Rh2AG586400

expansin-A23-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0001146
Physical Location & Seq
Reverse (-)
81866560 .. 81867557
998 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG586400.1

Sequence Viewer

Length: 759 bp
ATGGTTAAGTTCCAGAACTTATTCGCATGGGCAGTGCTCATCATCATCAGCACTCTTTTGGTGCATGCCATGGGCGATGATGCTTGGGAGGATGGAGCCGCAACGTTTTATGGTGACATGAGCGGAGGAGAAACCATGCAGGGGGCTTGTGGATATGGTGATCTCTTCCAACAAGGCTATGGCCTCGAGACAGCAGCTCTAAGCACAGCACTGTTTAACAATGGACTAACTTGTGGTGCTTGTTTTGAGATCATGTGTGTCAATGATCCACAATGGTGCATACCAAATGCTGGTACCATCAAAATCACTGCGACAAACTTTTGTCCACCAAATTATGATCCACATTTTTATCCCTGGTGCAACCCCCCAAGAAAACACTTTGACTTGGCCATGCCCATGTTCGTAAAAATTGCTATCGCCAAAGCTGGGATAATTCCAGTAAAGTTTCGTAGAGTCCCATGTGCTAAGACAGGAGGGGTTAGGTTTGAGCTTAAAGGAAACCCCTACTGGATTACTGCAACCACCTTGAATGTTGGTGGTGCTGGTGATGTTACGGCCGTGAAGATTAAAGGGTCTAATTCCGATTGGCTTCAAATGCAACGCAACTGGGGACAAGTGTGGCAGATCGGAGTTAACTTAGTCGGACAAGGCTTATCGTTTCAAGTCACTACTAGTGATGGGAAGACGGTTGAGCTTGATAATATTGCACCAGGAAATTGGCAATTTGGTCAAACCTATGAAGGGAAAAGCAATTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

252

Amino Acids

27.52

Weight (kDa)

5.89

Isoelectric Point (pI)

18.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXPB1_D1 PF22514 35 - 154 3.7e-06 EXPB1-like domain 1
DPBB_1 PF03330 64 - 149 2.9e-19 Lytic transglycolase
Expansin_C PF01357 160 - 237 8.4e-27 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 293
AccB1I GGYRCC 1 cut(s) 293
AccB7I CCANNNNNTGG 1 cut(s) 290
AccBSI CCGCTC 1 cut(s) 123
AciI CCGC 2 cut(s) 99, 123
AclI AACGTT 1 cut(s) 104
AclWI GGATC 2 cut(s) 260, 332
AcoI YGGCCR 2 cut(s) 387, 555
AfaI GTAC 1 cut(s) 295
AfiI CCNNNNNNNGG 4 cut(s) 141, 290, 426, 741
AgsI TTSAA 3 cut(s) 529, 593, 662
AhlI ACTAGT 1 cut(s) 671
AjnI CCWGG 2 cut(s) 353, 709
AleI CACNNNNGTG 1 cut(s) 274
AluBI AGCT 4 cut(s) 197, 425, 490, 694
AluI AGCT 4 cut(s) 197, 425, 490, 694
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 1 cut(s) 182
AlwI GGATC 2 cut(s) 260, 332
Ama87I CYCGRG 1 cut(s) 185
AoxI GGCC 3 cut(s) 181, 387, 555
ApeKI GCWGC 1 cut(s) 194
Asp700I GAANNNNTTC 1 cut(s) 20
Asp718I GGTACC 1 cut(s) 293
AsuHPI GGTGA 3 cut(s) 125, 170, 557
AvaI CYCGRG 1 cut(s) 185
BalI TGGCCA 1 cut(s) 389
BanI GGYRCC 1 cut(s) 293
BbsI GAAGAC 1 cut(s) 689
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 1 cut(s) 206
BccI CCATC 3 cut(s) 86, 305, 671
BceAI ACGGC 2 cut(s) 542, 570
BciT130I CCWGG 2 cut(s) 355, 711
BcoDI GTCTC 1 cut(s) 182
BcuI ACTAGT 1 cut(s) 671
BfaI CTAG 1 cut(s) 672
BisI GCNGC 2 cut(s) 99, 195
BlsI GCNGC 2 cut(s) 100, 196
Bme1390I CCNGG 2 cut(s) 355, 711
BmeT110I CYCGRG 1 cut(s) 185
BmiI GGNNCC 2 cut(s) 97, 295
BmrFI CCNGG 2 cut(s) 355, 711
BmrI ACTGGG 1 cut(s) 616
BmsI GCATC 1 cut(s) 70
BmuI ACTGGG 1 cut(s) 616
BpiI GAAGAC 1 cut(s) 689
BsaJI CCNNGG 2 cut(s) 69, 353
Bsc4I CCNNNNNNNGG 4 cut(s) 141, 290, 426, 741
Bse1I ACTGG 3 cut(s) 437, 512, 611
BseBI CCWGG 2 cut(s) 355, 711
BseDI CCNNGG 2 cut(s) 69, 353
BseGI GGATG 1 cut(s) 97
BseLI CCNNNNNNNGG 4 cut(s) 141, 290, 426, 741
BseNI ACTGG 3 cut(s) 437, 512, 611
BseRI GAGGAG 1 cut(s) 141
BseX3I CGGCCG 1 cut(s) 555
BseXI GCAGC 1 cut(s) 206
BseYI CCCAGC 1 cut(s) 425
Bsh1285I CGRYCG 1 cut(s) 558
BshFI GGCC 3 cut(s) 183, 389, 557
BshNI GGYRCC 1 cut(s) 293
BsiEI CGRYCG 1 cut(s) 558
BsiHKAI GWGCWC 1 cut(s) 39
BsiHKCI CYCGRG 1 cut(s) 185
BslFI GGGAC 2 cut(s) 440, 624
BslI CCNNNNNNNGG 4 cut(s) 141, 290, 426, 741
BsmAI GTCTC 1 cut(s) 182
BsmFI GGGAC 2 cut(s) 440, 624
BsnI GGCC 3 cut(s) 183, 389, 557
BsoBI CYCGRG 1 cut(s) 185
Bsp1286I GDGCHC 1 cut(s) 39
Bsp143I GATC 5 cut(s) 160, 249, 265, 337, 624
Bsp19I CCATGG 1 cut(s) 69
BspACI CCGC 2 cut(s) 99, 123
BspANI GGCC 3 cut(s) 183, 389, 557
BspLI GGNNCC 2 cut(s) 97, 295
BspPI GGATC 2 cut(s) 260, 332
BspT107I GGYRCC 1 cut(s) 293
BsrBI CCGCTC 1 cut(s) 123
BsrI ACTGG 3 cut(s) 437, 512, 611
BssECI CCNNGG 2 cut(s) 69, 353
BssMI GATC 5 cut(s) 160, 249, 265, 337, 624
BssT1I CCWWGG 1 cut(s) 69
Bst2UI CCWGG 2 cut(s) 355, 711
Bst4CI ACNGT 2 cut(s) 213, 688
Bst6I CTCTTC 1 cut(s) 170
BstC8I GCNNGC 1 cut(s) 66
BstDEI CTNAG 3 cut(s) 200, 465, 637
BstDSI CCRYGG 1 cut(s) 69
BstF5I GGATG 1 cut(s) 97
BstKTI GATC 5 cut(s) 163, 252, 268, 340, 627
BstMAI GTCTC 1 cut(s) 182
BstMBI GATC 5 cut(s) 160, 249, 265, 337, 624
BstMCI CGRYCG 1 cut(s) 558
BstMWI GCNNNNNNNGC 1 cut(s) 595
BstNI CCWGG 2 cut(s) 355, 711
BstNSI RCATGY 1 cut(s) 68
BstSCI CCNGG 2 cut(s) 353, 709
BstV1I GCAGC 1 cut(s) 206
BstV2I GAAGAC 1 cut(s) 689
BstXI CCANNNNNNTGG 1 cut(s) 717
BstZI CGGCCG 1 cut(s) 555
BsuRI GGCC 3 cut(s) 183, 389, 557
BtgI CCRYGG 1 cut(s) 69
BtgZI GCGATG 1 cut(s) 90
BtsCI GGATG 1 cut(s) 97
BtsI GCAGTG 2 cut(s) 39, 306
BtsIMutI CAGTG 3 cut(s) 39, 209, 306
Cac8I GCNNGC 1 cut(s) 66
Csp6I GTAC 1 cut(s) 294
CviAII CATG 9 cut(s) 27, 65, 70, 118, 136, 253, 391, 397, 459
CviQI GTAC 1 cut(s) 294
DdeI CTNAG 3 cut(s) 200, 465, 637
DpnI GATC 5 cut(s) 162, 251, 267, 339, 626
DpnII GATC 5 cut(s) 160, 249, 265, 337, 624
EaeI YGGCCR 2 cut(s) 387, 555
EagI CGGCCG 1 cut(s) 555
Eam1104I CTCTTC 1 cut(s) 170
EarI CTCTTC 1 cut(s) 170
EclXI CGGCCG 1 cut(s) 555
Eco130I CCWWGG 1 cut(s) 69
Eco52I CGGCCG 1 cut(s) 555
Eco88I CYCGRG 1 cut(s) 185
EcoRII CCWGG 2 cut(s) 353, 709
EcoT14I CCWWGG 1 cut(s) 69
ErhI CCWWGG 1 cut(s) 69
FaeI CATG 9 cut(s) 30, 68, 73, 121, 139, 256, 394, 400, 462
FaqI GGGAC 2 cut(s) 440, 624
FatI CATG 9 cut(s) 26, 64, 69, 117, 135, 252, 390, 396, 458
Fnu4HI GCNGC 2 cut(s) 99, 195
FokI GGATG 1 cut(s) 104
Fsp4HI GCNGC 2 cut(s) 99, 195
FspBI CTAG 1 cut(s) 672
GluI GCNGC 2 cut(s) 99, 195
GsaI CCCAGC 1 cut(s) 429
HaeIII GGCC 3 cut(s) 183, 389, 557
Hin1II CATG 9 cut(s) 30, 68, 73, 121, 139, 256, 394, 400, 462
HincII GTYRAC 1 cut(s) 634
HindII GTYRAC 1 cut(s) 634
HinfI GANTC 1 cut(s) 453
HpaI GTTAAC 1 cut(s) 634
HphI GGTGA 3 cut(s) 125, 170, 557
Hpy166II GTNNAC 2 cut(s) 326, 634
Hpy188I TCNGA 3 cut(s) 583, 629, 644
Hpy188III TCNNGA 2 cut(s) 13, 187
Hpy8I GTNNAC 2 cut(s) 326, 634
HpyAV CCTTC 1 cut(s) 734
HpyCH4III ACNGT 2 cut(s) 213, 688
HpyCH4IV ACGT 1 cut(s) 104
HpyCH4V TGCA 7 cut(s) 64, 139, 279, 360, 518, 598, 707
HpyF10VI GCNNNNNNNGC 1 cut(s) 595
HpyF3I CTNAG 3 cut(s) 200, 465, 637
HpySE526I ACGT 1 cut(s) 104
Hsp92II CATG 9 cut(s) 30, 68, 73, 121, 139, 256, 394, 400, 462
KpnI GGTACC 1 cut(s) 297
KspAI GTTAAC 1 cut(s) 634
Kzo9I GATC 5 cut(s) 160, 249, 265, 337, 624
LmnI GCTCC 1 cut(s) 95
Lsp1109I GCAGC 1 cut(s) 206
LweI GCATC 1 cut(s) 70
MaeI CTAG 1 cut(s) 672
MaeII ACGT 1 cut(s) 104
MaeIII GTNAC 3 cut(s) 113, 550, 664
MalI GATC 5 cut(s) 162, 251, 267, 339, 626
MbiI CCGCTC 1 cut(s) 123
MboI GATC 5 cut(s) 160, 249, 265, 337, 624
MboII GAAGA 3 cut(s) 157, 574, 694
MhlI GDGCHC 1 cut(s) 39
MlsI TGGCCA 1 cut(s) 389
MluCI AATT 7 cut(s) 331, 408, 432, 577, 715, 722, 751
MluNI TGGCCA 1 cut(s) 389
MlyI GAGTC 1 cut(s) 462
MmeI TCCRAC 2 cut(s) 193, 622
MnlI CCTC 4 cut(s) 82, 119, 194, 467
Mox20I TGGCCA 1 cut(s) 389
MroXI GAANNNNTTC 1 cut(s) 20
MscI TGGCCA 1 cut(s) 389
MseI TTAA 5 cut(s) 6, 216, 492, 567, 633
MslI CAYNNNNRTG 2 cut(s) 274, 395
Msp20I TGGCCA 1 cut(s) 389
MspR9I CCNGG 2 cut(s) 355, 711
MvaI CCWGG 2 cut(s) 355, 711
MwoI GCNNNNNNNGC 1 cut(s) 595
NcoI CCATGG 1 cut(s) 69
NdeII GATC 5 cut(s) 160, 249, 265, 337, 624
NlaIII CATG 9 cut(s) 30, 68, 73, 121, 139, 256, 394, 400, 462
NlaIV GGNNCC 2 cut(s) 97, 295
NmuCI GTSAC 2 cut(s) 113, 664
NspI RCATGY 1 cut(s) 68
OliI CACNNNNGTG 1 cut(s) 274
PaeI GCATGC 1 cut(s) 68
PaeR7I CTCGAG 1 cut(s) 185
PdmI GAANNNNTTC 1 cut(s) 20
PflMI CCANNNNNTGG 1 cut(s) 290
PkrI GCNGC 2 cut(s) 100, 196
PleI GAGTC 1 cut(s) 461
PpsI GAGTC 1 cut(s) 461
Psp1406I AACGTT 1 cut(s) 104
Psp6I CCWGG 2 cut(s) 353, 709
PspFI CCCAGC 1 cut(s) 425
PspGI CCWGG 2 cut(s) 353, 709
PspN4I GGNNCC 2 cut(s) 97, 295
RsaI GTAC 1 cut(s) 295
RsaNI GTAC 1 cut(s) 294
RseI CAYNNNNRTG 2 cut(s) 274, 395
SaqAI TTAA 5 cut(s) 6, 216, 492, 567, 633
SatI GCNGC 2 cut(s) 99, 195
Sau3AI GATC 5 cut(s) 160, 249, 265, 337, 624
SchI GAGTC 1 cut(s) 462
ScrFI CCNGG 2 cut(s) 355, 711
SduI GDGCHC 1 cut(s) 39
SetI ASST 8 cut(s) 107, 199, 427, 485, 492, 527, 696, 737
SfaNI GCATC 1 cut(s) 70
Sfr274I CTCGAG 1 cut(s) 185
SlaI CTCGAG 1 cut(s) 185
SmiMI CAYNNNNRTG 2 cut(s) 274, 395
SmlI CTYRAG 1 cut(s) 185
SmoI CTYRAG 1 cut(s) 185
SpeI ACTAGT 1 cut(s) 671
SphI GCATGC 1 cut(s) 68
Sse9I AATT 7 cut(s) 331, 408, 432, 577, 715, 722, 751
SsiI CCGC 2 cut(s) 99, 123
SspI AATATT 1 cut(s) 703
SspMI CTAG 1 cut(s) 672
StyD4I CCNGG 2 cut(s) 353, 709
StyI CCWWGG 1 cut(s) 69
TaaI ACNGT 2 cut(s) 213, 688
TaiI ACGT 1 cut(s) 107
TaqI TCGA 1 cut(s) 186
TasI AATT 7 cut(s) 331, 408, 432, 577, 715, 722, 751
TauI GCSGC 1 cut(s) 101
Tru1I TTAA 5 cut(s) 6, 216, 492, 567, 633
Tru9I TTAA 5 cut(s) 6, 216, 492, 567, 633
TscAI CASTG 3 cut(s) 39, 216, 313
TseFI GTSAC 2 cut(s) 113, 664
TseI GCWGC 1 cut(s) 194
Tsp45I GTSAC 2 cut(s) 113, 664
TspDTI ATGAA 1 cut(s) 753
TspRI CASTG 3 cut(s) 39, 216, 313
Van91I CCANNNNNTGG 1 cut(s) 290
XceI RCATGY 1 cut(s) 68
XcmI CCANNNNNNNNNTGG 1 cut(s) 176
XhoI CTCGAG 1 cut(s) 185
XmnI GAANNNNTTC 1 cut(s) 20
XspI CTAG 1 cut(s) 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.