Rh2BG421100

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0001058
Physical Location & Seq
Forward (+)
59218325 .. 59219937
1613 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG421100.1

Sequence Viewer

Length: 1434 bp
ATGAAAGGACTGTACTCCCAACTTCATAACATATTACGGTGCATTGCTCAACATCCTAATATTCTCTTTGGAAATCTGGAAATCTGGACTTTCATCGACTTGGTTGAAAACAATGGAGGTTCTCTCATTCAGAAAACTGAGGAAACAAAAAACTCGGTGTTACACCTTGCTGCAAGGTTCGGCCACGTTGAGCTGGTTGCCTATATCACTAAGCGGCTTCCTGACTTGGTTTCCGTTGGAAACATTAAGGGAGAAACTCCATTACATGAAGCTTGTCGCCAAGGGAATACCAGTGTTGTAAAGCTGCTGTTAGAGGTCAATCCATATGTGGCTTGTAGTCTGAACCACGAAATGCAAACTGCTTTCTACATGGCTTGTAGTCATGGAAATGTTGATGTTGTGAAGCACATGACAAACCAACCAGGGGTGTTAGACGCTGAAGAACATGAAGTCTGCAGCCCTCTACATCTAGCTATAGCAGAAGGCTTTATCGATATTGTTAAGAGTATTTTAGAAGCTTGCCCCAAGTTTGCTTGGAAGGTTGACAGCAAAGGACTTTTGCCGCTCCATTTGGCTTGCGAAACAGGTCACGTAGAGATAACAAGAACACTCTTGACGATTGATCCAAACCTTGCTCTTGAGTTTAACCCTTACGGTTACACACCACTACACTTGGCTGCGATGAATGGTCAAATCCAAATCCTTGAGGAACTTTTACTTTGCTCTCCCAAATCTTTAGAACTTCTCACACGAGACAGGGAAACTGTCTTTCATCTTTCAGTAAGATTCAACAGATACAGTATGTTTACCTCCTTGGCAAAGAATTATAATGACACTAACCTCCTCAACCAGGCTGACAAGCATGGTAACACAATCTTACATCTCACCGTCGCTGCAGGGAACACTAGATTATCGGAGTACATCATCAGAAATACTACGGTAGATGTCAATTTTAAGAACCAAAGAGGTGAAACAGCGTTGGACATCCTAAATCAGCAAAGAACATTATCTGATCTTAGAAACATGTTGAAGAGTTTTGGTGGCAGGACAAGTACTGAGCTGTTCCCTTTAACCCCAAAGGTTGATGAGATAGATTCTGAGACTACAACATTAGAAAGTCCTAGAGCTGCACTTGAGCAAGAATTTGAAATGCTTTTTGAAGGTAATATGCAACCAGGAAGTGATCAATATGATTGTGAAATTGGCAGGGGGCGCATGGCTAGAAACTGTACTGGTGTTATCCCATTCATGATGGATTCAATCCTCAGAAATAATCAACCACTGGAAGAATTCACTACAGTACAAGCGAAGATGAATCCATGTCATCAAGTTGTGATGAACTCTGATAATGATTTTGGCTCAAATTCTTCTAGAACAAGAATGAGCTCCCAAGTCTCGAATCCAAAGCAGGTTCCGAAGACGAGGAAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

477

Amino Acids

53.54

Weight (kDa)

6.03

Isoelectric Point (pI)

34.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 45 - 105 6.9e-10 Ankyrin repeats (3 copies)
Ank_4 PF13637 54 - 104 1.4e-08 Ankyrin repeats (many copies)
Ank PF00023 84 - 107 5.4e-06 Ankyrin repeat
Ank_2 PF12796 116 - 172 6.2e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 155 - 239 3.2e-15 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 828
Acc36I ACCTGC 1 cut(s) 1399
AccBSI CCGCTC 1 cut(s) 565
AciI CCGC 2 cut(s) 214, 563
AclWI GGATC 1 cut(s) 617
AcoI YGGCCR 1 cut(s) 181
AcsI RAATTY 3 cut(s) 1142, 1289, 1363
AcuI CTGAAG 1 cut(s) 459
AfaI GTAC 5 cut(s) 14, 920, 1054, 1231, 1302
AfiI CCNNNNNNNGG 2 cut(s) 424, 850
AflIII ACRYGT 1 cut(s) 1023
AgsI TTSAA 6 cut(s) 107, 790, 1030, 1148, 1160, 1260
AjnI CCWGG 3 cut(s) 421, 849, 1174
AluBI AGCT 8 cut(s) 193, 272, 304, 473, 518, 1060, 1127, 1386
AluI AGCT 8 cut(s) 193, 272, 304, 473, 518, 1060, 1127, 1386
Alw21I GWGCWC 1 cut(s) 1388
Alw26I GTCTC 3 cut(s) 747, 1094, 1399
AlwI GGATC 1 cut(s) 617
AoxI GGCC 1 cut(s) 181
ApeKI GCWGC 6 cut(s) 170, 304, 456, 677, 893, 1127
ApoI RAATTY 3 cut(s) 1142, 1289, 1363
AspLEI GCGC 1 cut(s) 1215
AsuHPI GGTGA 2 cut(s) 877, 980
BanII GRGCYC 1 cut(s) 1388
BauI CACGAG 1 cut(s) 750
BbsI GAAGAC 1 cut(s) 1424
Bbv12I GWGCWC 1 cut(s) 1388
BbvI GCAGC 6 cut(s) 157, 291, 468, 664, 880, 1114
BccI CCATC 1 cut(s) 1246
BciT130I CCWGG 3 cut(s) 423, 851, 1176
BclI TGATCA 1 cut(s) 1183
BcoDI GTCTC 3 cut(s) 747, 1094, 1399
BfaI CTAG 5 cut(s) 470, 906, 1122, 1221, 1371
BfmI CTRYAG 4 cut(s) 454, 474, 894, 1296
BfuAI ACCTGC 1 cut(s) 1399
BisI GCNGC 8 cut(s) 171, 215, 305, 457, 563, 678, 894, 1128
BlsI GCNGC 8 cut(s) 172, 216, 306, 458, 564, 679, 895, 1129
BmcAI AGTACT 1 cut(s) 1054
Bme1390I CCNGG 3 cut(s) 423, 851, 1176
BmiI GGNNCC 1 cut(s) 1413
BmrFI CCNGG 3 cut(s) 423, 851, 1176
BpiI GAAGAC 1 cut(s) 1424
BplI GAGNNNNNCTC 2 cut(s) 108, 140
BpuEI CTTGAG 3 cut(s) 659, 725, 1154
Bsa29I ATCGAT 1 cut(s) 492
BsaAI YACGTR 1 cut(s) 592
BsaJI CCNNGG 3 cut(s) 280, 422, 813
Bsc4I CCNNNNNNNGG 2 cut(s) 424, 850
Bse1I ACTGG 3 cut(s) 291, 1237, 1287
Bse3DI GCAATG 1 cut(s) 42
BseBI CCWGG 3 cut(s) 423, 851, 1176
BseCI ATCGAT 1 cut(s) 492
BseDI CCNNGG 3 cut(s) 280, 422, 813
BseGI GGATG 2 cut(s) 52, 984
BseLI CCNNNNNNNGG 2 cut(s) 424, 850
BseMI GCAATG 1 cut(s) 42
BseMII CTCAG 4 cut(s) 129, 1047, 1089, 1279
BseNI ACTGG 3 cut(s) 291, 1237, 1287
BseRI GAGGAG 1 cut(s) 833
BseXI GCAGC 6 cut(s) 157, 291, 468, 664, 880, 1114
BsgI GTGCAG 1 cut(s) 1113
BshFI GGCC 1 cut(s) 183
BshVI ATCGAT 1 cut(s) 492
BsiHKAI GWGCWC 1 cut(s) 1388
BslI CCNNNNNNNGG 2 cut(s) 424, 850
BsmAI GTCTC 3 cut(s) 747, 1094, 1399
BsnI GGCC 1 cut(s) 183
Bsp1286I GDGCHC 1 cut(s) 1388
Bsp143I GATC 3 cut(s) 622, 1012, 1183
BspACI CCGC 2 cut(s) 214, 563
BspANI GGCC 1 cut(s) 183
BspCNI CTCAG 4 cut(s) 130, 1048, 1090, 1278
BspDI ATCGAT 1 cut(s) 492
BspHI TCATGA 1 cut(s) 1248
BspLI GGNNCC 1 cut(s) 1413
BspMAI CTGCAG 2 cut(s) 458, 898
BspMI ACCTGC 1 cut(s) 1399
BspPI GGATC 1 cut(s) 617
BsrBI CCGCTC 1 cut(s) 565
BsrDI GCAATG 1 cut(s) 42
BsrI ACTGG 3 cut(s) 291, 1237, 1287
BssECI CCNNGG 3 cut(s) 280, 422, 813
BssMI GATC 3 cut(s) 622, 1012, 1183
BssSI CACGAG 1 cut(s) 750
BssT1I CCWWGG 2 cut(s) 280, 813
Bst2BI CACGAG 1 cut(s) 750
Bst2UI CCWGG 3 cut(s) 423, 851, 1176
Bst4CI ACNGT 9 cut(s) 12, 39, 656, 766, 800, 889, 940, 1229, 1300
Bst6I CTCTTC 1 cut(s) 1025
BstBAI YACGTR 1 cut(s) 592
BstC8I GCNNGC 2 cut(s) 520, 577
BstDEI CTNAG 6 cut(s) 138, 210, 1016, 1056, 1098, 1265
BstENI CCTNNNNNAGG 1 cut(s) 848
BstF5I GGATG 2 cut(s) 52, 984
BstHHI GCGC 1 cut(s) 1215
BstKTI GATC 3 cut(s) 625, 1015, 1186
BstMAI GTCTC 3 cut(s) 747, 1094, 1399
BstMBI GATC 3 cut(s) 622, 1012, 1183
BstMWI GCNNNNNNNGC 1 cut(s) 1212
BstNI CCWGG 3 cut(s) 423, 851, 1176
BstNSI RCATGY 1 cut(s) 1027
BstSCI CCNGG 3 cut(s) 421, 849, 1174
BstSFI CTRYAG 4 cut(s) 454, 474, 894, 1296
BstV1I GCAGC 6 cut(s) 157, 291, 468, 664, 880, 1114
BstV2I GAAGAC 1 cut(s) 1424
Bsu15I ATCGAT 1 cut(s) 492
BsuRI GGCC 1 cut(s) 183
BsuTUI ATCGAT 1 cut(s) 492
BtgZI GCGATG 1 cut(s) 695
BtsCI GGATG 2 cut(s) 52, 984
BtsIMutI CAGTG 2 cut(s) 298, 1280
BveI ACCTGC 1 cut(s) 1399
Cac8I GCNNGC 2 cut(s) 520, 577
CciI TCATGA 1 cut(s) 1248
CfoI GCGC 1 cut(s) 1215
ClaI ATCGAT 1 cut(s) 492
CseI GACGC 1 cut(s) 443
Csp6I GTAC 5 cut(s) 13, 919, 1053, 1230, 1301
CspCI CAANNNNNGTGG 2 cut(s) 654, 689
CviQI GTAC 5 cut(s) 13, 919, 1053, 1230, 1301
DdeI CTNAG 6 cut(s) 138, 210, 1016, 1056, 1098, 1265
DpnI GATC 3 cut(s) 624, 1014, 1185
DpnII GATC 3 cut(s) 622, 1012, 1183
EaeI YGGCCR 1 cut(s) 181
Eam1104I CTCTTC 1 cut(s) 1025
EarI CTCTTC 1 cut(s) 1025
Ecl136II GAGCTC 1 cut(s) 1386
Eco130I CCWWGG 2 cut(s) 280, 813
Eco24I GRGCYC 1 cut(s) 1388
Eco53kI GAGCTC 1 cut(s) 1386
Eco57I CTGAAG 1 cut(s) 459
EcoICRI GAGCTC 1 cut(s) 1386
EcoNI CCTNNNNNAGG 1 cut(s) 848
EcoRI GAATTC 1 cut(s) 1289
EcoRII CCWGG 3 cut(s) 421, 849, 1174
EcoT14I CCWWGG 2 cut(s) 280, 813
EcoT38I GRGCYC 1 cut(s) 1388
ErhI CCWWGG 2 cut(s) 280, 813
FauNDI CATATG 1 cut(s) 325
FbaI TGATCA 1 cut(s) 1183
Fnu4HI GCNGC 8 cut(s) 171, 215, 305, 457, 563, 678, 894, 1128
FokI GGATG 2 cut(s) 39, 971
FriOI GRGCYC 1 cut(s) 1388
Fsp4HI GCNGC 8 cut(s) 171, 215, 305, 457, 563, 678, 894, 1128
FspBI CTAG 5 cut(s) 470, 906, 1122, 1221, 1371
GlaI GCGC 1 cut(s) 1214
GluI GCNGC 8 cut(s) 171, 215, 305, 457, 563, 678, 894, 1128
HaeIII GGCC 1 cut(s) 183
HgaI GACGC 1 cut(s) 443
HhaI GCGC 1 cut(s) 1215
Hin6I GCGC 1 cut(s) 1213
HinP1I GCGC 1 cut(s) 1213
HincII GTYRAC 1 cut(s) 544
HindII GTYRAC 1 cut(s) 544
HindIII AAGCTT 2 cut(s) 270, 516
HinfI GANTC 5 cut(s) 786, 1094, 1256, 1315, 1399
HphI GGTGA 2 cut(s) 877, 980
Hpy166II GTNNAC 2 cut(s) 544, 807
Hpy188I TCNGA 9 cut(s) 132, 342, 916, 929, 1012, 1099, 1268, 1345, 1416
Hpy188III TCNNGA 8 cut(s) 77, 85, 221, 613, 638, 1249, 1371, 1396
Hpy8I GTNNAC 2 cut(s) 544, 807
Hpy99I CGWCG 1 cut(s) 893
HpyAV CCTTC 3 cut(s) 476, 532, 1154
HpyCH4III ACNGT 9 cut(s) 12, 39, 656, 766, 800, 889, 940, 1229, 1300
HpyCH4IV ACGT 2 cut(s) 186, 591
HpyCH4V TGCA 7 cut(s) 42, 173, 355, 456, 896, 1130, 1171
HpyF10VI GCNNNNNNNGC 1 cut(s) 1212
HpyF3I CTNAG 6 cut(s) 138, 210, 1016, 1056, 1098, 1265
HpySE526I ACGT 2 cut(s) 186, 591
HspAI GCGC 1 cut(s) 1213
Ksp22I TGATCA 1 cut(s) 1183
Kzo9I GATC 3 cut(s) 622, 1012, 1183
LmnI GCTCC 2 cut(s) 570, 1391
Lsp1109I GCAGC 6 cut(s) 157, 291, 468, 664, 880, 1114
MaeI CTAG 5 cut(s) 470, 906, 1122, 1221, 1371
MaeII ACGT 2 cut(s) 186, 591
MaeIII GTNAC 4 cut(s) 159, 587, 656, 866
MalI GATC 3 cut(s) 624, 1014, 1185
MbiI CCGCTC 1 cut(s) 565
MboI GATC 3 cut(s) 622, 1012, 1183
MboII GAAGA 6 cut(s) 452, 1042, 1298, 1321, 1359, 1429
MhlI GDGCHC 1 cut(s) 1388
MluCI AATT 6 cut(s) 823, 949, 1142, 1200, 1289, 1363
MmeI TCCRAC 2 cut(s) 217, 960
MseI TTAA 5 cut(s) 246, 501, 645, 954, 1070
MslI CAYNNNNRTG 1 cut(s) 387
MspR9I CCNGG 3 cut(s) 423, 851, 1176
MvaI CCWGG 3 cut(s) 423, 851, 1176
MwoI GCNNNNNNNGC 1 cut(s) 1212
NdeI CATATG 1 cut(s) 325
NdeII GATC 3 cut(s) 622, 1012, 1183
NlaIV GGNNCC 1 cut(s) 1413
NmuCI GTSAC 1 cut(s) 587
NspI RCATGY 1 cut(s) 1027
PagI TCATGA 1 cut(s) 1248
PciI ACATGT 1 cut(s) 1023
PfeI GAWTC 5 cut(s) 786, 1094, 1256, 1315, 1399
PkrI GCNGC 8 cut(s) 172, 216, 306, 458, 564, 679, 895, 1129
Ppu21I YACGTR 1 cut(s) 592
PscI ACATGT 1 cut(s) 1023
PsiI TTATAA 1 cut(s) 828
Psp124BI GAGCTC 1 cut(s) 1388
Psp6I CCWGG 3 cut(s) 421, 849, 1174
PspGI CCWGG 3 cut(s) 421, 849, 1174
PspN4I GGNNCC 1 cut(s) 1413
PstI CTGCAG 2 cut(s) 458, 898
RsaI GTAC 5 cut(s) 14, 920, 1054, 1231, 1302
RsaNI GTAC 5 cut(s) 13, 919, 1053, 1230, 1301
RseI CAYNNNNRTG 1 cut(s) 387
SacI GAGCTC 1 cut(s) 1388
SaqAI TTAA 5 cut(s) 246, 501, 645, 954, 1070
SatI GCNGC 8 cut(s) 171, 215, 305, 457, 563, 678, 894, 1128
Sau3AI GATC 3 cut(s) 622, 1012, 1183
ScaI AGTACT 1 cut(s) 1054
ScrFI CCNGG 3 cut(s) 423, 851, 1176
SduI GDGCHC 1 cut(s) 1388
SfcI CTRYAG 4 cut(s) 454, 474, 894, 1296
SmiMI CAYNNNNRTG 1 cut(s) 387
SmlI CTYRAG 3 cut(s) 638, 704, 1133
SmoI CTYRAG 3 cut(s) 638, 704, 1133
Sse9I AATT 6 cut(s) 823, 949, 1142, 1200, 1289, 1363
SsiI CCGC 2 cut(s) 214, 563
SspI AATATT 1 cut(s) 61
SspMI CTAG 5 cut(s) 470, 906, 1122, 1221, 1371
SstI GAGCTC 1 cut(s) 1388
StyD4I CCNGG 3 cut(s) 421, 849, 1174
StyI CCWWGG 2 cut(s) 280, 813
TaaI ACNGT 9 cut(s) 12, 39, 656, 766, 800, 889, 940, 1229, 1300
TaiI ACGT 2 cut(s) 189, 594
TaqI TCGA 3 cut(s) 96, 492, 1397
TasI AATT 6 cut(s) 823, 949, 1142, 1200, 1289, 1363
TatI WGTACW 5 cut(s) 12, 918, 1052, 1229, 1300
TauI GCSGC 2 cut(s) 217, 565
TfiI GAWTC 5 cut(s) 786, 1094, 1256, 1315, 1399
Tru1I TTAA 5 cut(s) 246, 501, 645, 954, 1070
Tru9I TTAA 5 cut(s) 246, 501, 645, 954, 1070
TscAI CASTG 2 cut(s) 298, 1287
TseFI GTSAC 1 cut(s) 587
TseI GCWGC 6 cut(s) 170, 304, 456, 677, 893, 1127
Tsp45I GTSAC 1 cut(s) 587
TspGWI ACGGA 1 cut(s) 223
TspRI CASTG 2 cut(s) 298, 1287
XagI CCTNNNNNAGG 1 cut(s) 848
XapI RAATTY 3 cut(s) 1142, 1289, 1363
XbaI TCTAGA 1 cut(s) 1370
XceI RCATGY 1 cut(s) 1027
XspI CTAG 5 cut(s) 470, 906, 1122, 1221, 1371
ZrmI AGTACT 1 cut(s) 1054
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.