pycom11g10960

Protein transport protein Sec61 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0001066
Physical Location & Seq
Forward (+)
9424527 .. 9425161
635 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g10960.2

Sequence Viewer

Length: 570 bp
ATGTGTACATTGTTTGCAAATTTAGCATTCCAAACATTACATAATTTCAGTGTCTTTCCCGAGGCGATCCATATTTATTTTCGACATCACAGTTTAACTAACCCAACCATAATTCAGCTTAAAGAAGTAAATACACTCCCTAATCATATTTTTGTCCACCTTAATTACACTCCCTACATTTTCTTCATTTGCTCAATTTCTCTCTCTCTTCGTTGCTCACGCTCTCTTCGTGTTGTCCTAACAATGGCTCGAGGTTCATCTCAGTCGCAAGCGACGTCGTCCTCTGCGACCTCGCGCCCCGGCGTCATGGCTCCGAGGGGCTCGGCTGCCGCGACGGCCGGAATGCGTCGTCGTCGTATTGGAGGTTCCACCAGCTCCGGAAGCATCGGCGGAGGCGGAGGCGGGTCCGGTGCCGGAAACAACATGCTGAGATTCTACACGGACGACGCTCCTGGCTTGAAGATCATCCCGACTGTTGTCCTCGTCATGAGCCTCTGCTTCATCGGCTTCGTCACGGCTCTTCATGTGTTCGGCAAGCTGTACCTCCACCGATCTGGTGGCGGAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03060 ko04141 ko04145 ko05110 map03060 map04141 map04145 map05110
Pfam Domains
Protein Families
Sec61_beta

Protein Analysis

190

Amino Acids

20.2

Weight (kDa)

10.65

Isoelectric Point (pI)

54.13

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 278
AccB1I GGYRCC 1 cut(s) 410
AccII CGCG 2 cut(s) 295, 332
AccIII TCCGGA 1 cut(s) 377
AciI CCGC 5 cut(s) 330, 390, 396, 402, 561
AclWI GGATC 1 cut(s) 61
AcoI YGGCCR 1 cut(s) 336
AcsI RAATTY 1 cut(s) 19
AcyI GRCGYC 2 cut(s) 275, 303
AfaI GTAC 2 cut(s) 7, 542
AfiI CCNNNNNNNGG 1 cut(s) 244
AgsI TTSAA 1 cut(s) 460
AjnI CCWGG 1 cut(s) 451
AluBI AGCT 4 cut(s) 118, 375, 538, 566
AluI AGCT 4 cut(s) 118, 375, 538, 566
AlwI GGATC 1 cut(s) 61
Ama87I CYCGRG 2 cut(s) 59, 249
Aor13HI TCCGGA 1 cut(s) 377
AoxI GGCC 1 cut(s) 336
ApeKI GCWGC 1 cut(s) 326
ApoI RAATTY 1 cut(s) 19
AspLEI GCGC 1 cut(s) 297
AspS9I GGNCC 1 cut(s) 405
AsuC2I CCSGG 1 cut(s) 300
AvaI CYCGRG 2 cut(s) 59, 249
AvaII GGWCC 1 cut(s) 405
BanI GGYRCC 1 cut(s) 410
BanII GRGCYC 1 cut(s) 323
BbvI GCAGC 1 cut(s) 313
BceAI ACGGC 2 cut(s) 351, 531
BciT130I CCWGG 1 cut(s) 453
BcnI CCSGG 1 cut(s) 300
BglI GCCNNNNNGGC 1 cut(s) 335
BisI GCNGC 2 cut(s) 327, 330
BlsI GCNGC 2 cut(s) 328, 331
Bme1390I CCNGG 2 cut(s) 300, 453
Bme18I GGWCC 1 cut(s) 405
BmeT110I CYCGRG 2 cut(s) 59, 249
BmgT120I GGNCC 1 cut(s) 405
BmiI GGNNCC 4 cut(s) 312, 367, 406, 412
BmrFI CCNGG 2 cut(s) 300, 453
BmsI GCATC 1 cut(s) 393
BoxI GACNNNNGTC 1 cut(s) 476
BpuMI CCSGG 1 cut(s) 300
BsaHI GRCGYC 2 cut(s) 275, 303
BsaJI CCNNGG 3 cut(s) 60, 298, 314
BsaWI WCCGGW 2 cut(s) 377, 407
Bsc4I CCNNNNNNNGG 1 cut(s) 244
BseAI TCCGGA 1 cut(s) 377
BseBI CCWGG 1 cut(s) 453
BseDI CCNNGG 3 cut(s) 60, 298, 314
BseGI GGATG 1 cut(s) 465
BseLI CCNNNNNNNGG 1 cut(s) 244
BseMII CTCAG 2 cut(s) 275, 419
BseX3I CGGCCG 1 cut(s) 336
BseXI GCAGC 1 cut(s) 313
Bsh1236I CGCG 2 cut(s) 295, 332
Bsh1285I CGRYCG 1 cut(s) 339
BshFI GGCC 1 cut(s) 338
BshNI GGYRCC 1 cut(s) 410
BsiEI CGRYCG 1 cut(s) 339
BsiHKCI CYCGRG 2 cut(s) 59, 249
BsiSI CCGG 5 cut(s) 300, 339, 378, 408, 414
BslI CCNNNNNNNGG 1 cut(s) 244
BsmI GAATGC 2 cut(s) 26, 348
BsnI GGCC 1 cut(s) 338
BsoBI CYCGRG 2 cut(s) 59, 249
Bsp1286I GDGCHC 1 cut(s) 323
Bsp13I TCCGGA 1 cut(s) 377
Bsp1407I TGTACA 1 cut(s) 5
Bsp143I GATC 3 cut(s) 66, 462, 551
BspACI CCGC 5 cut(s) 330, 390, 396, 402, 561
BspANI GGCC 1 cut(s) 338
BspCNI CTCAG 2 cut(s) 274, 420
BspEI TCCGGA 1 cut(s) 377
BspFNI CGCG 2 cut(s) 295, 332
BspHI TCATGA 1 cut(s) 486
BspLI GGNNCC 4 cut(s) 312, 367, 406, 412
BspPI GGATC 1 cut(s) 61
BspQI GCTCTTC 1 cut(s) 525
BspT107I GGYRCC 1 cut(s) 410
BsrGI TGTACA 1 cut(s) 5
BssECI CCNNGG 3 cut(s) 60, 298, 314
BssMI GATC 3 cut(s) 66, 462, 551
BssNI GRCGYC 2 cut(s) 275, 303
Bst2UI CCWGG 1 cut(s) 453
Bst4CI ACNGT 2 cut(s) 92, 475
Bst6I CTCTTC 3 cut(s) 213, 231, 525
BstACI GRCGYC 2 cut(s) 275, 303
BstAUI TGTACA 1 cut(s) 5
BstC8I GCNNGC 2 cut(s) 270, 536
BstDEI CTNAG 2 cut(s) 261, 428
BstF5I GGATG 1 cut(s) 465
BstFNI CGCG 2 cut(s) 295, 332
BstHHI GCGC 1 cut(s) 297
BstKTI GATC 3 cut(s) 69, 465, 554
BstMBI GATC 3 cut(s) 66, 462, 551
BstMCI CGRYCG 1 cut(s) 339
BstMWI GCNNNNNNNGC 4 cut(s) 23, 335, 381, 504
BstNI CCWGG 1 cut(s) 453
BstNSI RCATGY 1 cut(s) 427
BstPAI GACNNNNGTC 1 cut(s) 476
BstSCI CCNGG 2 cut(s) 298, 451
BstUI CGCG 2 cut(s) 295, 332
BstV1I GCAGC 1 cut(s) 313
BstXI CCANNNNNNTGG 1 cut(s) 554
BstZI CGGCCG 1 cut(s) 336
BsuRI GGCC 1 cut(s) 338
BtsCI GGATG 1 cut(s) 465
BtsIMutI CAGTG 1 cut(s) 55
Cac8I GCNNGC 2 cut(s) 270, 536
CciI TCATGA 1 cut(s) 486
CfoI GCGC 1 cut(s) 297
Cfr13I GGNCC 1 cut(s) 405
CseI GACGC 3 cut(s) 292, 335, 455
Csp6I GTAC 2 cut(s) 6, 541
CviAII CATG 4 cut(s) 307, 424, 487, 524
CviQI GTAC 2 cut(s) 6, 541
DdeI CTNAG 2 cut(s) 261, 428
DpnI GATC 3 cut(s) 68, 464, 553
DpnII GATC 3 cut(s) 66, 462, 551
EaeI YGGCCR 1 cut(s) 336
EagI CGGCCG 1 cut(s) 336
Eam1104I CTCTTC 3 cut(s) 213, 231, 525
EarI CTCTTC 3 cut(s) 213, 231, 525
EciI GGCGGA 2 cut(s) 405, 411
EclXI CGGCCG 1 cut(s) 336
Eco24I GRGCYC 1 cut(s) 323
Eco47I GGWCC 1 cut(s) 405
Eco52I CGGCCG 1 cut(s) 336
Eco88I CYCGRG 2 cut(s) 59, 249
EcoRII CCWGG 1 cut(s) 451
EcoT38I GRGCYC 1 cut(s) 323
FaeI CATG 4 cut(s) 310, 427, 490, 527
FaiI YATR 8 cut(s) 42, 72, 110, 147, 308, 425, 488, 525
FatI CATG 4 cut(s) 306, 423, 486, 523
FauI CCCGC 1 cut(s) 395
Fnu4HI GCNGC 2 cut(s) 327, 330
FokI GGATG 1 cut(s) 452
FriOI GRGCYC 1 cut(s) 323
Fsp4HI GCNGC 2 cut(s) 327, 330
GlaI GCGC 1 cut(s) 296
GluI GCNGC 2 cut(s) 327, 330
HaeIII GGCC 1 cut(s) 338
HapII CCGG 5 cut(s) 300, 339, 378, 408, 414
HgaI GACGC 3 cut(s) 292, 335, 455
HhaI GCGC 1 cut(s) 297
Hin1I GRCGYC 2 cut(s) 275, 303
Hin1II CATG 4 cut(s) 310, 427, 490, 527
Hin6I GCGC 1 cut(s) 295
HinP1I GCGC 1 cut(s) 295
HinfI GANTC 1 cut(s) 432
HpaII CCGG 5 cut(s) 300, 339, 378, 408, 414
Hpy166II GTNNAC 2 cut(s) 6, 157
Hpy188I TCNGA 1 cut(s) 315
Hpy188III TCNNGA 4 cut(s) 59, 378, 469, 487
Hpy8I GTNNAC 2 cut(s) 6, 157
Hpy99I CGWCG 7 cut(s) 277, 280, 337, 351, 354, 357, 449
HpyCH4III ACNGT 2 cut(s) 92, 475
HpyCH4IV ACGT 1 cut(s) 275
HpyCH4V TGCA 1 cut(s) 17
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 335, 381, 504
HpyF3I CTNAG 2 cut(s) 261, 428
HpySE526I ACGT 1 cut(s) 275
Hsp92I GRCGYC 2 cut(s) 275, 303
Hsp92II CATG 4 cut(s) 310, 427, 490, 527
HspAI GCGC 1 cut(s) 295
Kpn2I TCCGGA 1 cut(s) 377
Kzo9I GATC 3 cut(s) 66, 462, 551
LguI GCTCTTC 1 cut(s) 525
LmnI GCTCC 4 cut(s) 316, 380, 454, 563
LpnPI CCDG 9 cut(s) 313, 352, 385, 391, 421, 427, 438, 465, 540
Lsp1109I GCAGC 1 cut(s) 313
LweI GCATC 1 cut(s) 393
MaeII ACGT 1 cut(s) 275
MaeIII GTNAC 1 cut(s) 511
MalI GATC 3 cut(s) 68, 464, 553
MboI GATC 3 cut(s) 66, 462, 551
MboII GAAGA 5 cut(s) 175, 200, 218, 472, 512
MhlI GDGCHC 1 cut(s) 323
MluCI AATT 5 cut(s) 19, 43, 111, 163, 195
MroI TCCGGA 1 cut(s) 377
MseI TTAA 3 cut(s) 95, 120, 162
MspI CCGG 5 cut(s) 300, 339, 378, 408, 414
MspR9I CCNGG 2 cut(s) 300, 453
Mva1269I GAATGC 2 cut(s) 26, 348
MvaI CCWGG 1 cut(s) 453
MvnI CGCG 2 cut(s) 295, 332
MwoI GCNNNNNNNGC 4 cut(s) 23, 335, 381, 504
NciI CCSGG 1 cut(s) 300
NdeII GATC 3 cut(s) 66, 462, 551
NlaIII CATG 4 cut(s) 310, 427, 490, 527
NlaIV GGNNCC 4 cut(s) 312, 367, 406, 412
NmeAIII GCCGAG 1 cut(s) 302
NmuCI GTSAC 1 cut(s) 511
NspI RCATGY 1 cut(s) 427
PaeR7I CTCGAG 1 cut(s) 249
PagI TCATGA 1 cut(s) 486
PciSI GCTCTTC 1 cut(s) 525
PcsI WCGNNNNNNNCGW 4 cut(s) 226, 272, 284, 329
PctI GAATGC 2 cut(s) 26, 348
PfeI GAWTC 1 cut(s) 432
PflFI GACNNNGTC 1 cut(s) 277
PkrI GCNGC 2 cut(s) 328, 331
PshAI GACNNNNGTC 1 cut(s) 476
Psp6I CCWGG 1 cut(s) 451
PspGI CCWGG 1 cut(s) 451
PspN4I GGNNCC 4 cut(s) 312, 367, 406, 412
PspPI GGNCC 1 cut(s) 405
PspXI VCTCGAGB 1 cut(s) 249
PsrI GAACNNNNNNTAC 2 cut(s) 349, 381
PsyI GACNNNGTC 1 cut(s) 277
RsaI GTAC 2 cut(s) 7, 542
RsaNI GTAC 2 cut(s) 6, 541
SapI GCTCTTC 1 cut(s) 525
SaqAI TTAA 3 cut(s) 95, 120, 162
SatI GCNGC 2 cut(s) 327, 330
Sau3AI GATC 3 cut(s) 66, 462, 551
Sau96I GGNCC 1 cut(s) 405
ScrFI CCNGG 2 cut(s) 300, 453
SduI GDGCHC 1 cut(s) 323
SfaNI GCATC 1 cut(s) 393
Sfr274I CTCGAG 1 cut(s) 249
SinI GGWCC 1 cut(s) 405
SlaI CTCGAG 1 cut(s) 249
SmlI CTYRAG 1 cut(s) 249
SmoI CTYRAG 1 cut(s) 249
Sse9I AATT 5 cut(s) 19, 43, 111, 163, 195
SsiI CCGC 5 cut(s) 330, 390, 396, 402, 561
StyD4I CCNGG 2 cut(s) 298, 451
TaaI ACNGT 2 cut(s) 92, 475
TaiI ACGT 1 cut(s) 278
TaqI TCGA 2 cut(s) 82, 250
TasI AATT 5 cut(s) 19, 43, 111, 163, 195
TatI WGTACW 1 cut(s) 5
TauI GCSGC 1 cut(s) 332
TfiI GAWTC 1 cut(s) 432
Tru1I TTAA 3 cut(s) 95, 120, 162
Tru9I TTAA 3 cut(s) 95, 120, 162
TscAI CASTG 1 cut(s) 55
TseFI GTSAC 1 cut(s) 511
TseI GCWGC 1 cut(s) 326
Tsp45I GTSAC 1 cut(s) 511
TspDTI ATGAA 4 cut(s) 175, 246, 490, 512
TspGWI ACGGA 1 cut(s) 455
TspRI CASTG 1 cut(s) 55
Tth111I GACNNNGTC 1 cut(s) 277
VpaK11BI GGWCC 1 cut(s) 405
XapI RAATTY 1 cut(s) 19
XceI RCATGY 1 cut(s) 427
XcmI CCANNNNNNNNNTGG 1 cut(s) 554
XhoI CTCGAG 1 cut(s) 249
ZraI GACGTC 1 cut(s) 276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.