RchiOBHm_Chr5g0061541

expansin-A23-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001146
Physical Location & Seq
Forward (+)
67049657 .. 67050391
735 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33791

Sequence Viewer

Length: 498 bp
ATGGTGAAGTATTTGCTGACAACAGCGTCCGTGTTCATCTCTCTTTTGGTTCCGGGCATGCGCGGTGACGACATGACGACTTGGTTTACTGGCGCACACGCATCGTTGGGGAATGTGGATATGGTGAGCAATAAACGAGAATATAACCTGCAGACAGCAGCTCTCAGCAAAGCACTATTCAACGACGGCCTTGCTTGTGGCGCCTGTTTCGCCATCATGTGTGTCGATGATACGAAAGGGTGCAAACCCAGTGCTGGTTCTGCCGTAGTTACTGCAACCGATTATTGCCCCCGAATTTATACTAGGCCTCCCGATGGAACCAACAACTGGTGCAACCCGCCATTGAAACACTTCAAGTTAAGCATGCCAATGTTCACAAGAATCGCAGAAGAAAAGGTTGGGGTGGTACCGGTTGTATATCGCAGAGTCCCGTACTCCCGTGTGCTAAGGAGGGAGGGGTTAAGTTCCAATTATTCAAGGGAAAGTACTCTAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

165

Amino Acids

18.18

Weight (kDa)

9.1

Isoelectric Point (pI)

29.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXPB1_D1 PF22514 49 - 112 9.1e-06 EXPB1-like domain 1
DPBB_1 PF03330 52 - 140 2.8e-17 Lytic transglycolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 25
Acc36I ACCTGC 1 cut(s) 156
Acc65I GGTACC 1 cut(s) 406
AccB1I GGYRCC 2 cut(s) 200, 406
AccB7I CCANNNNNTGG 1 cut(s) 327
AccII CGCG 1 cut(s) 63
AciI CCGC 2 cut(s) 63, 338
AcsI RAATTY 1 cut(s) 294
AcyI GRCGYC 1 cut(s) 201
AfaI GTAC 3 cut(s) 408, 434, 487
AfiI CCNNNNNNNGG 3 cut(s) 254, 314, 327
AgeI ACCGGT 1 cut(s) 409
AgsI TTSAA 4 cut(s) 181, 346, 355, 477
AjuI GAANNNNNNNTTGG 2 cut(s) 381, 413
AluBI AGCT 1 cut(s) 161
AluI AGCT 1 cut(s) 161
AoxI GGCC 2 cut(s) 187, 305
ApeKI GCWGC 1 cut(s) 158
ApoI RAATTY 1 cut(s) 294
AsiGI ACCGGT 1 cut(s) 409
Asp700I GAANNNNTTC 1 cut(s) 350
Asp718I GGTACC 1 cut(s) 406
AspLEI GCGC 3 cut(s) 63, 95, 203
AsuC2I CCSGG 1 cut(s) 54
AsuHPI GGTGA 3 cut(s) 16, 77, 136
BanI GGYRCC 2 cut(s) 200, 406
BbvI GCAGC 1 cut(s) 170
BccI CCATC 2 cut(s) 221, 308
BceAI ACGGC 2 cut(s) 202, 248
BcgI CGANNNNNNTGC 4 cut(s) 84, 118, 173, 207
BcnI CCSGG 1 cut(s) 54
BfaI CTAG 1 cut(s) 303
BfmI CTRYAG 1 cut(s) 149
BfoI RGCGCY 1 cut(s) 204
BfuAI ACCTGC 1 cut(s) 156
BisI GCNGC 1 cut(s) 159
BlsI GCNGC 1 cut(s) 160
BmcAI AGTACT 1 cut(s) 487
Bme1390I CCNGG 1 cut(s) 54
BmiI GGNNCC 4 cut(s) 51, 202, 319, 408
BmrFI CCNGG 1 cut(s) 54
BmrI ACTGGG 1 cut(s) 243
BmsI GCATC 1 cut(s) 110
BmuI ACTGGG 1 cut(s) 243
Bpu10I CCTNAGC 1 cut(s) 446
BpuMI CCSGG 1 cut(s) 54
BsaHI GRCGYC 1 cut(s) 201
BsaWI WCCGGW 1 cut(s) 409
BsaXI ACNNNNNCTCC 2 cut(s) 292, 322
Bsc4I CCNNNNNNNGG 3 cut(s) 254, 314, 327
Bse118I RCCGGY 1 cut(s) 409
Bse1I ACTGG 3 cut(s) 94, 249, 332
BseLI CCNNNNNNNGG 3 cut(s) 254, 314, 327
BseMII CTCAG 1 cut(s) 178
BseNI ACTGG 3 cut(s) 94, 249, 332
BseXI GCAGC 1 cut(s) 170
Bsh1236I CGCG 1 cut(s) 63
BshFI GGCC 2 cut(s) 189, 307
BshNI GGYRCC 2 cut(s) 200, 406
BshTI ACCGGT 1 cut(s) 409
BsiSI CCGG 2 cut(s) 53, 410
BslFI GGGAC 1 cut(s) 413
BslI CCNNNNNNNGG 3 cut(s) 254, 314, 327
BsmFI GGGAC 1 cut(s) 413
BsnI GGCC 2 cut(s) 189, 307
BspACI CCGC 2 cut(s) 63, 338
BspANI GGCC 2 cut(s) 189, 307
BspCNI CTCAG 1 cut(s) 177
BspFNI CGCG 1 cut(s) 63
BspLI GGNNCC 4 cut(s) 51, 202, 319, 408
BspMAI CTGCAG 1 cut(s) 153
BspMI ACCTGC 1 cut(s) 156
BspT107I GGYRCC 2 cut(s) 200, 406
BsrFI RCCGGY 1 cut(s) 409
BsrI ACTGG 3 cut(s) 94, 249, 332
BssAI RCCGGY 1 cut(s) 409
BssNI GRCGYC 1 cut(s) 201
BstACI GRCGYC 1 cut(s) 201
BstC8I GCNNGC 2 cut(s) 59, 365
BstDEI CTNAG 2 cut(s) 164, 446
BstFNI CGCG 1 cut(s) 63
BstH2I RGCGCY 1 cut(s) 204
BstHHI GCGC 3 cut(s) 63, 95, 203
BstMWI GCNNNNNNNGC 3 cut(s) 200, 209, 260
BstNSI RCATGY 2 cut(s) 61, 367
BstSCI CCNGG 1 cut(s) 52
BstSFI CTRYAG 1 cut(s) 149
BstUI CGCG 1 cut(s) 63
BstV1I GCAGC 1 cut(s) 170
BsuRI GGCC 2 cut(s) 189, 307
BtsIMutI CAGTG 1 cut(s) 256
BveI ACCTGC 1 cut(s) 156
Cac8I GCNNGC 2 cut(s) 59, 365
CfoI GCGC 3 cut(s) 63, 95, 203
Cfr10I RCCGGY 1 cut(s) 409
CseI GACGC 1 cut(s) 15
Csp6I GTAC 3 cut(s) 407, 433, 486
CspAI ACCGGT 1 cut(s) 409
CviAII CATG 4 cut(s) 58, 73, 217, 364
CviJI RGCY 3 cut(s) 161, 189, 307
CviKI_1 RGCY 3 cut(s) 161, 189, 307
CviQI GTAC 3 cut(s) 407, 433, 486
DdeI CTNAG 2 cut(s) 164, 446
DinI GGCGCC 1 cut(s) 202
DrdI GACNNNNNNGTC 1 cut(s) 25
DseDI GACNNNNNNGTC 1 cut(s) 25
Eco147I AGGCCT 1 cut(s) 307
EgeI GGCGCC 1 cut(s) 202
EheI GGCGCC 1 cut(s) 202
FaeI CATG 4 cut(s) 61, 76, 220, 367
FaiI YATR 8 cut(s) 59, 74, 122, 144, 218, 300, 365, 418
FaqI GGGAC 1 cut(s) 413
FatI CATG 4 cut(s) 57, 72, 216, 363
FauI CCCGC 1 cut(s) 345
Fnu4HI GCNGC 1 cut(s) 159
Fsp4HI GCNGC 1 cut(s) 159
FspBI CTAG 1 cut(s) 303
GlaI GCGC 3 cut(s) 62, 94, 202
GluI GCNGC 1 cut(s) 159
HaeII RGCGCY 1 cut(s) 204
HaeIII GGCC 2 cut(s) 189, 307
HapII CCGG 2 cut(s) 53, 410
HgaI GACGC 1 cut(s) 15
HhaI GCGC 3 cut(s) 63, 95, 203
Hin1I GRCGYC 1 cut(s) 201
Hin1II CATG 4 cut(s) 61, 76, 220, 367
Hin6I GCGC 3 cut(s) 61, 93, 201
HinP1I GCGC 3 cut(s) 61, 93, 201
HinfI GANTC 2 cut(s) 381, 426
HpaII CCGG 2 cut(s) 53, 410
HphI GGTGA 3 cut(s) 16, 77, 136
Hpy166II GTNNAC 2 cut(s) 87, 375
Hpy188III TCNNGA 1 cut(s) 311
Hpy8I GTNNAC 2 cut(s) 87, 375
Hpy99I CGWCG 1 cut(s) 188
HpyCH4V TGCA 4 cut(s) 151, 243, 275, 333
HpyF10VI GCNNNNNNNGC 3 cut(s) 200, 209, 260
HpyF3I CTNAG 2 cut(s) 164, 446
Hsp92I GRCGYC 1 cut(s) 201
Hsp92II CATG 4 cut(s) 61, 76, 220, 367
HspAI GCGC 3 cut(s) 61, 93, 201
KasI GGCGCC 1 cut(s) 200
KpnI GGTACC 1 cut(s) 410
LpnPI CCDG 8 cut(s) 66, 75, 161, 217, 240, 262, 313, 423
Lsp1109I GCAGC 1 cut(s) 170
LweI GCATC 1 cut(s) 110
MaeI CTAG 1 cut(s) 303
MaeIII GTNAC 2 cut(s) 65, 268
MboII GAAGA 1 cut(s) 401
MluCI AATT 3 cut(s) 294, 469, 492
Mly113I GGCGCC 1 cut(s) 201
MlyI GAGTC 1 cut(s) 435
MnlI CCTC 3 cut(s) 318, 444, 448
MroXI GAANNNNTTC 1 cut(s) 350
MseI TTAA 2 cut(s) 359, 461
MslI CAYNNNNRTG 1 cut(s) 368
MspI CCGG 2 cut(s) 53, 410
MspR9I CCNGG 1 cut(s) 54
MvnI CGCG 1 cut(s) 63
MwoI GCNNNNNNNGC 3 cut(s) 200, 209, 260
NarI GGCGCC 1 cut(s) 201
NciI CCSGG 1 cut(s) 54
NlaIII CATG 4 cut(s) 61, 76, 220, 367
NlaIV GGNNCC 4 cut(s) 51, 202, 319, 408
NmuCI GTSAC 1 cut(s) 65
NspI RCATGY 2 cut(s) 61, 367
PaeI GCATGC 2 cut(s) 61, 367
PceI AGGCCT 1 cut(s) 307
PdmI GAANNNNTTC 1 cut(s) 350
PfeI GAWTC 1 cut(s) 381
PflMI CCANNNNNTGG 1 cut(s) 327
PinAI ACCGGT 1 cut(s) 409
PkrI GCNGC 1 cut(s) 160
PleI GAGTC 1 cut(s) 434
PluTI GGCGCC 1 cut(s) 204
PpsI GAGTC 1 cut(s) 434
PspN4I GGNNCC 4 cut(s) 51, 202, 319, 408
PstI CTGCAG 1 cut(s) 153
RsaI GTAC 3 cut(s) 408, 434, 487
RsaNI GTAC 3 cut(s) 407, 433, 486
RseI CAYNNNNRTG 1 cut(s) 368
SaqAI TTAA 2 cut(s) 359, 461
SatI GCNGC 1 cut(s) 159
ScaI AGTACT 1 cut(s) 487
SchI GAGTC 1 cut(s) 435
ScrFI CCNGG 1 cut(s) 54
SetI ASST 3 cut(s) 150, 163, 399
SfaNI GCATC 1 cut(s) 110
SfcI CTRYAG 1 cut(s) 149
SfoI GGCGCC 1 cut(s) 202
SmiMI CAYNNNNRTG 1 cut(s) 368
SphI GCATGC 2 cut(s) 61, 367
Sse9I AATT 3 cut(s) 294, 469, 492
SseBI AGGCCT 1 cut(s) 307
SsiI CCGC 2 cut(s) 63, 338
SspDI GGCGCC 1 cut(s) 200
SspMI CTAG 1 cut(s) 303
StuI AGGCCT 1 cut(s) 307
StyD4I CCNGG 1 cut(s) 52
TaqI TCGA 1 cut(s) 225
TasI AATT 3 cut(s) 294, 469, 492
TatI WGTACW 1 cut(s) 485
TfiI GAWTC 1 cut(s) 381
Tru1I TTAA 2 cut(s) 359, 461
Tru9I TTAA 2 cut(s) 359, 461
TscAI CASTG 1 cut(s) 256
TseFI GTSAC 1 cut(s) 65
TseI GCWGC 1 cut(s) 158
Tsp45I GTSAC 1 cut(s) 65
TspDTI ATGAA 1 cut(s) 25
TspGWI ACGGA 1 cut(s) 19
TspRI CASTG 1 cut(s) 256
Van91I CCANNNNNTGG 1 cut(s) 327
XapI RAATTY 1 cut(s) 294
XceI RCATGY 2 cut(s) 61, 367
XmnI GAANNNNTTC 1 cut(s) 350
XspI CTAG 1 cut(s) 303
ZrmI AGTACT 1 cut(s) 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.