Rh2CG396700

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0001058
Physical Location & Seq
Forward (+)
53887247 .. 53890776
3530 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG396700.1

Sequence Viewer

Length: 1476 bp
ATGGACAAAATCGCAAAATACAAAATTAAACACCGGATTGCAAATAATGCAACACACGAGCATCAACTTGCAAGGATGTATAAATGGGGACAAAATTCCAGCGCCTCTGCTTTCGGATTCCCTGCGACGGGCGCCGATGCTCCACTGCATATGTTTGCTTCCAAGGTTGTCAGTAAAGGCTATGTTGATATTGTTAGGACTATTTTGAAAGGTTGCCCCAGGTTTGCTTGGAAGGTTGACAGCAATGGGTGTCTACCACTGCATATAGCTTGCGAAAAAGGTCACTTAGAGATAGCCAGAACACTATTGATGATTGATCCAGACCTTGCTCTTGAATTTGACAATTACCGTTACACACCAGTACACTCGGCTGTAATGAATGGTCAAATCAAAATCCTTGAGGAACTCCTTTTGTGCTCTCCCAAATGTTTAGCAAGGCTCACAGGAGATGGAGAAACTGTCTTTCATCTTTCAGTAAGATTCAATAGATACGGTGTGTTTACCTCTTTGGTAAAGAATTATAACAGTACCTATCTCCTCAACCAGGCTGACAAGCTTGGTAACACAATCTTACATCTTGCTGTCTCTGCATGGAACATTATATTGTCGGAGCACATCATCAGGAATACTACAGTTGATGTCAATTGTAGAAATCAAAGAGGTGAAACAGCAATGGACATCTTAGGCCAGCAAAGAAGAATGTCGGATATAAGAAACATGTTGAAGAGTTTTGGTGGCAGCAAGAGCACTGAGCTGTTGTCTTTAACACCAAAGATTGATGAGATAGATAATGAGACTACAACAACATCAGAAAGTCCTAGAGTTGTACTTGAGCAAGAATTTGAAATACTTTTCGAAGGTATCACACATCCAGGAAGTGATCAATATGATTGTGAAATTGGTATGGGGCGTATGGCTAGAAATGATCAACCAATGGAAGATTTCACTGTAGTACAAGAAAAGATGAATCCAGGTCATCAAGTTGTGATGAACTCTGACAATCTTTTTGGCCTCGATTGTTTTGAAACAAGAATGAGCTCCACAGCCTTGAATGAGCAGCATCTTCCAAAGCCAAGAGGGCAAAACAAAGTGGGAAAAACTAGGCAATATGAGATATATAGAGAGGCACTGCAAAATACCATGGACACAATAACATTAGTTGCCATTCCAATTGCCCCAGGTACTTTCACTACTGGTATATACCCTCCTGGTGGTGTCTACGAGGAGGGCCAGCTTAAAGGACAGTCAACGGTTGGCAGAACAACAGCTTTTAAGATCTTTGCAATATGCAACACAGCAGCACTTTTCATATCTATGTGCATTGTCATTGTCTTGGTCAGCATCATTCATCATTCCCTTCCGGCGAAAACCACTAATGAAACTACTGGTGGTTGCTCACAAAGTAATGTGGGTGGCGGTGTCACTGATGGCCACAGCTTTTATTGCAGCCATGTGGATGATCATGCCACACGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

491

Amino Acids

54.5

Weight (kDa)

6.65

Isoelectric Point (pI)

28.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 55 - 137 2.4e-12 Ankyrin repeats (3 copies)
Ank_2 PF12796 122 - 197 4.7e-06 Ankyrin repeats (3 copies)
PGG PF13962 375 - 448 1.9e-17 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 522
AccB1I GGYRCC 1 cut(s) 131
AccI GTMKAC 2 cut(s) 253, 1218
AciI CCGC 1 cut(s) 1416
AclWI GGATC 1 cut(s) 311
AcoI YGGCCR 1 cut(s) 1429
AcsI RAATTY 3 cut(s) 94, 335, 839
AcyI GRCGYC 1 cut(s) 132
AfaI GTAC 5 cut(s) 363, 529, 828, 954, 1183
AfiI CCNNNNNNNGG 4 cut(s) 127, 128, 544, 1211
AflIII ACRYGT 1 cut(s) 717
AgsI TTSAA 7 cut(s) 208, 335, 484, 724, 845, 1025, 1051
AjnI CCWGG 6 cut(s) 218, 543, 871, 970, 1177, 1207
AluBI AGCT 7 cut(s) 269, 556, 754, 1038, 1234, 1268, 1437
AluI AGCT 7 cut(s) 269, 556, 754, 1038, 1234, 1268, 1437
Alw21I GWGCWC 4 cut(s) 419, 615, 749, 1040
Alw26I GTCTC 2 cut(s) 589, 788
AlwI GGATC 1 cut(s) 311
AoxI GGCC 4 cut(s) 685, 1009, 1228, 1429
ApeKI GCWGC 4 cut(s) 738, 1057, 1298, 1446
ApoI RAATTY 3 cut(s) 94, 335, 839
ArsI GACNNNNNNTTYG 2 cut(s) 989, 1021
AspLEI GCGC 2 cut(s) 104, 134
AspS9I GGNCC 1 cut(s) 1228
AsuHPI GGTGA 1 cut(s) 674
AsuII TTCGAA 1 cut(s) 855
BalI TGGCCA 1 cut(s) 1431
BanI GGYRCC 1 cut(s) 131
BanII GRGCYC 1 cut(s) 1040
BauI CACGAG 1 cut(s) 56
Bbv12I GWGCWC 4 cut(s) 419, 615, 749, 1040
BbvI GCAGC 4 cut(s) 750, 1069, 1310, 1458
BccI CCATC 2 cut(s) 443, 1421
BciT130I CCWGG 6 cut(s) 220, 545, 873, 972, 1179, 1209
BclI TGATCA 3 cut(s) 880, 925, 1459
BcoDI GTCTC 2 cut(s) 589, 788
BfaI CTAG 3 cut(s) 819, 918, 1101
BfmI CTRYAG 2 cut(s) 630, 948
BfoI RGCGCY 2 cut(s) 105, 135
BglI GCCNNNNNGGC 1 cut(s) 1078
BglII AGATCT 1 cut(s) 1275
BisI GCNGC 4 cut(s) 739, 1058, 1299, 1447
BlsI GCNGC 4 cut(s) 740, 1059, 1300, 1448
Bme1390I CCNGG 6 cut(s) 220, 545, 873, 972, 1179, 1209
BmgT120I GGNCC 1 cut(s) 1228
BmiI GGNNCC 1 cut(s) 133
BmrFI CCNGG 6 cut(s) 220, 545, 873, 972, 1179, 1209
BmsI GCATC 4 cut(s) 70, 127, 1069, 1350
Bpu14I TTCGAA 1 cut(s) 855
BpuEI CTTGAG 2 cut(s) 419, 851
BsaHI GRCGYC 1 cut(s) 132
BsaJI CCNNGG 4 cut(s) 162, 218, 1140, 1177
BsaWI WCCGGW 1 cut(s) 33
BsaXI ACNNNNNCTCC 2 cut(s) 444, 474
Bsc4I CCNNNNNNNGG 4 cut(s) 127, 128, 544, 1211
Bse1I ACTGG 3 cut(s) 359, 1198, 1390
Bse3DI GCAATG 2 cut(s) 250, 678
BseBI CCWGG 6 cut(s) 220, 545, 873, 972, 1179, 1209
BseDI CCNNGG 4 cut(s) 162, 218, 1140, 1177
BseGI GGATG 3 cut(s) 81, 868, 1462
BseLI CCNNNNNNNGG 4 cut(s) 127, 128, 544, 1211
BseMI GCAATG 2 cut(s) 250, 678
BseMII CTCAG 1 cut(s) 741
BseNI ACTGG 3 cut(s) 359, 1198, 1390
BseRI GAGGAG 2 cut(s) 527, 1238
BseXI GCAGC 4 cut(s) 750, 1069, 1310, 1458
BshFI GGCC 4 cut(s) 687, 1011, 1230, 1431
BshNI GGYRCC 1 cut(s) 131
BsiHKAI GWGCWC 4 cut(s) 419, 615, 749, 1040
BsiSI CCGG 2 cut(s) 34, 1361
BslFI GGGAC 1 cut(s) 102
BslI CCNNNNNNNGG 4 cut(s) 127, 128, 544, 1211
BsmAI GTCTC 2 cut(s) 589, 788
BsmFI GGGAC 1 cut(s) 102
BsnI GGCC 4 cut(s) 687, 1011, 1230, 1431
Bsp119I TTCGAA 1 cut(s) 855
Bsp1286I GDGCHC 4 cut(s) 419, 615, 749, 1040
Bsp143I GATC 5 cut(s) 316, 880, 925, 1275, 1459
Bsp19I CCATGG 1 cut(s) 1140
BspACI CCGC 1 cut(s) 1416
BspANI GGCC 4 cut(s) 687, 1011, 1230, 1431
BspCNI CTCAG 1 cut(s) 742
BspLI GGNNCC 1 cut(s) 133
BspPI GGATC 1 cut(s) 311
BspT104I TTCGAA 1 cut(s) 855
BspT107I GGYRCC 1 cut(s) 131
BsrDI GCAATG 2 cut(s) 250, 678
BsrI ACTGG 3 cut(s) 359, 1198, 1390
BssECI CCNNGG 4 cut(s) 162, 218, 1140, 1177
BssMI GATC 5 cut(s) 316, 880, 925, 1275, 1459
BssNI GRCGYC 1 cut(s) 132
BssSI CACGAG 1 cut(s) 56
BssT1I CCWWGG 2 cut(s) 162, 1140
Bst2BI CACGAG 1 cut(s) 56
Bst2UI CCWGG 6 cut(s) 220, 545, 873, 972, 1179, 1209
Bst4CI ACNGT 9 cut(s) 350, 460, 494, 527, 634, 949, 1245, 1252, 1473
Bst6I CTCTTC 1 cut(s) 719
BstACI GRCGYC 1 cut(s) 132
BstAPI GCANNNNNTGC 1 cut(s) 47
BstBI TTCGAA 1 cut(s) 855
BstC8I GCNNGC 3 cut(s) 271, 689, 1232
BstDEI CTNAG 3 cut(s) 286, 682, 750
BstDSI CCRYGG 1 cut(s) 1140
BstENI CCTNNNNNAGG 1 cut(s) 542
BstF5I GGATG 3 cut(s) 81, 868, 1462
BstH2I RGCGCY 2 cut(s) 105, 135
BstHHI GCGC 2 cut(s) 104, 134
BstKTI GATC 5 cut(s) 319, 883, 928, 1278, 1462
BstMAI GTCTC 2 cut(s) 589, 788
BstMBI GATC 5 cut(s) 316, 880, 925, 1275, 1459
BstMWI GCNNNNNNNGC 6 cut(s) 47, 131, 587, 744, 1078, 1443
BstNI CCWGG 6 cut(s) 220, 545, 873, 972, 1179, 1209
BstNSI RCATGY 1 cut(s) 721
BstSCI CCNGG 6 cut(s) 218, 543, 871, 970, 1177, 1207
BstSFI CTRYAG 2 cut(s) 630, 948
BstV1I GCAGC 4 cut(s) 750, 1069, 1310, 1458
BstX2I RGATCY 1 cut(s) 1275
BstYI RGATCY 1 cut(s) 1275
BsuRI GGCC 4 cut(s) 687, 1011, 1230, 1431
BtgI CCRYGG 1 cut(s) 1140
BtsCI GGATG 3 cut(s) 81, 868, 1462
BtsI GCAGTG 3 cut(s) 143, 257, 1127
BtsIMutI CAGTG 6 cut(s) 143, 257, 747, 945, 1127, 1422
Cac8I GCNNGC 3 cut(s) 271, 689, 1232
CfoI GCGC 2 cut(s) 104, 134
Cfr13I GGNCC 1 cut(s) 1228
Csp6I GTAC 5 cut(s) 362, 528, 827, 953, 1182
CviAII CATG 5 cut(s) 591, 718, 1141, 1451, 1463
CviQI GTAC 5 cut(s) 362, 528, 827, 953, 1182
DdeI CTNAG 3 cut(s) 286, 682, 750
DinI GGCGCC 1 cut(s) 133
DpnI GATC 5 cut(s) 318, 882, 927, 1277, 1461
DpnII GATC 5 cut(s) 316, 880, 925, 1275, 1459
EaeI YGGCCR 1 cut(s) 1429
Eam1104I CTCTTC 1 cut(s) 719
EarI CTCTTC 1 cut(s) 719
Ecl136II GAGCTC 1 cut(s) 1038
Eco130I CCWWGG 2 cut(s) 162, 1140
Eco24I GRGCYC 1 cut(s) 1040
Eco53kI GAGCTC 1 cut(s) 1038
EcoICRI GAGCTC 1 cut(s) 1038
EcoNI CCTNNNNNAGG 1 cut(s) 542
EcoRII CCWGG 6 cut(s) 218, 543, 871, 970, 1177, 1207
EcoT14I CCWWGG 2 cut(s) 162, 1140
EcoT38I GRGCYC 1 cut(s) 1040
EgeI GGCGCC 1 cut(s) 133
EheI GGCGCC 1 cut(s) 133
ErhI CCWWGG 2 cut(s) 162, 1140
FaeI CATG 5 cut(s) 594, 721, 1144, 1454, 1466
FaqI GGGAC 1 cut(s) 102
FatI CATG 5 cut(s) 590, 717, 1140, 1450, 1462
FauNDI CATATG 1 cut(s) 150
FbaI TGATCA 3 cut(s) 880, 925, 1459
FblI GTMKAC 2 cut(s) 253, 1218
Fnu4HI GCNGC 4 cut(s) 739, 1058, 1299, 1447
FokI GGATG 3 cut(s) 88, 855, 1469
FriOI GRGCYC 1 cut(s) 1040
Fsp4HI GCNGC 4 cut(s) 739, 1058, 1299, 1447
FspBI CTAG 3 cut(s) 819, 918, 1101
GlaI GCGC 2 cut(s) 103, 133
GluI GCNGC 4 cut(s) 739, 1058, 1299, 1447
HaeII RGCGCY 2 cut(s) 105, 135
HaeIII GGCC 4 cut(s) 687, 1011, 1230, 1431
HapII CCGG 2 cut(s) 34, 1361
HhaI GCGC 2 cut(s) 104, 134
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 5 cut(s) 594, 721, 1144, 1454, 1466
Hin6I GCGC 2 cut(s) 102, 132
HinP1I GCGC 2 cut(s) 102, 132
HincII GTYRAC 2 cut(s) 238, 1248
HindII GTYRAC 2 cut(s) 238, 1248
HindIII AAGCTT 1 cut(s) 554
HinfI GANTC 3 cut(s) 117, 480, 967
HpaII CCGG 2 cut(s) 34, 1361
HphI GGTGA 1 cut(s) 674
Hpy166II GTNNAC 6 cut(s) 238, 254, 364, 501, 1219, 1248
Hpy188I TCNGA 5 cut(s) 116, 610, 706, 811, 997
Hpy188III TCNNGA 3 cut(s) 320, 332, 622
Hpy8I GTNNAC 6 cut(s) 238, 254, 364, 501, 1219, 1248
Hpy99I CGWCG 1 cut(s) 130
HpyAV CCTTC 3 cut(s) 226, 851, 1367
HpyCH4III ACNGT 9 cut(s) 350, 460, 494, 527, 634, 949, 1245, 1252, 1473
HpyF10VI GCNNNNNNNGC 6 cut(s) 47, 131, 587, 744, 1078, 1443
HpyF3I CTNAG 3 cut(s) 286, 682, 750
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 5 cut(s) 594, 721, 1144, 1454, 1466
HspAI GCGC 2 cut(s) 102, 132
KasI GGCGCC 1 cut(s) 131
Ksp22I TGATCA 3 cut(s) 880, 925, 1459
Kzo9I GATC 5 cut(s) 316, 880, 925, 1275, 1459
LmnI GCTCC 3 cut(s) 145, 610, 1043
Lsp1109I GCAGC 4 cut(s) 750, 1069, 1310, 1458
LweI GCATC 4 cut(s) 70, 127, 1069, 1350
MaeI CTAG 3 cut(s) 819, 918, 1101
MaeIII GTNAC 4 cut(s) 281, 350, 560, 1420
MalI GATC 5 cut(s) 318, 882, 927, 1277, 1461
MboI GATC 5 cut(s) 316, 880, 925, 1275, 1459
MboII GAAGA 4 cut(s) 708, 736, 950, 1055
MfeI CAATTG 2 cut(s) 643, 1170
MflI RGATCY 1 cut(s) 1275
MhlI GDGCHC 4 cut(s) 419, 615, 749, 1040
MlsI TGGCCA 1 cut(s) 1431
MluCI AATT 9 cut(s) 24, 94, 335, 343, 517, 643, 839, 897, 1170
MluNI TGGCCA 1 cut(s) 1431
Mly113I GGCGCC 1 cut(s) 132
MmeI TCCRAC 2 cut(s) 588, 684
Mox20I TGGCCA 1 cut(s) 1431
MscI TGGCCA 1 cut(s) 1431
MseI TTAA 4 cut(s) 27, 764, 1236, 1272
MslI CAYNNNNRTG 2 cut(s) 1313, 1455
Msp20I TGGCCA 1 cut(s) 1431
MspI CCGG 2 cut(s) 34, 1361
MspR9I CCNGG 6 cut(s) 220, 545, 873, 972, 1179, 1209
MunI CAATTG 2 cut(s) 643, 1170
MvaI CCWGG 6 cut(s) 220, 545, 873, 972, 1179, 1209
MwoI GCNNNNNNNGC 6 cut(s) 47, 131, 587, 744, 1078, 1443
NarI GGCGCC 1 cut(s) 132
NcoI CCATGG 1 cut(s) 1140
NdeI CATATG 1 cut(s) 150
NdeII GATC 5 cut(s) 316, 880, 925, 1275, 1459
NlaIII CATG 5 cut(s) 594, 721, 1144, 1454, 1466
NlaIV GGNNCC 1 cut(s) 133
NmeAIII GCCGAG 1 cut(s) 347
NmuCI GTSAC 2 cut(s) 281, 1420
NspI RCATGY 1 cut(s) 721
NspV TTCGAA 1 cut(s) 855
PciI ACATGT 1 cut(s) 717
PfeI GAWTC 3 cut(s) 117, 480, 967
PfoI TCCNGGA 1 cut(s) 871
PkrI GCNGC 4 cut(s) 740, 1059, 1300, 1448
PluTI GGCGCC 1 cut(s) 135
PscI ACATGT 1 cut(s) 717
PsiI TTATAA 1 cut(s) 522
Psp124BI GAGCTC 1 cut(s) 1040
Psp6I CCWGG 6 cut(s) 218, 543, 871, 970, 1177, 1207
PspGI CCWGG 6 cut(s) 218, 543, 871, 970, 1177, 1207
PspN4I GGNNCC 1 cut(s) 133
PspPI GGNCC 1 cut(s) 1228
PsuI RGATCY 1 cut(s) 1275
RsaI GTAC 5 cut(s) 363, 529, 828, 954, 1183
RsaNI GTAC 5 cut(s) 362, 528, 827, 953, 1182
RseI CAYNNNNRTG 2 cut(s) 1313, 1455
SacI GAGCTC 1 cut(s) 1040
SaqAI TTAA 4 cut(s) 27, 764, 1236, 1272
SatI GCNGC 4 cut(s) 739, 1058, 1299, 1447
Sau3AI GATC 5 cut(s) 316, 880, 925, 1275, 1459
Sau96I GGNCC 1 cut(s) 1228
ScrFI CCNGG 6 cut(s) 220, 545, 873, 972, 1179, 1209
SduI GDGCHC 4 cut(s) 419, 615, 749, 1040
SfaNI GCATC 4 cut(s) 70, 127, 1069, 1350
SfcI CTRYAG 2 cut(s) 630, 948
SfoI GGCGCC 1 cut(s) 133
SfuI TTCGAA 1 cut(s) 855
SmiMI CAYNNNNRTG 2 cut(s) 1313, 1455
SmlI CTYRAG 2 cut(s) 398, 830
SmoI CTYRAG 2 cut(s) 398, 830
Sse9I AATT 9 cut(s) 24, 94, 335, 343, 517, 643, 839, 897, 1170
SsiI CCGC 1 cut(s) 1416
SspDI GGCGCC 1 cut(s) 131
SspMI CTAG 3 cut(s) 819, 918, 1101
SstI GAGCTC 1 cut(s) 1040
StyD4I CCNGG 6 cut(s) 218, 543, 871, 970, 1177, 1207
StyI CCWWGG 2 cut(s) 162, 1140
TaaI ACNGT 9 cut(s) 350, 460, 494, 527, 634, 949, 1245, 1252, 1473
TaqI TCGA 2 cut(s) 855, 1014
TasI AATT 9 cut(s) 24, 94, 335, 343, 517, 643, 839, 897, 1170
TatI WGTACW 3 cut(s) 361, 826, 952
TfiI GAWTC 3 cut(s) 117, 480, 967
Tru1I TTAA 4 cut(s) 27, 764, 1236, 1272
Tru9I TTAA 4 cut(s) 27, 764, 1236, 1272
TscAI CASTG 6 cut(s) 150, 264, 754, 952, 1134, 1429
TseFI GTSAC 2 cut(s) 281, 1420
TseI GCWGC 4 cut(s) 738, 1057, 1298, 1446
Tsp45I GTSAC 2 cut(s) 281, 1420
TspDTI ATGAA 7 cut(s) 392, 455, 980, 1004, 1297, 1337, 1392
TspRI CASTG 6 cut(s) 150, 264, 754, 952, 1134, 1429
XagI CCTNNNNNAGG 1 cut(s) 542
XapI RAATTY 3 cut(s) 94, 335, 839
XceI RCATGY 1 cut(s) 721
XmiI GTMKAC 2 cut(s) 253, 1218
XspI CTAG 3 cut(s) 819, 918, 1101
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.