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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 62 |
| AciI | CCGC | 1 cut(s) | 60 |
| AfaI | GTAC | 1 cut(s) | 175 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 121 |
| AflII | CTTAAG | 1 cut(s) | 32 |
| AflIII | ACRYGT | 1 cut(s) | 170 |
| AhlI | ACTAGT | 1 cut(s) | 148 |
| AoxI | GGCC | 1 cut(s) | 156 |
| ApeKI | GCWGC | 1 cut(s) | 10 |
| AspS9I | GGNCC | 1 cut(s) | 157 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 166, 198 |
| BccI | CCATC | 2 cut(s) | 26, 208 |
| BcuI | ACTAGT | 1 cut(s) | 148 |
| BfaI | CTAG | 1 cut(s) | 149 |
| BfrI | CTTAAG | 1 cut(s) | 32 |
| BisI | GCNGC | 1 cut(s) | 11 |
| BlsI | GCNGC | 1 cut(s) | 12 |
| BmgT120I | GGNCC | 1 cut(s) | 157 |
| BsaAI | YACGTR | 1 cut(s) | 173 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 121 |
| BseGI | GGATG | 2 cut(s) | 106, 130 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 121 |
| BseRI | GAGGAG | 1 cut(s) | 18 |
| BsgI | GTGCAG | 1 cut(s) | 162 |
| BshFI | GGCC | 1 cut(s) | 158 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 121 |
| BsnI | GGCC | 1 cut(s) | 158 |
| BspACI | CCGC | 1 cut(s) | 60 |
| BspANI | GGCC | 1 cut(s) | 158 |
| BspTI | CTTAAG | 1 cut(s) | 32 |
| BsrBI | CCGCTC | 1 cut(s) | 62 |
| Bst6I | CTCTTC | 1 cut(s) | 70 |
| BstAFI | CTTAAG | 1 cut(s) | 32 |
| BstBAI | YACGTR | 1 cut(s) | 173 |
| BstF5I | GGATG | 2 cut(s) | 106, 130 |
| BstNSI | RCATGY | 1 cut(s) | 134 |
| BsuRI | GGCC | 1 cut(s) | 158 |
| BtsCI | GGATG | 2 cut(s) | 106, 130 |
| Cfr13I | GGNCC | 1 cut(s) | 157 |
| Csp6I | GTAC | 1 cut(s) | 174 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 132, 167 |
| CviAII | CATG | 3 cut(s) | 131, 161, 210 |
| CviJI | RGCY | 2 cut(s) | 10, 158 |
| CviKI_1 | RGCY | 2 cut(s) | 10, 158 |
| CviQI | GTAC | 1 cut(s) | 174 |
| Eam1104I | CTCTTC | 1 cut(s) | 70 |
| EarI | CTCTTC | 1 cut(s) | 70 |
| FaeI | CATG | 3 cut(s) | 134, 164, 213 |
| FaiI | YATR | 5 cut(s) | 96, 132, 162, 168, 211 |
| FatI | CATG | 3 cut(s) | 130, 160, 209 |
| Fnu4HI | GCNGC | 1 cut(s) | 11 |
| FokI | GGATG | 2 cut(s) | 113, 137 |
| Fsp4HI | GCNGC | 1 cut(s) | 11 |
| FspBI | CTAG | 1 cut(s) | 149 |
| GluI | GCNGC | 1 cut(s) | 11 |
| HaeIII | GGCC | 1 cut(s) | 158 |
| Hin1II | CATG | 3 cut(s) | 134, 164, 213 |
| Hpy188I | TCNGA | 1 cut(s) | 66 |
| HpyAV | CCTTC | 1 cut(s) | 192 |
| HpyCH4IV | ACGT | 1 cut(s) | 172 |
| HpyCH4V | TGCA | 4 cut(s) | 13, 130, 164, 179 |
| HpySE526I | ACGT | 1 cut(s) | 172 |
| Hsp92II | CATG | 3 cut(s) | 134, 164, 213 |
| LmnI | GCTCC | 1 cut(s) | 67 |
| LpnPI | CCDG | 2 cut(s) | 124, 149 |
| MaeI | CTAG | 1 cut(s) | 149 |
| MaeII | ACGT | 1 cut(s) | 172 |
| MbiI | CCGCTC | 1 cut(s) | 62 |
| MboII | GAAGA | 4 cut(s) | 13, 49, 79, 87 |
| MnlI | CCTC | 5 cut(s) | 39, 71, 91, 115, 148 |
| MseI | TTAA | 1 cut(s) | 33 |
| MspCI | CTTAAG | 1 cut(s) | 32 |
| NlaIII | CATG | 3 cut(s) | 134, 164, 213 |
| NspI | RCATGY | 1 cut(s) | 134 |
| PkrI | GCNGC | 1 cut(s) | 12 |
| Ppu21I | YACGTR | 1 cut(s) | 173 |
| PspPI | GGNCC | 1 cut(s) | 157 |
| RsaI | GTAC | 1 cut(s) | 175 |
| RsaNI | GTAC | 1 cut(s) | 174 |
| SaqAI | TTAA | 1 cut(s) | 33 |
| SatI | GCNGC | 1 cut(s) | 11 |
| Sau96I | GGNCC | 1 cut(s) | 157 |
| SetI | ASST | 2 cut(s) | 175, 184 |
| SgeI | CNNG | 7 cut(s) | 123, 128, 143, 148, 161, 173, 183 |
| SmlI | CTYRAG | 1 cut(s) | 32 |
| SmoI | CTYRAG | 1 cut(s) | 32 |
| SpeI | ACTAGT | 1 cut(s) | 148 |
| SsiI | CCGC | 1 cut(s) | 60 |
| SspMI | CTAG | 1 cut(s) | 149 |
| TaiI | ACGT | 1 cut(s) | 175 |
| Tru1I | TTAA | 1 cut(s) | 33 |
| Tru9I | TTAA | 1 cut(s) | 33 |
| TseI | GCWGC | 1 cut(s) | 10 |
| Vha464I | CTTAAG | 1 cut(s) | 32 |
| XceI | RCATGY | 1 cut(s) | 134 |
| XspI | CTAG | 1 cut(s) | 149 |