AT5G60460

Necessary for protein translocation in the endoplasmic reticulum

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0001066
Physical Location & Seq
Reverse (-)
24316833 .. 24318241
1409 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G60460.2

Sequence Viewer

Length: 330 bp
ATGGCGAGAGGCTCTTCCCAATCCCAACAATCAACCTCCACCGGTTCTTCAACCGCCCGGCCCGGTCTGGCCGCTCCTCGTGGTTCCGCCGCCGCTACTGCCGGCATGCGTCGCCGCAGGCTCGGTGGCGGTGGTGGCGTATCCTCAGGCGGCGGATCTGGAGTTGGATCAGGATCGAGCAACATGCTGAGATTCTACACAGATGATGCTCCGGGGTTGAAGATCTCACCGACGGTTGTTCTGATCATGAGTCTCTGTTTCATCGGCTTCGTCACTGCTCTTCACGTCTTTGGAAAACTCTACCTCCACAAATCCGGATCCCAAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03060 ko04141 ko04145 ko05110 map03060 map04141 map04145 map05110
Pfam Domains
Protein Families
Sec61_beta

Protein Analysis

109

Amino Acids

10.87

Weight (kDa)

11.56

Isoelectric Point (pI)

66.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec61_beta PF03911 59 - 98 3.9e-19 Sec61beta family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 74
AccIII TCCGGA 1 cut(s) 314
AciI CCGC 9 cut(s) 54, 72, 87, 90, 93, 115, 129, 150, 153
AclWI GGATC 5 cut(s) 163, 175, 181, 312, 325
AcoI YGGCCR 1 cut(s) 69
AgeI ACCGGT 1 cut(s) 41
AgsI TTSAA 2 cut(s) 51, 220
AjiI CACGTC 1 cut(s) 286
AluBI AGCT 1 cut(s) 326
AluI AGCT 1 cut(s) 326
Alw26I GTCTC 1 cut(s) 257
AlwI GGATC 5 cut(s) 163, 175, 181, 312, 325
Aor13HI TCCGGA 1 cut(s) 314
AoxI GGCC 2 cut(s) 59, 69
AsiGI ACCGGT 1 cut(s) 41
AspS9I GGNCC 1 cut(s) 60
AsuC2I CCSGG 3 cut(s) 58, 63, 213
AsuHPI GGTGA 1 cut(s) 219
AxyI CCTNAGG 1 cut(s) 145
BamHI GGATCC 1 cut(s) 317
BauI CACGAG 1 cut(s) 78
BcgI CGANNNNNNTGC 2 cut(s) 166, 200
BciVI GTATCC 1 cut(s) 151
BclI TGATCA 1 cut(s) 243
BcnI CCSGG 3 cut(s) 58, 63, 213
BcoDI GTCTC 1 cut(s) 257
BfuI GTATCC 1 cut(s) 151
BglII AGATCT 1 cut(s) 222
BisI GCNGC 5 cut(s) 72, 90, 93, 115, 151
BlsI GCNGC 5 cut(s) 73, 91, 94, 116, 152
Bme1390I CCNGG 3 cut(s) 58, 63, 213
BmgBI CACGTC 1 cut(s) 286
BmgT120I GGNCC 1 cut(s) 60
BmiI GGNNCC 2 cut(s) 85, 319
BmrFI CCNGG 3 cut(s) 58, 63, 213
BmsI GCATC 1 cut(s) 196
BpmI CTGGAG 1 cut(s) 180
BpuMI CCSGG 3 cut(s) 58, 63, 213
BsaBI GATNNNNATC 1 cut(s) 172
BsaJI CCNNGG 1 cut(s) 212
BsaWI WCCGGW 2 cut(s) 41, 314
BsaXI ACNNNNNCTCC 2 cut(s) 288, 318
Bse118I RCCGGY 2 cut(s) 41, 101
Bse21I CCTNAGG 1 cut(s) 145
Bse8I GATNNNNATC 1 cut(s) 172
BseAI TCCGGA 1 cut(s) 314
BseDI CCNNGG 1 cut(s) 212
BseJI GATNNNNATC 1 cut(s) 172
BseMII CTCAG 2 cut(s) 159, 179
BseRI GAGGAG 1 cut(s) 66
BshFI GGCC 2 cut(s) 61, 71
BshTI ACCGGT 1 cut(s) 41
BsiSI CCGG 6 cut(s) 42, 58, 63, 102, 212, 315
BsmAI GTCTC 1 cut(s) 257
BsnI GGCC 2 cut(s) 61, 71
Bsp13I TCCGGA 1 cut(s) 314
Bsp143I GATC 6 cut(s) 155, 167, 173, 222, 243, 317
BspACI CCGC 9 cut(s) 54, 72, 87, 90, 93, 115, 129, 150, 153
BspANI GGCC 2 cut(s) 61, 71
BspCNI CTCAG 2 cut(s) 158, 180
BspEI TCCGGA 1 cut(s) 314
BspHI TCATGA 1 cut(s) 246
BspLI GGNNCC 2 cut(s) 85, 319
BspPI GGATC 5 cut(s) 163, 175, 181, 312, 325
BspQI GCTCTTC 2 cut(s) 19, 285
BsrBI CCGCTC 1 cut(s) 74
BsrFI RCCGGY 2 cut(s) 41, 101
BssAI RCCGGY 2 cut(s) 41, 101
BssECI CCNNGG 1 cut(s) 212
BssMI GATC 6 cut(s) 155, 167, 173, 222, 243, 317
BssSI CACGAG 1 cut(s) 78
Bst2BI CACGAG 1 cut(s) 78
Bst4CI ACNGT 1 cut(s) 235
Bst6I CTCTTC 2 cut(s) 19, 285
BstC8I GCNNGC 3 cut(s) 103, 107, 119
BstDEI CTNAG 3 cut(s) 145, 188, 327
BstKTI GATC 6 cut(s) 158, 170, 176, 225, 246, 320
BstMAI GTCTC 1 cut(s) 257
BstMBI GATC 6 cut(s) 155, 167, 173, 222, 243, 317
BstMWI GCNNNNNNNGC 3 cut(s) 98, 111, 135
BstNSI RCATGY 2 cut(s) 109, 187
BstSCI CCNGG 3 cut(s) 56, 61, 211
BstX2I RGATCY 3 cut(s) 155, 222, 317
BstYI RGATCY 3 cut(s) 155, 222, 317
Bsu36I CCTNAGG 1 cut(s) 145
BsuI GTATCC 1 cut(s) 151
BsuRI GGCC 2 cut(s) 61, 71
BtrI CACGTC 1 cut(s) 286
BtsI GCAGTG 1 cut(s) 273
BtsIMutI CAGTG 1 cut(s) 273
Cac8I GCNNGC 3 cut(s) 103, 107, 119
CciI TCATGA 1 cut(s) 246
Cfr10I RCCGGY 2 cut(s) 41, 101
Cfr13I GGNCC 1 cut(s) 60
CseI GACGC 1 cut(s) 98
CspAI ACCGGT 1 cut(s) 41
CviAII CATG 3 cut(s) 106, 184, 247
CviJI RGCY 6 cut(s) 12, 61, 71, 121, 267, 326
CviKI_1 RGCY 6 cut(s) 12, 61, 71, 121, 267, 326
DdeI CTNAG 3 cut(s) 145, 188, 327
DpnI GATC 6 cut(s) 157, 169, 175, 224, 245, 319
DpnII GATC 6 cut(s) 155, 167, 173, 222, 243, 317
EaeI YGGCCR 1 cut(s) 69
Eam1104I CTCTTC 2 cut(s) 19, 285
EarI CTCTTC 2 cut(s) 19, 285
EciI GGCGGA 2 cut(s) 76, 168
Eco81I CCTNAGG 1 cut(s) 145
FaeI CATG 3 cut(s) 109, 187, 250
FaiI YATR 3 cut(s) 107, 185, 248
FatI CATG 3 cut(s) 105, 183, 246
FbaI TGATCA 1 cut(s) 243
Fnu4HI GCNGC 5 cut(s) 72, 90, 93, 115, 151
Fsp4HI GCNGC 5 cut(s) 72, 90, 93, 115, 151
GluI GCNGC 5 cut(s) 72, 90, 93, 115, 151
GsuI CTGGAG 1 cut(s) 180
HaeIII GGCC 2 cut(s) 61, 71
HapII CCGG 6 cut(s) 42, 58, 63, 102, 212, 315
HgaI GACGC 1 cut(s) 98
Hin1II CATG 3 cut(s) 109, 187, 250
HindIII AAGCTT 1 cut(s) 324
HinfI GANTC 2 cut(s) 192, 250
HpaII CCGG 6 cut(s) 42, 58, 63, 102, 212, 315
HphI GGTGA 1 cut(s) 219
Hpy188I TCNGA 1 cut(s) 243
Hpy188III TCNNGA 4 cut(s) 159, 171, 247, 315
Hpy99I CGWCG 2 cut(s) 114, 235
HpyCH4III ACNGT 1 cut(s) 235
HpyCH4IV ACGT 1 cut(s) 285
HpyF10VI GCNNNNNNNGC 3 cut(s) 98, 111, 135
HpyF3I CTNAG 3 cut(s) 145, 188, 327
HpySE526I ACGT 1 cut(s) 285
Hsp92II CATG 3 cut(s) 109, 187, 250
Kpn2I TCCGGA 1 cut(s) 314
KroI GCCGGC 1 cut(s) 101
KroNI GCCGGC 1 cut(s) 103
Ksp22I TGATCA 1 cut(s) 243
Kzo9I GATC 6 cut(s) 155, 167, 173, 222, 243, 317
LguI GCTCTTC 2 cut(s) 19, 285
LmnI GCTCC 2 cut(s) 79, 214
LweI GCATC 1 cut(s) 196
MaeII ACGT 1 cut(s) 285
MaeIII GTNAC 1 cut(s) 271
MalI GATC 6 cut(s) 157, 169, 175, 224, 245, 319
MbiI CCGCTC 1 cut(s) 74
MboI GATC 6 cut(s) 155, 167, 173, 222, 243, 317
MboII GAAGA 4 cut(s) 6, 39, 232, 272
MflI RGATCY 3 cut(s) 155, 222, 317
MlyI GAGTC 1 cut(s) 259
MmeI TCCRAC 1 cut(s) 145
MnlI CCTC 4 cut(s) 46, 87, 154, 314
MroI TCCGGA 1 cut(s) 314
MroNI GCCGGC 1 cut(s) 101
MspI CCGG 6 cut(s) 42, 58, 63, 102, 212, 315
MspR9I CCNGG 3 cut(s) 58, 63, 213
MwoI GCNNNNNNNGC 3 cut(s) 98, 111, 135
NaeI GCCGGC 1 cut(s) 103
NciI CCSGG 3 cut(s) 58, 63, 213
NdeII GATC 6 cut(s) 155, 167, 173, 222, 243, 317
NgoMIV GCCGGC 1 cut(s) 101
NlaIII CATG 3 cut(s) 109, 187, 250
NlaIV GGNNCC 2 cut(s) 85, 319
NmuCI GTSAC 1 cut(s) 271
NspI RCATGY 2 cut(s) 109, 187
PaeI GCATGC 1 cut(s) 109
PagI TCATGA 1 cut(s) 246
PciSI GCTCTTC 2 cut(s) 19, 285
PdiI GCCGGC 1 cut(s) 103
PfeI GAWTC 1 cut(s) 192
PinAI ACCGGT 1 cut(s) 41
PkrI GCNGC 5 cut(s) 73, 91, 94, 116, 152
PleI GAGTC 1 cut(s) 258
PpsI GAGTC 1 cut(s) 258
PspN4I GGNNCC 2 cut(s) 85, 319
PspPI GGNCC 1 cut(s) 60
PsuI RGATCY 3 cut(s) 155, 222, 317
SapI GCTCTTC 2 cut(s) 19, 285
SatI GCNGC 5 cut(s) 72, 90, 93, 115, 151
Sau3AI GATC 6 cut(s) 155, 167, 173, 222, 243, 317
Sau96I GGNCC 1 cut(s) 60
SchI GAGTC 1 cut(s) 259
ScrFI CCNGG 3 cut(s) 58, 63, 213
SetI ASST 4 cut(s) 38, 288, 306, 328
SfaNI GCATC 1 cut(s) 196
SphI GCATGC 1 cut(s) 109
SsiI CCGC 9 cut(s) 54, 72, 87, 90, 93, 115, 129, 150, 153
StyD4I CCNGG 3 cut(s) 56, 61, 211
TaaI ACNGT 1 cut(s) 235
TaiI ACGT 1 cut(s) 288
TaqI TCGA 1 cut(s) 176
TauI GCSGC 5 cut(s) 74, 92, 95, 117, 153
TfiI GAWTC 1 cut(s) 192
TscAI CASTG 1 cut(s) 280
TseFI GTSAC 1 cut(s) 271
Tsp45I GTSAC 1 cut(s) 271
TspDTI ATGAA 1 cut(s) 250
TspRI CASTG 1 cut(s) 280
XceI RCATGY 2 cut(s) 109, 187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.