Rroxscaffold_2G00085190

expansin-A23-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001146
Physical Location & Seq
Forward (+)
7542609 .. 7543597
989 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00085190.1

Sequence Viewer

Length: 756 bp
ATGGCTAAGTTCCAAAACTTACTCGCATGGGCAGTGCTCATCATCAGCACTCTTTTGGTGCATGCCATGGGCAATGATGCTTGGGAGGATGGAGCCGCAACGTTTTATGGTGACATGAGCGGAGGAGAAACCATGCAGGGGGCTTGTGGATATGGTGATCTCTTCCAACAAGGCTATGGCCTCGAGACAGCAGCTCTAAGCACAGCACTGTTTAATAATGGACTAACTTGTGGTGCTTGTTTTGAGATCATGTGTGTCAATGATCCACAATGGTGCATACCAAATGCTGGTACCATCAAAATCACTGCGACAAACTTTTGTCCTCCAAATTATGATCCACATTTTTATCCCTGGTGCAATCCCCCAAGGAAACACTTTGACTTGGCCATGCCCATGTTCGTAAAAATTGCTATCGCCAAAGCTGGGATAATTCCAGTAAAGTTTCGTAGAGTCCGATGTGCTAAGACAGGAGGGGTTAGGTTTGAGCTTAAAGGAAACCCCTACTGGATTACTGCAACCATCTTGAATGTTGGTGGTGCTGGTGATGTTACGGCCGTGAAGATTAAAGGGTCTAATTCAGATTGGCTTCAAATGCAACGCAACTGGGGACAAGTGTGGCAGATCGGAGTTAACTTAGTCGGACAAGGCTTATCGTTTCAAGTCACTACTAGTGATGGGAAGACGGTTGAGCTTGATAATGTTGCACCAGGAAATTGGCAATTTGGTCAAACCTATGAAGGGAAAAGCAATTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

251

Amino Acids

27.36

Weight (kDa)

6.8

Isoelectric Point (pI)

17.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXPB1_D1 PF22514 34 - 153 4.6e-06 EXPB1-like domain 1
DPBB_1 PF03330 63 - 148 2.9e-19 Lytic transglycolase
Expansin_C PF01357 159 - 236 6.2e-28 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 290
AccB1I GGYRCC 1 cut(s) 290
AccB7I CCANNNNNTGG 1 cut(s) 287
AccBSI CCGCTC 1 cut(s) 120
AciI CCGC 2 cut(s) 96, 120
AclI AACGTT 1 cut(s) 101
AclWI GGATC 2 cut(s) 257, 329
AcoI YGGCCR 2 cut(s) 384, 552
AfaI GTAC 1 cut(s) 292
AfiI CCNNNNNNNGG 4 cut(s) 138, 287, 423, 738
AgsI TTSAA 3 cut(s) 526, 590, 659
AhlI ACTAGT 1 cut(s) 668
AjnI CCWGG 2 cut(s) 350, 706
AleI CACNNNNGTG 1 cut(s) 271
AluBI AGCT 4 cut(s) 194, 422, 487, 691
AluI AGCT 4 cut(s) 194, 422, 487, 691
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 1 cut(s) 179
AlwI GGATC 2 cut(s) 257, 329
Ama87I CYCGRG 1 cut(s) 182
AoxI GGCC 3 cut(s) 178, 384, 552
ApeKI GCWGC 1 cut(s) 191
Asp718I GGTACC 1 cut(s) 290
AsuHPI GGTGA 3 cut(s) 122, 167, 554
AvaI CYCGRG 1 cut(s) 182
BalI TGGCCA 1 cut(s) 386
BanI GGYRCC 1 cut(s) 290
BbsI GAAGAC 1 cut(s) 686
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 1 cut(s) 203
BccI CCATC 4 cut(s) 83, 302, 527, 668
BceAI ACGGC 2 cut(s) 539, 567
BciT130I CCWGG 2 cut(s) 352, 708
BcoDI GTCTC 1 cut(s) 179
BcuI ACTAGT 1 cut(s) 668
BfaI CTAG 1 cut(s) 669
BisI GCNGC 2 cut(s) 96, 192
BlsI GCNGC 2 cut(s) 97, 193
Bme1390I CCNGG 2 cut(s) 352, 708
BmeT110I CYCGRG 1 cut(s) 182
BmiI GGNNCC 2 cut(s) 94, 292
BmrFI CCNGG 2 cut(s) 352, 708
BmrI ACTGGG 1 cut(s) 613
BmsI GCATC 1 cut(s) 67
BmuI ACTGGG 1 cut(s) 613
BpiI GAAGAC 1 cut(s) 686
BsaJI CCNNGG 3 cut(s) 66, 350, 365
Bsc4I CCNNNNNNNGG 4 cut(s) 138, 287, 423, 738
Bse1I ACTGG 3 cut(s) 434, 509, 608
Bse3DI GCAATG 1 cut(s) 79
BseBI CCWGG 2 cut(s) 352, 708
BseDI CCNNGG 3 cut(s) 66, 350, 365
BseGI GGATG 1 cut(s) 94
BseLI CCNNNNNNNGG 4 cut(s) 138, 287, 423, 738
BseMI GCAATG 1 cut(s) 79
BseNI ACTGG 3 cut(s) 434, 509, 608
BseRI GAGGAG 1 cut(s) 138
BseX3I CGGCCG 1 cut(s) 552
BseXI GCAGC 1 cut(s) 203
BseYI CCCAGC 1 cut(s) 422
Bsh1285I CGRYCG 1 cut(s) 555
BshFI GGCC 3 cut(s) 180, 386, 554
BshNI GGYRCC 1 cut(s) 290
BsiEI CGRYCG 1 cut(s) 555
BsiHKAI GWGCWC 1 cut(s) 39
BsiHKCI CYCGRG 1 cut(s) 182
BslFI GGGAC 1 cut(s) 621
BslI CCNNNNNNNGG 4 cut(s) 138, 287, 423, 738
BsmAI GTCTC 1 cut(s) 179
BsmFI GGGAC 1 cut(s) 621
BsnI GGCC 3 cut(s) 180, 386, 554
BsoBI CYCGRG 1 cut(s) 182
Bsp1286I GDGCHC 1 cut(s) 39
Bsp143I GATC 5 cut(s) 157, 246, 262, 334, 621
Bsp19I CCATGG 1 cut(s) 66
BspACI CCGC 2 cut(s) 96, 120
BspANI GGCC 3 cut(s) 180, 386, 554
BspLI GGNNCC 2 cut(s) 94, 292
BspPI GGATC 2 cut(s) 257, 329
BspT107I GGYRCC 1 cut(s) 290
BsrBI CCGCTC 1 cut(s) 120
BsrDI GCAATG 1 cut(s) 79
BsrI ACTGG 3 cut(s) 434, 509, 608
BssECI CCNNGG 3 cut(s) 66, 350, 365
BssMI GATC 5 cut(s) 157, 246, 262, 334, 621
BssT1I CCWWGG 2 cut(s) 66, 365
Bst2UI CCWGG 2 cut(s) 352, 708
Bst4CI ACNGT 2 cut(s) 210, 685
Bst6I CTCTTC 1 cut(s) 167
BstC8I GCNNGC 1 cut(s) 63
BstDEI CTNAG 4 cut(s) 6, 197, 462, 634
BstDSI CCRYGG 1 cut(s) 66
BstF5I GGATG 1 cut(s) 94
BstKTI GATC 5 cut(s) 160, 249, 265, 337, 624
BstMAI GTCTC 1 cut(s) 179
BstMBI GATC 5 cut(s) 157, 246, 262, 334, 621
BstMCI CGRYCG 1 cut(s) 555
BstMWI GCNNNNNNNGC 1 cut(s) 592
BstNI CCWGG 2 cut(s) 352, 708
BstNSI RCATGY 1 cut(s) 65
BstSCI CCNGG 2 cut(s) 350, 706
BstV1I GCAGC 1 cut(s) 203
BstV2I GAAGAC 1 cut(s) 686
BstXI CCANNNNNNTGG 1 cut(s) 714
BstZI CGGCCG 1 cut(s) 552
BsuRI GGCC 3 cut(s) 180, 386, 554
BtgI CCRYGG 1 cut(s) 66
BtsCI GGATG 1 cut(s) 94
BtsI GCAGTG 2 cut(s) 39, 303
BtsIMutI CAGTG 3 cut(s) 39, 206, 303
Cac8I GCNNGC 1 cut(s) 63
Csp6I GTAC 1 cut(s) 291
CviAII CATG 8 cut(s) 27, 62, 67, 115, 133, 250, 388, 394
CviQI GTAC 1 cut(s) 291
DdeI CTNAG 4 cut(s) 6, 197, 462, 634
DpnI GATC 5 cut(s) 159, 248, 264, 336, 623
DpnII GATC 5 cut(s) 157, 246, 262, 334, 621
EaeI YGGCCR 2 cut(s) 384, 552
EagI CGGCCG 1 cut(s) 552
Eam1104I CTCTTC 1 cut(s) 167
EarI CTCTTC 1 cut(s) 167
EclXI CGGCCG 1 cut(s) 552
Eco130I CCWWGG 2 cut(s) 66, 365
Eco52I CGGCCG 1 cut(s) 552
Eco88I CYCGRG 1 cut(s) 182
EcoRII CCWGG 2 cut(s) 350, 706
EcoT14I CCWWGG 2 cut(s) 66, 365
ErhI CCWWGG 2 cut(s) 66, 365
FaeI CATG 8 cut(s) 30, 65, 70, 118, 136, 253, 391, 397
FaqI GGGAC 1 cut(s) 621
FatI CATG 8 cut(s) 26, 61, 66, 114, 132, 249, 387, 393
Fnu4HI GCNGC 2 cut(s) 96, 192
FokI GGATG 1 cut(s) 101
Fsp4HI GCNGC 2 cut(s) 96, 192
FspBI CTAG 1 cut(s) 669
GluI GCNGC 2 cut(s) 96, 192
GsaI CCCAGC 1 cut(s) 426
HaeIII GGCC 3 cut(s) 180, 386, 554
Hin1II CATG 8 cut(s) 30, 65, 70, 118, 136, 253, 391, 397
HincII GTYRAC 1 cut(s) 631
HindII GTYRAC 1 cut(s) 631
HinfI GANTC 1 cut(s) 450
HpaI GTTAAC 1 cut(s) 631
HphI GGTGA 3 cut(s) 122, 167, 554
Hpy166II GTNNAC 1 cut(s) 631
Hpy188I TCNGA 4 cut(s) 455, 580, 626, 641
Hpy188III TCNNGA 2 cut(s) 184, 523
Hpy8I GTNNAC 1 cut(s) 631
HpyAV CCTTC 1 cut(s) 731
HpyCH4III ACNGT 2 cut(s) 210, 685
HpyCH4IV ACGT 1 cut(s) 101
HpyCH4V TGCA 7 cut(s) 61, 136, 276, 357, 515, 595, 704
HpyF10VI GCNNNNNNNGC 1 cut(s) 592
HpyF3I CTNAG 4 cut(s) 6, 197, 462, 634
HpySE526I ACGT 1 cut(s) 101
Hsp92II CATG 8 cut(s) 30, 65, 70, 118, 136, 253, 391, 397
KpnI GGTACC 1 cut(s) 294
KspAI GTTAAC 1 cut(s) 631
Kzo9I GATC 5 cut(s) 157, 246, 262, 334, 621
LmnI GCTCC 1 cut(s) 92
Lsp1109I GCAGC 1 cut(s) 203
LweI GCATC 1 cut(s) 67
MaeI CTAG 1 cut(s) 669
MaeII ACGT 1 cut(s) 101
MaeIII GTNAC 3 cut(s) 110, 547, 661
MalI GATC 5 cut(s) 159, 248, 264, 336, 623
MbiI CCGCTC 1 cut(s) 120
MboI GATC 5 cut(s) 157, 246, 262, 334, 621
MboII GAAGA 3 cut(s) 154, 571, 691
MhlI GDGCHC 1 cut(s) 39
MlsI TGGCCA 1 cut(s) 386
MluCI AATT 7 cut(s) 328, 405, 429, 574, 712, 719, 748
MluNI TGGCCA 1 cut(s) 386
MlyI GAGTC 1 cut(s) 459
MmeI TCCRAC 2 cut(s) 190, 619
MnlI CCTC 5 cut(s) 79, 116, 191, 333, 464
Mox20I TGGCCA 1 cut(s) 386
MscI TGGCCA 1 cut(s) 386
MseI TTAA 4 cut(s) 213, 489, 564, 630
MslI CAYNNNNRTG 2 cut(s) 271, 392
Msp20I TGGCCA 1 cut(s) 386
MspR9I CCNGG 2 cut(s) 352, 708
MvaI CCWGG 2 cut(s) 352, 708
MwoI GCNNNNNNNGC 1 cut(s) 592
NcoI CCATGG 1 cut(s) 66
NdeII GATC 5 cut(s) 157, 246, 262, 334, 621
NlaIII CATG 8 cut(s) 30, 65, 70, 118, 136, 253, 391, 397
NlaIV GGNNCC 2 cut(s) 94, 292
NmuCI GTSAC 2 cut(s) 110, 661
NspI RCATGY 1 cut(s) 65
OliI CACNNNNGTG 1 cut(s) 271
PaeI GCATGC 1 cut(s) 65
PaeR7I CTCGAG 1 cut(s) 182
PcsI WCGNNNNNNNCGW 1 cut(s) 451
PflMI CCANNNNNTGG 1 cut(s) 287
PkrI GCNGC 2 cut(s) 97, 193
PleI GAGTC 1 cut(s) 458
PpsI GAGTC 1 cut(s) 458
Psp1406I AACGTT 1 cut(s) 101
Psp6I CCWGG 2 cut(s) 350, 706
PspFI CCCAGC 1 cut(s) 422
PspGI CCWGG 2 cut(s) 350, 706
PspN4I GGNNCC 2 cut(s) 94, 292
RsaI GTAC 1 cut(s) 292
RsaNI GTAC 1 cut(s) 291
RseI CAYNNNNRTG 2 cut(s) 271, 392
SaqAI TTAA 4 cut(s) 213, 489, 564, 630
SatI GCNGC 2 cut(s) 96, 192
Sau3AI GATC 5 cut(s) 157, 246, 262, 334, 621
SchI GAGTC 1 cut(s) 459
ScrFI CCNGG 2 cut(s) 352, 708
SduI GDGCHC 1 cut(s) 39
SetI ASST 7 cut(s) 104, 196, 424, 482, 489, 693, 734
SfaNI GCATC 1 cut(s) 67
Sfr274I CTCGAG 1 cut(s) 182
SlaI CTCGAG 1 cut(s) 182
SmiMI CAYNNNNRTG 2 cut(s) 271, 392
SmlI CTYRAG 1 cut(s) 182
SmoI CTYRAG 1 cut(s) 182
SpeI ACTAGT 1 cut(s) 668
SphI GCATGC 1 cut(s) 65
Sse9I AATT 7 cut(s) 328, 405, 429, 574, 712, 719, 748
SsiI CCGC 2 cut(s) 96, 120
SspMI CTAG 1 cut(s) 669
StyD4I CCNGG 2 cut(s) 350, 706
StyI CCWWGG 2 cut(s) 66, 365
TaaI ACNGT 2 cut(s) 210, 685
TaiI ACGT 1 cut(s) 104
TaqI TCGA 1 cut(s) 183
TasI AATT 7 cut(s) 328, 405, 429, 574, 712, 719, 748
TauI GCSGC 1 cut(s) 98
Tru1I TTAA 4 cut(s) 213, 489, 564, 630
Tru9I TTAA 4 cut(s) 213, 489, 564, 630
TscAI CASTG 3 cut(s) 39, 213, 310
TseFI GTSAC 2 cut(s) 110, 661
TseI GCWGC 1 cut(s) 191
Tsp45I GTSAC 2 cut(s) 110, 661
TspDTI ATGAA 1 cut(s) 750
TspRI CASTG 3 cut(s) 39, 213, 310
Van91I CCANNNNNTGG 1 cut(s) 287
XceI RCATGY 1 cut(s) 65
XcmI CCANNNNNNNNNTGG 1 cut(s) 173
XhoI CTCGAG 1 cut(s) 182
XspI CTAG 1 cut(s) 669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.