Rorug04G0086700

Protein transport protein Sec61 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001066
Physical Location & Seq
Reverse (-)
13464717 .. 13466898
2182 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0086700.1

Sequence Viewer

Length: 1104 bp
ATGTTCCACCAAGATGTCCTCACTCACATCCTTCTACACCTCCCCGTCAAGTCTCTCCTCCGCTTCAAATGCGTCTCCAAGCAGTGGCTCTCTGTCATCTCCAGCCCCCAGTTCCACCACCGCCGCCGCCGTCTCGCCTCCTCCGGCATCATCTTGTGTCAAACAACCGATCTCATCCACGACATATCTCTTGCCGGAAGCAACTCCGACCCACCCTTCACATCCCTGAGCTTCATCGACGACTCGGCAGGTGTCAAGATCCTCCAGTCCAGCAATGGCCTGCTCTTGTGCTGCAGCAGCCTTCACAAAGTGGGTATACGCCGCAGCTATTACATCTGCAATCCTTCCACAAAGCAAGTCTTGATTCTTCCTGAAGCAGATTGCGAGTCCAAAACTATTTCTGGAGTTTACTTAGCTTTTGATTCGTCCAAGTCGCCTCACTACCAAGTTGTGTCTGTTCGAAGTTGTTCTTCCCCATCATCATCCAATAGCTATCGCATCGTTACTTACTCTTCAGAGACTCGGGCTTGGAGGCTTTCCGGGTCTCCTTTCGCTGCCCCAGACATGGCGTTTGACAATGGAGTGCTTTGGAATGATGCAATACACTGGATTAGTCCAACTGGGGATTCATTATGTTTTGACATCGATCAAGAACGCCTCGGAACACTGCCTAATCTTCCATCAACTGAGAAATGGAGCAACAGGAGGTTGAGGTATTTCGGCGAGTCCGGTGGCCATTTACACCTAATTGAAGTCTACGGCAGCGCGACTCAATTCCATGTCTTTGAGATGGAGAGAGACTACTCTAGCTGGCTTCCAAAGTACCAAGTTGACATAGCAGCGATTGTTGATGCATTTCCACTGATGGTTTGGAACTACCCTAACGCTAATGATTCTGTTTTTTATGCATTTAGTCTGCTATTTCTCAAAGAAGATGAAGAGGACTCAATTCTGTTGTTGCAAGTACCTGGTGAGTTTCTATCATATAATCTTAGGGATGGGACCTTCAAGAAGCTTGGTCCAATATCCACCACAACAAATATTGCATTTCAGATTGGATGTTTCCATGCATATCAGTTTACAGAGACTTTGGCTAGTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03060 ko04141 ko04145 ko05110 map03060 map04141 map04145 map05110
Pfam Domains
Protein Families
Sec61_beta

Protein Analysis

367

Amino Acids

41.25

Weight (kDa)

6.22

Isoelectric Point (pI)

59.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 5 - 40 5.3e-09 F-box domain
F-box-like PF12937 5 - 42 6.3e-06 F-box-like
FBA_3 PF08268 78 - 229 2.4e-10 F-box associated beta propeller domain
FBA_1 PF07734 80 - 262 9.5e-12 F-box associated beta propeller domain
Beta-prop_KIB1-4 PF03478 81 - 272 2.7e-09 KIB1-4 beta-propeller
b-prop_At3g26010-like PF24750 85 - 290 8.1e-10 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 239
Acc36I ACCTGC 1 cut(s) 239
AccB7I CCANNNNNTGG 1 cut(s) 84
AccI GTMKAC 2 cut(s) 316, 756
AccII CGCG 1 cut(s) 767
AciI CCGC 5 cut(s) 61, 121, 124, 127, 322
AclWI GGATC 1 cut(s) 253
AcoI YGGCCR 1 cut(s) 733
AcuI CTGAAG 2 cut(s) 393, 498
AdeI CACNNNGTG 1 cut(s) 310
AfaI GTAC 2 cut(s) 824, 966
AfiI CCNNNNNNNGG 2 cut(s) 84, 565
AgsI TTSAA 3 cut(s) 67, 752, 1009
AjnI CCWGG 1 cut(s) 967
AjuI GAANNNNNNNTTGG 2 cut(s) 610, 642
AluBI AGCT 6 cut(s) 231, 327, 416, 492, 810, 1015
AluI AGCT 6 cut(s) 231, 327, 416, 492, 810, 1015
Alw26I GTCTC 7 cut(s) 57, 79, 137, 512, 549, 792, 1079
AlwI GGATC 1 cut(s) 253
Ama87I CYCGRG 1 cut(s) 522
AoxI GGCC 2 cut(s) 277, 733
ApeKI GCWGC 7 cut(s) 291, 294, 297, 324, 554, 762, 839
ArsI GACNNNNNNTTYG 2 cut(s) 554, 586
Asp700I GAANNNNTTC 1 cut(s) 466
AspLEI GCGC 1 cut(s) 767
AspS9I GGNCC 2 cut(s) 1002, 1019
AsuC2I CCSGG 1 cut(s) 541
AsuHPI GGTGA 1 cut(s) 983
AsuII TTCGAA 1 cut(s) 460
AvaI CYCGRG 1 cut(s) 522
AvaII GGWCC 2 cut(s) 1002, 1019
BalI TGGCCA 1 cut(s) 735
BbvI GCAGC 7 cut(s) 278, 306, 309, 336, 541, 774, 851
BccI CCATC 5 cut(s) 484, 688, 784, 859, 992
BceAI ACGGC 2 cut(s) 114, 775
BciT130I CCWGG 1 cut(s) 969
BcnI CCSGG 1 cut(s) 541
BcoDI GTCTC 7 cut(s) 57, 79, 137, 512, 549, 792, 1079
BfaI CTAG 2 cut(s) 807, 1095
BfmI CTRYAG 1 cut(s) 292
BfuAI ACCTGC 1 cut(s) 239
Bme1390I CCNGG 2 cut(s) 541, 969
Bme18I GGWCC 2 cut(s) 1002, 1019
BmeT110I CYCGRG 1 cut(s) 522
BmgT120I GGNCC 2 cut(s) 1002, 1019
BmiI GGNNCC 1 cut(s) 1003
BmrFI CCNGG 2 cut(s) 541, 969
BmrI ACTGGG 2 cut(s) 103, 630
BmsI GCATC 4 cut(s) 156, 507, 586, 841
BmuI ACTGGG 2 cut(s) 103, 630
BpmI CTGGAG 3 cut(s) 85, 248, 423
Bpu10I CCTNAGC 1 cut(s) 227
Bpu14I TTCGAA 1 cut(s) 460
BpuMI CCSGG 1 cut(s) 541
Bsa29I ATCGAT 1 cut(s) 645
BsaI GGTCTC 1 cut(s) 549
BsaJI CCNNGG 1 cut(s) 658
BsaWI WCCGGW 1 cut(s) 728
Bsc4I CCNNNNNNNGG 2 cut(s) 84, 565
Bse1I ACTGG 4 cut(s) 109, 265, 611, 625
Bse3DI GCAATG 1 cut(s) 280
BseBI CCWGG 1 cut(s) 969
BseCI ATCGAT 1 cut(s) 645
BseDI CCNNGG 1 cut(s) 658
BseGI GGATG 6 cut(s) 27, 174, 221, 482, 1003, 1064
BseLI CCNNNNNNNGG 2 cut(s) 84, 565
BseMI GCAATG 1 cut(s) 280
BseMII CTCAG 2 cut(s) 218, 678
BseNI ACTGG 4 cut(s) 109, 265, 611, 625
BseRI GAGGAG 2 cut(s) 47, 130
BseXI GCAGC 7 cut(s) 278, 306, 309, 336, 541, 774, 851
Bsh1236I CGCG 1 cut(s) 767
BshFI GGCC 2 cut(s) 279, 735
BshVI ATCGAT 1 cut(s) 645
BsiHKCI CYCGRG 1 cut(s) 522
BsiSI CCGG 4 cut(s) 144, 195, 540, 729
BslFI GGGAC 1 cut(s) 1015
BslI CCNNNNNNNGG 2 cut(s) 84, 565
BsmAI GTCTC 7 cut(s) 57, 79, 137, 512, 549, 792, 1079
BsmBI CGTCTC 2 cut(s) 79, 137
BsmFI GGGAC 1 cut(s) 1015
BsnI GGCC 2 cut(s) 279, 735
Bso31I GGTCTC 1 cut(s) 549
BsoBI CYCGRG 1 cut(s) 522
Bsp119I TTCGAA 1 cut(s) 460
Bsp143I GATC 3 cut(s) 169, 258, 646
BspACI CCGC 5 cut(s) 61, 121, 124, 127, 322
BspANI GGCC 2 cut(s) 279, 735
BspCNI CTCAG 2 cut(s) 219, 679
BspDI ATCGAT 1 cut(s) 645
BspFNI CGCG 1 cut(s) 767
BspLI GGNNCC 1 cut(s) 1003
BspMAI CTGCAG 1 cut(s) 296
BspMI ACCTGC 1 cut(s) 239
BspPI GGATC 1 cut(s) 253
BspT104I TTCGAA 1 cut(s) 460
BspTNI GGTCTC 1 cut(s) 549
BsrDI GCAATG 1 cut(s) 280
BsrI ACTGG 4 cut(s) 109, 265, 611, 625
BssECI CCNNGG 1 cut(s) 658
BssMI GATC 3 cut(s) 169, 258, 646
BssNAI GTATAC 1 cut(s) 317
Bst1107I GTATAC 1 cut(s) 317
Bst2UI CCWGG 1 cut(s) 969
Bst6I CTCTTC 2 cut(s) 517, 933
BstBI TTCGAA 1 cut(s) 460
BstC8I GCNNGC 2 cut(s) 281, 812
BstDEI CTNAG 4 cut(s) 227, 412, 687, 992
BstF5I GGATG 6 cut(s) 27, 174, 221, 482, 1003, 1064
BstFNI CGCG 1 cut(s) 767
BstHHI GCGC 1 cut(s) 767
BstKTI GATC 3 cut(s) 172, 261, 649
BstMAI GTCTC 7 cut(s) 57, 79, 137, 512, 549, 792, 1079
BstMBI GATC 3 cut(s) 169, 258, 646
BstMWI GCNNNNNNNGC 2 cut(s) 69, 297
BstNI CCWGG 1 cut(s) 969
BstSCI CCNGG 2 cut(s) 539, 967
BstSFI CTRYAG 1 cut(s) 292
BstUI CGCG 1 cut(s) 767
BstV1I GCAGC 7 cut(s) 278, 306, 309, 336, 541, 774, 851
BstX2I RGATCY 1 cut(s) 258
BstYI RGATCY 1 cut(s) 258
BstZ17I GTATAC 1 cut(s) 317
Bsu15I ATCGAT 1 cut(s) 645
BsuRI GGCC 2 cut(s) 279, 735
BsuTUI ATCGAT 1 cut(s) 645
BtsCI GGATG 6 cut(s) 27, 174, 221, 482, 1003, 1064
BtsI GCAGTG 2 cut(s) 89, 665
BtsIMutI CAGTG 4 cut(s) 89, 604, 665, 860
BveI ACCTGC 1 cut(s) 239
Cac8I GCNNGC 2 cut(s) 281, 812
CfoI GCGC 1 cut(s) 767
Cfr13I GGNCC 2 cut(s) 1002, 1019
ClaI ATCGAT 1 cut(s) 645
CseI GACGC 1 cut(s) 61
CsiI ACCWGGT 1 cut(s) 967
Csp6I GTAC 2 cut(s) 823, 965
CviAII CATG 3 cut(s) 565, 779, 1067
CviQI GTAC 2 cut(s) 823, 965
DdeI CTNAG 4 cut(s) 227, 412, 687, 992
DpnI GATC 3 cut(s) 171, 260, 648
DpnII GATC 3 cut(s) 169, 258, 646
DraIII CACNNNGTG 1 cut(s) 310
EaeI YGGCCR 1 cut(s) 733
Eam1104I CTCTTC 2 cut(s) 517, 933
EarI CTCTTC 2 cut(s) 517, 933
Eco31I GGTCTC 1 cut(s) 549
Eco47I GGWCC 2 cut(s) 1002, 1019
Eco57I CTGAAG 2 cut(s) 393, 498
Eco88I CYCGRG 1 cut(s) 522
EcoO109I RGGNCCY 1 cut(s) 1002
EcoRII CCWGG 1 cut(s) 967
EcoT22I ATGCAT 3 cut(s) 856, 910, 1072
Esp3I CGTCTC 2 cut(s) 79, 137
FaeI CATG 3 cut(s) 568, 782, 1070
FalI AAGNNNNNCTT 4 cut(s) 344, 376, 454, 486
FaqI GGGAC 1 cut(s) 1015
FatI CATG 3 cut(s) 564, 778, 1066
FblI GTMKAC 2 cut(s) 316, 756
FokI GGATG 6 cut(s) 14, 161, 208, 469, 1010, 1071
FspBI CTAG 2 cut(s) 807, 1095
GlaI GCGC 1 cut(s) 766
GsuI CTGGAG 3 cut(s) 85, 248, 423
HaeIII GGCC 2 cut(s) 279, 735
HapII CCGG 4 cut(s) 144, 195, 540, 729
HgaI GACGC 1 cut(s) 61
HhaI GCGC 1 cut(s) 767
Hin1II CATG 3 cut(s) 568, 782, 1070
Hin6I GCGC 1 cut(s) 765
HinP1I GCGC 1 cut(s) 765
HincII GTYRAC 1 cut(s) 832
HindII GTYRAC 1 cut(s) 832
HindIII AAGCTT 1 cut(s) 1013
HpaII CCGG 4 cut(s) 144, 195, 540, 729
HphI GGTGA 1 cut(s) 983
Hpy166II GTNNAC 5 cut(s) 317, 409, 757, 832, 1080
Hpy188I TCNGA 4 cut(s) 208, 517, 662, 1053
Hpy188III TCNNGA 6 cut(s) 256, 361, 371, 402, 650, 1009
Hpy8I GTNNAC 5 cut(s) 317, 409, 757, 832, 1080
Hpy99I CGWCG 1 cut(s) 242
HpyAV CCTTC 5 cut(s) 41, 226, 311, 354, 1015
HpyCH4V TGCA 8 cut(s) 294, 339, 599, 854, 908, 961, 1046, 1070
HpyF10VI GCNNNNNNNGC 2 cut(s) 69, 297
HpyF3I CTNAG 4 cut(s) 227, 412, 687, 992
Hsp92II CATG 3 cut(s) 568, 782, 1070
HspAI GCGC 1 cut(s) 765
Kzo9I GATC 3 cut(s) 169, 258, 646
LmnI GCTCC 1 cut(s) 696
Lsp1109I GCAGC 7 cut(s) 278, 306, 309, 336, 541, 774, 851
LweI GCATC 4 cut(s) 156, 507, 586, 841
MabI ACCWGGT 1 cut(s) 967
MaeI CTAG 2 cut(s) 807, 1095
MaeIII GTNAC 1 cut(s) 502
MalI GATC 3 cut(s) 171, 260, 648
MboI GATC 3 cut(s) 169, 258, 646
MboII GAAGA 6 cut(s) 359, 462, 504, 668, 944, 950
MflI RGATCY 1 cut(s) 258
MlsI TGGCCA 1 cut(s) 735
MluCI AATT 3 cut(s) 747, 773, 948
MluNI TGGCCA 1 cut(s) 735
MlyI GAGTC 6 cut(s) 236, 395, 514, 734, 763, 938
MmeI TCCRAC 2 cut(s) 231, 641
Mox20I TGGCCA 1 cut(s) 735
Mph1103I ATGCAT 3 cut(s) 856, 910, 1072
MroXI GAANNNNTTC 1 cut(s) 466
MscI TGGCCA 1 cut(s) 735
MslI CAYNNNNRTG 1 cut(s) 12
Msp20I TGGCCA 1 cut(s) 735
MspI CCGG 4 cut(s) 144, 195, 540, 729
MspR9I CCNGG 2 cut(s) 541, 969
MvaI CCWGG 1 cut(s) 969
MvnI CGCG 1 cut(s) 767
MwoI GCNNNNNNNGC 2 cut(s) 69, 297
NciI CCSGG 1 cut(s) 541
NdeII GATC 3 cut(s) 169, 258, 646
NlaIII CATG 3 cut(s) 568, 782, 1070
NlaIV GGNNCC 1 cut(s) 1003
NmeAIII GCCGAG 1 cut(s) 224
NsiI ATGCAT 3 cut(s) 856, 910, 1072
NspV TTCGAA 1 cut(s) 460
PaqCI CACCTGC 1 cut(s) 239
PdmI GAANNNNTTC 1 cut(s) 466
PfeI GAWTC 4 cut(s) 364, 422, 626, 893
PflMI CCANNNNNTGG 1 cut(s) 84
PleI GAGTC 6 cut(s) 236, 394, 514, 733, 763, 938
PpsI GAGTC 6 cut(s) 236, 394, 514, 733, 763, 938
PpuMI RGGWCCY 1 cut(s) 1002
Psp5II RGGWCCY 1 cut(s) 1002
Psp6I CCWGG 1 cut(s) 967
PspGI CCWGG 1 cut(s) 967
PspN4I GGNNCC 1 cut(s) 1003
PspPI GGNCC 2 cut(s) 1002, 1019
PspPPI RGGWCCY 1 cut(s) 1002
PstI CTGCAG 1 cut(s) 296
PsuI RGATCY 1 cut(s) 258
RsaI GTAC 2 cut(s) 824, 966
RsaNI GTAC 2 cut(s) 823, 965
RseI CAYNNNNRTG 1 cut(s) 12
Sau3AI GATC 3 cut(s) 169, 258, 646
Sau96I GGNCC 2 cut(s) 1002, 1019
SchI GAGTC 6 cut(s) 236, 395, 514, 734, 763, 938
ScrFI CCNGG 2 cut(s) 541, 969
SexAI ACCWGGT 1 cut(s) 967
SfaNI GCATC 4 cut(s) 156, 507, 586, 841
SfcI CTRYAG 1 cut(s) 292
SfuI TTCGAA 1 cut(s) 460
SinI GGWCC 2 cut(s) 1002, 1019
SmiMI CAYNNNNRTG 1 cut(s) 12
Sse9I AATT 3 cut(s) 747, 773, 948
SsiI CCGC 5 cut(s) 61, 121, 124, 127, 322
SspI AATATT 1 cut(s) 1042
SspMI CTAG 2 cut(s) 807, 1095
StyD4I CCNGG 2 cut(s) 539, 967
TaqI TCGA 3 cut(s) 237, 460, 645
TasI AATT 3 cut(s) 747, 773, 948
TauI GCSGC 3 cut(s) 126, 129, 324
TfiI GAWTC 4 cut(s) 364, 422, 626, 893
TscAI CASTG 4 cut(s) 89, 611, 672, 867
TseI GCWGC 7 cut(s) 291, 294, 297, 324, 554, 762, 839
TspDTI ATGAA 3 cut(s) 223, 618, 951
TspRI CASTG 4 cut(s) 89, 611, 672, 867
Van91I CCANNNNNTGG 1 cut(s) 84
VpaK11BI GGWCC 2 cut(s) 1002, 1019
XcmI CCANNNNNNNNNTGG 2 cut(s) 272, 867
XmiI GTMKAC 2 cut(s) 316, 756
XmnI GAANNNNTTC 1 cut(s) 466
XspI CTAG 2 cut(s) 807, 1095
Zsp2I ATGCAT 3 cut(s) 856, 910, 1072
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.