RchiOBHm_Chr2g0139951

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001058
Physical Location & Seq
Forward (+)
57358281 .. 57360197
1917 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51043

Sequence Viewer

Length: 1695 bp
ATGATTCCAGCAGGGCTATATGAAGCAATTGCAAGAAATGACACTCAGACTTTCAAGGACATTGTTCGATACAATGGAGGATCTCTCTTGCAGAAAACTGAAGAAACAAGAGACTCAGTGTTACACCTTGCTGCCAGGTTCGGGCATGTTGAGCTGGTTACCTATATCATCACTCTGCATCCTGACTTTGTTTCTGTTAGGAACGTTAAGGAAGAAACTCCGTTACACGAAGCTTGTCGCCACTGGAATACCAATGTTGTGAAGCTGCTCTTAGAGGCCAATCCATATGTGGTTGGTGCTCTTAACCATGAAATGCAAACTGCTTTTTACATAGCCTGCAGTTGTGGGAATGTTGATGTTGTCAAGTACATGGTAAACCAACCAGGGGTGTTAGACTTTGAACAACATGAAGCTTCCAGTCCTCTTCATCATTCTATAGCAGAAGGCTATGATGTCGATATTGTCAAGACTATTTTGGAAGCATGTCCCCAGTTTGCTTGGAAGTTTGACAACAATGGGTATTTGCCGCTGCATTTGGCTTGCGAGAAAGGTCAGGTAGAGATAGTAAAAACACTCTTGAGGTTCAATCCAAACCTTTCTCTTGAACACAATCGCTACCGTTACACACCACTGCACGTGGCTGCAATGAATGGTCAAATCCAAATCCTTGAGGAACTTCTTTTGTGGTCTCCCGGATCCTTGAAAGTTTGCACAGGAAACGGAGAAAATGTGTTTCATCTTTCTGTAAGATTCAACAGATATGATGTGTTCACCTCTTTGGCAAAGAATTATAACCGCACCAGCCTGCTCAACCAGGCTGACAAGCAGGGTAACACAGTCTTACATCTTGCTGTCTCTGCAGGGAACACTAGATTATCAGAGTACATCATCAGCAATACTGCAGTAGATGTCAATTGTAGGAACCAAAGCGGTCAAACAGCATTGGACATCCTAAACGAGCAAAGCAGTTCGGCTGATCTTAGACATACGTTGAAGAGATTTGGTGGCAGAACAAGTATTGAGTTGTTCTCTTTAACCCCAAAGGTTAATGAGATATATTCTGGGACTACAACAACATCAGAATGTCCTAGAGCTGCACTGGAGAAAGAATTTGATAGGGTTTTATGTAACACGCGTCCAGGAAGTGATCAATCTGATTGTGAAATTGGCATGGGCCGTATGGCTCGAAACAATCAACAAATGGAAAACTTTGCTACAGTACAAGCAGAGATGAATCCCAGTCATGAAGTTGTGATAAATTCTGATATTCCTTTTAGCTCAGAATCGTATAGAACAAGTATGAGCTCCCCAGTCTTCAGTCCACAGAAGCTTCGAAAGCTAGGAGGGAAAGTGGGTAAAAGTAAGCAATATGAGATATATAGAGAGGCATTGCAAAATACTAGGAACACTATCACAATAGTTGCTGTTTTGATTGCCTCAGTTACTTTTACTGCTGGCATAAACCCACCTGGAGGTGTCTACCAGGAGGGATGGCTTAAAGGACAGTCAGTAGTTGGCAGAACAACAGCTTTTAAGGTCTTCACAATAAGTAACACAGCGGCATTGTTCATATCTATGTGCATTGTTATTTTATTGGTGAGCATCATTCCCTTCCGGCGAAAATCACTGATGAAACTACTTGTGGTTGCTCACAAAGTCCTCCTCATGGATAATCTTGCCACATGGACGTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

564

Amino Acids

63.04

Weight (kDa)

7.61

Isoelectric Point (pI)

33.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 6 - 94 2.5e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 103 - 160 8.9e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 154 - 227 5.8e-12 Ankyrin repeats (3 copies)
Ank_2 PF12796 246 - 316 7.3e-06 Ankyrin repeats (3 copies)
Ank_5 PF13857 269 - 317 1.7e-06 Ankyrin repeats (many copies)
PGG PF13962 462 - 560 1e-25 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 792
AccI GTMKAC 1 cut(s) 1479
AccII CGCG 1 cut(s) 1135
AciI CCGC 4 cut(s) 527, 796, 930, 1559
AclI AACGTT 1 cut(s) 204
AclWI GGATC 3 cut(s) 88, 690, 703
AcsI RAATTY 2 cut(s) 1109, 1258
AcuI CTGAAG 2 cut(s) 120, 1300
AcvI CACGTG 1 cut(s) 637
AfaI GTAC 3 cut(s) 368, 884, 1221
AfiI CCNNNNNNNGG 4 cut(s) 141, 385, 1472, 1666
AflIII ACRYGT 1 cut(s) 1133
AgsI TTSAA 7 cut(s) 55, 401, 586, 605, 703, 754, 994
AjiI CACGTC 1 cut(s) 1689
AjnI CCWGG 6 cut(s) 134, 382, 813, 1138, 1468, 1482
Alw21I GWGCWC 2 cut(s) 301, 1307
Alw26I GTCTC 3 cut(s) 105, 693, 859
AlwI GGATC 3 cut(s) 88, 690, 703
AoxI GGCC 2 cut(s) 276, 1174
ApeKI GCWGC 5 cut(s) 131, 265, 529, 641, 1094
ApoI RAATTY 2 cut(s) 1109, 1258
AspS9I GGNCC 1 cut(s) 1174
AsuC2I CCSGG 1 cut(s) 693
AsuHPI GGTGA 2 cut(s) 763, 1609
AsuII TTCGAA 1 cut(s) 1333
BamHI GGATCC 1 cut(s) 695
BanII GRGCYC 1 cut(s) 1307
BbrPI CACGTG 1 cut(s) 637
BbsI GAAGAC 2 cut(s) 1306, 1531
Bbv12I GWGCWC 2 cut(s) 301, 1307
BbvI GCAGC 5 cut(s) 118, 252, 516, 628, 1081
BccI CCATC 1 cut(s) 1485
BceAI ACGGC 1 cut(s) 1161
BciT130I CCWGG 6 cut(s) 136, 384, 815, 1140, 1470, 1484
BclI TGATCA 1 cut(s) 1147
BcnI CCSGG 1 cut(s) 693
BcoDI GTCTC 3 cut(s) 105, 693, 859
BfaI CTAG 4 cut(s) 870, 1089, 1340, 1401
BfmI CTRYAG 5 cut(s) 337, 435, 858, 900, 1215
BisI GCNGC 7 cut(s) 132, 266, 527, 530, 642, 1095, 1560
BlsI GCNGC 7 cut(s) 133, 267, 528, 531, 643, 1096, 1561
Bme1390I CCNGG 7 cut(s) 136, 384, 693, 815, 1140, 1470, 1484
BmgBI CACGTC 1 cut(s) 1689
BmgT120I GGNCC 1 cut(s) 1174
BmiI GGNNCC 2 cut(s) 697, 923
BmrFI CCNGG 7 cut(s) 136, 384, 693, 815, 1140, 1470, 1484
BmrI ACTGGG 3 cut(s) 484, 1233, 1304
BmsI GCATC 2 cut(s) 187, 1611
BmuI ACTGGG 3 cut(s) 484, 1233, 1304
BpiI GAAGAC 2 cut(s) 1306, 1531
BplI GAGNNNNNCTC 4 cut(s) 69, 101, 1013, 1045
BpmI CTGGAG 2 cut(s) 1121, 1491
Bpu14I TTCGAA 1 cut(s) 1333
BpuEI CTTGAG 2 cut(s) 598, 689
BpuMI CCSGG 1 cut(s) 693
BsaAI YACGTR 1 cut(s) 637
BsaI GGTCTC 1 cut(s) 693
BsaJI CCNNGG 1 cut(s) 383
BsaXI ACNNNNNCTCC 4 cut(s) 714, 744, 1335, 1365
Bsc4I CCNNNNNNNGG 4 cut(s) 141, 385, 1472, 1666
Bse1I ACTGG 6 cut(s) 248, 417, 490, 1104, 1239, 1310
Bse3DI GCAATG 2 cut(s) 651, 1388
BseBI CCWGG 6 cut(s) 136, 384, 815, 1140, 1470, 1484
BseDI CCNNGG 1 cut(s) 383
BseGI GGATG 3 cut(s) 178, 948, 1496
BseLI CCNNNNNNNGG 4 cut(s) 141, 385, 1472, 1666
BseMI GCAATG 2 cut(s) 651, 1388
BseMII CTCAG 4 cut(s) 59, 129, 1293, 1452
BseNI ACTGG 6 cut(s) 248, 417, 490, 1104, 1239, 1310
BseRI GAGGAG 1 cut(s) 1652
BseXI GCAGC 5 cut(s) 118, 252, 516, 628, 1081
BsgI GTGCAG 2 cut(s) 617, 1080
Bsh1236I CGCG 1 cut(s) 1135
BshFI GGCC 2 cut(s) 278, 1176
BsiHKAI GWGCWC 2 cut(s) 301, 1307
BsiSI CCGG 2 cut(s) 693, 1615
BslFI GGGAC 2 cut(s) 471, 1078
BslI CCNNNNNNNGG 4 cut(s) 141, 385, 1472, 1666
BsmAI GTCTC 3 cut(s) 105, 693, 859
BsmFI GGGAC 2 cut(s) 471, 1078
BsnI GGCC 2 cut(s) 278, 1176
Bso31I GGTCTC 1 cut(s) 693
Bsp119I TTCGAA 1 cut(s) 1333
Bsp1286I GDGCHC 2 cut(s) 301, 1307
Bsp143I GATC 4 cut(s) 80, 695, 976, 1147
BspACI CCGC 4 cut(s) 527, 796, 930, 1559
BspANI GGCC 2 cut(s) 278, 1176
BspCNI CTCAG 4 cut(s) 58, 128, 1292, 1451
BspFNI CGCG 1 cut(s) 1135
BspHI TCATGA 1 cut(s) 1243
BspLI GGNNCC 2 cut(s) 697, 923
BspMAI CTGCAG 3 cut(s) 341, 862, 904
BspPI GGATC 3 cut(s) 88, 690, 703
BspT104I TTCGAA 1 cut(s) 1333
BspTNI GGTCTC 1 cut(s) 693
BsrDI GCAATG 2 cut(s) 651, 1388
BsrI ACTGG 6 cut(s) 248, 417, 490, 1104, 1239, 1310
BssECI CCNNGG 1 cut(s) 383
BssMI GATC 4 cut(s) 80, 695, 976, 1147
Bst2UI CCWGG 6 cut(s) 136, 384, 815, 1140, 1470, 1484
Bst4CI ACNGT 4 cut(s) 620, 838, 1219, 1506
Bst6I CTCTTC 2 cut(s) 429, 989
BstBAI YACGTR 1 cut(s) 637
BstBI TTCGAA 1 cut(s) 1333
BstC8I GCNNGC 4 cut(s) 337, 541, 806, 1456
BstDEI CTNAG 6 cut(s) 45, 115, 271, 980, 1279, 1438
BstEII GGTNACC 1 cut(s) 157
BstF5I GGATG 3 cut(s) 178, 948, 1496
BstFNI CGCG 1 cut(s) 1135
BstKTI GATC 4 cut(s) 83, 698, 979, 1150
BstMAI GTCTC 3 cut(s) 105, 693, 859
BstMBI GATC 4 cut(s) 80, 695, 976, 1147
BstMWI GCNNNNNNNGC 3 cut(s) 151, 857, 1336
BstNI CCWGG 6 cut(s) 136, 384, 815, 1140, 1470, 1484
BstNSI RCATGY 2 cut(s) 149, 486
BstPI GGTNACC 1 cut(s) 157
BstSCI CCNGG 7 cut(s) 134, 382, 691, 813, 1138, 1468, 1482
BstSFI CTRYAG 5 cut(s) 337, 435, 858, 900, 1215
BstUI CGCG 1 cut(s) 1135
BstV1I GCAGC 5 cut(s) 118, 252, 516, 628, 1081
BstV2I GAAGAC 2 cut(s) 1306, 1531
BstX2I RGATCY 2 cut(s) 80, 695
BstYI RGATCY 2 cut(s) 80, 695
BsuRI GGCC 2 cut(s) 278, 1176
BtrI CACGTC 1 cut(s) 1689
BtsCI GGATG 3 cut(s) 178, 948, 1496
BtsI GCAGTG 1 cut(s) 629
BtsIMutI CAGTG 5 cut(s) 123, 241, 629, 1097, 1625
Cac8I GCNNGC 4 cut(s) 337, 541, 806, 1456
CciI TCATGA 1 cut(s) 1243
Cfr13I GGNCC 1 cut(s) 1174
CseI GACGC 1 cut(s) 1124
Csp6I GTAC 3 cut(s) 367, 883, 1220
CviAII CATG 9 cut(s) 146, 308, 370, 407, 483, 1171, 1244, 1666, 1683
CviQI GTAC 3 cut(s) 367, 883, 1220
DdeI CTNAG 6 cut(s) 45, 115, 271, 980, 1279, 1438
DpnI GATC 4 cut(s) 82, 697, 978, 1149
DpnII GATC 4 cut(s) 80, 695, 976, 1147
Eam1104I CTCTTC 2 cut(s) 429, 989
EarI CTCTTC 2 cut(s) 429, 989
Ecl136II GAGCTC 1 cut(s) 1305
Eco24I GRGCYC 1 cut(s) 1307
Eco31I GGTCTC 1 cut(s) 693
Eco53kI GAGCTC 1 cut(s) 1305
Eco57I CTGAAG 2 cut(s) 120, 1300
Eco72I CACGTG 1 cut(s) 637
Eco91I GGTNACC 1 cut(s) 157
EcoICRI GAGCTC 1 cut(s) 1305
EcoO65I GGTNACC 1 cut(s) 157
EcoRII CCWGG 6 cut(s) 134, 382, 813, 1138, 1468, 1482
EcoT38I GRGCYC 1 cut(s) 1307
FaeI CATG 9 cut(s) 149, 311, 373, 410, 486, 1174, 1247, 1669, 1686
FalI AAGNNNNNCTT 2 cut(s) 254, 286
FaqI GGGAC 2 cut(s) 471, 1078
FatI CATG 9 cut(s) 145, 307, 369, 406, 482, 1170, 1243, 1665, 1682
FauNDI CATATG 1 cut(s) 286
FbaI TGATCA 1 cut(s) 1147
FblI GTMKAC 1 cut(s) 1479
Fnu4HI GCNGC 7 cut(s) 132, 266, 527, 530, 642, 1095, 1560
FokI GGATG 3 cut(s) 165, 935, 1503
FriOI GRGCYC 1 cut(s) 1307
Fsp4HI GCNGC 7 cut(s) 132, 266, 527, 530, 642, 1095, 1560
FspBI CTAG 4 cut(s) 870, 1089, 1340, 1401
GluI GCNGC 7 cut(s) 132, 266, 527, 530, 642, 1095, 1560
GsuI CTGGAG 2 cut(s) 1121, 1491
HaeIII GGCC 2 cut(s) 278, 1176
HapII CCGG 2 cut(s) 693, 1615
HgaI GACGC 1 cut(s) 1124
Hin1II CATG 9 cut(s) 149, 311, 373, 410, 486, 1174, 1247, 1669, 1686
HindIII AAGCTT 3 cut(s) 231, 411, 1328
HinfI GANTC 5 cut(s) 4, 113, 750, 1234, 1283
HpaII CCGG 2 cut(s) 693, 1615
HphI GGTGA 2 cut(s) 763, 1609
Hpy166II GTNNAC 4 cut(s) 376, 771, 1322, 1480
Hpy188I TCNGA 6 cut(s) 48, 880, 1081, 1156, 1264, 1282
Hpy188III TCNNGA 5 cut(s) 182, 466, 577, 602, 1244
Hpy8I GTNNAC 4 cut(s) 376, 771, 1322, 1480
HpyAV CCTTC 2 cut(s) 437, 1621
HpyCH4III ACNGT 4 cut(s) 620, 838, 1219, 1506
HpyCH4IV ACGT 4 cut(s) 204, 636, 989, 1688
HpyF10VI GCNNNNNNNGC 3 cut(s) 151, 857, 1336
HpyF3I CTNAG 6 cut(s) 45, 115, 271, 980, 1279, 1438
HpySE526I ACGT 4 cut(s) 204, 636, 989, 1688
Hsp92II CATG 9 cut(s) 149, 311, 373, 410, 486, 1174, 1247, 1669, 1686
Ksp22I TGATCA 1 cut(s) 1147
Kzo9I GATC 4 cut(s) 80, 695, 976, 1147
LmnI GCTCC 1 cut(s) 1310
Lsp1109I GCAGC 5 cut(s) 118, 252, 516, 628, 1081
LweI GCATC 2 cut(s) 187, 1611
MaeI CTAG 4 cut(s) 870, 1089, 1340, 1401
MaeII ACGT 4 cut(s) 204, 636, 989, 1688
MaeIII GTNAC 8 cut(s) 120, 157, 222, 620, 830, 1127, 1441, 1550
MalI GATC 4 cut(s) 82, 697, 978, 1149
MboI GATC 4 cut(s) 80, 695, 976, 1147
MboII GAAGA 6 cut(s) 113, 224, 416, 1006, 1306, 1531
MfeI CAATTG 2 cut(s) 27, 913
MflI RGATCY 2 cut(s) 80, 695
MhlI GDGCHC 2 cut(s) 301, 1307
MluCI AATT 6 cut(s) 27, 787, 913, 1109, 1164, 1258
MluI ACGCGT 1 cut(s) 1133
MlyI GAGTC 1 cut(s) 107
MseI TTAA 6 cut(s) 207, 303, 1034, 1047, 1497, 1533
MslI CAYNNNNRTG 3 cut(s) 1081, 1574, 1687
MspA1I CMGCKG 2 cut(s) 529, 1559
MspI CCGG 2 cut(s) 693, 1615
MspR9I CCNGG 7 cut(s) 136, 384, 693, 815, 1140, 1470, 1484
MunI CAATTG 2 cut(s) 27, 913
MvaI CCWGG 6 cut(s) 136, 384, 815, 1140, 1470, 1484
MvnI CGCG 1 cut(s) 1135
MwoI GCNNNNNNNGC 3 cut(s) 151, 857, 1336
NciI CCSGG 1 cut(s) 693
NdeI CATATG 1 cut(s) 286
NdeII GATC 4 cut(s) 80, 695, 976, 1147
NlaIII CATG 9 cut(s) 149, 311, 373, 410, 486, 1174, 1247, 1669, 1686
NlaIV GGNNCC 2 cut(s) 697, 923
NspI RCATGY 2 cut(s) 149, 486
NspV TTCGAA 1 cut(s) 1333
PagI TCATGA 1 cut(s) 1243
PfeI GAWTC 4 cut(s) 4, 750, 1234, 1283
PfoI TCCNGGA 2 cut(s) 691, 1138
PkrI GCNGC 7 cut(s) 133, 267, 528, 531, 643, 1096, 1561
PleI GAGTC 1 cut(s) 107
PmaCI CACGTG 1 cut(s) 637
PmlI CACGTG 1 cut(s) 637
PpsI GAGTC 1 cut(s) 107
Ppu21I YACGTR 1 cut(s) 637
PsiI TTATAA 1 cut(s) 792
Psp124BI GAGCTC 1 cut(s) 1307
Psp1406I AACGTT 1 cut(s) 204
Psp6I CCWGG 6 cut(s) 134, 382, 813, 1138, 1468, 1482
PspCI CACGTG 1 cut(s) 637
PspEI GGTNACC 1 cut(s) 157
PspGI CCWGG 6 cut(s) 134, 382, 813, 1138, 1468, 1482
PspN4I GGNNCC 2 cut(s) 697, 923
PspPI GGNCC 1 cut(s) 1174
PstI CTGCAG 3 cut(s) 341, 862, 904
PsuI RGATCY 2 cut(s) 80, 695
RsaI GTAC 3 cut(s) 368, 884, 1221
RsaNI GTAC 3 cut(s) 367, 883, 1220
RseI CAYNNNNRTG 3 cut(s) 1081, 1574, 1687
SacI GAGCTC 1 cut(s) 1307
SaqAI TTAA 6 cut(s) 207, 303, 1034, 1047, 1497, 1533
SatI GCNGC 7 cut(s) 132, 266, 527, 530, 642, 1095, 1560
Sau3AI GATC 4 cut(s) 80, 695, 976, 1147
Sau96I GGNCC 1 cut(s) 1174
SchI GAGTC 1 cut(s) 107
ScrFI CCNGG 7 cut(s) 136, 384, 693, 815, 1140, 1470, 1484
SduI GDGCHC 2 cut(s) 301, 1307
SfaNI GCATC 2 cut(s) 187, 1611
SfcI CTRYAG 5 cut(s) 337, 435, 858, 900, 1215
SfuI TTCGAA 1 cut(s) 1333
SmiMI CAYNNNNRTG 3 cut(s) 1081, 1574, 1687
SmlI CTYRAG 2 cut(s) 577, 668
SmoI CTYRAG 2 cut(s) 577, 668
Sse9I AATT 6 cut(s) 27, 787, 913, 1109, 1164, 1258
SsiI CCGC 4 cut(s) 527, 796, 930, 1559
SspMI CTAG 4 cut(s) 870, 1089, 1340, 1401
SstI GAGCTC 1 cut(s) 1307
StyD4I CCNGG 7 cut(s) 134, 382, 691, 813, 1138, 1468, 1482
TaaI ACNGT 4 cut(s) 620, 838, 1219, 1506
TaiI ACGT 4 cut(s) 207, 639, 992, 1691
TaqI TCGA 4 cut(s) 67, 456, 1186, 1333
TasI AATT 6 cut(s) 27, 787, 913, 1109, 1164, 1258
TatI WGTACW 3 cut(s) 366, 882, 1219
TauI GCSGC 2 cut(s) 529, 1562
TfiI GAWTC 4 cut(s) 4, 750, 1234, 1283
Tru1I TTAA 6 cut(s) 207, 303, 1034, 1047, 1497, 1533
Tru9I TTAA 6 cut(s) 207, 303, 1034, 1047, 1497, 1533
TscAI CASTG 5 cut(s) 123, 248, 636, 1104, 1632
TseI GCWGC 5 cut(s) 131, 265, 529, 641, 1094
TspGWI ACGGA 2 cut(s) 210, 735
TspRI CASTG 5 cut(s) 123, 248, 636, 1104, 1632
XapI RAATTY 2 cut(s) 1109, 1258
XceI RCATGY 2 cut(s) 149, 486
XcmI CCANNNNNNNNNTGG 1 cut(s) 286
XmiI GTMKAC 1 cut(s) 1479
XspI CTAG 4 cut(s) 870, 1089, 1340, 1401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.