Rorug05G0341900

expansin-A23-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001146
Physical Location & Seq
Reverse (-)
41808632 .. 41810147
1516 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0341900.1

Sequence Viewer

Length: 1221 bp
ATGGAGGCCGAATTTGATATTCCGTCCCACTTTCTCTGCCCCATCTCTCTTGAAGTCATGAGAGATCCCGTCACGGTCTCTACTGGGATTACATACGATCGGGAAAGCATCGAGAGATGGTTATCGTCCGGCAAGAACAAGGCTTGCCCTGTCACCAAACAACAATTGTTCGACGCAGATCTCACTCCCAACCACAATCTTCGTCGCATAATCCAAGCTTGGTGCACCCTCAACGCATCTCATGGGGTCGAGCGGATACCAACGCCAACATCGCAGCTTGACAAGACCCAGATCGTTAAGCTGATCGACCAAGCGAGGAAGCTCCCCCACACGCAGCTCCAATGCCTCAAAAGGCTCAGATCCCTGGCCGTAAACCACGGTGAAAGAAACAAGAACTCTTTGGAGGCAGCAGGAGCAGTAGAATACGTTGCTTCTTTGATAAGCAACGCCGACCAATCCATAATGAAAGTGGCTGTCGAAGTCTTGTGCCATCTCAGAAGCTCGGAATCTCGTCTCAAAGATCTAATGAGCAAAGATGGGGAGAAGATCATAGATTCATTGGTGCAAGTATTAAAACGAGGTGATTACCAATCTCGAGCTCATGCAACTATTTTATTGAGGTCTGTTTTCGAATTGGCAAATCCGATGCGGTTGATGAGTGCCACAGTTGAGTTATTCATCGAGACTATGAATGTGGTACGAGATCAGATCTCACAGCAAGCATTTAAAGCTGCATTGAAGCTTCTTTTGGAGCTATGTCCATGGGGAAGGAACCGAATAAAGGCCGTGGAAGGAGGCGCAGTTTCGATCCTCATAGAGGCTCTTCTCGAGACTCAAGAGAAGAGGGCATGTGAGCTGATACTAATCTCTTTGGACCAGCTCTGTCGATGCGCGGAAGGGCGAGCCGAGTTATTGAGGCACGCGGCGGGGCTAGCCATCGTGTCCAAGAAGATATTTAGGGTTTCGCACGTGGCGAGCGACAGGGCGGTGAGGATATTGTTTTCCATTTGTAGATTCTCTGCAACTTCTGCAGTTGTTATGGAAATGTTGCAAGTAGGTGTGGTGGCCAAGTTGTGTTTAGTGCTTCAAGTGGATTGTAGGTTTAAGACCAAAGAGAAGGCTAGGGAGATTCTCGAATTGCACTCTAGGGTTTGGAAGAGTTCTCCATGCGTTCCTGCTCATTTGTTGTCCTTTTATCCACCAACATCGTCTTCCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

406

Amino Acids

45.4

Weight (kDa)

9.07

Isoelectric Point (pI)

47.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
U-box PF04564 7 - 76 2.7e-20 U-box domain
ARM_PUB PF25598 97 - 396 6.3e-79 Plant U-box protein ARM repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 253
AccII CGCG 2 cut(s) 893, 923
AciI CCGC 6 cut(s) 253, 649, 893, 923, 926, 986
AclWI GGATC 3 cut(s) 59, 354, 802
AcoI YGGCCR 2 cut(s) 366, 1065
AcsI RAATTY 1 cut(s) 11
AcvI CACGTG 1 cut(s) 970
AfaI GTAC 1 cut(s) 699
AfiI CCNNNNNNNGG 2 cut(s) 781, 817
AgsI TTSAA 3 cut(s) 53, 739, 1088
AjnI CCWGG 1 cut(s) 363
AjuI GAANNNNNNNTTGG 3 cut(s) 731, 763, 1195
Alw21I GWGCWC 2 cut(s) 227, 601
Alw26I GTCTC 4 cut(s) 82, 518, 677, 824
Alw44I GTGCAC 1 cut(s) 223
AlwI GGATC 3 cut(s) 59, 354, 802
Ama87I CYCGRG 2 cut(s) 594, 827
AoxI GGCC 4 cut(s) 6, 366, 783, 1065
ApaLI GTGCAC 1 cut(s) 223
ApeKI GCWGC 4 cut(s) 274, 334, 407, 731
ApoI RAATTY 1 cut(s) 11
AspLEI GCGC 2 cut(s) 800, 893
AspS9I GGNCC 1 cut(s) 874
AsuHPI GGTGA 4 cut(s) 145, 392, 593, 1000
AsuII TTCGAA 1 cut(s) 630
AsuNHI GCTAGC 1 cut(s) 931
AvaI CYCGRG 2 cut(s) 594, 827
AvaII GGWCC 1 cut(s) 874
BaeGI GKGCMC 1 cut(s) 227
BalI TGGCCA 1 cut(s) 1067
BanII GRGCYC 1 cut(s) 601
BbrPI CACGTG 1 cut(s) 970
BbsI GAAGAC 1 cut(s) 1203
Bbv12I GWGCWC 2 cut(s) 227, 601
BbvI GCAGC 4 cut(s) 286, 346, 419, 718
BccI CCATC 5 cut(s) 50, 111, 498, 530, 944
BceAI ACGGC 2 cut(s) 353, 770
BciT130I CCWGG 1 cut(s) 365
BciVI GTATCC 1 cut(s) 249
BcoDI GTCTC 4 cut(s) 82, 518, 677, 824
BfaI CTAG 3 cut(s) 932, 1122, 1146
BfmI CTRYAG 1 cut(s) 1029
BfuI GTATCC 1 cut(s) 249
BglII AGATCT 3 cut(s) 178, 520, 708
BisI GCNGC 5 cut(s) 275, 335, 408, 732, 924
BlsI GCNGC 5 cut(s) 276, 336, 409, 733, 925
Bme1390I CCNGG 1 cut(s) 365
Bme18I GGWCC 1 cut(s) 874
BmeT110I CYCGRG 2 cut(s) 594, 827
BmgT120I GGNCC 1 cut(s) 874
BmiI GGNNCC 1 cut(s) 773
BmrFI CCNGG 1 cut(s) 365
BmrI ACTGGG 1 cut(s) 93
BmsI GCATC 4 cut(s) 117, 245, 636, 878
BmtI GCTAGC 1 cut(s) 935
BmuI ACTGGG 1 cut(s) 93
BpiI GAAGAC 1 cut(s) 1203
Bpu14I TTCGAA 1 cut(s) 630
BpuEI CTTGAG 1 cut(s) 819
BsaAI YACGTR 1 cut(s) 970
BsaBI GATNNNNATC 2 cut(s) 121, 863
BsaI GGTCTC 1 cut(s) 82
BsaJI CCNNGG 4 cut(s) 363, 376, 761, 786
BsaXI ACNNNNNCTCC 2 cut(s) 786, 816
Bsc4I CCNNNNNNNGG 2 cut(s) 781, 817
Bse1I ACTGG 1 cut(s) 88
Bse8I GATNNNNATC 2 cut(s) 121, 863
BseBI CCWGG 1 cut(s) 365
BseDI CCNNGG 4 cut(s) 363, 376, 761, 786
BseJI GATNNNNATC 2 cut(s) 121, 863
BseLI CCNNNNNNNGG 2 cut(s) 781, 817
BseMII CTCAG 2 cut(s) 370, 508
BseNI ACTGG 1 cut(s) 88
BseSI GKGCMC 1 cut(s) 227
BseXI GCAGC 4 cut(s) 286, 346, 419, 718
Bsh1236I CGCG 2 cut(s) 893, 923
Bsh1285I CGRYCG 1 cut(s) 100
BshFI GGCC 4 cut(s) 8, 368, 785, 1067
BsiEI CGRYCG 1 cut(s) 100
BsiHKAI GWGCWC 2 cut(s) 227, 601
BsiHKCI CYCGRG 2 cut(s) 594, 827
BsiSI CCGG 1 cut(s) 129
BslFI GGGAC 1 cut(s) 10
BslI CCNNNNNNNGG 2 cut(s) 781, 817
BsmAI GTCTC 4 cut(s) 82, 518, 677, 824
BsmBI CGTCTC 1 cut(s) 518
BsmFI GGGAC 1 cut(s) 10
BsnI GGCC 4 cut(s) 8, 368, 785, 1067
Bso31I GGTCTC 1 cut(s) 82
BsoBI CYCGRG 2 cut(s) 594, 827
Bsp119I TTCGAA 1 cut(s) 630
Bsp1286I GDGCHC 2 cut(s) 227, 601
Bsp19I CCATGG 1 cut(s) 761
BspACI CCGC 6 cut(s) 253, 649, 893, 923, 926, 986
BspANI GGCC 4 cut(s) 8, 368, 785, 1067
BspCNI CTCAG 2 cut(s) 369, 507
BspFNI CGCG 2 cut(s) 893, 923
BspHI TCATGA 1 cut(s) 57
BspLI GGNNCC 1 cut(s) 773
BspMAI CTGCAG 1 cut(s) 1033
BspOI GCTAGC 1 cut(s) 935
BspPI GGATC 3 cut(s) 59, 354, 802
BspQI GCTCTTC 1 cut(s) 828
BspT104I TTCGAA 1 cut(s) 630
BspTNI GGTCTC 1 cut(s) 82
BsrBI CCGCTC 1 cut(s) 253
BsrI ACTGG 1 cut(s) 88
BssECI CCNNGG 4 cut(s) 363, 376, 761, 786
BssT1I CCWWGG 1 cut(s) 761
Bst2UI CCWGG 1 cut(s) 365
Bst4CI ACNGT 3 cut(s) 76, 380, 667
Bst6I CTCTTC 3 cut(s) 828, 836, 1151
BstAPI GCANNNNNTGC 1 cut(s) 1028
BstBAI YACGTR 1 cut(s) 970
BstBI TTCGAA 1 cut(s) 630
BstC8I GCNNGC 6 cut(s) 145, 720, 903, 921, 933, 976
BstDEI CTNAG 2 cut(s) 356, 494
BstDSI CCRYGG 3 cut(s) 376, 761, 786
BstENI CCTNNNNNAGG 1 cut(s) 815
BstFNI CGCG 2 cut(s) 893, 923
BstHHI GCGC 2 cut(s) 800, 893
BstMAI GTCTC 4 cut(s) 82, 518, 677, 824
BstMCI CGRYCG 1 cut(s) 100
BstMWI GCNNNNNNNGC 5 cut(s) 271, 413, 728, 932, 1028
BstNI CCWGG 1 cut(s) 365
BstNSI RCATGY 1 cut(s) 852
BstSCI CCNGG 1 cut(s) 363
BstSFI CTRYAG 1 cut(s) 1029
BstSLI GKGCMC 1 cut(s) 227
BstUI CGCG 2 cut(s) 893, 923
BstV1I GCAGC 4 cut(s) 286, 346, 419, 718
BstV2I GAAGAC 1 cut(s) 1203
BstX2I RGATCY 5 cut(s) 64, 178, 359, 520, 708
BstYI RGATCY 5 cut(s) 64, 178, 359, 520, 708
BsuI GTATCC 1 cut(s) 249
BsuRI GGCC 4 cut(s) 8, 368, 785, 1067
BtgI CCRYGG 3 cut(s) 376, 761, 786
BtgZI GCGATG 1 cut(s) 255
Cac8I GCNNGC 6 cut(s) 145, 720, 903, 921, 933, 976
CciI TCATGA 1 cut(s) 57
CfoI GCGC 2 cut(s) 800, 893
Cfr13I GGNCC 1 cut(s) 874
CseI GACGC 1 cut(s) 182
Csp6I GTAC 1 cut(s) 698
CviAII CATG 6 cut(s) 58, 242, 602, 762, 849, 1167
CviQI GTAC 1 cut(s) 698
DdeI CTNAG 2 cut(s) 356, 494
DraI TTTAAA 1 cut(s) 727
EaeI YGGCCR 2 cut(s) 366, 1065
Eam1104I CTCTTC 3 cut(s) 828, 836, 1151
EarI CTCTTC 3 cut(s) 828, 836, 1151
Ecl136II GAGCTC 1 cut(s) 599
Eco130I CCWWGG 1 cut(s) 761
Eco24I GRGCYC 1 cut(s) 601
Eco31I GGTCTC 1 cut(s) 82
Eco47I GGWCC 1 cut(s) 874
Eco53kI GAGCTC 1 cut(s) 599
Eco72I CACGTG 1 cut(s) 970
Eco88I CYCGRG 2 cut(s) 594, 827
EcoICRI GAGCTC 1 cut(s) 599
EcoNI CCTNNNNNAGG 1 cut(s) 815
EcoRII CCWGG 1 cut(s) 363
EcoT14I CCWWGG 1 cut(s) 761
EcoT38I GRGCYC 1 cut(s) 601
ErhI CCWWGG 1 cut(s) 761
Esp3I CGTCTC 1 cut(s) 518
FaeI CATG 6 cut(s) 61, 245, 605, 765, 852, 1170
FaqI GGGAC 1 cut(s) 10
FatI CATG 6 cut(s) 57, 241, 601, 761, 848, 1166
FauI CCCGC 1 cut(s) 919
Fnu4HI GCNGC 5 cut(s) 275, 335, 408, 732, 924
FriOI GRGCYC 1 cut(s) 601
Fsp4HI GCNGC 5 cut(s) 275, 335, 408, 732, 924
FspBI CTAG 3 cut(s) 932, 1122, 1146
GlaI GCGC 2 cut(s) 799, 892
GluI GCNGC 5 cut(s) 275, 335, 408, 732, 924
HaeIII GGCC 4 cut(s) 8, 368, 785, 1067
HapII CCGG 1 cut(s) 129
HgaI GACGC 1 cut(s) 182
HhaI GCGC 2 cut(s) 800, 893
Hin1II CATG 6 cut(s) 61, 245, 605, 765, 852, 1170
Hin6I GCGC 2 cut(s) 798, 891
HinP1I GCGC 2 cut(s) 798, 891
HindIII AAGCTT 2 cut(s) 216, 740
HinfI GANTC 5 cut(s) 506, 554, 832, 1014, 1129
HpaII CCGG 1 cut(s) 129
HphI GGTGA 4 cut(s) 145, 392, 593, 1000
Hpy166II GTNNAC 2 cut(s) 225, 373
Hpy188I TCNGA 5 cut(s) 359, 497, 505, 645, 708
Hpy8I GTNNAC 2 cut(s) 225, 373
Hpy99I CGWCG 2 cut(s) 176, 207
HpyAV CCTTC 4 cut(s) 762, 785, 890, 1111
HpyCH4III ACNGT 3 cut(s) 76, 380, 667
HpyCH4IV ACGT 2 cut(s) 426, 969
HpyCH4V TGCA 8 cut(s) 225, 565, 605, 734, 1022, 1031, 1051, 1141
HpyF10VI GCNNNNNNNGC 5 cut(s) 271, 413, 728, 932, 1028
HpyF3I CTNAG 2 cut(s) 356, 494
HpySE526I ACGT 2 cut(s) 426, 969
Hsp92II CATG 6 cut(s) 61, 245, 605, 765, 852, 1170
HspAI GCGC 2 cut(s) 798, 891
LguI GCTCTTC 1 cut(s) 828
LmnI GCTCC 4 cut(s) 327, 342, 413, 751
Lsp1109I GCAGC 4 cut(s) 286, 346, 419, 718
LweI GCATC 4 cut(s) 117, 245, 636, 878
MaeI CTAG 3 cut(s) 932, 1122, 1146
MaeII ACGT 2 cut(s) 426, 969
MaeIII GTNAC 2 cut(s) 70, 151
MbiI CCGCTC 1 cut(s) 253
MboII GAAGA 7 cut(s) 191, 556, 815, 853, 961, 1168, 1203
MfeI CAATTG 1 cut(s) 164
MflI RGATCY 5 cut(s) 64, 178, 359, 520, 708
MhlI GDGCHC 2 cut(s) 227, 601
MlsI TGGCCA 1 cut(s) 1067
MluCI AATT 4 cut(s) 11, 164, 632, 1136
MluNI TGGCCA 1 cut(s) 1067
MlyI GAGTC 1 cut(s) 826
Mox20I TGGCCA 1 cut(s) 1067
MscI TGGCCA 1 cut(s) 1067
MseI TTAA 4 cut(s) 297, 572, 726, 1104
Msp20I TGGCCA 1 cut(s) 1067
MspI CCGG 1 cut(s) 129
MspR9I CCNGG 1 cut(s) 365
MunI CAATTG 1 cut(s) 164
MvaI CCWGG 1 cut(s) 365
MvnI CGCG 2 cut(s) 893, 923
MwoI GCNNNNNNNGC 5 cut(s) 271, 413, 728, 932, 1028
NcoI CCATGG 1 cut(s) 761
NheI GCTAGC 1 cut(s) 931
NlaIII CATG 6 cut(s) 61, 245, 605, 765, 852, 1170
NlaIV GGNNCC 1 cut(s) 773
NmeAIII GCCGAG 1 cut(s) 931
NmuCI GTSAC 2 cut(s) 70, 151
NspI RCATGY 1 cut(s) 852
NspV TTCGAA 1 cut(s) 630
PaeR7I CTCGAG 2 cut(s) 594, 827
PagI TCATGA 1 cut(s) 57
PciSI GCTCTTC 1 cut(s) 828
PcsI WCGNNNNNNNCGW 2 cut(s) 971, 975
PfeI GAWTC 4 cut(s) 506, 554, 1014, 1129
PkrI GCNGC 5 cut(s) 276, 336, 409, 733, 925
Ple19I CGATCG 1 cut(s) 100
PleI GAGTC 1 cut(s) 826
PmaCI CACGTG 1 cut(s) 970
PmlI CACGTG 1 cut(s) 970
PpsI GAGTC 1 cut(s) 826
Ppu21I YACGTR 1 cut(s) 970
Psp124BI GAGCTC 1 cut(s) 601
Psp6I CCWGG 1 cut(s) 363
PspCI CACGTG 1 cut(s) 970
PspGI CCWGG 1 cut(s) 363
PspN4I GGNNCC 1 cut(s) 773
PspPI GGNCC 1 cut(s) 874
PstI CTGCAG 1 cut(s) 1033
PsuI RGATCY 5 cut(s) 64, 178, 359, 520, 708
PvuI CGATCG 1 cut(s) 100
RsaI GTAC 1 cut(s) 699
RsaNI GTAC 1 cut(s) 698
SacI GAGCTC 1 cut(s) 601
SapI GCTCTTC 1 cut(s) 828
SaqAI TTAA 4 cut(s) 297, 572, 726, 1104
SatI GCNGC 5 cut(s) 275, 335, 408, 732, 924
Sau96I GGNCC 1 cut(s) 874
SchI GAGTC 1 cut(s) 826
ScrFI CCNGG 1 cut(s) 365
SduI GDGCHC 2 cut(s) 227, 601
SfaNI GCATC 4 cut(s) 117, 245, 636, 878
SfcI CTRYAG 1 cut(s) 1029
Sfr274I CTCGAG 2 cut(s) 594, 827
SfuI TTCGAA 1 cut(s) 630
SinI GGWCC 1 cut(s) 874
SlaI CTCGAG 2 cut(s) 594, 827
SmlI CTYRAG 3 cut(s) 594, 827, 834
SmoI CTYRAG 3 cut(s) 594, 827, 834
Sse9I AATT 4 cut(s) 11, 164, 632, 1136
SsiI CCGC 6 cut(s) 253, 649, 893, 923, 926, 986
SspMI CTAG 3 cut(s) 932, 1122, 1146
SstI GAGCTC 1 cut(s) 601
StyD4I CCNGG 1 cut(s) 363
StyI CCWWGG 1 cut(s) 761
TaaI ACNGT 3 cut(s) 76, 380, 667
TaiI ACGT 2 cut(s) 429, 972
TasI AATT 4 cut(s) 11, 164, 632, 1136
TauI GCSGC 1 cut(s) 926
TfiI GAWTC 4 cut(s) 506, 554, 1014, 1129
Tru1I TTAA 4 cut(s) 297, 572, 726, 1104
Tru9I TTAA 4 cut(s) 297, 572, 726, 1104
TseFI GTSAC 2 cut(s) 70, 151
TseI GCWGC 4 cut(s) 274, 334, 407, 731
Tsp45I GTSAC 2 cut(s) 70, 151
TspDTI ATGAA 4 cut(s) 479, 546, 667, 704
TspGWI ACGGA 1 cut(s) 12
VneI GTGCAC 1 cut(s) 223
VpaK11BI GGWCC 1 cut(s) 874
XagI CCTNNNNNAGG 1 cut(s) 815
XapI RAATTY 1 cut(s) 11
XceI RCATGY 1 cut(s) 852
XcmI CCANNNNNNNNNTGG 1 cut(s) 466
XhoI CTCGAG 2 cut(s) 594, 827
XspI CTAG 3 cut(s) 932, 1122, 1146
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.