Rw2G033660

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001058
Physical Location & Seq
Forward (+)
55041488 .. 55043759
2272 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G033660.1

Sequence Viewer

Length: 2022 bp
ATGATTCCAGGGCTATTTGAAGCAATTGCAAGAAATGACACGCGGACTTTCATCGACTTGGTTGAAAACAATGGAGGTTCTCTCATTCAGAAAACTGAGGAAACAAAAAACTCGGTGTTACACCTTGCTGCAAGGTTTGGCCACGTTGAGCTGGTTGCCTATATCACTAAGCGGCTTCCTGACTTGGTTTCCGTCGGAAACATTAAGGGAGAAACTCCATTACATGAAGCTTGTCGCCAAGGGAATACCAGTGTTGTAAAGCTGCTGTTAGAGGTCAATCCATATGTGGCTTGTAGTCTGAACCACGAAATGCAAACTGCTTTCTACATGGCTTGTAGTCATGGAAATGTTGATGTTGTGAAGCACATGACAAACCAACCAGGGGTGTTAGACGCTGAAGAACATGAAGTCTGCAGCCCTCTACATCTAGCTATAGCAGAAGGCTTTATCGATATTGTTAAGAGTATTTTAGAAGCTTGCCCCAAGTTTGCTTGGAAGGTTGACAGCAAAGGACTTTTGCCGCTCCATTTGGCTTGCGAAACAGGTCACGTAGAGATAACAAGAACACTCTTGACGATTGATCCAAACCTTGCTCTTGAGTTTAACCCTTACGGTTACACACCACTACACTTGGCTGCGATGAATGGTCAAATCCAAATCCTTGAGGAACTTTTACTTTGCTCTCCCAAATCTTTAGAACTTCTCACACGAGACAGGGAAACTGTCTTTCATCTTTCAGTAAGATTCAACAGATACAGTATGTTTACCTCCTTGGCAAAGAATTATAATGACACTAACCTCCTCAACCAGGCTGACAAGCATGGTAACACAATCTTACATCTCACCGTCGCTGCAGGGAACACTAGATTATCGGAGTACATCATCAGAAATACTACGGTAGATGTCAATTTTAAGAACCAAAGAGGTGAAACAGCGTTGGACATCCTAAATCAGCAAAGAACATTATCTGATCTTAGAAACATGTTGAAGAGTTTTGGTGGCAGGACAAGTACTGAGCTGTTCCCTTTAACCCCAAAGGTTGATGAGATAGATTCTGAGACTACAACATTAGAAAGTCCTAGAGCTGCACTTGAGCAAGAATTTGAAATGCTTTTTGAAGGTAATATGCAACCAGGAAGTGATCAATATGATTGTGAAATTGGCAGGGGGCGCATGGCTAGAAACTGTACTGGTGTTATCCCATTCATGATGGATTCAATCCTCAGAAATAATCAACCACTGGAAGAATTCACTACAGTACAAGCGAAGATGAATCGATGTCATCAAGTTGTGATGAACTCTGATAATGATTTTGGCTCAAATTCTTCTAGAACAAGAATGAGCTCCCAAGTCTCGAATCCAAAGCAGATCTATAGGGAGGCATTGCAAAATACTAGGAACACTATCACAATAGTTGCTGTTTTGATTGCCTCGGTTACTTTTACTGCTGGTGTAAACCCTCCTGGTGGTGTCTACCAGGATGGACGGCTTAAAGGACAGTCAACTGTTGGCAGAACAACAGCATTTAAGGTCTTTACAATAAGCAACACAGCAGCATTGTTCATATCCATGTGCATTGTCATTGTGTTGGTGAGCATCATTCCCTTCCGGCGAAAATCACTGATGAAACTACTTGTGATTGCTCACAAAGTCTTGTGGGTGGCGGTTTCCCTCATGGCTACAGCTTATATTGCAGCGTCATGGATAATCATGCCACATGGACACGGTGAAGGAGAAAACTGGACCCTTGAAGTTGTGATATCTGTGTGTGCAGGGACTATGGGATTTGTTTTTCTTTGTCTTGGTGTCACATGGACTAAACACAGGTTGATGAAACTGAAATGGAGGAAACAGATATGTAAGACTGCAGAGGAAAGAGCACATACACAAGCAGCACTAGCACAAGAACAAGCACATAAAAGGGCGCAGCAAATAACACAGGATACTCCAGAATACAGCTTTTCAGTATCCATGAACGACCATGCTGAGGGTTTCGAAGAACTAGGATGCCATACTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

673

Amino Acids

75.09

Weight (kDa)

6.46

Isoelectric Point (pI)

28.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 6 - 92 2.3e-10 Ankyrin repeats (3 copies)
Ank_4 PF13637 40 - 90 2.2e-08 Ankyrin repeats (many copies)
Ank PF00023 70 - 93 8.2e-06 Ankyrin repeat
Ank_2 PF12796 102 - 158 1e-05 Ankyrin repeats (3 copies)
Ank_2 PF12796 141 - 225 5.5e-15 Ankyrin repeats (3 copies)
PGG PF13962 460 - 569 4.8e-31 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 786
AccBSI CCGCTC 1 cut(s) 523
AccI GTMKAC 1 cut(s) 1473
AccII CGCG 1 cut(s) 43
AciI CCGC 4 cut(s) 43, 172, 521, 1664
AclWI GGATC 1 cut(s) 575
AcoI YGGCCR 1 cut(s) 139
AcsI RAATTY 3 cut(s) 1100, 1247, 1321
AcuI CTGAAG 1 cut(s) 417
AfaI GTAC 4 cut(s) 878, 1012, 1189, 1260
AfiI CCNNNNNNNGG 4 cut(s) 382, 808, 1466, 1987
AflIII ACRYGT 1 cut(s) 981
AgsI TTSAA 8 cut(s) 20, 65, 748, 988, 1106, 1118, 1218, 1751
AjnI CCWGG 6 cut(s) 7, 379, 807, 1132, 1462, 1476
Alw21I GWGCWC 2 cut(s) 1346, 1882
Alw26I GTCTC 3 cut(s) 705, 1052, 1357
AlwI GGATC 1 cut(s) 575
AoxI GGCC 1 cut(s) 139
ApoI RAATTY 3 cut(s) 1100, 1247, 1321
AspLEI GCGC 2 cut(s) 1173, 1927
AspS9I GGNCC 1 cut(s) 1743
AsuHPI GGTGA 4 cut(s) 835, 938, 1603, 1739
AsuII TTCGAA 1 cut(s) 1995
AvaII GGWCC 1 cut(s) 1743
BalI TGGCCA 1 cut(s) 141
BanII GRGCYC 1 cut(s) 1346
BauI CACGAG 1 cut(s) 708
Bbv12I GWGCWC 2 cut(s) 1346, 1882
BbvCI CCTCAGC 1 cut(s) 1986
BccI CCATC 2 cut(s) 1204, 1475
BceAI ACGGC 1 cut(s) 1502
BciT130I CCWGG 6 cut(s) 9, 381, 809, 1134, 1464, 1478
BciVI GTATCC 2 cut(s) 1936, 1978
BclI TGATCA 1 cut(s) 1141
BcoDI GTCTC 3 cut(s) 705, 1052, 1357
BfaI CTAG 8 cut(s) 428, 864, 1080, 1179, 1329, 1395, 1898, 2003
BfmI CTRYAG 7 cut(s) 412, 432, 852, 1254, 1372, 1680, 1866
BfuI GTATCC 2 cut(s) 1936, 1978
BglII AGATCT 1 cut(s) 1368
BmcAI AGTACT 1 cut(s) 1012
Bme1390I CCNGG 6 cut(s) 9, 381, 809, 1134, 1464, 1478
Bme18I GGWCC 1 cut(s) 1743
BmgT120I GGNCC 1 cut(s) 1743
BmiI GGNNCC 1 cut(s) 1745
BmrFI CCNGG 6 cut(s) 9, 381, 809, 1134, 1464, 1478
BmsI GCATC 2 cut(s) 1605, 1997
BplI GAGNNNNNCTC 2 cut(s) 66, 98
BpmI CTGGAG 1 cut(s) 1932
Bpu10I CCTNAGC 1 cut(s) 1986
Bpu14I TTCGAA 1 cut(s) 1995
BpuEI CTTGAG 3 cut(s) 617, 683, 1112
Bsa29I ATCGAT 2 cut(s) 450, 1276
BsaAI YACGTR 1 cut(s) 550
BsaJI CCNNGG 5 cut(s) 8, 238, 380, 771, 1431
Bsc4I CCNNNNNNNGG 4 cut(s) 382, 808, 1466, 1987
Bse1I ACTGG 4 cut(s) 249, 1195, 1245, 1745
Bse3DI GCAATG 1 cut(s) 1382
BseBI CCWGG 6 cut(s) 9, 381, 809, 1134, 1464, 1478
BseCI ATCGAT 2 cut(s) 450, 1276
BseDI CCNNGG 5 cut(s) 8, 238, 380, 771, 1431
BseGI GGATG 3 cut(s) 942, 1486, 2012
BseLI CCNNNNNNNGG 4 cut(s) 382, 808, 1466, 1987
BseMI GCAATG 1 cut(s) 1382
BseMII CTCAG 5 cut(s) 87, 1005, 1047, 1237, 1977
BseNI ACTGG 4 cut(s) 249, 1195, 1245, 1745
BseRI GAGGAG 1 cut(s) 791
BsgI GTGCAG 2 cut(s) 1071, 1791
Bsh1236I CGCG 1 cut(s) 43
BshFI GGCC 1 cut(s) 141
BshVI ATCGAT 2 cut(s) 450, 1276
BsiHKAI GWGCWC 2 cut(s) 1346, 1882
BsiSI CCGG 1 cut(s) 1609
BslFI GGGAC 1 cut(s) 1789
BslI CCNNNNNNNGG 4 cut(s) 382, 808, 1466, 1987
BsmAI GTCTC 3 cut(s) 705, 1052, 1357
BsmFI GGGAC 1 cut(s) 1789
BsnI GGCC 1 cut(s) 141
Bsp119I TTCGAA 1 cut(s) 1995
Bsp1286I GDGCHC 2 cut(s) 1346, 1882
Bsp143I GATC 4 cut(s) 580, 970, 1141, 1368
BspACI CCGC 4 cut(s) 43, 172, 521, 1664
BspANI GGCC 1 cut(s) 141
BspCNI CTCAG 5 cut(s) 88, 1006, 1048, 1236, 1978
BspDI ATCGAT 2 cut(s) 450, 1276
BspFNI CGCG 1 cut(s) 43
BspHI TCATGA 1 cut(s) 1206
BspLI GGNNCC 1 cut(s) 1745
BspMAI CTGCAG 3 cut(s) 416, 856, 1870
BspPI GGATC 1 cut(s) 575
BspT104I TTCGAA 1 cut(s) 1995
BsrBI CCGCTC 1 cut(s) 523
BsrDI GCAATG 1 cut(s) 1382
BsrI ACTGG 4 cut(s) 249, 1195, 1245, 1745
BssECI CCNNGG 5 cut(s) 8, 238, 380, 771, 1431
BssMI GATC 4 cut(s) 580, 970, 1141, 1368
BssSI CACGAG 1 cut(s) 708
BssT1I CCWWGG 2 cut(s) 238, 771
Bst2BI CACGAG 1 cut(s) 708
Bst2UI CCWGG 6 cut(s) 9, 381, 809, 1134, 1464, 1478
Bst6I CTCTTC 1 cut(s) 983
BstBAI YACGTR 1 cut(s) 550
BstBI TTCGAA 1 cut(s) 1995
BstC8I GCNNGC 2 cut(s) 478, 535
BstDEI CTNAG 7 cut(s) 96, 168, 974, 1014, 1056, 1223, 1986
BstENI CCTNNNNNAGG 1 cut(s) 806
BstF5I GGATG 3 cut(s) 942, 1486, 2012
BstFNI CGCG 1 cut(s) 43
BstHHI GCGC 2 cut(s) 1173, 1927
BstKTI GATC 4 cut(s) 583, 973, 1144, 1371
BstMAI GTCTC 3 cut(s) 705, 1052, 1357
BstMBI GATC 4 cut(s) 580, 970, 1141, 1368
BstMWI GCNNNNNNNGC 3 cut(s) 1170, 1691, 1898
BstNI CCWGG 6 cut(s) 9, 381, 809, 1134, 1464, 1478
BstNSI RCATGY 1 cut(s) 985
BstSCI CCNGG 6 cut(s) 7, 379, 807, 1132, 1462, 1476
BstSFI CTRYAG 7 cut(s) 412, 432, 852, 1254, 1372, 1680, 1866
BstUI CGCG 1 cut(s) 43
BstX2I RGATCY 1 cut(s) 1368
BstYI RGATCY 1 cut(s) 1368
Bsu15I ATCGAT 2 cut(s) 450, 1276
BsuI GTATCC 2 cut(s) 1936, 1978
BsuRI GGCC 1 cut(s) 141
BsuTUI ATCGAT 2 cut(s) 450, 1276
BtgZI GCGATG 1 cut(s) 653
BtsCI GGATG 3 cut(s) 942, 1486, 2012
BtsIMutI CAGTG 3 cut(s) 256, 1238, 1619
Cac8I GCNNGC 2 cut(s) 478, 535
CciI TCATGA 1 cut(s) 1206
CfoI GCGC 2 cut(s) 1173, 1927
Cfr13I GGNCC 1 cut(s) 1743
ClaI ATCGAT 2 cut(s) 450, 1276
CseI GACGC 2 cut(s) 401, 1686
Csp6I GTAC 4 cut(s) 877, 1011, 1188, 1259
CspCI CAANNNNNGTGG 2 cut(s) 612, 647
CviQI GTAC 4 cut(s) 877, 1011, 1188, 1259
DdeI CTNAG 7 cut(s) 96, 168, 974, 1014, 1056, 1223, 1986
DpnI GATC 4 cut(s) 582, 972, 1143, 1370
DpnII GATC 4 cut(s) 580, 970, 1141, 1368
EaeI YGGCCR 1 cut(s) 139
Eam1104I CTCTTC 1 cut(s) 983
EarI CTCTTC 1 cut(s) 983
Ecl136II GAGCTC 1 cut(s) 1344
Eco130I CCWWGG 2 cut(s) 238, 771
Eco24I GRGCYC 1 cut(s) 1346
Eco32I GATATC 1 cut(s) 1761
Eco47I GGWCC 1 cut(s) 1743
Eco53kI GAGCTC 1 cut(s) 1344
Eco57I CTGAAG 1 cut(s) 417
EcoICRI GAGCTC 1 cut(s) 1344
EcoNI CCTNNNNNAGG 1 cut(s) 806
EcoRI GAATTC 1 cut(s) 1247
EcoRII CCWGG 6 cut(s) 7, 379, 807, 1132, 1462, 1476
EcoRV GATATC 1 cut(s) 1761
EcoT14I CCWWGG 2 cut(s) 238, 771
EcoT38I GRGCYC 1 cut(s) 1346
ErhI CCWWGG 2 cut(s) 238, 771
FaqI GGGAC 1 cut(s) 1789
FauNDI CATATG 1 cut(s) 283
FbaI TGATCA 1 cut(s) 1141
FblI GTMKAC 1 cut(s) 1473
FokI GGATG 2 cut(s) 929, 1493
FriOI GRGCYC 1 cut(s) 1346
FspBI CTAG 8 cut(s) 428, 864, 1080, 1179, 1329, 1395, 1898, 2003
GlaI GCGC 2 cut(s) 1172, 1926
GsuI CTGGAG 1 cut(s) 1932
HaeIII GGCC 1 cut(s) 141
HapII CCGG 1 cut(s) 1609
HgaI GACGC 2 cut(s) 401, 1686
HhaI GCGC 2 cut(s) 1173, 1927
Hin6I GCGC 2 cut(s) 1171, 1925
HinP1I GCGC 2 cut(s) 1171, 1925
HincII GTYRAC 2 cut(s) 502, 1503
HindII GTYRAC 2 cut(s) 502, 1503
HindIII AAGCTT 2 cut(s) 228, 474
HinfI GANTC 6 cut(s) 4, 744, 1052, 1214, 1273, 1357
HpaII CCGG 1 cut(s) 1609
HphI GGTGA 4 cut(s) 835, 938, 1603, 1739
Hpy166II GTNNAC 5 cut(s) 502, 765, 1456, 1474, 1503
Hpy188I TCNGA 9 cut(s) 90, 197, 300, 874, 887, 970, 1057, 1226, 1303
Hpy188III TCNNGA 7 cut(s) 179, 571, 596, 1207, 1329, 1354, 1949
Hpy8I GTNNAC 5 cut(s) 502, 765, 1456, 1474, 1503
Hpy99I CGWCG 2 cut(s) 197, 851
HpyAV CCTTC 5 cut(s) 434, 490, 1112, 1615, 1724
HpyCH4IV ACGT 2 cut(s) 144, 549
HpyF10VI GCNNNNNNNGC 3 cut(s) 1170, 1691, 1898
HpyF3I CTNAG 7 cut(s) 96, 168, 974, 1014, 1056, 1223, 1986
HpySE526I ACGT 2 cut(s) 144, 549
HspAI GCGC 2 cut(s) 1171, 1925
Ksp22I TGATCA 1 cut(s) 1141
Kzo9I GATC 4 cut(s) 580, 970, 1141, 1368
LmnI GCTCC 2 cut(s) 528, 1349
LweI GCATC 2 cut(s) 1605, 1997
MaeI CTAG 8 cut(s) 428, 864, 1080, 1179, 1329, 1395, 1898, 2003
MaeII ACGT 2 cut(s) 144, 549
MaeIII GTNAC 6 cut(s) 117, 545, 614, 824, 1435, 1807
MalI GATC 4 cut(s) 582, 972, 1143, 1370
MbiI CCGCTC 1 cut(s) 523
MboI GATC 4 cut(s) 580, 970, 1141, 1368
MboII GAAGA 6 cut(s) 410, 1000, 1256, 1279, 1317, 2009
MfeI CAATTG 1 cut(s) 24
MflI RGATCY 1 cut(s) 1368
MhlI GDGCHC 2 cut(s) 1346, 1882
MlsI TGGCCA 1 cut(s) 141
MluCI AATT 7 cut(s) 24, 781, 907, 1100, 1158, 1247, 1321
MluNI TGGCCA 1 cut(s) 141
MmeI TCCRAC 2 cut(s) 175, 918
Mox20I TGGCCA 1 cut(s) 141
MscI TGGCCA 1 cut(s) 141
MseI TTAA 7 cut(s) 204, 459, 603, 912, 1028, 1491, 1527
MslI CAYNNNNRTG 2 cut(s) 345, 1568
Msp20I TGGCCA 1 cut(s) 141
MspI CCGG 1 cut(s) 1609
MspR9I CCNGG 6 cut(s) 9, 381, 809, 1134, 1464, 1478
MunI CAATTG 1 cut(s) 24
MvaI CCWGG 6 cut(s) 9, 381, 809, 1134, 1464, 1478
MvnI CGCG 1 cut(s) 43
MwoI GCNNNNNNNGC 3 cut(s) 1170, 1691, 1898
NdeI CATATG 1 cut(s) 283
NdeII GATC 4 cut(s) 580, 970, 1141, 1368
NlaIV GGNNCC 1 cut(s) 1745
NmuCI GTSAC 2 cut(s) 545, 1807
NspI RCATGY 1 cut(s) 985
NspV TTCGAA 1 cut(s) 1995
PagI TCATGA 1 cut(s) 1206
PciI ACATGT 1 cut(s) 981
PfeI GAWTC 6 cut(s) 4, 744, 1052, 1214, 1273, 1357
Ppu21I YACGTR 1 cut(s) 550
PscI ACATGT 1 cut(s) 981
PsiI TTATAA 1 cut(s) 786
Psp124BI GAGCTC 1 cut(s) 1346
Psp6I CCWGG 6 cut(s) 7, 379, 807, 1132, 1462, 1476
PspGI CCWGG 6 cut(s) 7, 379, 807, 1132, 1462, 1476
PspN4I GGNNCC 1 cut(s) 1745
PspPI GGNCC 1 cut(s) 1743
PstI CTGCAG 3 cut(s) 416, 856, 1870
PsuI RGATCY 1 cut(s) 1368
RsaI GTAC 4 cut(s) 878, 1012, 1189, 1260
RsaNI GTAC 4 cut(s) 877, 1011, 1188, 1259
RseI CAYNNNNRTG 2 cut(s) 345, 1568
SacI GAGCTC 1 cut(s) 1346
SaqAI TTAA 7 cut(s) 204, 459, 603, 912, 1028, 1491, 1527
Sau3AI GATC 4 cut(s) 580, 970, 1141, 1368
Sau96I GGNCC 1 cut(s) 1743
ScaI AGTACT 1 cut(s) 1012
ScrFI CCNGG 6 cut(s) 9, 381, 809, 1134, 1464, 1478
SduI GDGCHC 2 cut(s) 1346, 1882
SfaNI GCATC 2 cut(s) 1605, 1997
SfcI CTRYAG 7 cut(s) 412, 432, 852, 1254, 1372, 1680, 1866
SfuI TTCGAA 1 cut(s) 1995
SinI GGWCC 1 cut(s) 1743
SmiMI CAYNNNNRTG 2 cut(s) 345, 1568
SmlI CTYRAG 3 cut(s) 596, 662, 1091
SmoI CTYRAG 3 cut(s) 596, 662, 1091
Sse9I AATT 7 cut(s) 24, 781, 907, 1100, 1158, 1247, 1321
SsiI CCGC 4 cut(s) 43, 172, 521, 1664
SspMI CTAG 8 cut(s) 428, 864, 1080, 1179, 1329, 1395, 1898, 2003
SstI GAGCTC 1 cut(s) 1346
StyD4I CCNGG 6 cut(s) 7, 379, 807, 1132, 1462, 1476
StyI CCWWGG 2 cut(s) 238, 771
TaiI ACGT 2 cut(s) 147, 552
TaqI TCGA 5 cut(s) 54, 450, 1276, 1355, 1995
TasI AATT 7 cut(s) 24, 781, 907, 1100, 1158, 1247, 1321
TatI WGTACW 4 cut(s) 876, 1010, 1187, 1258
TauI GCSGC 2 cut(s) 175, 523
TfiI GAWTC 6 cut(s) 4, 744, 1052, 1214, 1273, 1357
Tru1I TTAA 7 cut(s) 204, 459, 603, 912, 1028, 1491, 1527
Tru9I TTAA 7 cut(s) 204, 459, 603, 912, 1028, 1491, 1527
TscAI CASTG 3 cut(s) 256, 1245, 1626
TseFI GTSAC 2 cut(s) 545, 1807
Tsp45I GTSAC 2 cut(s) 545, 1807
TspGWI ACGGA 1 cut(s) 181
TspRI CASTG 3 cut(s) 256, 1245, 1626
VpaK11BI GGWCC 1 cut(s) 1743
XagI CCTNNNNNAGG 1 cut(s) 806
XapI RAATTY 3 cut(s) 1100, 1247, 1321
XbaI TCTAGA 1 cut(s) 1328
XceI RCATGY 1 cut(s) 985
XmiI GTMKAC 1 cut(s) 1473
XspI CTAG 8 cut(s) 428, 864, 1080, 1179, 1329, 1395, 1898, 2003
ZrmI AGTACT 1 cut(s) 1012
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.