Rh6DG025500

expansin-A23-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0001146
Physical Location & Seq
Forward (+)
2232836 .. 2233881
1046 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG025500.1

Sequence Viewer

Length: 780 bp
ATGCTAGCCATGAGTAAGTTCCAGAACTTGTTCGGAGCGGGGACAGTGTTCATCATCATTCTTTCGGTCCAAACCATGAGCAATCCAATCGGCGAGAGTCCTGACCATGACTGGCACGATGCACATGCAACCTTCTACGGCGACATGAGTGGAAGCGAAACCATGCAGGGGGCTTGCGGATATGGTGATCTCTTCCAACAAGGTTATGGTCTAGATACTGCAGCTCTAAGCACAACATTGTTCAACAATGGATTAACTTGCGGTGCTTGTTTCGAGATCATGTGCGTCAACGATCCACAATGGTGCATCCCTAATGCAGGCACAATCAAAATTACTGCAACAAATTTTTGTCCACCAAACTGGGCTCAAGGAGGCTGGTGCAACCCGCCATACGATCACTTCGACTTGTCCATGCCTATGTTCACAAAAATCGCGCAATATAAAGCTGGAATAGTTCCGGTTAAGTTTCGCCGAGTCCCGTGTGCTAAAACAGGAGGGGTTAAGTTTGAGCTTAAAGGAAACCCCTACTGGATTACTGCTTTGATTTACAATGTGGGTGGTGCCGGTGATGTTACCGCTGTCAGAGTCAAAGGGTCTAATTCCGATTGGCTTCAAATGTCTCGCAATTGGGGCCAAGTTTGGCAGACCAGCGCAAAATTAGTAGGGCAAAGCTTATCATTCCAAGTCACTACCAGTGAAGACAAAACCATTGAGTTTGATGATGTTGCACCAGGGAATTGGCAATTTGGTCAACCATATGAGGGGAATGGTAACTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

259

Amino Acids

28.34

Weight (kDa)

5.1

Isoelectric Point (pI)

29.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXPB1_D1 PF22514 43 - 161 1.1e-06 EXPB1-like domain 1
DPBB_1 PF03330 73 - 156 2.5e-20 Lytic transglycolase
Expansin_C PF01357 167 - 244 2.7e-28 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 560
AccBSI CCGCTC 1 cut(s) 38
AccII CGCG 1 cut(s) 434
AciI CCGC 5 cut(s) 38, 177, 261, 386, 576
AclWI GGATC 1 cut(s) 287
AcsI RAATTY 1 cut(s) 343
AfiI CCNNNNNNNGG 3 cut(s) 168, 317, 761
AgsI TTSAA 2 cut(s) 244, 614
AjnI CCWGG 1 cut(s) 730
AleI CACNNNNGTG 1 cut(s) 301
AluBI AGCT 4 cut(s) 224, 446, 511, 672
AluI AGCT 4 cut(s) 224, 446, 511, 672
Alw26I GTCTC 1 cut(s) 624
AlwI GGATC 1 cut(s) 287
AoxI GGCC 1 cut(s) 631
ApeKI GCWGC 1 cut(s) 221
ApoI RAATTY 1 cut(s) 343
Asp700I GAANNNNTTC 1 cut(s) 29
AspLEI GCGC 2 cut(s) 436, 653
AspS9I GGNCC 2 cut(s) 67, 631
AsuHPI GGTGA 2 cut(s) 197, 578
AsuNHI GCTAGC 1 cut(s) 4
AvaII GGWCC 1 cut(s) 67
BanI GGYRCC 1 cut(s) 560
BanII GRGCYC 1 cut(s) 367
BbsI GAAGAC 1 cut(s) 705
BbvI GCAGC 1 cut(s) 233
BceAI ACGGC 1 cut(s) 154
BcgI CGANNNNNNTGC 4 cut(s) 70, 104, 107, 141
BciT130I CCWGG 1 cut(s) 732
BcoDI GTCTC 1 cut(s) 624
BfaI CTAG 2 cut(s) 5, 212
BfmI CTRYAG 1 cut(s) 219
BisI GCNGC 1 cut(s) 222
BlsI GCNGC 1 cut(s) 223
Bme1390I CCNGG 1 cut(s) 732
Bme18I GGWCC 1 cut(s) 67
BmgT120I GGNCC 2 cut(s) 67, 631
BmiI GGNNCC 2 cut(s) 562, 632
BmrFI CCNGG 1 cut(s) 732
BmrI ACTGGG 1 cut(s) 370
BmsI GCATC 2 cut(s) 109, 315
BmtI GCTAGC 1 cut(s) 8
BmuI ACTGGG 1 cut(s) 370
BpiI GAAGAC 1 cut(s) 705
BpuEI CTTGAG 1 cut(s) 351
BsaJI CCNNGG 1 cut(s) 731
BsaWI WCCGGW 1 cut(s) 457
Bsc4I CCNNNNNNNGG 3 cut(s) 168, 317, 761
Bse118I RCCGGY 1 cut(s) 563
Bse1I ACTGG 4 cut(s) 116, 365, 533, 693
BseBI CCWGG 1 cut(s) 732
BseDI CCNNGG 1 cut(s) 731
BseGI GGATG 1 cut(s) 306
BseLI CCNNNNNNNGG 3 cut(s) 168, 317, 761
BseNI ACTGG 4 cut(s) 116, 365, 533, 693
BseXI GCAGC 1 cut(s) 233
Bsh1236I CGCG 1 cut(s) 434
BshFI GGCC 1 cut(s) 633
BshNI GGYRCC 1 cut(s) 560
BsiSI CCGG 2 cut(s) 458, 564
BslFI GGGAC 2 cut(s) 55, 461
BslI CCNNNNNNNGG 3 cut(s) 168, 317, 761
BsmAI GTCTC 1 cut(s) 624
BsmFI GGGAC 2 cut(s) 55, 461
BsnI GGCC 1 cut(s) 633
Bsp1286I GDGCHC 1 cut(s) 367
Bsp143I GATC 4 cut(s) 187, 276, 292, 394
BspACI CCGC 5 cut(s) 38, 177, 261, 386, 576
BspANI GGCC 1 cut(s) 633
BspFNI CGCG 1 cut(s) 434
BspLI GGNNCC 2 cut(s) 562, 632
BspMAI CTGCAG 1 cut(s) 223
BspOI GCTAGC 1 cut(s) 8
BspPI GGATC 1 cut(s) 287
BspT107I GGYRCC 1 cut(s) 560
BsrBI CCGCTC 1 cut(s) 38
BsrFI RCCGGY 1 cut(s) 563
BsrI ACTGG 4 cut(s) 116, 365, 533, 693
BssAI RCCGGY 1 cut(s) 563
BssECI CCNNGG 1 cut(s) 731
BssMI GATC 4 cut(s) 187, 276, 292, 394
Bst2UI CCWGG 1 cut(s) 732
Bst4CI ACNGT 1 cut(s) 46
Bst6I CTCTTC 1 cut(s) 197
BstC8I GCNNGC 3 cut(s) 6, 175, 319
BstDEI CTNAG 1 cut(s) 227
BstENI CCTNNNNNAGG 1 cut(s) 315
BstF5I GGATG 1 cut(s) 306
BstFNI CGCG 1 cut(s) 434
BstHHI GCGC 2 cut(s) 436, 653
BstKTI GATC 4 cut(s) 190, 279, 295, 397
BstMAI GTCTC 1 cut(s) 624
BstMBI GATC 4 cut(s) 187, 276, 292, 394
BstMWI GCNNNNNNNGC 1 cut(s) 630
BstNI CCWGG 1 cut(s) 732
BstNSI RCATGY 1 cut(s) 128
BstSCI CCNGG 1 cut(s) 730
BstSFI CTRYAG 1 cut(s) 219
BstUI CGCG 1 cut(s) 434
BstV1I GCAGC 1 cut(s) 233
BstV2I GAAGAC 1 cut(s) 705
BstXI CCANNNNNNTGG 2 cut(s) 360, 738
BsuRI GGCC 1 cut(s) 633
BtsCI GGATG 1 cut(s) 306
BtsIMutI CAGTG 2 cut(s) 51, 700
Cac8I GCNNGC 3 cut(s) 6, 175, 319
CfoI GCGC 2 cut(s) 436, 653
Cfr10I RCCGGY 1 cut(s) 563
Cfr13I GGNCC 2 cut(s) 67, 631
CseI GACGC 1 cut(s) 274
CspCI CAANNNNNGTGG 2 cut(s) 538, 573
CviAII CATG 8 cut(s) 10, 76, 107, 125, 145, 163, 280, 412
DdeI CTNAG 1 cut(s) 227
DpnI GATC 4 cut(s) 189, 278, 294, 396
DpnII GATC 4 cut(s) 187, 276, 292, 394
Eam1104I CTCTTC 1 cut(s) 197
EarI CTCTTC 1 cut(s) 197
Eco24I GRGCYC 1 cut(s) 367
Eco47I GGWCC 1 cut(s) 67
EcoNI CCTNNNNNAGG 1 cut(s) 315
EcoRII CCWGG 1 cut(s) 730
EcoT38I GRGCYC 1 cut(s) 367
FaeI CATG 8 cut(s) 13, 79, 110, 128, 148, 166, 283, 415
FaqI GGGAC 2 cut(s) 55, 461
FatI CATG 8 cut(s) 9, 75, 106, 124, 144, 162, 279, 411
FauI CCCGC 2 cut(s) 31, 393
FauNDI CATATG 1 cut(s) 757
Fnu4HI GCNGC 1 cut(s) 222
FokI GGATG 1 cut(s) 293
FriOI GRGCYC 1 cut(s) 367
Fsp4HI GCNGC 1 cut(s) 222
FspBI CTAG 2 cut(s) 5, 212
GlaI GCGC 2 cut(s) 435, 652
GluI GCNGC 1 cut(s) 222
HaeIII GGCC 1 cut(s) 633
HapII CCGG 2 cut(s) 458, 564
HgaI GACGC 1 cut(s) 274
HhaI GCGC 2 cut(s) 436, 653
Hin1II CATG 8 cut(s) 13, 79, 110, 128, 148, 166, 283, 415
Hin6I GCGC 2 cut(s) 434, 651
HinP1I GCGC 2 cut(s) 434, 651
HincII GTYRAC 2 cut(s) 289, 752
HindII GTYRAC 2 cut(s) 289, 752
HindIII AAGCTT 1 cut(s) 670
HinfI GANTC 3 cut(s) 97, 474, 585
HpaII CCGG 2 cut(s) 458, 564
HphI GGTGA 2 cut(s) 197, 578
Hpy166II GTNNAC 4 cut(s) 289, 353, 423, 752
Hpy188I TCNGA 3 cut(s) 35, 584, 604
Hpy188III TCNNGA 4 cut(s) 22, 101, 212, 274
Hpy8I GTNNAC 4 cut(s) 289, 353, 423, 752
HpyAV CCTTC 1 cut(s) 142
HpyCH4III ACNGT 1 cut(s) 46
HpyCH4V TGCA 9 cut(s) 122, 128, 166, 221, 306, 317, 338, 381, 728
HpyF10VI GCNNNNNNNGC 1 cut(s) 630
HpyF3I CTNAG 1 cut(s) 227
Hsp92II CATG 8 cut(s) 13, 79, 110, 128, 148, 166, 283, 415
HspAI GCGC 2 cut(s) 434, 651
Kzo9I GATC 4 cut(s) 187, 276, 292, 394
LmnI GCTCC 1 cut(s) 35
Lsp1109I GCAGC 1 cut(s) 233
LweI GCATC 2 cut(s) 109, 315
MaeI CTAG 2 cut(s) 5, 212
MaeIII GTNAC 3 cut(s) 571, 685, 770
MalI GATC 4 cut(s) 189, 278, 294, 396
MbiI CCGCTC 1 cut(s) 38
MboI GATC 4 cut(s) 187, 276, 292, 394
MboII GAAGA 2 cut(s) 184, 710
MfeI CAATTG 1 cut(s) 625
MhlI GDGCHC 1 cut(s) 367
MluCI AATT 7 cut(s) 330, 343, 598, 625, 656, 736, 743
MlyI GAGTC 3 cut(s) 106, 483, 594
MmeI TCCRAC 1 cut(s) 220
MnlI CCTC 3 cut(s) 365, 488, 754
MroXI GAANNNNTTC 1 cut(s) 29
MseI TTAA 4 cut(s) 254, 462, 501, 513
MslI CAYNNNNRTG 2 cut(s) 301, 416
MspA1I CMGCKG 1 cut(s) 578
MspI CCGG 2 cut(s) 458, 564
MspR9I CCNGG 1 cut(s) 732
MunI CAATTG 1 cut(s) 625
MvaI CCWGG 1 cut(s) 732
MvnI CGCG 1 cut(s) 434
MwoI GCNNNNNNNGC 1 cut(s) 630
NdeI CATATG 1 cut(s) 757
NdeII GATC 4 cut(s) 187, 276, 292, 394
NheI GCTAGC 1 cut(s) 4
NlaIII CATG 8 cut(s) 13, 79, 110, 128, 148, 166, 283, 415
NlaIV GGNNCC 2 cut(s) 562, 632
NmeAIII GCCGAG 1 cut(s) 497
NmuCI GTSAC 1 cut(s) 685
NspI RCATGY 1 cut(s) 128
OliI CACNNNNGTG 1 cut(s) 301
PcsI WCGNNNNNNNCGW 1 cut(s) 399
PdmI GAANNNNTTC 1 cut(s) 29
PkrI GCNGC 1 cut(s) 223
PleI GAGTC 3 cut(s) 105, 482, 593
PpsI GAGTC 3 cut(s) 105, 482, 593
Psp6I CCWGG 1 cut(s) 730
PspGI CCWGG 1 cut(s) 730
PspN4I GGNNCC 2 cut(s) 562, 632
PspPI GGNCC 2 cut(s) 67, 631
PstI CTGCAG 1 cut(s) 223
RseI CAYNNNNRTG 2 cut(s) 301, 416
SaqAI TTAA 4 cut(s) 254, 462, 501, 513
SatI GCNGC 1 cut(s) 222
Sau3AI GATC 4 cut(s) 187, 276, 292, 394
Sau96I GGNCC 2 cut(s) 67, 631
SchI GAGTC 3 cut(s) 106, 483, 594
ScrFI CCNGG 1 cut(s) 732
SduI GDGCHC 1 cut(s) 367
SetI ASST 6 cut(s) 134, 205, 226, 448, 513, 674
SfaNI GCATC 2 cut(s) 109, 315
SfcI CTRYAG 1 cut(s) 219
SinI GGWCC 1 cut(s) 67
SmiMI CAYNNNNRTG 2 cut(s) 301, 416
SmlI CTYRAG 1 cut(s) 366
SmoI CTYRAG 1 cut(s) 366
Sse9I AATT 7 cut(s) 330, 343, 598, 625, 656, 736, 743
SsiI CCGC 5 cut(s) 38, 177, 261, 386, 576
SspMI CTAG 2 cut(s) 5, 212
StyD4I CCNGG 1 cut(s) 730
TaaI ACNGT 1 cut(s) 46
TaqI TCGA 2 cut(s) 273, 402
TaqII GACCGA 1 cut(s) 55
TasI AATT 7 cut(s) 330, 343, 598, 625, 656, 736, 743
Tru1I TTAA 4 cut(s) 254, 462, 501, 513
Tru9I TTAA 4 cut(s) 254, 462, 501, 513
TscAI CASTG 2 cut(s) 51, 700
TseFI GTSAC 1 cut(s) 685
TseI GCWGC 1 cut(s) 221
Tsp45I GTSAC 1 cut(s) 685
TspDTI ATGAA 1 cut(s) 40
TspRI CASTG 2 cut(s) 51, 700
VpaK11BI GGWCC 1 cut(s) 67
XagI CCTNNNNNAGG 1 cut(s) 315
XapI RAATTY 1 cut(s) 343
XbaI TCTAGA 1 cut(s) 211
XceI RCATGY 1 cut(s) 128
XcmI CCANNNNNNNNNTGG 1 cut(s) 203
XmnI GAANNNNTTC 1 cut(s) 29
XspI CTAG 2 cut(s) 5, 212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.