Rh2AG410600

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0001058
Physical Location & Seq
Forward (+)
60913471 .. 60917000
3530 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG410600.1

Sequence Viewer

Length: 1449 bp
ATGGACAAAATCGCAAAATACAAAATTAAACACCGGATTGCAAATAATGCAACACACGAGCATCAACTTGCAAGGATGTATAAATGGGGACAAAATTCCAGCGCCTCTGCTTTCGGATTCCCTGCGACGGGCGCCGATGCTCCACCAGAAGGCTATGTTGATATTGTTAGGACTATTTTGAAAGGTTGCCCCAGGTTTGCTTGGAAGGTTGACAGCAATGGGTGTCTACCACTGCATATAGCTTGCGAAAAAGGTCACTTAGAGATAGCCAGAACACTATTGATGATTGATCCAGACCTTGCTCTTGAATTTGACAATTACCGTTACACACCAGTACACTCGGCTGTAATGAATGGTCAAATCAAAATCCTTGAGGAACTCCTTTTGTGCTCTCCCAAATGTTTAGCAAGGCTCACAGGAGATGGAGAAACTGTCTTTCATCTTTCAGTAAGATTCAATAGATACGGTGTGTTTACCTCTTTGGTAAAGAATTATAACAGTACCTATCTCCTCAACCAGGCTGACAAGCTTGGTAACACAATCTTACATCTTGCTGTCTCTGCATGGAACATTATATTGTCGGAGCACATCATCAGGAATACTACAGTTGATGTCAATTGTAGAAATCAAAGAGGTGAAACAGCAATGGACATCTTAGGCCAGCAAAGAAGAATGTCGGATATAAGAAACATGTTGAAGAGTTTTGGTGGCAGCAAGAGCACTGAGCTGTTGTCTTTAACACCAAAGATTGATGAGATAGATAATGAGACTACAACAACATCAGAAAGTCCTAGAGTTGTACTTGAGCAAGAATTTGAAATACTTTTCGAAGGTATCACACATCCAGGAAGTGATCAATATGATTGTGAAATTGGTATGGGGCGTATGGCTAGAAATGATCAACCAATGGAAGATTTCACTGTAGTACAAGAAAAGATGAATCCAGGTCATCAAGTTGTGATGAACTCTGACAATCTTTTTGGCCTCGATTGTTTTGAAACAAGAATGAGCTCCACAGCCTTGAATGAGCAGCATCTTCCAAAGCCAAGAGGGCAAAACAAAGTGGGAAAAACTAGGCAATATGAGATATATAGAGAGGCACTGCAAAATACCATGGACACAATAACATTAGTTGCCATTCCAATTGCCCCAGGTACTTTCACTACTGGTATATACCCTCCTGGTGGTGTCTACGAGGAGGGCCAGCTTAAAGGACAGTCAACGGTTGGCAGAACAACAGCTTTTAAGATCTTTGCAATATGCAACACAGCAGCACTTTTCATATCTATGTGCATTGTCATTGTCTTGGTCAGCATCATTCATCATTCCCTTCCGGCGAAAACCACTAATGAAACTACTGGTGGTTGCTCACAAAGTAATGTGGGTGGCGGTGTCACTGATGGCCACAGCTTTTATTGCAGCCATGTGGATGATCATGCCACACGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

482

Amino Acids

53.5

Weight (kDa)

6.28

Isoelectric Point (pI)

30.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 51 - 128 2e-11 Ankyrin repeats (3 copies)
PGG PF13962 366 - 439 1.8e-17 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 495
AccB1I GGYRCC 1 cut(s) 131
AccI GTMKAC 2 cut(s) 226, 1191
AciI CCGC 1 cut(s) 1389
AclWI GGATC 1 cut(s) 284
AcoI YGGCCR 1 cut(s) 1402
AcsI RAATTY 3 cut(s) 94, 308, 812
AcyI GRCGYC 1 cut(s) 132
AfaI GTAC 5 cut(s) 336, 502, 801, 927, 1156
AfiI CCNNNNNNNGG 5 cut(s) 127, 128, 149, 517, 1184
AflIII ACRYGT 1 cut(s) 690
AgsI TTSAA 7 cut(s) 181, 308, 457, 697, 818, 998, 1024
AjnI CCWGG 6 cut(s) 191, 516, 844, 943, 1150, 1180
AluBI AGCT 7 cut(s) 242, 529, 727, 1011, 1207, 1241, 1410
AluI AGCT 7 cut(s) 242, 529, 727, 1011, 1207, 1241, 1410
Alw21I GWGCWC 4 cut(s) 392, 588, 722, 1013
Alw26I GTCTC 2 cut(s) 562, 761
AlwI GGATC 1 cut(s) 284
AoxI GGCC 4 cut(s) 658, 982, 1201, 1402
ApeKI GCWGC 4 cut(s) 711, 1030, 1271, 1419
ApoI RAATTY 3 cut(s) 94, 308, 812
ArsI GACNNNNNNTTYG 2 cut(s) 962, 994
AspLEI GCGC 2 cut(s) 104, 134
AspS9I GGNCC 1 cut(s) 1201
AsuHPI GGTGA 1 cut(s) 647
AsuII TTCGAA 1 cut(s) 828
BalI TGGCCA 1 cut(s) 1404
BanI GGYRCC 1 cut(s) 131
BanII GRGCYC 1 cut(s) 1013
BauI CACGAG 1 cut(s) 56
Bbv12I GWGCWC 4 cut(s) 392, 588, 722, 1013
BbvI GCAGC 4 cut(s) 723, 1042, 1283, 1431
BccI CCATC 2 cut(s) 416, 1394
BciT130I CCWGG 6 cut(s) 193, 518, 846, 945, 1152, 1182
BclI TGATCA 3 cut(s) 853, 898, 1432
BcoDI GTCTC 2 cut(s) 562, 761
BfaI CTAG 3 cut(s) 792, 891, 1074
BfmI CTRYAG 2 cut(s) 603, 921
BfoI RGCGCY 2 cut(s) 105, 135
BglI GCCNNNNNGGC 1 cut(s) 1051
BglII AGATCT 1 cut(s) 1248
BisI GCNGC 4 cut(s) 712, 1031, 1272, 1420
BlsI GCNGC 4 cut(s) 713, 1032, 1273, 1421
Bme1390I CCNGG 6 cut(s) 193, 518, 846, 945, 1152, 1182
BmgT120I GGNCC 1 cut(s) 1201
BmiI GGNNCC 1 cut(s) 133
BmrFI CCNGG 6 cut(s) 193, 518, 846, 945, 1152, 1182
BmsI GCATC 4 cut(s) 70, 127, 1042, 1323
Bpu14I TTCGAA 1 cut(s) 828
BpuEI CTTGAG 2 cut(s) 392, 824
BsaHI GRCGYC 1 cut(s) 132
BsaJI CCNNGG 3 cut(s) 191, 1113, 1150
BsaWI WCCGGW 1 cut(s) 33
BsaXI ACNNNNNCTCC 2 cut(s) 417, 447
Bsc4I CCNNNNNNNGG 5 cut(s) 127, 128, 149, 517, 1184
Bse1I ACTGG 3 cut(s) 332, 1171, 1363
Bse3DI GCAATG 2 cut(s) 223, 651
BseBI CCWGG 6 cut(s) 193, 518, 846, 945, 1152, 1182
BseDI CCNNGG 3 cut(s) 191, 1113, 1150
BseGI GGATG 3 cut(s) 81, 841, 1435
BseLI CCNNNNNNNGG 5 cut(s) 127, 128, 149, 517, 1184
BseMI GCAATG 2 cut(s) 223, 651
BseMII CTCAG 1 cut(s) 714
BseNI ACTGG 3 cut(s) 332, 1171, 1363
BseRI GAGGAG 2 cut(s) 500, 1211
BseXI GCAGC 4 cut(s) 723, 1042, 1283, 1431
BshFI GGCC 4 cut(s) 660, 984, 1203, 1404
BshNI GGYRCC 1 cut(s) 131
BsiHKAI GWGCWC 4 cut(s) 392, 588, 722, 1013
BsiSI CCGG 2 cut(s) 34, 1334
BslFI GGGAC 1 cut(s) 102
BslI CCNNNNNNNGG 5 cut(s) 127, 128, 149, 517, 1184
BsmAI GTCTC 2 cut(s) 562, 761
BsmFI GGGAC 1 cut(s) 102
BsnI GGCC 4 cut(s) 660, 984, 1203, 1404
Bsp119I TTCGAA 1 cut(s) 828
Bsp1286I GDGCHC 4 cut(s) 392, 588, 722, 1013
Bsp143I GATC 5 cut(s) 289, 853, 898, 1248, 1432
Bsp19I CCATGG 1 cut(s) 1113
BspACI CCGC 1 cut(s) 1389
BspANI GGCC 4 cut(s) 660, 984, 1203, 1404
BspCNI CTCAG 1 cut(s) 715
BspLI GGNNCC 1 cut(s) 133
BspPI GGATC 1 cut(s) 284
BspT104I TTCGAA 1 cut(s) 828
BspT107I GGYRCC 1 cut(s) 131
BsrDI GCAATG 2 cut(s) 223, 651
BsrI ACTGG 3 cut(s) 332, 1171, 1363
BssECI CCNNGG 3 cut(s) 191, 1113, 1150
BssMI GATC 5 cut(s) 289, 853, 898, 1248, 1432
BssNI GRCGYC 1 cut(s) 132
BssSI CACGAG 1 cut(s) 56
BssT1I CCWWGG 1 cut(s) 1113
Bst2BI CACGAG 1 cut(s) 56
Bst2UI CCWGG 6 cut(s) 193, 518, 846, 945, 1152, 1182
Bst4CI ACNGT 9 cut(s) 323, 433, 467, 500, 607, 922, 1218, 1225, 1446
Bst6I CTCTTC 1 cut(s) 692
BstACI GRCGYC 1 cut(s) 132
BstAPI GCANNNNNTGC 1 cut(s) 47
BstBI TTCGAA 1 cut(s) 828
BstC8I GCNNGC 3 cut(s) 244, 662, 1205
BstDEI CTNAG 3 cut(s) 259, 655, 723
BstDSI CCRYGG 1 cut(s) 1113
BstENI CCTNNNNNAGG 1 cut(s) 515
BstF5I GGATG 3 cut(s) 81, 841, 1435
BstH2I RGCGCY 2 cut(s) 105, 135
BstHHI GCGC 2 cut(s) 104, 134
BstKTI GATC 5 cut(s) 292, 856, 901, 1251, 1435
BstMAI GTCTC 2 cut(s) 562, 761
BstMBI GATC 5 cut(s) 289, 853, 898, 1248, 1432
BstMWI GCNNNNNNNGC 6 cut(s) 47, 131, 560, 717, 1051, 1416
BstNI CCWGG 6 cut(s) 193, 518, 846, 945, 1152, 1182
BstNSI RCATGY 1 cut(s) 694
BstSCI CCNGG 6 cut(s) 191, 516, 844, 943, 1150, 1180
BstSFI CTRYAG 2 cut(s) 603, 921
BstV1I GCAGC 4 cut(s) 723, 1042, 1283, 1431
BstX2I RGATCY 1 cut(s) 1248
BstYI RGATCY 1 cut(s) 1248
BsuRI GGCC 4 cut(s) 660, 984, 1203, 1404
BtgI CCRYGG 1 cut(s) 1113
BtsCI GGATG 3 cut(s) 81, 841, 1435
BtsI GCAGTG 2 cut(s) 230, 1100
BtsIMutI CAGTG 5 cut(s) 230, 720, 918, 1100, 1395
Cac8I GCNNGC 3 cut(s) 244, 662, 1205
CfoI GCGC 2 cut(s) 104, 134
Cfr13I GGNCC 1 cut(s) 1201
Csp6I GTAC 5 cut(s) 335, 501, 800, 926, 1155
CviAII CATG 5 cut(s) 564, 691, 1114, 1424, 1436
CviQI GTAC 5 cut(s) 335, 501, 800, 926, 1155
DdeI CTNAG 3 cut(s) 259, 655, 723
DinI GGCGCC 1 cut(s) 133
DpnI GATC 5 cut(s) 291, 855, 900, 1250, 1434
DpnII GATC 5 cut(s) 289, 853, 898, 1248, 1432
EaeI YGGCCR 1 cut(s) 1402
Eam1104I CTCTTC 1 cut(s) 692
EarI CTCTTC 1 cut(s) 692
Ecl136II GAGCTC 1 cut(s) 1011
Eco130I CCWWGG 1 cut(s) 1113
Eco24I GRGCYC 1 cut(s) 1013
Eco53kI GAGCTC 1 cut(s) 1011
EcoICRI GAGCTC 1 cut(s) 1011
EcoNI CCTNNNNNAGG 1 cut(s) 515
EcoRII CCWGG 6 cut(s) 191, 516, 844, 943, 1150, 1180
EcoT14I CCWWGG 1 cut(s) 1113
EcoT38I GRGCYC 1 cut(s) 1013
EgeI GGCGCC 1 cut(s) 133
EheI GGCGCC 1 cut(s) 133
ErhI CCWWGG 1 cut(s) 1113
FaeI CATG 5 cut(s) 567, 694, 1117, 1427, 1439
FaqI GGGAC 1 cut(s) 102
FatI CATG 5 cut(s) 563, 690, 1113, 1423, 1435
FbaI TGATCA 3 cut(s) 853, 898, 1432
FblI GTMKAC 2 cut(s) 226, 1191
Fnu4HI GCNGC 4 cut(s) 712, 1031, 1272, 1420
FokI GGATG 3 cut(s) 88, 828, 1442
FriOI GRGCYC 1 cut(s) 1013
Fsp4HI GCNGC 4 cut(s) 712, 1031, 1272, 1420
FspBI CTAG 3 cut(s) 792, 891, 1074
GlaI GCGC 2 cut(s) 103, 133
GluI GCNGC 4 cut(s) 712, 1031, 1272, 1420
HaeII RGCGCY 2 cut(s) 105, 135
HaeIII GGCC 4 cut(s) 660, 984, 1203, 1404
HapII CCGG 2 cut(s) 34, 1334
HhaI GCGC 2 cut(s) 104, 134
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 5 cut(s) 567, 694, 1117, 1427, 1439
Hin6I GCGC 2 cut(s) 102, 132
HinP1I GCGC 2 cut(s) 102, 132
HincII GTYRAC 2 cut(s) 211, 1221
HindII GTYRAC 2 cut(s) 211, 1221
HindIII AAGCTT 1 cut(s) 527
HinfI GANTC 3 cut(s) 117, 453, 940
HpaII CCGG 2 cut(s) 34, 1334
HphI GGTGA 1 cut(s) 647
Hpy166II GTNNAC 6 cut(s) 211, 227, 337, 474, 1192, 1221
Hpy188I TCNGA 5 cut(s) 116, 583, 679, 784, 970
Hpy188III TCNNGA 3 cut(s) 293, 305, 595
Hpy8I GTNNAC 6 cut(s) 211, 227, 337, 474, 1192, 1221
Hpy99I CGWCG 1 cut(s) 130
HpyAV CCTTC 4 cut(s) 143, 199, 824, 1340
HpyCH4III ACNGT 9 cut(s) 323, 433, 467, 500, 607, 922, 1218, 1225, 1446
HpyF10VI GCNNNNNNNGC 6 cut(s) 47, 131, 560, 717, 1051, 1416
HpyF3I CTNAG 3 cut(s) 259, 655, 723
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 5 cut(s) 567, 694, 1117, 1427, 1439
HspAI GCGC 2 cut(s) 102, 132
KasI GGCGCC 1 cut(s) 131
Ksp22I TGATCA 3 cut(s) 853, 898, 1432
Kzo9I GATC 5 cut(s) 289, 853, 898, 1248, 1432
LmnI GCTCC 3 cut(s) 145, 583, 1016
Lsp1109I GCAGC 4 cut(s) 723, 1042, 1283, 1431
LweI GCATC 4 cut(s) 70, 127, 1042, 1323
MaeI CTAG 3 cut(s) 792, 891, 1074
MaeIII GTNAC 4 cut(s) 254, 323, 533, 1393
MalI GATC 5 cut(s) 291, 855, 900, 1250, 1434
MboI GATC 5 cut(s) 289, 853, 898, 1248, 1432
MboII GAAGA 4 cut(s) 681, 709, 923, 1028
MfeI CAATTG 2 cut(s) 616, 1143
MflI RGATCY 1 cut(s) 1248
MhlI GDGCHC 4 cut(s) 392, 588, 722, 1013
MlsI TGGCCA 1 cut(s) 1404
MluCI AATT 9 cut(s) 24, 94, 308, 316, 490, 616, 812, 870, 1143
MluNI TGGCCA 1 cut(s) 1404
Mly113I GGCGCC 1 cut(s) 132
MmeI TCCRAC 2 cut(s) 561, 657
Mox20I TGGCCA 1 cut(s) 1404
MscI TGGCCA 1 cut(s) 1404
MseI TTAA 4 cut(s) 27, 737, 1209, 1245
MslI CAYNNNNRTG 2 cut(s) 1286, 1428
Msp20I TGGCCA 1 cut(s) 1404
MspI CCGG 2 cut(s) 34, 1334
MspR9I CCNGG 6 cut(s) 193, 518, 846, 945, 1152, 1182
MunI CAATTG 2 cut(s) 616, 1143
MvaI CCWGG 6 cut(s) 193, 518, 846, 945, 1152, 1182
MwoI GCNNNNNNNGC 6 cut(s) 47, 131, 560, 717, 1051, 1416
NarI GGCGCC 1 cut(s) 132
NcoI CCATGG 1 cut(s) 1113
NdeII GATC 5 cut(s) 289, 853, 898, 1248, 1432
NlaIII CATG 5 cut(s) 567, 694, 1117, 1427, 1439
NlaIV GGNNCC 1 cut(s) 133
NmeAIII GCCGAG 1 cut(s) 320
NmuCI GTSAC 2 cut(s) 254, 1393
NspI RCATGY 1 cut(s) 694
NspV TTCGAA 1 cut(s) 828
PciI ACATGT 1 cut(s) 690
PfeI GAWTC 3 cut(s) 117, 453, 940
PfoI TCCNGGA 1 cut(s) 844
PkrI GCNGC 4 cut(s) 713, 1032, 1273, 1421
PluTI GGCGCC 1 cut(s) 135
PscI ACATGT 1 cut(s) 690
PsiI TTATAA 1 cut(s) 495
Psp124BI GAGCTC 1 cut(s) 1013
Psp6I CCWGG 6 cut(s) 191, 516, 844, 943, 1150, 1180
PspGI CCWGG 6 cut(s) 191, 516, 844, 943, 1150, 1180
PspN4I GGNNCC 1 cut(s) 133
PspPI GGNCC 1 cut(s) 1201
PsuI RGATCY 1 cut(s) 1248
RsaI GTAC 5 cut(s) 336, 502, 801, 927, 1156
RsaNI GTAC 5 cut(s) 335, 501, 800, 926, 1155
RseI CAYNNNNRTG 2 cut(s) 1286, 1428
SacI GAGCTC 1 cut(s) 1013
SaqAI TTAA 4 cut(s) 27, 737, 1209, 1245
SatI GCNGC 4 cut(s) 712, 1031, 1272, 1420
Sau3AI GATC 5 cut(s) 289, 853, 898, 1248, 1432
Sau96I GGNCC 1 cut(s) 1201
ScrFI CCNGG 6 cut(s) 193, 518, 846, 945, 1152, 1182
SduI GDGCHC 4 cut(s) 392, 588, 722, 1013
SfaNI GCATC 4 cut(s) 70, 127, 1042, 1323
SfcI CTRYAG 2 cut(s) 603, 921
SfoI GGCGCC 1 cut(s) 133
SfuI TTCGAA 1 cut(s) 828
SmiMI CAYNNNNRTG 2 cut(s) 1286, 1428
SmlI CTYRAG 2 cut(s) 371, 803
SmoI CTYRAG 2 cut(s) 371, 803
Sse9I AATT 9 cut(s) 24, 94, 308, 316, 490, 616, 812, 870, 1143
SsiI CCGC 1 cut(s) 1389
SspDI GGCGCC 1 cut(s) 131
SspMI CTAG 3 cut(s) 792, 891, 1074
SstI GAGCTC 1 cut(s) 1013
StyD4I CCNGG 6 cut(s) 191, 516, 844, 943, 1150, 1180
StyI CCWWGG 1 cut(s) 1113
TaaI ACNGT 9 cut(s) 323, 433, 467, 500, 607, 922, 1218, 1225, 1446
TaqI TCGA 2 cut(s) 828, 987
TasI AATT 9 cut(s) 24, 94, 308, 316, 490, 616, 812, 870, 1143
TatI WGTACW 3 cut(s) 334, 799, 925
TfiI GAWTC 3 cut(s) 117, 453, 940
Tru1I TTAA 4 cut(s) 27, 737, 1209, 1245
Tru9I TTAA 4 cut(s) 27, 737, 1209, 1245
TscAI CASTG 5 cut(s) 237, 727, 925, 1107, 1402
TseFI GTSAC 2 cut(s) 254, 1393
TseI GCWGC 4 cut(s) 711, 1030, 1271, 1419
Tsp45I GTSAC 2 cut(s) 254, 1393
TspDTI ATGAA 7 cut(s) 365, 428, 953, 977, 1270, 1310, 1365
TspRI CASTG 5 cut(s) 237, 727, 925, 1107, 1402
XagI CCTNNNNNAGG 1 cut(s) 515
XapI RAATTY 3 cut(s) 94, 308, 812
XceI RCATGY 1 cut(s) 694
XmiI GTMKAC 2 cut(s) 226, 1191
XspI CTAG 3 cut(s) 792, 891, 1074
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.