Rw2G033650

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001058
Physical Location & Seq
Forward (+)
55038904 .. 55040229
1326 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G033650.1

Sequence Viewer

Length: 861 bp
ATGATTGATCCAGACCTTGCTCTTGAATTTGACCATTATCGTTACACACCAGTACACTTGGCTGCAATGGTCAAATCAAAATCCGCTGACAAGCATGGTAACACAATCTTACATCTTGCTGTCTCTGCAGGGAACATTACATTGTCGGAGCACATAATCAGGAATACTACAGTTGATGTCAATTGTAGAAATCAAAGAGGTCAAATAGCAATGGACATCTTATGCCAGCAAAGAAGAGCGTCGGATGTCAGAAACATGTTGAAGAGTTTTGGTGGCAGGAAGAGCACTGAGCTGTTGTCTTTAACACCAAAGATTGATGAGATAGATTATGAGACTACAACAACATCAGAACGTCCTAGAGTTGTACTTGAGCAAGAATTTGAAATGCTTTTTGAAGGCATCACACATCCGGGAAGTGATCAATATGATTGTGAAATTGGTACTTTCACTACTGGTATAAATCCTCCTGGTGGTGTCTACCAGGAGGGCCAGCTTAAAGGACAGTCAACAGTTGGCAGAACAACAGCTTTTATGGTCTTTGCAATATGCAACACAGCAGCACTTTTCATATCTATGTGCATTGTCATTGTCTTGGTCAGCAGCATTCCCTTCTGGCGAAAACCACTAATGAAACTACTGGTGGTTGCTCACAAAGTAATGTGGGTGGCGGTGTCACTAATGGCCACAGCTTATGTCGCAGCCATGTGGATGATCATGCCACACGGTGAAGGTGTAAATTGGACCCTTGAAGTAGAGATATCTGTGTGTACAGGGACTATGGGATCTGTTTTTTTTATTCCTTGGTGTCACATGGACCAGACACTGGTTGATGAAGTTCAAGTGGAGGAAGCAGAGAGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

286

Amino Acids

31.95

Weight (kDa)

5.47

Isoelectric Point (pI)

25.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGG PF13962 148 - 237 1e-20 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 823
AccI GTMKAC 1 cut(s) 477
AciI CCGC 2 cut(s) 84, 668
AclWI GGATC 2 cut(s) 2, 790
AcoI YGGCCR 1 cut(s) 681
AcsI RAATTY 2 cut(s) 26, 377
AdeI CACNNNGTG 1 cut(s) 725
AfaI GTAC 4 cut(s) 54, 366, 442, 769
AfiI CCNNNNNNNGG 2 cut(s) 470, 823
AflIII ACRYGT 1 cut(s) 255
AgsI TTSAA 6 cut(s) 26, 262, 383, 395, 749, 839
AjnI CCWGG 2 cut(s) 466, 480
AluBI AGCT 4 cut(s) 292, 493, 527, 689
AluI AGCT 4 cut(s) 292, 493, 527, 689
Alw21I GWGCWC 2 cut(s) 153, 287
Alw26I GTCTC 2 cut(s) 127, 326
AlwI GGATC 2 cut(s) 2, 790
AlwNI CAGNNNCTG 1 cut(s) 823
AoxI GGCC 2 cut(s) 487, 681
ApeKI GCWGC 4 cut(s) 62, 557, 600, 698
ApoI RAATTY 2 cut(s) 26, 377
AspS9I GGNCC 3 cut(s) 487, 741, 814
AsuC2I CCSGG 1 cut(s) 411
AsuHPI GGTGA 1 cut(s) 737
AvaII GGWCC 2 cut(s) 741, 814
BalI TGGCCA 1 cut(s) 683
Bbv12I GWGCWC 2 cut(s) 153, 287
BbvI GCAGC 4 cut(s) 49, 569, 612, 710
BciT130I CCWGG 2 cut(s) 468, 482
BclI TGATCA 2 cut(s) 418, 711
BcnI CCSGG 1 cut(s) 411
BcoDI GTCTC 2 cut(s) 127, 326
BfaI CTAG 1 cut(s) 357
BfmI CTRYAG 2 cut(s) 126, 168
BisI GCNGC 4 cut(s) 63, 558, 601, 699
BlsI GCNGC 4 cut(s) 64, 559, 602, 700
Bme1390I CCNGG 3 cut(s) 411, 468, 482
Bme18I GGWCC 2 cut(s) 741, 814
BmgT120I GGNCC 3 cut(s) 487, 741, 814
BmiI GGNNCC 1 cut(s) 743
BmrFI CCNGG 3 cut(s) 411, 468, 482
BmsI GCATC 1 cut(s) 408
BpuEI CTTGAG 1 cut(s) 389
BpuMI CCSGG 1 cut(s) 411
BsaJI CCNNGG 1 cut(s) 800
Bsc4I CCNNNNNNNGG 2 cut(s) 470, 823
Bse1I ACTGG 4 cut(s) 50, 457, 642, 828
Bse3DI GCAATG 2 cut(s) 72, 216
BseBI CCWGG 2 cut(s) 468, 482
BseDI CCNNGG 1 cut(s) 800
BseGI GGATG 3 cut(s) 250, 406, 714
BseLI CCNNNNNNNGG 2 cut(s) 470, 823
BseMI GCAATG 2 cut(s) 72, 216
BseMII CTCAG 1 cut(s) 279
BseNI ACTGG 4 cut(s) 50, 457, 642, 828
BseXI GCAGC 4 cut(s) 49, 569, 612, 710
BshFI GGCC 2 cut(s) 489, 683
BsiHKAI GWGCWC 2 cut(s) 153, 287
BsiSI CCGG 1 cut(s) 410
BslFI GGGAC 1 cut(s) 787
BslI CCNNNNNNNGG 2 cut(s) 470, 823
BsmAI GTCTC 2 cut(s) 127, 326
BsmFI GGGAC 1 cut(s) 787
BsmI GAATGC 1 cut(s) 603
BsnI GGCC 2 cut(s) 489, 683
Bsp1286I GDGCHC 2 cut(s) 153, 287
Bsp1407I TGTACA 1 cut(s) 767
Bsp143I GATC 4 cut(s) 7, 418, 711, 782
BspACI CCGC 2 cut(s) 84, 668
BspANI GGCC 2 cut(s) 489, 683
BspCNI CTCAG 1 cut(s) 280
BspLI GGNNCC 1 cut(s) 743
BspMAI CTGCAG 1 cut(s) 130
BspPI GGATC 2 cut(s) 2, 790
BspQI GCTCTTC 2 cut(s) 229, 275
BsrDI GCAATG 2 cut(s) 72, 216
BsrGI TGTACA 1 cut(s) 767
BsrI ACTGG 4 cut(s) 50, 457, 642, 828
BssECI CCNNGG 1 cut(s) 800
BssMI GATC 4 cut(s) 7, 418, 711, 782
BssT1I CCWWGG 1 cut(s) 800
Bst2UI CCWGG 2 cut(s) 468, 482
Bst4CI ACNGT 4 cut(s) 172, 504, 511, 725
Bst6I CTCTTC 3 cut(s) 229, 257, 275
BstAUI TGTACA 1 cut(s) 767
BstC8I GCNNGC 2 cut(s) 227, 491
BstDEI CTNAG 1 cut(s) 288
BstF5I GGATG 3 cut(s) 250, 406, 714
BstKTI GATC 4 cut(s) 10, 421, 714, 785
BstMAI GTCTC 2 cut(s) 127, 326
BstMBI GATC 4 cut(s) 7, 418, 711, 782
BstMWI GCNNNNNNNGC 3 cut(s) 125, 282, 695
BstNI CCWGG 2 cut(s) 468, 482
BstNSI RCATGY 1 cut(s) 259
BstSCI CCNGG 3 cut(s) 409, 466, 480
BstSFI CTRYAG 2 cut(s) 126, 168
BstV1I GCAGC 4 cut(s) 49, 569, 612, 710
BstX2I RGATCY 1 cut(s) 782
BstYI RGATCY 1 cut(s) 782
BsuRI GGCC 2 cut(s) 489, 683
BtsCI GGATG 3 cut(s) 250, 406, 714
BtsIMutI CAGTG 2 cut(s) 285, 821
Cac8I GCNNGC 2 cut(s) 227, 491
CaiI CAGNNNCTG 1 cut(s) 823
Cfr13I GGNCC 3 cut(s) 487, 741, 814
CseI GACGC 1 cut(s) 228
Csp6I GTAC 4 cut(s) 53, 365, 441, 768
CviAII CATG 5 cut(s) 95, 256, 703, 715, 811
CviJI RGCY 8 cut(s) 62, 292, 489, 493, 527, 683, 689, 701
CviKI_1 RGCY 8 cut(s) 62, 292, 489, 493, 527, 683, 689, 701
CviQI GTAC 4 cut(s) 53, 365, 441, 768
DdeI CTNAG 1 cut(s) 288
DpnI GATC 4 cut(s) 9, 420, 713, 784
DpnII GATC 4 cut(s) 7, 418, 711, 782
DraIII CACNNNGTG 1 cut(s) 725
EaeI YGGCCR 1 cut(s) 681
Eam1104I CTCTTC 3 cut(s) 229, 257, 275
EarI CTCTTC 3 cut(s) 229, 257, 275
Eco130I CCWWGG 1 cut(s) 800
Eco32I GATATC 1 cut(s) 759
Eco47I GGWCC 2 cut(s) 741, 814
EcoRII CCWGG 2 cut(s) 466, 480
EcoRV GATATC 1 cut(s) 759
EcoT14I CCWWGG 1 cut(s) 800
ErhI CCWWGG 1 cut(s) 800
FaeI CATG 5 cut(s) 98, 259, 706, 718, 814
FaqI GGGAC 1 cut(s) 787
FatI CATG 5 cut(s) 94, 255, 702, 714, 810
FbaI TGATCA 2 cut(s) 418, 711
FblI GTMKAC 1 cut(s) 477
Fnu4HI GCNGC 4 cut(s) 63, 558, 601, 699
FokI GGATG 3 cut(s) 257, 393, 721
Fsp4HI GCNGC 4 cut(s) 63, 558, 601, 699
FspBI CTAG 1 cut(s) 357
GluI GCNGC 4 cut(s) 63, 558, 601, 699
HaeIII GGCC 2 cut(s) 489, 683
HapII CCGG 1 cut(s) 410
HgaI GACGC 1 cut(s) 228
Hin1II CATG 5 cut(s) 98, 259, 706, 718, 814
HincII GTYRAC 1 cut(s) 507
HindII GTYRAC 1 cut(s) 507
HpaII CCGG 1 cut(s) 410
HphI GGTGA 1 cut(s) 737
Hpy166II GTNNAC 4 cut(s) 55, 478, 507, 768
Hpy188I TCNGA 4 cut(s) 148, 244, 251, 349
Hpy188III TCNNGA 3 cut(s) 11, 23, 160
Hpy8I GTNNAC 4 cut(s) 55, 478, 507, 768
Hpy99I CGWCG 1 cut(s) 244
HpyAV CCTTC 3 cut(s) 389, 619, 722
HpyCH4III ACNGT 4 cut(s) 172, 504, 511, 725
HpyCH4IV ACGT 1 cut(s) 352
HpyCH4V TGCA 5 cut(s) 65, 128, 542, 549, 579
HpyF10VI GCNNNNNNNGC 3 cut(s) 125, 282, 695
HpyF3I CTNAG 1 cut(s) 288
HpySE526I ACGT 1 cut(s) 352
Hsp92II CATG 5 cut(s) 98, 259, 706, 718, 814
Ksp22I TGATCA 2 cut(s) 418, 711
Kzo9I GATC 4 cut(s) 7, 418, 711, 782
LguI GCTCTTC 2 cut(s) 229, 275
LmnI GCTCC 1 cut(s) 148
Lsp1109I GCAGC 4 cut(s) 49, 569, 612, 710
LweI GCATC 1 cut(s) 408
MaeI CTAG 1 cut(s) 357
MaeII ACGT 1 cut(s) 352
MaeIII GTNAC 4 cut(s) 41, 98, 672, 806
MalI GATC 4 cut(s) 9, 420, 713, 784
MboI GATC 4 cut(s) 7, 418, 711, 782
MboII GAAGA 3 cut(s) 246, 274, 292
MfeI CAATTG 1 cut(s) 181
MflI RGATCY 1 cut(s) 782
MhlI GDGCHC 2 cut(s) 153, 287
MlsI TGGCCA 1 cut(s) 683
MluCI AATT 5 cut(s) 26, 181, 377, 435, 736
MluNI TGGCCA 1 cut(s) 683
MmeI TCCRAC 2 cut(s) 126, 222
MnlI CCTC 5 cut(s) 191, 474, 478, 838, 849
Mox20I TGGCCA 1 cut(s) 683
MscI TGGCCA 1 cut(s) 683
MseI TTAA 2 cut(s) 302, 495
MslI CAYNNNNRTG 2 cut(s) 572, 707
Msp20I TGGCCA 1 cut(s) 683
MspA1I CMGCKG 1 cut(s) 86
MspI CCGG 1 cut(s) 410
MspR9I CCNGG 3 cut(s) 411, 468, 482
MunI CAATTG 1 cut(s) 181
Mva1269I GAATGC 1 cut(s) 603
MvaI CCWGG 2 cut(s) 468, 482
MwoI GCNNNNNNNGC 3 cut(s) 125, 282, 695
NciI CCSGG 1 cut(s) 411
NdeII GATC 4 cut(s) 7, 418, 711, 782
NlaIII CATG 5 cut(s) 98, 259, 706, 718, 814
NlaIV GGNNCC 1 cut(s) 743
NmuCI GTSAC 2 cut(s) 672, 806
NspI RCATGY 1 cut(s) 259
PciI ACATGT 1 cut(s) 255
PciSI GCTCTTC 2 cut(s) 229, 275
PctI GAATGC 1 cut(s) 603
PflMI CCANNNNNTGG 1 cut(s) 823
PfoI TCCNGGA 1 cut(s) 409
PkrI GCNGC 4 cut(s) 64, 559, 602, 700
PscI ACATGT 1 cut(s) 255
Psp6I CCWGG 2 cut(s) 466, 480
PspGI CCWGG 2 cut(s) 466, 480
PspN4I GGNNCC 1 cut(s) 743
PspPI GGNCC 3 cut(s) 487, 741, 814
PstI CTGCAG 1 cut(s) 130
PstNI CAGNNNCTG 1 cut(s) 823
PsuI RGATCY 1 cut(s) 782
RsaI GTAC 4 cut(s) 54, 366, 442, 769
RsaNI GTAC 4 cut(s) 53, 365, 441, 768
RseI CAYNNNNRTG 2 cut(s) 572, 707
SapI GCTCTTC 2 cut(s) 229, 275
SaqAI TTAA 2 cut(s) 302, 495
SatI GCNGC 4 cut(s) 63, 558, 601, 699
Sau3AI GATC 4 cut(s) 7, 418, 711, 782
Sau96I GGNCC 3 cut(s) 487, 741, 814
ScrFI CCNGG 3 cut(s) 411, 468, 482
SduI GDGCHC 2 cut(s) 153, 287
SetI ASST 9 cut(s) 18, 202, 294, 355, 495, 529, 691, 733, 860
SfaNI GCATC 1 cut(s) 408
SfcI CTRYAG 2 cut(s) 126, 168
SinI GGWCC 2 cut(s) 741, 814
SmiMI CAYNNNNRTG 2 cut(s) 572, 707
SmlI CTYRAG 1 cut(s) 368
SmoI CTYRAG 1 cut(s) 368
Sse9I AATT 5 cut(s) 26, 181, 377, 435, 736
SsiI CCGC 2 cut(s) 84, 668
SspMI CTAG 1 cut(s) 357
StyD4I CCNGG 3 cut(s) 409, 466, 480
StyI CCWWGG 1 cut(s) 800
TaaI ACNGT 4 cut(s) 172, 504, 511, 725
TaiI ACGT 1 cut(s) 355
TasI AATT 5 cut(s) 26, 181, 377, 435, 736
TatI WGTACW 3 cut(s) 52, 364, 767
Tru1I TTAA 2 cut(s) 302, 495
Tru9I TTAA 2 cut(s) 302, 495
TscAI CASTG 2 cut(s) 292, 828
TseFI GTSAC 2 cut(s) 672, 806
TseI GCWGC 4 cut(s) 62, 557, 600, 698
Tsp45I GTSAC 2 cut(s) 672, 806
TspDTI ATGAA 3 cut(s) 556, 644, 846
TspRI CASTG 2 cut(s) 292, 828
Van91I CCANNNNNTGG 1 cut(s) 823
VpaK11BI GGWCC 2 cut(s) 741, 814
XapI RAATTY 2 cut(s) 26, 377
XceI RCATGY 1 cut(s) 259
XmiI GTMKAC 1 cut(s) 477
XspI CTAG 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.