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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 63 |
| AciI | CCGC | 2 cut(s) | 34, 61 |
| AdeI | CACNNNGTG | 1 cut(s) | 106 |
| AjiI | CACGTC | 1 cut(s) | 130 |
| AleI | CACNNNNGTG | 1 cut(s) | 259 |
| AluBI | AGCT | 3 cut(s) | 41, 94, 192 |
| AluI | AGCT | 3 cut(s) | 41, 94, 192 |
| Alw26I | GTCTC | 1 cut(s) | 164 |
| ApeKI | GCWGC | 1 cut(s) | 94 |
| AsuC2I | CCSGG | 2 cut(s) | 50, 82 |
| BbvI | GCAGC | 1 cut(s) | 81 |
| BceAI | ACGGC | 1 cut(s) | 249 |
| BcnI | CCSGG | 2 cut(s) | 50, 82 |
| BcoDI | GTCTC | 1 cut(s) | 164 |
| BisI | GCNGC | 1 cut(s) | 95 |
| BlsI | GCNGC | 1 cut(s) | 96 |
| Bme1390I | CCNGG | 2 cut(s) | 50, 82 |
| BmgBI | CACGTC | 1 cut(s) | 130 |
| BmrFI | CCNGG | 2 cut(s) | 50, 82 |
| BmsI | GCATC | 2 cut(s) | 21, 204 |
| BpuEI | CTTGAG | 1 cut(s) | 178 |
| BpuMI | CCSGG | 2 cut(s) | 50, 82 |
| BseXI | GCAGC | 1 cut(s) | 81 |
| Bsh1236I | CGCG | 1 cut(s) | 63 |
| BsiSI | CCGG | 2 cut(s) | 49, 81 |
| BsmAI | GTCTC | 1 cut(s) | 164 |
| BspACI | CCGC | 2 cut(s) | 34, 61 |
| BspFNI | CGCG | 1 cut(s) | 63 |
| BstC8I | GCNNGC | 1 cut(s) | 115 |
| BstFNI | CGCG | 1 cut(s) | 63 |
| BstMAI | GTCTC | 1 cut(s) | 164 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 91 |
| BstSCI | CCNGG | 2 cut(s) | 48, 80 |
| BstUI | CGCG | 1 cut(s) | 63 |
| BstV1I | GCAGC | 1 cut(s) | 81 |
| BtrI | CACGTC | 1 cut(s) | 130 |
| Cac8I | GCNNGC | 1 cut(s) | 115 |
| CseI | GACGC | 1 cut(s) | 52 |
| CviJI | RGCY | 6 cut(s) | 41, 85, 94, 113, 166, 192 |
| CviKI_1 | RGCY | 6 cut(s) | 41, 85, 94, 113, 166, 192 |
| DraIII | CACNNNGTG | 1 cut(s) | 106 |
| FaiI | YATR | 3 cut(s) | 123, 149, 260 |
| FauI | CCCGC | 1 cut(s) | 68 |
| Fnu4HI | GCNGC | 1 cut(s) | 95 |
| Fsp4HI | GCNGC | 1 cut(s) | 95 |
| GluI | GCNGC | 1 cut(s) | 95 |
| HapII | CCGG | 2 cut(s) | 49, 81 |
| HgaI | GACGC | 1 cut(s) | 52 |
| HinfI | GANTC | 1 cut(s) | 69 |
| HpaII | CCGG | 2 cut(s) | 49, 81 |
| Hpy188I | TCNGA | 3 cut(s) | 30, 68, 74 |
| Hpy99I | CGWCG | 1 cut(s) | 68 |
| HpyAV | CCTTC | 2 cut(s) | 103, 228 |
| HpyCH4IV | ACGT | 1 cut(s) | 129 |
| HpyCH4V | TGCA | 1 cut(s) | 225 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 91 |
| HpySE526I | ACGT | 1 cut(s) | 129 |
| LpnPI | CCDG | 3 cut(s) | 62, 94, 180 |
| Lsp1109I | GCAGC | 1 cut(s) | 81 |
| LweI | GCATC | 2 cut(s) | 21, 204 |
| MaeII | ACGT | 1 cut(s) | 129 |
| MboII | GAAGA | 5 cut(s) | 211, 214, 217, 220, 223 |
| MluCI | AATT | 1 cut(s) | 226 |
| MlyI | GAGTC | 1 cut(s) | 63 |
| MmeI | TCCRAC | 1 cut(s) | 46 |
| MnlI | CCTC | 1 cut(s) | 118 |
| MseI | TTAA | 2 cut(s) | 45, 243 |
| MslI | CAYNNNNRTG | 1 cut(s) | 259 |
| MspI | CCGG | 2 cut(s) | 49, 81 |
| MspR9I | CCNGG | 2 cut(s) | 50, 82 |
| MvnI | CGCG | 1 cut(s) | 63 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 91 |
| NciI | CCSGG | 2 cut(s) | 50, 82 |
| OliI | CACNNNNGTG | 1 cut(s) | 259 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 60 |
| PkrI | GCNGC | 1 cut(s) | 96 |
| PleI | GAGTC | 1 cut(s) | 63 |
| PpsI | GAGTC | 1 cut(s) | 63 |
| RseI | CAYNNNNRTG | 1 cut(s) | 259 |
| SaqAI | TTAA | 2 cut(s) | 45, 243 |
| SatI | GCNGC | 1 cut(s) | 95 |
| SchI | GAGTC | 1 cut(s) | 63 |
| ScrFI | CCNGG | 2 cut(s) | 50, 82 |
| SetI | ASST | 4 cut(s) | 43, 96, 132, 194 |
| SfaNI | GCATC | 2 cut(s) | 21, 204 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 259 |
| SmlI | CTYRAG | 1 cut(s) | 193 |
| SmoI | CTYRAG | 1 cut(s) | 193 |
| Sse9I | AATT | 1 cut(s) | 226 |
| SsiI | CCGC | 2 cut(s) | 34, 61 |
| StyD4I | CCNGG | 2 cut(s) | 48, 80 |
| TaiI | ACGT | 1 cut(s) | 132 |
| TaqI | TCGA | 1 cut(s) | 178 |
| TasI | AATT | 1 cut(s) | 226 |
| Tru1I | TTAA | 2 cut(s) | 45, 243 |
| Tru9I | TTAA | 2 cut(s) | 45, 243 |
| TseI | GCWGC | 1 cut(s) | 94 |
| TspDTI | ATGAA | 1 cut(s) | 35 |