Rh5DG430300

expansin-A23-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001146
Physical Location & Seq
Forward (+)
68775258 .. 68777538
2281 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG430300.1

Sequence Viewer

Length: 501 bp
ATGGGCAGTGATAACATGACTACTTGGTTTACTGGTGCAACTGCATCGTTTTACGGCGACACGCATGGCAGAGGAACCAATAAGGGGGCATGTGGATACGGTGATCTCAAAAAACAAGGCTATGGCTTGGAGACGACATCTCTCAGCGTAGCACTCTTCAACGATGGCCTTGCTTGTGGCGCTTGTTACGCCCTCATATGTGTTGATGATCCAGTGGGGTGCTTACCCAATGCTGGTGCTGTCTTTGTTACTGCAACCAATTTGTGCCCACCAAATTATACTGAAGCCTCTAGAGGGAACCAACAACTGGTGCTACCCACCATCGAAACACTTCAGGTTGTCCCTGCCATGTTAAAAAAAATCGCAGATGAAAAAACTCGGATGGTTCCAGTTATGTATCACAGAGTCCCGTGTGCTAAATATGGAGGGGTTAAGTTCCAAATAGTGAAGGGGGACTCTAGTGTGATGCGTGTGTTATTGTACAACGTTGCCGGTGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

166

Amino Acids

17.6

Weight (kDa)

8.14

Isoelectric Point (pI)

28.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXPB1_D1 PF22514 13 - 94 5.3e-06 EXPB1-like domain 1
DPBB_1 PF03330 46 - 133 1.4e-08 Lytic transglycolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 307
AclI AACGTT 1 cut(s) 486
AclWI GGATC 1 cut(s) 203
AcuI CTGAAG 2 cut(s) 303, 317
AfaI GTAC 1 cut(s) 482
AfiI CCNNNNNNNGG 4 cut(s) 84, 233, 294, 307
AgsI TTSAA 1 cut(s) 160
Alw26I GTCTC 1 cut(s) 125
AlwI GGATC 1 cut(s) 203
AoxI GGCC 1 cut(s) 166
Asp700I GAANNNNTTC 1 cut(s) 330
AspLEI GCGC 1 cut(s) 182
AsuHPI GGTGA 1 cut(s) 113
BaeGI GKGCMC 1 cut(s) 269
BccI CCATC 3 cut(s) 158, 329, 376
BceAI ACGGC 1 cut(s) 70
BcgI CGANNNNNNTGC 4 cut(s) 27, 61, 152, 186
BciVI GTATCC 1 cut(s) 89
BcoDI GTCTC 1 cut(s) 125
BfaI CTAG 2 cut(s) 291, 459
BfoI RGCGCY 1 cut(s) 183
BfuI GTATCC 1 cut(s) 89
BmiI GGNNCC 3 cut(s) 76, 299, 387
BmsI GCATC 2 cut(s) 53, 456
Bsc4I CCNNNNNNNGG 4 cut(s) 84, 233, 294, 307
Bse118I RCCGGY 1 cut(s) 491
Bse1I ACTGG 4 cut(s) 37, 212, 312, 389
BseGI GGATG 1 cut(s) 387
BseLI CCNNNNNNNGG 4 cut(s) 84, 233, 294, 307
BseMII CTCAG 1 cut(s) 157
BseNI ACTGG 4 cut(s) 37, 212, 312, 389
BseSI GKGCMC 1 cut(s) 269
BshFI GGCC 1 cut(s) 168
BsiSI CCGG 1 cut(s) 492
BslFI GGGAC 3 cut(s) 326, 392, 467
BslI CCNNNNNNNGG 4 cut(s) 84, 233, 294, 307
BsmAI GTCTC 1 cut(s) 125
BsmBI CGTCTC 1 cut(s) 125
BsmFI GGGAC 3 cut(s) 326, 392, 467
BsnI GGCC 1 cut(s) 168
Bsp1286I GDGCHC 1 cut(s) 269
Bsp1407I TGTACA 1 cut(s) 480
Bsp143I GATC 2 cut(s) 103, 208
BspANI GGCC 1 cut(s) 168
BspCNI CTCAG 1 cut(s) 156
BspLI GGNNCC 3 cut(s) 76, 299, 387
BspPI GGATC 1 cut(s) 203
BsrFI RCCGGY 1 cut(s) 491
BsrGI TGTACA 1 cut(s) 480
BsrI ACTGG 4 cut(s) 37, 212, 312, 389
BssAI RCCGGY 1 cut(s) 491
BssMI GATC 2 cut(s) 103, 208
Bst4CI ACNGT 1 cut(s) 101
Bst6I CTCTTC 1 cut(s) 161
BstAUI TGTACA 1 cut(s) 480
BstDEI CTNAG 1 cut(s) 143
BstF5I GGATG 1 cut(s) 387
BstH2I RGCGCY 1 cut(s) 183
BstHHI GCGC 1 cut(s) 182
BstKTI GATC 2 cut(s) 106, 211
BstMAI GTCTC 1 cut(s) 125
BstMBI GATC 2 cut(s) 103, 208
BstMWI GCNNNNNNNGC 2 cut(s) 179, 188
BstNSI RCATGY 1 cut(s) 93
BstSLI GKGCMC 1 cut(s) 269
BsuI GTATCC 1 cut(s) 89
BsuRI GGCC 1 cut(s) 168
BtsCI GGATG 1 cut(s) 387
BtsI GCAGTG 1 cut(s) 13
BtsIMutI CAGTG 2 cut(s) 13, 219
CfoI GCGC 1 cut(s) 182
Cfr10I RCCGGY 1 cut(s) 491
Csp6I GTAC 1 cut(s) 481
CviAII CATG 4 cut(s) 16, 65, 90, 349
CviJI RGCY 4 cut(s) 120, 126, 168, 287
CviKI_1 RGCY 4 cut(s) 120, 126, 168, 287
CviQI GTAC 1 cut(s) 481
DdeI CTNAG 1 cut(s) 143
DpnI GATC 2 cut(s) 105, 210
DpnII GATC 2 cut(s) 103, 208
Eam1104I CTCTTC 1 cut(s) 161
EarI CTCTTC 1 cut(s) 161
Eco57I CTGAAG 2 cut(s) 303, 317
Esp3I CGTCTC 1 cut(s) 125
FaeI CATG 4 cut(s) 19, 68, 93, 352
FaqI GGGAC 3 cut(s) 326, 392, 467
FatI CATG 4 cut(s) 15, 64, 89, 348
FauNDI CATATG 1 cut(s) 197
FokI GGATG 1 cut(s) 394
FspBI CTAG 2 cut(s) 291, 459
GlaI GCGC 1 cut(s) 181
HaeII RGCGCY 1 cut(s) 183
HaeIII GGCC 1 cut(s) 168
HapII CCGG 1 cut(s) 492
HhaI GCGC 1 cut(s) 182
Hin1II CATG 4 cut(s) 19, 68, 93, 352
Hin6I GCGC 1 cut(s) 180
HinP1I GCGC 1 cut(s) 180
HinfI GANTC 2 cut(s) 405, 455
HpaII CCGG 1 cut(s) 492
HphI GGTGA 1 cut(s) 113
Hpy166II GTNNAC 1 cut(s) 30
Hpy188I TCNGA 1 cut(s) 381
Hpy188III TCNNGA 1 cut(s) 291
Hpy8I GTNNAC 1 cut(s) 30
HpyAV CCTTC 1 cut(s) 442
HpyCH4III ACNGT 1 cut(s) 101
HpyCH4IV ACGT 1 cut(s) 486
HpyCH4V TGCA 3 cut(s) 38, 44, 254
HpyF10VI GCNNNNNNNGC 2 cut(s) 179, 188
HpyF3I CTNAG 1 cut(s) 143
HpySE526I ACGT 1 cut(s) 486
Hsp92II CATG 4 cut(s) 19, 68, 93, 352
HspAI GCGC 1 cut(s) 180
Kzo9I GATC 2 cut(s) 103, 208
LpnPI CCDG 7 cut(s) 18, 219, 225, 293, 320, 357, 402
LweI GCATC 2 cut(s) 53, 456
MaeI CTAG 2 cut(s) 291, 459
MaeII ACGT 1 cut(s) 486
MaeIII GTNAC 2 cut(s) 185, 247
MalI GATC 2 cut(s) 105, 210
MboI GATC 2 cut(s) 103, 208
MboII GAAGA 1 cut(s) 148
MhlI GDGCHC 1 cut(s) 269
MluCI AATT 2 cut(s) 259, 274
MlyI GAGTC 2 cut(s) 414, 449
MnlI CCTC 5 cut(s) 65, 203, 287, 298, 419
MroXI GAANNNNTTC 1 cut(s) 330
MseI TTAA 2 cut(s) 353, 432
MspI CCGG 1 cut(s) 492
MwoI GCNNNNNNNGC 2 cut(s) 179, 188
NdeI CATATG 1 cut(s) 197
NdeII GATC 2 cut(s) 103, 208
NlaIII CATG 4 cut(s) 19, 68, 93, 352
NlaIV GGNNCC 3 cut(s) 76, 299, 387
NspI RCATGY 1 cut(s) 93
PdmI GAANNNNTTC 1 cut(s) 330
PflMI CCANNNNNTGG 1 cut(s) 307
PleI GAGTC 2 cut(s) 413, 449
PpsI GAGTC 2 cut(s) 413, 449
Psp1406I AACGTT 1 cut(s) 486
PspN4I GGNNCC 3 cut(s) 76, 299, 387
RsaI GTAC 1 cut(s) 482
RsaNI GTAC 1 cut(s) 481
SaqAI TTAA 2 cut(s) 353, 432
Sau3AI GATC 2 cut(s) 103, 208
SchI GAGTC 2 cut(s) 414, 449
SduI GDGCHC 1 cut(s) 269
SetI ASST 2 cut(s) 339, 489
SfaNI GCATC 2 cut(s) 53, 456
Sse9I AATT 2 cut(s) 259, 274
SspMI CTAG 2 cut(s) 291, 459
TaaI ACNGT 1 cut(s) 101
TaiI ACGT 1 cut(s) 489
TaqI TCGA 1 cut(s) 324
TasI AATT 2 cut(s) 259, 274
TatI WGTACW 1 cut(s) 480
Tru1I TTAA 2 cut(s) 353, 432
Tru9I TTAA 2 cut(s) 353, 432
TscAI CASTG 2 cut(s) 13, 219
TspDTI ATGAA 1 cut(s) 384
TspRI CASTG 2 cut(s) 13, 219
Van91I CCANNNNNTGG 1 cut(s) 307
XbaI TCTAGA 1 cut(s) 290
XceI RCATGY 1 cut(s) 93
XmnI GAANNNNTTC 1 cut(s) 330
XspI CTAG 2 cut(s) 291, 459
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.