RchiOBHm_Chr2g0140001

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0001058
Physical Location & Seq
Forward (+)
57367745 .. 57370077
2333 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ51048

Sequence Viewer

Length: 2073 bp
ATGATTCCAGGGCTATTTGAAGCAATTGCAAGAAATGACACGCGGACTTTCATCGACTTGGTTGAAAACAATGGAGGTTCTCTCATTCAGAAAACTGAGGAAACAAAAAACTCGGTGTTACACCTTGCTGCAAGGTTCGGCCACGTTGAGCTGGTTGCCTATATCACTAAGCGGCTTCCTGACTTGGTTTCCGTTGGAAACATTAAGGGAGAAACTCCATTACATGAAGCTTGTCGCCAAGGGAATACCAGTGTTGTAAAGCTGCTGTTAGAGGTCAATCCATATGTGGCTTGTAGTCTGAACCACGAAATGCAAACTGCTTTCTACATGGCTTGTAGTCATGGAAATGTTGATGTTGTGAAGCACATGACAAACCAACCAGGGGTGTTAGACGCTGAAGAACATGAAGTCTGCAGCCCTCTACATCTAGCTATAGCAGAAGGCTTTATCGATATTGTTAAGAGTATTTTAGAAGCTTGCCCCAAGTTTGCTTGGAAGGTTGACAGCAAAGGACTTTTGCCGCTCCATTTGGCTTGCGAAACAGGTCACGTAGAGATAACAAGAACACTCTTGACGATTGATCCAAACCTTGCTCTTGAGTTTAACCCTTACGGTTACACACCACTACACTTGGCTGCGATGAATGGTCAAATCCAAATCCTTGAGGAACTTTTACTTTGCTCTCCCAAATCTTTAGAACTTCTCACACGAGACAGGGAAACTGTCTTTCATCTTTCAGTAAGATTCAACAGATACAGTATGTTTACCTCCTTGGCAAAGAATTATAATGACACTAACCTCCTCAACCAGGCTGACAAGCATGGTAACACAATCTTACATCTCACCGTCGCTGCAGGGAACACTAGATTATCGGAGTACATCATCAGAAATACTACGGTAGATGTCAATTTTAAGAACCAAAGAGGTGAAACAGCATTGGACATCCTAAATCAGCAAAGAACATTATCTGATCTTAGAAACATGTTGAAGAGTTTTGGTGGCAGGACAAGTACTGAGCTGTTCCCTTTAACCCCAAAGGTTGATGAGATAGATTCTGAGACTACAACATTAGAAAGTCCTAGAGCTGCACTTGAGCAAGAATTTGAAATGCTTTTTGAAGGTAATATGCAACCAGGAAGTGATCAATATGATTGTGAAATTGGCAGGGGGCGCATGGCTAGAAACTGTACTGGTGTTATCCCATTCATGATGGATTCAATCCTCAGAAATAATCAACCACTGGAAGAATTCACTACAGTACAAGCGAAGATGAATCCATGTCATCAAGTTGTGATGAACTCTGATAATGATTTTGGCTCAAATTCTTCTAGAACAAGAATGAGCTCCCAAGTCTCGAATCCAAAGCAGGTTCCGAAGACAAGAGGAAAAAATAAAGTGGGTAAAACTAGGCAATATGAGATCTATAGGGAGGCATTGCAAAATACTAGGAACACTATCACAATAGTTGCTGTTTTGATTGCCTCGGTTACTTTTACTGCTGGCGTAAACCCTCCTGGTGGTGTCTACCAGGATGGACGGCTTAAAGGACAGTCAACTGTTGGCAGAACAACAGCATTTAAGGTCTTTACAATAAGCAACACAGCAGCATTGTTCATATCCATGTGCATTGTCATTGTGTTGGTGAGCATCATTCCCTTCCGGCGAAAATCACTGATGAAACTACTTGTGATTGCTCACAAAGTCTTGTGGGTGGCGGTTTCCCTCATGGCTACAGCTTATATTGCAGCGTCATGGATAATCATGCCACATGGACACGGTGAAGGAGAAAACTGGACCCTTGAACTTGTGATATCTGTGTGTGCAGGGACTATGGGATTTGTTTTTCTTTGTCTTGGTGTCACATGGACTAAACACAGGTTGATGAAACTGAAATGGAGGAAACAGATATGTAAGACTGCAGAGGAAAGAGCACATACACAAGCAGCACTAGCACAAGAACAAGCACATAAAAGGGCGCAGCAAATAACACAGGATACTCCAGAATACAGCTTTTCAGTATCCATGAACGACCATGCTGAGGGTTTCGAAGAACTAGGATGCCATACTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

690

Amino Acids

77.02

Weight (kDa)

6.91

Isoelectric Point (pI)

28.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 6 - 92 2.3e-10 Ankyrin repeats (3 copies)
Ank_4 PF13637 40 - 90 2.2e-08 Ankyrin repeats (many copies)
Ank PF00023 70 - 93 8.5e-06 Ankyrin repeat
Ank_2 PF12796 141 - 225 5.7e-15 Ankyrin repeats (3 copies)
PGG PF13962 477 - 586 4.9e-31 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 786
Acc36I ACCTGC 1 cut(s) 1357
AccBSI CCGCTC 1 cut(s) 523
AccI GTMKAC 1 cut(s) 1524
AccII CGCG 1 cut(s) 43
AciI CCGC 4 cut(s) 43, 172, 521, 1715
AclWI GGATC 1 cut(s) 575
AcoI YGGCCR 1 cut(s) 139
AcsI RAATTY 3 cut(s) 1100, 1247, 1321
AcuI CTGAAG 1 cut(s) 417
AfaI GTAC 4 cut(s) 878, 1012, 1189, 1260
AfiI CCNNNNNNNGG 4 cut(s) 382, 808, 1517, 2038
AflIII ACRYGT 1 cut(s) 981
AgsI TTSAA 8 cut(s) 20, 65, 748, 988, 1106, 1118, 1218, 1802
AjnI CCWGG 6 cut(s) 7, 379, 807, 1132, 1513, 1527
Alw21I GWGCWC 2 cut(s) 1346, 1933
Alw26I GTCTC 3 cut(s) 705, 1052, 1357
AlwI GGATC 1 cut(s) 575
AoxI GGCC 1 cut(s) 139
ApoI RAATTY 3 cut(s) 1100, 1247, 1321
AspLEI GCGC 2 cut(s) 1173, 1978
AspS9I GGNCC 1 cut(s) 1794
AsuHPI GGTGA 4 cut(s) 835, 938, 1654, 1790
AsuII TTCGAA 1 cut(s) 2046
AvaII GGWCC 1 cut(s) 1794
BanII GRGCYC 1 cut(s) 1346
BauI CACGAG 1 cut(s) 708
BbsI GAAGAC 1 cut(s) 1382
Bbv12I GWGCWC 2 cut(s) 1346, 1933
BbvCI CCTCAGC 1 cut(s) 2037
BccI CCATC 2 cut(s) 1204, 1526
BceAI ACGGC 1 cut(s) 1553
BciT130I CCWGG 6 cut(s) 9, 381, 809, 1134, 1515, 1529
BciVI GTATCC 2 cut(s) 1987, 2029
BclI TGATCA 1 cut(s) 1141
BcoDI GTCTC 3 cut(s) 705, 1052, 1357
BfaI CTAG 9 cut(s) 428, 864, 1080, 1179, 1329, 1407, 1446, 1949, 2054
BfmI CTRYAG 7 cut(s) 412, 432, 852, 1254, 1423, 1731, 1917
BfuAI ACCTGC 1 cut(s) 1357
BfuI GTATCC 2 cut(s) 1987, 2029
BglII AGATCT 1 cut(s) 1419
BmcAI AGTACT 1 cut(s) 1012
Bme1390I CCNGG 6 cut(s) 9, 381, 809, 1134, 1515, 1529
Bme18I GGWCC 1 cut(s) 1794
BmgT120I GGNCC 1 cut(s) 1794
BmiI GGNNCC 2 cut(s) 1371, 1796
BmrFI CCNGG 6 cut(s) 9, 381, 809, 1134, 1515, 1529
BmsI GCATC 2 cut(s) 1656, 2048
BpiI GAAGAC 1 cut(s) 1382
BplI GAGNNNNNCTC 2 cut(s) 66, 98
BpmI CTGGAG 1 cut(s) 1983
Bpu10I CCTNAGC 1 cut(s) 2037
Bpu14I TTCGAA 1 cut(s) 2046
BpuEI CTTGAG 3 cut(s) 617, 683, 1112
Bsa29I ATCGAT 1 cut(s) 450
BsaAI YACGTR 1 cut(s) 550
BsaJI CCNNGG 5 cut(s) 8, 238, 380, 771, 1482
Bsc4I CCNNNNNNNGG 4 cut(s) 382, 808, 1517, 2038
Bse1I ACTGG 4 cut(s) 249, 1195, 1245, 1796
Bse3DI GCAATG 1 cut(s) 1433
BseBI CCWGG 6 cut(s) 9, 381, 809, 1134, 1515, 1529
BseCI ATCGAT 1 cut(s) 450
BseDI CCNNGG 5 cut(s) 8, 238, 380, 771, 1482
BseGI GGATG 3 cut(s) 942, 1537, 2063
BseLI CCNNNNNNNGG 4 cut(s) 382, 808, 1517, 2038
BseMI GCAATG 1 cut(s) 1433
BseMII CTCAG 5 cut(s) 87, 1005, 1047, 1237, 2028
BseNI ACTGG 4 cut(s) 249, 1195, 1245, 1796
BseRI GAGGAG 1 cut(s) 791
BsgI GTGCAG 2 cut(s) 1071, 1842
Bsh1236I CGCG 1 cut(s) 43
BshFI GGCC 1 cut(s) 141
BshVI ATCGAT 1 cut(s) 450
BsiHKAI GWGCWC 2 cut(s) 1346, 1933
BsiSI CCGG 1 cut(s) 1660
BslFI GGGAC 1 cut(s) 1840
BslI CCNNNNNNNGG 4 cut(s) 382, 808, 1517, 2038
BsmAI GTCTC 3 cut(s) 705, 1052, 1357
BsmFI GGGAC 1 cut(s) 1840
BsnI GGCC 1 cut(s) 141
Bsp119I TTCGAA 1 cut(s) 2046
Bsp1286I GDGCHC 2 cut(s) 1346, 1933
Bsp143I GATC 4 cut(s) 580, 970, 1141, 1419
BspACI CCGC 4 cut(s) 43, 172, 521, 1715
BspANI GGCC 1 cut(s) 141
BspCNI CTCAG 5 cut(s) 88, 1006, 1048, 1236, 2029
BspDI ATCGAT 1 cut(s) 450
BspFNI CGCG 1 cut(s) 43
BspHI TCATGA 1 cut(s) 1206
BspLI GGNNCC 2 cut(s) 1371, 1796
BspMAI CTGCAG 3 cut(s) 416, 856, 1921
BspMI ACCTGC 1 cut(s) 1357
BspPI GGATC 1 cut(s) 575
BspT104I TTCGAA 1 cut(s) 2046
BsrBI CCGCTC 1 cut(s) 523
BsrDI GCAATG 1 cut(s) 1433
BsrI ACTGG 4 cut(s) 249, 1195, 1245, 1796
BssECI CCNNGG 5 cut(s) 8, 238, 380, 771, 1482
BssMI GATC 4 cut(s) 580, 970, 1141, 1419
BssSI CACGAG 1 cut(s) 708
BssT1I CCWWGG 2 cut(s) 238, 771
Bst2BI CACGAG 1 cut(s) 708
Bst2UI CCWGG 6 cut(s) 9, 381, 809, 1134, 1515, 1529
Bst6I CTCTTC 1 cut(s) 983
BstBAI YACGTR 1 cut(s) 550
BstBI TTCGAA 1 cut(s) 2046
BstC8I GCNNGC 3 cut(s) 478, 535, 1501
BstDEI CTNAG 7 cut(s) 96, 168, 974, 1014, 1056, 1223, 2037
BstENI CCTNNNNNAGG 1 cut(s) 806
BstF5I GGATG 3 cut(s) 942, 1537, 2063
BstFNI CGCG 1 cut(s) 43
BstHHI GCGC 2 cut(s) 1173, 1978
BstKTI GATC 4 cut(s) 583, 973, 1144, 1422
BstMAI GTCTC 3 cut(s) 705, 1052, 1357
BstMBI GATC 4 cut(s) 580, 970, 1141, 1419
BstMWI GCNNNNNNNGC 3 cut(s) 1170, 1742, 1949
BstNI CCWGG 6 cut(s) 9, 381, 809, 1134, 1515, 1529
BstNSI RCATGY 1 cut(s) 985
BstSCI CCNGG 6 cut(s) 7, 379, 807, 1132, 1513, 1527
BstSFI CTRYAG 7 cut(s) 412, 432, 852, 1254, 1423, 1731, 1917
BstUI CGCG 1 cut(s) 43
BstV2I GAAGAC 1 cut(s) 1382
BstX2I RGATCY 1 cut(s) 1419
BstYI RGATCY 1 cut(s) 1419
Bsu15I ATCGAT 1 cut(s) 450
BsuI GTATCC 2 cut(s) 1987, 2029
BsuRI GGCC 1 cut(s) 141
BsuTUI ATCGAT 1 cut(s) 450
BtgZI GCGATG 1 cut(s) 653
BtsCI GGATG 3 cut(s) 942, 1537, 2063
BtsIMutI CAGTG 3 cut(s) 256, 1238, 1670
BveI ACCTGC 1 cut(s) 1357
Cac8I GCNNGC 3 cut(s) 478, 535, 1501
CciI TCATGA 1 cut(s) 1206
CfoI GCGC 2 cut(s) 1173, 1978
Cfr13I GGNCC 1 cut(s) 1794
ClaI ATCGAT 1 cut(s) 450
CseI GACGC 2 cut(s) 401, 1737
Csp6I GTAC 4 cut(s) 877, 1011, 1188, 1259
CspCI CAANNNNNGTGG 2 cut(s) 612, 647
CviQI GTAC 4 cut(s) 877, 1011, 1188, 1259
DdeI CTNAG 7 cut(s) 96, 168, 974, 1014, 1056, 1223, 2037
DpnI GATC 4 cut(s) 582, 972, 1143, 1421
DpnII GATC 4 cut(s) 580, 970, 1141, 1419
EaeI YGGCCR 1 cut(s) 139
Eam1104I CTCTTC 1 cut(s) 983
EarI CTCTTC 1 cut(s) 983
Ecl136II GAGCTC 1 cut(s) 1344
Eco130I CCWWGG 2 cut(s) 238, 771
Eco24I GRGCYC 1 cut(s) 1346
Eco32I GATATC 1 cut(s) 1812
Eco47I GGWCC 1 cut(s) 1794
Eco53kI GAGCTC 1 cut(s) 1344
Eco57I CTGAAG 1 cut(s) 417
EcoICRI GAGCTC 1 cut(s) 1344
EcoNI CCTNNNNNAGG 1 cut(s) 806
EcoRI GAATTC 1 cut(s) 1247
EcoRII CCWGG 6 cut(s) 7, 379, 807, 1132, 1513, 1527
EcoRV GATATC 1 cut(s) 1812
EcoT14I CCWWGG 2 cut(s) 238, 771
EcoT38I GRGCYC 1 cut(s) 1346
ErhI CCWWGG 2 cut(s) 238, 771
FaqI GGGAC 1 cut(s) 1840
FauNDI CATATG 1 cut(s) 283
FbaI TGATCA 1 cut(s) 1141
FblI GTMKAC 1 cut(s) 1524
FokI GGATG 2 cut(s) 929, 1544
FriOI GRGCYC 1 cut(s) 1346
FspBI CTAG 9 cut(s) 428, 864, 1080, 1179, 1329, 1407, 1446, 1949, 2054
GlaI GCGC 2 cut(s) 1172, 1977
GsuI CTGGAG 1 cut(s) 1983
HaeIII GGCC 1 cut(s) 141
HapII CCGG 1 cut(s) 1660
HgaI GACGC 2 cut(s) 401, 1737
HhaI GCGC 2 cut(s) 1173, 1978
Hin6I GCGC 2 cut(s) 1171, 1976
HinP1I GCGC 2 cut(s) 1171, 1976
HincII GTYRAC 2 cut(s) 502, 1554
HindII GTYRAC 2 cut(s) 502, 1554
HindIII AAGCTT 2 cut(s) 228, 474
HinfI GANTC 6 cut(s) 4, 744, 1052, 1214, 1273, 1357
HpaII CCGG 1 cut(s) 1660
HphI GGTGA 4 cut(s) 835, 938, 1654, 1790
Hpy166II GTNNAC 5 cut(s) 502, 765, 1507, 1525, 1554
Hpy188I TCNGA 9 cut(s) 90, 300, 874, 887, 970, 1057, 1226, 1303, 1374
Hpy188III TCNNGA 7 cut(s) 179, 571, 596, 1207, 1329, 1354, 2000
Hpy8I GTNNAC 5 cut(s) 502, 765, 1507, 1525, 1554
Hpy99I CGWCG 1 cut(s) 851
HpyAV CCTTC 5 cut(s) 434, 490, 1112, 1666, 1775
HpyCH4IV ACGT 2 cut(s) 144, 549
HpyF10VI GCNNNNNNNGC 3 cut(s) 1170, 1742, 1949
HpyF3I CTNAG 7 cut(s) 96, 168, 974, 1014, 1056, 1223, 2037
HpySE526I ACGT 2 cut(s) 144, 549
HspAI GCGC 2 cut(s) 1171, 1976
Ksp22I TGATCA 1 cut(s) 1141
Kzo9I GATC 4 cut(s) 580, 970, 1141, 1419
LmnI GCTCC 2 cut(s) 528, 1349
LweI GCATC 2 cut(s) 1656, 2048
MaeI CTAG 9 cut(s) 428, 864, 1080, 1179, 1329, 1407, 1446, 1949, 2054
MaeII ACGT 2 cut(s) 144, 549
MaeIII GTNAC 6 cut(s) 117, 545, 614, 824, 1486, 1858
MalI GATC 4 cut(s) 582, 972, 1143, 1421
MbiI CCGCTC 1 cut(s) 523
MboI GATC 4 cut(s) 580, 970, 1141, 1419
MboII GAAGA 7 cut(s) 410, 1000, 1256, 1279, 1317, 1387, 2060
MfeI CAATTG 1 cut(s) 24
MflI RGATCY 1 cut(s) 1419
MhlI GDGCHC 2 cut(s) 1346, 1933
MluCI AATT 7 cut(s) 24, 781, 907, 1100, 1158, 1247, 1321
MmeI TCCRAC 1 cut(s) 175
MseI TTAA 7 cut(s) 204, 459, 603, 912, 1028, 1542, 1578
MslI CAYNNNNRTG 2 cut(s) 345, 1619
MspI CCGG 1 cut(s) 1660
MspR9I CCNGG 6 cut(s) 9, 381, 809, 1134, 1515, 1529
MunI CAATTG 1 cut(s) 24
MvaI CCWGG 6 cut(s) 9, 381, 809, 1134, 1515, 1529
MvnI CGCG 1 cut(s) 43
MwoI GCNNNNNNNGC 3 cut(s) 1170, 1742, 1949
NdeI CATATG 1 cut(s) 283
NdeII GATC 4 cut(s) 580, 970, 1141, 1419
NlaIV GGNNCC 2 cut(s) 1371, 1796
NmuCI GTSAC 2 cut(s) 545, 1858
NspI RCATGY 1 cut(s) 985
NspV TTCGAA 1 cut(s) 2046
PagI TCATGA 1 cut(s) 1206
PciI ACATGT 1 cut(s) 981
PfeI GAWTC 6 cut(s) 4, 744, 1052, 1214, 1273, 1357
Ppu21I YACGTR 1 cut(s) 550
PscI ACATGT 1 cut(s) 981
PsiI TTATAA 1 cut(s) 786
Psp124BI GAGCTC 1 cut(s) 1346
Psp6I CCWGG 6 cut(s) 7, 379, 807, 1132, 1513, 1527
PspGI CCWGG 6 cut(s) 7, 379, 807, 1132, 1513, 1527
PspN4I GGNNCC 2 cut(s) 1371, 1796
PspPI GGNCC 1 cut(s) 1794
PstI CTGCAG 3 cut(s) 416, 856, 1921
PsuI RGATCY 1 cut(s) 1419
RsaI GTAC 4 cut(s) 878, 1012, 1189, 1260
RsaNI GTAC 4 cut(s) 877, 1011, 1188, 1259
RseI CAYNNNNRTG 2 cut(s) 345, 1619
SacI GAGCTC 1 cut(s) 1346
SaqAI TTAA 7 cut(s) 204, 459, 603, 912, 1028, 1542, 1578
Sau3AI GATC 4 cut(s) 580, 970, 1141, 1419
Sau96I GGNCC 1 cut(s) 1794
ScaI AGTACT 1 cut(s) 1012
ScrFI CCNGG 6 cut(s) 9, 381, 809, 1134, 1515, 1529
SduI GDGCHC 2 cut(s) 1346, 1933
SfaNI GCATC 2 cut(s) 1656, 2048
SfcI CTRYAG 7 cut(s) 412, 432, 852, 1254, 1423, 1731, 1917
SfuI TTCGAA 1 cut(s) 2046
SinI GGWCC 1 cut(s) 1794
SmiMI CAYNNNNRTG 2 cut(s) 345, 1619
SmlI CTYRAG 3 cut(s) 596, 662, 1091
SmoI CTYRAG 3 cut(s) 596, 662, 1091
Sse9I AATT 7 cut(s) 24, 781, 907, 1100, 1158, 1247, 1321
SsiI CCGC 4 cut(s) 43, 172, 521, 1715
SspMI CTAG 9 cut(s) 428, 864, 1080, 1179, 1329, 1407, 1446, 1949, 2054
SstI GAGCTC 1 cut(s) 1346
StyD4I CCNGG 6 cut(s) 7, 379, 807, 1132, 1513, 1527
StyI CCWWGG 2 cut(s) 238, 771
TaiI ACGT 2 cut(s) 147, 552
TaqI TCGA 4 cut(s) 54, 450, 1355, 2046
TasI AATT 7 cut(s) 24, 781, 907, 1100, 1158, 1247, 1321
TatI WGTACW 4 cut(s) 876, 1010, 1187, 1258
TauI GCSGC 2 cut(s) 175, 523
TfiI GAWTC 6 cut(s) 4, 744, 1052, 1214, 1273, 1357
Tru1I TTAA 7 cut(s) 204, 459, 603, 912, 1028, 1542, 1578
Tru9I TTAA 7 cut(s) 204, 459, 603, 912, 1028, 1542, 1578
TscAI CASTG 3 cut(s) 256, 1245, 1677
TseFI GTSAC 2 cut(s) 545, 1858
Tsp45I GTSAC 2 cut(s) 545, 1858
TspGWI ACGGA 1 cut(s) 181
TspRI CASTG 3 cut(s) 256, 1245, 1677
VpaK11BI GGWCC 1 cut(s) 1794
XagI CCTNNNNNAGG 1 cut(s) 806
XapI RAATTY 3 cut(s) 1100, 1247, 1321
XbaI TCTAGA 1 cut(s) 1328
XceI RCATGY 1 cut(s) 985
XmiI GTMKAC 1 cut(s) 1524
XspI CTAG 9 cut(s) 428, 864, 1080, 1179, 1329, 1407, 1446, 1949, 2054
ZrmI AGTACT 1 cut(s) 1012
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.