Rh2DG430300

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0001058
Physical Location & Seq
Forward (+)
62116802 .. 62119706
2905 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG430300.1

Sequence Viewer

Length: 1245 bp
ATGGGGACAAAATTCCAGCGCCTCTGCTTTCGGATTCCCTGCGACGGGCGCCGATGCTCCACGACTATTTTGAAAGGTTGCCCCAGGTTTGCTTGGAAGGTTGACAGCAATGGGTGTCTACCACTGCATATAGCTTGCGAAAAAGGTCACTTAGAGATAGCAAGAACACTGTTGATGATTGATCCAGACCTTGCTCTTGAATTTGACAATTACCGTTACACACCAGTACACTCGGCTGTAATGAACGGTCAAATCAAAATCCTTGAGGAACTCCTTTTGTGCTCTCCCAAATGTTTAGCAAGGCTCACAGGAGATGGAGAAACTGTCTTTCATCTTTCAGTAAGATTCAATAGGTACGGTGTGTTTACCTCTTTGGTAAAGAATTATAACAGTACCTATCTCCTCAACCAGGCTGACAAGCTTGGTAACACAATCTTACGTCTTGCTGTCTCTGCATGGAACATTACATTGTCGGAGCACATCATCAGGAATACTACAGTTGATGTCAATTGTGGAAATCAAAGAGGTGAAACAGCAATGGACATCTTAGGCCAGCAAAGAAGAATGTCGGATATAAGAAACATGTTGAAGAGTTTTGGTGGCAGCAAGAGCACTGAGCTGTTGTCTTTAACACCAAAGATTGATGAGATAGATAATGAGACTACAACAACATCAGAAAGTCCTAGAGTTGTACTTGAGCAAGAATTTGAAATGCTTTTCGAAGGTATCACACATCCAGGAAGTGATCAATATGATTGTGAAATTGGTATGGGGCGTATGGCTAGAAATGATCAACCAATGGAAGATTTCACTGTAGTACAAGAAAAGATGAATCCAGGTCATCAAGTTGTGATGAACTCTGACAATCTTTTTGGCCTCGATTGTTTTGAAACAAGAATGAGCTCCCCAGCCTTGAATGAGCAGCATCTTCCAAAGCCAAGAGGGCAAAACAAAGTGGGAAAAACTAGGCAATATGAGATATATAGAGATCAGGCACTGCAAAATACCATGGACACAATAACATTAGTTGCCATTCTAATTGCCCCAGGTACTTTCACTACTGGTATATACCCTCCTGGTGGTGTCTACGAGGAGGGCCAGCTTAAAGGACAGTCAACAGTTGGCAGAACAACAGCTTTTAAGATCTTTGCAATATGCAACACAGCAGCAATTTTTCATATCTATGTGCATTGTCATTGTCTTGGTCAGCATCATTCATCATTCCCTTCCGGCGAAAACCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

414

Amino Acids

46.44

Weight (kDa)

6.4

Isoelectric Point (pI)

35.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 33 - 92 2.7e-10 Ankyrin repeats (3 copies)
PGG PF13962 331 - 392 3.9e-13 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 387
AccB1I GGYRCC 1 cut(s) 48
AccI GTMKAC 2 cut(s) 118, 1086
AclWI GGATC 1 cut(s) 176
AcsI RAATTY 3 cut(s) 11, 200, 704
AcyI GRCGYC 1 cut(s) 49
AfaI GTAC 6 cut(s) 228, 356, 394, 693, 819, 1051
AfiI CCNNNNNNNGG 4 cut(s) 44, 45, 409, 1079
AflIII ACRYGT 1 cut(s) 582
AgsI TTSAA 7 cut(s) 73, 200, 349, 589, 710, 890, 916
AjnI CCWGG 6 cut(s) 83, 408, 736, 835, 1045, 1075
AluBI AGCT 6 cut(s) 134, 421, 619, 903, 1102, 1136
AluI AGCT 6 cut(s) 134, 421, 619, 903, 1102, 1136
Alw21I GWGCWC 4 cut(s) 284, 480, 614, 905
Alw26I GTCTC 2 cut(s) 454, 653
AlwI GGATC 1 cut(s) 176
AlwNI CAGNNNCTG 1 cut(s) 997
AoxI GGCC 3 cut(s) 550, 874, 1096
ApeKI GCWGC 3 cut(s) 603, 922, 1166
ApoI RAATTY 3 cut(s) 11, 200, 704
ArsI GACNNNNNNTTYG 2 cut(s) 854, 886
AspLEI GCGC 2 cut(s) 21, 51
AspS9I GGNCC 1 cut(s) 1096
AsuHPI GGTGA 1 cut(s) 539
AsuII TTCGAA 1 cut(s) 720
BanI GGYRCC 1 cut(s) 48
BanII GRGCYC 1 cut(s) 905
Bbv12I GWGCWC 4 cut(s) 284, 480, 614, 905
BbvI GCAGC 3 cut(s) 615, 934, 1178
BccI CCATC 1 cut(s) 308
BciT130I CCWGG 6 cut(s) 85, 410, 738, 837, 1047, 1077
BclI TGATCA 2 cut(s) 745, 790
BcoDI GTCTC 2 cut(s) 454, 653
BfaI CTAG 3 cut(s) 684, 783, 966
BfmI CTRYAG 2 cut(s) 495, 813
BfoI RGCGCY 2 cut(s) 22, 52
BglI GCCNNNNNGGC 1 cut(s) 943
BglII AGATCT 1 cut(s) 1143
BisI GCNGC 3 cut(s) 604, 923, 1167
BlsI GCNGC 3 cut(s) 605, 924, 1168
Bme1390I CCNGG 6 cut(s) 85, 410, 738, 837, 1047, 1077
BmgT120I GGNCC 1 cut(s) 1096
BmiI GGNNCC 1 cut(s) 50
BmrFI CCNGG 6 cut(s) 85, 410, 738, 837, 1047, 1077
BmsI GCATC 3 cut(s) 44, 934, 1219
Bpu14I TTCGAA 1 cut(s) 720
BpuEI CTTGAG 2 cut(s) 284, 716
BsaHI GRCGYC 1 cut(s) 49
BsaJI CCNNGG 3 cut(s) 83, 1008, 1045
BsaXI ACNNNNNCTCC 2 cut(s) 309, 339
Bsc4I CCNNNNNNNGG 4 cut(s) 44, 45, 409, 1079
Bse1I ACTGG 2 cut(s) 224, 1066
Bse3DI GCAATG 2 cut(s) 115, 543
BseBI CCWGG 6 cut(s) 85, 410, 738, 837, 1047, 1077
BseDI CCNNGG 3 cut(s) 83, 1008, 1045
BseGI GGATG 1 cut(s) 733
BseLI CCNNNNNNNGG 4 cut(s) 44, 45, 409, 1079
BseMI GCAATG 2 cut(s) 115, 543
BseMII CTCAG 1 cut(s) 606
BseNI ACTGG 2 cut(s) 224, 1066
BseRI GAGGAG 2 cut(s) 392, 1106
BseXI GCAGC 3 cut(s) 615, 934, 1178
BseYI CCCAGC 1 cut(s) 907
BshFI GGCC 3 cut(s) 552, 876, 1098
BshNI GGYRCC 1 cut(s) 48
BsiHKAI GWGCWC 4 cut(s) 284, 480, 614, 905
BsiSI CCGG 1 cut(s) 1230
BslFI GGGAC 1 cut(s) 19
BslI CCNNNNNNNGG 4 cut(s) 44, 45, 409, 1079
BsmAI GTCTC 2 cut(s) 454, 653
BsmFI GGGAC 1 cut(s) 19
BsnI GGCC 3 cut(s) 552, 876, 1098
Bsp119I TTCGAA 1 cut(s) 720
Bsp1286I GDGCHC 4 cut(s) 284, 480, 614, 905
Bsp143I GATC 5 cut(s) 181, 745, 790, 988, 1143
Bsp19I CCATGG 1 cut(s) 1008
BspANI GGCC 3 cut(s) 552, 876, 1098
BspCNI CTCAG 1 cut(s) 607
BspLI GGNNCC 1 cut(s) 50
BspPI GGATC 1 cut(s) 176
BspT104I TTCGAA 1 cut(s) 720
BspT107I GGYRCC 1 cut(s) 48
BsrDI GCAATG 2 cut(s) 115, 543
BsrI ACTGG 2 cut(s) 224, 1066
BssECI CCNNGG 3 cut(s) 83, 1008, 1045
BssMI GATC 5 cut(s) 181, 745, 790, 988, 1143
BssNI GRCGYC 1 cut(s) 49
BssT1I CCWWGG 1 cut(s) 1008
Bst2UI CCWGG 6 cut(s) 85, 410, 738, 837, 1047, 1077
Bst6I CTCTTC 1 cut(s) 584
BstACI GRCGYC 1 cut(s) 49
BstBI TTCGAA 1 cut(s) 720
BstC8I GCNNGC 3 cut(s) 136, 554, 1100
BstDEI CTNAG 3 cut(s) 151, 547, 615
BstDSI CCRYGG 1 cut(s) 1008
BstENI CCTNNNNNAGG 1 cut(s) 407
BstF5I GGATG 1 cut(s) 733
BstH2I RGCGCY 2 cut(s) 22, 52
BstHHI GCGC 2 cut(s) 21, 51
BstKTI GATC 5 cut(s) 184, 748, 793, 991, 1146
BstMAI GTCTC 2 cut(s) 454, 653
BstMBI GATC 5 cut(s) 181, 745, 790, 988, 1143
BstMWI GCNNNNNNNGC 4 cut(s) 48, 452, 609, 943
BstNI CCWGG 6 cut(s) 85, 410, 738, 837, 1047, 1077
BstNSI RCATGY 1 cut(s) 586
BstSCI CCNGG 6 cut(s) 83, 408, 736, 835, 1045, 1075
BstSFI CTRYAG 2 cut(s) 495, 813
BstV1I GCAGC 3 cut(s) 615, 934, 1178
BstX2I RGATCY 1 cut(s) 1143
BstYI RGATCY 1 cut(s) 1143
BsuRI GGCC 3 cut(s) 552, 876, 1098
BtgI CCRYGG 1 cut(s) 1008
BtsCI GGATG 1 cut(s) 733
BtsI GCAGTG 2 cut(s) 122, 995
BtsIMutI CAGTG 5 cut(s) 122, 167, 612, 810, 995
Cac8I GCNNGC 3 cut(s) 136, 554, 1100
CaiI CAGNNNCTG 1 cut(s) 997
CfoI GCGC 2 cut(s) 21, 51
Cfr13I GGNCC 1 cut(s) 1096
Csp6I GTAC 6 cut(s) 227, 355, 393, 692, 818, 1050
CviAII CATG 3 cut(s) 456, 583, 1009
CviQI GTAC 6 cut(s) 227, 355, 393, 692, 818, 1050
DdeI CTNAG 3 cut(s) 151, 547, 615
DinI GGCGCC 1 cut(s) 50
DpnI GATC 5 cut(s) 183, 747, 792, 990, 1145
DpnII GATC 5 cut(s) 181, 745, 790, 988, 1143
Eam1104I CTCTTC 1 cut(s) 584
EarI CTCTTC 1 cut(s) 584
Ecl136II GAGCTC 1 cut(s) 903
Eco130I CCWWGG 1 cut(s) 1008
Eco24I GRGCYC 1 cut(s) 905
Eco53kI GAGCTC 1 cut(s) 903
EcoICRI GAGCTC 1 cut(s) 903
EcoNI CCTNNNNNAGG 1 cut(s) 407
EcoRII CCWGG 6 cut(s) 83, 408, 736, 835, 1045, 1075
EcoT14I CCWWGG 1 cut(s) 1008
EcoT38I GRGCYC 1 cut(s) 905
EgeI GGCGCC 1 cut(s) 50
EheI GGCGCC 1 cut(s) 50
ErhI CCWWGG 1 cut(s) 1008
FaeI CATG 3 cut(s) 459, 586, 1012
FaqI GGGAC 1 cut(s) 19
FatI CATG 3 cut(s) 455, 582, 1008
FbaI TGATCA 2 cut(s) 745, 790
FblI GTMKAC 2 cut(s) 118, 1086
Fnu4HI GCNGC 3 cut(s) 604, 923, 1167
FokI GGATG 1 cut(s) 720
FriOI GRGCYC 1 cut(s) 905
Fsp4HI GCNGC 3 cut(s) 604, 923, 1167
FspBI CTAG 3 cut(s) 684, 783, 966
GlaI GCGC 2 cut(s) 20, 50
GluI GCNGC 3 cut(s) 604, 923, 1167
GsaI CCCAGC 1 cut(s) 911
HaeII RGCGCY 2 cut(s) 22, 52
HaeIII GGCC 3 cut(s) 552, 876, 1098
HapII CCGG 1 cut(s) 1230
HhaI GCGC 2 cut(s) 21, 51
Hin1I GRCGYC 1 cut(s) 49
Hin1II CATG 3 cut(s) 459, 586, 1012
Hin6I GCGC 2 cut(s) 19, 49
HinP1I GCGC 2 cut(s) 19, 49
HincII GTYRAC 2 cut(s) 103, 1116
HindII GTYRAC 2 cut(s) 103, 1116
HindIII AAGCTT 1 cut(s) 419
HinfI GANTC 3 cut(s) 34, 345, 832
HpaII CCGG 1 cut(s) 1230
HphI GGTGA 1 cut(s) 539
Hpy166II GTNNAC 6 cut(s) 103, 119, 229, 366, 1087, 1116
Hpy188I TCNGA 5 cut(s) 33, 475, 571, 676, 862
Hpy188III TCNNGA 3 cut(s) 185, 197, 487
Hpy8I GTNNAC 6 cut(s) 103, 119, 229, 366, 1087, 1116
Hpy99I CGWCG 1 cut(s) 47
HpyAV CCTTC 3 cut(s) 91, 716, 1236
HpyCH4IV ACGT 1 cut(s) 439
HpyCH4V TGCA 6 cut(s) 127, 455, 1000, 1151, 1158, 1189
HpyF10VI GCNNNNNNNGC 4 cut(s) 48, 452, 609, 943
HpyF3I CTNAG 3 cut(s) 151, 547, 615
HpySE526I ACGT 1 cut(s) 439
Hsp92I GRCGYC 1 cut(s) 49
Hsp92II CATG 3 cut(s) 459, 586, 1012
HspAI GCGC 2 cut(s) 19, 49
KasI GGCGCC 1 cut(s) 48
Ksp22I TGATCA 2 cut(s) 745, 790
Kzo9I GATC 5 cut(s) 181, 745, 790, 988, 1143
LmnI GCTCC 3 cut(s) 62, 475, 908
Lsp1109I GCAGC 3 cut(s) 615, 934, 1178
LweI GCATC 3 cut(s) 44, 934, 1219
MaeI CTAG 3 cut(s) 684, 783, 966
MaeII ACGT 1 cut(s) 439
MaeIII GTNAC 3 cut(s) 146, 215, 425
MalI GATC 5 cut(s) 183, 747, 792, 990, 1145
MboI GATC 5 cut(s) 181, 745, 790, 988, 1143
MboII GAAGA 4 cut(s) 573, 601, 815, 920
MfeI CAATTG 1 cut(s) 508
MflI RGATCY 1 cut(s) 1143
MhlI GDGCHC 4 cut(s) 284, 480, 614, 905
MluCI AATT 9 cut(s) 11, 200, 208, 382, 508, 704, 762, 1038, 1170
Mly113I GGCGCC 1 cut(s) 49
MmeI TCCRAC 2 cut(s) 453, 549
MseI TTAA 3 cut(s) 629, 1104, 1140
MslI CAYNNNNRTG 1 cut(s) 1182
MspI CCGG 1 cut(s) 1230
MspR9I CCNGG 6 cut(s) 85, 410, 738, 837, 1047, 1077
MunI CAATTG 1 cut(s) 508
MvaI CCWGG 6 cut(s) 85, 410, 738, 837, 1047, 1077
MwoI GCNNNNNNNGC 4 cut(s) 48, 452, 609, 943
NarI GGCGCC 1 cut(s) 49
NcoI CCATGG 1 cut(s) 1008
NdeII GATC 5 cut(s) 181, 745, 790, 988, 1143
NlaIII CATG 3 cut(s) 459, 586, 1012
NlaIV GGNNCC 1 cut(s) 50
NmeAIII GCCGAG 1 cut(s) 212
NmuCI GTSAC 1 cut(s) 146
NspI RCATGY 1 cut(s) 586
NspV TTCGAA 1 cut(s) 720
PciI ACATGT 1 cut(s) 582
PfeI GAWTC 3 cut(s) 34, 345, 832
PfoI TCCNGGA 1 cut(s) 736
PkrI GCNGC 3 cut(s) 605, 924, 1168
PluTI GGCGCC 1 cut(s) 52
PscI ACATGT 1 cut(s) 582
PsiI TTATAA 1 cut(s) 387
Psp124BI GAGCTC 1 cut(s) 905
Psp6I CCWGG 6 cut(s) 83, 408, 736, 835, 1045, 1075
PspFI CCCAGC 1 cut(s) 907
PspGI CCWGG 6 cut(s) 83, 408, 736, 835, 1045, 1075
PspN4I GGNNCC 1 cut(s) 50
PspPI GGNCC 1 cut(s) 1096
PstNI CAGNNNCTG 1 cut(s) 997
PsuI RGATCY 1 cut(s) 1143
RsaI GTAC 6 cut(s) 228, 356, 394, 693, 819, 1051
RsaNI GTAC 6 cut(s) 227, 355, 393, 692, 818, 1050
RseI CAYNNNNRTG 1 cut(s) 1182
SacI GAGCTC 1 cut(s) 905
SaqAI TTAA 3 cut(s) 629, 1104, 1140
SatI GCNGC 3 cut(s) 604, 923, 1167
Sau3AI GATC 5 cut(s) 181, 745, 790, 988, 1143
Sau96I GGNCC 1 cut(s) 1096
ScrFI CCNGG 6 cut(s) 85, 410, 738, 837, 1047, 1077
SduI GDGCHC 4 cut(s) 284, 480, 614, 905
SfaNI GCATC 3 cut(s) 44, 934, 1219
SfcI CTRYAG 2 cut(s) 495, 813
SfoI GGCGCC 1 cut(s) 50
SfuI TTCGAA 1 cut(s) 720
SmiMI CAYNNNNRTG 1 cut(s) 1182
SmlI CTYRAG 2 cut(s) 263, 695
SmoI CTYRAG 2 cut(s) 263, 695
Sse9I AATT 9 cut(s) 11, 200, 208, 382, 508, 704, 762, 1038, 1170
SspDI GGCGCC 1 cut(s) 48
SspMI CTAG 3 cut(s) 684, 783, 966
SstI GAGCTC 1 cut(s) 905
StyD4I CCNGG 6 cut(s) 83, 408, 736, 835, 1045, 1075
StyI CCWWGG 1 cut(s) 1008
TaiI ACGT 1 cut(s) 442
TaqI TCGA 2 cut(s) 720, 879
TasI AATT 9 cut(s) 11, 200, 208, 382, 508, 704, 762, 1038, 1170
TatI WGTACW 3 cut(s) 226, 691, 817
TfiI GAWTC 3 cut(s) 34, 345, 832
Tru1I TTAA 3 cut(s) 629, 1104, 1140
Tru9I TTAA 3 cut(s) 629, 1104, 1140
TscAI CASTG 5 cut(s) 129, 174, 619, 817, 1002
TseFI GTSAC 1 cut(s) 146
TseI GCWGC 3 cut(s) 603, 922, 1166
Tsp45I GTSAC 1 cut(s) 146
TspDTI ATGAA 6 cut(s) 257, 320, 845, 869, 1166, 1206
TspRI CASTG 5 cut(s) 129, 174, 619, 817, 1002
XagI CCTNNNNNAGG 1 cut(s) 407
XapI RAATTY 3 cut(s) 11, 200, 704
XceI RCATGY 1 cut(s) 586
XmiI GTMKAC 2 cut(s) 118, 1086
XspI CTAG 3 cut(s) 684, 783, 966
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.