AT5G50340

Subunit ChlI of Mg-chelatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001026
Physical Location & Seq
Reverse (-)
20491393 .. 20496589
5197 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G50340.2

Sequence Viewer

Length: 1884 bp
ATGCTCTCGCAGAAGAAGACTGAGCGGCAAAGTCGTGTTTGTGAAATTGGCGGGTTTCGAACCTCGGAGCTCAGAACCCTAACCAGCAACAGTGGAGAAAGGTTTTTGAGTTTGTTGTTGATGTTGATCGCAGACATGAGATGCTTCAGATGCTTCTACACTCGAGCCCACTCTCTTCTCCTCCTCCGATCTTCCAATCCGGTCACGGTTCCTATATCTCCGGTCCGGTTCCGTCTTCGTCTCCGTCGTCTCTCATCATCTCCCGTCGCCTCCGTTTTCGACCTTCATTCCTCCAATCAGGACGCGAATTCGAACAGAGAAGACAAAGACACCAAAATTGGATTCTCCGATGATAAATCGAGAAAATCAGGTTCAAATCCTCCACGATGGAATCCAGGGGAAGTAGTTATGAACAAATCGAAGAAAGGGAAATCGAAAACTGTTTGGGTTTGTGAGAGCTGTGGTCACTCGGAAGGGCAATGGTGGGGGAGTTGTCGTGCTTGTCATAAGGTTGGGACTATGAAACGTTTTTCTGAAGGTTCTGAGTCAAGTGCTAGTGGTGGTGGTGGCAACGGTACTGGCTTGGGTTTTACGGAGGGTAAGGGTTTGTCTTGGTTACCTGAGCAAGCTACGGTTCAGCCACATCGATTGACTGATGTTATTCATGGCATTACTCAACAACAATGGAGAATTTCACTGCCTGGACTTTTTGGGAATGAAGTTGCTAGAGTACTTGGTGGTGGTCTTGCTCCAGGTTCTCTGATTTTGATTGGTGGTGATCCTGGTATTGGCAAGAGTACTTTGTTGTTGCAGATTGCATCTATAATTGCTGAGGGGAGTGAGTTGGCCGAACCAGCACCGGTTTTGTATATCTCTGGTGAAGAGAGTGTCGAGCAAATAGGAAGTCGGGCTGACAGGATGAGAATTCAAACTGAAGAGCTCTACCTGTTTTCGAGTTCGGATCTTCAGGACATTCTAAACAAAGCTCATCGACTATCTCCTCGAGCTCTCATAATTGACTCAATTCAAACCGTTTACCTAAAAGATGTGACTGGAAGTGCTGGTGGTCTCACACAGGTTAAAGAATGCACATCAACCCTGTTGCGTTTTGCTAAGAAATCTAATATCCCTGTTTTCTTGGTTGGACATGTTACGAAAGCGGGCGATATTGCAGGACCTCGTGTTTTGGAGCACATAGTAGATGTTGTCTTGTACTTGGAGGGTGAAGAGTCATCAACGTACCGCTTGCTTCGATCTGTGAAGAATCGGTTTGGGTCGACTGATGAACTTGGAGTTTTTGAAATGGCAAACTCAGGACTTGAAGTGGTCTCAAACCCAAGTGGCATATATCTTAGTCAACAGAACCCAGATTCAGATGTTTTAGCTGGATTAGCTGTAGCAGTTGTTATGGATGGATCTCGGAGTTTCCTAATTGAAGTTCAGGCATTGTGTTCCCCTGGCTCAACAGTTTCAAGGCATGTCAATGGTGTTCAAGCAAGCAGAGCTGACATGATAATTGCAGTTCTTATGAAGCAAGCTGGTCTTAGAATTCAGGAAAATGGGATTTTTCTGAACGTCGCTAACGGAATGGCTCTTTCTGAGACTGCTGGTGACCTTGCAATAGCAGCAGCTATTTGTAGCAGTTTCTTGGAGTTTCCAATTCCTCACGGTGTAGCATTCATTGGCGAGATTGGTCTTGGGGGAGAGGTTCGCACGGTACCAAGAATGGAGAAAAGGGTGAGTACAGTGGCAAAGCTTGGGTTTAACAAATGTGTAGTTCCTAAATCAGTGGAGGAATCACTTAAGGCGTTGAGTTTGAAAGAGATTGAGATAATCGGATGCAAGAATCTGAAAGAGTTAATCAATGCTGTGTTTAAGGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_25 ChlI

Protein Analysis

627

Amino Acids

67.64

Weight (kDa)

8.8

Isoelectric Point (pI)

46.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Zn_ribbon_LapB PF18073 109 - 135 4.4e-06 Rubredoxin metal binding domain
AAA_25 PF13481 204 - 349 2.7e-17 AAA domain
DnaB_C PF03796 207 - 313 4.2e-08 DnaB-like helicase C terminal domain
ChlI PF13541 466 - 555 1.9e-08 Subunit ChlI of Mg-chelatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1717
AccB1I GGYRCC 1 cut(s) 1717
AccBSI CCGCTC 1 cut(s) 25
AccI GTMKAC 1 cut(s) 1277
AccII CGCG 1 cut(s) 305
AciI CCGC 4 cut(s) 25, 51, 1160, 1243
AclI AACGTT 1 cut(s) 526
AclWI GGATC 3 cut(s) 773, 969, 1423
AcoI YGGCCR 1 cut(s) 846
AcsI RAATTY 4 cut(s) 307, 690, 924, 1548
AcuI CTGAAG 4 cut(s) 130, 555, 950, 954
AfaI GTAC 7 cut(s) 577, 732, 799, 1214, 1241, 1719, 1744
AfiI CCNNNNNNNGG 1 cut(s) 788
AflII CTTAAG 1 cut(s) 1802
AflIII ACRYGT 1 cut(s) 1147
AgeI ACCGGT 1 cut(s) 859
AgsI TTSAA 9 cut(s) 375, 929, 1028, 1301, 1322, 1436, 1473, 1493, 1819
AjnI CCWGG 5 cut(s) 394, 700, 751, 781, 1456
AjuI GAANNNNNNNTTGG 2 cut(s) 326, 358
Alw21I GWGCWC 4 cut(s) 72, 942, 1009, 1194
Alw26I GTCTC 5 cut(s) 245, 254, 1073, 1333, 1595
AlwI GGATC 3 cut(s) 773, 969, 1423
Ama87I CYCGRG 2 cut(s) 162, 1002
AoxI GGCC 1 cut(s) 846
ApeKI GCWGC 2 cut(s) 1625, 1628
ApoI RAATTY 4 cut(s) 307, 690, 924, 1548
ArsI GACNNNNNNTTYG 2 cut(s) 889, 921
AsiGI ACCGGT 1 cut(s) 859
Asp718I GGTACC 1 cut(s) 1717
AspS9I GGNCC 2 cut(s) 223, 1175
AsuHPI GGTGA 5 cut(s) 788, 890, 1235, 1622, 1750
AsuII TTCGAA 2 cut(s) 58, 311
AvaI CYCGRG 2 cut(s) 162, 1002
AvaII GGWCC 2 cut(s) 223, 1175
BanI GGYRCC 1 cut(s) 1717
BanII GRGCYC 4 cut(s) 72, 169, 942, 1009
BauI CACGAG 1 cut(s) 1179
BbsI GAAGAC 3 cut(s) 23, 227, 327
Bbv12I GWGCWC 4 cut(s) 72, 942, 1009, 1194
BbvCI CCTCAGC 1 cut(s) 831
BbvI GCAGC 2 cut(s) 1637, 1640
BccI CCATC 2 cut(s) 381, 1406
BciT130I CCWGG 5 cut(s) 396, 702, 753, 783, 1458
BcoDI GTCTC 5 cut(s) 245, 254, 1073, 1333, 1595
BfaI CTAG 2 cut(s) 555, 726
BfmI CTRYAG 1 cut(s) 1395
BfrI CTTAAG 1 cut(s) 1802
BisI GCNGC 3 cut(s) 26, 1626, 1629
BlsI GCNGC 3 cut(s) 27, 1627, 1630
BmcAI AGTACT 2 cut(s) 732, 799
Bme1390I CCNGG 5 cut(s) 396, 702, 753, 783, 1458
Bme18I GGWCC 2 cut(s) 223, 1175
BmeT110I CYCGRG 2 cut(s) 162, 1002
BmgT120I GGNCC 2 cut(s) 223, 1175
BmiI GGNNCC 3 cut(s) 210, 230, 1719
BmrFI CCNGG 5 cut(s) 396, 702, 753, 783, 1458
BmsI GCATC 4 cut(s) 131, 140, 827, 1829
BpiI GAAGAC 3 cut(s) 23, 227, 327
BpmI CTGGAG 1 cut(s) 735
Bpu10I CCTNAGC 2 cut(s) 621, 831
Bpu14I TTCGAA 2 cut(s) 58, 311
Bsa29I ATCGAT 1 cut(s) 646
BsaBI GATNNNNATC 1 cut(s) 125
BsaI GGTCTC 2 cut(s) 1073, 1333
BsaJI CCNNGG 3 cut(s) 63, 395, 1456
BsaWI WCCGGW 4 cut(s) 199, 220, 225, 859
BsaXI ACNNNNNCTCC 2 cut(s) 481, 511
Bsc4I CCNNNNNNNGG 1 cut(s) 788
Bse118I RCCGGY 1 cut(s) 859
Bse1I ACTGG 2 cut(s) 583, 1057
Bse3DI GCAATG 1 cut(s) 485
Bse8I GATNNNNATC 1 cut(s) 125
BseBI CCWGG 5 cut(s) 396, 702, 753, 783, 1458
BseCI ATCGAT 1 cut(s) 646
BseDI CCNNGG 3 cut(s) 63, 395, 1456
BseGI GGATG 3 cut(s) 924, 1417, 1844
BseJI GATNNNNATC 1 cut(s) 125
BseLI CCNNNNNNNGG 1 cut(s) 788
BseMI GCAATG 1 cut(s) 485
BseMII CTCAG 7 cut(s) 12, 85, 534, 612, 822, 1326, 1590
BseNI ACTGG 2 cut(s) 583, 1057
BseRI GAGGAG 3 cut(s) 170, 173, 990
BseXI GCAGC 2 cut(s) 1637, 1640
Bsh1236I CGCG 1 cut(s) 305
BshFI GGCC 1 cut(s) 848
BshNI GGYRCC 1 cut(s) 1717
BshTI ACCGGT 1 cut(s) 859
BshVI ATCGAT 1 cut(s) 646
BsiHKAI GWGCWC 4 cut(s) 72, 942, 1009, 1194
BsiHKCI CYCGRG 2 cut(s) 162, 1002
BsiSI CCGG 4 cut(s) 200, 221, 226, 860
BslFI GGGAC 1 cut(s) 529
BslI CCNNNNNNNGG 1 cut(s) 788
BsmAI GTCTC 5 cut(s) 245, 254, 1073, 1333, 1595
BsmBI CGTCTC 2 cut(s) 245, 254
BsmFI GGGAC 1 cut(s) 529
BsmI GAATGC 2 cut(s) 1091, 1676
BsnI GGCC 1 cut(s) 848
Bso31I GGTCTC 2 cut(s) 1073, 1333
BsoBI CYCGRG 2 cut(s) 162, 1002
Bsp119I TTCGAA 2 cut(s) 58, 311
Bsp1286I GDGCHC 5 cut(s) 72, 169, 942, 1009, 1194
Bsp143I GATC 6 cut(s) 126, 188, 778, 961, 1253, 1415
BspACI CCGC 4 cut(s) 25, 51, 1160, 1243
BspANI GGCC 1 cut(s) 848
BspCNI CTCAG 7 cut(s) 13, 84, 535, 613, 823, 1325, 1591
BspDI ATCGAT 1 cut(s) 646
BspFNI CGCG 1 cut(s) 305
BspLI GGNNCC 3 cut(s) 210, 230, 1719
BspPI GGATC 3 cut(s) 773, 969, 1423
BspQI GCTCTTC 1 cut(s) 930
BspT104I TTCGAA 2 cut(s) 58, 311
BspT107I GGYRCC 1 cut(s) 1717
BspTI CTTAAG 1 cut(s) 1802
BspTNI GGTCTC 2 cut(s) 1073, 1333
BsrBI CCGCTC 1 cut(s) 25
BsrDI GCAATG 1 cut(s) 485
BsrFI RCCGGY 1 cut(s) 859
BsrI ACTGG 2 cut(s) 583, 1057
BssAI RCCGGY 1 cut(s) 859
BssECI CCNNGG 3 cut(s) 63, 395, 1456
BssMI GATC 6 cut(s) 126, 188, 778, 961, 1253, 1415
BssSI CACGAG 1 cut(s) 1179
Bst2BI CACGAG 1 cut(s) 1179
Bst2UI CCWGG 5 cut(s) 396, 702, 753, 783, 1458
Bst6I CTCTTC 4 cut(s) 180, 876, 930, 1221
BstAFI CTTAAG 1 cut(s) 1802
BstBI TTCGAA 2 cut(s) 58, 311
BstC8I GCNNGC 5 cut(s) 627, 1162, 1247, 1498, 1536
BstEII GGTNACC 2 cut(s) 615, 1610
BstF5I GGATG 3 cut(s) 924, 1417, 1844
BstFNI CGCG 1 cut(s) 305
BstKTI GATC 6 cut(s) 129, 191, 781, 964, 1256, 1418
BstMAI GTCTC 5 cut(s) 245, 254, 1073, 1333, 1595
BstMBI GATC 6 cut(s) 126, 188, 778, 961, 1253, 1415
BstMWI GCNNNNNNNGC 5 cut(s) 150, 854, 1391, 1502, 1625
BstNI CCWGG 5 cut(s) 396, 702, 753, 783, 1458
BstNSI RCATGY 2 cut(s) 1151, 1481
BstPI GGTNACC 2 cut(s) 615, 1610
BstSCI CCNGG 5 cut(s) 394, 700, 751, 781, 1456
BstSFI CTRYAG 1 cut(s) 1395
BstUI CGCG 1 cut(s) 305
BstV1I GCAGC 2 cut(s) 1637, 1640
BstV2I GAAGAC 3 cut(s) 23, 227, 327
BstX2I RGATCY 2 cut(s) 961, 1415
BstYI RGATCY 2 cut(s) 961, 1415
Bsu15I ATCGAT 1 cut(s) 646
BsuRI GGCC 1 cut(s) 848
BsuTUI ATCGAT 1 cut(s) 646
BtsCI GGATG 3 cut(s) 924, 1417, 1844
BtsI GCAGTG 1 cut(s) 695
BtsIMutI CAGTG 4 cut(s) 97, 695, 1752, 1794
Cac8I GCNNGC 5 cut(s) 627, 1162, 1247, 1498, 1536
Cfr10I RCCGGY 1 cut(s) 859
Cfr13I GGNCC 2 cut(s) 223, 1175
ClaI ATCGAT 1 cut(s) 646
CpoI CGGWCCG 1 cut(s) 223
CseI GACGC 1 cut(s) 311
Csp6I GTAC 7 cut(s) 576, 731, 798, 1213, 1240, 1718, 1743
CspAI ACCGGT 1 cut(s) 859
CspCI CAANNNNNGTGG 2 cut(s) 630, 665
CspI CGGWCCG 1 cut(s) 223
CviAII CATG 5 cut(s) 136, 665, 1148, 1478, 1510
CviQI GTAC 7 cut(s) 576, 731, 798, 1213, 1240, 1718, 1743
DpnI GATC 6 cut(s) 128, 190, 780, 963, 1255, 1417
DpnII GATC 6 cut(s) 126, 188, 778, 961, 1253, 1415
EaeI YGGCCR 1 cut(s) 846
Eam1104I CTCTTC 4 cut(s) 180, 876, 930, 1221
EarI CTCTTC 4 cut(s) 180, 876, 930, 1221
Ecl136II GAGCTC 3 cut(s) 70, 940, 1007
Eco24I GRGCYC 4 cut(s) 72, 169, 942, 1009
Eco31I GGTCTC 2 cut(s) 1073, 1333
Eco47I GGWCC 2 cut(s) 223, 1175
Eco53kI GAGCTC 3 cut(s) 70, 940, 1007
Eco57I CTGAAG 4 cut(s) 130, 555, 950, 954
Eco88I CYCGRG 2 cut(s) 162, 1002
Eco91I GGTNACC 2 cut(s) 615, 1610
EcoICRI GAGCTC 3 cut(s) 70, 940, 1007
EcoO109I RGGNCCY 1 cut(s) 1175
EcoO65I GGTNACC 2 cut(s) 615, 1610
EcoRI GAATTC 3 cut(s) 307, 924, 1548
EcoRII CCWGG 5 cut(s) 394, 700, 751, 781, 1456
EcoT38I GRGCYC 4 cut(s) 72, 169, 942, 1009
Esp3I CGTCTC 2 cut(s) 245, 254
FaeI CATG 5 cut(s) 139, 668, 1151, 1481, 1513
FalI AAGNNNNNCTT 2 cut(s) 1527, 1559
FaqI GGGAC 1 cut(s) 529
FatI CATG 5 cut(s) 135, 664, 1147, 1477, 1509
FauI CCCGC 2 cut(s) 44, 1153
FblI GTMKAC 1 cut(s) 1277
Fnu4HI GCNGC 3 cut(s) 26, 1626, 1629
FokI GGATG 3 cut(s) 931, 1424, 1851
FriOI GRGCYC 4 cut(s) 72, 169, 942, 1009
Fsp4HI GCNGC 3 cut(s) 26, 1626, 1629
FspBI CTAG 2 cut(s) 555, 726
GluI GCNGC 3 cut(s) 26, 1626, 1629
GsuI CTGGAG 1 cut(s) 735
HaeIII GGCC 1 cut(s) 848
HapII CCGG 4 cut(s) 200, 221, 226, 860
HgaI GACGC 1 cut(s) 311
Hin1II CATG 5 cut(s) 139, 668, 1151, 1481, 1513
HincII GTYRAC 2 cut(s) 1278, 1358
HindII GTYRAC 2 cut(s) 1278, 1358
HindIII AAGCTT 1 cut(s) 1754
HinfI GANTC 9 cut(s) 342, 391, 545, 1019, 1229, 1264, 1370, 1796, 1846
HpaII CCGG 4 cut(s) 200, 221, 226, 860
HphI GGTGA 5 cut(s) 788, 890, 1235, 1622, 1750
Hpy166II GTNNAC 3 cut(s) 1036, 1278, 1358
Hpy188III TCNNGA 5 cut(s) 299, 360, 968, 1314, 1553
Hpy8I GTNNAC 3 cut(s) 1036, 1278, 1358
Hpy99I CGWCG 3 cut(s) 249, 269, 1580
HpyAV CCTTC 3 cut(s) 293, 467, 530
HpyCH4IV ACGT 3 cut(s) 526, 1238, 1575
HpyCH4V TGCA 7 cut(s) 811, 818, 1089, 1172, 1520, 1619, 1842
HpyF10VI GCNNNNNNNGC 5 cut(s) 150, 854, 1391, 1502, 1625
HpySE526I ACGT 3 cut(s) 526, 1238, 1575
Hsp92II CATG 5 cut(s) 139, 668, 1151, 1481, 1513
KpnI GGTACC 1 cut(s) 1721
Kzo9I GATC 6 cut(s) 126, 188, 778, 961, 1253, 1415
LguI GCTCTTC 1 cut(s) 930
LmnI GCTCC 3 cut(s) 67, 754, 1189
Lsp1109I GCAGC 2 cut(s) 1637, 1640
LweI GCATC 4 cut(s) 131, 140, 827, 1829
MaeI CTAG 2 cut(s) 555, 726
MaeII ACGT 3 cut(s) 526, 1238, 1575
MaeIII GTNAC 6 cut(s) 202, 464, 615, 1048, 1150, 1610
MalI GATC 6 cut(s) 128, 190, 780, 963, 1255, 1417
MbiI CCGCTC 1 cut(s) 25
MboI GATC 6 cut(s) 126, 188, 778, 961, 1253, 1415
MflI RGATCY 2 cut(s) 961, 1415
MhlI GDGCHC 5 cut(s) 72, 169, 942, 1009, 1194
MlyI GAGTC 3 cut(s) 554, 1013, 1238
MmeI TCCRAC 1 cut(s) 1123
MseI TTAA 5 cut(s) 1080, 1764, 1803, 1859, 1875
MslI CAYNNNNRTG 1 cut(s) 1482
MspCI CTTAAG 1 cut(s) 1802
MspI CCGG 4 cut(s) 200, 221, 226, 860
MspR9I CCNGG 5 cut(s) 396, 702, 753, 783, 1458
Mva1269I GAATGC 2 cut(s) 1091, 1676
MvaI CCWGG 5 cut(s) 396, 702, 753, 783, 1458
MvnI CGCG 1 cut(s) 305
MwoI GCNNNNNNNGC 5 cut(s) 150, 854, 1391, 1502, 1625
NdeII GATC 6 cut(s) 126, 188, 778, 961, 1253, 1415
NlaIII CATG 5 cut(s) 139, 668, 1151, 1481, 1513
NlaIV GGNNCC 3 cut(s) 210, 230, 1719
NmuCI GTSAC 4 cut(s) 202, 464, 1048, 1610
NspI RCATGY 2 cut(s) 1151, 1481
NspV TTCGAA 2 cut(s) 58, 311
PaeR7I CTCGAG 2 cut(s) 162, 1002
PciI ACATGT 1 cut(s) 1147
PciSI GCTCTTC 1 cut(s) 930
PcsI WCGNNNNNNNCGW 1 cut(s) 244
PctI GAATGC 2 cut(s) 1091, 1676
PfeI GAWTC 6 cut(s) 342, 391, 1264, 1370, 1796, 1846
PinAI ACCGGT 1 cut(s) 859
PkrI GCNGC 3 cut(s) 27, 1627, 1630
PleI GAGTC 3 cut(s) 553, 1013, 1237
PpsI GAGTC 3 cut(s) 553, 1013, 1237
PpuMI RGGWCCY 1 cut(s) 1175
PscI ACATGT 1 cut(s) 1147
Psp124BI GAGCTC 3 cut(s) 72, 942, 1009
Psp1406I AACGTT 1 cut(s) 526
Psp5II RGGWCCY 1 cut(s) 1175
Psp6I CCWGG 5 cut(s) 394, 700, 751, 781, 1456
PspEI GGTNACC 2 cut(s) 615, 1610
PspGI CCWGG 5 cut(s) 394, 700, 751, 781, 1456
PspN4I GGNNCC 3 cut(s) 210, 230, 1719
PspPI GGNCC 2 cut(s) 223, 1175
PspPPI RGGWCCY 1 cut(s) 1175
PspXI VCTCGAGB 2 cut(s) 162, 1002
PsuI RGATCY 2 cut(s) 961, 1415
RsaI GTAC 7 cut(s) 577, 732, 799, 1214, 1241, 1719, 1744
RsaNI GTAC 7 cut(s) 576, 731, 798, 1213, 1240, 1718, 1743
RseI CAYNNNNRTG 1 cut(s) 1482
Rsr2I CGGWCCG 1 cut(s) 223
RsrII CGGWCCG 1 cut(s) 223
SacI GAGCTC 3 cut(s) 72, 942, 1009
SalI GTCGAC 1 cut(s) 1276
SapI GCTCTTC 1 cut(s) 930
SaqAI TTAA 5 cut(s) 1080, 1764, 1803, 1859, 1875
SatI GCNGC 3 cut(s) 26, 1626, 1629
Sau3AI GATC 6 cut(s) 126, 188, 778, 961, 1253, 1415
Sau96I GGNCC 2 cut(s) 223, 1175
ScaI AGTACT 2 cut(s) 732, 799
SchI GAGTC 3 cut(s) 554, 1013, 1238
ScrFI CCNGG 5 cut(s) 396, 702, 753, 783, 1458
SduI GDGCHC 5 cut(s) 72, 169, 942, 1009, 1194
SfaNI GCATC 4 cut(s) 131, 140, 827, 1829
SfcI CTRYAG 1 cut(s) 1395
Sfr274I CTCGAG 2 cut(s) 162, 1002
SfuI TTCGAA 2 cut(s) 58, 311
SinI GGWCC 2 cut(s) 223, 1175
SlaI CTCGAG 2 cut(s) 162, 1002
SmiMI CAYNNNNRTG 1 cut(s) 1482
SmlI CTYRAG 3 cut(s) 162, 1002, 1802
SmoI CTYRAG 3 cut(s) 162, 1002, 1802
SsiI CCGC 4 cut(s) 25, 51, 1160, 1243
SspMI CTAG 2 cut(s) 555, 726
SstI GAGCTC 3 cut(s) 72, 942, 1009
StyD4I CCNGG 5 cut(s) 394, 700, 751, 781, 1456
TaiI ACGT 3 cut(s) 529, 1241, 1578
TatI WGTACW 4 cut(s) 730, 797, 1212, 1742
TauI GCSGC 1 cut(s) 28
TfiI GAWTC 6 cut(s) 342, 391, 1264, 1370, 1796, 1846
Tru1I TTAA 5 cut(s) 1080, 1764, 1803, 1859, 1875
Tru9I TTAA 5 cut(s) 1080, 1764, 1803, 1859, 1875
TscAI CASTG 4 cut(s) 97, 702, 1752, 1794
TseFI GTSAC 4 cut(s) 202, 464, 1048, 1610
TseI GCWGC 2 cut(s) 1625, 1628
Tsp45I GTSAC 4 cut(s) 202, 464, 1048, 1610
TspDTI ATGAA 8 cut(s) 275, 425, 536, 653, 732, 1299, 1544, 1669
TspGWI ACGGA 5 cut(s) 221, 233, 262, 608, 1599
TspRI CASTG 4 cut(s) 97, 702, 1752, 1794
Vha464I CTTAAG 1 cut(s) 1802
VpaK11BI GGWCC 2 cut(s) 223, 1175
XapI RAATTY 4 cut(s) 307, 690, 924, 1548
XceI RCATGY 2 cut(s) 1151, 1481
XhoI CTCGAG 2 cut(s) 162, 1002
XmiI GTMKAC 1 cut(s) 1277
XspI CTAG 2 cut(s) 555, 726
ZrmI AGTACT 2 cut(s) 732, 799
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.