Rmu_sc0000545.1_g000024

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000545.1
OG0001058
Physical Location & Seq
Forward (+)
111576 .. 114187
2612 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000545.1_g000024.1.cds

Sequence Viewer

Length: 2310 bp
atgattccagcagggctatatgaagcaattgcaagaaatgacactcagactttcaaggacattgttcgatacaatggaggatctctcttgcagaaaactgaagaaacaagagactcagtgttacaccttgctgccaggttcgggcatgttgagctggttgcctatatcatcactctgcatcctgactttgtttctgttaggaacgttaagggagaaactccgttacacaaagcttgccgccactggaataccaatgttgtgaagctgctcttagaggccaatccatatgtggttggtgctcttaaccatgaaatgcaaactgctttttacatagcctgcagttgtgggaatgttgatgttgtcaagtacatggtaaaccaaccaggggtgttagactttgaacaacatgaagcttccagtcctcttcatcattctatagcagaaggctatgatgtcgatattgtcaagactattttggaagcatgtccccagtttgcttggaagtttgacaacaatgggtatttgccgctgcatttggcttgcgagaaaggtcaggtagagatagtaaaaacactcttgaggttcaatccaaacctttctcttgaacacaatcgctaccgttacacaccactgcacgtggctgcaatgaatggtcaaatccaaatccttgaggaacttcttttgtggtctcccggatccttgaaagtttgcacaggaaacggagaaaatgtgtttcatctttctgtaagattcaacagatatgatgtgttcacctctttggcaaagaattataaccgcaccagcctgctcaaccaggctgacaagcagggtaacacagtcttacatcttgctgtctctgcagggaacactagattatcagagtacatcatcagcaatactgcagtagatgtcaattgtaggaaccaaagaggtcaaacagcattggacatcctaaacgagcaaagcagttcggctgatcttagacatacgttgaagagatttggtggcagaacaagtattgagctgttctctttaaccccaaaggttgatgagatatattctgggactacaacaacatcagaatgtcctagagctgcactggagaaagaatttgatagggttttatgtaacacgcgtccaggaagtgatcaatctgattgtgaaattggcatgggccgtatggctcgaaacaatcaacaaatggaaaactttgctacagtacaagcaaagatgaatcccagtcatgaagttgtgataaattctgatattccttttagctcagaatcgaatagaacaagtatgagctccccagtcttcagtccacagaagcttcgaaagccaggagggaaagtgggtaaaagtaagcaatatgagatatatagagaggcattgcaaaatactaggaacacgatcacaatagttgctgttttgattgcctcagttacttttactgctggcataaacccacctggaggtgtctaccaggagggatggcttaaaggacagtcagtagttggcagaacaacagcttttaaggtcttcacaataagtaacacagcggcattgttcatatctatgtgcattgttattttattggtgagcatcattcccttccggcgaaaatcactgatgaaactacttgtggttgctcacaaagtcttgtgggtggcggtgtcactcatggccacagcttacattgcagcctcatggataatcttgccacatggacgtggaaacagcaacgtacaactgcaacacgagcacagaaacaagcacaaaggcaagcagcacaagcagagcaacatgcacaaagacaagcagcacgagcagagcaacaagcacaaaaacaagcagcacgagcacagcaagaagcacaaagacaagcagcacgagcacagaaacaagcacaaagacaagcagcacgagcacagcaacaagcacaaagacaagcagcacgagcagagcaacaagcacaaaaacaagccgagcaacatgcacaaagacaagcagcacgagcacagaaacaagcacaaagacaagcagcacgagcagagcaacaagcacaaagacaagcagcacgagcagagcaacaagcacaaaaacaagcagagcaacatgcacgaagacaagcagcacgagcagagcaacaagcacaaaaacaagcagcacgagcacagcaagaagcacaaagacaagcagcacgagcacagaaacgagagcataaaatggcacagagaagaacagaggaaacacaagaatatagctcgtcagtatccacgaactcccctgctgagagttgcaattcactag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

769

Amino Acids

88.07

Weight (kDa)

9.61

Isoelectric Point (pI)

49.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 792
AccI GTMKAC 1 cut(s) 1479
AccII CGCG 1 cut(s) 1135
AciI CCGC 5 cut(s) 238, 527, 796, 1559, 1670
AclI AACGTT 1 cut(s) 204
AclWI GGATC 3 cut(s) 88, 690, 703
AcoI YGGCCR 1 cut(s) 1683
AcsI RAATTY 2 cut(s) 1109, 1258
AcuI CTGAAG 2 cut(s) 120, 1300
AcvI CACGTG 1 cut(s) 637
AfaI GTAC 4 cut(s) 368, 884, 1221, 1746
AfiI CCNNNNNNNGG 3 cut(s) 141, 385, 1472
AflIII ACRYGT 1 cut(s) 1133
AgsI TTSAA 7 cut(s) 55, 401, 586, 605, 703, 754, 994
AjiI CACGTC 1 cut(s) 1730
AjnI CCWGG 7 cut(s) 134, 382, 813, 1138, 1339, 1468, 1482
Alw21I GWGCWC 9 cut(s) 301, 1307, 1764, 1863, 1896, 1929, 2019, 2175, 2208
Alw26I GTCTC 3 cut(s) 105, 693, 859
AlwI GGATC 3 cut(s) 88, 690, 703
AoxI GGCC 3 cut(s) 276, 1174, 1683
ApoI RAATTY 2 cut(s) 1109, 1258
AspS9I GGNCC 1 cut(s) 1174
AsuC2I CCSGG 1 cut(s) 693
AsuHPI GGTGA 2 cut(s) 763, 1609
AsuII TTCGAA 1 cut(s) 1333
BalI TGGCCA 1 cut(s) 1685
BamHI GGATCC 1 cut(s) 695
BanII GRGCYC 1 cut(s) 1307
BbrPI CACGTG 1 cut(s) 637
BbsI GAAGAC 3 cut(s) 1306, 1531, 2130
Bbv12I GWGCWC 9 cut(s) 301, 1307, 1764, 1863, 1896, 1929, 2019, 2175, 2208
BccI CCATC 1 cut(s) 1485
BceAI ACGGC 1 cut(s) 1161
BcgI CGANNNNNNTGC 2 cut(s) 1975, 2009
BciT130I CCWGG 7 cut(s) 136, 384, 815, 1140, 1341, 1470, 1484
BciVI GTATCC 1 cut(s) 2283
BclI TGATCA 1 cut(s) 1147
BcnI CCSGG 1 cut(s) 693
BcoDI GTCTC 3 cut(s) 105, 693, 859
BfaI CTAG 4 cut(s) 870, 1089, 1401, 2308
BfmI CTRYAG 5 cut(s) 337, 435, 858, 900, 1215
BfuI GTATCC 1 cut(s) 2283
Bme1390I CCNGG 8 cut(s) 136, 384, 693, 815, 1140, 1341, 1470, 1484
BmgBI CACGTC 1 cut(s) 1730
BmgT120I GGNCC 1 cut(s) 1174
BmiI GGNNCC 2 cut(s) 697, 923
BmrFI CCNGG 8 cut(s) 136, 384, 693, 815, 1140, 1341, 1470, 1484
BmrI ACTGGG 3 cut(s) 484, 1233, 1304
BmsI GCATC 2 cut(s) 187, 1611
BmuI ACTGGG 3 cut(s) 484, 1233, 1304
BpiI GAAGAC 3 cut(s) 1306, 1531, 2130
BplI GAGNNNNNCTC 4 cut(s) 69, 101, 1013, 1045
BpmI CTGGAG 2 cut(s) 1121, 1491
Bpu14I TTCGAA 1 cut(s) 1333
BpuEI CTTGAG 2 cut(s) 598, 689
BpuMI CCSGG 1 cut(s) 693
BsaAI YACGTR 1 cut(s) 637
BsaI GGTCTC 1 cut(s) 693
BsaJI CCNNGG 1 cut(s) 383
BsaXI ACNNNNNCTCC 4 cut(s) 714, 744, 1335, 1365
Bsc4I CCNNNNNNNGG 3 cut(s) 141, 385, 1472
Bse1I ACTGG 6 cut(s) 248, 417, 490, 1104, 1239, 1310
Bse3DI GCAATG 3 cut(s) 651, 1388, 1695
BseBI CCWGG 7 cut(s) 136, 384, 815, 1140, 1341, 1470, 1484
BseDI CCNNGG 1 cut(s) 383
BseGI GGATG 3 cut(s) 178, 948, 1496
BseLI CCNNNNNNNGG 3 cut(s) 141, 385, 1472
BseMI GCAATG 3 cut(s) 651, 1388, 1695
BseMII CTCAG 5 cut(s) 59, 129, 1293, 1452, 2282
BseNI ACTGG 6 cut(s) 248, 417, 490, 1104, 1239, 1310
BsgI GTGCAG 2 cut(s) 617, 1080
Bsh1236I CGCG 1 cut(s) 1135
BshFI GGCC 3 cut(s) 278, 1176, 1685
BsiHKAI GWGCWC 9 cut(s) 301, 1307, 1764, 1863, 1896, 1929, 2019, 2175, 2208
BsiSI CCGG 2 cut(s) 693, 1615
BslFI GGGAC 2 cut(s) 471, 1078
BslI CCNNNNNNNGG 3 cut(s) 141, 385, 1472
BsmAI GTCTC 3 cut(s) 105, 693, 859
BsmFI GGGAC 2 cut(s) 471, 1078
BsnI GGCC 3 cut(s) 278, 1176, 1685
Bso31I GGTCTC 1 cut(s) 693
Bsp119I TTCGAA 1 cut(s) 1333
Bsp1286I GDGCHC 9 cut(s) 301, 1307, 1764, 1863, 1896, 1929, 2019, 2175, 2208
Bsp143I GATC 5 cut(s) 80, 695, 976, 1147, 1410
BspACI CCGC 5 cut(s) 238, 527, 796, 1559, 1670
BspANI GGCC 3 cut(s) 278, 1176, 1685
BspCNI CTCAG 5 cut(s) 58, 128, 1292, 1451, 2283
BspFNI CGCG 1 cut(s) 1135
BspHI TCATGA 1 cut(s) 1243
BspLI GGNNCC 2 cut(s) 697, 923
BspMAI CTGCAG 3 cut(s) 341, 862, 904
BspPI GGATC 3 cut(s) 88, 690, 703
BspT104I TTCGAA 1 cut(s) 1333
BspTNI GGTCTC 1 cut(s) 693
BsrDI GCAATG 3 cut(s) 651, 1388, 1695
BsrI ACTGG 6 cut(s) 248, 417, 490, 1104, 1239, 1310
BssECI CCNNGG 1 cut(s) 383
BssMI GATC 5 cut(s) 80, 695, 976, 1147, 1410
Bst2UI CCWGG 7 cut(s) 136, 384, 815, 1140, 1341, 1470, 1484
Bst4CI ACNGT 4 cut(s) 620, 838, 1219, 1506
Bst6I CTCTTC 2 cut(s) 429, 989
BstBAI YACGTR 1 cut(s) 637
BstBI TTCGAA 1 cut(s) 1333
BstC8I GCNNGC 6 cut(s) 235, 337, 541, 806, 1456, 1784
BstDEI CTNAG 7 cut(s) 45, 115, 271, 980, 1279, 1438, 2291
BstF5I GGATG 3 cut(s) 178, 948, 1496
BstFNI CGCG 1 cut(s) 1135
BstKTI GATC 5 cut(s) 83, 698, 979, 1150, 1413
BstMAI GTCTC 3 cut(s) 105, 693, 859
BstMBI GATC 5 cut(s) 80, 695, 976, 1147, 1410
BstNI CCWGG 7 cut(s) 136, 384, 815, 1140, 1341, 1470, 1484
BstNSI RCATGY 5 cut(s) 149, 486, 1807, 1996, 2119
BstSCI CCNGG 8 cut(s) 134, 382, 691, 813, 1138, 1339, 1468, 1482
BstSFI CTRYAG 5 cut(s) 337, 435, 858, 900, 1215
BstUI CGCG 1 cut(s) 1135
BstV2I GAAGAC 3 cut(s) 1306, 1531, 2130
BstX2I RGATCY 2 cut(s) 80, 695
BstYI RGATCY 2 cut(s) 80, 695
BsuI GTATCC 1 cut(s) 2283
BsuRI GGCC 3 cut(s) 278, 1176, 1685
BtrI CACGTC 1 cut(s) 1730
BtsCI GGATG 3 cut(s) 178, 948, 1496
BtsI GCAGTG 1 cut(s) 629
BtsIMutI CAGTG 5 cut(s) 123, 241, 629, 1097, 1625
Cac8I GCNNGC 6 cut(s) 235, 337, 541, 806, 1456, 1784
CciI TCATGA 1 cut(s) 1243
Cfr13I GGNCC 1 cut(s) 1174
CseI GACGC 1 cut(s) 1124
Csp6I GTAC 4 cut(s) 367, 883, 1220, 1745
CviQI GTAC 4 cut(s) 367, 883, 1220, 1745
DdeI CTNAG 7 cut(s) 45, 115, 271, 980, 1279, 1438, 2291
DpnI GATC 5 cut(s) 82, 697, 978, 1149, 1412
DpnII GATC 5 cut(s) 80, 695, 976, 1147, 1410
EaeI YGGCCR 1 cut(s) 1683
Eam1104I CTCTTC 2 cut(s) 429, 989
EarI CTCTTC 2 cut(s) 429, 989
Ecl136II GAGCTC 1 cut(s) 1305
Eco24I GRGCYC 1 cut(s) 1307
Eco31I GGTCTC 1 cut(s) 693
Eco53kI GAGCTC 1 cut(s) 1305
Eco57I CTGAAG 2 cut(s) 120, 1300
Eco72I CACGTG 1 cut(s) 637
EcoICRI GAGCTC 1 cut(s) 1305
EcoRII CCWGG 7 cut(s) 134, 382, 813, 1138, 1339, 1468, 1482
EcoT38I GRGCYC 1 cut(s) 1307
FalI AAGNNNNNCTT 2 cut(s) 254, 286
FaqI GGGAC 2 cut(s) 471, 1078
FauNDI CATATG 1 cut(s) 286
FbaI TGATCA 1 cut(s) 1147
FblI GTMKAC 1 cut(s) 1479
FokI GGATG 3 cut(s) 165, 935, 1503
FriOI GRGCYC 1 cut(s) 1307
FspBI CTAG 4 cut(s) 870, 1089, 1401, 2308
GsuI CTGGAG 2 cut(s) 1121, 1491
HaeIII GGCC 3 cut(s) 278, 1176, 1685
HapII CCGG 2 cut(s) 693, 1615
HgaI GACGC 1 cut(s) 1124
HindIII AAGCTT 3 cut(s) 231, 411, 1328
HinfI GANTC 5 cut(s) 4, 113, 750, 1234, 1283
HpaII CCGG 2 cut(s) 693, 1615
HphI GGTGA 2 cut(s) 763, 1609
Hpy166II GTNNAC 4 cut(s) 376, 771, 1322, 1480
Hpy188I TCNGA 6 cut(s) 48, 880, 1081, 1156, 1264, 1282
Hpy188III TCNNGA 5 cut(s) 182, 466, 577, 602, 1244
Hpy8I GTNNAC 4 cut(s) 376, 771, 1322, 1480
HpyAV CCTTC 2 cut(s) 437, 1621
HpyCH4III ACNGT 4 cut(s) 620, 838, 1219, 1506
HpyCH4IV ACGT 5 cut(s) 204, 636, 989, 1729, 1743
HpyF3I CTNAG 7 cut(s) 45, 115, 271, 980, 1279, 1438, 2291
HpySE526I ACGT 5 cut(s) 204, 636, 989, 1729, 1743
Ksp22I TGATCA 1 cut(s) 1147
Kzo9I GATC 5 cut(s) 80, 695, 976, 1147, 1410
LmnI GCTCC 1 cut(s) 1310
LweI GCATC 2 cut(s) 187, 1611
MaeI CTAG 4 cut(s) 870, 1089, 1401, 2308
MaeII ACGT 5 cut(s) 204, 636, 989, 1729, 1743
MaeIII GTNAC 8 cut(s) 120, 222, 620, 830, 1127, 1441, 1550, 1674
MalI GATC 5 cut(s) 82, 697, 978, 1149, 1412
MboI GATC 5 cut(s) 80, 695, 976, 1147, 1410
MboII GAAGA 7 cut(s) 113, 416, 1006, 1306, 1531, 2135, 2249
MfeI CAATTG 2 cut(s) 27, 913
MflI RGATCY 2 cut(s) 80, 695
MhlI GDGCHC 9 cut(s) 301, 1307, 1764, 1863, 1896, 1929, 2019, 2175, 2208
MlsI TGGCCA 1 cut(s) 1685
MluCI AATT 7 cut(s) 27, 787, 913, 1109, 1164, 1258, 2301
MluI ACGCGT 1 cut(s) 1133
MluNI TGGCCA 1 cut(s) 1685
MlyI GAGTC 1 cut(s) 107
Mox20I TGGCCA 1 cut(s) 1685
MscI TGGCCA 1 cut(s) 1685
MseI TTAA 5 cut(s) 207, 303, 1034, 1497, 1533
MslI CAYNNNNRTG 3 cut(s) 1081, 1574, 1728
Msp20I TGGCCA 1 cut(s) 1685
MspA1I CMGCKG 2 cut(s) 529, 1559
MspI CCGG 2 cut(s) 693, 1615
MspR9I CCNGG 8 cut(s) 136, 384, 693, 815, 1140, 1341, 1470, 1484
MunI CAATTG 2 cut(s) 27, 913
MvaI CCWGG 7 cut(s) 136, 384, 815, 1140, 1341, 1470, 1484
MvnI CGCG 1 cut(s) 1135
NciI CCSGG 1 cut(s) 693
NdeI CATATG 1 cut(s) 286
NdeII GATC 5 cut(s) 80, 695, 976, 1147, 1410
NlaIV GGNNCC 2 cut(s) 697, 923
NmeAIII GCCGAG 1 cut(s) 2010
NmuCI GTSAC 1 cut(s) 1674
NspI RCATGY 5 cut(s) 149, 486, 1807, 1996, 2119
NspV TTCGAA 1 cut(s) 1333
PagI TCATGA 1 cut(s) 1243
PfeI GAWTC 4 cut(s) 4, 750, 1234, 1283
PfoI TCCNGGA 2 cut(s) 691, 1138
PleI GAGTC 1 cut(s) 107
PmaCI CACGTG 1 cut(s) 637
PmlI CACGTG 1 cut(s) 637
PpsI GAGTC 1 cut(s) 107
Ppu21I YACGTR 1 cut(s) 637
PsiI TTATAA 1 cut(s) 792
Psp124BI GAGCTC 1 cut(s) 1307
Psp1406I AACGTT 1 cut(s) 204
Psp6I CCWGG 7 cut(s) 134, 382, 813, 1138, 1339, 1468, 1482
PspCI CACGTG 1 cut(s) 637
PspGI CCWGG 7 cut(s) 134, 382, 813, 1138, 1339, 1468, 1482
PspN4I GGNNCC 2 cut(s) 697, 923
PspPI GGNCC 1 cut(s) 1174
PstI CTGCAG 3 cut(s) 341, 862, 904
PsuI RGATCY 2 cut(s) 80, 695
RsaI GTAC 4 cut(s) 368, 884, 1221, 1746
RsaNI GTAC 4 cut(s) 367, 883, 1220, 1745
RseI CAYNNNNRTG 3 cut(s) 1081, 1574, 1728
SacI GAGCTC 1 cut(s) 1307
SaqAI TTAA 5 cut(s) 207, 303, 1034, 1497, 1533
Sau3AI GATC 5 cut(s) 80, 695, 976, 1147, 1410
Sau96I GGNCC 1 cut(s) 1174
SchI GAGTC 1 cut(s) 107
ScrFI CCNGG 8 cut(s) 136, 384, 693, 815, 1140, 1341, 1470, 1484
SduI GDGCHC 9 cut(s) 301, 1307, 1764, 1863, 1896, 1929, 2019, 2175, 2208
SfaNI GCATC 2 cut(s) 187, 1611
SfcI CTRYAG 5 cut(s) 337, 435, 858, 900, 1215
SfuI TTCGAA 1 cut(s) 1333
SmiMI CAYNNNNRTG 3 cut(s) 1081, 1574, 1728
SmlI CTYRAG 2 cut(s) 577, 668
SmoI CTYRAG 2 cut(s) 577, 668
Sse9I AATT 7 cut(s) 27, 787, 913, 1109, 1164, 1258, 2301
SsiI CCGC 5 cut(s) 238, 527, 796, 1559, 1670
SspMI CTAG 4 cut(s) 870, 1089, 1401, 2308
SstI GAGCTC 1 cut(s) 1307
StyD4I CCNGG 8 cut(s) 134, 382, 691, 813, 1138, 1339, 1468, 1482
TaaI ACNGT 4 cut(s) 620, 838, 1219, 1506
TaiI ACGT 5 cut(s) 207, 639, 992, 1732, 1746
TaqI TCGA 5 cut(s) 67, 456, 1186, 1286, 1333
TasI AATT 7 cut(s) 27, 787, 913, 1109, 1164, 1258, 2301
TatI WGTACW 3 cut(s) 366, 882, 1219
TauI GCSGC 3 cut(s) 240, 529, 1562
TfiI GAWTC 4 cut(s) 4, 750, 1234, 1283
Tru1I TTAA 5 cut(s) 207, 303, 1034, 1497, 1533
Tru9I TTAA 5 cut(s) 207, 303, 1034, 1497, 1533
TscAI CASTG 5 cut(s) 123, 248, 636, 1104, 1632
TseFI GTSAC 1 cut(s) 1674
Tsp45I GTSAC 1 cut(s) 1674
TspGWI ACGGA 2 cut(s) 210, 735
TspRI CASTG 5 cut(s) 123, 248, 636, 1104, 1632
XapI RAATTY 2 cut(s) 1109, 1258
XceI RCATGY 5 cut(s) 149, 486, 1807, 1996, 2119
XcmI CCANNNNNNNNNTGG 1 cut(s) 286
XmiI GTMKAC 1 cut(s) 1479
XspI CTAG 4 cut(s) 870, 1089, 1401, 2308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.