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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 2 cut(s) | 81, 195 |
| AciI | CCGC | 1 cut(s) | 99 |
| AcoI | YGGCCR | 1 cut(s) | 3 |
| AcsI | RAATTY | 1 cut(s) | 163 |
| AfaI | GTAC | 1 cut(s) | 198 |
| AflIII | ACRYGT | 1 cut(s) | 193 |
| AjiI | CACGTC | 1 cut(s) | 124 |
| AoxI | GGCC | 3 cut(s) | 3, 7, 39 |
| ApoI | RAATTY | 1 cut(s) | 163 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 68 |
| AsuC2I | CCSGG | 1 cut(s) | 52 |
| AsuHPI | GGTGA | 1 cut(s) | 64 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 180, 213 |
| BbsI | GAAGAC | 1 cut(s) | 150 |
| BccI | CCATC | 2 cut(s) | 9, 31 |
| BcnI | CCSGG | 1 cut(s) | 52 |
| BglII | AGATCT | 1 cut(s) | 184 |
| Bme1390I | CCNGG | 1 cut(s) | 52 |
| BmgBI | CACGTC | 1 cut(s) | 124 |
| BmrFI | CCNGG | 1 cut(s) | 52 |
| BpiI | GAAGAC | 1 cut(s) | 150 |
| BpuMI | CCSGG | 1 cut(s) | 52 |
| Bse118I | RCCGGY | 1 cut(s) | 5 |
| Bsh1236I | CGCG | 2 cut(s) | 81, 195 |
| BshFI | GGCC | 3 cut(s) | 5, 9, 41 |
| BsiSI | CCGG | 2 cut(s) | 6, 52 |
| BsnI | GGCC | 3 cut(s) | 5, 9, 41 |
| Bsp143I | GATC | 1 cut(s) | 184 |
| BspACI | CCGC | 1 cut(s) | 99 |
| BspANI | GGCC | 3 cut(s) | 5, 9, 41 |
| BspFNI | CGCG | 2 cut(s) | 81, 195 |
| BsrFI | RCCGGY | 1 cut(s) | 5 |
| BssAI | RCCGGY | 1 cut(s) | 5 |
| BssMI | GATC | 1 cut(s) | 184 |
| Bst4CI | ACNGT | 1 cut(s) | 76 |
| BstC8I | GCNNGC | 1 cut(s) | 7 |
| BstDEI | CTNAG | 1 cut(s) | 11 |
| BstFNI | CGCG | 2 cut(s) | 81, 195 |
| BstKTI | GATC | 1 cut(s) | 187 |
| BstMBI | GATC | 1 cut(s) | 184 |
| BstSCI | CCNGG | 1 cut(s) | 50 |
| BstUI | CGCG | 2 cut(s) | 81, 195 |
| BstV2I | GAAGAC | 1 cut(s) | 150 |
| BstX2I | RGATCY | 1 cut(s) | 184 |
| BstYI | RGATCY | 1 cut(s) | 184 |
| BsuRI | GGCC | 3 cut(s) | 5, 9, 41 |
| BtrI | CACGTC | 1 cut(s) | 124 |
| Cac8I | GCNNGC | 1 cut(s) | 7 |
| Cfr10I | RCCGGY | 1 cut(s) | 5 |
| CseI | GACGC | 1 cut(s) | 70 |
| Csp6I | GTAC | 1 cut(s) | 197 |
| CviAII | CATG | 1 cut(s) | 119 |
| CviJI | RGCY | 3 cut(s) | 5, 9, 41 |
| CviKI_1 | RGCY | 3 cut(s) | 5, 9, 41 |
| CviQI | GTAC | 1 cut(s) | 197 |
| DdeI | CTNAG | 1 cut(s) | 11 |
| DpnI | GATC | 1 cut(s) | 186 |
| DpnII | GATC | 1 cut(s) | 184 |
| EaeI | YGGCCR | 1 cut(s) | 3 |
| FaeI | CATG | 1 cut(s) | 122 |
| FaiI | YATR | 4 cut(s) | 120, 141, 149, 155 |
| FatI | CATG | 1 cut(s) | 118 |
| FseI | GGCCGGCC | 1 cut(s) | 9 |
| HaeIII | GGCC | 3 cut(s) | 5, 9, 41 |
| HapII | CCGG | 2 cut(s) | 6, 52 |
| HgaI | GACGC | 1 cut(s) | 70 |
| Hin1II | CATG | 1 cut(s) | 122 |
| HinfI | GANTC | 3 cut(s) | 64, 241, 270 |
| HpaII | CCGG | 2 cut(s) | 6, 52 |
| HphI | GGTGA | 1 cut(s) | 64 |
| Hpy188I | TCNGA | 1 cut(s) | 275 |
| Hpy188III | TCNNGA | 1 cut(s) | 61 |
| Hpy99I | CGWCG | 1 cut(s) | 86 |
| HpyAV | CCTTC | 1 cut(s) | 52 |
| HpyCH4III | ACNGT | 1 cut(s) | 76 |
| HpyCH4IV | ACGT | 1 cut(s) | 123 |
| HpyF3I | CTNAG | 1 cut(s) | 11 |
| HpySE526I | ACGT | 1 cut(s) | 123 |
| Hsp92II | CATG | 1 cut(s) | 122 |
| KroI | GCCGGC | 1 cut(s) | 5 |
| KroNI | GCCGGC | 1 cut(s) | 7 |
| Kzo9I | GATC | 1 cut(s) | 184 |
| LpnPI | CCDG | 4 cut(s) | 19, 23, 46, 65 |
| MaeII | ACGT | 1 cut(s) | 123 |
| MaeIII | GTNAC | 1 cut(s) | 168 |
| MalI | GATC | 1 cut(s) | 186 |
| MboI | GATC | 1 cut(s) | 184 |
| MboII | GAAGA | 4 cut(s) | 155, 194, 236, 238 |
| MflI | RGATCY | 1 cut(s) | 184 |
| MluCI | AATT | 1 cut(s) | 163 |
| MluI | ACGCGT | 1 cut(s) | 193 |
| MmeI | TCCRAC | 2 cut(s) | 218, 229 |
| MnlI | CCTC | 2 cut(s) | 102, 216 |
| MroNI | GCCGGC | 1 cut(s) | 5 |
| MroXI | GAANNNNTTC | 1 cut(s) | 68 |
| MslI | CAYNNNNRTG | 1 cut(s) | 177 |
| MspA1I | CMGCKG | 1 cut(s) | 99 |
| MspI | CCGG | 2 cut(s) | 6, 52 |
| MspR9I | CCNGG | 1 cut(s) | 52 |
| MvnI | CGCG | 2 cut(s) | 81, 195 |
| NaeI | GCCGGC | 1 cut(s) | 7 |
| NciI | CCSGG | 1 cut(s) | 52 |
| NdeII | GATC | 1 cut(s) | 184 |
| NgoMIV | GCCGGC | 1 cut(s) | 5 |
| NlaIII | CATG | 1 cut(s) | 122 |
| PdiI | GCCGGC | 1 cut(s) | 7 |
| PdmI | GAANNNNTTC | 1 cut(s) | 68 |
| PfeI | GAWTC | 3 cut(s) | 64, 241, 270 |
| PsuI | RGATCY | 1 cut(s) | 184 |
| RigI | GGCCGGCC | 1 cut(s) | 9 |
| RsaI | GTAC | 1 cut(s) | 198 |
| RsaNI | GTAC | 1 cut(s) | 197 |
| RseI | CAYNNNNRTG | 1 cut(s) | 177 |
| Sau3AI | GATC | 1 cut(s) | 184 |
| ScrFI | CCNGG | 1 cut(s) | 52 |
| SetI | ASST | 3 cut(s) | 113, 126, 235 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 177 |
| Sse9I | AATT | 1 cut(s) | 163 |
| SsiI | CCGC | 1 cut(s) | 99 |
| StyD4I | CCNGG | 1 cut(s) | 50 |
| TaaI | ACNGT | 1 cut(s) | 76 |
| TaiI | ACGT | 1 cut(s) | 126 |
| TaqI | TCGA | 1 cut(s) | 91 |
| TasI | AATT | 1 cut(s) | 163 |
| TfiI | GAWTC | 3 cut(s) | 64, 241, 270 |
| TspDTI | ATGAA | 2 cut(s) | 156, 195 |
| XapI | RAATTY | 1 cut(s) | 163 |
| XmnI | GAANNNNTTC | 1 cut(s) | 68 |