MD15G1072300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001322
Physical Location & Seq
Reverse (-)
4942403 .. 4943375
973 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1072300.v1.1.491

Sequence Viewer

Length: 267 bp
ATGAAGTTCCCGATGCGGAAAATCTGGAGCCGGGTCGCAACCCACCTCGGCATCCACAAATCGGGGCTACCGAAGCTAAGACATGATATAAGAACATGCGAGTACGAGGACGTGCATGTGATGTGGAGGATGCTCAACCCTAATGACAGATCAGAGACCATTCAATTGGGAGGAGGAGGAGGAGGAGGAGAAACGGCCAAGAAGAAACGTTTTTCCAATTTGTTCGATTGGATTAAACGAACTCCATCGGTTTGCCGTGCCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

89

Amino Acids

10.18

Weight (kDa)

10.29

Isoelectric Point (pI)

45.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 16
AclI AACGTT 1 cut(s) 208
AcoI YGGCCR 1 cut(s) 195
AfaI GTAC 1 cut(s) 104
AfiI CCNNNNNNNGG 1 cut(s) 61
AgsI TTSAA 1 cut(s) 164
AjiI CACGTC 1 cut(s) 112
AluBI AGCT 1 cut(s) 76
AluI AGCT 1 cut(s) 76
Alw26I GTCTC 1 cut(s) 149
AoxI GGCC 1 cut(s) 195
AsuC2I CCSGG 1 cut(s) 32
BccI CCATC 1 cut(s) 253
BceAI ACGGC 2 cut(s) 210, 240
BcnI CCSGG 1 cut(s) 32
BcoDI GTCTC 1 cut(s) 149
Bme1390I CCNGG 1 cut(s) 32
BmgBI CACGTC 1 cut(s) 112
BmiI GGNNCC 1 cut(s) 29
BmrFI CCNGG 1 cut(s) 32
BmsI GCATC 3 cut(s) 3, 60, 120
BpmI CTGGAG 1 cut(s) 46
BpuMI CCSGG 1 cut(s) 32
BsaI GGTCTC 1 cut(s) 149
BsaJI CCNNGG 1 cut(s) 46
Bsc4I CCNNNNNNNGG 1 cut(s) 61
BseDI CCNNGG 1 cut(s) 46
BseGI GGATG 2 cut(s) 51, 135
BseLI CCNNNNNNNGG 1 cut(s) 61
BseRI GAGGAG 6 cut(s) 186, 189, 192, 195, 198, 201
BshFI GGCC 1 cut(s) 197
BsiSI CCGG 1 cut(s) 31
BslI CCNNNNNNNGG 1 cut(s) 61
BsmAI GTCTC 1 cut(s) 149
BsnI GGCC 1 cut(s) 197
Bso31I GGTCTC 1 cut(s) 149
Bsp143I GATC 1 cut(s) 149
BspACI CCGC 1 cut(s) 16
BspANI GGCC 1 cut(s) 197
BspLI GGNNCC 1 cut(s) 29
BspTNI GGTCTC 1 cut(s) 149
BssECI CCNNGG 1 cut(s) 46
BssMI GATC 1 cut(s) 149
BstDEI CTNAG 1 cut(s) 77
BstF5I GGATG 2 cut(s) 51, 135
BstKTI GATC 1 cut(s) 152
BstMAI GTCTC 1 cut(s) 149
BstMBI GATC 1 cut(s) 149
BstMWI GCNNNNNNNGC 1 cut(s) 73
BstNSI RCATGY 2 cut(s) 99, 119
BstSCI CCNGG 1 cut(s) 30
BstXI CCANNNNNNTGG 1 cut(s) 166
BsuRI GGCC 1 cut(s) 197
BtrI CACGTC 1 cut(s) 112
BtsCI GGATG 2 cut(s) 51, 135
Csp6I GTAC 1 cut(s) 103
CviAII CATG 3 cut(s) 83, 96, 116
CviJI RGCY 4 cut(s) 30, 67, 76, 197
CviKI_1 RGCY 4 cut(s) 30, 67, 76, 197
CviQI GTAC 1 cut(s) 103
DdeI CTNAG 1 cut(s) 77
DpnI GATC 1 cut(s) 151
DpnII GATC 1 cut(s) 149
EaeI YGGCCR 1 cut(s) 195
Eco31I GGTCTC 1 cut(s) 149
FaeI CATG 3 cut(s) 86, 99, 119
FaiI YATR 4 cut(s) 84, 89, 97, 117
FatI CATG 3 cut(s) 82, 95, 115
FokI GGATG 2 cut(s) 38, 142
GsuI CTGGAG 1 cut(s) 46
HaeIII GGCC 1 cut(s) 197
HapII CCGG 1 cut(s) 31
Hin1II CATG 3 cut(s) 86, 99, 119
HpaII CCGG 1 cut(s) 31
Hpy188I TCNGA 2 cut(s) 154, 266
Hpy188III TCNNGA 2 cut(s) 10, 25
HpyCH4IV ACGT 2 cut(s) 111, 208
HpyCH4V TGCA 1 cut(s) 115
HpyF10VI GCNNNNNNNGC 1 cut(s) 73
HpyF3I CTNAG 1 cut(s) 77
HpySE526I ACGT 2 cut(s) 111, 208
Hsp92II CATG 3 cut(s) 86, 99, 119
Kzo9I GATC 1 cut(s) 149
LmnI GCTCC 1 cut(s) 27
LpnPI CCDG 2 cut(s) 10, 44
LweI GCATC 3 cut(s) 3, 60, 120
MaeII ACGT 2 cut(s) 111, 208
MalI GATC 1 cut(s) 151
MboI GATC 1 cut(s) 149
MboII GAAGA 1 cut(s) 214
MfeI CAATTG 1 cut(s) 164
MluCI AATT 2 cut(s) 164, 217
MnlI CCTC 9 cut(s) 56, 100, 120, 164, 167, 170, 173, 176, 179
MseI TTAA 1 cut(s) 234
MspI CCGG 1 cut(s) 31
MspR9I CCNGG 1 cut(s) 32
MunI CAATTG 1 cut(s) 164
MwoI GCNNNNNNNGC 1 cut(s) 73
NciI CCSGG 1 cut(s) 32
NdeII GATC 1 cut(s) 149
NlaIII CATG 3 cut(s) 86, 99, 119
NlaIV GGNNCC 1 cut(s) 29
NmeAIII GCCGAG 1 cut(s) 27
NspI RCATGY 2 cut(s) 99, 119
PcsI WCGNNNNNNNCGW 1 cut(s) 68
Psp1406I AACGTT 1 cut(s) 208
PspN4I GGNNCC 1 cut(s) 29
RsaI GTAC 1 cut(s) 104
RsaNI GTAC 1 cut(s) 103
SaqAI TTAA 1 cut(s) 234
Sau3AI GATC 1 cut(s) 149
ScrFI CCNGG 1 cut(s) 32
SetI ASST 4 cut(s) 48, 78, 114, 211
SfaNI GCATC 3 cut(s) 3, 60, 120
Sse9I AATT 2 cut(s) 164, 217
SsiI CCGC 1 cut(s) 16
StyD4I CCNGG 1 cut(s) 30
TaiI ACGT 2 cut(s) 114, 211
TaqI TCGA 1 cut(s) 225
TasI AATT 2 cut(s) 164, 217
Tru1I TTAA 1 cut(s) 234
Tru9I TTAA 1 cut(s) 234
TspDTI ATGAA 1 cut(s) 17
XceI RCATGY 2 cut(s) 99, 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.