Rh4DG164300

Introduces a single-strand break via transesterification at a target site in duplex DNA. Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001026
Physical Location & Seq
Reverse (-)
32661700 .. 32685594
23895 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG164300.1

Sequence Viewer

Length: 1482 bp
ATGGCACCCCCGAGTCGCCTCCGACCGCACAACCCCAGTCCCCTCTCCGTTAAGGTCGGCATTGACTTGATGTCGGCGCCGTTAGTGTTGAAGATTCACCAGATCACAGTGCTTAGGCTTCCACCTCCACTTGACGAGTTCGCGGCCGTTGGATTTGTTGCTGGAAATGCAATTCCAGCCCTTGAAGATCTCCGTGCAGATGCAGGTGATCATCCTCCTGTTACTCCATTGAGGTCTGCAACTGAGGATATGCTGGGAAATGATGCGTGGAGATTATACGAGTATGTCTGTAAACACTTCATAGGGACTGTATCTCCTGACTGCAAGTATGTAAGAACAAAGGCAGAATTTTCAATTGGTGGAGAGTTCTTCCATTGTGTAGGACAACGTGTTTTGGTGAAGGGATTTACTTCTATTATGCCATGGTTAGCAGTAAATGAGAAAAATCTTCCTCAATTTACAAAGGGCGAGAAAATAGAAGTTGGCAGAGTGGACCTTCATGAGGGTATGACCTCACCTCCAGAATATCTAAGTGAGAGTGAGCTAATTTCGCTTATGGAGAAGAATGGAATCGGCACGGTACAAGCTGGCAGGAAGTTGGTTCCAACTGCCTTAGGTATTACTCTAATAAGAGGCTATCAATGTATTGATCCAGATCTGTGTTTGCCAGATATCCGCAGTTTTATTGAGCAGCAGATTACTCTTGTTGCTAAGGGTCAAGCAGATCATTTTCGTGTTGTGCAGCATGTTCTACAACAGTTCCGACAAAAATACAGTTATTTTGTAAAGCAGTTGGAAGAAATTCGTCAAAAGCGAGCTGCAGAAAGATTGAGCAAAACTTCTTCGGGACCGGATCTCAGCCAGATCCCACCTATCGATAATGGAGGAATGAGAAAATCGGAGAGCGCAAATCGACTCTCTAAGAGCGATGTTAGTACTTTAGTTTCTCAGATAAGAGACTTGGATAGGAAGAATGCAGAACTGGAAGAACAAAACAAAAATTTGGCTTCCATGGTGAGGTCTTGGTTGCCAAGTGGGAAGGTTAAGTCTGTGCGGTTGAATGATGTGAACTGGGGGATTAGTTGGAAAGATCGGCGAATTCCATTGCATGGAACCTTTGGAAATGAAGTTGCTACGGTGCTTGGCGGTGGTACTGTACCAGTTGGCGGTGATCCTAGTGCTGGCAAGAGTACACTTGTATTCCAGATTGCTGCACTTATAGCTGAAAGACATGAGCTGGGTAAAGCATCCCATGTTTTAAATGTCTCCGGTGAAGAGAGTAATGAGCAAATTGGGAGCAAAGCTGATCGTATGGAGATTAAAACAGAGGATCTTTTCTTGTACTCAAGTACTGATATTGAGGATATATTAGAGGAAAGTTGGTCTCTGGCTCCTCAAGCTCTAATTGCCGATTCCATTCAAACAGTTCATTTAAAAGGAGTGGCTGGAAGTCCTGGAGGCATTGTTCAGGGAACACGTTAA

Protein Analysis

493

Amino Acids

54.1

Weight (kDa)

6.48

Isoelectric Point (pI)

41.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Topoisom_bac PF01131 66 - 247 1.7e-34 DNA topoisomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 194
Acc36I ACCTGC 1 cut(s) 194
AccB1I GGYRCC 2 cut(s) 4, 76
AccB7I CCANNNNNTGG 1 cut(s) 1109
AccII CGCG 1 cut(s) 143
AciI CCGC 6 cut(s) 26, 143, 676, 1054, 1146, 1167
AclWI GGATC 5 cut(s) 644, 859, 861, 1166, 1338
AcoI YGGCCR 1 cut(s) 144
AcsI RAATTY 4 cut(s) 347, 801, 1000, 1098
AcyI GRCGYC 1 cut(s) 77
AfaI GTAC 7 cut(s) 582, 937, 1153, 1158, 1192, 1343, 1351
AfiI CCNNNNNNNGG 5 cut(s) 502, 1017, 1109, 1166, 1181
AflIII ACRYGT 2 cut(s) 388, 1475
AgsI TTSAA 5 cut(s) 91, 185, 354, 1060, 1421
AhdI GACNNNNNGTC 1 cut(s) 70
AjnI CCWGG 1 cut(s) 1453
AjuI GAANNNNNNNTTGG 2 cut(s) 339, 371
AloI GAACNNNNNNTCC 2 cut(s) 1449, 1481
AluBI AGCT 7 cut(s) 544, 587, 818, 1223, 1237, 1304, 1400
AluI AGCT 7 cut(s) 544, 587, 818, 1223, 1237, 1304, 1400
Alw26I GTCTC 3 cut(s) 951, 1270, 1389
AlwI GGATC 5 cut(s) 644, 859, 861, 1166, 1338
Ama87I CYCGRG 1 cut(s) 10
AoxI GGCC 1 cut(s) 144
ApeKI GCWGC 4 cut(s) 691, 742, 818, 1211
ApoI RAATTY 4 cut(s) 347, 801, 1000, 1098
Asp700I GAANNNNTTC 1 cut(s) 801
AspLEI GCGC 2 cut(s) 79, 908
AspS9I GGNCC 2 cut(s) 493, 848
AsuHPI GGTGA 7 cut(s) 89, 218, 409, 507, 1027, 1181, 1283
AvaI CYCGRG 1 cut(s) 10
AvaII GGWCC 2 cut(s) 493, 848
AxyI CCTNAGG 1 cut(s) 613
BanI GGYRCC 2 cut(s) 4, 76
BbvI GCAGC 4 cut(s) 703, 754, 805, 1198
BceAI ACGGC 2 cut(s) 64, 131
BciT130I CCWGG 1 cut(s) 1455
BclI TGATCA 1 cut(s) 208
BcoDI GTCTC 3 cut(s) 951, 1270, 1389
BfaI CTAG 1 cut(s) 1176
BfmI CTRYAG 1 cut(s) 819
BfoI RGCGCY 1 cut(s) 80
BfuAI ACCTGC 1 cut(s) 194
BglII AGATCT 2 cut(s) 187, 655
BisI GCNGC 5 cut(s) 144, 692, 743, 819, 1212
BlsI GCNGC 5 cut(s) 145, 693, 744, 820, 1213
BmcAI AGTACT 2 cut(s) 937, 1351
Bme1390I CCNGG 1 cut(s) 1455
Bme18I GGWCC 2 cut(s) 493, 848
BmeRI GACNNNNNGTC 1 cut(s) 70
BmeT110I CYCGRG 1 cut(s) 10
BmgT120I GGNCC 2 cut(s) 493, 848
BmiI GGNNCC 6 cut(s) 6, 78, 603, 849, 1114, 1392
BmrFI CCNGG 1 cut(s) 1455
BmrI ACTGGG 2 cut(s) 30, 1081
BmsI GCATC 3 cut(s) 190, 253, 1256
BmuI ACTGGG 2 cut(s) 30, 1081
BpmI CTGGAG 2 cut(s) 504, 1476
Bpu10I CCTNAGC 2 cut(s) 113, 711
BpuEI CTTGAG 2 cut(s) 1330, 1380
Bsa29I ATCGAT 1 cut(s) 876
BsaBI GATNNNNATC 1 cut(s) 654
BsaHI GRCGYC 1 cut(s) 77
BsaI GGTCTC 1 cut(s) 1389
BsaJI CCNNGG 2 cut(s) 422, 1011
BsaWI WCCGGW 2 cut(s) 850, 1268
BsaXI ACNNNNNCTCC 6 cut(s) 298, 328, 502, 532, 1449, 1479
Bsc4I CCNNNNNNNGG 5 cut(s) 502, 1017, 1109, 1166, 1181
Bse1I ACTGG 4 cut(s) 36, 987, 1076, 1160
Bse21I CCTNAGG 1 cut(s) 613
Bse3DI GCAATG 1 cut(s) 1103
Bse8I GATNNNNATC 1 cut(s) 654
BseBI CCWGG 1 cut(s) 1455
BseCI ATCGAT 1 cut(s) 876
BseDI CCNNGG 2 cut(s) 422, 1011
BseGI GGATG 2 cut(s) 211, 1247
BseJI GATNNNNATC 1 cut(s) 654
BseLI CCNNNNNNNGG 5 cut(s) 502, 1017, 1109, 1166, 1181
BseMI GCAATG 1 cut(s) 1103
BseMII CTCAG 3 cut(s) 234, 871, 962
BseNI ACTGG 4 cut(s) 36, 987, 1076, 1160
BseRI GAGGAG 1 cut(s) 1383
BseX3I CGGCCG 1 cut(s) 144
BseXI GCAGC 4 cut(s) 703, 754, 805, 1198
BseYI CCCAGC 2 cut(s) 253, 1237
BsgI GTGCAG 3 cut(s) 216, 761, 1197
Bsh1236I CGCG 1 cut(s) 143
Bsh1285I CGRYCG 2 cut(s) 26, 147
BshFI GGCC 1 cut(s) 146
BshNI GGYRCC 2 cut(s) 4, 76
BshVI ATCGAT 1 cut(s) 876
BsiEI CGRYCG 2 cut(s) 26, 147
BsiHKCI CYCGRG 1 cut(s) 10
BsiSI CCGG 2 cut(s) 851, 1269
BslFI GGGAC 3 cut(s) 24, 319, 861
BslI CCNNNNNNNGG 5 cut(s) 502, 1017, 1109, 1166, 1181
BsmAI GTCTC 3 cut(s) 951, 1270, 1389
BsmFI GGGAC 3 cut(s) 24, 319, 861
BsmI GAATGC 1 cut(s) 979
BsnI GGCC 1 cut(s) 146
Bso31I GGTCTC 1 cut(s) 1389
BsoBI CYCGRG 1 cut(s) 10
Bsp19I CCATGG 2 cut(s) 422, 1011
BspACI CCGC 6 cut(s) 26, 143, 676, 1054, 1146, 1167
BspANI GGCC 1 cut(s) 146
BspCNI CTCAG 3 cut(s) 235, 870, 961
BspDI ATCGAT 1 cut(s) 876
BspFNI CGCG 1 cut(s) 143
BspHI TCATGA 1 cut(s) 499
BspLI GGNNCC 6 cut(s) 6, 78, 603, 849, 1114, 1392
BspMAI CTGCAG 1 cut(s) 823
BspMI ACCTGC 1 cut(s) 194
BspPI GGATC 5 cut(s) 644, 859, 861, 1166, 1338
BspT107I GGYRCC 2 cut(s) 4, 76
BspTNI GGTCTC 1 cut(s) 1389
BsrDI GCAATG 1 cut(s) 1103
BsrI ACTGG 4 cut(s) 36, 987, 1076, 1160
BssECI CCNNGG 2 cut(s) 422, 1011
BssNI GRCGYC 1 cut(s) 77
BssT1I CCWWGG 2 cut(s) 422, 1011
Bst2UI CCWGG 1 cut(s) 1455
Bst4CI ACNGT 8 cut(s) 109, 310, 580, 759, 776, 1138, 1156, 1426
Bst6I CTCTTC 1 cut(s) 1269
BstACI GRCGYC 1 cut(s) 77
BstC8I GCNNGC 3 cut(s) 589, 816, 1183
BstDEI CTNAG 8 cut(s) 113, 243, 530, 613, 711, 857, 921, 948
BstDSI CCRYGG 2 cut(s) 422, 1011
BstENI CCTNNNNNAGG 1 cut(s) 500
BstF5I GGATG 2 cut(s) 211, 1247
BstFNI CGCG 1 cut(s) 143
BstH2I RGCGCY 1 cut(s) 80
BstHHI GCGC 2 cut(s) 79, 908
BstMAI GTCTC 3 cut(s) 951, 1270, 1389
BstMCI CGRYCG 2 cut(s) 26, 147
BstMWI GCNNNNNNNGC 6 cut(s) 167, 176, 550, 1220, 1397, 1406
BstNI CCWGG 1 cut(s) 1455
BstNSI RCATGY 1 cut(s) 749
BstSCI CCNGG 1 cut(s) 1453
BstSFI CTRYAG 1 cut(s) 819
BstUI CGCG 1 cut(s) 143
BstV1I GCAGC 4 cut(s) 703, 754, 805, 1198
BstX2I RGATCY 5 cut(s) 187, 655, 853, 864, 1330
BstYI RGATCY 5 cut(s) 187, 655, 853, 864, 1330
BstZI CGGCCG 1 cut(s) 144
Bsu15I ATCGAT 1 cut(s) 876
Bsu36I CCTNAGG 1 cut(s) 613
BsuRI GGCC 1 cut(s) 146
BsuTUI ATCGAT 1 cut(s) 876
BtgI CCRYGG 2 cut(s) 422, 1011
BtgZI GCGATG 1 cut(s) 942
BtsCI GGATG 2 cut(s) 211, 1247
BtsIMutI CAGTG 1 cut(s) 114
BveI ACCTGC 1 cut(s) 194
Cac8I GCNNGC 3 cut(s) 589, 816, 1183
CciI TCATGA 1 cut(s) 499
CfoI GCGC 2 cut(s) 79, 908
Cfr13I GGNCC 2 cut(s) 493, 848
ClaI ATCGAT 1 cut(s) 876
Csp6I GTAC 7 cut(s) 581, 936, 1152, 1157, 1191, 1342, 1350
CviAII CATG 7 cut(s) 423, 500, 746, 1012, 1109, 1232, 1253
CviQI GTAC 7 cut(s) 581, 936, 1152, 1157, 1191, 1342, 1350
DdeI CTNAG 8 cut(s) 113, 243, 530, 613, 711, 857, 921, 948
DinI GGCGCC 1 cut(s) 78
DraI TTTAAA 2 cut(s) 1260, 1434
DriI GACNNNNNGTC 1 cut(s) 70
EaeI YGGCCR 1 cut(s) 144
EagI CGGCCG 1 cut(s) 144
Eam1104I CTCTTC 1 cut(s) 1269
Eam1105I GACNNNNNGTC 1 cut(s) 70
EarI CTCTTC 1 cut(s) 1269
EclXI CGGCCG 1 cut(s) 144
Eco130I CCWWGG 2 cut(s) 422, 1011
Eco31I GGTCTC 1 cut(s) 1389
Eco32I GATATC 1 cut(s) 673
Eco47I GGWCC 2 cut(s) 493, 848
Eco52I CGGCCG 1 cut(s) 144
Eco81I CCTNAGG 1 cut(s) 613
Eco88I CYCGRG 1 cut(s) 10
EcoNI CCTNNNNNAGG 1 cut(s) 500
EcoRI GAATTC 1 cut(s) 1098
EcoRII CCWGG 1 cut(s) 1453
EcoRV GATATC 1 cut(s) 673
EcoT14I CCWWGG 2 cut(s) 422, 1011
EgeI GGCGCC 1 cut(s) 78
EheI GGCGCC 1 cut(s) 78
ErhI CCWWGG 2 cut(s) 422, 1011
FaeI CATG 7 cut(s) 426, 503, 749, 1015, 1112, 1235, 1256
FaqI GGGAC 3 cut(s) 24, 319, 861
FatI CATG 7 cut(s) 422, 499, 745, 1011, 1108, 1231, 1252
FbaI TGATCA 1 cut(s) 208
Fnu4HI GCNGC 5 cut(s) 144, 692, 743, 819, 1212
FokI GGATG 2 cut(s) 198, 1234
Fsp4HI GCNGC 5 cut(s) 144, 692, 743, 819, 1212
FspBI CTAG 1 cut(s) 1176
GlaI GCGC 2 cut(s) 78, 907
GluI GCNGC 5 cut(s) 144, 692, 743, 819, 1212
GsaI CCCAGC 2 cut(s) 257, 1241
GsuI CTGGAG 2 cut(s) 504, 1476
HaeII RGCGCY 1 cut(s) 80
HaeIII GGCC 1 cut(s) 146
HapII CCGG 2 cut(s) 851, 1269
HhaI GCGC 2 cut(s) 79, 908
Hin1I GRCGYC 1 cut(s) 77
Hin1II CATG 7 cut(s) 426, 503, 749, 1015, 1112, 1235, 1256
Hin6I GCGC 2 cut(s) 77, 906
HinP1I GCGC 2 cut(s) 77, 906
HinfI GANTC 5 cut(s) 13, 94, 570, 915, 1412
HpaII CCGG 2 cut(s) 851, 1269
HphI GGTGA 7 cut(s) 89, 218, 409, 507, 1027, 1181, 1283
Hpy166II GTNNAC 4 cut(s) 293, 493, 1069, 1193
Hpy188I TCNGA 4 cut(s) 23, 764, 901, 951
Hpy188III TCNNGA 6 cut(s) 317, 500, 521, 653, 846, 1204
Hpy8I GTNNAC 4 cut(s) 293, 493, 1069, 1193
HpyAV CCTTC 3 cut(s) 394, 506, 1033
HpyCH4III ACNGT 8 cut(s) 109, 310, 580, 759, 776, 1138, 1156, 1426
HpyCH4IV ACGT 2 cut(s) 388, 1477
HpyF10VI GCNNNNNNNGC 6 cut(s) 167, 176, 550, 1220, 1397, 1406
HpyF3I CTNAG 8 cut(s) 113, 243, 530, 613, 711, 857, 921, 948
HpySE526I ACGT 2 cut(s) 388, 1477
Hsp92I GRCGYC 1 cut(s) 77
Hsp92II CATG 7 cut(s) 426, 503, 749, 1015, 1112, 1235, 1256
HspAI GCGC 2 cut(s) 77, 906
KasI GGCGCC 1 cut(s) 76
Ksp22I TGATCA 1 cut(s) 208
LmnI GCTCC 2 cut(s) 1296, 1396
Lsp1109I GCAGC 4 cut(s) 703, 754, 805, 1198
LweI GCATC 3 cut(s) 190, 253, 1256
MaeI CTAG 1 cut(s) 1176
MaeII ACGT 2 cut(s) 388, 1477
MaeIII GTNAC 1 cut(s) 220
MfeI CAATTG 1 cut(s) 354
MflI RGATCY 5 cut(s) 187, 655, 853, 864, 1330
Mly113I GGCGCC 1 cut(s) 77
MlyI GAGTC 2 cut(s) 22, 909
MmeI TCCRAC 6 cut(s) 46, 130, 629, 774, 787, 1064
MroXI GAANNNNTTC 1 cut(s) 801
MseI TTAA 6 cut(s) 51, 1044, 1259, 1320, 1433, 1480
MslI CAYNNNNRTG 1 cut(s) 732
MspI CCGG 2 cut(s) 851, 1269
MspR9I CCNGG 1 cut(s) 1455
MunI CAATTG 1 cut(s) 354
Mva1269I GAATGC 1 cut(s) 979
MvaI CCWGG 1 cut(s) 1455
MvnI CGCG 1 cut(s) 143
MwoI GCNNNNNNNGC 6 cut(s) 167, 176, 550, 1220, 1397, 1406
NarI GGCGCC 1 cut(s) 77
NcoI CCATGG 2 cut(s) 422, 1011
NlaIII CATG 7 cut(s) 426, 503, 749, 1015, 1112, 1235, 1256
NlaIV GGNNCC 6 cut(s) 6, 78, 603, 849, 1114, 1392
NspI RCATGY 1 cut(s) 749
PagI TCATGA 1 cut(s) 499
PaqCI CACCTGC 1 cut(s) 194
PcsI WCGNNNNNNNCGW 1 cut(s) 811
PctI GAATGC 1 cut(s) 979
PdmI GAANNNNTTC 1 cut(s) 801
PfeI GAWTC 3 cut(s) 94, 570, 1412
PflMI CCANNNNNTGG 1 cut(s) 1109
PfoI TCCNGGA 1 cut(s) 1453
PkrI GCNGC 5 cut(s) 145, 693, 744, 820, 1213
PleI GAGTC 2 cut(s) 21, 909
PluTI GGCGCC 1 cut(s) 80
PpsI GAGTC 2 cut(s) 21, 909
Psp6I CCWGG 1 cut(s) 1453
PspFI CCCAGC 2 cut(s) 253, 1237
PspGI CCWGG 1 cut(s) 1453
PspN4I GGNNCC 6 cut(s) 6, 78, 603, 849, 1114, 1392
PspPI GGNCC 2 cut(s) 493, 848
PstI CTGCAG 1 cut(s) 823
PsuI RGATCY 5 cut(s) 187, 655, 853, 864, 1330
RsaI GTAC 7 cut(s) 582, 937, 1153, 1158, 1192, 1343, 1351
RsaNI GTAC 7 cut(s) 581, 936, 1152, 1157, 1191, 1342, 1350
RseI CAYNNNNRTG 1 cut(s) 732
SaqAI TTAA 6 cut(s) 51, 1044, 1259, 1320, 1433, 1480
SatI GCNGC 5 cut(s) 144, 692, 743, 819, 1212
Sau96I GGNCC 2 cut(s) 493, 848
ScaI AGTACT 2 cut(s) 937, 1351
SchI GAGTC 2 cut(s) 22, 909
ScrFI CCNGG 1 cut(s) 1455
SfaNI GCATC 3 cut(s) 190, 253, 1256
SfcI CTRYAG 1 cut(s) 819
SfoI GGCGCC 1 cut(s) 78
SinI GGWCC 2 cut(s) 493, 848
SmiMI CAYNNNNRTG 1 cut(s) 732
SmlI CTYRAG 2 cut(s) 1345, 1395
SmoI CTYRAG 2 cut(s) 1345, 1395
SsiI CCGC 6 cut(s) 26, 143, 676, 1054, 1146, 1167
SspDI GGCGCC 1 cut(s) 76
SspMI CTAG 1 cut(s) 1176
StyD4I CCNGG 1 cut(s) 1453
StyI CCWWGG 2 cut(s) 422, 1011
TaaI ACNGT 8 cut(s) 109, 310, 580, 759, 776, 1138, 1156, 1426
TaiI ACGT 2 cut(s) 391, 1480
TaqI TCGA 2 cut(s) 876, 913
TatI WGTACW 4 cut(s) 935, 1190, 1341, 1349
TauI GCSGC 1 cut(s) 146
TfiI GAWTC 3 cut(s) 94, 570, 1412
Tru1I TTAA 6 cut(s) 51, 1044, 1259, 1320, 1433, 1480
Tru9I TTAA 6 cut(s) 51, 1044, 1259, 1320, 1433, 1480
TscAI CASTG 1 cut(s) 114
TseI GCWGC 4 cut(s) 691, 742, 818, 1211
TspDTI ATGAA 4 cut(s) 289, 488, 1140, 1418
TspGWI ACGGA 2 cut(s) 37, 182
TspRI CASTG 1 cut(s) 114
Van91I CCANNNNNTGG 1 cut(s) 1109
VpaK11BI GGWCC 2 cut(s) 493, 848
XagI CCTNNNNNAGG 1 cut(s) 500
XapI RAATTY 4 cut(s) 347, 801, 1000, 1098
XceI RCATGY 1 cut(s) 749
XmnI GAANNNNTTC 1 cut(s) 801
XspI CTAG 1 cut(s) 1176
ZrmI AGTACT 2 cut(s) 937, 1351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.