RchiOBHm_Chr5g0061551

expansin-A23-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001146
Physical Location & Seq
Forward (+)
67058668 .. 67059687
1020 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33792

Sequence Viewer

Length: 411 bp
ATGGTGAAGGTTTTGCTTACAGCGGCCGTGTTCATCTCTCTTTTGGTTCACGGCATGGGCGGTGATAACATGACTACTTGGTTTACTGGTGCAACTGCATCGTTTTACGGCGACATGCATGGCAGAGGAATCAATAAGGGGGCATGTGGATACGGTGATCTCAAAAAACAAGGCTATGGCTTGGAGACGACATCTCTCAGCGTAGCACTCTTCAACGATGGCCTTGCTGGTGGCGCTTGTTACGCCCTCATATGTGTTGATGATCCAGTAGGGTGCTTACCCAATGCTGGTGCTGTCTTTGTTACTGCAATCAATTTGTGCCCACCAAATTATACTGAAGCCTCTAGAGGGAACCAACAACTGGTGCAACCCACCATCGAAACACTTCAGGTTGTCCCTGCCTATGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

136

Amino Acids

14.17

Weight (kDa)

5.03

Isoelectric Point (pI)

32.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 361
AciI CCGC 2 cut(s) 23, 60
AclWI GGATC 1 cut(s) 257
AcoI YGGCCR 1 cut(s) 24
AcuI CTGAAG 2 cut(s) 357, 371
AfiI CCNNNNNNNGG 3 cut(s) 287, 348, 361
AgsI TTSAA 1 cut(s) 214
Alw26I GTCTC 1 cut(s) 179
AlwI GGATC 1 cut(s) 257
AoxI GGCC 2 cut(s) 24, 220
Asp700I GAANNNNTTC 1 cut(s) 384
AspLEI GCGC 1 cut(s) 236
AsuHPI GGTGA 3 cut(s) 16, 74, 167
BaeGI GKGCMC 1 cut(s) 323
BccI CCATC 2 cut(s) 212, 383
BceAI ACGGC 3 cut(s) 11, 67, 124
BcgI CGANNNNNNTGC 4 cut(s) 81, 115, 206, 240
BciVI GTATCC 1 cut(s) 143
BcoDI GTCTC 1 cut(s) 179
BfaI CTAG 1 cut(s) 345
BfoI RGCGCY 1 cut(s) 237
BfuI GTATCC 1 cut(s) 143
BisI GCNGC 1 cut(s) 24
BlsI GCNGC 1 cut(s) 25
BmiI GGNNCC 1 cut(s) 353
BmsI GCATC 1 cut(s) 107
Bsc4I CCNNNNNNNGG 3 cut(s) 287, 348, 361
Bse1I ACTGG 3 cut(s) 91, 266, 366
BseLI CCNNNNNNNGG 3 cut(s) 287, 348, 361
BseMII CTCAG 1 cut(s) 211
BseNI ACTGG 3 cut(s) 91, 266, 366
BseSI GKGCMC 1 cut(s) 323
BseX3I CGGCCG 1 cut(s) 24
Bsh1285I CGRYCG 1 cut(s) 27
BshFI GGCC 2 cut(s) 26, 222
BsiEI CGRYCG 1 cut(s) 27
BslFI GGGAC 1 cut(s) 380
BslI CCNNNNNNNGG 3 cut(s) 287, 348, 361
BsmAI GTCTC 1 cut(s) 179
BsmBI CGTCTC 1 cut(s) 179
BsmFI GGGAC 1 cut(s) 380
BsnI GGCC 2 cut(s) 26, 222
Bsp1286I GDGCHC 1 cut(s) 323
Bsp143I GATC 2 cut(s) 157, 262
BspACI CCGC 2 cut(s) 23, 60
BspANI GGCC 2 cut(s) 26, 222
BspCNI CTCAG 1 cut(s) 210
BspLI GGNNCC 1 cut(s) 353
BspPI GGATC 1 cut(s) 257
BsrI ACTGG 3 cut(s) 91, 266, 366
BssMI GATC 2 cut(s) 157, 262
Bst4CI ACNGT 1 cut(s) 155
Bst6I CTCTTC 1 cut(s) 215
BstDEI CTNAG 1 cut(s) 197
BstH2I RGCGCY 1 cut(s) 237
BstHHI GCGC 1 cut(s) 236
BstKTI GATC 2 cut(s) 160, 265
BstMAI GTCTC 1 cut(s) 179
BstMBI GATC 2 cut(s) 157, 262
BstMCI CGRYCG 1 cut(s) 27
BstMWI GCNNNNNNNGC 2 cut(s) 233, 242
BstNSI RCATGY 2 cut(s) 118, 147
BstSLI GKGCMC 1 cut(s) 323
BstZI CGGCCG 1 cut(s) 24
BsuI GTATCC 1 cut(s) 143
BsuRI GGCC 2 cut(s) 26, 222
CfoI GCGC 1 cut(s) 236
CviAII CATG 5 cut(s) 55, 70, 115, 119, 144
CviJI RGCY 5 cut(s) 26, 174, 180, 222, 341
CviKI_1 RGCY 5 cut(s) 26, 174, 180, 222, 341
DdeI CTNAG 1 cut(s) 197
DpnI GATC 2 cut(s) 159, 264
DpnII GATC 2 cut(s) 157, 262
EaeI YGGCCR 1 cut(s) 24
EagI CGGCCG 1 cut(s) 24
Eam1104I CTCTTC 1 cut(s) 215
EarI CTCTTC 1 cut(s) 215
EclXI CGGCCG 1 cut(s) 24
Eco52I CGGCCG 1 cut(s) 24
Eco57I CTGAAG 2 cut(s) 357, 371
EcoT22I ATGCAT 1 cut(s) 120
Esp3I CGTCTC 1 cut(s) 179
FaeI CATG 5 cut(s) 58, 73, 118, 122, 147
FaqI GGGAC 1 cut(s) 380
FatI CATG 5 cut(s) 54, 69, 114, 118, 143
FauNDI CATATG 1 cut(s) 251
Fnu4HI GCNGC 1 cut(s) 24
Fsp4HI GCNGC 1 cut(s) 24
FspBI CTAG 1 cut(s) 345
GlaI GCGC 1 cut(s) 235
GluI GCNGC 1 cut(s) 24
HaeII RGCGCY 1 cut(s) 237
HaeIII GGCC 2 cut(s) 26, 222
HhaI GCGC 1 cut(s) 236
Hin1II CATG 5 cut(s) 58, 73, 118, 122, 147
Hin6I GCGC 1 cut(s) 234
HinP1I GCGC 1 cut(s) 234
HinfI GANTC 1 cut(s) 129
HphI GGTGA 3 cut(s) 16, 74, 167
Hpy166II GTNNAC 2 cut(s) 49, 84
Hpy188III TCNNGA 1 cut(s) 345
Hpy8I GTNNAC 2 cut(s) 49, 84
HpyCH4III ACNGT 1 cut(s) 155
HpyCH4V TGCA 5 cut(s) 92, 98, 118, 308, 367
HpyF10VI GCNNNNNNNGC 2 cut(s) 233, 242
HpyF3I CTNAG 1 cut(s) 197
Hsp92II CATG 5 cut(s) 58, 73, 118, 122, 147
HspAI GCGC 1 cut(s) 234
Kzo9I GATC 2 cut(s) 157, 262
LpnPI CCDG 6 cut(s) 72, 213, 273, 279, 347, 374
LweI GCATC 1 cut(s) 107
MaeI CTAG 1 cut(s) 345
MaeIII GTNAC 2 cut(s) 239, 301
MalI GATC 2 cut(s) 159, 264
MboI GATC 2 cut(s) 157, 262
MboII GAAGA 1 cut(s) 202
MhlI GDGCHC 1 cut(s) 323
MluCI AATT 2 cut(s) 313, 328
MnlI CCTC 4 cut(s) 119, 257, 341, 352
Mph1103I ATGCAT 1 cut(s) 120
MroXI GAANNNNTTC 1 cut(s) 384
MseI TTAA 1 cut(s) 409
MspA1I CMGCKG 1 cut(s) 23
MwoI GCNNNNNNNGC 2 cut(s) 233, 242
NdeI CATATG 1 cut(s) 251
NdeII GATC 2 cut(s) 157, 262
NlaIII CATG 5 cut(s) 58, 73, 118, 122, 147
NlaIV GGNNCC 1 cut(s) 353
NsiI ATGCAT 1 cut(s) 120
NspI RCATGY 2 cut(s) 118, 147
PdmI GAANNNNTTC 1 cut(s) 384
PfeI GAWTC 1 cut(s) 129
PflMI CCANNNNNTGG 1 cut(s) 361
PkrI GCNGC 1 cut(s) 25
PspN4I GGNNCC 1 cut(s) 353
SaqAI TTAA 1 cut(s) 409
SatI GCNGC 1 cut(s) 24
Sau3AI GATC 2 cut(s) 157, 262
SduI GDGCHC 1 cut(s) 323
SetI ASST 2 cut(s) 12, 393
SfaNI GCATC 1 cut(s) 107
Sse9I AATT 2 cut(s) 313, 328
SsiI CCGC 2 cut(s) 23, 60
SspMI CTAG 1 cut(s) 345
TaaI ACNGT 1 cut(s) 155
TaqI TCGA 1 cut(s) 378
TasI AATT 2 cut(s) 313, 328
TauI GCSGC 1 cut(s) 26
TfiI GAWTC 1 cut(s) 129
Tru1I TTAA 1 cut(s) 409
Tru9I TTAA 1 cut(s) 409
TspDTI ATGAA 1 cut(s) 22
Van91I CCANNNNNTGG 1 cut(s) 361
XbaI TCTAGA 1 cut(s) 344
XceI RCATGY 2 cut(s) 118, 147
XmnI GAANNNNTTC 1 cut(s) 384
XspI CTAG 1 cut(s) 345
Zsp2I ATGCAT 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.