Rmu_sc0000528.1_g000028

Necessary for protein translocation in the endoplasmic reticulum

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000528.1
OG0001066
Physical Location & Seq
Reverse (-)
112912 .. 113172
261 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000528.1_g000028.1.cds

Sequence Viewer

Length: 261 bp
atggctcctcgtggttcagctactgccacagctggaatgcgcagccgtcgtatcggtggaggtggcaactccagctccggaggcatgggctgcagtggcggtggctctagtgccggaaacaacatactcaggttctacactgacgacgctcctggcttaaagatctcccctaccgtcatgctagttatgagcatctgcttcatcactgccctccacgtcttcggcaagctctacctccaccgatccatcggcgaagcttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03060 ko04141 ko04145 ko05110 map03060 map04141 map04145 map05110
Pfam Domains
Protein Families
Sec61_beta

Protein Analysis

86

Amino Acids

8.71

Weight (kDa)

10.04

Isoelectric Point (pI)

63.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 41
AccIII TCCGGA 1 cut(s) 77
AciI CCGC 1 cut(s) 99
AclWI GGATC 1 cut(s) 237
AjiI CACGTC 1 cut(s) 217
AjnI CCWGG 1 cut(s) 151
AluBI AGCT 5 cut(s) 20, 32, 75, 229, 257
AluI AGCT 5 cut(s) 20, 32, 75, 229, 257
AlwI GGATC 1 cut(s) 237
AlwNI CAGNNNCTG 1 cut(s) 23
Aor13HI TCCGGA 1 cut(s) 77
ApeKI GCWGC 2 cut(s) 42, 90
AspLEI GCGC 1 cut(s) 42
BauI CACGAG 1 cut(s) 9
BbsI GAAGAC 1 cut(s) 211
BbvI GCAGC 2 cut(s) 54, 77
BccI CCATC 1 cut(s) 254
BceAI ACGGC 1 cut(s) 30
BciT130I CCWGG 1 cut(s) 153
BfaI CTAG 2 cut(s) 108, 182
BfmI CTRYAG 1 cut(s) 91
BglII AGATCT 1 cut(s) 162
BisI GCNGC 2 cut(s) 43, 91
BlsI GCNGC 2 cut(s) 44, 92
Bme1390I CCNGG 1 cut(s) 153
BmgBI CACGTC 1 cut(s) 217
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 153
BmsI GCATC 1 cut(s) 201
BpiI GAAGAC 1 cut(s) 211
BpmI CTGGAG 1 cut(s) 55
BsaWI WCCGGW 1 cut(s) 77
BsaXI ACNNNNNCTCC 2 cut(s) 59, 89
BseAI TCCGGA 1 cut(s) 77
BseBI CCWGG 1 cut(s) 153
BseMII CTCAG 1 cut(s) 142
BseXI GCAGC 2 cut(s) 54, 77
BsiSI CCGG 2 cut(s) 78, 114
BsmI GAATGC 1 cut(s) 42
Bsp13I TCCGGA 1 cut(s) 77
Bsp143I GATC 2 cut(s) 162, 242
BspACI CCGC 1 cut(s) 99
BspCNI CTCAG 1 cut(s) 141
BspEI TCCGGA 1 cut(s) 77
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 1 cut(s) 95
BspPI GGATC 1 cut(s) 237
BssMI GATC 2 cut(s) 162, 242
BssSI CACGAG 1 cut(s) 9
Bst2BI CACGAG 1 cut(s) 9
Bst2UI CCWGG 1 cut(s) 153
Bst4CI ACNGT 1 cut(s) 175
BstAPI GCANNNNNTGC 1 cut(s) 90
BstC8I GCNNGC 1 cut(s) 227
BstDEI CTNAG 1 cut(s) 128
BstHHI GCGC 1 cut(s) 42
BstKTI GATC 2 cut(s) 165, 245
BstMBI GATC 2 cut(s) 162, 242
BstMWI GCNNNNNNNGC 4 cut(s) 72, 81, 90, 96
BstNI CCWGG 1 cut(s) 153
BstSCI CCNGG 1 cut(s) 151
BstSFI CTRYAG 1 cut(s) 91
BstV1I GCAGC 2 cut(s) 54, 77
BstV2I GAAGAC 1 cut(s) 211
BstX2I RGATCY 1 cut(s) 162
BstYI RGATCY 1 cut(s) 162
BtrI CACGTC 1 cut(s) 217
BtsI GCAGTG 2 cut(s) 100, 204
BtsIMutI CAGTG 3 cut(s) 100, 138, 204
Cac8I GCNNGC 1 cut(s) 227
CaiI CAGNNNCTG 1 cut(s) 23
CfoI GCGC 1 cut(s) 42
CseI GACGC 1 cut(s) 155
CviAII CATG 2 cut(s) 85, 178
DdeI CTNAG 1 cut(s) 128
DpnI GATC 2 cut(s) 164, 244
DpnII GATC 2 cut(s) 162, 242
EcoRII CCWGG 1 cut(s) 151
FaeI CATG 2 cut(s) 88, 181
FaiI YATR 4 cut(s) 86, 125, 179, 188
FatI CATG 2 cut(s) 84, 177
Fnu4HI GCNGC 2 cut(s) 43, 91
Fsp4HI GCNGC 2 cut(s) 43, 91
FspBI CTAG 2 cut(s) 108, 182
FspI TGCGCA 1 cut(s) 41
GlaI GCGC 1 cut(s) 41
GluI GCNGC 2 cut(s) 43, 91
GsuI CTGGAG 1 cut(s) 55
HapII CCGG 2 cut(s) 78, 114
HgaI GACGC 1 cut(s) 155
HhaI GCGC 1 cut(s) 42
Hin1II CATG 2 cut(s) 88, 181
Hin6I GCGC 1 cut(s) 40
HinP1I GCGC 1 cut(s) 40
HindIII AAGCTT 1 cut(s) 255
HpaII CCGG 2 cut(s) 78, 114
Hpy188III TCNNGA 1 cut(s) 78
Hpy99I CGWCG 2 cut(s) 51, 149
HpyCH4III ACNGT 1 cut(s) 175
HpyCH4IV ACGT 1 cut(s) 216
HpyCH4V TGCA 1 cut(s) 93
HpyF10VI GCNNNNNNNGC 4 cut(s) 72, 81, 90, 96
HpyF3I CTNAG 1 cut(s) 128
HpySE526I ACGT 1 cut(s) 216
Hsp92II CATG 2 cut(s) 88, 181
HspAI GCGC 1 cut(s) 40
Kpn2I TCCGGA 1 cut(s) 77
Kzo9I GATC 2 cut(s) 162, 242
LmnI GCTCC 3 cut(s) 10, 80, 154
LpnPI CCDG 7 cut(s) 18, 85, 91, 115, 127, 138, 165
Lsp1109I GCAGC 2 cut(s) 54, 77
LweI GCATC 1 cut(s) 201
MaeI CTAG 2 cut(s) 108, 182
MaeII ACGT 1 cut(s) 216
MalI GATC 2 cut(s) 164, 244
MboI GATC 2 cut(s) 162, 242
MboII GAAGA 1 cut(s) 211
MflI RGATCY 1 cut(s) 162
MnlI CCTC 5 cut(s) 18, 53, 74, 221, 245
MroI TCCGGA 1 cut(s) 77
MseI TTAA 2 cut(s) 158, 259
MspA1I CMGCKG 1 cut(s) 32
MspI CCGG 2 cut(s) 78, 114
MspR9I CCNGG 1 cut(s) 153
Mva1269I GAATGC 1 cut(s) 42
MvaI CCWGG 1 cut(s) 153
MwoI GCNNNNNNNGC 4 cut(s) 72, 81, 90, 96
NdeII GATC 2 cut(s) 162, 242
NlaIII CATG 2 cut(s) 88, 181
NlaIV GGNNCC 1 cut(s) 6
NsbI TGCGCA 1 cut(s) 41
PctI GAATGC 1 cut(s) 42
PkrI GCNGC 2 cut(s) 44, 92
Psp6I CCWGG 1 cut(s) 151
PspGI CCWGG 1 cut(s) 151
PspN4I GGNNCC 1 cut(s) 6
PstI CTGCAG 1 cut(s) 95
PstNI CAGNNNCTG 1 cut(s) 23
PsuI RGATCY 1 cut(s) 162
PvuII CAGCTG 1 cut(s) 32
SaqAI TTAA 2 cut(s) 158, 259
SatI GCNGC 2 cut(s) 43, 91
Sau3AI GATC 2 cut(s) 162, 242
ScrFI CCNGG 1 cut(s) 153
SetI ASST 9 cut(s) 22, 34, 64, 77, 134, 219, 231, 237, 259
SfaNI GCATC 1 cut(s) 201
SfcI CTRYAG 1 cut(s) 91
SsiI CCGC 1 cut(s) 99
SspMI CTAG 2 cut(s) 108, 182
StyD4I CCNGG 1 cut(s) 151
TaaI ACNGT 1 cut(s) 175
TaiI ACGT 1 cut(s) 219
Tru1I TTAA 2 cut(s) 158, 259
Tru9I TTAA 2 cut(s) 158, 259
TscAI CASTG 3 cut(s) 100, 145, 211
TseI GCWGC 2 cut(s) 42, 90
TspDTI ATGAA 1 cut(s) 190
TspRI CASTG 3 cut(s) 100, 145, 211
XspI CTAG 2 cut(s) 108, 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.