Rw5G037940

expansin-A23-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001146
Physical Location & Seq
Forward (+)
66927252 .. 66928280
1029 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G037940.1

Sequence Viewer

Length: 348 bp
ATGACTACTTGGTTTACTGGTGCAACTGCATCGTTTTACGGCGACATGCATGGCAGAGGAACCAATAAGGGGGCATGTGGATACGGTGATCTCAAAAAACAAGGCTATGGCTTGGAGACGACATCTCTCAGCGTAGCACTCTTCAACGATGGCCTTGCTTGTGGCGCTTGTTACGCCCTCATATGTGTTGATGATCCAGTAGGGTGCTTACCCAATGCTGGTGCTGTCTTTGTTACTGCAACCAATTTGTGCCCACCAAATTATACTGAAGCCTCTAGAGGGAACCAACAACTGGTGCAAGCCACCAGCCCACCATCGAAACACTTCAGGTTGTCCCTGCCATGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

115

Amino Acids

12.03

Weight (kDa)

6.68

Isoelectric Point (pI)

34.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXPB1_D1 PF22514 9 - 91 1.5e-06 EXPB1-like domain 1
DPBB_1 PF03330 41 - 112 4.7e-09 Lytic transglycolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 292
AclWI GGATC 1 cut(s) 188
AcuI CTGAAG 2 cut(s) 288, 310
AfiI CCNNNNNNNGG 4 cut(s) 69, 218, 279, 292
AgsI TTSAA 1 cut(s) 145
Alw26I GTCTC 1 cut(s) 110
AlwI GGATC 1 cut(s) 188
AoxI GGCC 1 cut(s) 151
Asp700I GAANNNNTTC 1 cut(s) 323
AspLEI GCGC 1 cut(s) 167
AsuHPI GGTGA 1 cut(s) 98
BaeGI GKGCMC 1 cut(s) 254
BccI CCATC 2 cut(s) 143, 322
BceAI ACGGC 1 cut(s) 55
BcgI CGANNNNNNTGC 4 cut(s) 12, 46, 137, 171
BciVI GTATCC 1 cut(s) 74
BcoDI GTCTC 1 cut(s) 110
BfaI CTAG 1 cut(s) 276
BfoI RGCGCY 1 cut(s) 168
BfuI GTATCC 1 cut(s) 74
BmiI GGNNCC 2 cut(s) 61, 284
BmsI GCATC 1 cut(s) 38
Bsc4I CCNNNNNNNGG 4 cut(s) 69, 218, 279, 292
Bse1I ACTGG 3 cut(s) 22, 197, 297
BseLI CCNNNNNNNGG 4 cut(s) 69, 218, 279, 292
BseMII CTCAG 1 cut(s) 142
BseNI ACTGG 3 cut(s) 22, 197, 297
BseSI GKGCMC 1 cut(s) 254
BshFI GGCC 1 cut(s) 153
BslFI GGGAC 1 cut(s) 319
BslI CCNNNNNNNGG 4 cut(s) 69, 218, 279, 292
BsmAI GTCTC 1 cut(s) 110
BsmBI CGTCTC 1 cut(s) 110
BsmFI GGGAC 1 cut(s) 319
BsnI GGCC 1 cut(s) 153
Bsp1286I GDGCHC 1 cut(s) 254
Bsp143I GATC 2 cut(s) 88, 193
BspANI GGCC 1 cut(s) 153
BspCNI CTCAG 1 cut(s) 141
BspLI GGNNCC 2 cut(s) 61, 284
BspPI GGATC 1 cut(s) 188
BsrI ACTGG 3 cut(s) 22, 197, 297
BssMI GATC 2 cut(s) 88, 193
Bst4CI ACNGT 1 cut(s) 86
Bst6I CTCTTC 1 cut(s) 146
BstC8I GCNNGC 1 cut(s) 300
BstDEI CTNAG 1 cut(s) 128
BstH2I RGCGCY 1 cut(s) 168
BstHHI GCGC 1 cut(s) 167
BstKTI GATC 2 cut(s) 91, 196
BstMAI GTCTC 1 cut(s) 110
BstMBI GATC 2 cut(s) 88, 193
BstMWI GCNNNNNNNGC 2 cut(s) 164, 173
BstNSI RCATGY 2 cut(s) 49, 78
BstSLI GKGCMC 1 cut(s) 254
BsuI GTATCC 1 cut(s) 74
BsuRI GGCC 1 cut(s) 153
Cac8I GCNNGC 1 cut(s) 300
CfoI GCGC 1 cut(s) 167
CviAII CATG 4 cut(s) 46, 50, 75, 342
CviJI RGCY 6 cut(s) 105, 111, 153, 272, 302, 309
CviKI_1 RGCY 6 cut(s) 105, 111, 153, 272, 302, 309
DdeI CTNAG 1 cut(s) 128
DpnI GATC 2 cut(s) 90, 195
DpnII GATC 2 cut(s) 88, 193
Eam1104I CTCTTC 1 cut(s) 146
EarI CTCTTC 1 cut(s) 146
Eco57I CTGAAG 2 cut(s) 288, 310
EcoT22I ATGCAT 1 cut(s) 51
Esp3I CGTCTC 1 cut(s) 110
FaeI CATG 4 cut(s) 49, 53, 78, 345
FaiI YATR 8 cut(s) 47, 51, 76, 108, 182, 184, 264, 343
FaqI GGGAC 1 cut(s) 319
FatI CATG 4 cut(s) 45, 49, 74, 341
FauNDI CATATG 1 cut(s) 182
FspBI CTAG 1 cut(s) 276
GlaI GCGC 1 cut(s) 166
HaeII RGCGCY 1 cut(s) 168
HaeIII GGCC 1 cut(s) 153
HhaI GCGC 1 cut(s) 167
Hin1II CATG 4 cut(s) 49, 53, 78, 345
Hin6I GCGC 1 cut(s) 165
HinP1I GCGC 1 cut(s) 165
HphI GGTGA 1 cut(s) 98
Hpy166II GTNNAC 1 cut(s) 15
Hpy188III TCNNGA 1 cut(s) 276
Hpy8I GTNNAC 1 cut(s) 15
HpyCH4III ACNGT 1 cut(s) 86
HpyCH4V TGCA 5 cut(s) 23, 29, 49, 239, 298
HpyF10VI GCNNNNNNNGC 2 cut(s) 164, 173
HpyF3I CTNAG 1 cut(s) 128
Hsp92II CATG 4 cut(s) 49, 53, 78, 345
HspAI GCGC 1 cut(s) 165
Kzo9I GATC 2 cut(s) 88, 193
LpnPI CCDG 6 cut(s) 3, 204, 210, 278, 313, 319
LweI GCATC 1 cut(s) 38
MaeI CTAG 1 cut(s) 276
MaeIII GTNAC 2 cut(s) 170, 232
MalI GATC 2 cut(s) 90, 195
MboI GATC 2 cut(s) 88, 193
MboII GAAGA 1 cut(s) 133
MhlI GDGCHC 1 cut(s) 254
MluCI AATT 2 cut(s) 244, 259
MnlI CCTC 4 cut(s) 50, 188, 272, 283
Mph1103I ATGCAT 1 cut(s) 51
MroXI GAANNNNTTC 1 cut(s) 323
MseI TTAA 1 cut(s) 346
MwoI GCNNNNNNNGC 2 cut(s) 164, 173
NdeI CATATG 1 cut(s) 182
NdeII GATC 2 cut(s) 88, 193
NlaIII CATG 4 cut(s) 49, 53, 78, 345
NlaIV GGNNCC 2 cut(s) 61, 284
NsiI ATGCAT 1 cut(s) 51
NspI RCATGY 2 cut(s) 49, 78
PdmI GAANNNNTTC 1 cut(s) 323
PflMI CCANNNNNTGG 1 cut(s) 292
PspN4I GGNNCC 2 cut(s) 61, 284
SaqAI TTAA 1 cut(s) 346
Sau3AI GATC 2 cut(s) 88, 193
SduI GDGCHC 1 cut(s) 254
SetI ASST 1 cut(s) 332
SfaNI GCATC 1 cut(s) 38
Sse9I AATT 2 cut(s) 244, 259
SspMI CTAG 1 cut(s) 276
TaaI ACNGT 1 cut(s) 86
TaqI TCGA 1 cut(s) 317
TasI AATT 2 cut(s) 244, 259
Tru1I TTAA 1 cut(s) 346
Tru9I TTAA 1 cut(s) 346
Van91I CCANNNNNTGG 1 cut(s) 292
XbaI TCTAGA 1 cut(s) 275
XceI RCATGY 2 cut(s) 49, 78
XmnI GAANNNNTTC 1 cut(s) 323
XspI CTAG 1 cut(s) 276
Zsp2I ATGCAT 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.