MD15G1072200.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0001322
Physical Location & Seq
Reverse (-)
4937355 .. 4941591
4237 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1072200.v1.1.491

Sequence Viewer

Length: 357 bp
ATGGGACACTCGAACGGATGGAACTCTCACCCCAAGAACTACGGCCCCGGTTCCCGCACTTGCCGAGTGTGTGGAAACCCTCATGGGTTGATCCGGAAGTATGCGCTGATGTGCTGCAGGCAGTGCTTCCGCAGCAATGCCAAGGAGATTGGCTTCATCAAGCTTGGACGACCGAAGCTAAGACATGATATAAGAACATGCGAGTACGAGGACGTGCATGTGATGTGGAGGATGCTCAACCCTAATGACAGATCAGAGACCATTCAATTGGGAGGAGGAGTAACGCCCAAGAGGAAACGTTTTTCCAATTTGTTCGATTGGATTAAACGAGCTCCATCGGTTTGCCGTGCCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

119

Amino Acids

13.66

Weight (kDa)

10.13

Isoelectric Point (pI)

39.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S14 PF00253 7 - 54 1.7e-13 Ribosomal protein S14p/S29e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 93
AciI CCGC 2 cut(s) 55, 130
AclI AACGTT 1 cut(s) 298
AclWI GGATC 1 cut(s) 85
AfaI GTAC 1 cut(s) 206
AgsI TTSAA 1 cut(s) 266
AjiI CACGTC 1 cut(s) 214
AluBI AGCT 3 cut(s) 163, 178, 332
AluI AGCT 3 cut(s) 163, 178, 332
Alw21I GWGCWC 1 cut(s) 334
Alw26I GTCTC 1 cut(s) 251
AlwI GGATC 1 cut(s) 85
Aor13HI TCCGGA 1 cut(s) 93
AoxI GGCC 1 cut(s) 43
ApeKI GCWGC 2 cut(s) 114, 132
AspLEI GCGC 1 cut(s) 106
AspS9I GGNCC 1 cut(s) 44
AsuC2I CCSGG 1 cut(s) 48
AsuHPI GGTGA 1 cut(s) 20
BanII GRGCYC 1 cut(s) 334
Bbv12I GWGCWC 1 cut(s) 334
BbvI GCAGC 2 cut(s) 101, 144
BccI CCATC 2 cut(s) 12, 343
BceAI ACGGC 2 cut(s) 58, 330
BcnI CCSGG 1 cut(s) 48
BcoDI GTCTC 1 cut(s) 251
BfmI CTRYAG 1 cut(s) 115
BisI GCNGC 2 cut(s) 115, 133
BlsI GCNGC 2 cut(s) 116, 134
Bme1390I CCNGG 1 cut(s) 48
BmgBI CACGTC 1 cut(s) 214
BmgT120I GGNCC 1 cut(s) 44
BmiI GGNNCC 2 cut(s) 46, 52
BmrFI CCNGG 1 cut(s) 48
BmsI GCATC 1 cut(s) 222
BpuMI CCSGG 1 cut(s) 48
BsaI GGTCTC 1 cut(s) 251
BsaJI CCNNGG 2 cut(s) 46, 141
BsaWI WCCGGW 1 cut(s) 93
BsaXI ACNNNNNCTCC 2 cut(s) 264, 294
Bse3DI GCAATG 1 cut(s) 142
BseAI TCCGGA 1 cut(s) 93
BseDI CCNNGG 2 cut(s) 46, 141
BseGI GGATG 2 cut(s) 23, 237
BseMI GCAATG 1 cut(s) 142
BseRI GAGGAG 2 cut(s) 288, 291
BseXI GCAGC 2 cut(s) 101, 144
Bsh1285I CGRYCG 1 cut(s) 173
BshFI GGCC 1 cut(s) 45
BsiEI CGRYCG 1 cut(s) 173
BsiHKAI GWGCWC 1 cut(s) 334
BsiSI CCGG 2 cut(s) 48, 94
BslFI GGGAC 1 cut(s) 18
BsmAI GTCTC 1 cut(s) 251
BsmFI GGGAC 1 cut(s) 18
BsnI GGCC 1 cut(s) 45
Bso31I GGTCTC 1 cut(s) 251
Bsp1286I GDGCHC 1 cut(s) 334
Bsp13I TCCGGA 1 cut(s) 93
Bsp143I GATC 2 cut(s) 90, 251
BspACI CCGC 2 cut(s) 55, 130
BspANI GGCC 1 cut(s) 45
BspEI TCCGGA 1 cut(s) 93
BspLI GGNNCC 2 cut(s) 46, 52
BspMAI CTGCAG 1 cut(s) 119
BspPI GGATC 1 cut(s) 85
BspTNI GGTCTC 1 cut(s) 251
BsrDI GCAATG 1 cut(s) 142
BssECI CCNNGG 2 cut(s) 46, 141
BssMI GATC 2 cut(s) 90, 251
BssT1I CCWWGG 1 cut(s) 141
BstAPI GCANNNNNTGC 1 cut(s) 123
BstC8I GCNNGC 1 cut(s) 119
BstDEI CTNAG 1 cut(s) 179
BstF5I GGATG 2 cut(s) 23, 237
BstHHI GCGC 1 cut(s) 106
BstKTI GATC 2 cut(s) 93, 254
BstMAI GTCTC 1 cut(s) 251
BstMBI GATC 2 cut(s) 90, 251
BstMCI CGRYCG 1 cut(s) 173
BstMWI GCNNNNNNNGC 2 cut(s) 123, 132
BstNSI RCATGY 2 cut(s) 201, 221
BstSCI CCNGG 1 cut(s) 46
BstSFI CTRYAG 1 cut(s) 115
BstV1I GCAGC 2 cut(s) 101, 144
BstXI CCANNNNNNTGG 1 cut(s) 268
BsuRI GGCC 1 cut(s) 45
BtrI CACGTC 1 cut(s) 214
BtsCI GGATG 2 cut(s) 23, 237
BtsI GCAGTG 1 cut(s) 128
BtsIMutI CAGTG 1 cut(s) 128
Cac8I GCNNGC 1 cut(s) 119
CfoI GCGC 1 cut(s) 106
Cfr13I GGNCC 1 cut(s) 44
Csp6I GTAC 1 cut(s) 205
CviAII CATG 4 cut(s) 83, 185, 198, 218
CviJI RGCY 5 cut(s) 45, 153, 163, 178, 332
CviKI_1 RGCY 5 cut(s) 45, 153, 163, 178, 332
CviQI GTAC 1 cut(s) 205
DdeI CTNAG 1 cut(s) 179
DpnI GATC 2 cut(s) 92, 253
DpnII GATC 2 cut(s) 90, 251
Ecl136II GAGCTC 1 cut(s) 332
Eco130I CCWWGG 1 cut(s) 141
Eco24I GRGCYC 1 cut(s) 334
Eco31I GGTCTC 1 cut(s) 251
Eco53kI GAGCTC 1 cut(s) 332
EcoICRI GAGCTC 1 cut(s) 332
EcoT14I CCWWGG 1 cut(s) 141
EcoT38I GRGCYC 1 cut(s) 334
ErhI CCWWGG 1 cut(s) 141
FaeI CATG 4 cut(s) 86, 188, 201, 221
FaiI YATR 6 cut(s) 84, 102, 186, 191, 199, 219
FaqI GGGAC 1 cut(s) 18
FatI CATG 4 cut(s) 82, 184, 197, 217
FauI CCCGC 1 cut(s) 62
Fnu4HI GCNGC 2 cut(s) 115, 133
FokI GGATG 2 cut(s) 30, 244
FriOI GRGCYC 1 cut(s) 334
Fsp4HI GCNGC 2 cut(s) 115, 133
GlaI GCGC 1 cut(s) 105
GluI GCNGC 2 cut(s) 115, 133
HaeIII GGCC 1 cut(s) 45
HapII CCGG 2 cut(s) 48, 94
HhaI GCGC 1 cut(s) 106
Hin1II CATG 4 cut(s) 86, 188, 201, 221
Hin6I GCGC 1 cut(s) 104
HinP1I GCGC 1 cut(s) 104
HindIII AAGCTT 1 cut(s) 161
HpaII CCGG 2 cut(s) 48, 94
HphI GGTGA 1 cut(s) 20
Hpy188I TCNGA 2 cut(s) 256, 356
Hpy188III TCNNGA 1 cut(s) 94
HpyCH4IV ACGT 2 cut(s) 213, 298
HpyCH4V TGCA 2 cut(s) 117, 217
HpyF10VI GCNNNNNNNGC 2 cut(s) 123, 132
HpyF3I CTNAG 1 cut(s) 179
HpySE526I ACGT 2 cut(s) 213, 298
Hsp92II CATG 4 cut(s) 86, 188, 201, 221
HspAI GCGC 1 cut(s) 104
Kpn2I TCCGGA 1 cut(s) 93
Kzo9I GATC 2 cut(s) 90, 251
LmnI GCTCC 1 cut(s) 337
LpnPI CCDG 3 cut(s) 61, 103, 107
Lsp1109I GCAGC 2 cut(s) 101, 144
LweI GCATC 1 cut(s) 222
MaeII ACGT 2 cut(s) 213, 298
MaeIII GTNAC 1 cut(s) 280
MalI GATC 2 cut(s) 92, 253
MboI GATC 2 cut(s) 90, 251
MfeI CAATTG 1 cut(s) 266
MhlI GDGCHC 1 cut(s) 334
MluCI AATT 2 cut(s) 266, 307
MnlI CCTC 6 cut(s) 90, 202, 222, 266, 269, 285
MroI TCCGGA 1 cut(s) 93
MseI TTAA 1 cut(s) 324
MspI CCGG 2 cut(s) 48, 94
MspR9I CCNGG 1 cut(s) 48
MunI CAATTG 1 cut(s) 266
MwoI GCNNNNNNNGC 2 cut(s) 123, 132
NciI CCSGG 1 cut(s) 48
NdeII GATC 2 cut(s) 90, 251
NlaIII CATG 4 cut(s) 86, 188, 201, 221
NlaIV GGNNCC 2 cut(s) 46, 52
NmeAIII GCCGAG 1 cut(s) 89
NspI RCATGY 2 cut(s) 201, 221
PkrI GCNGC 2 cut(s) 116, 134
Psp124BI GAGCTC 1 cut(s) 334
Psp1406I AACGTT 1 cut(s) 298
PspN4I GGNNCC 2 cut(s) 46, 52
PspPI GGNCC 1 cut(s) 44
PstI CTGCAG 1 cut(s) 119
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
SacI GAGCTC 1 cut(s) 334
SaqAI TTAA 1 cut(s) 324
SatI GCNGC 2 cut(s) 115, 133
Sau3AI GATC 2 cut(s) 90, 251
Sau96I GGNCC 1 cut(s) 44
ScrFI CCNGG 1 cut(s) 48
SduI GDGCHC 1 cut(s) 334
SetI ASST 5 cut(s) 165, 180, 216, 301, 334
SfaNI GCATC 1 cut(s) 222
SfcI CTRYAG 1 cut(s) 115
Sse9I AATT 2 cut(s) 266, 307
SsiI CCGC 2 cut(s) 55, 130
SstI GAGCTC 1 cut(s) 334
StyD4I CCNGG 1 cut(s) 46
StyI CCWWGG 1 cut(s) 141
TaiI ACGT 2 cut(s) 216, 301
TaqI TCGA 2 cut(s) 11, 315
TaqII GACCGA 1 cut(s) 187
TasI AATT 2 cut(s) 266, 307
Tru1I TTAA 1 cut(s) 324
Tru9I TTAA 1 cut(s) 324
TscAI CASTG 1 cut(s) 128
TseI GCWGC 2 cut(s) 114, 132
TspDTI ATGAA 1 cut(s) 145
TspGWI ACGGA 1 cut(s) 30
TspRI CASTG 1 cut(s) 128
XceI RCATGY 2 cut(s) 201, 221
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.