Prupe.3G062200_v2.0.a1

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0001058
Physical Location & Seq
Reverse (-)
4433404 .. 4435975
2572 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G062200.1

Sequence Viewer

Length: 2013 bp
ATGCATACAGGGCTTTTTGAGGCAATTGCAGCAAATGACAAGCAAACTTTCATCAATCTGGTTCAAGACAATGAAGGATTTCTCAGTCAAAAAGAGGGTGAAGCCAAAAACAGTGTGTTACACCTTGCTGTGAGGTTCGGGCACGTTGAGCTGGTTTCTGAAATCATTACGCTCTTCCCGGACTTGGTTGCAGTTGTGAACAATAGAGAAGAAACCCCTTTACATGCCGCTTGTCGGCAAGGGAAAGTCGATGTTGTAATGCTGCTGTTAGAGGCCAATCCTGGGGTGTCTTCTTGTATGCTTAATTCTGAAAATCAAACCCCCTTCTTCATAGCTTGCAGTTATGGGAATCGTGAAGTGGTGAAGCTGTTCACAGACCTACCATGGGTACTAGATTTTGAAGAAGATGCGGTTTTGAGTGCTCTTCATGTGTCCGTAGCACAAGGCCATGCGGATATTGTTACAACACTTTTGAATGCACGACCAAGGCTTGCTCGGAAGGCTGACAGCAATGGATGTTTACCATTGCATTTGGCTTGTGAGAAAGGTCACTTGGAGATAACAAGAATACTCTTACAGCTTGATCCAAACCTTGCTTTTGAAAAGAATTATTCTCGTTACACGCCGTTACACTTTGCAGCAATGAATGGACAAACCCAAATCCTTGAGGAACTTTTGTCCAGGTCTCCCAAATCTTTCGAAGTTCTCACCGGAGAAGGAGAAACAGTGTTTCATATTGCTGTCATGTTCAACAGATACAGTTCCTTTGTCTTTTTGGCACAATCTTTTAACAGCACCAATCTCCTCCACCTTCCAGATCAACGTGGTAACACCGTCTTACATCTAGCTGTCACTACAAGCAGCTTGAAATTAGCAGAGTACATCATTGAGAAAACCGAGGTGGATATCAATCGTCGGAACCATAGAGGTCTAACAGCCTATGACATCCTCAACAGGAACTATAGCATGACGGAGAACCAGCATGTTAGGGACATGTTGAAGAGATTTGGTGGCACTGGAAAAACGGCACAAGAATTACAAACGCCTATTGAATTTAATCCGCACCAAAGTGAACACAATTACGGAAATGGTGTAGGCAACAACCAACCAGACAGTAATCTGAGTCATCAAGCCACAATAAACTTTGACAAACAGATAGATAGTCCTTCTGCACCACCAGCCAATTTGCATCAATCCAAGCTTGTGAAGCAAAGAGGGAAGAAAAAAATAAGTACAGTTAGGCAATATGAGATATATAGAGAGGCCCTACAAAATACCAGGAACACAATCACACTAGTTGCCATTTTGATTGCATCGGTTACTTTCACCGCTGGCATAAACCCTCCCGGGGGTGTGTACCAGGAAGGACTACTAAAAGGACAGTCAACAATTGGAAGAACAACTGCTTTCAAGGTCTTCACAATAAGCAACACAGCTGCGTTGTTCTTATCTATGTGCATAGTCATTGTCTTAGTGAGCATCATTCCCTTCCGGCGTAAAACACTGATGAAGCTACTGGTGGTTGCTCACAAAGCCATGTGGGTGGCAGTGTCACTCATGACCACAGCTTACATTGCAGCCACATGGGTGATCTTGCCACACAGTCAAGGAACAAATTGGACCCTTGAGGCTCTCATATCTGTATGTGCAGGGACTATGGGATCTGTTTTTCTATTTCTTTGTGTCACATTTGCTAGACACTGGTTGAGGAAGTTACAGTGGAGGAAAGAGAAAGGTAAGGTGAAAGAGGAAAGAGTGGAGGAAAGAGAGCAAAGAAGAGCACAGAGGAGGGCAGAGAAGACAGCAAAGGAAAGAGTGCAGAAAAGAGAAAGTGCGGAGAAGAGAGCAAAGGAAAGTAGACAGGAAAGTAGACTGGCAAGTAGACAGGAAAGTTCACAGGATGTTAGTATAAAGGTATTTTCACTGGAAAGTTCACAGGAAGGATCAGAAGATTTAATTTCCATAAACTCAGACACTGCCAGTGCTCAAGATTCGGGATATCATACGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 PGG

Protein Analysis

671

Amino Acids

75.08

Weight (kDa)

8.71

Isoelectric Point (pI)

40.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 1858, 1870, 1882
AciI CCGC 6 cut(s) 228, 410, 452, 1061, 1329, 1835
AclWI GGATC 3 cut(s) 578, 1669, 1951
AcsI RAATTY 1 cut(s) 1052
AfaI GTAC 4 cut(s) 390, 881, 1234, 1358
AfiI CCNNNNNNNGG 6 cut(s) 184, 234, 282, 385, 1348, 1349
AflIII ACRYGT 1 cut(s) 993
AgsI TTSAA 9 cut(s) 65, 401, 475, 602, 751, 868, 1000, 1052, 1411
AhlI ACTAGT 1 cut(s) 1294
AjnI CCWGG 4 cut(s) 280, 680, 1277, 1359
AjuI GAANNNNNNNTTGG 2 cut(s) 536, 568
AleI CACNNNNGTG 2 cut(s) 1068, 1586
Alw21I GWGCWC 3 cut(s) 424, 1783, 1987
Alw26I GTCTC 1 cut(s) 690
AlwI GGATC 3 cut(s) 578, 1669, 1951
AlwNI CAGNNNCTG 1 cut(s) 1976
Ama87I CYCGRG 1 cut(s) 1346
AoxI GGCC 3 cut(s) 273, 445, 1263
ApeKI GCWGC 6 cut(s) 29, 262, 638, 861, 1436, 1577
ApoI RAATTY 1 cut(s) 1052
Asp700I GAANNNNTTC 2 cut(s) 78, 368
AspS9I GGNCC 2 cut(s) 1264, 1620
AsuC2I CCSGG 3 cut(s) 179, 1347, 1348
AsuHPI GGTGA 6 cut(s) 110, 373, 700, 1318, 1600, 1753
AsuII TTCGAA 1 cut(s) 699
AvaI CYCGRG 1 cut(s) 1346
AvaII GGWCC 1 cut(s) 1620
BaeGI GKGCMC 1 cut(s) 144
BbsI GAAGAC 3 cut(s) 282, 1408, 1805
Bbv12I GWGCWC 3 cut(s) 424, 1783, 1987
BbvI GCAGC 6 cut(s) 41, 249, 650, 873, 1423, 1589
BceAI ACGGC 2 cut(s) 610, 1041
BciT130I CCWGG 4 cut(s) 282, 682, 1279, 1361
BcnI CCSGG 3 cut(s) 179, 1347, 1348
BcoDI GTCTC 1 cut(s) 690
BcuI ACTAGT 1 cut(s) 1294
BfaI CTAG 4 cut(s) 392, 845, 1295, 1695
BfmI CTRYAG 1 cut(s) 961
BisI GCNGC 7 cut(s) 30, 228, 263, 639, 862, 1437, 1578
BlsI GCNGC 7 cut(s) 31, 229, 264, 640, 863, 1438, 1579
Bme1390I CCNGG 7 cut(s) 179, 282, 682, 1279, 1347, 1348, 1361
Bme18I GGWCC 1 cut(s) 1620
BmeT110I CYCGRG 1 cut(s) 1346
BmgT120I GGNCC 2 cut(s) 1264, 1620
BmiI GGNNCC 2 cut(s) 920, 1622
BmrFI CCNGG 7 cut(s) 179, 282, 682, 1279, 1347, 1348, 1361
BmsI GCATC 4 cut(s) 397, 1198, 1322, 1488
BpiI GAAGAC 3 cut(s) 282, 1408, 1805
Bpu14I TTCGAA 1 cut(s) 699
BpuEI CTTGAG 3 cut(s) 686, 1646, 1971
BpuMI CCSGG 3 cut(s) 179, 1347, 1348
BsaBI GATNNNNATC 1 cut(s) 909
BsaI GGTCTC 1 cut(s) 690
BsaJI CCNNGG 6 cut(s) 281, 383, 485, 897, 1346, 1347
BsaWI WCCGGW 1 cut(s) 710
BsaXI ACNNNNNCTCC 2 cut(s) 711, 741
Bsc4I CCNNNNNNNGG 6 cut(s) 184, 234, 282, 385, 1348, 1349
Bse1I ACTGG 6 cut(s) 1021, 1521, 1706, 1878, 1929, 1980
Bse3DI GCAATG 4 cut(s) 517, 524, 648, 1572
Bse8I GATNNNNATC 1 cut(s) 909
BseBI CCWGG 4 cut(s) 282, 682, 1279, 1361
BseDI CCNNGG 6 cut(s) 281, 383, 485, 897, 1346, 1347
BseGI GGATG 3 cut(s) 521, 945, 1906
BseJI GATNNNNATC 1 cut(s) 909
BseLI CCNNNNNNNGG 6 cut(s) 184, 234, 282, 385, 1348, 1349
BseMI GCAATG 4 cut(s) 517, 524, 648, 1572
BseMII CTCAG 3 cut(s) 97, 1112, 1983
BseNI ACTGG 6 cut(s) 1021, 1521, 1706, 1878, 1929, 1980
BseRI GAGGAG 2 cut(s) 794, 1801
BseSI GKGCMC 1 cut(s) 144
BseXI GCAGC 6 cut(s) 41, 249, 650, 873, 1423, 1589
BsgI GTGCAG 3 cut(s) 1155, 1668, 1838
BshFI GGCC 3 cut(s) 275, 447, 1265
BsiHKAI GWGCWC 3 cut(s) 424, 1783, 1987
BsiHKCI CYCGRG 1 cut(s) 1346
BsiSI CCGG 4 cut(s) 179, 711, 1347, 1492
BslFI GGGAC 2 cut(s) 1004, 1666
BslI CCNNNNNNNGG 6 cut(s) 184, 234, 282, 385, 1348, 1349
BsmAI GTCTC 1 cut(s) 690
BsmFI GGGAC 2 cut(s) 1004, 1666
BsmI GAATGC 1 cut(s) 481
BsnI GGCC 3 cut(s) 275, 447, 1265
Bso31I GGTCTC 1 cut(s) 690
BsoBI CYCGRG 1 cut(s) 1346
Bsp119I TTCGAA 1 cut(s) 699
Bsp1286I GDGCHC 4 cut(s) 144, 424, 1783, 1987
Bsp143I GATC 5 cut(s) 583, 817, 1590, 1661, 1943
Bsp19I CCATGG 1 cut(s) 383
BspACI CCGC 6 cut(s) 228, 410, 452, 1061, 1329, 1835
BspANI GGCC 3 cut(s) 275, 447, 1265
BspCNI CTCAG 3 cut(s) 96, 1113, 1982
BspHI TCATGA 1 cut(s) 1557
BspLI GGNNCC 2 cut(s) 920, 1622
BspPI GGATC 3 cut(s) 578, 1669, 1951
BspQI GCTCTTC 3 cut(s) 179, 429, 1771
BspT104I TTCGAA 1 cut(s) 699
BspTNI GGTCTC 1 cut(s) 690
BsrDI GCAATG 4 cut(s) 517, 524, 648, 1572
BsrI ACTGG 6 cut(s) 1021, 1521, 1706, 1878, 1929, 1980
BssECI CCNNGG 6 cut(s) 281, 383, 485, 897, 1346, 1347
BssMI GATC 5 cut(s) 583, 817, 1590, 1661, 1943
BssT1I CCWWGG 2 cut(s) 383, 485
Bst2UI CCWGG 4 cut(s) 282, 682, 1279, 1361
Bst4CI ACNGT 9 cut(s) 113, 727, 761, 835, 1115, 1237, 1383, 1604, 1719
Bst6I CTCTTC 5 cut(s) 179, 429, 995, 1771, 1835
BstBI TTCGAA 1 cut(s) 699
BstC8I GCNNGC 3 cut(s) 337, 492, 1333
BstDEI CTNAG 4 cut(s) 83, 1121, 1471, 1969
BstDSI CCRYGG 1 cut(s) 383
BstF5I GGATG 3 cut(s) 521, 945, 1906
BstKTI GATC 5 cut(s) 586, 820, 1593, 1664, 1946
BstMAI GTCTC 1 cut(s) 690
BstMBI GATC 5 cut(s) 583, 817, 1590, 1661, 1943
BstMWI GCNNNNNNNGC 8 cut(s) 10, 29, 148, 500, 1178, 1207, 1532, 1574
BstNI CCWGG 4 cut(s) 282, 682, 1279, 1361
BstNSI RCATGY 3 cut(s) 227, 986, 997
BstSCI CCNGG 7 cut(s) 177, 280, 680, 1277, 1345, 1346, 1359
BstSFI CTRYAG 1 cut(s) 961
BstSLI GKGCMC 1 cut(s) 144
BstV1I GCAGC 6 cut(s) 41, 249, 650, 873, 1423, 1589
BstV2I GAAGAC 3 cut(s) 282, 1408, 1805
BstX2I RGATCY 1 cut(s) 1661
BstXI CCANNNNNNTGG 1 cut(s) 1543
BstYI RGATCY 1 cut(s) 1661
BsuRI GGCC 3 cut(s) 275, 447, 1265
BtgI CCRYGG 1 cut(s) 383
BtsCI GGATG 3 cut(s) 521, 945, 1906
BtsI GCAGTG 2 cut(s) 1554, 1974
Cac8I GCNNGC 3 cut(s) 337, 492, 1333
CaiI CAGNNNCTG 1 cut(s) 1976
CciI TCATGA 1 cut(s) 1557
Cfr13I GGNCC 2 cut(s) 1264, 1620
Cfr9I CCCGGG 1 cut(s) 1346
Csp6I GTAC 4 cut(s) 389, 880, 1233, 1357
CviQI GTAC 4 cut(s) 389, 880, 1233, 1357
DdeI CTNAG 4 cut(s) 83, 1121, 1471, 1969
DpnI GATC 5 cut(s) 585, 819, 1592, 1663, 1945
DpnII GATC 5 cut(s) 583, 817, 1590, 1661, 1943
Eam1104I CTCTTC 5 cut(s) 179, 429, 995, 1771, 1835
EarI CTCTTC 5 cut(s) 179, 429, 995, 1771, 1835
Eco130I CCWWGG 2 cut(s) 383, 485
Eco31I GGTCTC 1 cut(s) 690
Eco32I GATATC 2 cut(s) 907, 2000
Eco47I GGWCC 1 cut(s) 1620
Eco88I CYCGRG 1 cut(s) 1346
EcoO109I RGGNCCY 1 cut(s) 1264
EcoRII CCWGG 4 cut(s) 280, 680, 1277, 1359
EcoRV GATATC 2 cut(s) 907, 2000
EcoT14I CCWWGG 2 cut(s) 383, 485
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 383, 485
FaqI GGGAC 2 cut(s) 1004, 1666
FblI GTMKAC 3 cut(s) 1858, 1870, 1882
Fnu4HI GCNGC 7 cut(s) 30, 228, 263, 639, 862, 1437, 1578
FokI GGATG 3 cut(s) 528, 932, 1913
Fsp4HI GCNGC 7 cut(s) 30, 228, 263, 639, 862, 1437, 1578
FspBI CTAG 4 cut(s) 392, 845, 1295, 1695
GluI GCNGC 7 cut(s) 30, 228, 263, 639, 862, 1437, 1578
HaeIII GGCC 3 cut(s) 275, 447, 1265
HapII CCGG 4 cut(s) 179, 711, 1347, 1492
HincII GTYRAC 1 cut(s) 1386
HindII GTYRAC 1 cut(s) 1386
HindIII AAGCTT 1 cut(s) 1199
HinfI GANTC 3 cut(s) 349, 1123, 1991
HpaII CCGG 4 cut(s) 179, 711, 1347, 1492
HphI GGTGA 6 cut(s) 110, 373, 700, 1318, 1600, 1753
Hpy188I TCNGA 8 cut(s) 160, 310, 498, 918, 1122, 1948, 1972, 2012
Hpy188III TCNNGA 6 cut(s) 65, 353, 815, 1558, 1988, 1995
Hpy99I CGWCG 1 cut(s) 918
HpyAV CCTTC 9 cut(s) 68, 334, 493, 710, 821, 1176, 1358, 1498, 1934
HpyCH4III ACNGT 9 cut(s) 113, 727, 761, 835, 1115, 1237, 1383, 1604, 1719
HpyCH4IV ACGT 3 cut(s) 144, 823, 2006
HpyF10VI GCNNNNNNNGC 8 cut(s) 10, 29, 148, 500, 1178, 1207, 1532, 1574
HpyF3I CTNAG 4 cut(s) 83, 1121, 1471, 1969
HpySE526I ACGT 3 cut(s) 144, 823, 2006
Kzo9I GATC 5 cut(s) 583, 817, 1590, 1661, 1943
LguI GCTCTTC 3 cut(s) 179, 429, 1771
Lsp1109I GCAGC 6 cut(s) 41, 249, 650, 873, 1423, 1589
LweI GCATC 4 cut(s) 397, 1198, 1322, 1488
MaeI CTAG 4 cut(s) 392, 845, 1295, 1695
MaeII ACGT 3 cut(s) 144, 823, 2006
MalI GATC 5 cut(s) 585, 819, 1592, 1663, 1945
MboI GATC 5 cut(s) 583, 817, 1590, 1661, 1943
MfeI CAATTG 2 cut(s) 24, 1389
MflI RGATCY 1 cut(s) 1661
MhlI GDGCHC 4 cut(s) 144, 424, 1783, 1987
MlyI GAGTC 1 cut(s) 1132
MmeI TCCRAC 1 cut(s) 896
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 2 cut(s) 78, 368
MseI TTAA 4 cut(s) 303, 789, 1056, 1955
MslI CAYNNNNRTG 3 cut(s) 1068, 1541, 1586
MspA1I CMGCKG 2 cut(s) 1331, 1436
MspI CCGG 4 cut(s) 179, 711, 1347, 1492
MspR9I CCNGG 7 cut(s) 179, 282, 682, 1279, 1347, 1348, 1361
MunI CAATTG 2 cut(s) 24, 1389
Mva1269I GAATGC 1 cut(s) 481
MvaI CCWGG 4 cut(s) 282, 682, 1279, 1361
MwoI GCNNNNNNNGC 8 cut(s) 10, 29, 148, 500, 1178, 1207, 1532, 1574
NciI CCSGG 3 cut(s) 179, 1347, 1348
NcoI CCATGG 1 cut(s) 383
NdeII GATC 5 cut(s) 583, 817, 1590, 1661, 1943
NlaIV GGNNCC 2 cut(s) 920, 1622
NmuCI GTSAC 4 cut(s) 548, 850, 1551, 1684
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 3 cut(s) 227, 986, 997
NspV TTCGAA 1 cut(s) 699
OliI CACNNNNGTG 2 cut(s) 1068, 1586
PagI TCATGA 1 cut(s) 1557
PciI ACATGT 1 cut(s) 993
PciSI GCTCTTC 3 cut(s) 179, 429, 1771
PctI GAATGC 1 cut(s) 481
PdmI GAANNNNTTC 2 cut(s) 78, 368
PfeI GAWTC 2 cut(s) 349, 1991
PfoI TCCNGGA 1 cut(s) 177
PkrI GCNGC 7 cut(s) 31, 229, 264, 640, 863, 1438, 1579
PleI GAGTC 1 cut(s) 1131
PpsI GAGTC 1 cut(s) 1131
PscI ACATGT 1 cut(s) 993
Psp6I CCWGG 4 cut(s) 280, 680, 1277, 1359
PspGI CCWGG 4 cut(s) 280, 680, 1277, 1359
PspN4I GGNNCC 2 cut(s) 920, 1622
PspPI GGNCC 2 cut(s) 1264, 1620
PstNI CAGNNNCTG 1 cut(s) 1976
PsuI RGATCY 1 cut(s) 1661
PvuII CAGCTG 1 cut(s) 1436
RsaI GTAC 4 cut(s) 390, 881, 1234, 1358
RsaNI GTAC 4 cut(s) 389, 880, 1233, 1357
RseI CAYNNNNRTG 3 cut(s) 1068, 1541, 1586
SapI GCTCTTC 3 cut(s) 179, 429, 1771
SaqAI TTAA 4 cut(s) 303, 789, 1056, 1955
SatI GCNGC 7 cut(s) 30, 228, 263, 639, 862, 1437, 1578
Sau3AI GATC 5 cut(s) 583, 817, 1590, 1661, 1943
Sau96I GGNCC 2 cut(s) 1264, 1620
SchI GAGTC 1 cut(s) 1132
ScrFI CCNGG 7 cut(s) 179, 282, 682, 1279, 1347, 1348, 1361
SduI GDGCHC 4 cut(s) 144, 424, 1783, 1987
SfaNI GCATC 4 cut(s) 397, 1198, 1322, 1488
SfcI CTRYAG 1 cut(s) 961
SfuI TTCGAA 1 cut(s) 699
SinI GGWCC 1 cut(s) 1620
SmaI CCCGGG 1 cut(s) 1348
SmiMI CAYNNNNRTG 3 cut(s) 1068, 1541, 1586
SmlI CTYRAG 3 cut(s) 665, 1625, 1986
SmoI CTYRAG 3 cut(s) 665, 1625, 1986
SpeI ACTAGT 1 cut(s) 1294
SsiI CCGC 6 cut(s) 228, 410, 452, 1061, 1329, 1835
SspMI CTAG 4 cut(s) 392, 845, 1295, 1695
StyD4I CCNGG 7 cut(s) 177, 280, 680, 1277, 1345, 1346, 1359
StyI CCWWGG 2 cut(s) 383, 485
TaaI ACNGT 9 cut(s) 113, 727, 761, 835, 1115, 1237, 1383, 1604, 1719
TaiI ACGT 3 cut(s) 147, 826, 2009
TaqI TCGA 2 cut(s) 249, 699
TatI WGTACW 2 cut(s) 879, 1232
TauI GCSGC 1 cut(s) 230
TfiI GAWTC 2 cut(s) 349, 1991
Tru1I TTAA 4 cut(s) 303, 789, 1056, 1955
Tru9I TTAA 4 cut(s) 303, 789, 1056, 1955
TseFI GTSAC 4 cut(s) 548, 850, 1551, 1684
TseI GCWGC 6 cut(s) 29, 262, 638, 861, 1436, 1577
Tsp45I GTSAC 4 cut(s) 548, 850, 1551, 1684
TspDTI ATGAA 7 cut(s) 40, 87, 319, 416, 659, 722, 1523
TspGWI ACGGA 3 cut(s) 424, 986, 1098
TspMI CCCGGG 1 cut(s) 1346
VpaK11BI GGWCC 1 cut(s) 1620
XapI RAATTY 1 cut(s) 1052
XceI RCATGY 3 cut(s) 227, 986, 997
XmaI CCCGGG 1 cut(s) 1346
XmiI GTMKAC 3 cut(s) 1858, 1870, 1882
XmnI GAANNNNTTC 2 cut(s) 78, 368
XspI CTAG 4 cut(s) 392, 845, 1295, 1695
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.