AT5G54710

protein ubiquitination

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001058
Physical Location & Seq
Reverse (-)
22227483 .. 22230663
3181 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G54710.2

Sequence Viewer

Length: 1797 bp
ATGAAGATTAATAAGCAAGGACAAACAGCATTCATATTAGCATGCCTCAACAATCACCTCGATGCTGCACTTACCCTCGTAGAAAATATGCGGTCTATGACGATGGTGGAACTTGATGCAGCCTTTACAGGACAGCAACCAGTTATCATAGATAAAATGCTGGAGAAATTCCCGAGCCTAGTTCTTGATGTGGATGAAGAGCAGTCGACGCTATTGCATAAAGCCGTGACACAAAGGAACGAGGAATATGCGACGAAGGTAATCGACCTCTGCCCATCACTCGTTAGCGTTACTAACGTCGACGGAAACACTCCTCTTCATTTAGCTGCTGAAATTGGAAACATAAATATCCTCTGGAAAATGTTGGAGACCGGAGAAGCAGAGTGCATGAAGATTAATAAGCAAGGACAAACAGCATTCATATTAGCATGCCTCAACAATAACGTTAATTCTGCCAGAATCCTTGTAGAAGGAACGAGTTCTATGACGATGGTGGAACTTAATGCAGCCTTTTCAGAACAGCAACAAGTTATCATAGATTCCATACTAGAGAAATTCCCGAACCTAATTCTTGATGCTGATGAAGAGCAGTCGACGCTATTGCATAAAGCGTGCAAATCAGGAAACCTTGAGATGGCAAGGACACTATTAGATGTTGATGTTAATCAAGAAATTGCAGAAAAGGTGGATAAAGATGGTTTGACCCCTTTGCATCGCGCGGTGATAAACGGTTCAGTTGAAATCCTAAAGGAGTTTCTATGCAAGGCTCCTTCGTCTTTCAACATAACCACACAAGGAACAATCGAAACAGTTTTCCACTTAGCTGCGAAATACCAAAAAACCAAAGCATTCATCTTCATGGCCCAGAGTGCAAACATACGCCAACTTCTTTACAGCCTCGACGCAGAGGACAACACTGTACTTCATGTTGCCGCCTCCGTAGACTCCACTTCTCTAGTACGCCACATTCTTTCGGAAACAACAATTGATGTCACCTTGAAAAACAAGAAAGGTTTTGCAGCCGTTGACCTAATCGACAAAGAGGGTGTAGACTTTCCTCTACTCTCTCTATGGTTCCGGGATGAAGCAGAAAAAATTCAACGCCCTGCGAGGTATGTTAAATTTGCACATGAACCTGTAGAACTCATCCGAAACACAAACAATGGGGAGAAGTTAAGTTCTGAAAGTCGTGCGATGGATTTGCTAAGAGAAGGTAGAGATCCTAGAAACAAGGAACGTGAAATGCATTCAGAGAGTCTACAGAACGCAAGGAACACTATTACTATTGTCGCTGTCTTGATTGCATCGGTTGCTTTCACATGCGGGATAAACCCTCCTGGTGGTGTCCACCAAGACGGACCCTTCATCGGGAAAGCAACCGCAGGGAGAACATTGGCTTTTAAGATCTTCTCGGTAGCAAACAACATTGCTTTGTTTACATCACTAAGTATCGTCACTCTTCTGGTCAGCATTATATCCTATAGAACGAAAGCACTGAAGATGTGTGTGGTCATAGCACATAAGATGATGTGGTTGGCCGTGGCATCCATGGCAACTGCTTATGCTGCATCGGCATGGATCACTGTGCCACATAACGAGGGATCTAAATGGTTAGTCTACACCACTTCTGCTATCGCTAGCGTTGCGCTAGGAAGTATGTTTGTGTATGTGTCATTTATGATGGTTAAACACATTTTGAAGAAGGATAAATTGAGACGTAACCAGTCTCACGGGAAAACCCCAAGTACGACCTTGGACATGGAAGCCGCTGGTGATGTCGGCTGCTATTGGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank Ank_2 Ank_3 Ank_4 Ank_5 PGG

Protein Analysis

598

Amino Acids

66.04

Weight (kDa)

6.14

Isoelectric Point (pI)

43.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 12 - 74 6.5e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 117 - 202 3.8e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 236 - 300 1e-11 Ankyrin repeats (3 copies)
Ank_4 PF13637 246 - 295 1.1e-09 Ankyrin repeats (many copies)
PGG PF13962 463 - 572 4.3e-29 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 7 cut(s) 206, 300, 593, 942, 1050, 1258, 1617
AccII CGCG 2 cut(s) 717, 719
AciI CCGC 6 cut(s) 91, 719, 933, 1323, 1380, 1767
AclI AACGTT 1 cut(s) 444
AclWI GGATC 3 cut(s) 1214, 1586, 1609
AcoI YGGCCR 1 cut(s) 1536
AcsI RAATTY 4 cut(s) 167, 554, 1095, 1121
AcuI CTGAAG 1 cut(s) 1517
AfaI GTAC 3 cut(s) 921, 960, 1747
AfiI CCNNNNNNNGG 4 cut(s) 634, 1340, 1367, 1368
AgsI TTSAA 5 cut(s) 740, 781, 1000, 1100, 1699
AjnI CCWGG 1 cut(s) 1336
AluBI AGCT 2 cut(s) 326, 824
AluI AGCT 2 cut(s) 326, 824
Alw26I GTCTC 3 cut(s) 362, 1708, 1731
AlwI GGATC 3 cut(s) 1214, 1586, 1609
Ama87I CYCGRG 1 cut(s) 172
AoxI GGCC 2 cut(s) 861, 1536
ApeKI GCWGC 8 cut(s) 65, 119, 326, 506, 824, 1019, 1565, 1782
ApoI RAATTY 4 cut(s) 167, 554, 1095, 1121
AseI ATTAAT 2 cut(s) 9, 396
Asp700I GAANNNNTTC 2 cut(s) 478, 1095
AspLEI GCGC 2 cut(s) 719, 1648
AspS9I GGNCC 2 cut(s) 862, 1358
AsuC2I CCSGG 1 cut(s) 1079
AsuHPI GGTGA 4 cut(s) 47, 733, 985, 1784
AsuNHI GCTAGC 1 cut(s) 1637
AvaI CYCGRG 1 cut(s) 172
AvaII GGWCC 1 cut(s) 1358
BbvI GCAGC 8 cut(s) 52, 131, 313, 518, 811, 1031, 1552, 1769
BccI CCATC 7 cut(s) 97, 283, 484, 628, 689, 1189, 1675
BceAI ACGGC 3 cut(s) 209, 1007, 1523
BcgI CGANNNNNNTGC 8 cut(s) 196, 230, 230, 264, 583, 617, 1183, 1217
BciT130I CCWGG 1 cut(s) 1338
BcnI CCSGG 1 cut(s) 1079
BcoDI GTCTC 3 cut(s) 362, 1708, 1731
BfaI CTAG 6 cut(s) 179, 548, 956, 1224, 1638, 1649
BfmI CTRYAG 3 cut(s) 1137, 1259, 1480
BglII AGATCT 1 cut(s) 1404
Bme1390I CCNGG 2 cut(s) 1079, 1338
Bme18I GGWCC 1 cut(s) 1358
BmeT110I CYCGRG 1 cut(s) 172
BmgT120I GGNCC 2 cut(s) 862, 1358
BmiI GGNNCC 3 cut(s) 768, 1076, 1360
BmrFI CCNGG 2 cut(s) 1079, 1338
BmsI GCATC 7 cut(s) 52, 106, 565, 721, 1313, 1553, 1577
BmtI GCTAGC 1 cut(s) 1641
BpmI CTGGAG 1 cut(s) 182
BpuEI CTTGAG 1 cut(s) 650
BpuMI CCSGG 1 cut(s) 1079
BsaBI GATNNNNATC 1 cut(s) 663
BsaI GGTCTC 1 cut(s) 362
BsaJI CCNNGG 3 cut(s) 1539, 1548, 1752
BsaWI WCCGGW 1 cut(s) 371
Bsc4I CCNNNNNNNGG 4 cut(s) 634, 1340, 1367, 1368
Bse1I ACTGG 2 cut(s) 140, 1723
Bse3DI GCAATG 1 cut(s) 1425
Bse8I GATNNNNATC 1 cut(s) 663
BseBI CCWGG 1 cut(s) 1338
BseDI CCNNGG 3 cut(s) 1539, 1548, 1752
BseGI GGATG 4 cut(s) 199, 1087, 1146, 1544
BseJI GATNNNNATC 1 cut(s) 663
BseLI CCNNNNNNNGG 4 cut(s) 634, 1340, 1367, 1368
BseMI GCAATG 1 cut(s) 1425
BseNI ACTGG 2 cut(s) 140, 1723
BseRI GAGGAG 1 cut(s) 303
BseXI GCAGC 8 cut(s) 52, 131, 313, 518, 811, 1031, 1552, 1769
BsgI GTGCAG 1 cut(s) 51
Bsh1236I CGCG 2 cut(s) 717, 719
BshFI GGCC 2 cut(s) 863, 1538
BsiHKCI CYCGRG 1 cut(s) 172
BsiSI CCGG 2 cut(s) 372, 1078
BslI CCNNNNNNNGG 4 cut(s) 634, 1340, 1367, 1368
BsmAI GTCTC 3 cut(s) 362, 1708, 1731
BsmBI CGTCTC 1 cut(s) 1708
BsmI GAATGC 4 cut(s) 29, 416, 848, 1246
BsnI GGCC 2 cut(s) 863, 1538
Bso31I GGTCTC 1 cut(s) 362
BsoBI CYCGRG 1 cut(s) 172
Bsp143I GATC 4 cut(s) 1219, 1404, 1578, 1601
Bsp19I CCATGG 1 cut(s) 1548
BspACI CCGC 6 cut(s) 91, 719, 933, 1323, 1380, 1767
BspANI GGCC 2 cut(s) 863, 1538
BspFNI CGCG 2 cut(s) 717, 719
BspLI GGNNCC 3 cut(s) 768, 1076, 1360
BspOI GCTAGC 1 cut(s) 1641
BspPI GGATC 3 cut(s) 1214, 1586, 1609
BspQI GCTCTTC 2 cut(s) 192, 579
BspTNI GGTCTC 1 cut(s) 362
BsrDI GCAATG 1 cut(s) 1425
BsrI ACTGG 2 cut(s) 140, 1723
BssECI CCNNGG 3 cut(s) 1539, 1548, 1752
BssMI GATC 4 cut(s) 1219, 1404, 1578, 1601
BssT1I CCWWGG 2 cut(s) 1548, 1752
Bst2UI CCWGG 1 cut(s) 1338
Bst4CI ACNGT 4 cut(s) 731, 811, 919, 1585
Bst6I CTCTTC 4 cut(s) 192, 321, 579, 1464
BstAPI GCANNNNNTGC 1 cut(s) 1310
BstC8I GCNNGC 4 cut(s) 43, 430, 613, 1639
BstDEI CTNAG 3 cut(s) 820, 1205, 1445
BstDSI CCRYGG 2 cut(s) 1539, 1548
BstF5I GGATG 4 cut(s) 199, 1087, 1146, 1544
BstFNI CGCG 2 cut(s) 717, 719
BstHHI GCGC 2 cut(s) 719, 1648
BstKTI GATC 4 cut(s) 1222, 1407, 1581, 1604
BstMAI GTCTC 3 cut(s) 362, 1708, 1731
BstMBI GATC 4 cut(s) 1219, 1404, 1578, 1601
BstMWI GCNNNNNNNGC 8 cut(s) 208, 595, 869, 1310, 1550, 1565, 1571, 1643
BstNI CCWGG 1 cut(s) 1338
BstNSI RCATGY 3 cut(s) 45, 432, 1323
BstSCI CCNGG 2 cut(s) 1077, 1336
BstSFI CTRYAG 3 cut(s) 1137, 1259, 1480
BstUI CGCG 2 cut(s) 717, 719
BstV1I GCAGC 8 cut(s) 52, 131, 313, 518, 811, 1031, 1552, 1769
BstX2I RGATCY 3 cut(s) 1219, 1404, 1601
BstYI RGATCY 3 cut(s) 1219, 1404, 1601
BsuRI GGCC 2 cut(s) 863, 1538
BtgI CCRYGG 2 cut(s) 1539, 1548
BtgZI GCGATG 2 cut(s) 698, 1208
BtsCI GGATG 4 cut(s) 199, 1087, 1146, 1544
BtsIMutI CAGTG 3 cut(s) 915, 1493, 1581
Cac8I GCNNGC 4 cut(s) 43, 430, 613, 1639
CfoI GCGC 2 cut(s) 719, 1648
Cfr13I GGNCC 2 cut(s) 862, 1358
CseI GACGC 3 cut(s) 217, 604, 911
Csp6I GTAC 3 cut(s) 920, 959, 1746
CviQI GTAC 3 cut(s) 920, 959, 1746
DdeI CTNAG 3 cut(s) 820, 1205, 1445
DpnI GATC 4 cut(s) 1221, 1406, 1580, 1603
DpnII GATC 4 cut(s) 1219, 1404, 1578, 1601
EaeI YGGCCR 1 cut(s) 1536
Eam1104I CTCTTC 4 cut(s) 192, 321, 579, 1464
EarI CTCTTC 4 cut(s) 192, 321, 579, 1464
Eco130I CCWWGG 2 cut(s) 1548, 1752
Eco31I GGTCTC 1 cut(s) 362
Eco47I GGWCC 1 cut(s) 1358
Eco57I CTGAAG 1 cut(s) 1517
Eco88I CYCGRG 1 cut(s) 172
EcoRII CCWGG 1 cut(s) 1336
EcoT14I CCWWGG 2 cut(s) 1548, 1752
EcoT22I ATGCAT 1 cut(s) 1248
ErhI CCWWGG 2 cut(s) 1548, 1752
Esp3I CGTCTC 1 cut(s) 1708
FauI CCCGC 1 cut(s) 1316
FblI GTMKAC 7 cut(s) 206, 300, 593, 942, 1050, 1258, 1617
FokI GGATG 4 cut(s) 206, 1094, 1133, 1531
FspBI CTAG 6 cut(s) 179, 548, 956, 1224, 1638, 1649
GlaI GCGC 2 cut(s) 718, 1647
GsuI CTGGAG 1 cut(s) 182
HaeIII GGCC 2 cut(s) 863, 1538
HapII CCGG 2 cut(s) 372, 1078
HgaI GACGC 3 cut(s) 217, 604, 911
HhaI GCGC 2 cut(s) 719, 1648
Hin6I GCGC 2 cut(s) 717, 1646
HinP1I GCGC 2 cut(s) 717, 1646
HincII GTYRAC 4 cut(s) 207, 301, 594, 1027
HindII GTYRAC 4 cut(s) 207, 301, 594, 1027
HinfI GANTC 4 cut(s) 459, 539, 944, 1255
HpaII CCGG 2 cut(s) 372, 1078
HphI GGTGA 4 cut(s) 47, 733, 985, 1784
Hpy188I TCNGA 5 cut(s) 517, 976, 1151, 1183, 1252
Hpy188III TCNNGA 9 cut(s) 172, 185, 355, 559, 572, 621, 668, 1297, 1369
Hpy99I CGWCG 6 cut(s) 211, 256, 302, 305, 598, 905
HpyAV CCTTC 6 cut(s) 250, 464, 780, 1205, 1372, 1696
HpyCH4III ACNGT 4 cut(s) 731, 811, 919, 1585
HpyCH4IV ACGT 4 cut(s) 297, 444, 1237, 1717
HpyF10VI GCNNNNNNNGC 8 cut(s) 208, 595, 869, 1310, 1550, 1565, 1571, 1643
HpyF3I CTNAG 3 cut(s) 820, 1205, 1445
HpySE526I ACGT 4 cut(s) 297, 444, 1237, 1717
HspAI GCGC 2 cut(s) 717, 1646
Kzo9I GATC 4 cut(s) 1219, 1404, 1578, 1601
LguI GCTCTTC 2 cut(s) 192, 579
LmnI GCTCC 1 cut(s) 772
Lsp1109I GCAGC 8 cut(s) 52, 131, 313, 518, 811, 1031, 1552, 1769
LweI GCATC 7 cut(s) 52, 106, 565, 721, 1313, 1553, 1577
MaeI CTAG 6 cut(s) 179, 548, 956, 1224, 1638, 1649
MaeII ACGT 4 cut(s) 297, 444, 1237, 1717
MaeIII GTNAC 5 cut(s) 226, 289, 991, 1453, 1718
MalI GATC 4 cut(s) 1221, 1406, 1580, 1603
MboI GATC 4 cut(s) 1219, 1404, 1578, 1601
MfeI CAATTG 1 cut(s) 984
MflI RGATCY 3 cut(s) 1219, 1404, 1601
MlyI GAGTC 2 cut(s) 938, 1264
MmeI TCCRAC 1 cut(s) 345
Mph1103I ATGCAT 1 cut(s) 1248
MroXI GAANNNNTTC 2 cut(s) 478, 1095
MseI TTAA 9 cut(s) 9, 396, 447, 501, 663, 1119, 1175, 1401, 1686
MslI CAYNNNNRTG 3 cut(s) 60, 857, 1573
MspA1I CMGCKG 1 cut(s) 1769
MspI CCGG 2 cut(s) 372, 1078
MspR9I CCNGG 2 cut(s) 1079, 1338
MunI CAATTG 1 cut(s) 984
Mva1269I GAATGC 4 cut(s) 29, 416, 848, 1246
MvaI CCWGG 1 cut(s) 1338
MvnI CGCG 2 cut(s) 717, 719
MwoI GCNNNNNNNGC 8 cut(s) 208, 595, 869, 1310, 1550, 1565, 1571, 1643
NciI CCSGG 1 cut(s) 1079
NcoI CCATGG 1 cut(s) 1548
NdeII GATC 4 cut(s) 1219, 1404, 1578, 1601
NheI GCTAGC 1 cut(s) 1637
NlaIV GGNNCC 3 cut(s) 768, 1076, 1360
NmuCI GTSAC 3 cut(s) 226, 991, 1453
NsiI ATGCAT 1 cut(s) 1248
NspI RCATGY 3 cut(s) 45, 432, 1323
PaeI GCATGC 2 cut(s) 45, 432
PciSI GCTCTTC 2 cut(s) 192, 579
PctI GAATGC 4 cut(s) 29, 416, 848, 1246
PdmI GAANNNNTTC 2 cut(s) 478, 1095
PfeI GAWTC 2 cut(s) 459, 539
PfoI TCCNGGA 1 cut(s) 1077
PleI GAGTC 2 cut(s) 938, 1263
PpsI GAGTC 2 cut(s) 938, 1263
PshBI ATTAAT 2 cut(s) 9, 396
Psp1406I AACGTT 1 cut(s) 444
Psp6I CCWGG 1 cut(s) 1336
PspGI CCWGG 1 cut(s) 1336
PspN4I GGNNCC 3 cut(s) 768, 1076, 1360
PspPI GGNCC 2 cut(s) 862, 1358
PsuI RGATCY 3 cut(s) 1219, 1404, 1601
RsaI GTAC 3 cut(s) 921, 960, 1747
RsaNI GTAC 3 cut(s) 920, 959, 1746
RseI CAYNNNNRTG 3 cut(s) 60, 857, 1573
SalI GTCGAC 3 cut(s) 205, 299, 592
SapI GCTCTTC 2 cut(s) 192, 579
SaqAI TTAA 9 cut(s) 9, 396, 447, 501, 663, 1119, 1175, 1401, 1686
Sau3AI GATC 4 cut(s) 1219, 1404, 1578, 1601
Sau96I GGNCC 2 cut(s) 862, 1358
SchI GAGTC 2 cut(s) 938, 1264
ScrFI CCNGG 2 cut(s) 1079, 1338
SfaNI GCATC 7 cut(s) 52, 106, 565, 721, 1313, 1553, 1577
SfcI CTRYAG 3 cut(s) 1137, 1259, 1480
SgrDI CGTCGACG 1 cut(s) 299
SinI GGWCC 1 cut(s) 1358
SmiMI CAYNNNNRTG 3 cut(s) 60, 857, 1573
SmlI CTYRAG 1 cut(s) 629
SmoI CTYRAG 1 cut(s) 629
SphI GCATGC 2 cut(s) 45, 432
SsiI CCGC 6 cut(s) 91, 719, 933, 1323, 1380, 1767
SspMI CTAG 6 cut(s) 179, 548, 956, 1224, 1638, 1649
StyD4I CCNGG 2 cut(s) 1077, 1336
StyI CCWWGG 2 cut(s) 1548, 1752
TaaI ACNGT 4 cut(s) 731, 811, 919, 1585
TaiI ACGT 4 cut(s) 300, 447, 1240, 1720
TaqI TCGA 8 cut(s) 60, 206, 264, 300, 593, 804, 900, 1035
TatI WGTACW 1 cut(s) 919
TauI GCSGC 2 cut(s) 935, 1769
TfiI GAWTC 2 cut(s) 459, 539
Tru1I TTAA 9 cut(s) 9, 396, 447, 501, 663, 1119, 1175, 1401, 1686
Tru9I TTAA 9 cut(s) 9, 396, 447, 501, 663, 1119, 1175, 1401, 1686
TscAI CASTG 3 cut(s) 922, 1500, 1588
TseFI GTSAC 3 cut(s) 226, 991, 1453
TseI GCWGC 8 cut(s) 65, 119, 326, 506, 824, 1019, 1565, 1782
Tsp45I GTSAC 3 cut(s) 226, 991, 1453
TspGWI ACGGA 3 cut(s) 318, 928, 1371
TspRI CASTG 3 cut(s) 922, 1500, 1588
VpaK11BI GGWCC 1 cut(s) 1358
VspI ATTAAT 2 cut(s) 9, 396
XapI RAATTY 4 cut(s) 167, 554, 1095, 1121
XceI RCATGY 3 cut(s) 45, 432, 1323
XmiI GTMKAC 7 cut(s) 206, 300, 593, 942, 1050, 1258, 1617
XmnI GAANNNNTTC 2 cut(s) 478, 1095
XspI CTAG 6 cut(s) 179, 548, 956, 1224, 1638, 1649
Zsp2I ATGCAT 1 cut(s) 1248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.