Rroxscaffold_1G00019120

expansin-A23-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001146
Physical Location & Seq
Reverse (-)
23315917 .. 23317302
1386 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00019120.1

Sequence Viewer

Length: 780 bp
ATGGTGAAGGTTTTGCTTACAGCGGCCGCGTTCATCTCTCTTTTGGTTCACGGCATGGGCGGTGATAACATGACTACTTGGTTTACTGGTGCAACTGCATCGTTTTACGGCGACATGCATGGCAGAGGAACCAATAAGGGGGCATGTGGATACGGTGATCTCAAAAAACAGGGCTATGGCTTGGAGACGACATCTCTCAGCGCAGCACTCTTCAACGATGGCCTTGCTTGTGGCGCTTGTTACGCCCTCATATGTGTTGATGATCCAGTAGGGTGCTTACCCAATGCTGGTGCTGTCTTTGTTACTGCAACCAATTTTTGCCCACCAAATTATACTAAGCCTCTAGAGGGAACCAACAACTGGTGCAACCCACCATCGAAACACTTCAGGTTGTCCCTGCCTATGTTTAAAAAAATCGCAGATGAAAAAACTCGGATGGTACCAGTTATGTATCACAGAGTCCCGTGTGCTAAATATGGAGGGGTTAAGTTCCAAATAGTGAAGGGGGACTCTAGTGTGATGTGTGTGTTATTGTACAACGTTGCCGGTGCTGGTGATGTTTCTGATGTCAAAATTAAGGACTCTAATACTTCCGGTTGGCTTCAGATGACACAAATTCAGGGCCAAACCTGGCAGATCGGAAACACATTACAAGGCCAAAGCTTGTCGTTCCTAGTTACCACTAGTGATGGGAAGATGGTAGGGTTTGATAACATTGTGTCCGAAAATTGGCGATTCGGTGAGACCTATGATGGGATCAAGAATTTCTTAATGGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

259

Amino Acids

27.93

Weight (kDa)

8.03

Isoelectric Point (pI)

22.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 64 - 151 9.3e-15 Lytic transglycolase
Expansin_C PF01357 162 - 240 2.4e-19 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 439
AccB1I GGYRCC 1 cut(s) 439
AccB7I CCANNNNNTGG 1 cut(s) 360
AccII CGCG 1 cut(s) 29
AciI CCGC 3 cut(s) 23, 27, 60
AclI AACGTT 1 cut(s) 540
AclWI GGATC 2 cut(s) 257, 764
AcoI YGGCCR 1 cut(s) 24
AcsI RAATTY 2 cut(s) 615, 763
AcuI CTGAAG 2 cut(s) 370, 587
AfaI GTAC 2 cut(s) 441, 536
AfiI CCNNNNNNNGG 6 cut(s) 138, 287, 347, 360, 729, 753
AgsI TTSAA 1 cut(s) 214
AhlI ACTAGT 1 cut(s) 683
AjnI CCWGG 1 cut(s) 629
AluBI AGCT 1 cut(s) 663
AluI AGCT 1 cut(s) 663
Alw26I GTCTC 2 cut(s) 179, 737
AlwI GGATC 2 cut(s) 257, 764
AoxI GGCC 4 cut(s) 24, 220, 622, 655
ApeKI GCWGC 1 cut(s) 203
ApoI RAATTY 2 cut(s) 615, 763
Asp700I GAANNNNTTC 1 cut(s) 383
Asp718I GGTACC 1 cut(s) 439
AspLEI GCGC 2 cut(s) 203, 236
AspS9I GGNCC 1 cut(s) 622
AsuHPI GGTGA 5 cut(s) 16, 74, 167, 566, 752
BanI GGYRCC 1 cut(s) 439
BbvI GCAGC 1 cut(s) 215
BccI CCATC 6 cut(s) 212, 382, 430, 683, 691, 746
BceAI ACGGC 2 cut(s) 67, 124
BcgI CGANNNNNNTGC 4 cut(s) 81, 115, 206, 240
BciT130I CCWGG 1 cut(s) 631
BciVI GTATCC 1 cut(s) 143
BcoDI GTCTC 2 cut(s) 179, 737
BcuI ACTAGT 1 cut(s) 683
BfaI CTAG 4 cut(s) 344, 513, 674, 684
BfoI RGCGCY 1 cut(s) 237
BfuI GTATCC 1 cut(s) 143
BisI GCNGC 3 cut(s) 24, 27, 204
BlsI GCNGC 3 cut(s) 25, 28, 205
Bme1390I CCNGG 1 cut(s) 631
BmgT120I GGNCC 1 cut(s) 622
BmiI GGNNCC 3 cut(s) 130, 352, 441
BmrFI CCNGG 1 cut(s) 631
BmsI GCATC 1 cut(s) 107
BsaI GGTCTC 1 cut(s) 737
BsaWI WCCGGW 1 cut(s) 593
Bsc4I CCNNNNNNNGG 6 cut(s) 138, 287, 347, 360, 729, 753
Bse118I RCCGGY 1 cut(s) 545
Bse1I ACTGG 4 cut(s) 91, 266, 365, 443
BseBI CCWGG 1 cut(s) 631
BseGI GGATG 1 cut(s) 441
BseLI CCNNNNNNNGG 6 cut(s) 138, 287, 347, 360, 729, 753
BseMII CTCAG 1 cut(s) 211
BseNI ACTGG 4 cut(s) 91, 266, 365, 443
BseX3I CGGCCG 1 cut(s) 24
BseXI GCAGC 1 cut(s) 215
Bsh1236I CGCG 1 cut(s) 29
Bsh1285I CGRYCG 1 cut(s) 27
BshFI GGCC 4 cut(s) 26, 222, 624, 657
BshNI GGYRCC 1 cut(s) 439
BsiEI CGRYCG 1 cut(s) 27
BsiSI CCGG 2 cut(s) 546, 594
BslFI GGGAC 3 cut(s) 379, 446, 521
BslI CCNNNNNNNGG 6 cut(s) 138, 287, 347, 360, 729, 753
BsmAI GTCTC 2 cut(s) 179, 737
BsmBI CGTCTC 1 cut(s) 179
BsmFI GGGAC 3 cut(s) 379, 446, 521
BsnI GGCC 4 cut(s) 26, 222, 624, 657
Bso31I GGTCTC 1 cut(s) 737
Bsp1407I TGTACA 1 cut(s) 534
Bsp143I GATC 4 cut(s) 157, 262, 636, 756
BspACI CCGC 3 cut(s) 23, 27, 60
BspANI GGCC 4 cut(s) 26, 222, 624, 657
BspCNI CTCAG 1 cut(s) 210
BspFNI CGCG 1 cut(s) 29
BspLI GGNNCC 3 cut(s) 130, 352, 441
BspPI GGATC 2 cut(s) 257, 764
BspT107I GGYRCC 1 cut(s) 439
BspTNI GGTCTC 1 cut(s) 737
BsrFI RCCGGY 1 cut(s) 545
BsrGI TGTACA 1 cut(s) 534
BsrI ACTGG 4 cut(s) 91, 266, 365, 443
BssAI RCCGGY 1 cut(s) 545
BssMI GATC 4 cut(s) 157, 262, 636, 756
Bst2UI CCWGG 1 cut(s) 631
Bst4CI ACNGT 1 cut(s) 155
Bst6I CTCTTC 1 cut(s) 215
BstAUI TGTACA 1 cut(s) 534
BstDEI CTNAG 2 cut(s) 197, 336
BstF5I GGATG 1 cut(s) 441
BstFNI CGCG 1 cut(s) 29
BstH2I RGCGCY 1 cut(s) 237
BstHHI GCGC 2 cut(s) 203, 236
BstKTI GATC 4 cut(s) 160, 265, 639, 759
BstMAI GTCTC 2 cut(s) 179, 737
BstMBI GATC 4 cut(s) 157, 262, 636, 756
BstMCI CGRYCG 1 cut(s) 27
BstMWI GCNNNNNNNGC 2 cut(s) 233, 242
BstNI CCWGG 1 cut(s) 631
BstNSI RCATGY 2 cut(s) 118, 147
BstSCI CCNGG 1 cut(s) 629
BstUI CGCG 1 cut(s) 29
BstV1I GCAGC 1 cut(s) 215
BstZI CGGCCG 1 cut(s) 24
BsuI GTATCC 1 cut(s) 143
BsuRI GGCC 4 cut(s) 26, 222, 624, 657
BtsCI GGATG 1 cut(s) 441
CciNI GCGGCCGC 1 cut(s) 24
CfoI GCGC 2 cut(s) 203, 236
Cfr10I RCCGGY 1 cut(s) 545
Cfr13I GGNCC 1 cut(s) 622
Csp6I GTAC 2 cut(s) 440, 535
CviAII CATG 5 cut(s) 55, 70, 115, 119, 144
CviJI RGCY 9 cut(s) 26, 174, 180, 222, 340, 601, 624, 657, 663
CviKI_1 RGCY 9 cut(s) 26, 174, 180, 222, 340, 601, 624, 657, 663
CviQI GTAC 2 cut(s) 440, 535
DdeI CTNAG 2 cut(s) 197, 336
DpnI GATC 4 cut(s) 159, 264, 638, 758
DpnII GATC 4 cut(s) 157, 262, 636, 756
DraI TTTAAA 1 cut(s) 409
EaeI YGGCCR 1 cut(s) 24
EagI CGGCCG 1 cut(s) 24
Eam1104I CTCTTC 1 cut(s) 215
EarI CTCTTC 1 cut(s) 215
EclXI CGGCCG 1 cut(s) 24
Eco31I GGTCTC 1 cut(s) 737
Eco52I CGGCCG 1 cut(s) 24
Eco57I CTGAAG 2 cut(s) 370, 587
EcoRII CCWGG 1 cut(s) 629
EcoT22I ATGCAT 1 cut(s) 120
Esp3I CGTCTC 1 cut(s) 179
FaeI CATG 5 cut(s) 58, 73, 118, 122, 147
FalI AAGNNNNNCTT 1 cut(s) 752
FaqI GGGAC 3 cut(s) 379, 446, 521
FatI CATG 5 cut(s) 54, 69, 114, 118, 143
FauNDI CATATG 1 cut(s) 251
Fnu4HI GCNGC 3 cut(s) 24, 27, 204
FokI GGATG 1 cut(s) 448
Fsp4HI GCNGC 3 cut(s) 24, 27, 204
FspBI CTAG 4 cut(s) 344, 513, 674, 684
GlaI GCGC 2 cut(s) 202, 235
GluI GCNGC 3 cut(s) 24, 27, 204
HaeII RGCGCY 1 cut(s) 237
HaeIII GGCC 4 cut(s) 26, 222, 624, 657
HapII CCGG 2 cut(s) 546, 594
HhaI GCGC 2 cut(s) 203, 236
Hin1II CATG 5 cut(s) 58, 73, 118, 122, 147
Hin6I GCGC 2 cut(s) 201, 234
HinP1I GCGC 2 cut(s) 201, 234
HindIII AAGCTT 1 cut(s) 661
HinfI GANTC 4 cut(s) 459, 509, 581, 735
HpaII CCGG 2 cut(s) 546, 594
HphI GGTGA 5 cut(s) 16, 74, 167, 566, 752
Hpy166II GTNNAC 2 cut(s) 49, 84
Hpy188I TCNGA 5 cut(s) 435, 565, 606, 641, 724
Hpy188III TCNNGA 2 cut(s) 344, 760
Hpy8I GTNNAC 2 cut(s) 49, 84
HpyAV CCTTC 1 cut(s) 496
HpyCH4III ACNGT 1 cut(s) 155
HpyCH4IV ACGT 1 cut(s) 540
HpyCH4V TGCA 5 cut(s) 92, 98, 118, 308, 366
HpyF10VI GCNNNNNNNGC 2 cut(s) 233, 242
HpyF3I CTNAG 2 cut(s) 197, 336
HpySE526I ACGT 1 cut(s) 540
Hsp92II CATG 5 cut(s) 58, 73, 118, 122, 147
HspAI GCGC 2 cut(s) 201, 234
KpnI GGTACC 1 cut(s) 443
Kzo9I GATC 4 cut(s) 157, 262, 636, 756
Lsp1109I GCAGC 1 cut(s) 215
LweI GCATC 1 cut(s) 107
MaeI CTAG 4 cut(s) 344, 513, 674, 684
MaeII ACGT 1 cut(s) 540
MaeIII GTNAC 3 cut(s) 239, 301, 676
MalI GATC 4 cut(s) 159, 264, 638, 758
MboI GATC 4 cut(s) 157, 262, 636, 756
MboII GAAGA 2 cut(s) 202, 706
MluCI AATT 6 cut(s) 313, 328, 573, 615, 727, 763
MlyI GAGTC 3 cut(s) 468, 503, 575
MnlI CCTC 5 cut(s) 119, 257, 340, 351, 473
Mph1103I ATGCAT 1 cut(s) 120
MroXI GAANNNNTTC 1 cut(s) 383
MseI TTAA 4 cut(s) 408, 486, 576, 770
MspA1I CMGCKG 1 cut(s) 23
MspI CCGG 2 cut(s) 546, 594
MspR9I CCNGG 1 cut(s) 631
MvaI CCWGG 1 cut(s) 631
MvnI CGCG 1 cut(s) 29
MwoI GCNNNNNNNGC 2 cut(s) 233, 242
NdeI CATATG 1 cut(s) 251
NdeII GATC 4 cut(s) 157, 262, 636, 756
NlaIII CATG 5 cut(s) 58, 73, 118, 122, 147
NlaIV GGNNCC 3 cut(s) 130, 352, 441
NotI GCGGCCGC 1 cut(s) 24
NsiI ATGCAT 1 cut(s) 120
NspI RCATGY 2 cut(s) 118, 147
PdmI GAANNNNTTC 1 cut(s) 383
PfeI GAWTC 1 cut(s) 735
PflMI CCANNNNNTGG 1 cut(s) 360
PkrI GCNGC 3 cut(s) 25, 28, 205
PleI GAGTC 3 cut(s) 467, 503, 575
PpsI GAGTC 3 cut(s) 467, 503, 575
Psp1406I AACGTT 1 cut(s) 540
Psp6I CCWGG 1 cut(s) 629
PspGI CCWGG 1 cut(s) 629
PspN4I GGNNCC 3 cut(s) 130, 352, 441
PspPI GGNCC 1 cut(s) 622
RsaI GTAC 2 cut(s) 441, 536
RsaNI GTAC 2 cut(s) 440, 535
SaqAI TTAA 4 cut(s) 408, 486, 576, 770
SatI GCNGC 3 cut(s) 24, 27, 204
Sau3AI GATC 4 cut(s) 157, 262, 636, 756
Sau96I GGNCC 1 cut(s) 622
SchI GAGTC 3 cut(s) 468, 503, 575
ScrFI CCNGG 1 cut(s) 631
SetI ASST 6 cut(s) 12, 392, 543, 632, 665, 749
SfaNI GCATC 1 cut(s) 107
SpeI ACTAGT 1 cut(s) 683
Sse9I AATT 6 cut(s) 313, 328, 573, 615, 727, 763
SsiI CCGC 3 cut(s) 23, 27, 60
SspMI CTAG 4 cut(s) 344, 513, 674, 684
StyD4I CCNGG 1 cut(s) 629
TaaI ACNGT 1 cut(s) 155
TaiI ACGT 1 cut(s) 543
TaqI TCGA 1 cut(s) 377
TasI AATT 6 cut(s) 313, 328, 573, 615, 727, 763
TatI WGTACW 1 cut(s) 534
TauI GCSGC 2 cut(s) 26, 29
TfiI GAWTC 1 cut(s) 735
Tru1I TTAA 4 cut(s) 408, 486, 576, 770
Tru9I TTAA 4 cut(s) 408, 486, 576, 770
TseI GCWGC 1 cut(s) 203
TspDTI ATGAA 2 cut(s) 22, 438
Van91I CCANNNNNTGG 1 cut(s) 360
XapI RAATTY 2 cut(s) 615, 763
XbaI TCTAGA 1 cut(s) 343
XceI RCATGY 2 cut(s) 118, 147
XmnI GAANNNNTTC 1 cut(s) 383
XspI CTAG 4 cut(s) 344, 513, 674, 684
Zsp2I ATGCAT 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.