RLG00000015302

expansin-A23-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0001146
Physical Location & Seq
Reverse (-)
64224047 .. 64225089
1043 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015302

Sequence Viewer

Length: 771 bp
ATGAGTAAGTTCCAGAACTTGTTCGGAGCGGCGACAGTGTTCATCATCATTCTTTCGGTCCAAACCATGAGCAATCCAATCGGCGAGAGTCCTGACCATGACTGGCACGATGGACATGCAACCTTCTACGGCGACATGAATGGAAGCGAAACCATGCAGGGGGCTTGCGGATATGGTGATCTCTTCCAACAAGGTTATGGTCTAGATACAGCAGCTCTAAGCACAACATTGTTCAACAATGGATTTACTTGCGGTGCTTGTTTCGAGATCATGTGCGTCCATGATCCACAATGGTGCATCCCTAATGCAGGCACGATCAAAATTACTGCAACAAATTTTTGTCCACCAAACTGGGCTCAAGGAGGATGGTGCAATCCGCCATACGATCACTTCGACTTGTCCATGCCTATGTTCACAAAAATCGCGCACTACAAAGCTGGAATAGTTCCGGTCAAGTTTCGCCGAGTCCCGTGTGCTAAAACAGGAGGGGTTAAGTTTGAGCTTAAAGGAAATACCTACTGGATTACTGCTTTGGTTTACAATGTGGGTGGTGCCGGTGATGTTAACGCTGTCAGAATCAAAGGATCTAATTCCGGTTGGCTTCAAATGTCGCGCAATTGGGGCCAAGTTTGGCAGACCAGCGCAGAGTTAGTAGGGCAAAGCTTGTCATTCCAAGTCACTACCAGTGAAGACAAAACTATTGAGTTTGATAATGTTGCACCAGGGAATTGGCAATTTGGTCAAACATATGAGGGGAATGGTAACTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

257

Amino Acids

28.08

Weight (kDa)

5.41

Isoelectric Point (pI)

29.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EXPB1_D1 PF22514 41 - 158 3.7e-06 EXPB1-like domain 1
DPBB_1 PF03330 70 - 153 4.2e-20 Lytic transglycolase
Expansin_C PF01357 164 - 241 7.3e-28 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 551
AccBSI CCGCTC 1 cut(s) 29
AccII CGCG 2 cut(s) 425, 613
AciI CCGC 4 cut(s) 29, 168, 252, 377
AclWI GGATC 2 cut(s) 278, 592
AcsI RAATTY 1 cut(s) 334
AfiI CCNNNNNNNGG 2 cut(s) 159, 308
AgsI TTSAA 2 cut(s) 235, 605
AjnI CCWGG 1 cut(s) 721
AleI CACNNNNGTG 1 cut(s) 292
AluBI AGCT 4 cut(s) 215, 437, 502, 663
AluI AGCT 4 cut(s) 215, 437, 502, 663
AlwI GGATC 2 cut(s) 278, 592
AoxI GGCC 1 cut(s) 622
ApeKI GCWGC 1 cut(s) 212
ApoI RAATTY 1 cut(s) 334
Asp700I GAANNNNTTC 1 cut(s) 20
AspLEI GCGC 3 cut(s) 427, 615, 644
AspS9I GGNCC 2 cut(s) 58, 622
AsuHPI GGTGA 2 cut(s) 188, 569
AvaII GGWCC 1 cut(s) 58
BanI GGYRCC 1 cut(s) 551
BanII GRGCYC 1 cut(s) 358
BbsI GAAGAC 1 cut(s) 696
BbvI GCAGC 1 cut(s) 224
BccI CCATC 2 cut(s) 104, 360
BceAI ACGGC 1 cut(s) 145
BcgI CGANNNNNNTGC 4 cut(s) 61, 95, 98, 132
BciT130I CCWGG 1 cut(s) 723
BfaI CTAG 1 cut(s) 203
BisI GCNGC 2 cut(s) 30, 213
BlsI GCNGC 2 cut(s) 31, 214
Bme1390I CCNGG 1 cut(s) 723
Bme18I GGWCC 1 cut(s) 58
BmgT120I GGNCC 2 cut(s) 58, 622
BmiI GGNNCC 2 cut(s) 553, 623
BmrFI CCNGG 1 cut(s) 723
BmrI ACTGGG 1 cut(s) 361
BmsI GCATC 1 cut(s) 306
BmuI ACTGGG 1 cut(s) 361
BpiI GAAGAC 1 cut(s) 696
BpuEI CTTGAG 1 cut(s) 342
BsaJI CCNNGG 1 cut(s) 722
BsaWI WCCGGW 2 cut(s) 448, 593
Bsc4I CCNNNNNNNGG 2 cut(s) 159, 308
Bse118I RCCGGY 1 cut(s) 554
Bse1I ACTGG 4 cut(s) 107, 356, 524, 684
BseBI CCWGG 1 cut(s) 723
BseDI CCNNGG 1 cut(s) 722
BseGI GGATG 2 cut(s) 297, 371
BseLI CCNNNNNNNGG 2 cut(s) 159, 308
BseNI ACTGG 4 cut(s) 107, 356, 524, 684
BseXI GCAGC 1 cut(s) 224
Bsh1236I CGCG 2 cut(s) 425, 613
BshFI GGCC 1 cut(s) 624
BshNI GGYRCC 1 cut(s) 551
BsiSI CCGG 3 cut(s) 449, 555, 594
BslFI GGGAC 1 cut(s) 452
BslI CCNNNNNNNGG 2 cut(s) 159, 308
BsmFI GGGAC 1 cut(s) 452
BsnI GGCC 1 cut(s) 624
Bsp1286I GDGCHC 1 cut(s) 358
Bsp143I GATC 6 cut(s) 178, 267, 283, 315, 385, 584
BspACI CCGC 4 cut(s) 29, 168, 252, 377
BspANI GGCC 1 cut(s) 624
BspFNI CGCG 2 cut(s) 425, 613
BspLI GGNNCC 2 cut(s) 553, 623
BspPI GGATC 2 cut(s) 278, 592
BspT107I GGYRCC 1 cut(s) 551
BsrBI CCGCTC 1 cut(s) 29
BsrFI RCCGGY 1 cut(s) 554
BsrI ACTGG 4 cut(s) 107, 356, 524, 684
BssAI RCCGGY 1 cut(s) 554
BssECI CCNNGG 1 cut(s) 722
BssMI GATC 6 cut(s) 178, 267, 283, 315, 385, 584
Bst2UI CCWGG 1 cut(s) 723
Bst4CI ACNGT 1 cut(s) 37
Bst6I CTCTTC 1 cut(s) 188
BstC8I GCNNGC 2 cut(s) 166, 310
BstDEI CTNAG 1 cut(s) 218
BstENI CCTNNNNNAGG 1 cut(s) 306
BstF5I GGATG 2 cut(s) 297, 371
BstFNI CGCG 2 cut(s) 425, 613
BstHHI GCGC 3 cut(s) 427, 615, 644
BstKTI GATC 6 cut(s) 181, 270, 286, 318, 388, 587
BstMBI GATC 6 cut(s) 178, 267, 283, 315, 385, 584
BstMWI GCNNNNNNNGC 1 cut(s) 621
BstNI CCWGG 1 cut(s) 723
BstNSI RCATGY 1 cut(s) 119
BstSCI CCNGG 1 cut(s) 721
BstUI CGCG 2 cut(s) 425, 613
BstV1I GCAGC 1 cut(s) 224
BstV2I GAAGAC 1 cut(s) 696
BstX2I RGATCY 1 cut(s) 584
BstXI CCANNNNNNTGG 2 cut(s) 351, 729
BstYI RGATCY 1 cut(s) 584
BsuRI GGCC 1 cut(s) 624
BtsCI GGATG 2 cut(s) 297, 371
BtsIMutI CAGTG 2 cut(s) 42, 691
Cac8I GCNNGC 2 cut(s) 166, 310
CfoI GCGC 3 cut(s) 427, 615, 644
Cfr10I RCCGGY 1 cut(s) 554
Cfr13I GGNCC 2 cut(s) 58, 622
CseI GACGC 1 cut(s) 265
CspCI CAANNNNNGTGG 2 cut(s) 529, 564
CviAII CATG 8 cut(s) 67, 98, 116, 136, 154, 271, 281, 403
CviJI RGCY 8 cut(s) 164, 215, 356, 437, 502, 601, 624, 663
CviKI_1 RGCY 8 cut(s) 164, 215, 356, 437, 502, 601, 624, 663
DdeI CTNAG 1 cut(s) 218
DpnI GATC 6 cut(s) 180, 269, 285, 317, 387, 586
DpnII GATC 6 cut(s) 178, 267, 283, 315, 385, 584
Eam1104I CTCTTC 1 cut(s) 188
EarI CTCTTC 1 cut(s) 188
EciI GGCGGA 1 cut(s) 366
Eco24I GRGCYC 1 cut(s) 358
Eco47I GGWCC 1 cut(s) 58
EcoNI CCTNNNNNAGG 1 cut(s) 306
EcoRII CCWGG 1 cut(s) 721
EcoT38I GRGCYC 1 cut(s) 358
FaeI CATG 8 cut(s) 70, 101, 119, 139, 157, 274, 284, 406
FaqI GGGAC 1 cut(s) 452
FatI CATG 8 cut(s) 66, 97, 115, 135, 153, 270, 280, 402
FauNDI CATATG 1 cut(s) 748
Fnu4HI GCNGC 2 cut(s) 30, 213
FokI GGATG 2 cut(s) 284, 378
FriOI GRGCYC 1 cut(s) 358
Fsp4HI GCNGC 2 cut(s) 30, 213
FspBI CTAG 1 cut(s) 203
GlaI GCGC 3 cut(s) 426, 614, 643
GluI GCNGC 2 cut(s) 30, 213
HaeIII GGCC 1 cut(s) 624
HapII CCGG 3 cut(s) 449, 555, 594
HgaI GACGC 1 cut(s) 265
HhaI GCGC 3 cut(s) 427, 615, 644
Hin1II CATG 8 cut(s) 70, 101, 119, 139, 157, 274, 284, 406
Hin6I GCGC 3 cut(s) 425, 613, 642
HinP1I GCGC 3 cut(s) 425, 613, 642
HincII GTYRAC 1 cut(s) 565
HindII GTYRAC 1 cut(s) 565
HindIII AAGCTT 1 cut(s) 661
HinfI GANTC 3 cut(s) 88, 465, 576
HpaI GTTAAC 1 cut(s) 565
HpaII CCGG 3 cut(s) 449, 555, 594
HphI GGTGA 2 cut(s) 188, 569
Hpy166II GTNNAC 4 cut(s) 344, 414, 538, 565
Hpy188I TCNGA 2 cut(s) 26, 575
Hpy188III TCNNGA 4 cut(s) 13, 92, 203, 265
Hpy8I GTNNAC 4 cut(s) 344, 414, 538, 565
HpyAV CCTTC 1 cut(s) 133
HpyCH4III ACNGT 1 cut(s) 37
HpyCH4V TGCA 7 cut(s) 119, 157, 297, 308, 329, 372, 719
HpyF10VI GCNNNNNNNGC 1 cut(s) 621
HpyF3I CTNAG 1 cut(s) 218
Hsp92II CATG 8 cut(s) 70, 101, 119, 139, 157, 274, 284, 406
HspAI GCGC 3 cut(s) 425, 613, 642
KspAI GTTAAC 1 cut(s) 565
Kzo9I GATC 6 cut(s) 178, 267, 283, 315, 385, 584
LmnI GCTCC 1 cut(s) 26
Lsp1109I GCAGC 1 cut(s) 224
LweI GCATC 1 cut(s) 306
MaeI CTAG 1 cut(s) 203
MaeIII GTNAC 2 cut(s) 676, 761
MalI GATC 6 cut(s) 180, 269, 285, 317, 387, 586
MbiI CCGCTC 1 cut(s) 29
MboI GATC 6 cut(s) 178, 267, 283, 315, 385, 584
MboII GAAGA 2 cut(s) 175, 701
MfeI CAATTG 1 cut(s) 616
MflI RGATCY 1 cut(s) 584
MhlI GDGCHC 1 cut(s) 358
MluCI AATT 6 cut(s) 321, 334, 589, 616, 727, 734
MlyI GAGTC 2 cut(s) 97, 474
MmeI TCCRAC 1 cut(s) 211
MnlI CCTC 3 cut(s) 356, 479, 745
MroXI GAANNNNTTC 1 cut(s) 20
MseI TTAA 3 cut(s) 492, 504, 564
MslI CAYNNNNRTG 2 cut(s) 292, 407
MspI CCGG 3 cut(s) 449, 555, 594
MspR9I CCNGG 1 cut(s) 723
MunI CAATTG 1 cut(s) 616
MvaI CCWGG 1 cut(s) 723
MvnI CGCG 2 cut(s) 425, 613
MwoI GCNNNNNNNGC 1 cut(s) 621
NdeI CATATG 1 cut(s) 748
NdeII GATC 6 cut(s) 178, 267, 283, 315, 385, 584
NlaIII CATG 8 cut(s) 70, 101, 119, 139, 157, 274, 284, 406
NlaIV GGNNCC 2 cut(s) 553, 623
NmeAIII GCCGAG 1 cut(s) 488
NmuCI GTSAC 1 cut(s) 676
NspI RCATGY 1 cut(s) 119
OliI CACNNNNGTG 1 cut(s) 292
PcsI WCGNNNNNNNCGW 1 cut(s) 390
PdmI GAANNNNTTC 1 cut(s) 20
PfeI GAWTC 1 cut(s) 576
PkrI GCNGC 2 cut(s) 31, 214
PleI GAGTC 2 cut(s) 96, 473
PpsI GAGTC 2 cut(s) 96, 473
Psp6I CCWGG 1 cut(s) 721
PspGI CCWGG 1 cut(s) 721
PspN4I GGNNCC 2 cut(s) 553, 623
PspPI GGNCC 2 cut(s) 58, 622
PsuI RGATCY 1 cut(s) 584
RseI CAYNNNNRTG 2 cut(s) 292, 407
SaqAI TTAA 3 cut(s) 492, 504, 564
SatI GCNGC 2 cut(s) 30, 213
Sau3AI GATC 6 cut(s) 178, 267, 283, 315, 385, 584
Sau96I GGNCC 2 cut(s) 58, 622
SchI GAGTC 2 cut(s) 97, 474
ScrFI CCNGG 1 cut(s) 723
SduI GDGCHC 1 cut(s) 358
SetI ASST 7 cut(s) 125, 196, 217, 439, 504, 518, 665
SfaNI GCATC 1 cut(s) 306
SinI GGWCC 1 cut(s) 58
SmiMI CAYNNNNRTG 2 cut(s) 292, 407
SmlI CTYRAG 1 cut(s) 357
SmoI CTYRAG 1 cut(s) 357
Sse9I AATT 6 cut(s) 321, 334, 589, 616, 727, 734
SsiI CCGC 4 cut(s) 29, 168, 252, 377
SspMI CTAG 1 cut(s) 203
StyD4I CCNGG 1 cut(s) 721
TaaI ACNGT 1 cut(s) 37
TaqI TCGA 2 cut(s) 264, 393
TaqII GACCGA 1 cut(s) 46
TasI AATT 6 cut(s) 321, 334, 589, 616, 727, 734
TauI GCSGC 1 cut(s) 32
TfiI GAWTC 1 cut(s) 576
Tru1I TTAA 3 cut(s) 492, 504, 564
Tru9I TTAA 3 cut(s) 492, 504, 564
TscAI CASTG 2 cut(s) 42, 691
TseFI GTSAC 1 cut(s) 676
TseI GCWGC 1 cut(s) 212
Tsp45I GTSAC 1 cut(s) 676
TspDTI ATGAA 2 cut(s) 31, 152
TspRI CASTG 2 cut(s) 42, 691
VpaK11BI GGWCC 1 cut(s) 58
XagI CCTNNNNNAGG 1 cut(s) 306
XapI RAATTY 1 cut(s) 334
XbaI TCTAGA 1 cut(s) 202
XceI RCATGY 1 cut(s) 119
XcmI CCANNNNNNNNNTGG 1 cut(s) 194
XmnI GAANNNNTTC 1 cut(s) 20
XspI CTAG 1 cut(s) 203
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.