FvH4_6g32122

ankyrin repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001058
Physical Location & Seq
Forward (+)
25222607 .. 25224718
2112 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g32122.t1

Sequence Viewer

Length: 1557 bp
ATGCAGAAAACTGGGGAAACAAAGAACTCAGTGTTGCACCTTGCCGCAAGGTTCGGTCACATTGAGCTACTTGCCTTTATCATTGAGGTACTTCCTGACTTGGTTTCTGTCGGAAACATCAAGGGTGAAACTGCGTTACACGAAACTTGTCGCCAAGGGAATACCAATGTCGTAAAGCTGCTGCTAGAGGCCAATCCATATGTGGTTGGTACTCTTAACCATGAAATGCAAACTGCTTTTTACGTAGCCTGCAGTTATGGGCATGTAGAAGTTGTTACGTACATGGCAGACCAACCAGGAGTGTTAGACTCTGAAGAACAGGAAGCTTTGAGTCCTCTTCATGTGTCTATAGCAGAAGGCTACTTTGATATTGTTAGAACTATTCTGAAAGGTTGCCCCAGGTTTGCTTGGAAGGTTGACACTAATGGGTGTCTACCGCTTCATATAGCTTGCGAAAAAGGTCACTTGGAGATATCAAGAACACTGTTGATGATTGATCCAAACTTAGCTCTCGAATTTGACCATTATCGTTATACACCAGTACACTTGGCTGCTATGAATGGTCAGATCAAAATCCTTGAGGAACTTCTTTTGTGCTCTCCCAAATGTTTAGAAAGTCTCACAGGAGATGGAGAAACTGTGTTTCATCCTTCAGTTAGATTCAACAGATACGGTGTGTTTACCTCTTTGTTGTCGGAGCACATCATCAAGAATACTACGGTTGATGTCACTTGTAGAAACCAAAGAGGTCAAACGGCAATGGACATCTTACGCCAGCAAAGAAGAGCGTCGGATATCAGAAACATGTTGAAGAGTTTCGGCGGCAGGACGAGCACTGAGCTGTTGTCACCAAATATTGATGAGATAGATTCTGAGACTACAACAACATCAGAAAGTCCTAGAGTTGTACTTGAGCAAGAATTTGAAATGCTTTTCGAAGGTATCATGCATCCGGGAAGTGATGAATATGACTGTGAAATTGGTATGGGGCGTACCGCTAGAGATGATCAATCAATGGAAGATTTCACTGCAGATGGCCGGCTTAAAGGACAGTCAACAGTTGGCAGAACAACAGCTTCTAAGGTCTTTGCAATATGCAACACAGCAGCATTTTTCATATCTATAAGCATTGTCATTTTCTTGGTCAGCATCATTCCTTTCCAGCGAAAACCACTAATGAAGCTACTGGTGGTTGCTCACAAGGTCATGTGGGTAGCGGTGTCACTGATGGCCACAGCTTATATTGCAGCCATGTGGATCATCATGCCTCACTGTGAAGGTGCAAATTGGACCCTTGAAGTAGTGATATCTGTGTGTGCAGGGACTATGGGATCTGTTTTCTTATTCCTTGGTGTTACATGGACCAGACACTGGTTGATGAAGTTAAAGTGGAGGAAGCAGAGAGGTAAAACTACAGAGGAAAGAGCTCAACAAAAAGCTTTACGAGCACATATAAAAGCACAGAAAAGAACAGAGGATACACCAGAATACAACTCGTCATTTTCCACCAACTCCATTGATGGGAGTTTCGAAGAACTAGGATATCATACGTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_4 Ank_5 PGG

Protein Analysis

519

Amino Acids

58.11

Weight (kDa)

6.0

Isoelectric Point (pI)

36.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 12 - 97 3.7e-13 Ankyrin repeats (3 copies)
Ank_4 PF13637 12 - 62 6.2e-06 Ankyrin repeats (many copies)
Ank_2 PF12796 73 - 131 1.1e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 106 - 168 6e-08 Ankyrin repeats (3 copies)
Ank_2 PF12796 113 - 197 6.2e-12 Ankyrin repeats (3 copies)
PGG PF13962 347 - 420 4.2e-13 Domain of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1371
AccI GTMKAC 1 cut(s) 433
AciI CCGC 5 cut(s) 45, 437, 822, 996, 1217
AclWI GGATC 3 cut(s) 491, 1265, 1339
AcoI YGGCCR 2 cut(s) 1036, 1230
AcsI RAATTY 2 cut(s) 515, 920
AcuI CTGAAG 2 cut(s) 333, 636
AfaI GTAC 7 cut(s) 90, 211, 281, 543, 909, 994, 1553
AfiI CCNNNNNNNGG 2 cut(s) 1371, 1521
AflIII ACRYGT 1 cut(s) 804
AgsI TTSAA 4 cut(s) 664, 811, 926, 1298
AjnI CCWGG 2 cut(s) 295, 398
Alw21I GWGCWC 5 cut(s) 599, 702, 836, 1429, 1450
Alw26I GTCTC 2 cut(s) 623, 869
AlwI GGATC 3 cut(s) 491, 1265, 1339
AlwNI CAGNNNCTG 1 cut(s) 1371
AoxI GGCC 3 cut(s) 189, 1036, 1230
ApeKI GCWGC 5 cut(s) 178, 181, 551, 1106, 1247
ApoI RAATTY 2 cut(s) 515, 920
Asp700I GAANNNNTTC 1 cut(s) 815
AspS9I GGNCC 2 cut(s) 1290, 1362
AsuC2I CCSGG 1 cut(s) 954
AsuHPI GGTGA 2 cut(s) 137, 840
AsuII TTCGAA 2 cut(s) 936, 1530
AvaII GGWCC 2 cut(s) 1290, 1362
BalI TGGCCA 1 cut(s) 1232
BanII GRGCYC 1 cut(s) 1429
Bbv12I GWGCWC 5 cut(s) 599, 702, 836, 1429, 1450
BbvI GCAGC 5 cut(s) 165, 168, 538, 1118, 1259
BccI CCATC 4 cut(s) 623, 1028, 1222, 1514
BceAI ACGGC 1 cut(s) 771
BciT130I CCWGG 2 cut(s) 297, 400
BciVI GTATCC 1 cut(s) 1471
BclI TGATCA 1 cut(s) 1006
BcnI CCSGG 1 cut(s) 954
BcoDI GTCTC 2 cut(s) 623, 869
BfaI CTAG 4 cut(s) 185, 900, 999, 1538
BfmI CTRYAG 4 cut(s) 250, 348, 1029, 1413
BfuI GTATCC 1 cut(s) 1471
BisI GCNGC 7 cut(s) 45, 179, 182, 552, 823, 1107, 1248
BlsI GCNGC 7 cut(s) 46, 180, 183, 553, 824, 1108, 1249
Bme1390I CCNGG 3 cut(s) 297, 400, 954
Bme18I GGWCC 2 cut(s) 1290, 1362
BmgT120I GGNCC 2 cut(s) 1290, 1362
BmiI GGNNCC 1 cut(s) 1292
BmrFI CCNGG 3 cut(s) 297, 400, 954
BmrI ACTGGG 1 cut(s) 21
BmsI GCATC 2 cut(s) 958, 1158
BmuI ACTGGG 1 cut(s) 21
Bpu14I TTCGAA 2 cut(s) 936, 1530
BpuEI CTTGAG 2 cut(s) 599, 932
BpuMI CCSGG 1 cut(s) 954
BsaAI YACGTR 3 cut(s) 244, 279, 1551
BsaBI GATNNNNATC 1 cut(s) 572
BsaJI CCNNGG 3 cut(s) 154, 398, 1348
BsaXI ACNNNNNCTCC 2 cut(s) 624, 654
Bsc4I CCNNNNNNNGG 2 cut(s) 1371, 1521
Bse118I RCCGGY 1 cut(s) 1038
Bse1I ACTGG 4 cut(s) 16, 539, 1191, 1376
Bse3DI GCAATG 1 cut(s) 765
Bse8I GATNNNNATC 1 cut(s) 572
BseBI CCWGG 2 cut(s) 297, 400
BseDI CCNNGG 3 cut(s) 154, 398, 1348
BseGI GGATG 2 cut(s) 646, 949
BseJI GATNNNNATC 1 cut(s) 572
BseLI CCNNNNNNNGG 2 cut(s) 1371, 1521
BseMI GCAATG 1 cut(s) 765
BseMII CTCAG 3 cut(s) 42, 828, 864
BseNI ACTGG 4 cut(s) 16, 539, 1191, 1376
BseXI GCAGC 5 cut(s) 165, 168, 538, 1118, 1259
BsgI GTGCAG 1 cut(s) 1338
BshFI GGCC 3 cut(s) 191, 1038, 1232
BsiHKAI GWGCWC 5 cut(s) 599, 702, 836, 1429, 1450
BsiSI CCGG 2 cut(s) 953, 1039
BslFI GGGAC 1 cut(s) 1336
BslI CCNNNNNNNGG 2 cut(s) 1371, 1521
BsmAI GTCTC 2 cut(s) 623, 869
BsmFI GGGAC 1 cut(s) 1336
BsnI GGCC 3 cut(s) 191, 1038, 1232
Bsp119I TTCGAA 2 cut(s) 936, 1530
Bsp1286I GDGCHC 5 cut(s) 599, 702, 836, 1429, 1450
Bsp143I GATC 5 cut(s) 496, 567, 1006, 1257, 1331
BspACI CCGC 5 cut(s) 45, 437, 822, 996, 1217
BspANI GGCC 3 cut(s) 191, 1038, 1232
BspCNI CTCAG 3 cut(s) 41, 829, 865
BspLI GGNNCC 1 cut(s) 1292
BspMAI CTGCAG 2 cut(s) 254, 1033
BspPI GGATC 3 cut(s) 491, 1265, 1339
BspQI GCTCTTC 1 cut(s) 778
BspT104I TTCGAA 2 cut(s) 936, 1530
BsrDI GCAATG 1 cut(s) 765
BsrFI RCCGGY 1 cut(s) 1038
BsrI ACTGG 4 cut(s) 16, 539, 1191, 1376
BssAI RCCGGY 1 cut(s) 1038
BssECI CCNNGG 3 cut(s) 154, 398, 1348
BssMI GATC 5 cut(s) 496, 567, 1006, 1257, 1331
BssT1I CCWWGG 2 cut(s) 154, 1348
Bst2UI CCWGG 2 cut(s) 297, 400
Bst4CI ACNGT 8 cut(s) 486, 640, 674, 721, 974, 1053, 1060, 1274
Bst6I CTCTTC 3 cut(s) 342, 778, 806
BstBAI YACGTR 3 cut(s) 244, 279, 1551
BstBI TTCGAA 2 cut(s) 936, 1530
BstC8I GCNNGC 4 cut(s) 250, 451, 776, 1040
BstDEI CTNAG 5 cut(s) 28, 505, 837, 873, 1080
BstF5I GGATG 2 cut(s) 646, 949
BstKTI GATC 5 cut(s) 499, 570, 1009, 1260, 1334
BstMAI GTCTC 2 cut(s) 623, 869
BstMBI GATC 5 cut(s) 496, 567, 1006, 1257, 1331
BstMWI GCNNNNNNNGC 3 cut(s) 831, 1244, 1445
BstNI CCWGG 2 cut(s) 297, 400
BstNSI RCATGY 2 cut(s) 266, 808
BstSCI CCNGG 3 cut(s) 295, 398, 952
BstSFI CTRYAG 4 cut(s) 250, 348, 1029, 1413
BstSNI TACGTA 3 cut(s) 244, 279, 1551
BstV1I GCAGC 5 cut(s) 165, 168, 538, 1118, 1259
BstX2I RGATCY 1 cut(s) 1331
BstYI RGATCY 1 cut(s) 1331
BsuI GTATCC 1 cut(s) 1471
BsuRI GGCC 3 cut(s) 191, 1038, 1232
BtsCI GGATG 2 cut(s) 646, 949
BtsI GCAGTG 1 cut(s) 1026
BtsIMutI CAGTG 7 cut(s) 36, 482, 834, 1026, 1223, 1270, 1369
Cac8I GCNNGC 4 cut(s) 250, 451, 776, 1040
CaiI CAGNNNCTG 1 cut(s) 1371
Cfr10I RCCGGY 1 cut(s) 1038
Cfr13I GGNCC 2 cut(s) 1290, 1362
CseI GACGC 1 cut(s) 777
Csp6I GTAC 7 cut(s) 89, 210, 280, 542, 908, 993, 1552
CviQI GTAC 7 cut(s) 89, 210, 280, 542, 908, 993, 1552
DdeI CTNAG 5 cut(s) 28, 505, 837, 873, 1080
DpnI GATC 5 cut(s) 498, 569, 1008, 1259, 1333
DpnII GATC 5 cut(s) 496, 567, 1006, 1257, 1331
EaeI YGGCCR 2 cut(s) 1036, 1230
Eam1104I CTCTTC 3 cut(s) 342, 778, 806
EarI CTCTTC 3 cut(s) 342, 778, 806
Ecl136II GAGCTC 1 cut(s) 1427
Eco105I TACGTA 3 cut(s) 244, 279, 1551
Eco130I CCWWGG 2 cut(s) 154, 1348
Eco24I GRGCYC 1 cut(s) 1429
Eco32I GATATC 4 cut(s) 474, 796, 1308, 1544
Eco47I GGWCC 2 cut(s) 1290, 1362
Eco53kI GAGCTC 1 cut(s) 1427
Eco57I CTGAAG 2 cut(s) 333, 636
EcoICRI GAGCTC 1 cut(s) 1427
EcoRII CCWGG 2 cut(s) 295, 398
EcoRV GATATC 4 cut(s) 474, 796, 1308, 1544
EcoT14I CCWWGG 2 cut(s) 154, 1348
EcoT22I ATGCAT 1 cut(s) 951
EcoT38I GRGCYC 1 cut(s) 1429
ErhI CCWWGG 2 cut(s) 154, 1348
FaqI GGGAC 1 cut(s) 1336
FauNDI CATATG 1 cut(s) 199
FbaI TGATCA 1 cut(s) 1006
FblI GTMKAC 1 cut(s) 433
Fnu4HI GCNGC 7 cut(s) 45, 179, 182, 552, 823, 1107, 1248
FokI GGATG 2 cut(s) 633, 936
FriOI GRGCYC 1 cut(s) 1429
Fsp4HI GCNGC 7 cut(s) 45, 179, 182, 552, 823, 1107, 1248
FspBI CTAG 4 cut(s) 185, 900, 999, 1538
GluI GCNGC 7 cut(s) 45, 179, 182, 552, 823, 1107, 1248
HaeIII GGCC 3 cut(s) 191, 1038, 1232
HapII CCGG 2 cut(s) 953, 1039
HgaI GACGC 1 cut(s) 777
HincII GTYRAC 2 cut(s) 418, 1056
HindII GTYRAC 2 cut(s) 418, 1056
HindIII AAGCTT 2 cut(s) 324, 1437
HinfI GANTC 4 cut(s) 308, 331, 660, 869
HpaII CCGG 2 cut(s) 953, 1039
HphI GGTGA 2 cut(s) 137, 840
Hpy166II GTNNAC 5 cut(s) 418, 434, 544, 681, 1056
Hpy188I TCNGA 9 cut(s) 113, 313, 387, 567, 697, 793, 800, 874, 892
Hpy188III TCNNGA 4 cut(s) 95, 477, 512, 709
Hpy8I GTNNAC 5 cut(s) 418, 434, 544, 681, 1056
Hpy99I CGWCG 1 cut(s) 793
HpyAV CCTTC 5 cut(s) 350, 406, 660, 932, 1271
HpyCH4III ACNGT 8 cut(s) 486, 640, 674, 721, 974, 1053, 1060, 1274
HpyCH4IV ACGT 3 cut(s) 243, 278, 1550
HpyF10VI GCNNNNNNNGC 3 cut(s) 831, 1244, 1445
HpyF3I CTNAG 5 cut(s) 28, 505, 837, 873, 1080
HpySE526I ACGT 3 cut(s) 243, 278, 1550
KroI GCCGGC 1 cut(s) 1038
KroNI GCCGGC 1 cut(s) 1040
Ksp22I TGATCA 1 cut(s) 1006
Kzo9I GATC 5 cut(s) 496, 567, 1006, 1257, 1331
LguI GCTCTTC 1 cut(s) 778
LmnI GCTCC 1 cut(s) 697
Lsp1109I GCAGC 5 cut(s) 165, 168, 538, 1118, 1259
LweI GCATC 2 cut(s) 958, 1158
MaeI CTAG 4 cut(s) 185, 900, 999, 1538
MaeII ACGT 3 cut(s) 243, 278, 1550
MaeIII GTNAC 8 cut(s) 56, 135, 274, 461, 727, 846, 1221, 1354
MalI GATC 5 cut(s) 498, 569, 1008, 1259, 1333
MboI GATC 5 cut(s) 496, 567, 1006, 1257, 1331
MboII GAAGA 6 cut(s) 326, 329, 795, 823, 1031, 1544
MflI RGATCY 1 cut(s) 1331
MhlI GDGCHC 5 cut(s) 599, 702, 836, 1429, 1450
MlsI TGGCCA 1 cut(s) 1232
MluCI AATT 4 cut(s) 515, 920, 978, 1285
MluNI TGGCCA 1 cut(s) 1232
MlyI GAGTC 2 cut(s) 302, 340
MmeI TCCRAC 3 cut(s) 91, 675, 771
Mox20I TGGCCA 1 cut(s) 1232
Mph1103I ATGCAT 1 cut(s) 951
MroNI GCCGGC 1 cut(s) 1038
MroXI GAANNNNTTC 1 cut(s) 815
MscI TGGCCA 1 cut(s) 1232
MseI TTAA 3 cut(s) 216, 1044, 1385
Msp20I TGGCCA 1 cut(s) 1232
MspI CCGG 2 cut(s) 953, 1039
MspR9I CCNGG 3 cut(s) 297, 400, 954
MvaI CCWGG 2 cut(s) 297, 400
MwoI GCNNNNNNNGC 3 cut(s) 831, 1244, 1445
NaeI GCCGGC 1 cut(s) 1040
NciI CCSGG 1 cut(s) 954
NdeI CATATG 1 cut(s) 199
NdeII GATC 5 cut(s) 496, 567, 1006, 1257, 1331
NgoMIV GCCGGC 1 cut(s) 1038
NlaIV GGNNCC 1 cut(s) 1292
NmuCI GTSAC 5 cut(s) 56, 461, 727, 846, 1221
NsiI ATGCAT 1 cut(s) 951
NspI RCATGY 2 cut(s) 266, 808
NspV TTCGAA 2 cut(s) 936, 1530
PciI ACATGT 1 cut(s) 804
PciSI GCTCTTC 1 cut(s) 778
PdiI GCCGGC 1 cut(s) 1040
PdmI GAANNNNTTC 1 cut(s) 815
PfeI GAWTC 2 cut(s) 660, 869
PflMI CCANNNNNTGG 1 cut(s) 1371
PfoI TCCNGGA 1 cut(s) 952
PkrI GCNGC 7 cut(s) 46, 180, 183, 553, 824, 1108, 1249
PleI GAGTC 2 cut(s) 302, 339
PpsI GAGTC 2 cut(s) 302, 339
Ppu21I YACGTR 3 cut(s) 244, 279, 1551
PscI ACATGT 1 cut(s) 804
Psp124BI GAGCTC 1 cut(s) 1429
Psp6I CCWGG 2 cut(s) 295, 398
PspGI CCWGG 2 cut(s) 295, 398
PspN4I GGNNCC 1 cut(s) 1292
PspPI GGNCC 2 cut(s) 1290, 1362
PsrI GAACNNNNNNTAC 2 cut(s) 1462, 1494
PstI CTGCAG 2 cut(s) 254, 1033
PstNI CAGNNNCTG 1 cut(s) 1371
PsuI RGATCY 1 cut(s) 1331
RsaI GTAC 7 cut(s) 90, 211, 281, 543, 909, 994, 1553
RsaNI GTAC 7 cut(s) 89, 210, 280, 542, 908, 993, 1552
SacI GAGCTC 1 cut(s) 1429
SapI GCTCTTC 1 cut(s) 778
SaqAI TTAA 3 cut(s) 216, 1044, 1385
SatI GCNGC 7 cut(s) 45, 179, 182, 552, 823, 1107, 1248
Sau3AI GATC 5 cut(s) 496, 567, 1006, 1257, 1331
Sau96I GGNCC 2 cut(s) 1290, 1362
SchI GAGTC 2 cut(s) 302, 340
ScrFI CCNGG 3 cut(s) 297, 400, 954
SduI GDGCHC 5 cut(s) 599, 702, 836, 1429, 1450
SfaNI GCATC 2 cut(s) 958, 1158
SfcI CTRYAG 4 cut(s) 250, 348, 1029, 1413
SfuI TTCGAA 2 cut(s) 936, 1530
SinI GGWCC 2 cut(s) 1290, 1362
SmlI CTYRAG 2 cut(s) 578, 911
SmoI CTYRAG 2 cut(s) 578, 911
SnaBI TACGTA 3 cut(s) 244, 279, 1551
Sse9I AATT 4 cut(s) 515, 920, 978, 1285
SsiI CCGC 5 cut(s) 45, 437, 822, 996, 1217
SspI AATATT 1 cut(s) 856
SspMI CTAG 4 cut(s) 185, 900, 999, 1538
SstI GAGCTC 1 cut(s) 1429
StyD4I CCNGG 3 cut(s) 295, 398, 952
StyI CCWWGG 2 cut(s) 154, 1348
TaaI ACNGT 8 cut(s) 486, 640, 674, 721, 974, 1053, 1060, 1274
TaiI ACGT 3 cut(s) 246, 281, 1553
TaqI TCGA 3 cut(s) 513, 936, 1530
TaqII GACCGA 1 cut(s) 44
TasI AATT 4 cut(s) 515, 920, 978, 1285
TatI WGTACW 2 cut(s) 541, 907
TauI GCSGC 2 cut(s) 47, 825
TfiI GAWTC 2 cut(s) 660, 869
Tru1I TTAA 3 cut(s) 216, 1044, 1385
Tru9I TTAA 3 cut(s) 216, 1044, 1385
TscAI CASTG 7 cut(s) 36, 489, 841, 1033, 1230, 1277, 1376
TseFI GTSAC 5 cut(s) 56, 461, 727, 846, 1221
TseI GCWGC 5 cut(s) 178, 181, 551, 1106, 1247
Tsp45I GTSAC 5 cut(s) 56, 461, 727, 846, 1221
TspDTI ATGAA 9 cut(s) 237, 329, 431, 572, 635, 978, 1105, 1193, 1394
TspRI CASTG 7 cut(s) 36, 489, 841, 1033, 1230, 1277, 1376
Van91I CCANNNNNTGG 1 cut(s) 1371
VpaK11BI GGWCC 2 cut(s) 1290, 1362
XapI RAATTY 2 cut(s) 515, 920
XceI RCATGY 2 cut(s) 266, 808
XcmI CCANNNNNNNNNTGG 1 cut(s) 199
XmiI GTMKAC 1 cut(s) 433
XmnI GAANNNNTTC 1 cut(s) 815
XspI CTAG 4 cut(s) 185, 900, 999, 1538
Zsp2I ATGCAT 1 cut(s) 951
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.