Rw2G033340

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001058
Physical Location & Seq
Reverse (-)
54622811 .. 54624462
1652 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G033340.1

Sequence Viewer

Length: 1305 bp
ATGCAAACTGCTTTTTACATAGCCTGCAGTTATGGACATGTTGATGTTGTCAAGTACATGGCACACCAACCAGGGATGACTATTTTGAAAGGTTGCCCCAGGTTTGCTTGGAAGGTTGACAGCAATGGGTGTCTACCACTGCATATAGCTTGCGAAAAAGGTCACTTAGAGATAGCAAGAACACTGTTGATGATTGATCCAGACCTTGCTCTTGAATTTGACAATTACCGTTACACACCAGTACACTCGGCTGTAATGAATGGTCAAATCAAAATCCTTGAGGAACTCCTTTTGTGCTCTCCCAAATGTTTAGCAAGGCTCACAGGAGATGGAGAAACTGTCTTTCATCTTTCAGTAAGATTCAATAGGTACGGTGTGTTTACCTCTTTGGTAAAGAATTATAACAGTACCTATCTCCTCAACCAGGCTGACAAGCTTGGTAACACAATCTTACATCTTGCTGTCTCTGCATGGAACATTACATTGTCGGAGCACATCATCAGGAATACTACAGTTGATGTCAATTGCCAGCAAAGAAGAATGTCGGATATAAGAAACATGTGGAAGAGTTTTGGTGGCAGCAAGAGCACTGATCTGTTGTCTTTAACACCAAAGATTGATGAGATAGATAATGAGACTACAACAACATCAGAAAGTCCTAGAGTTGTACTTGAGCAAGAATTTGAAATGCTTTTCGAAGGTATCACACATCCAGGAAGTGATCAATATGATTGTGAAATTGGTATGGGGCGTATAGCTAGAAATGATCAACCAATGGAAGATTTCACTGTAGTACAAGAAAAGATGAATCCAGGTCATCAAGTTGTGATGAACTCTGACAATCTTTTTGGCCTCGATTGTTTTGAAACAAGAATGAGCTCCCCAGCCTTGAATGAGCAGCATCTTCCAAAGCCAAGAGGGCAAAACAAAGTGGGAAAAACTAGGCAATATGAGATATATAGAGAGGCACTGCAAAATACCATGGACACAATAACATTAGTTGCCATTCTAATTGCCCCAGGTACTTTCACTACTGGTATATACCCTCCTGGTGGTGTCTACGAGGAGGGCCAGCTTAAAGGACAGTCAACAGTTGGCAGAACAACAGCTTTTAAGATCTTTGCAATATCATCATTCATCATTCCCTTCCGGCGAAAACCACTAATGAAACTACTGGTGGTTGCTCACAAAGTAATGTGGGTGGCGGTGTCACTGATGCCACACGGTGAAGGTGTAAATTGGACCCTAGAAGTAGTGATTTCTATGTGTGCAGGGACTATGGACCATGGACCAGACACTGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

434

Amino Acids

48.62

Weight (kDa)

6.17

Isoelectric Point (pI)

32.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 2 - 67 7.4e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 39 - 97 3.7e-10 Ankyrin repeats (3 copies)
Ank_2 PF12796 82 - 175 1.9e-06 Ankyrin repeats (3 copies)
PGG PF13962 322 - 379 1e-09 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 402
AccB7I CCANNNNNTGG 1 cut(s) 1298
AccI GTMKAC 2 cut(s) 133, 1059
AciI CCGC 1 cut(s) 1205
AclWI GGATC 1 cut(s) 191
AcsI RAATTY 2 cut(s) 215, 680
AdeI CACNNNGTG 1 cut(s) 1226
AfaI GTAC 7 cut(s) 56, 243, 371, 409, 669, 795, 1024
AfiI CCNNNNNNNGG 3 cut(s) 424, 1052, 1298
AflIII ACRYGT 2 cut(s) 37, 558
AgsI TTSAA 6 cut(s) 88, 215, 364, 686, 866, 892
AjnI CCWGG 7 cut(s) 70, 98, 423, 712, 811, 1018, 1048
AluBI AGCT 6 cut(s) 149, 436, 758, 879, 1075, 1109
AluI AGCT 6 cut(s) 149, 436, 758, 879, 1075, 1109
Alw21I GWGCWC 4 cut(s) 299, 495, 590, 881
Alw26I GTCTC 2 cut(s) 469, 629
AlwI GGATC 1 cut(s) 191
AlwNI CAGNNNCTG 1 cut(s) 1298
AoxI GGCC 2 cut(s) 850, 1069
ApeKI GCWGC 2 cut(s) 579, 898
ApoI RAATTY 2 cut(s) 215, 680
ArsI GACNNNNNNTTYG 2 cut(s) 830, 862
AspS9I GGNCC 4 cut(s) 1069, 1242, 1282, 1289
AsuHPI GGTGA 1 cut(s) 1238
AsuII TTCGAA 1 cut(s) 696
AvaII GGWCC 3 cut(s) 1242, 1282, 1289
BanII GRGCYC 1 cut(s) 881
Bbv12I GWGCWC 4 cut(s) 299, 495, 590, 881
BbvI GCAGC 2 cut(s) 591, 910
BccI CCATC 1 cut(s) 323
BciT130I CCWGG 7 cut(s) 72, 100, 425, 714, 813, 1020, 1050
BclI TGATCA 2 cut(s) 721, 766
BcoDI GTCTC 2 cut(s) 469, 629
BfaI CTAG 4 cut(s) 660, 759, 942, 1247
BfmI CTRYAG 3 cut(s) 25, 510, 789
BglI GCCNNNNNGGC 1 cut(s) 919
BglII AGATCT 1 cut(s) 1116
BisI GCNGC 2 cut(s) 580, 899
BlsI GCNGC 2 cut(s) 581, 900
Bme1390I CCNGG 7 cut(s) 72, 100, 425, 714, 813, 1020, 1050
Bme18I GGWCC 3 cut(s) 1242, 1282, 1289
BmgT120I GGNCC 4 cut(s) 1069, 1242, 1282, 1289
BmiI GGNNCC 1 cut(s) 1244
BmrFI CCNGG 7 cut(s) 72, 100, 425, 714, 813, 1020, 1050
BmsI GCATC 2 cut(s) 910, 1206
Bpu14I TTCGAA 1 cut(s) 696
BpuEI CTTGAG 2 cut(s) 299, 692
BsaJI CCNNGG 5 cut(s) 71, 98, 981, 1018, 1285
BsaXI ACNNNNNCTCC 2 cut(s) 324, 354
Bsc4I CCNNNNNNNGG 3 cut(s) 424, 1052, 1298
Bse1I ACTGG 4 cut(s) 239, 1039, 1179, 1303
Bse3DI GCAATG 1 cut(s) 130
BseBI CCWGG 7 cut(s) 72, 100, 425, 714, 813, 1020, 1050
BseDI CCNNGG 5 cut(s) 71, 98, 981, 1018, 1285
BseGI GGATG 2 cut(s) 81, 709
BseLI CCNNNNNNNGG 3 cut(s) 424, 1052, 1298
BseMI GCAATG 1 cut(s) 130
BseNI ACTGG 4 cut(s) 239, 1039, 1179, 1303
BseRI GAGGAG 2 cut(s) 407, 1079
BseXI GCAGC 2 cut(s) 591, 910
BseYI CCCAGC 1 cut(s) 883
BsgI GTGCAG 1 cut(s) 1290
BshFI GGCC 2 cut(s) 852, 1071
BsiHKAI GWGCWC 4 cut(s) 299, 495, 590, 881
BsiSI CCGG 1 cut(s) 1150
BslFI GGGAC 1 cut(s) 1288
BslI CCNNNNNNNGG 3 cut(s) 424, 1052, 1298
BsmAI GTCTC 2 cut(s) 469, 629
BsmFI GGGAC 1 cut(s) 1288
BsnI GGCC 2 cut(s) 852, 1071
Bsp119I TTCGAA 1 cut(s) 696
Bsp1286I GDGCHC 4 cut(s) 299, 495, 590, 881
Bsp143I GATC 5 cut(s) 196, 592, 721, 766, 1116
Bsp19I CCATGG 2 cut(s) 981, 1285
BspACI CCGC 1 cut(s) 1205
BspANI GGCC 2 cut(s) 852, 1071
BspLI GGNNCC 1 cut(s) 1244
BspMAI CTGCAG 1 cut(s) 29
BspPI GGATC 1 cut(s) 191
BspT104I TTCGAA 1 cut(s) 696
BsrDI GCAATG 1 cut(s) 130
BsrI ACTGG 4 cut(s) 239, 1039, 1179, 1303
BssECI CCNNGG 5 cut(s) 71, 98, 981, 1018, 1285
BssMI GATC 5 cut(s) 196, 592, 721, 766, 1116
BssT1I CCWWGG 2 cut(s) 981, 1285
Bst2UI CCWGG 7 cut(s) 72, 100, 425, 714, 813, 1020, 1050
Bst6I CTCTTC 1 cut(s) 560
BstBI TTCGAA 1 cut(s) 696
BstC8I GCNNGC 4 cut(s) 25, 151, 530, 1073
BstDEI CTNAG 1 cut(s) 166
BstDSI CCRYGG 2 cut(s) 981, 1285
BstENI CCTNNNNNAGG 1 cut(s) 422
BstF5I GGATG 2 cut(s) 81, 709
BstKTI GATC 5 cut(s) 199, 595, 724, 769, 1119
BstMAI GTCTC 2 cut(s) 469, 629
BstMBI GATC 5 cut(s) 196, 592, 721, 766, 1116
BstMWI GCNNNNNNNGC 3 cut(s) 467, 585, 919
BstNI CCWGG 7 cut(s) 72, 100, 425, 714, 813, 1020, 1050
BstNSI RCATGY 2 cut(s) 41, 562
BstSCI CCNGG 7 cut(s) 70, 98, 423, 712, 811, 1018, 1048
BstSFI CTRYAG 3 cut(s) 25, 510, 789
BstV1I GCAGC 2 cut(s) 591, 910
BstX2I RGATCY 1 cut(s) 1116
BstYI RGATCY 1 cut(s) 1116
BsuRI GGCC 2 cut(s) 852, 1071
BtgI CCRYGG 2 cut(s) 981, 1285
BtsCI GGATG 2 cut(s) 81, 709
BtsI GCAGTG 2 cut(s) 137, 968
BtsIMutI CAGTG 7 cut(s) 137, 182, 588, 786, 968, 1211, 1296
Cac8I GCNNGC 4 cut(s) 25, 151, 530, 1073
CaiI CAGNNNCTG 1 cut(s) 1298
Cfr13I GGNCC 4 cut(s) 1069, 1242, 1282, 1289
Csp6I GTAC 7 cut(s) 55, 242, 370, 408, 668, 794, 1023
CviAII CATG 6 cut(s) 38, 58, 471, 559, 982, 1286
CviQI GTAC 7 cut(s) 55, 242, 370, 408, 668, 794, 1023
DdeI CTNAG 1 cut(s) 166
DpnI GATC 5 cut(s) 198, 594, 723, 768, 1118
DpnII GATC 5 cut(s) 196, 592, 721, 766, 1116
DraIII CACNNNGTG 1 cut(s) 1226
Eam1104I CTCTTC 1 cut(s) 560
EarI CTCTTC 1 cut(s) 560
Ecl136II GAGCTC 1 cut(s) 879
Eco130I CCWWGG 2 cut(s) 981, 1285
Eco24I GRGCYC 1 cut(s) 881
Eco47I GGWCC 3 cut(s) 1242, 1282, 1289
Eco53kI GAGCTC 1 cut(s) 879
EcoICRI GAGCTC 1 cut(s) 879
EcoNI CCTNNNNNAGG 1 cut(s) 422
EcoRII CCWGG 7 cut(s) 70, 98, 423, 712, 811, 1018, 1048
EcoT14I CCWWGG 2 cut(s) 981, 1285
EcoT38I GRGCYC 1 cut(s) 881
ErhI CCWWGG 2 cut(s) 981, 1285
FaeI CATG 6 cut(s) 41, 61, 474, 562, 985, 1289
FaqI GGGAC 1 cut(s) 1288
FatI CATG 6 cut(s) 37, 57, 470, 558, 981, 1285
FbaI TGATCA 2 cut(s) 721, 766
FblI GTMKAC 2 cut(s) 133, 1059
Fnu4HI GCNGC 2 cut(s) 580, 899
FokI GGATG 2 cut(s) 88, 696
FriOI GRGCYC 1 cut(s) 881
Fsp4HI GCNGC 2 cut(s) 580, 899
FspBI CTAG 4 cut(s) 660, 759, 942, 1247
GluI GCNGC 2 cut(s) 580, 899
GsaI CCCAGC 1 cut(s) 887
HaeIII GGCC 2 cut(s) 852, 1071
HapII CCGG 1 cut(s) 1150
Hin1II CATG 6 cut(s) 41, 61, 474, 562, 985, 1289
HincII GTYRAC 2 cut(s) 118, 1089
HindII GTYRAC 2 cut(s) 118, 1089
HindIII AAGCTT 1 cut(s) 434
HinfI GANTC 2 cut(s) 360, 808
HpaII CCGG 1 cut(s) 1150
HphI GGTGA 1 cut(s) 1238
Hpy166II GTNNAC 6 cut(s) 118, 134, 244, 381, 1060, 1089
Hpy188I TCNGA 4 cut(s) 490, 547, 652, 838
Hpy188III TCNNGA 3 cut(s) 200, 212, 502
Hpy8I GTNNAC 6 cut(s) 118, 134, 244, 381, 1060, 1089
HpyAV CCTTC 4 cut(s) 106, 692, 1156, 1223
HpyCH4V TGCA 7 cut(s) 4, 27, 142, 470, 973, 1124, 1271
HpyF10VI GCNNNNNNNGC 3 cut(s) 467, 585, 919
HpyF3I CTNAG 1 cut(s) 166
Hsp92II CATG 6 cut(s) 41, 61, 474, 562, 985, 1289
Ksp22I TGATCA 2 cut(s) 721, 766
Kzo9I GATC 5 cut(s) 196, 592, 721, 766, 1116
LmnI GCTCC 2 cut(s) 490, 884
Lsp1109I GCAGC 2 cut(s) 591, 910
LweI GCATC 2 cut(s) 910, 1206
MaeI CTAG 4 cut(s) 660, 759, 942, 1247
MaeIII GTNAC 4 cut(s) 161, 230, 440, 1209
MalI GATC 5 cut(s) 198, 594, 723, 768, 1118
MboI GATC 5 cut(s) 196, 592, 721, 766, 1116
MboII GAAGA 4 cut(s) 549, 577, 791, 896
MfeI CAATTG 1 cut(s) 523
MflI RGATCY 1 cut(s) 1116
MhlI GDGCHC 4 cut(s) 299, 495, 590, 881
MluCI AATT 8 cut(s) 215, 223, 397, 523, 680, 738, 1011, 1237
MmeI TCCRAC 2 cut(s) 468, 525
MnlI CCTC 9 cut(s) 274, 394, 428, 863, 911, 958, 1056, 1057, 1060
MseI TTAA 3 cut(s) 605, 1077, 1113
MslI CAYNNNNRTG 1 cut(s) 42
MspI CCGG 1 cut(s) 1150
MspR9I CCNGG 7 cut(s) 72, 100, 425, 714, 813, 1020, 1050
MunI CAATTG 1 cut(s) 523
MvaI CCWGG 7 cut(s) 72, 100, 425, 714, 813, 1020, 1050
MwoI GCNNNNNNNGC 3 cut(s) 467, 585, 919
NcoI CCATGG 2 cut(s) 981, 1285
NdeII GATC 5 cut(s) 196, 592, 721, 766, 1116
NlaIII CATG 6 cut(s) 41, 61, 474, 562, 985, 1289
NlaIV GGNNCC 1 cut(s) 1244
NmeAIII GCCGAG 1 cut(s) 227
NmuCI GTSAC 2 cut(s) 161, 1209
NspI RCATGY 2 cut(s) 41, 562
NspV TTCGAA 1 cut(s) 696
PciI ACATGT 2 cut(s) 37, 558
PfeI GAWTC 2 cut(s) 360, 808
PflMI CCANNNNNTGG 1 cut(s) 1298
PfoI TCCNGGA 1 cut(s) 712
PkrI GCNGC 2 cut(s) 581, 900
PscI ACATGT 2 cut(s) 37, 558
PsiI TTATAA 1 cut(s) 402
Psp124BI GAGCTC 1 cut(s) 881
Psp6I CCWGG 7 cut(s) 70, 98, 423, 712, 811, 1018, 1048
PspFI CCCAGC 1 cut(s) 883
PspGI CCWGG 7 cut(s) 70, 98, 423, 712, 811, 1018, 1048
PspN4I GGNNCC 1 cut(s) 1244
PspPI GGNCC 4 cut(s) 1069, 1242, 1282, 1289
PstI CTGCAG 1 cut(s) 29
PstNI CAGNNNCTG 1 cut(s) 1298
PsuI RGATCY 1 cut(s) 1116
RsaI GTAC 7 cut(s) 56, 243, 371, 409, 669, 795, 1024
RsaNI GTAC 7 cut(s) 55, 242, 370, 408, 668, 794, 1023
RseI CAYNNNNRTG 1 cut(s) 42
SacI GAGCTC 1 cut(s) 881
SaqAI TTAA 3 cut(s) 605, 1077, 1113
SatI GCNGC 2 cut(s) 580, 899
Sau3AI GATC 5 cut(s) 196, 592, 721, 766, 1116
Sau96I GGNCC 4 cut(s) 1069, 1242, 1282, 1289
ScrFI CCNGG 7 cut(s) 72, 100, 425, 714, 813, 1020, 1050
SduI GDGCHC 4 cut(s) 299, 495, 590, 881
SfaNI GCATC 2 cut(s) 910, 1206
SfcI CTRYAG 3 cut(s) 25, 510, 789
SfuI TTCGAA 1 cut(s) 696
SinI GGWCC 3 cut(s) 1242, 1282, 1289
SmiMI CAYNNNNRTG 1 cut(s) 42
SmlI CTYRAG 2 cut(s) 278, 671
SmoI CTYRAG 2 cut(s) 278, 671
Sse9I AATT 8 cut(s) 215, 223, 397, 523, 680, 738, 1011, 1237
SsiI CCGC 1 cut(s) 1205
SspMI CTAG 4 cut(s) 660, 759, 942, 1247
SstI GAGCTC 1 cut(s) 881
StyD4I CCNGG 7 cut(s) 70, 98, 423, 712, 811, 1018, 1048
StyI CCWWGG 2 cut(s) 981, 1285
TaqI TCGA 2 cut(s) 696, 855
TasI AATT 8 cut(s) 215, 223, 397, 523, 680, 738, 1011, 1237
TatI WGTACW 4 cut(s) 54, 241, 667, 793
TfiI GAWTC 2 cut(s) 360, 808
Tru1I TTAA 3 cut(s) 605, 1077, 1113
Tru9I TTAA 3 cut(s) 605, 1077, 1113
TscAI CASTG 7 cut(s) 144, 189, 595, 793, 975, 1218, 1303
TseFI GTSAC 2 cut(s) 161, 1209
TseI GCWGC 2 cut(s) 579, 898
Tsp45I GTSAC 2 cut(s) 161, 1209
TspDTI ATGAA 6 cut(s) 272, 335, 821, 845, 1126, 1181
TspRI CASTG 7 cut(s) 144, 189, 595, 793, 975, 1218, 1303
Van91I CCANNNNNTGG 1 cut(s) 1298
VpaK11BI GGWCC 3 cut(s) 1242, 1282, 1289
XagI CCTNNNNNAGG 1 cut(s) 422
XapI RAATTY 2 cut(s) 215, 680
XceI RCATGY 2 cut(s) 41, 562
XmiI GTMKAC 2 cut(s) 133, 1059
XspI CTAG 4 cut(s) 660, 759, 942, 1247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.