI RAATTY 1 cut(s) 847
AsiGI ACCGGT 1 cut(s) 119
Asp700I GAANNNNTTC 1 cut(s) 23
AspS9I GGNCC 1 cut(s) 301
AsuHPI GGTGA 1 cut(s) 218
AvaI CYCGRG 1 cut(s) 278
BanII GRGCYC 1 cut(s) 585
BauI CACGAG 1 cut(s) 550
Bbv12I GWGCWC 1 cut(s) 585
BbvI GCAGC 1 cut(s) 299
BccI CCATC 2 cut(s) 398, 767
BceAI ACGGC 5 cut(s) 118, 154, 184, 265, 315
BciT130I CCWGG 1 cut(s) 598
BciVI GTATCC 1 cut(s) 239
BclI TGATCA 2 cut(s) 42, 442
BcoDI GTCTC 3 cut(s) 159, 275, 553
BfaI CTAG 3 cut(s) 669, 758, 853
BfmI CTRYAG 1 cut(s) 33
BfuI GTATCC 1 cut(s) 239
BisI GCNGC 1 cut(s) 288
BlpI GCTNAGC 1 cut(s) 6
BlsI GCNGC 1 cut(s) 289
Bme1390I CCNGG 1 cut(s) 598
BmeT110I CYCGRG 1 cut(s) 278
BmgT120I GGNCC 1 cut(s) 301
BmiI GGNNCC 2 cut(s) 432, 438
BmrFI CCNGG 1 cut(s) 598
BmrI ACTGGG 1 cut(s) 358
BmsI GCATC 2 cut(s) 138, 175
BmuI ACTGGG 1 cut(s) 358
Bpu1102I GCTNAGC 1 cut(s) 6
BpuEI CTTGAG 1 cut(s) 569
BsaJI CCNNGG 2 cut(s) 63, 596
BsaWI WCCGGW 2 cut(s) 119, 722
Bsc4I CCNNNNNNNGG 5 cut(s) 122, 158, 234, 298, 837
Bse118I RCCGGY 2 cut(s) 95, 119
Bse1I ACTGG 3 cut(s) 364, 452, 530
Bse3DI GCAATG 1 cut(s) 178
BseBI CCWGG 1 cut(s) 598
BseDI CCNNGG 2 cut(s) 63, 596
BseLI CCNNNNNNNGG 5 cut(s) 122, 158, 234, 298, 837
BseMI GCAATG 1 cut(s) 178
BseNI ACTGG 3 cut(s) 364, 452, 530
BseRI GAGGAG 2 cut(s) 234, 579
BseXI GCAGC 1 cut(s) 299
Bsh1236I CGCG 1 cut(s) 611
Bsh1285I CGRYCG 1 cut(s) 723
BshFI GGCC 2 cut(s) 276, 302
BshTI ACCGGT 1 cut(s) 119
BsiEI CGRYCG 1 cut(s) 723
BsiHKAI GWGCWC 1 cut(s) 585
BsiHKCI CYCGRG 1 cut(s) 278
BsiSI CCGG 4 cut(s) 96, 120, 434, 723
BslI CCNNNNNNNGG 5 cut(s) 122, 158, 234, 298, 837
BsmAI GTCTC 3 cut(s) 159, 275, 553
BsmBI CGTCTC 1 cut(s) 159
BsnI GGCC 2 cut(s) 276, 302
BsoBI CYCGRG 1 cut(s) 278
Bsp1286I GDGCHC 1 cut(s) 585
Bsp143I GATC 4 cut(s) 42, 358, 442, 665
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 1 cut(s) 63
BspACI CCGC 6 cut(s) 318, 327, 402, 419, 541, 609
BspANI GGCC 2 cut(s) 276, 302
BspFNI CGCG 1 cut(s) 611
BspLI GGNNCC 2 cut(s) 432, 438
BspMAI CTGCAG 1 cut(s) 37
BspPI GGATC 2 cut(s) 353, 673
BsrDI GCAATG 1 cut(s) 178
BsrFI RCCGGY 2 cut(s) 95, 119
BsrI ACTGG 3 cut(s) 364, 452, 530
BssAI RCCGGY 2 cut(s) 95, 119
BssECI CCNNGG 2 cut(s) 63, 596
BssMI GATC 4 cut(s) 42, 358, 442, 665
BssSI CACGAG 1 cut(s) 550
BssT1I CCWWGG 1 cut(s) 63
Bst2BI CACGAG 1 cut(s) 550
Bst2UI CCWGG 1 cut(s) 598
Bst4CI ACNGT 2 cut(s) 206, 616
Bst6I CTCTTC 3 cut(s) 157, 311, 554
BstC8I GCNNGC 2 cut(s) 504, 637
BstDEI CTNAG 2 cut(s) 6, 293
BstDSI CCRYGG 1 cut(s) 63
BstFNI CGCG 1 cut(s) 611
BstKTI GATC 4 cut(s) 45, 361, 445, 668
BstMAI GTCTC 3 cut(s) 159, 275, 553
BstMBI GATC 4 cut(s) 42, 358, 442, 665
BstMCI CGRYCG 1 cut(s) 723
BstMWI GCNNNNNNNGC 2 cut(s) 324, 503
BstNI CCWGG 1 cut(s) 598
BstNSI RCATGY 1 cut(s) 194
BstSCI CCNGG 1 cut(s) 596
BstSFI CTRYAG 1 cut(s) 33
BstUI CGCG 1 cut(s) 611
BstV1I GCAGC 1 cut(s) 299
BstX2I RGATCY 1 cut(s) 665
BstXI CCANNNNNNTGG 1 cut(s) 813
BstYI RGATCY 1 cut(s) 665
BsuI GTATCC 1 cut(s) 239
BsuRI GGCC 2 cut(s) 276, 302
BtgI CCRYGG 1 cut(s) 63
BtgZI GCGATG 1 cut(s) 654
BtsI GCAGTG 1 cut(s) 42
BtsIMutI CAGTG 4 cut(s) 42, 371, 445, 621
Cac8I GCNNGC 2 cut(s) 504, 637
Cfr10I RCCGGY 2 cut(s) 95, 119
Cfr13I GGNCC 1 cut(s) 301
CseI GACGC 1 cut(s) 340
Csp6I GTAC 2 cut(s) 297, 538
CspAI ACCGGT 1 cut(s) 119
CspCI CAANNNNNGTGG 2 cut(s) 610, 645
CviAII CATG 7 cut(s) 64, 191, 211, 229, 260, 346, 484
CviQI GTAC 2 cut(s) 297, 538
DdeI CTNAG 2 cut(s) 6, 293
DpnI GATC 4 cut(s) 44, 360, 444, 667
DpnII GATC 4 cut(s) 42, 358, 442, 665
Eam1104I CTCTTC 3 cut(s) 157, 311, 554
EarI CTCTTC 3 cut(s) 157, 311, 554
EciI GGCGGA 1 cut(s) 408
Ecl136II GAGCTC 1 cut(s) 583
Eco130I CCWWGG 1 cut(s) 63
Eco24I GRGCYC 1 cut(s) 585
Eco53kI GAGCTC 1 cut(s) 583
Eco88I CYCGRG 1 cut(s) 278
EcoICRI GAGCTC 1 cut(s) 583
EcoRII CCWGG 1 cut(s) 596
EcoT14I CCWWGG 1 cut(s) 63
EcoT38I GRGCYC 1 cut(s) 585
ErhI CCWWGG 1 cut(s) 63
Esp3I CGTCTC 1 cut(s) 159
FaeI CATG 7 cut(s) 67, 194, 214, 232, 263, 349, 487
FatI CATG 7 cut(s) 63, 190, 210, 228, 259, 345, 483
FbaI TGATCA 2 cut(s) 42, 442
Fnu4HI GCNGC 1 cut(s) 288
FriOI GRGCYC 1 cut(s) 585
Fsp4HI GCNGC 1 cut(s) 288
FspBI CTAG 3 cut(s) 669, 758, 853
GluI GCNGC 1 cut(s) 288
HaeIII GGCC 2 cut(s) 276, 302
HapII CCGG 4 cut(s) 96, 120, 434, 723
HgaI GACGC 1 cut(s) 340
Hin1II CATG 7 cut(s) 67, 194, 214, 232, 263, 349, 487
HindIII AAGCTT 2 cut(s) 8, 745
HinfI GANTC 3 cut(s) 546, 657, 682
HpaII CCGG 4 cut(s) 96, 120, 434, 723
HphI GGTGA 1 cut(s) 218
Hpy166II GTNNAC 2 cut(s) 27, 148
Hpy188I TCNGA 1 cut(s) 656
Hpy188III TCNNGA 3 cut(s) 280, 440, 693
Hpy8I GTNNAC 2 cut(s) 27, 148
Hpy99I CGWCG 1 cut(s) 629
HpyCH4III ACNGT 2 cut(s) 206, 616
HpyCH4IV ACGT 4 cut(s) 197, 381, 624, 651
HpyCH4V TGCA 6 cut(s) 35, 188, 194, 232, 372, 453
HpyF10VI GCNNNNNNNGC 2 cut(s) 324, 503
HpyF3I CTNAG 2 cut(s) 6, 293
HpySE526I ACGT 4 cut(s) 197, 381, 624, 651
Hsp92II CATG 7 cut(s) 67, 194, 214, 232, 263, 349, 487
Ksp22I TGATCA 2 cut(s) 42, 442
Kzo9I GATC 4 cut(s) 42, 358, 442, 665
LmnI GCTCC 1 cut(s) 442
Lsp1109I GCAGC 1 cut(s) 299
LweI GCATC 2 cut(s) 138, 175
MaeI CTAG 3 cut(s) 669, 758, 853
MaeII ACGT 4 cut(s) 197, 381, 624, 651
MaeIII GTNAC 6 cut(s) 68, 98, 134, 206, 643, 760
MalI GATC 4 cut(s) 44, 360, 444, 667
MboI GATC 4 cut(s) 42, 358, 442, 665
MboII GAAGA 3 cut(s) 174, 298, 571
MflI RGATCY 1 cut(s) 665
MhlI GDGCHC 1 cut(s) 585
MluCI AATT 6 cut(s) 396, 424, 676, 697, 811, 847
MlyI GAGTC 2 cut(s) 555, 651
MmeI TCCRAC 1 cut(s) 771
MnlI CCTC 8 cut(s) 152, 212, 287, 314, 387, 557, 560, 829
MroXI GAANNNNTTC 1 cut(s) 23
MseI TTAA 2 cut(s) 534, 573
MslI CAYNNNNRTG 1 cut(s) 488
MspI CCGG 4 cut(s) 96, 120, 434, 723
MspR9I CCNGG 1 cut(s) 598
MvaI CCWGG 1 cut(s) 598
MvnI CGCG 1 cut(s) 611
MwoI GCNNNNNNNGC 2 cut(s) 324, 503
NcoI CCATGG 1 cut(s) 63
NdeII GATC 4 cut(s) 42, 358, 442, 665
NlaIII CATG 7 cut(s) 67, 194, 214, 232, 263, 349, 487
NlaIV GGNNCC 2 cut(s) 432, 438
NmuCI GTSAC 3 cut(s) 98, 206, 760
NspI RCATGY 1 cut(s) 194
PaeR7I CTCGAG 1 cut(s) 278
PdmI GAANNNNTTC 1 cut(s) 23
PfeI GAWTC 1 cut(s) 682
PinAI ACCGGT 1 cut(s) 119
PkrI GCNGC 1 cut(s) 289
PleI GAGTC 2 cut(s) 554, 651
PpsI GAGTC 2 cut(s) 554, 651
Psp124BI GAGCTC 1 cut(s) 585
Psp1406I AACGTT 1 cut(s) 197
Psp6I CCWGG 1 cut(s) 596
PspGI CCWGG 1 cut(s) 596
PspN4I GGNNCC 2 cut(s) 432, 438
PspPI GGNCC 1 cut(s) 301
PstI CTGCAG 1 cut(s) 37
PsuI RGATCY 1 cut(s) 665
RsaI GTAC 2 cut(s) 298, 539
RsaNI GTAC 2 cut(s) 297, 538
RseI CAYNNNNRTG 1 cut(s) 488
SacI GAGCTC 1 cut(s) 585
SaqAI TTAA 2 cut(s) 534, 573
SatI GCNGC 1 cut(s) 288
Sau3AI GATC 4 cut(s) 42, 358, 442, 665
Sau96I GGNCC 1 cut(s) 301
SchI GAGTC 2 cut(s) 555, 651
ScrFI CCNGG 1 cut(s) 598
SduI GDGCHC 1 cut(s) 585
SfaNI GCATC 2 cut(s) 138, 175
SfcI CTRYAG 1 cut(s) 33
Sfr274I CTCGAG 1 cut(s) 278
SlaI CTCGAG 1 cut(s) 278
SmiMI CAYNNNNRTG 1 cut(s) 488
SmlI CTYRAG 2 cut(s) 278, 584
SmoI CTYRAG 2 cut(s) 278, 584
Sse9I AATT 6 cut(s) 396, 424, 676, 697, 811, 847
SsiI CCGC 6 cut(s) 318, 327, 402, 419, 541, 609
SspMI CTAG 3 cut(s) 669, 758, 853
SstI GAGCTC 1 cut(s) 585
StyD4I CCNGG 1 cut(s) 596
StyI CCWWGG 1 cut(s) 63
TaaI ACNGT 2 cut(s) 206, 616
TaiI ACGT 4 cut(s) 200, 384, 627, 654
TaqI TCGA 3 cut(s) 279, 474, 579
TasI AATT 6 cut(s) 396, 424, 676, 697, 811, 847
TfiI GAWTC 1 cut(s) 682
Tru1I TTAA 2 cut(s) 534, 573
Tru9I TTAA 2 cut(s) 534, 573
TscAI CASTG 4 cut(s) 42, 371, 452, 621
TseFI GTSAC 3 cut(s) 98, 206, 760
TseI GCWGC 1 cut(s) 287
Tsp45I GTSAC 3 cut(s) 98, 206, 760
TspDTI ATGAA 2 cut(s) 227, 276
TspGWI ACGGA 1 cut(s) 231
TspRI CASTG 4 cut(s) 42, 371, 452, 621
XapI RAATTY 1 cut(s) 847
XceI RCATGY 1 cut(s) 194
XhoI CTCGAG 1 cut(s) 278
XmnI GAANNNNTTC 1 cut(s) 23
XspI CTAG 3 cut(s) 669, 758, 853
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.