RLG00000021586

expansin-A23-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001146
Physical Location & Seq
Reverse (-)
76607167 .. 76608157
991 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021586

Sequence Viewer

Length: 756 bp
ATGGCTAAGTTCCAAAACTTACTCGCATGGGCAGTGCTCATCATCAGCACTCTTTTGGTGCATGCCATGGGCGATGATGCTTGGGAGGATGGAGCCGCAACGTTTTATGGTGACATGAGCGGAGGAGAAACCATGCAGGGGGCTTGTGGATATGGTGATCTCTTCCAACAAGGCTATGGCCTCGAGACAGCAGCTCTAAGCACAGCACTGTTTAACAATGGACTAACTTGTGGTGCTTGTTTTGAGATCATGTGTGTCAATGATCCACAATGGTGCATACCAAATGCAGGTACCATCAAAATCACTGCGACAAACTTTTGTCCTCCAAATTATGATCCACATTTTTATCCCTGGTGCAACCCCCCAAGAAAACACTTTGACTTGGCCATGCCCATGTTCGTAAAAATTGCCATCACCAAAGCTGGGATAATTCCAGTAAAGTTTCGTAGAGTTCCATGTGCTAAGACAGGAGGGGTTAGGTTTGAGCTTAAAGGAAACCCCTACTGGATTACTGCAACCATCTTGAATGTTGGTGGTGCTGGTGATGTTACGGCCGTGAAGATTAAAGGGTCTAATTCCGATTGGCTTCAAATGCAACGCAACTGGGGACAAGTTTGGCAGATCGGAGTTAACTTAGTCGGACAAGGCTTATCGTTTCAAGTCACTACTAGTGATGGGAAGATGGTTGAGCTTGATAATATTGCACCAGGAAATTGGCAATTTGGTCAAACCTATGAAGGGAAAAGCAATTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

252

Amino Acids

27.41

Weight (kDa)

5.89

Isoelectric Point (pI)

20.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXPB1_D1 PF22514 34 - 154 2.5e-06 EXPB1-like domain 1
DPBB_1 PF03330 63 - 148 1.3e-19 Lytic transglycolase
Expansin_C PF01357 159 - 236 6.6e-27 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 278
Acc65I GGTACC 1 cut(s) 290
AccB1I GGYRCC 1 cut(s) 290
AccBSI CCGCTC 1 cut(s) 120
AciI CCGC 2 cut(s) 96, 120
AclI AACGTT 1 cut(s) 101
AclWI GGATC 2 cut(s) 257, 329
AcoI YGGCCR 2 cut(s) 384, 552
AfaI GTAC 1 cut(s) 292
AfiI CCNNNNNNNGG 4 cut(s) 138, 287, 423, 738
AgsI TTSAA 3 cut(s) 526, 590, 659
AhlI ACTAGT 1 cut(s) 668
AjnI CCWGG 2 cut(s) 350, 706
AleI CACNNNNGTG 1 cut(s) 271
AluBI AGCT 4 cut(s) 194, 422, 487, 691
AluI AGCT 4 cut(s) 194, 422, 487, 691
Alw21I GWGCWC 1 cut(s) 39
Alw26I GTCTC 1 cut(s) 179
AlwI GGATC 2 cut(s) 257, 329
Ama87I CYCGRG 1 cut(s) 182
AoxI GGCC 3 cut(s) 178, 384, 552
ApeKI GCWGC 1 cut(s) 191
Asp718I GGTACC 1 cut(s) 290
AsuHPI GGTGA 4 cut(s) 122, 167, 406, 554
AvaI CYCGRG 1 cut(s) 182
BalI TGGCCA 1 cut(s) 386
BanI GGYRCC 1 cut(s) 290
Bbv12I GWGCWC 1 cut(s) 39
BbvI GCAGC 1 cut(s) 203
BccI CCATC 6 cut(s) 83, 302, 419, 527, 668, 676
BceAI ACGGC 2 cut(s) 539, 567
BciT130I CCWGG 2 cut(s) 352, 708
BcoDI GTCTC 1 cut(s) 179
BcuI ACTAGT 1 cut(s) 668
BfaI CTAG 1 cut(s) 669
BfuAI ACCTGC 1 cut(s) 278
BisI GCNGC 2 cut(s) 96, 192
BlsI GCNGC 2 cut(s) 97, 193
Bme1390I CCNGG 2 cut(s) 352, 708
BmeT110I CYCGRG 1 cut(s) 182
BmiI GGNNCC 2 cut(s) 94, 292
BmrFI CCNGG 2 cut(s) 352, 708
BmrI ACTGGG 1 cut(s) 613
BmsI GCATC 1 cut(s) 67
BmuI ACTGGG 1 cut(s) 613
BsaJI CCNNGG 2 cut(s) 66, 350
Bsc4I CCNNNNNNNGG 4 cut(s) 138, 287, 423, 738
Bse1I ACTGG 3 cut(s) 434, 509, 608
BseBI CCWGG 2 cut(s) 352, 708
BseDI CCNNGG 2 cut(s) 66, 350
BseGI GGATG 1 cut(s) 94
BseLI CCNNNNNNNGG 4 cut(s) 138, 287, 423, 738
BseNI ACTGG 3 cut(s) 434, 509, 608
BseRI GAGGAG 1 cut(s) 138
BseX3I CGGCCG 1 cut(s) 552
BseXI GCAGC 1 cut(s) 203
BseYI CCCAGC 1 cut(s) 422
Bsh1285I CGRYCG 1 cut(s) 555
BshFI GGCC 3 cut(s) 180, 386, 554
BshNI GGYRCC 1 cut(s) 290
BsiEI CGRYCG 1 cut(s) 555
BsiHKAI GWGCWC 1 cut(s) 39
BsiHKCI CYCGRG 1 cut(s) 182
BslFI GGGAC 1 cut(s) 621
BslI CCNNNNNNNGG 4 cut(s) 138, 287, 423, 738
BsmAI GTCTC 1 cut(s) 179
BsmFI GGGAC 1 cut(s) 621
BsnI GGCC 3 cut(s) 180, 386, 554
BsoBI CYCGRG 1 cut(s) 182
Bsp1286I GDGCHC 1 cut(s) 39
Bsp143I GATC 5 cut(s) 157, 246, 262, 334, 621
Bsp19I CCATGG 1 cut(s) 66
BspACI CCGC 2 cut(s) 96, 120
BspANI GGCC 3 cut(s) 180, 386, 554
BspLI GGNNCC 2 cut(s) 94, 292
BspMI ACCTGC 1 cut(s) 278
BspPI GGATC 2 cut(s) 257, 329
BspT107I GGYRCC 1 cut(s) 290
BsrBI CCGCTC 1 cut(s) 120
BsrI ACTGG 3 cut(s) 434, 509, 608
BssECI CCNNGG 2 cut(s) 66, 350
BssMI GATC 5 cut(s) 157, 246, 262, 334, 621
BssT1I CCWWGG 1 cut(s) 66
Bst2UI CCWGG 2 cut(s) 352, 708
Bst4CI ACNGT 1 cut(s) 210
Bst6I CTCTTC 1 cut(s) 167
BstC8I GCNNGC 1 cut(s) 63
BstDEI CTNAG 4 cut(s) 6, 197, 462, 634
BstDSI CCRYGG 1 cut(s) 66
BstF5I GGATG 1 cut(s) 94
BstKTI GATC 5 cut(s) 160, 249, 265, 337, 624
BstMAI GTCTC 1 cut(s) 179
BstMBI GATC 5 cut(s) 157, 246, 262, 334, 621
BstMCI CGRYCG 1 cut(s) 555
BstMWI GCNNNNNNNGC 1 cut(s) 592
BstNI CCWGG 2 cut(s) 352, 708
BstNSI RCATGY 1 cut(s) 65
BstSCI CCNGG 2 cut(s) 350, 706
BstV1I GCAGC 1 cut(s) 203
BstXI CCANNNNNNTGG 1 cut(s) 714
BstZI CGGCCG 1 cut(s) 552
BsuRI GGCC 3 cut(s) 180, 386, 554
BtgI CCRYGG 1 cut(s) 66
BtgZI GCGATG 1 cut(s) 87
BtsCI GGATG 1 cut(s) 94
BtsI GCAGTG 2 cut(s) 39, 303
BtsIMutI CAGTG 3 cut(s) 39, 206, 303
BveI ACCTGC 1 cut(s) 278
Cac8I GCNNGC 1 cut(s) 63
Csp6I GTAC 1 cut(s) 291
CviAII CATG 9 cut(s) 27, 62, 67, 115, 133, 250, 388, 394, 456
CviQI GTAC 1 cut(s) 291
DdeI CTNAG 4 cut(s) 6, 197, 462, 634
DpnI GATC 5 cut(s) 159, 248, 264, 336, 623
DpnII GATC 5 cut(s) 157, 246, 262, 334, 621
EaeI YGGCCR 2 cut(s) 384, 552
EagI CGGCCG 1 cut(s) 552
Eam1104I CTCTTC 1 cut(s) 167
EarI CTCTTC 1 cut(s) 167
EclXI CGGCCG 1 cut(s) 552
Eco130I CCWWGG 1 cut(s) 66
Eco52I CGGCCG 1 cut(s) 552
Eco88I CYCGRG 1 cut(s) 182
EcoRII CCWGG 2 cut(s) 350, 706
EcoT14I CCWWGG 1 cut(s) 66
ErhI CCWWGG 1 cut(s) 66
FaeI CATG 9 cut(s) 30, 65, 70, 118, 136, 253, 391, 397, 459
FaqI GGGAC 1 cut(s) 621
FatI CATG 9 cut(s) 26, 61, 66, 114, 132, 249, 387, 393, 455
Fnu4HI GCNGC 2 cut(s) 96, 192
FokI GGATG 1 cut(s) 101
Fsp4HI GCNGC 2 cut(s) 96, 192
FspBI CTAG 1 cut(s) 669
GluI GCNGC 2 cut(s) 96, 192
GsaI CCCAGC 1 cut(s) 426
HaeIII GGCC 3 cut(s) 180, 386, 554
Hin1II CATG 9 cut(s) 30, 65, 70, 118, 136, 253, 391, 397, 459
HincII GTYRAC 1 cut(s) 631
HindII GTYRAC 1 cut(s) 631
HpaI GTTAAC 1 cut(s) 631
HphI GGTGA 4 cut(s) 122, 167, 406, 554
Hpy166II GTNNAC 1 cut(s) 631
Hpy188I TCNGA 3 cut(s) 580, 626, 641
Hpy188III TCNNGA 2 cut(s) 184, 523
Hpy8I GTNNAC 1 cut(s) 631
HpyAV CCTTC 1 cut(s) 731
HpyCH4III ACNGT 1 cut(s) 210
HpyCH4IV ACGT 1 cut(s) 101
HpyCH4V TGCA 8 cut(s) 61, 136, 276, 287, 357, 515, 595, 704
HpyF10VI GCNNNNNNNGC 1 cut(s) 592
HpyF3I CTNAG 4 cut(s) 6, 197, 462, 634
HpySE526I ACGT 1 cut(s) 101
Hsp92II CATG 9 cut(s) 30, 65, 70, 118, 136, 253, 391, 397, 459
KpnI GGTACC 1 cut(s) 294
KspAI GTTAAC 1 cut(s) 631
Kzo9I GATC 5 cut(s) 157, 246, 262, 334, 621
LmnI GCTCC 1 cut(s) 92
Lsp1109I GCAGC 1 cut(s) 203
LweI GCATC 1 cut(s) 67
MaeI CTAG 1 cut(s) 669
MaeII ACGT 1 cut(s) 101
MaeIII GTNAC 3 cut(s) 110, 547, 661
MalI GATC 5 cut(s) 159, 248, 264, 336, 623
MbiI CCGCTC 1 cut(s) 120
MboI GATC 5 cut(s) 157, 246, 262, 334, 621
MboII GAAGA 3 cut(s) 154, 571, 691
MhlI GDGCHC 1 cut(s) 39
MlsI TGGCCA 1 cut(s) 386
MluCI AATT 7 cut(s) 328, 405, 429, 574, 712, 719, 748
MluNI TGGCCA 1 cut(s) 386
MmeI TCCRAC 2 cut(s) 190, 619
MnlI CCTC 5 cut(s) 79, 116, 191, 333, 464
Mox20I TGGCCA 1 cut(s) 386
MscI TGGCCA 1 cut(s) 386
MseI TTAA 4 cut(s) 213, 489, 564, 630
MslI CAYNNNNRTG 2 cut(s) 271, 392
Msp20I TGGCCA 1 cut(s) 386
MspR9I CCNGG 2 cut(s) 352, 708
MvaI CCWGG 2 cut(s) 352, 708
MwoI GCNNNNNNNGC 1 cut(s) 592
NcoI CCATGG 1 cut(s) 66
NdeII GATC 5 cut(s) 157, 246, 262, 334, 621
NlaIII CATG 9 cut(s) 30, 65, 70, 118, 136, 253, 391, 397, 459
NlaIV GGNNCC 2 cut(s) 94, 292
NmuCI GTSAC 2 cut(s) 110, 661
NspI RCATGY 1 cut(s) 65
OliI CACNNNNGTG 1 cut(s) 271
PaeI GCATGC 1 cut(s) 65
PaeR7I CTCGAG 1 cut(s) 182
PkrI GCNGC 2 cut(s) 97, 193
Psp1406I AACGTT 1 cut(s) 101
Psp6I CCWGG 2 cut(s) 350, 706
PspFI CCCAGC 1 cut(s) 422
PspGI CCWGG 2 cut(s) 350, 706
PspN4I GGNNCC 2 cut(s) 94, 292
RsaI GTAC 1 cut(s) 292
RsaNI GTAC 1 cut(s) 291
RseI CAYNNNNRTG 2 cut(s) 271, 392
SaqAI TTAA 4 cut(s) 213, 489, 564, 630
SatI GCNGC 2 cut(s) 96, 192
Sau3AI GATC 5 cut(s) 157, 246, 262, 334, 621
ScrFI CCNGG 2 cut(s) 352, 708
SduI GDGCHC 1 cut(s) 39
SetI ASST 8 cut(s) 104, 196, 292, 424, 482, 489, 693, 734
SfaNI GCATC 1 cut(s) 67
Sfr274I CTCGAG 1 cut(s) 182
SlaI CTCGAG 1 cut(s) 182
SmiMI CAYNNNNRTG 2 cut(s) 271, 392
SmlI CTYRAG 1 cut(s) 182
SmoI CTYRAG 1 cut(s) 182
SpeI ACTAGT 1 cut(s) 668
SphI GCATGC 1 cut(s) 65
Sse9I AATT 7 cut(s) 328, 405, 429, 574, 712, 719, 748
SsiI CCGC 2 cut(s) 96, 120
SspI AATATT 1 cut(s) 700
SspMI CTAG 1 cut(s) 669
StyD4I CCNGG 2 cut(s) 350, 706
StyI CCWWGG 1 cut(s) 66
TaaI ACNGT 1 cut(s) 210
TaiI ACGT 1 cut(s) 104
TaqI TCGA 1 cut(s) 183
TasI AATT 7 cut(s) 328, 405, 429, 574, 712, 719, 748
TauI GCSGC 1 cut(s) 98
Tru1I TTAA 4 cut(s) 213, 489, 564, 630
Tru9I TTAA 4 cut(s) 213, 489, 564, 630
TscAI CASTG 3 cut(s) 39, 213, 310
TseFI GTSAC 2 cut(s) 110, 661
TseI GCWGC 1 cut(s) 191
Tsp45I GTSAC 2 cut(s) 110, 661
TspDTI ATGAA 1 cut(s) 750
TspRI CASTG 3 cut(s) 39, 213, 310
XceI RCATGY 1 cut(s) 65
XcmI CCANNNNNNNNNTGG 1 cut(s) 173
XhoI CTCGAG 1 cut(s) 182
XspI CTAG 1 cut(s) 669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.