RchiOBHm_Chr2g0140011

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001058
Physical Location & Seq
Forward (+)
57384450 .. 57386266
1817 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51049

Sequence Viewer

Length: 1608 bp
ATGATTCCAGCTCTGTTTGAAGCAATTGCAAGAAATGACACACGGACTTTCAAGGACTTGGTCCGATACAATGGAGGATCTCTCTTGCAGAAAACTGAGGAAACAGGAAACTCAGTGTTACACCTTGCTGCAAGGTTCGGGCATGTTGAGCTGGTTGCCTATATCATTAAGCTGCTTCCTGACTTAGTTTCTGTTAGGAACGTTAAGGGAGAAACTCCATTACACGAAGCTTGTCGCCAAGGGAATACCAGTGTTGTGAAGCTGCTGTTAGAGGCCAGTCCATTCGTGACTTGTAGTCTTAACCATGAGCAAACTGCTTTCTTCATAGCTTGCAGTTATGGGAATGTTGAGGTTGTCAAGTACATGGCAAACCAACCAGGGGTCTTAGACTTTGAACAACATGAAGCTTTCAGTCCTCTTCATTTTTCTATAGCAGAAGGCTATGTCGATATTGTTAAGACTATTTTGGAAGCATGTCCCCGGTTTGCTTGGAAGGTTGACAACAAAGGGTATTTGCCGCTGCATTTGGCTTGTGAGAAAGGTCAGTTAGAGATAACAAAAACACTCTTGATGATCGATCCAAACCTTTCTCTTGAACTCAATCGCTACCGTTACACACCACTACACTTGGCTGCAATGAATGGTCAAAACCAAATCCTTGAAGAACTTCTTTTGTGCTCTCCCAGATCCCTAGAACTTCGCACAGGAGACGGAGAAACTGTGTTTCATCTTTCTGTAAGATTCAACAGATATGATGTGTTTACGTCTTTGGCAAAAAATTGTAACCACACCAACCTGCTCAACCAGGCTGACAAGCAGGGTAACACAGTCCTGCATCTTGCTCTCTCTGCAGGGAACACTAGGTTATCAGAGTACATCATCCGCAATACTGCAGTAGATGTCAATTGTAGGAACCAAAGAGGTCAAACAGCATTGGACATCCTAGACGAGCAAAGAATATCGTCTGATCTTAGACATACGTTGAAGAGTTTCGGTGGCAGAACAAGTACTAAGCTGTTCTCTTTAACCCCAAAGGTTGATGAGATTGATTCTGGGACTACAACAACATTGGAAAGTCCTAGAGCTGCACTGGAGCAAGAATTTGAAAGGCTTTTTGAATGTAACACGCGTCCAGGAAGTGATCAATCTGATTGTGAAATTGGCATGGGGCGTATGGCTCGGAACAATCAACAAATGGAAAACTTCGCTACAGTACAAGCAAAGATGAATCCCAGTCATGATGTTTTAATGAATTCTGATTTTCTTTTTAGCTCAGAATCATATAGAACAAGTATGAGCTCCCCAGTCTTGAATCCACAGCTGCTTCAAAAGCCAAGAGGGAAAGTGGATAAAACTAGGCAATATGAGATATATAGAGAGGCATTGCAAAATACTAGGAACACTATCACAATAGTTGCTGTTTTGATTGCCTCAGTTACTTTTACTGCTGGCATAAACCCACCTGGTGGTGTCTACCAGGAGGGATGGCTTAAAGGACAGTCAACAGTTGGCAGAACAACAGCTTTTAAGGTCTTCACAATAAGCAACACAGCGGCATTGTTCATATCTATGTGCATTGTATCATTCCCTTCCGGCGAAAATCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

535

Amino Acids

59.73

Weight (kDa)

6.55

Isoelectric Point (pI)

29.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 5 - 92 2.6e-13 Ankyrin repeats (3 copies)
Ank_4 PF13637 40 - 90 3.3e-09 Ankyrin repeats (many copies)
Ank PF00023 70 - 94 3.2e-06 Ankyrin repeat
Ank_4 PF13637 74 - 122 8.5e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 102 - 157 1.9e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 135 - 195 4e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 166 - 224 1.8e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 174 - 262 2.9e-11 Ankyrin repeats (3 copies)
Ank_2 PF12796 242 - 313 8.7e-06 Ankyrin repeats (3 copies)
Ank_5 PF13857 265 - 314 7.9e-06 Ankyrin repeats (many copies)
PGG PF13962 460 - 528 6.9e-21 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 804
AccB7I CCANNNNNTGG 1 cut(s) 1466
AccI GTMKAC 1 cut(s) 1473
AccII CGCG 1 cut(s) 1129
AciI CCGC 3 cut(s) 518, 883, 1553
AclI AACGTT 1 cut(s) 201
AclWI GGATC 3 cut(s) 85, 572, 681
AcsI RAATTY 2 cut(s) 1100, 1252
AdeI CACNNNGTG 1 cut(s) 1466
AfaI GTAC 4 cut(s) 362, 875, 1009, 1215
AfiI CCNNNNNNNGG 2 cut(s) 379, 1466
AflIII ACRYGT 1 cut(s) 1127
AhdI GACNNNNNGTC 1 cut(s) 294
AjnI CCWGG 5 cut(s) 376, 804, 1132, 1462, 1476
Alw21I GWGCWC 2 cut(s) 680, 1301
Alw26I GTCTC 1 cut(s) 702
AlwI GGATC 3 cut(s) 85, 572, 681
AoxI GGCC 1 cut(s) 273
ApeKI GCWGC 7 cut(s) 128, 172, 262, 520, 632, 1085, 1321
ApoI RAATTY 2 cut(s) 1100, 1252
Asp700I GAANNNNTTC 2 cut(s) 666, 989
AspS9I GGNCC 1 cut(s) 61
AsuC2I CCSGG 1 cut(s) 481
AvaII GGWCC 1 cut(s) 61
BanII GRGCYC 1 cut(s) 1301
BbsI GAAGAC 1 cut(s) 1525
Bbv12I GWGCWC 2 cut(s) 680, 1301
BbvI GCAGC 7 cut(s) 115, 159, 249, 507, 619, 1072, 1308
BccI CCATC 1 cut(s) 1479
BciT130I CCWGG 5 cut(s) 378, 806, 1134, 1464, 1478
BclI TGATCA 1 cut(s) 1141
BcnI CCSGG 1 cut(s) 481
BcoDI GTCTC 1 cut(s) 702
BfaI CTAG 6 cut(s) 692, 861, 944, 1080, 1356, 1395
BfmI CTRYAG 4 cut(s) 429, 849, 891, 1209
BfuAI ACCTGC 1 cut(s) 804
BisI GCNGC 9 cut(s) 129, 173, 263, 518, 521, 633, 1086, 1322, 1554
BlsI GCNGC 9 cut(s) 130, 174, 264, 519, 522, 634, 1087, 1323, 1555
BmcAI AGTACT 1 cut(s) 1009
Bme1390I CCNGG 6 cut(s) 378, 481, 806, 1134, 1464, 1478
Bme18I GGWCC 1 cut(s) 61
BmeRI GACNNNNNGTC 1 cut(s) 294
BmgT120I GGNCC 1 cut(s) 61
BmiI GGNNCC 1 cut(s) 914
BmrFI CCNGG 6 cut(s) 378, 481, 806, 1134, 1464, 1478
BmrI ACTGGG 2 cut(s) 1227, 1298
BmsI GCATC 1 cut(s) 844
BmuI ACTGGG 2 cut(s) 1227, 1298
BpiI GAAGAC 1 cut(s) 1525
BplI GAGNNNNNCTC 2 cut(s) 66, 98
BpmI CTGGAG 1 cut(s) 1112
BpuMI CCSGG 1 cut(s) 481
Bsa29I ATCGAT 1 cut(s) 576
BsaJI CCNNGG 3 cut(s) 238, 377, 479
BsaXI ACNNNNNCTCC 2 cut(s) 705, 735
Bsc4I CCNNNNNNNGG 2 cut(s) 379, 1466
Bse1I ACTGG 5 cut(s) 249, 276, 1095, 1233, 1304
Bse3DI GCAATG 2 cut(s) 642, 1382
BseBI CCWGG 5 cut(s) 378, 806, 1134, 1464, 1478
BseCI ATCGAT 1 cut(s) 576
BseDI CCNNGG 3 cut(s) 238, 377, 479
BseGI GGATG 3 cut(s) 879, 939, 1490
BseLI CCNNNNNNNGG 2 cut(s) 379, 1466
BseMI GCAATG 2 cut(s) 642, 1382
BseMII CTCAG 4 cut(s) 87, 126, 1287, 1446
BseNI ACTGG 5 cut(s) 249, 276, 1095, 1233, 1304
BseXI GCAGC 7 cut(s) 115, 159, 249, 507, 619, 1072, 1308
BsgI GTGCAG 1 cut(s) 1071
Bsh1236I CGCG 1 cut(s) 1129
BshFI GGCC 1 cut(s) 275
BshVI ATCGAT 1 cut(s) 576
BsiHKAI GWGCWC 2 cut(s) 680, 1301
BsiSI CCGG 2 cut(s) 481, 1593
BslFI GGGAC 2 cut(s) 462, 1069
BslI CCNNNNNNNGG 2 cut(s) 379, 1466
BsmAI GTCTC 1 cut(s) 702
BsmBI CGTCTC 1 cut(s) 702
BsmFI GGGAC 2 cut(s) 462, 1069
BsnI GGCC 1 cut(s) 275
Bsp1286I GDGCHC 2 cut(s) 680, 1301
Bsp143I GATC 6 cut(s) 77, 573, 577, 686, 967, 1141
BspACI CCGC 3 cut(s) 518, 883, 1553
BspANI GGCC 1 cut(s) 275
BspCNI CTCAG 4 cut(s) 88, 125, 1286, 1445
BspDI ATCGAT 1 cut(s) 576
BspFNI CGCG 1 cut(s) 1129
BspHI TCATGA 1 cut(s) 1237
BspLI GGNNCC 1 cut(s) 914
BspMAI CTGCAG 2 cut(s) 853, 895
BspMI ACCTGC 1 cut(s) 804
BspPI GGATC 3 cut(s) 85, 572, 681
BsrDI GCAATG 2 cut(s) 642, 1382
BsrI ACTGG 5 cut(s) 249, 276, 1095, 1233, 1304
BssECI CCNNGG 3 cut(s) 238, 377, 479
BssMI GATC 6 cut(s) 77, 573, 577, 686, 967, 1141
BssT1I CCWWGG 1 cut(s) 238
Bst2UI CCWGG 5 cut(s) 378, 806, 1134, 1464, 1478
Bst4CI ACNGT 6 cut(s) 611, 721, 829, 1213, 1500, 1507
Bst6I CTCTTC 2 cut(s) 423, 980
BstC8I GCNNGC 2 cut(s) 331, 1450
BstDEI CTNAG 8 cut(s) 96, 112, 184, 385, 971, 1011, 1273, 1432
BstF5I GGATG 3 cut(s) 879, 939, 1490
BstFNI CGCG 1 cut(s) 1129
BstKTI GATC 6 cut(s) 80, 576, 580, 689, 970, 1144
BstMAI GTCTC 1 cut(s) 702
BstMBI GATC 6 cut(s) 77, 573, 577, 686, 967, 1141
BstMWI GCNNNNNNNGC 3 cut(s) 148, 848, 1330
BstNI CCWGG 5 cut(s) 378, 806, 1134, 1464, 1478
BstNSI RCATGY 2 cut(s) 146, 477
BstSCI CCNGG 6 cut(s) 376, 479, 804, 1132, 1462, 1476
BstSFI CTRYAG 4 cut(s) 429, 849, 891, 1209
BstUI CGCG 1 cut(s) 1129
BstV1I GCAGC 7 cut(s) 115, 159, 249, 507, 619, 1072, 1308
BstV2I GAAGAC 1 cut(s) 1525
BstX2I RGATCY 2 cut(s) 77, 686
BstYI RGATCY 2 cut(s) 77, 686
Bsu15I ATCGAT 1 cut(s) 576
BsuRI GGCC 1 cut(s) 275
BsuTUI ATCGAT 1 cut(s) 576
BtsCI GGATG 3 cut(s) 879, 939, 1490
BtsIMutI CAGTG 4 cut(s) 120, 256, 1088, 1603
BveI ACCTGC 1 cut(s) 804
Cac8I GCNNGC 2 cut(s) 331, 1450
CciI TCATGA 1 cut(s) 1237
Cfr13I GGNCC 1 cut(s) 61
ClaI ATCGAT 1 cut(s) 576
CseI GACGC 1 cut(s) 1118
CsiI ACCWGGT 1 cut(s) 1462
Csp6I GTAC 4 cut(s) 361, 874, 1008, 1214
CspCI CAANNNNNGTGG 2 cut(s) 609, 644
CviAII CATG 7 cut(s) 143, 305, 364, 401, 474, 1165, 1238
CviQI GTAC 4 cut(s) 361, 874, 1008, 1214
DdeI CTNAG 8 cut(s) 96, 112, 184, 385, 971, 1011, 1273, 1432
DpnI GATC 6 cut(s) 79, 575, 579, 688, 969, 1143
DpnII GATC 6 cut(s) 77, 573, 577, 686, 967, 1141
DraIII CACNNNGTG 1 cut(s) 1466
DriI GACNNNNNGTC 1 cut(s) 294
Eam1104I CTCTTC 2 cut(s) 423, 980
Eam1105I GACNNNNNGTC 1 cut(s) 294
EarI CTCTTC 2 cut(s) 423, 980
Ecl136II GAGCTC 1 cut(s) 1299
Eco130I CCWWGG 1 cut(s) 238
Eco24I GRGCYC 1 cut(s) 1301
Eco47I GGWCC 1 cut(s) 61
Eco53kI GAGCTC 1 cut(s) 1299
EcoICRI GAGCTC 1 cut(s) 1299
EcoRI GAATTC 1 cut(s) 1252
EcoRII CCWGG 5 cut(s) 376, 804, 1132, 1462, 1476
EcoT14I CCWWGG 1 cut(s) 238
EcoT38I GRGCYC 1 cut(s) 1301
ErhI CCWWGG 1 cut(s) 238
Esp3I CGTCTC 1 cut(s) 702
FaeI CATG 7 cut(s) 146, 308, 367, 404, 477, 1168, 1241
FalI AAGNNNNNCTT 2 cut(s) 654, 686
FaqI GGGAC 2 cut(s) 462, 1069
FatI CATG 7 cut(s) 142, 304, 363, 400, 473, 1164, 1237
FbaI TGATCA 1 cut(s) 1141
FblI GTMKAC 1 cut(s) 1473
Fnu4HI GCNGC 9 cut(s) 129, 173, 263, 518, 521, 633, 1086, 1322, 1554
FokI GGATG 3 cut(s) 866, 926, 1497
FriOI GRGCYC 1 cut(s) 1301
Fsp4HI GCNGC 9 cut(s) 129, 173, 263, 518, 521, 633, 1086, 1322, 1554
FspBI CTAG 6 cut(s) 692, 861, 944, 1080, 1356, 1395
GluI GCNGC 9 cut(s) 129, 173, 263, 518, 521, 633, 1086, 1322, 1554
GsuI CTGGAG 1 cut(s) 1112
HaeIII GGCC 1 cut(s) 275
HapII CCGG 2 cut(s) 481, 1593
HgaI GACGC 1 cut(s) 1118
Hin1II CATG 7 cut(s) 146, 308, 367, 404, 477, 1168, 1241
HincII GTYRAC 2 cut(s) 499, 1503
HindII GTYRAC 2 cut(s) 499, 1503
HindIII AAGCTT 2 cut(s) 228, 405
HinfI GANTC 6 cut(s) 4, 741, 1049, 1228, 1277, 1312
HpaII CCGG 2 cut(s) 481, 1593
Hpy166II GTNNAC 4 cut(s) 499, 762, 1474, 1503
Hpy188I TCNGA 7 cut(s) 65, 871, 967, 1150, 1182, 1258, 1276
Hpy188III TCNNGA 6 cut(s) 179, 286, 568, 593, 1238, 1309
Hpy8I GTNNAC 4 cut(s) 499, 762, 1474, 1503
HpyAV CCTTC 3 cut(s) 431, 487, 1599
HpyCH4III ACNGT 6 cut(s) 611, 721, 829, 1213, 1500, 1507
HpyCH4IV ACGT 3 cut(s) 201, 764, 980
HpyF10VI GCNNNNNNNGC 3 cut(s) 148, 848, 1330
HpyF3I CTNAG 8 cut(s) 96, 112, 184, 385, 971, 1011, 1273, 1432
HpySE526I ACGT 3 cut(s) 201, 764, 980
Hsp92II CATG 7 cut(s) 146, 308, 367, 404, 477, 1168, 1241
Ksp22I TGATCA 1 cut(s) 1141
Kzo9I GATC 6 cut(s) 77, 573, 577, 686, 967, 1141
LmnI GCTCC 2 cut(s) 1093, 1304
Lsp1109I GCAGC 7 cut(s) 115, 159, 249, 507, 619, 1072, 1308
LweI GCATC 1 cut(s) 844
MabI ACCWGGT 1 cut(s) 1462
MaeI CTAG 6 cut(s) 692, 861, 944, 1080, 1356, 1395
MaeII ACGT 3 cut(s) 201, 764, 980
MaeIII GTNAC 7 cut(s) 117, 286, 611, 782, 821, 1121, 1435
MalI GATC 6 cut(s) 79, 575, 579, 688, 969, 1143
MboI GATC 6 cut(s) 77, 573, 577, 686, 967, 1141
MboII GAAGA 5 cut(s) 313, 410, 674, 997, 1525
MfeI CAATTG 2 cut(s) 24, 904
MflI RGATCY 2 cut(s) 77, 686
MhlI GDGCHC 2 cut(s) 680, 1301
MluCI AATT 6 cut(s) 24, 778, 904, 1100, 1158, 1252
MluI ACGCGT 1 cut(s) 1127
MroXI GAANNNNTTC 2 cut(s) 666, 989
MseI TTAA 8 cut(s) 168, 204, 300, 456, 1025, 1247, 1491, 1527
MslI CAYNNNNRTG 1 cut(s) 1568
MspA1I CMGCKG 3 cut(s) 520, 1321, 1553
MspI CCGG 2 cut(s) 481, 1593
MspR9I CCNGG 6 cut(s) 378, 481, 806, 1134, 1464, 1478
MunI CAATTG 2 cut(s) 24, 904
MvaI CCWGG 5 cut(s) 378, 806, 1134, 1464, 1478
MvnI CGCG 1 cut(s) 1129
MwoI GCNNNNNNNGC 3 cut(s) 148, 848, 1330
NciI CCSGG 1 cut(s) 481
NdeII GATC 6 cut(s) 77, 573, 577, 686, 967, 1141
NlaIII CATG 7 cut(s) 146, 308, 367, 404, 477, 1168, 1241
NlaIV GGNNCC 1 cut(s) 914
NmuCI GTSAC 1 cut(s) 286
NspI RCATGY 2 cut(s) 146, 477
PagI TCATGA 1 cut(s) 1237
PdmI GAANNNNTTC 2 cut(s) 666, 989
PfeI GAWTC 6 cut(s) 4, 741, 1049, 1228, 1277, 1312
PflFI GACNNNGTC 1 cut(s) 59
PflMI CCANNNNNTGG 1 cut(s) 1466
PfoI TCCNGGA 1 cut(s) 1132
PkrI GCNGC 9 cut(s) 130, 174, 264, 519, 522, 634, 1087, 1323, 1555
Psp124BI GAGCTC 1 cut(s) 1301
Psp1406I AACGTT 1 cut(s) 201
Psp6I CCWGG 5 cut(s) 376, 804, 1132, 1462, 1476
PspGI CCWGG 5 cut(s) 376, 804, 1132, 1462, 1476
PspN4I GGNNCC 1 cut(s) 914
PspPI GGNCC 1 cut(s) 61
PstI CTGCAG 2 cut(s) 853, 895
PsuI RGATCY 2 cut(s) 77, 686
PsyI GACNNNGTC 1 cut(s) 59
PvuII CAGCTG 1 cut(s) 1321
RsaI GTAC 4 cut(s) 362, 875, 1009, 1215
RsaNI GTAC 4 cut(s) 361, 874, 1008, 1214
RseI CAYNNNNRTG 1 cut(s) 1568
SacI GAGCTC 1 cut(s) 1301
SaqAI TTAA 8 cut(s) 168, 204, 300, 456, 1025, 1247, 1491, 1527
SatI GCNGC 9 cut(s) 129, 173, 263, 518, 521, 633, 1086, 1322, 1554
Sau3AI GATC 6 cut(s) 77, 573, 577, 686, 967, 1141
Sau96I GGNCC 1 cut(s) 61
ScaI AGTACT 1 cut(s) 1009
ScrFI CCNGG 6 cut(s) 378, 481, 806, 1134, 1464, 1478
SduI GDGCHC 2 cut(s) 680, 1301
SexAI ACCWGGT 1 cut(s) 1462
SfaNI GCATC 1 cut(s) 844
SfcI CTRYAG 4 cut(s) 429, 849, 891, 1209
SinI GGWCC 1 cut(s) 61
SmiMI CAYNNNNRTG 1 cut(s) 1568
Sse9I AATT 6 cut(s) 24, 778, 904, 1100, 1158, 1252
SsiI CCGC 3 cut(s) 518, 883, 1553
SspMI CTAG 6 cut(s) 692, 861, 944, 1080, 1356, 1395
SstI GAGCTC 1 cut(s) 1301
StyD4I CCNGG 6 cut(s) 376, 479, 804, 1132, 1462, 1476
StyI CCWWGG 1 cut(s) 238
TaaI ACNGT 6 cut(s) 611, 721, 829, 1213, 1500, 1507
TaiI ACGT 3 cut(s) 204, 767, 983
TaqI TCGA 2 cut(s) 447, 576
TasI AATT 6 cut(s) 24, 778, 904, 1100, 1158, 1252
TatI WGTACW 4 cut(s) 360, 873, 1007, 1213
TauI GCSGC 2 cut(s) 520, 1556
TfiI GAWTC 6 cut(s) 4, 741, 1049, 1228, 1277, 1312
Tru1I TTAA 8 cut(s) 168, 204, 300, 456, 1025, 1247, 1491, 1527
Tru9I TTAA 8 cut(s) 168, 204, 300, 456, 1025, 1247, 1491, 1527
TscAI CASTG 3 cut(s) 120, 256, 1095
TseFI GTSAC 1 cut(s) 286
TseI GCWGC 7 cut(s) 128, 172, 262, 520, 632, 1085, 1321
Tsp45I GTSAC 1 cut(s) 286
TspDTI ATGAA 8 cut(s) 313, 410, 417, 653, 716, 1241, 1265, 1552
TspGWI ACGGA 2 cut(s) 58, 726
TspRI CASTG 3 cut(s) 120, 256, 1095
Tth111I GACNNNGTC 1 cut(s) 59
Van91I CCANNNNNTGG 1 cut(s) 1466
VpaK11BI GGWCC 1 cut(s) 61
XapI RAATTY 2 cut(s) 1100, 1252
XceI RCATGY 2 cut(s) 146, 477
XmiI GTMKAC 1 cut(s) 1473
XmnI GAANNNNTTC 2 cut(s) 666, 989
XspI CTAG 6 cut(s) 692, 861, 944, 1080, 1356, 1395
ZrmI AGTACT 1 cut(s) 1009
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.