Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| Topoisom_bac | PF01131 | 68 - 193 | 3.4e-30 | DNA topoisomerase |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 194 |
| Acc36I | ACCTGC | 1 cut(s) | 194 |
| AccB1I | GGYRCC | 2 cut(s) | 4, 76 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1109 |
| AccII | CGCG | 1 cut(s) | 143 |
| AciI | CCGC | 6 cut(s) | 26, 143, 676, 1054, 1146, 1167 |
| AclWI | GGATC | 3 cut(s) | 644, 859, 1338 |
| AcoI | YGGCCR | 1 cut(s) | 144 |
| AcsI | RAATTY | 4 cut(s) | 347, 801, 1000, 1098 |
| AcyI | GRCGYC | 1 cut(s) | 77 |
| AfaI | GTAC | 7 cut(s) | 582, 937, 1153, 1158, 1192, 1343, 1351 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 502, 1017, 1109, 1166 |
| AflIII | ACRYGT | 1 cut(s) | 388 |
| AgsI | TTSAA | 5 cut(s) | 91, 185, 354, 1060, 1421 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 70 |
| AjnI | CCWGG | 1 cut(s) | 1453 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 339, 371 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1449, 1481 |
| AluBI | AGCT | 7 cut(s) | 544, 587, 818, 1223, 1237, 1304, 1400 |
| AluI | AGCT | 7 cut(s) | 544, 587, 818, 1223, 1237, 1304, 1400 |
| Alw26I | GTCTC | 3 cut(s) | 951, 1270, 1389 |
| AlwI | GGATC | 3 cut(s) | 644, 859, 1338 |
| Ama87I | CYCGRG | 1 cut(s) | 10 |
| AoxI | GGCC | 2 cut(s) | 54, 144 |
| ApeKI | GCWGC | 4 cut(s) | 691, 742, 818, 1211 |
| ApoI | RAATTY | 4 cut(s) | 347, 801, 1000, 1098 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 801 |
| AspLEI | GCGC | 2 cut(s) | 79, 908 |
| AspS9I | GGNCC | 2 cut(s) | 493, 848 |
| AsuHPI | GGTGA | 7 cut(s) | 89, 218, 409, 507, 1027, 1181, 1283 |
| AvaI | CYCGRG | 1 cut(s) | 10 |
| AvaII | GGWCC | 2 cut(s) | 493, 848 |
| AxyI | CCTNAGG | 1 cut(s) | 613 |
| BanI | GGYRCC | 2 cut(s) | 4, 76 |
| BbvI | GCAGC | 4 cut(s) | 703, 754, 805, 1198 |
| BceAI | ACGGC | 2 cut(s) | 64, 131 |
| BciT130I | CCWGG | 1 cut(s) | 1455 |
| BclI | TGATCA | 1 cut(s) | 208 |
| BcoDI | GTCTC | 3 cut(s) | 951, 1270, 1389 |
| BfmI | CTRYAG | 1 cut(s) | 819 |
| BfoI | RGCGCY | 1 cut(s) | 80 |
| BfuAI | ACCTGC | 1 cut(s) | 194 |
| BglII | AGATCT | 2 cut(s) | 187, 655 |
| BisI | GCNGC | 5 cut(s) | 144, 692, 743, 819, 1212 |
| BlsI | GCNGC | 5 cut(s) | 145, 693, 744, 820, 1213 |
| BmcAI | AGTACT | 2 cut(s) | 937, 1351 |
| Bme1390I | CCNGG | 1 cut(s) | 1455 |
| Bme18I | GGWCC | 2 cut(s) | 493, 848 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 70 |
| BmeT110I | CYCGRG | 1 cut(s) | 10 |
| BmgT120I | GGNCC | 2 cut(s) | 493, 848 |
| BmiI | GGNNCC | 7 cut(s) | 6, 78, 603, 849, 850, 1114, 1392 |
| BmrFI | CCNGG | 1 cut(s) | 1455 |
| BmrI | ACTGGG | 2 cut(s) | 30, 1081 |
| BmsI | GCATC | 3 cut(s) | 190, 253, 1256 |
| BmuI | ACTGGG | 2 cut(s) | 30, 1081 |
| BpmI | CTGGAG | 2 cut(s) | 504, 1476 |
| Bpu10I | CCTNAGC | 2 cut(s) | 113, 711 |
| BpuEI | CTTGAG | 2 cut(s) | 1330, 1380 |
| Bsa29I | ATCGAT | 1 cut(s) | 876 |
| BsaBI | GATNNNNATC | 1 cut(s) | 654 |
| BsaHI | GRCGYC | 1 cut(s) | 77 |
| BsaI | GGTCTC | 1 cut(s) | 1389 |
| BsaJI | CCNNGG | 2 cut(s) | 422, 1011 |
| BsaWI | WCCGGW | 1 cut(s) | 1268 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 298, 328, 502, 532, 1449, 1479 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 502, 1017, 1109, 1166 |
| Bse118I | RCCGGY | 1 cut(s) | 56 |
| Bse1I | ACTGG | 4 cut(s) | 36, 987, 1076, 1160 |
| Bse21I | CCTNAGG | 1 cut(s) | 613 |
| Bse3DI | GCAATG | 1 cut(s) | 1103 |
| Bse8I | GATNNNNATC | 1 cut(s) | 654 |
| BseBI | CCWGG | 1 cut(s) | 1455 |
| BseCI | ATCGAT | 1 cut(s) | 876 |
| BseDI | CCNNGG | 2 cut(s) | 422, 1011 |
| BseGI | GGATG | 2 cut(s) | 211, 1247 |
| BseJI | GATNNNNATC | 1 cut(s) | 654 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 502, 1017, 1109, 1166 |
| BseMI | GCAATG | 1 cut(s) | 1103 |
| BseMII | CTCAG | 3 cut(s) | 234, 871, 962 |
| BseNI | ACTGG | 4 cut(s) | 36, 987, 1076, 1160 |
| BseRI | GAGGAG | 1 cut(s) | 1383 |
| BseX3I | CGGCCG | 1 cut(s) | 144 |
| BseXI | GCAGC | 4 cut(s) | 703, 754, 805, 1198 |
| BseYI | CCCAGC | 2 cut(s) | 253, 1237 |
| BsgI | GTGCAG | 2 cut(s) | 761, 1197 |
| Bsh1236I | CGCG | 1 cut(s) | 143 |
| Bsh1285I | CGRYCG | 2 cut(s) | 26, 147 |
| BshFI | GGCC | 2 cut(s) | 56, 146 |
| BshNI | GGYRCC | 2 cut(s) | 4, 76 |
| BshVI | ATCGAT | 1 cut(s) | 876 |
| BsiEI | CGRYCG | 2 cut(s) | 26, 147 |
| BsiHKCI | CYCGRG | 1 cut(s) | 10 |
| BsiSI | CCGG | 2 cut(s) | 57, 1269 |
| BslFI | GGGAC | 3 cut(s) | 24, 319, 861 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 502, 1017, 1109, 1166 |
| BsmAI | GTCTC | 3 cut(s) | 951, 1270, 1389 |
| BsmFI | GGGAC | 3 cut(s) | 24, 319, 861 |
| BsmI | GAATGC | 1 cut(s) | 979 |
| BsnI | GGCC | 2 cut(s) | 56, 146 |
| Bso31I | GGTCTC | 1 cut(s) | 1389 |
| BsoBI | CYCGRG | 1 cut(s) | 10 |
| Bsp19I | CCATGG | 2 cut(s) | 422, 1011 |
| BspACI | CCGC | 6 cut(s) | 26, 143, 676, 1054, 1146, 1167 |
| BspANI | GGCC | 2 cut(s) | 56, 146 |
| BspCNI | CTCAG | 3 cut(s) | 235, 870, 961 |
| BspDI | ATCGAT | 1 cut(s) | 876 |
| BspFNI | CGCG | 1 cut(s) | 143 |
| BspHI | TCATGA | 1 cut(s) | 499 |
| BspLI | GGNNCC | 7 cut(s) | 6, 78, 603, 849, 850, 1114, 1392 |
| BspMAI | CTGCAG | 1 cut(s) | 823 |
| BspMI | ACCTGC | 1 cut(s) | 194 |
| BspPI | GGATC | 3 cut(s) | 644, 859, 1338 |
| BspT107I | GGYRCC | 2 cut(s) | 4, 76 |
| BspTNI | GGTCTC | 1 cut(s) | 1389 |
| BsrDI | GCAATG | 1 cut(s) | 1103 |
| BsrFI | RCCGGY | 1 cut(s) | 56 |
| BsrI | ACTGG | 4 cut(s) | 36, 987, 1076, 1160 |
| BssAI | RCCGGY | 1 cut(s) | 56 |
| BssECI | CCNNGG | 2 cut(s) | 422, 1011 |
| BssNI | GRCGYC | 1 cut(s) | 77 |
| BssT1I | CCWWGG | 2 cut(s) | 422, 1011 |
| Bst2UI | CCWGG | 1 cut(s) | 1455 |
| Bst4CI | ACNGT | 8 cut(s) | 109, 310, 580, 759, 776, 1138, 1156, 1426 |
| Bst6I | CTCTTC | 1 cut(s) | 1269 |
| BstACI | GRCGYC | 1 cut(s) | 77 |
| BstC8I | GCNNGC | 4 cut(s) | 58, 589, 816, 1183 |
| BstDEI | CTNAG | 9 cut(s) | 113, 243, 530, 613, 711, 857, 921, 948, 1175 |
| BstDSI | CCRYGG | 2 cut(s) | 422, 1011 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 500 |
| BstF5I | GGATG | 2 cut(s) | 211, 1247 |
| BstFNI | CGCG | 1 cut(s) | 143 |
| BstH2I | RGCGCY | 1 cut(s) | 80 |
| BstHHI | GCGC | 2 cut(s) | 79, 908 |
| BstMAI | GTCTC | 3 cut(s) | 951, 1270, 1389 |
| BstMCI | CGRYCG | 2 cut(s) | 26, 147 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 167, 176, 550, 1220, 1397, 1406 |
| BstNI | CCWGG | 1 cut(s) | 1455 |
| BstNSI | RCATGY | 1 cut(s) | 749 |
| BstSCI | CCNGG | 1 cut(s) | 1453 |
| BstSFI | CTRYAG | 1 cut(s) | 819 |
| BstUI | CGCG | 1 cut(s) | 143 |
| BstV1I | GCAGC | 4 cut(s) | 703, 754, 805, 1198 |
| BstX2I | RGATCY | 4 cut(s) | 187, 655, 864, 1330 |
| BstYI | RGATCY | 4 cut(s) | 187, 655, 864, 1330 |
| BstZI | CGGCCG | 1 cut(s) | 144 |
| Bsu15I | ATCGAT | 1 cut(s) | 876 |
| Bsu36I | CCTNAGG | 1 cut(s) | 613 |
| BsuRI | GGCC | 2 cut(s) | 56, 146 |
| BsuTUI | ATCGAT | 1 cut(s) | 876 |
| BtgI | CCRYGG | 2 cut(s) | 422, 1011 |
| BtgZI | GCGATG | 1 cut(s) | 942 |
| BtsCI | GGATG | 2 cut(s) | 211, 1247 |
| BtsI | GCAGTG | 1 cut(s) | 1468 |
| BtsIMutI | CAGTG | 2 cut(s) | 114, 1468 |
| BveI | ACCTGC | 1 cut(s) | 194 |
| Cac8I | GCNNGC | 4 cut(s) | 58, 589, 816, 1183 |
| CciI | TCATGA | 1 cut(s) | 499 |
| CfoI | GCGC | 2 cut(s) | 79, 908 |
| Cfr10I | RCCGGY | 1 cut(s) | 56 |
| Cfr13I | GGNCC | 2 cut(s) | 493, 848 |
| ClaI | ATCGAT | 1 cut(s) | 876 |
| Csp6I | GTAC | 7 cut(s) | 581, 936, 1152, 1157, 1191, 1342, 1350 |
| CviAII | CATG | 6 cut(s) | 423, 500, 746, 1012, 1109, 1232 |
| CviQI | GTAC | 7 cut(s) | 581, 936, 1152, 1157, 1191, 1342, 1350 |
| DdeI | CTNAG | 9 cut(s) | 113, 243, 530, 613, 711, 857, 921, 948, 1175 |
| DinI | GGCGCC | 1 cut(s) | 78 |
| DraI | TTTAAA | 2 cut(s) | 1260, 1434 |
| DriI | GACNNNNNGTC | 1 cut(s) | 70 |
| EaeI | YGGCCR | 1 cut(s) | 144 |
| EagI | CGGCCG | 1 cut(s) | 144 |
| Eam1104I | CTCTTC | 1 cut(s) | 1269 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 70 |
| EarI | CTCTTC | 1 cut(s) | 1269 |
| EclXI | CGGCCG | 1 cut(s) | 144 |
| Eco130I | CCWWGG | 2 cut(s) | 422, 1011 |
| Eco31I | GGTCTC | 1 cut(s) | 1389 |
| Eco32I | GATATC | 1 cut(s) | 673 |
| Eco47I | GGWCC | 2 cut(s) | 493, 848 |
| Eco52I | CGGCCG | 1 cut(s) | 144 |
| Eco81I | CCTNAGG | 1 cut(s) | 613 |
| Eco88I | CYCGRG | 1 cut(s) | 10 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 500 |
| EcoO109I | RGGNCCY | 1 cut(s) | 848 |
| EcoRI | GAATTC | 1 cut(s) | 1098 |
| EcoRII | CCWGG | 1 cut(s) | 1453 |
| EcoRV | GATATC | 1 cut(s) | 673 |
| EcoT14I | CCWWGG | 2 cut(s) | 422, 1011 |
| EgeI | GGCGCC | 1 cut(s) | 78 |
| EheI | GGCGCC | 1 cut(s) | 78 |
| ErhI | CCWWGG | 2 cut(s) | 422, 1011 |
| FaeI | CATG | 6 cut(s) | 426, 503, 749, 1015, 1112, 1235 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 177, 209 |
| FaqI | GGGAC | 3 cut(s) | 24, 319, 861 |
| FatI | CATG | 6 cut(s) | 422, 499, 745, 1011, 1108, 1231 |
| FauNDI | CATATG | 1 cut(s) | 1502 |
| FbaI | TGATCA | 1 cut(s) | 208 |
| Fnu4HI | GCNGC | 5 cut(s) | 144, 692, 743, 819, 1212 |
| FokI | GGATG | 2 cut(s) | 198, 1234 |
| Fsp4HI | GCNGC | 5 cut(s) | 144, 692, 743, 819, 1212 |
| GlaI | GCGC | 2 cut(s) | 78, 907 |
| GluI | GCNGC | 5 cut(s) | 144, 692, 743, 819, 1212 |
| GsaI | CCCAGC | 2 cut(s) | 257, 1241 |
| GsuI | CTGGAG | 2 cut(s) | 504, 1476 |
| HaeII | RGCGCY | 1 cut(s) | 80 |
| HaeIII | GGCC | 2 cut(s) | 56, 146 |
| HapII | CCGG | 2 cut(s) | 57, 1269 |
| HhaI | GCGC | 2 cut(s) | 79, 908 |
| Hin1I | GRCGYC | 1 cut(s) | 77 |
| Hin1II | CATG | 6 cut(s) | 426, 503, 749, 1015, 1112, 1235 |
| Hin6I | GCGC | 2 cut(s) | 77, 906 |
| HinP1I | GCGC | 2 cut(s) | 77, 906 |
| HinfI | GANTC | 4 cut(s) | 13, 94, 915, 1412 |
| HpaII | CCGG | 2 cut(s) | 57, 1269 |
| HphI | GGTGA | 7 cut(s) | 89, 218, 409, 507, 1027, 1181, 1283 |
| Hpy166II | GTNNAC | 4 cut(s) | 293, 493, 1069, 1193 |
| Hpy188I | TCNGA | 4 cut(s) | 23, 764, 901, 951 |
| Hpy188III | TCNNGA | 6 cut(s) | 317, 500, 521, 653, 846, 1204 |
| Hpy8I | GTNNAC | 4 cut(s) | 293, 493, 1069, 1193 |
| HpyAV | CCTTC | 3 cut(s) | 394, 506, 1033 |
| HpyCH4III | ACNGT | 8 cut(s) | 109, 310, 580, 759, 776, 1138, 1156, 1426 |
| HpyCH4IV | ACGT | 1 cut(s) | 388 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 167, 176, 550, 1220, 1397, 1406 |
| HpyF3I | CTNAG | 9 cut(s) | 113, 243, 530, 613, 711, 857, 921, 948, 1175 |
| HpySE526I | ACGT | 1 cut(s) | 388 |
| Hsp92I | GRCGYC | 1 cut(s) | 77 |
| Hsp92II | CATG | 6 cut(s) | 426, 503, 749, 1015, 1112, 1235 |
| HspAI | GCGC | 2 cut(s) | 77, 906 |
| KasI | GGCGCC | 1 cut(s) | 76 |
| KflI | GGGWCCC | 1 cut(s) | 848 |
| KroI | GCCGGC | 1 cut(s) | 56 |
| KroNI | GCCGGC | 1 cut(s) | 58 |
| Ksp22I | TGATCA | 1 cut(s) | 208 |
| LmnI | GCTCC | 2 cut(s) | 1296, 1396 |
| Lsp1109I | GCAGC | 4 cut(s) | 703, 754, 805, 1198 |
| LweI | GCATC | 3 cut(s) | 190, 253, 1256 |
| MaeII | ACGT | 1 cut(s) | 388 |
| MaeIII | GTNAC | 2 cut(s) | 220, 1487 |
| MfeI | CAATTG | 1 cut(s) | 354 |
| MflI | RGATCY | 4 cut(s) | 187, 655, 864, 1330 |
| Mly113I | GGCGCC | 1 cut(s) | 77 |
| MlyI | GAGTC | 2 cut(s) | 22, 909 |
| MmeI | TCCRAC | 7 cut(s) | 46, 130, 629, 774, 787, 1064, 1453 |
| MroNI | GCCGGC | 1 cut(s) | 56 |
| MroXI | GAANNNNTTC | 1 cut(s) | 801 |
| MseI | TTAA | 5 cut(s) | 51, 1044, 1259, 1320, 1433 |
| MslI | CAYNNNNRTG | 1 cut(s) | 732 |
| MspA1I | CMGCKG | 1 cut(s) | 1223 |
| MspI | CCGG | 2 cut(s) | 57, 1269 |
| MspR9I | CCNGG | 1 cut(s) | 1455 |
| MunI | CAATTG | 1 cut(s) | 354 |
| Mva1269I | GAATGC | 1 cut(s) | 979 |
| MvaI | CCWGG | 1 cut(s) | 1455 |
| MvnI | CGCG | 1 cut(s) | 143 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 167, 176, 550, 1220, 1397, 1406 |
| NaeI | GCCGGC | 1 cut(s) | 58 |
| NarI | GGCGCC | 1 cut(s) | 77 |
| NcoI | CCATGG | 2 cut(s) | 422, 1011 |
| NdeI | CATATG | 1 cut(s) | 1502 |
| NgoMIV | GCCGGC | 1 cut(s) | 56 |
| NlaIII | CATG | 6 cut(s) | 426, 503, 749, 1015, 1112, 1235 |
| NlaIV | GGNNCC | 7 cut(s) | 6, 78, 603, 849, 850, 1114, 1392 |
| NspI | RCATGY | 1 cut(s) | 749 |
| PagI | TCATGA | 1 cut(s) | 499 |
| PaqCI | CACCTGC | 1 cut(s) | 194 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 811 |
| PctI | GAATGC | 1 cut(s) | 979 |
| PdiI | GCCGGC | 1 cut(s) | 58 |
| PdmI | GAANNNNTTC | 1 cut(s) | 801 |
| PfeI | GAWTC | 2 cut(s) | 94, 1412 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1109 |
| PfoI | TCCNGGA | 1 cut(s) | 1453 |
| PkrI | GCNGC | 5 cut(s) | 145, 693, 744, 820, 1213 |
| PleI | GAGTC | 2 cut(s) | 21, 909 |
| PluTI | GGCGCC | 1 cut(s) | 80 |
| PpsI | GAGTC | 2 cut(s) | 21, 909 |
| PpuMI | RGGWCCY | 1 cut(s) | 848 |
| Psp5II | RGGWCCY | 1 cut(s) | 848 |
| Psp6I | CCWGG | 1 cut(s) | 1453 |
| PspFI | CCCAGC | 2 cut(s) | 253, 1237 |
| PspGI | CCWGG | 1 cut(s) | 1453 |
| PspN4I | GGNNCC | 7 cut(s) | 6, 78, 603, 849, 850, 1114, 1392 |
| PspPI | GGNCC | 2 cut(s) | 493, 848 |
| PspPPI | RGGWCCY | 1 cut(s) | 848 |
| PstI | CTGCAG | 1 cut(s) | 823 |
| PsuI | RGATCY | 4 cut(s) | 187, 655, 864, 1330 |
| PvuII | CAGCTG | 1 cut(s) | 1223 |
| RsaI | GTAC | 7 cut(s) | 582, 937, 1153, 1158, 1192, 1343, 1351 |
| RsaNI | GTAC | 7 cut(s) | 581, 936, 1152, 1157, 1191, 1342, 1350 |
| RseI | CAYNNNNRTG | 1 cut(s) | 732 |
| SaqAI | TTAA | 5 cut(s) | 51, 1044, 1259, 1320, 1433 |
| SatI | GCNGC | 5 cut(s) | 144, 692, 743, 819, 1212 |
| Sau96I | GGNCC | 2 cut(s) | 493, 848 |
| ScaI | AGTACT | 2 cut(s) | 937, 1351 |
| SchI | GAGTC | 2 cut(s) | 22, 909 |
| ScrFI | CCNGG | 1 cut(s) | 1455 |
| SfaNI | GCATC | 3 cut(s) | 190, 253, 1256 |
| SfcI | CTRYAG | 1 cut(s) | 819 |
| SfoI | GGCGCC | 1 cut(s) | 78 |
| SinI | GGWCC | 2 cut(s) | 493, 848 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 732 |
| SmlI | CTYRAG | 2 cut(s) | 1345, 1395 |
| SmoI | CTYRAG | 2 cut(s) | 1345, 1395 |
| SsiI | CCGC | 6 cut(s) | 26, 143, 676, 1054, 1146, 1167 |
| SspDI | GGCGCC | 1 cut(s) | 76 |
| StyD4I | CCNGG | 1 cut(s) | 1453 |
| StyI | CCWWGG | 2 cut(s) | 422, 1011 |
| TaaI | ACNGT | 8 cut(s) | 109, 310, 580, 759, 776, 1138, 1156, 1426 |
| TaiI | ACGT | 1 cut(s) | 391 |
| TaqI | TCGA | 2 cut(s) | 876, 913 |
| TatI | WGTACW | 4 cut(s) | 935, 1190, 1341, 1349 |
| TauI | GCSGC | 1 cut(s) | 146 |
| TfiI | GAWTC | 2 cut(s) | 94, 1412 |
| Tru1I | TTAA | 5 cut(s) | 51, 1044, 1259, 1320, 1433 |
| Tru9I | TTAA | 5 cut(s) | 51, 1044, 1259, 1320, 1433 |
| TscAI | CASTG | 2 cut(s) | 114, 1468 |
| TseI | GCWGC | 4 cut(s) | 691, 742, 818, 1211 |
| TspDTI | ATGAA | 4 cut(s) | 289, 488, 1140, 1418 |
| TspGWI | ACGGA | 1 cut(s) | 37 |
| TspRI | CASTG | 2 cut(s) | 114, 1468 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1109 |
| VpaK11BI | GGWCC | 2 cut(s) | 493, 848 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 500 |
| XapI | RAATTY | 4 cut(s) | 347, 801, 1000, 1098 |
| XceI | RCATGY | 1 cut(s) | 749 |
| XmnI | GAANNNNTTC | 1 cut(s) | 801 |
| ZrmI | AGTACT | 2 cut(s) | 937, 1351 |