AT5G39300

Causes loosening and extension of plant cell walls by disrupting non-covalent bonding between cellulose microfibrils and matrix glucans. No enzymatic activity has been found (By similarity)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001146
Physical Location & Seq
Reverse (-)
15737366 .. 15738479
1114 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G39300.1

Sequence Viewer

Length: 831 bp
ATGAAACTCTTAGAACAAATGGTATACGTAGAATGTTTTATGATAATAATGGCTACATTGTTAGTGTCCATGAGTTACGGTCATCGAGCGATGATTAATGATGTAGCCGAAGCACCCGTGATTGACAATGTAGGCAGCCCAACCAACGGACTCGACAGTTCTTGGTATGATGCACGAGCAACATTTTACGGTGATATCCATGGTGGAGAAACTCAACAGGGAGCCTGTGGATACGGTGATTTATTCAAACAAGGCTATGGTCTAGAGACAGCGGCATTGAGCACAGCACTCTTCAACGAAGGGTATACATGTGGGGCTTGTTACCAGATCATGTGTGTGCACGACCCACAATGGTGTTTACCCGGAACCATCAAGATTACAGCAACAAATTTCTGTCCACCAGATTACTCCAAAACCGAAGGCGTTTGGTGCAACCCACCACAAAAGCACTTTGACCTCTCCCTACCAATGTTCCTCAAGATCGCCCAATACAAAGCTGGAGTTGTCCCAGTTAAATACAGACGTATTTCTTGTGCAAGAACTGGTGGTGTCAAGTTTGAAACCAAAGGAAACCCTTATTTCTTAATGATCTTGCCATACAATGTAGGAGGCGCTGGAGATATCAAGTTGATGCAAGTTAAAGGAGACAAGACCGGGTGGATAACAATGCAAAAGAACTGGGGACAAAACTGGACCACTGGTGTTAATTTGACTGGTCAGGGTATTTCATTCAGGGTTACGACGAGTGATGGGGTTACAAAAGATTTTAATAATGTGATGCCAAACAATTGGGGATTTGGACAGACTTTTGATGGAAAGATTAACTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0016020 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DPBB_1 Pollen_allerg_1

Protein Analysis

276

Amino Acids

30.49

Weight (kDa)

6.87

Isoelectric Point (pI)

22.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DPBB_1 PF03330 90 - 173 2.6e-21 Lytic transglycolase
Expansin_C PF01357 184 - 261 1.1e-25 Expansin C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 24, 305
AciI CCGC 1 cut(s) 272
AcsI RAATTY 1 cut(s) 388
AfiI CCNNNNNNNGG 1 cut(s) 146
AflIII ACRYGT 1 cut(s) 308
AgsI TTSAA 3 cut(s) 247, 295, 560
AleI CACNNNNGTG 1 cut(s) 352
AluBI AGCT 1 cut(s) 497
AluI AGCT 1 cut(s) 497
Alw21I GWGCWC 2 cut(s) 284, 342
Alw26I GTCTC 2 cut(s) 260, 639
Alw44I GTGCAC 1 cut(s) 338
ApaLI GTGCAC 1 cut(s) 338
ApeKI GCWGC 1 cut(s) 135
ApoI RAATTY 1 cut(s) 388
AseI ATTAAT 1 cut(s) 96
AspLEI GCGC 1 cut(s) 614
AspS9I GGNCC 1 cut(s) 693
AsuC2I CCSGG 2 cut(s) 363, 655
AsuHPI GGTGA 2 cut(s) 203, 248
AvaII GGWCC 1 cut(s) 693
BaeGI GKGCMC 1 cut(s) 342
BauI CACGAG 1 cut(s) 174
Bbv12I GWGCWC 2 cut(s) 284, 342
BbvI GCAGC 1 cut(s) 147
BccI CCATC 3 cut(s) 377, 743, 806
BciVI GTATCC 1 cut(s) 224
BcnI CCSGG 2 cut(s) 363, 655
BcoDI GTCTC 2 cut(s) 260, 639
BfaI CTAG 1 cut(s) 263
BfoI RGCGCY 1 cut(s) 615
BfuI GTATCC 1 cut(s) 224
BisI GCNGC 2 cut(s) 136, 273
BlsI GCNGC 2 cut(s) 137, 274
Bme1390I CCNGG 2 cut(s) 363, 655
Bme18I GGWCC 1 cut(s) 693
BmgT120I GGNCC 1 cut(s) 693
BmiI GGNNCC 2 cut(s) 223, 367
BmrFI CCNGG 2 cut(s) 363, 655
BmrI ACTGGG 2 cut(s) 503, 688
BmsI GCATC 3 cut(s) 160, 621, 768
BmuI ACTGGG 2 cut(s) 503, 688
BpmI CTGGAG 2 cut(s) 519, 636
BpuEI CTTGAG 1 cut(s) 461
BpuMI CCSGG 2 cut(s) 363, 655
BsaAI YACGTR 1 cut(s) 28
BsaJI CCNNGG 1 cut(s) 199
Bsc4I CCNNNNNNNGG 1 cut(s) 146
Bse1I ACTGG 6 cut(s) 509, 547, 683, 695, 703, 718
BseDI CCNNGG 1 cut(s) 199
BseLI CCNNNNNNNGG 1 cut(s) 146
BseNI ACTGG 6 cut(s) 509, 547, 683, 695, 703, 718
BseSI GKGCMC 1 cut(s) 342
BseXI GCAGC 1 cut(s) 147
BsiHKAI GWGCWC 2 cut(s) 284, 342
BsiSI CCGG 2 cut(s) 363, 654
BslFI GGGAC 2 cut(s) 491, 696
BslI CCNNNNNNNGG 1 cut(s) 146
BsmAI GTCTC 2 cut(s) 260, 639
BsmFI GGGAC 2 cut(s) 491, 696
Bsp1286I GDGCHC 2 cut(s) 284, 342
Bsp143I GATC 3 cut(s) 327, 480, 588
Bsp19I CCATGG 1 cut(s) 199
BspACI CCGC 1 cut(s) 272
BspLI GGNNCC 2 cut(s) 223, 367
BsrI ACTGG 6 cut(s) 509, 547, 683, 695, 703, 718
BssECI CCNNGG 1 cut(s) 199
BssMI GATC 3 cut(s) 327, 480, 588
BssNAI GTATAC 2 cut(s) 25, 306
BssSI CACGAG 1 cut(s) 174
BssT1I CCWWGG 1 cut(s) 199
Bst1107I GTATAC 2 cut(s) 25, 306
Bst2BI CACGAG 1 cut(s) 174
Bst4CI ACNGT 4 cut(s) 80, 158, 191, 236
Bst6I CTCTTC 1 cut(s) 296
BstBAI YACGTR 1 cut(s) 28
BstDEI CTNAG 1 cut(s) 10
BstDSI CCRYGG 1 cut(s) 199
BstH2I RGCGCY 1 cut(s) 615
BstHHI GCGC 1 cut(s) 614
BstKTI GATC 3 cut(s) 330, 483, 591
BstMAI GTCTC 2 cut(s) 260, 639
BstMBI GATC 3 cut(s) 327, 480, 588
BstMWI GCNNNNNNNGC 1 cut(s) 429
BstNSI RCATGY 1 cut(s) 312
BstSCI CCNGG 2 cut(s) 361, 653
BstSLI GKGCMC 1 cut(s) 342
BstSNI TACGTA 1 cut(s) 28
BstV1I GCAGC 1 cut(s) 147
BstXI CCANNNNNNTGG 1 cut(s) 789
BstZ17I GTATAC 2 cut(s) 25, 306
BsuI GTATCC 1 cut(s) 224
BtgI CCRYGG 1 cut(s) 199
BtgZI GCGATG 1 cut(s) 104
BtsIMutI CAGTG 1 cut(s) 696
CfoI GCGC 1 cut(s) 614
Cfr13I GGNCC 1 cut(s) 693
CviAII CATG 4 cut(s) 70, 200, 309, 331
CviJI RGCY 7 cut(s) 53, 107, 138, 224, 255, 317, 497
CviKI_1 RGCY 7 cut(s) 53, 107, 138, 224, 255, 317, 497
DdeI CTNAG 1 cut(s) 10
DpnI GATC 3 cut(s) 329, 482, 590
DpnII GATC 3 cut(s) 327, 480, 588
Eam1104I CTCTTC 1 cut(s) 296
EarI CTCTTC 1 cut(s) 296
Eco105I TACGTA 1 cut(s) 28
Eco130I CCWWGG 1 cut(s) 199
Eco32I GATATC 2 cut(s) 196, 622
Eco47I GGWCC 1 cut(s) 693
EcoRV GATATC 2 cut(s) 196, 622
EcoT14I CCWWGG 1 cut(s) 199
ErhI CCWWGG 1 cut(s) 199
FaeI CATG 4 cut(s) 73, 203, 312, 334
FalI AAGNNNNNCTT 1 cut(s) 809
FaqI GGGAC 2 cut(s) 491, 696
FatI CATG 4 cut(s) 69, 199, 308, 330
FblI GTMKAC 2 cut(s) 24, 305
Fnu4HI GCNGC 2 cut(s) 136, 273
Fsp4HI GCNGC 2 cut(s) 136, 273
FspBI CTAG 1 cut(s) 263
GlaI GCGC 1 cut(s) 613
GluI GCNGC 2 cut(s) 136, 273
GsuI CTGGAG 2 cut(s) 519, 636
HaeII RGCGCY 1 cut(s) 615
HapII CCGG 2 cut(s) 363, 654
HhaI GCGC 1 cut(s) 614
Hin1II CATG 4 cut(s) 73, 203, 312, 334
Hin6I GCGC 1 cut(s) 612
HinP1I GCGC 1 cut(s) 612
HinfI GANTC 1 cut(s) 150
HpaII CCGG 2 cut(s) 363, 654
HphI GGTGA 2 cut(s) 203, 248
Hpy166II GTNNAC 5 cut(s) 25, 306, 340, 359, 398
Hpy188III TCNNGA 3 cut(s) 263, 373, 478
Hpy8I GTNNAC 5 cut(s) 25, 306, 340, 359, 398
Hpy99I CGWCG 1 cut(s) 745
HpyAV CCTTC 2 cut(s) 293, 413
HpyCH4III ACNGT 4 cut(s) 80, 158, 191, 236
HpyCH4IV ACGT 2 cut(s) 27, 523
HpyCH4V TGCA 6 cut(s) 173, 340, 432, 536, 634, 670
HpyF10VI GCNNNNNNNGC 1 cut(s) 429
HpyF3I CTNAG 1 cut(s) 10
HpySE526I ACGT 2 cut(s) 27, 523
Hsp92II CATG 4 cut(s) 73, 203, 312, 334
HspAI GCGC 1 cut(s) 612
Kzo9I GATC 3 cut(s) 327, 480, 588
LmnI GCTCC 1 cut(s) 221
Lsp1109I GCAGC 1 cut(s) 147
LweI GCATC 3 cut(s) 160, 621, 768
MaeI CTAG 1 cut(s) 263
MaeII ACGT 2 cut(s) 27, 523
MaeIII GTNAC 4 cut(s) 74, 320, 736, 754
MalI GATC 3 cut(s) 329, 482, 590
MboI GATC 3 cut(s) 327, 480, 588
MboII GAAGA 1 cut(s) 283
MfeI CAATTG 1 cut(s) 787
MhlI GDGCHC 2 cut(s) 284, 342
MluCI AATT 3 cut(s) 388, 706, 787
MlyI GAGTC 1 cut(s) 144
MnlI CCTC 3 cut(s) 467, 485, 602
MseI TTAA 7 cut(s) 96, 513, 584, 639, 705, 768, 822
MslI CAYNNNNRTG 2 cut(s) 335, 352
MspA1I CMGCKG 1 cut(s) 272
MspI CCGG 2 cut(s) 363, 654
MspR9I CCNGG 2 cut(s) 363, 655
MunI CAATTG 1 cut(s) 787
MwoI GCNNNNNNNGC 1 cut(s) 429
NciI CCSGG 2 cut(s) 363, 655
NcoI CCATGG 1 cut(s) 199
NdeII GATC 3 cut(s) 327, 480, 588
NlaIII CATG 4 cut(s) 73, 203, 312, 334
NlaIV GGNNCC 2 cut(s) 223, 367
NspI RCATGY 1 cut(s) 312
OliI CACNNNNGTG 1 cut(s) 352
PciI ACATGT 1 cut(s) 308
PkrI GCNGC 2 cut(s) 137, 274
PleI GAGTC 1 cut(s) 144
PpsI GAGTC 1 cut(s) 144
Ppu21I YACGTR 1 cut(s) 28
PscI ACATGT 1 cut(s) 308
PshBI ATTAAT 1 cut(s) 96
PspN4I GGNNCC 2 cut(s) 223, 367
PspPI GGNCC 1 cut(s) 693
RseI CAYNNNNRTG 2 cut(s) 335, 352
SaqAI TTAA 7 cut(s) 96, 513, 584, 639, 705, 768, 822
SatI GCNGC 2 cut(s) 136, 273
Sau3AI GATC 3 cut(s) 327, 480, 588
Sau96I GGNCC 1 cut(s) 693
SchI GAGTC 1 cut(s) 144
ScrFI CCNGG 2 cut(s) 363, 655
SduI GDGCHC 2 cut(s) 284, 342
SetI ASST 4 cut(s) 30, 459, 499, 526
SfaNI GCATC 3 cut(s) 160, 621, 768
SinI GGWCC 1 cut(s) 693
SmiMI CAYNNNNRTG 2 cut(s) 335, 352
SmlI CTYRAG 1 cut(s) 476
SmoI CTYRAG 1 cut(s) 476
SnaBI TACGTA 1 cut(s) 28
Sse9I AATT 3 cut(s) 388, 706, 787
SsiI CCGC 1 cut(s) 272
SspMI CTAG 1 cut(s) 263
StyD4I CCNGG 2 cut(s) 361, 653
StyI CCWWGG 1 cut(s) 199
TaaI ACNGT 4 cut(s) 80, 158, 191, 236
TaiI ACGT 2 cut(s) 30, 526
TaqI TCGA 2 cut(s) 85, 153
TasI AATT 3 cut(s) 388, 706, 787
TauI GCSGC 1 cut(s) 275
Tru1I TTAA 7 cut(s) 96, 513, 584, 639, 705, 768, 822
Tru9I TTAA 7 cut(s) 96, 513, 584, 639, 705, 768, 822
TscAI CASTG 1 cut(s) 703
TseI GCWGC 1 cut(s) 135
TspDTI ATGAA 2 cut(s) 17, 717
TspGWI ACGGA 1 cut(s) 162
TspRI CASTG 1 cut(s) 703
VneI GTGCAC 1 cut(s) 338
VpaK11BI GGWCC 1 cut(s) 693
VspI ATTAAT 1 cut(s) 96
XapI RAATTY 1 cut(s) 388
XbaI TCTAGA 1 cut(s) 262
XceI RCATGY 1 cut(s) 312
XcmI CCANNNNNNNNNTGG 1 cut(s) 494
XmiI GTMKAC 2 cut(s) 24, 305
XspI CTAG 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.