Rmu_sc0006720.1_g000007

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006720.1
OG0001146
Physical Location & Seq
Forward (+)
21169 .. 24316
3148 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006720.1_g000007.1.cds

Sequence Viewer

Length: 1896 bp
atgtacaaggccaagtactttctagaatcttctttttgggaggcagaatctcatccatcaccttcattttcttggcaaagtatttctgaggttagaagtgttctagtcaaaggggcacgttgggaagtgggagatgggaatgatatcctgattgacgacccatggctaccaagagctgctccattttggttattacctgttcagggaggcagagatgcgggttggaagatcagtaatctaatgaataatgaggccagctggaatgaaactttagtccagcaagtttttcatgttgatgatatccccttcattctgtctattcctttgactaatcgaagggttaaggatcgttgggtgtggcattttgactccaaagggatgttttcagttaagagtgcttatagactattgatggaggaagagatggtgcaagagattcctatgcacacacaagatctatggaataaactatggtctaccaaggtcccatgtacggctaaaatttttctatggaaggtgttgagcaattgtttacctacaataattagattgatagagaaacaggttcctttggagattaggccatgtgttctttgtggagtgggagaagagacaattgaacatctttgtaggctttgtccgtttgtgatggcagtgtgggatgccattcctagggttgcacagacggcgtactcttccagggctaggcaattgcagtttatggagtggtttgggtgctgtgcatctaacttgcctcttaatttatgggcagccgttgtttataatttgtggggtatttggaaggagagaaataatagggtgtgggataataagaatatggaggtgaaccaggttgtgttgcttctgtcgactcgtctccaggaataccaaagacaccagggtaaggaccagggtggagggagcctgcgagtagttgaagctttagcagggagggaggcagtctcacttgctattcagcgaggcctctctccagttacctttgagattgattctcttattctttttcatgctactaagcagagagtcattccccatacttccttcattggtcatgtgtatgatgacattacctttcatcttcagcaaatgtcgggttcttactttactcatgcttatcgtcaagccaatgttgtggctcatactttagctcgttttgcttgttcttcgtctagttctttgttttggggcctagagcctccagcttgtattgttgatgtacttttcaaggaacacggctatggtgaaggttttgcttcagcggccgtgttcatctctcttttggttcacggcatgggcagtgataacatgactacttggtttactggtgcaactgcatcgttttacggccacatgcatggcagaggaaccaataagggggcatgtggatacggtgatctcaaaaaacaaggctatggcttggagacgacatctctcagcgtagcactcttcaacgatggctttgcatgtggcgcttgttacgccctcatatgtgttgatgatccagtagggtgcttacccaatgctggtgctgtctttgttactgcaaccaatttgtgcccaccaaattatactgaagcctctagagggaaccaacaactggtgcaacccaccatcgaaacacttcaggttgtccctgccatgtttaaaaaaatcgcagatgaaaaaactcggatgggctctactacttccggttggcttcagatgacacgaattcagggccaaacctggcagattggaaacaaattacaaggccaaagcttgtcgttcctagttaccactagtgatgggaagatggtagagtttgataacattgtgtccgaaaattggcgattcggtgagacctatgaagggatcaagaatttcttaatgggatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

631

Amino Acids

70.99

Weight (kDa)

6.48

Isoelectric Point (pI)

43.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 783
AccB7I CCANNNNNTGG 1 cut(s) 1618
AccI GTMKAC 2 cut(s) 476, 869
AciI CCGC 2 cut(s) 218, 1280
AclWI GGATC 3 cut(s) 354, 1514, 1880
AcoI YGGCCR 2 cut(s) 1281, 1366
AcsI RAATTY 3 cut(s) 501, 1731, 1879
AcuI CTGAAG 5 cut(s) 1085, 1260, 1614, 1628, 1703
AfaI GTAC 5 cut(s) 5, 17, 493, 692, 1239
AgsI TTSAA 4 cut(s) 620, 938, 1246, 1471
AhlI ACTAGT 1 cut(s) 1799
AjnI CCWGG 6 cut(s) 698, 849, 879, 897, 909, 1745
AluBI AGCT 6 cut(s) 176, 258, 941, 1169, 1223, 1779
AluI AGCT 6 cut(s) 176, 258, 941, 1169, 1223, 1779
Alw26I GTCTC 5 cut(s) 605, 881, 967, 1436, 1853
AlwI GGATC 3 cut(s) 354, 1514, 1880
AoxI GGCC 9 cut(s) 9, 252, 581, 982, 1207, 1281, 1366, 1738, 1771
ApeKI GCWGC 2 cut(s) 176, 770
ApoI RAATTY 3 cut(s) 501, 1731, 1879
Asp700I GAANNNNTTC 1 cut(s) 1641
AspA2I CCTAGG 1 cut(s) 671
AspLEI GCGC 1 cut(s) 1493
AspS9I GGNCC 4 cut(s) 484, 907, 1207, 1738
AsuHPI GGTGA 5 cut(s) 51, 856, 1274, 1424, 1868
AvaII GGWCC 2 cut(s) 484, 907
AvrII CCTAGG 1 cut(s) 671
BaeGI GKGCMC 2 cut(s) 118, 1580
BanII GRGCYC 1 cut(s) 1700
BbvI GCAGC 2 cut(s) 163, 782
BceAI ACGGC 7 cut(s) 510, 702, 758, 1268, 1270, 1324, 1381
BcgI CGANNNNNNTGC 4 cut(s) 1338, 1372, 1463, 1497
BciT130I CCWGG 6 cut(s) 700, 851, 881, 899, 911, 1747
BciVI GTATCC 1 cut(s) 1400
BcoDI GTCTC 5 cut(s) 605, 881, 967, 1436, 1853
BcuI ACTAGT 1 cut(s) 1799
BfaI CTAG 9 cut(s) 23, 104, 672, 705, 1191, 1211, 1602, 1790, 1800
BfoI RGCGCY 1 cut(s) 1494
BfuI GTATCC 1 cut(s) 1400
BglII AGATCT 1 cut(s) 454
BisI GCNGC 3 cut(s) 177, 771, 1281
BlnI CCTAGG 1 cut(s) 671
BlsI GCNGC 3 cut(s) 178, 772, 1282
BmcAI AGTACT 1 cut(s) 17
Bme1390I CCNGG 6 cut(s) 700, 851, 881, 899, 911, 1747
Bme18I GGWCC 2 cut(s) 484, 907
BmgT120I GGNCC 4 cut(s) 484, 907, 1207, 1738
BmiI GGNNCC 6 cut(s) 486, 567, 923, 1208, 1387, 1610
BmrFI CCNGG 6 cut(s) 700, 851, 881, 899, 911, 1747
BmsI GCATC 4 cut(s) 205, 652, 752, 1364
BplI GAGNNNNNCTC 2 cut(s) 947, 979
BpmI CTGGAG 3 cut(s) 863, 975, 1203
BsaI GGTCTC 1 cut(s) 1853
BsaJI CCNNGG 6 cut(s) 161, 480, 671, 699, 898, 910
BsaWI WCCGGW 1 cut(s) 1709
Bse1I ACTGG 4 cut(s) 992, 1348, 1523, 1623
BseBI CCWGG 6 cut(s) 700, 851, 881, 899, 911, 1747
BseDI CCNNGG 6 cut(s) 161, 480, 671, 699, 898, 910
BseGI GGATG 4 cut(s) 52, 384, 667, 1698
BseMII CTCAG 2 cut(s) 78, 1468
BseNI ACTGG 4 cut(s) 992, 1348, 1523, 1623
BseSI GKGCMC 2 cut(s) 118, 1580
BseX3I CGGCCG 1 cut(s) 1281
BseXI GCAGC 2 cut(s) 163, 782
Bsh1285I CGRYCG 1 cut(s) 1284
BshFI GGCC 9 cut(s) 11, 254, 583, 984, 1209, 1283, 1368, 1740, 1773
BsiEI CGRYCG 1 cut(s) 1284
BsiSI CCGG 1 cut(s) 1710
BslFI GGGAC 2 cut(s) 470, 1637
BsmAI GTCTC 5 cut(s) 605, 881, 967, 1436, 1853
BsmBI CGTCTC 2 cut(s) 881, 1436
BsmFI GGGAC 2 cut(s) 470, 1637
BsnI GGCC 9 cut(s) 11, 254, 583, 984, 1209, 1283, 1368, 1740, 1773
Bso31I GGTCTC 1 cut(s) 1853
Bsp1286I GDGCHC 3 cut(s) 118, 1580, 1700
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 6 cut(s) 228, 346, 454, 1414, 1519, 1872
Bsp19I CCATGG 1 cut(s) 161
BspACI CCGC 2 cut(s) 218, 1280
BspANI GGCC 9 cut(s) 11, 254, 583, 984, 1209, 1283, 1368, 1740, 1773
BspCNI CTCAG 2 cut(s) 79, 1467
BspLI GGNNCC 6 cut(s) 486, 567, 923, 1208, 1387, 1610
BspPI GGATC 3 cut(s) 354, 1514, 1880
BspTNI GGTCTC 1 cut(s) 1853
BsrGI TGTACA 1 cut(s) 3
BsrI ACTGG 4 cut(s) 992, 1348, 1523, 1623
BssECI CCNNGG 6 cut(s) 161, 480, 671, 699, 898, 910
BssMI GATC 6 cut(s) 228, 346, 454, 1414, 1519, 1872
BssT1I CCWWGG 3 cut(s) 161, 480, 671
Bst2UI CCWGG 6 cut(s) 700, 851, 881, 899, 911, 1747
Bst4CI ACNGT 1 cut(s) 1412
Bst6I CTCTTC 4 cut(s) 414, 603, 700, 1472
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 2 cut(s) 256, 926
BstDEI CTNAG 3 cut(s) 87, 1035, 1454
BstDSI CCRYGG 1 cut(s) 161
BstF5I GGATG 4 cut(s) 52, 384, 667, 1698
BstH2I RGCGCY 1 cut(s) 1494
BstHHI GCGC 1 cut(s) 1493
BstKTI GATC 6 cut(s) 231, 349, 457, 1417, 1522, 1875
BstMAI GTCTC 5 cut(s) 605, 881, 967, 1436, 1853
BstMBI GATC 6 cut(s) 228, 346, 454, 1414, 1519, 1872
BstMCI CGRYCG 1 cut(s) 1284
BstMWI GCNNNNNNNGC 5 cut(s) 686, 1175, 1280, 1490, 1499
BstNI CCWGG 6 cut(s) 700, 851, 881, 899, 911, 1747
BstNSI RCATGY 3 cut(s) 1375, 1404, 1488
BstSCI CCNGG 6 cut(s) 698, 849, 879, 897, 909, 1745
BstSLI GKGCMC 2 cut(s) 118, 1580
BstV1I GCAGC 2 cut(s) 163, 782
BstX2I RGATCY 1 cut(s) 454
BstXI CCANNNNNNTGG 2 cut(s) 1153, 1376
BstYI RGATCY 1 cut(s) 454
BstZI CGGCCG 1 cut(s) 1281
BsuI GTATCC 1 cut(s) 1400
BsuRI GGCC 9 cut(s) 11, 254, 583, 984, 1209, 1283, 1368, 1740, 1773
BtgI CCRYGG 1 cut(s) 161
BtsCI GGATG 4 cut(s) 52, 384, 667, 1698
BtsI GCAGTG 2 cut(s) 660, 1324
BtsIMutI CAGTG 2 cut(s) 660, 1324
Cac8I GCNNGC 2 cut(s) 256, 926
CfoI GCGC 1 cut(s) 1493
Cfr13I GGNCC 4 cut(s) 484, 907, 1207, 1738
CsiI ACCWGGT 1 cut(s) 849
Csp6I GTAC 5 cut(s) 4, 16, 492, 691, 1238
CviQI GTAC 5 cut(s) 4, 16, 492, 691, 1238
DdeI CTNAG 3 cut(s) 87, 1035, 1454
DpnI GATC 6 cut(s) 230, 348, 456, 1416, 1521, 1874
DpnII GATC 6 cut(s) 228, 346, 454, 1414, 1519, 1872
DraI TTTAAA 1 cut(s) 1666
EaeI YGGCCR 2 cut(s) 1281, 1366
EagI CGGCCG 1 cut(s) 1281
Eam1104I CTCTTC 4 cut(s) 414, 603, 700, 1472
EarI CTCTTC 4 cut(s) 414, 603, 700, 1472
EclXI CGGCCG 1 cut(s) 1281
Eco130I CCWWGG 3 cut(s) 161, 480, 671
Eco147I AGGCCT 1 cut(s) 984
Eco24I GRGCYC 1 cut(s) 1700
Eco31I GGTCTC 1 cut(s) 1853
Eco32I GATATC 2 cut(s) 145, 301
Eco47I GGWCC 2 cut(s) 484, 907
Eco52I CGGCCG 1 cut(s) 1281
Eco57I CTGAAG 5 cut(s) 1085, 1260, 1614, 1628, 1703
EcoO109I RGGNCCY 2 cut(s) 484, 1207
EcoRI GAATTC 1 cut(s) 1731
EcoRII CCWGG 6 cut(s) 698, 849, 879, 897, 909, 1745
EcoRV GATATC 2 cut(s) 145, 301
EcoT14I CCWWGG 3 cut(s) 161, 480, 671
EcoT22I ATGCAT 1 cut(s) 1377
EcoT38I GRGCYC 1 cut(s) 1700
ErhI CCWWGG 3 cut(s) 161, 480, 671
Esp3I CGTCTC 2 cut(s) 881, 1436
FalI AAGNNNNNCTT 1 cut(s) 1868
FaqI GGGAC 2 cut(s) 470, 1637
FauI CCCGC 1 cut(s) 211
FauNDI CATATG 1 cut(s) 1508
FblI GTMKAC 2 cut(s) 476, 869
Fnu4HI GCNGC 3 cut(s) 177, 771, 1281
FokI GGATG 4 cut(s) 39, 391, 674, 1705
FriOI GRGCYC 1 cut(s) 1700
Fsp4HI GCNGC 3 cut(s) 177, 771, 1281
FspBI CTAG 9 cut(s) 23, 104, 672, 705, 1191, 1211, 1602, 1790, 1800
GlaI GCGC 1 cut(s) 1492
GluI GCNGC 3 cut(s) 177, 771, 1281
GsuI CTGGAG 3 cut(s) 863, 975, 1203
HaeII RGCGCY 1 cut(s) 1494
HaeIII GGCC 9 cut(s) 11, 254, 583, 984, 1209, 1283, 1368, 1740, 1773
HapII CCGG 1 cut(s) 1710
HhaI GCGC 1 cut(s) 1493
Hin6I GCGC 1 cut(s) 1491
HinP1I GCGC 1 cut(s) 1491
HincII GTYRAC 1 cut(s) 870
HindII GTYRAC 1 cut(s) 870
HindIII AAGCTT 2 cut(s) 939, 1777
HinfI GANTC 8 cut(s) 26, 47, 368, 436, 871, 1010, 1044, 1851
HpaII CCGG 1 cut(s) 1710
HphI GGTGA 5 cut(s) 51, 856, 1274, 1424, 1868
Hpy166II GTNNAC 6 cut(s) 477, 533, 847, 870, 1306, 1341
Hpy188I TCNGA 4 cut(s) 88, 1692, 1722, 1840
Hpy188III TCNNGA 4 cut(s) 23, 148, 1602, 1876
Hpy8I GTNNAC 6 cut(s) 477, 533, 847, 870, 1306, 1341
HpyAV CCTTC 8 cut(s) 72, 316, 330, 508, 796, 1072, 1259, 1862
HpyCH4III ACNGT 1 cut(s) 1412
HpyCH4IV ACGT 1 cut(s) 118
HpyF10VI GCNNNNNNNGC 5 cut(s) 686, 1175, 1280, 1490, 1499
HpyF3I CTNAG 3 cut(s) 87, 1035, 1454
HpySE526I ACGT 1 cut(s) 118
HspAI GCGC 1 cut(s) 1491
Kzo9I GATC 6 cut(s) 228, 346, 454, 1414, 1519, 1872
LmnI GCTCC 2 cut(s) 184, 921
Lsp1109I GCAGC 2 cut(s) 163, 782
LweI GCATC 4 cut(s) 205, 652, 752, 1364
MabI ACCWGGT 1 cut(s) 849
MaeI CTAG 9 cut(s) 23, 104, 672, 705, 1191, 1211, 1602, 1790, 1800
MaeII ACGT 1 cut(s) 118
MaeIII GTNAC 4 cut(s) 994, 1496, 1558, 1792
MalI GATC 6 cut(s) 230, 348, 456, 1416, 1521, 1874
MboI GATC 6 cut(s) 228, 346, 454, 1414, 1519, 1872
MboII GAAGA 9 cut(s) 21, 238, 431, 620, 687, 1091, 1176, 1459, 1822
MfeI CAATTG 3 cut(s) 526, 615, 710
MflI RGATCY 1 cut(s) 454
MhlI GDGCHC 3 cut(s) 118, 1580, 1700
MlyI GAGTC 3 cut(s) 362, 865, 1053
MmeI TCCRAC 1 cut(s) 203
Mph1103I ATGCAT 1 cut(s) 1377
MroXI GAANNNNTTC 1 cut(s) 1641
MseI TTAA 5 cut(s) 342, 390, 759, 1665, 1886
MslI CAYNNNNRTG 4 cut(s) 294, 1077, 1257, 1374
MspA1I CMGCKG 2 cut(s) 258, 1280
MspI CCGG 1 cut(s) 1710
MspR9I CCNGG 6 cut(s) 700, 851, 881, 899, 911, 1747
MunI CAATTG 3 cut(s) 526, 615, 710
MvaI CCWGG 6 cut(s) 700, 851, 881, 899, 911, 1747
MwoI GCNNNNNNNGC 5 cut(s) 686, 1175, 1280, 1490, 1499
NcoI CCATGG 1 cut(s) 161
NdeI CATATG 1 cut(s) 1508
NdeII GATC 6 cut(s) 228, 346, 454, 1414, 1519, 1872
NlaIV GGNNCC 6 cut(s) 486, 567, 923, 1208, 1387, 1610
NsiI ATGCAT 1 cut(s) 1377
NspI RCATGY 3 cut(s) 1375, 1404, 1488
PceI AGGCCT 1 cut(s) 984
PdmI GAANNNNTTC 1 cut(s) 1641
PfeI GAWTC 5 cut(s) 26, 47, 436, 1010, 1851
PflMI CCANNNNNTGG 1 cut(s) 1618
PfoI TCCNGGA 1 cut(s) 879
PkrI GCNGC 3 cut(s) 178, 772, 1282
PleI GAGTC 3 cut(s) 362, 865, 1052
PpsI GAGTC 3 cut(s) 362, 865, 1052
PpuMI RGGWCCY 1 cut(s) 484
PsiI TTATAA 1 cut(s) 783
Psp5II RGGWCCY 1 cut(s) 484
Psp6I CCWGG 6 cut(s) 698, 849, 879, 897, 909, 1745
PspGI CCWGG 6 cut(s) 698, 849, 879, 897, 909, 1745
PspN4I GGNNCC 6 cut(s) 486, 567, 923, 1208, 1387, 1610
PspPI GGNCC 4 cut(s) 484, 907, 1207, 1738
PspPPI RGGWCCY 1 cut(s) 484
PsuI RGATCY 1 cut(s) 454
PvuII CAGCTG 1 cut(s) 258
RsaI GTAC 5 cut(s) 5, 17, 493, 692, 1239
RsaNI GTAC 5 cut(s) 4, 16, 492, 691, 1238
RseI CAYNNNNRTG 4 cut(s) 294, 1077, 1257, 1374
SalI GTCGAC 1 cut(s) 868
SaqAI TTAA 5 cut(s) 342, 390, 759, 1665, 1886
SatI GCNGC 3 cut(s) 177, 771, 1281
Sau3AI GATC 6 cut(s) 228, 346, 454, 1414, 1519, 1872
Sau96I GGNCC 4 cut(s) 484, 907, 1207, 1738
ScaI AGTACT 1 cut(s) 17
SchI GAGTC 3 cut(s) 362, 865, 1053
ScrFI CCNGG 6 cut(s) 700, 851, 881, 899, 911, 1747
SduI GDGCHC 3 cut(s) 118, 1580, 1700
SexAI ACCWGGT 1 cut(s) 849
SfaNI GCATC 4 cut(s) 205, 652, 752, 1364
SinI GGWCC 2 cut(s) 484, 907
SmiMI CAYNNNNRTG 4 cut(s) 294, 1077, 1257, 1374
SpeI ACTAGT 1 cut(s) 1799
SseBI AGGCCT 1 cut(s) 984
SsiI CCGC 2 cut(s) 218, 1280
SspMI CTAG 9 cut(s) 23, 104, 672, 705, 1191, 1211, 1602, 1790, 1800
StuI AGGCCT 1 cut(s) 984
StyD4I CCNGG 6 cut(s) 698, 849, 879, 897, 909, 1745
StyI CCWWGG 3 cut(s) 161, 480, 671
TaaI ACNGT 1 cut(s) 1412
TaiI ACGT 1 cut(s) 121
TaqI TCGA 3 cut(s) 334, 869, 1635
TatI WGTACW 3 cut(s) 3, 15, 1237
TauI GCSGC 1 cut(s) 1283
TfiI GAWTC 5 cut(s) 26, 47, 436, 1010, 1851
Tru1I TTAA 5 cut(s) 342, 390, 759, 1665, 1886
Tru9I TTAA 5 cut(s) 342, 390, 759, 1665, 1886
TscAI CASTG 2 cut(s) 660, 1324
TseI GCWGC 2 cut(s) 176, 770
TspGWI ACGGA 1 cut(s) 630
TspRI CASTG 2 cut(s) 660, 1324
Van91I CCANNNNNTGG 1 cut(s) 1618
VpaK11BI GGWCC 2 cut(s) 484, 907
XapI RAATTY 3 cut(s) 501, 1731, 1879
XbaI TCTAGA 2 cut(s) 22, 1601
XceI RCATGY 3 cut(s) 1375, 1404, 1488
XmaJI CCTAGG 1 cut(s) 671
XmiI GTMKAC 2 cut(s) 476, 869
XmnI GAANNNNTTC 1 cut(s) 1641
XspI CTAG 9 cut(s) 23, 104, 672, 705, 1191, 1211, 1602, 1790, 1800
ZrmI AGTACT 1 cut(s) 17
Zsp2I ATGCAT 1 cut(s) 1377
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.