RchiOBHm_Chr5g0081371

Necessary for protein translocation in the endoplasmic reticulum

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001066
Physical Location & Seq
Reverse (-)
87336927 .. 87337259
333 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35562

Sequence Viewer

Length: 333 bp
ATGGCTCAAGGCTCATCTCAGTCCCAAGCGACGTCGTCTTCCACGACCTCACACCTCAGCGTCATGGCTCCTGGTGGTTCAGTTGTTGCCACAACTGGAATGCGCAGCCGTCGTATCGATGAAGGGGGCAACTCCAGCTCCGGAGGCATGGGCGGCAGCGACGGTGGCTTTGGTGCAAAAAACAACATACTCAGGTTCTACACCGACGACGCTCCTGGCTTGAAGATCTCCCCCACCGTCATGCTCTTCATGAGCCTCTACTTCATCGGCTTTGTCACTACCCTCCACGTATTCAGCAAGCTCTACCTCCACCGATCCACCGGCGGAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03060 ko04141 ko04145 ko05110 map03060 map04141 map04145 map05110
Pfam Domains
Protein Families
Sec61_beta

Protein Analysis

110

Amino Acids

11.36

Weight (kDa)

9.69

Isoelectric Point (pI)

63.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec61_beta PF03911 61 - 98 3.6e-14 Sec61beta family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 35
Acc16I TGCGCA 1 cut(s) 104
AccIII TCCGGA 1 cut(s) 140
AciI CCGC 2 cut(s) 153, 324
AclWI GGATC 1 cut(s) 309
AcyI GRCGYC 1 cut(s) 32
AgsI TTSAA 1 cut(s) 223
AjnI CCWGG 2 cut(s) 70, 214
AluBI AGCT 3 cut(s) 138, 301, 329
AluI AGCT 3 cut(s) 138, 301, 329
AlwI GGATC 1 cut(s) 309
Aor13HI TCCGGA 1 cut(s) 140
ApeKI GCWGC 2 cut(s) 105, 156
ArsI GACNNNNNNTTYG 2 cut(s) 152, 184
AspLEI GCGC 1 cut(s) 105
BbsI GAAGAC 1 cut(s) 30
BbvCI CCTCAGC 1 cut(s) 56
BbvI GCAGC 2 cut(s) 117, 168
BceAI ACGGC 1 cut(s) 93
BciT130I CCWGG 2 cut(s) 72, 216
BglII AGATCT 1 cut(s) 225
BisI GCNGC 3 cut(s) 106, 154, 157
BlsI GCNGC 3 cut(s) 107, 155, 158
Bme1390I CCNGG 2 cut(s) 72, 216
BmiI GGNNCC 1 cut(s) 69
BmrFI CCNGG 2 cut(s) 72, 216
BpiI GAAGAC 1 cut(s) 30
BpmI CTGGAG 1 cut(s) 118
Bpu10I CCTNAGC 1 cut(s) 56
Bsa29I ATCGAT 1 cut(s) 117
BsaAI YACGTR 1 cut(s) 289
BsaHI GRCGYC 1 cut(s) 32
BsaWI WCCGGW 1 cut(s) 140
BsaXI ACNNNNNCTCC 2 cut(s) 122, 152
Bse118I RCCGGY 1 cut(s) 320
Bse1I ACTGG 1 cut(s) 100
BseAI TCCGGA 1 cut(s) 140
BseBI CCWGG 2 cut(s) 72, 216
BseCI ATCGAT 1 cut(s) 117
BseMII CTCAG 3 cut(s) 32, 70, 205
BseNI ACTGG 1 cut(s) 100
BseXI GCAGC 2 cut(s) 117, 168
BshVI ATCGAT 1 cut(s) 117
BsiSI CCGG 2 cut(s) 141, 321
BslFI GGGAC 1 cut(s) 7
BsmFI GGGAC 1 cut(s) 7
BsmI GAATGC 1 cut(s) 105
Bsp13I TCCGGA 1 cut(s) 140
Bsp143I GATC 2 cut(s) 225, 314
BspACI CCGC 2 cut(s) 153, 324
BspCNI CTCAG 3 cut(s) 31, 69, 204
BspDI ATCGAT 1 cut(s) 117
BspEI TCCGGA 1 cut(s) 140
BspHI TCATGA 1 cut(s) 249
BspLI GGNNCC 1 cut(s) 69
BspPI GGATC 1 cut(s) 309
BspQI GCTCTTC 1 cut(s) 251
BsrFI RCCGGY 1 cut(s) 320
BsrI ACTGG 1 cut(s) 100
BssAI RCCGGY 1 cut(s) 320
BssMI GATC 2 cut(s) 225, 314
BssNI GRCGYC 1 cut(s) 32
Bst2UI CCWGG 2 cut(s) 72, 216
Bst4CI ACNGT 2 cut(s) 164, 238
Bst6I CTCTTC 1 cut(s) 251
BstACI GRCGYC 1 cut(s) 32
BstBAI YACGTR 1 cut(s) 289
BstC8I GCNNGC 1 cut(s) 299
BstDEI CTNAG 3 cut(s) 18, 56, 191
BstHHI GCGC 1 cut(s) 105
BstKTI GATC 2 cut(s) 228, 317
BstMBI GATC 2 cut(s) 225, 314
BstMWI GCNNNNNNNGC 4 cut(s) 135, 144, 153, 165
BstNI CCWGG 2 cut(s) 72, 216
BstSCI CCNGG 2 cut(s) 70, 214
BstV1I GCAGC 2 cut(s) 117, 168
BstV2I GAAGAC 1 cut(s) 30
BstX2I RGATCY 1 cut(s) 225
BstYI RGATCY 1 cut(s) 225
Bsu15I ATCGAT 1 cut(s) 117
BsuTUI ATCGAT 1 cut(s) 117
Cac8I GCNNGC 1 cut(s) 299
CciI TCATGA 1 cut(s) 249
CfoI GCGC 1 cut(s) 105
Cfr10I RCCGGY 1 cut(s) 320
ClaI ATCGAT 1 cut(s) 117
CseI GACGC 2 cut(s) 49, 218
CviAII CATG 4 cut(s) 64, 148, 241, 250
DdeI CTNAG 3 cut(s) 18, 56, 191
DpnI GATC 2 cut(s) 227, 316
DpnII GATC 2 cut(s) 225, 314
Eam1104I CTCTTC 1 cut(s) 251
EarI CTCTTC 1 cut(s) 251
EcoRII CCWGG 2 cut(s) 70, 214
FaeI CATG 4 cut(s) 67, 151, 244, 253
FaiI YATR 5 cut(s) 65, 149, 188, 242, 251
FaqI GGGAC 1 cut(s) 7
FatI CATG 4 cut(s) 63, 147, 240, 249
Fnu4HI GCNGC 3 cut(s) 106, 154, 157
Fsp4HI GCNGC 3 cut(s) 106, 154, 157
FspI TGCGCA 1 cut(s) 104
GlaI GCGC 1 cut(s) 104
GluI GCNGC 3 cut(s) 106, 154, 157
GsuI CTGGAG 1 cut(s) 118
HapII CCGG 2 cut(s) 141, 321
HgaI GACGC 2 cut(s) 49, 218
HhaI GCGC 1 cut(s) 105
Hin1I GRCGYC 1 cut(s) 32
Hin1II CATG 4 cut(s) 67, 151, 244, 253
Hin6I GCGC 1 cut(s) 103
HinP1I GCGC 1 cut(s) 103
HpaII CCGG 2 cut(s) 141, 321
Hpy188III TCNNGA 2 cut(s) 141, 250
Hpy99I CGWCG 6 cut(s) 34, 37, 114, 164, 209, 212
HpyAV CCTTC 1 cut(s) 116
HpyCH4III ACNGT 2 cut(s) 164, 238
HpyCH4IV ACGT 2 cut(s) 32, 288
HpyCH4V TGCA 1 cut(s) 176
HpyF10VI GCNNNNNNNGC 4 cut(s) 135, 144, 153, 165
HpyF3I CTNAG 3 cut(s) 18, 56, 191
HpySE526I ACGT 2 cut(s) 32, 288
Hsp92I GRCGYC 1 cut(s) 32
Hsp92II CATG 4 cut(s) 67, 151, 244, 253
HspAI GCGC 1 cut(s) 103
Kpn2I TCCGGA 1 cut(s) 140
Kzo9I GATC 2 cut(s) 225, 314
LguI GCTCTTC 1 cut(s) 251
LmnI GCTCC 4 cut(s) 73, 143, 217, 326
LpnPI CCDG 8 cut(s) 57, 81, 84, 148, 154, 178, 201, 228
Lsp1109I GCAGC 2 cut(s) 117, 168
MaeII ACGT 2 cut(s) 32, 288
MaeIII GTNAC 1 cut(s) 274
MalI GATC 2 cut(s) 227, 316
MboI GATC 2 cut(s) 225, 314
MboII GAAGA 3 cut(s) 30, 235, 238
MflI RGATCY 1 cut(s) 225
MnlI CCTC 6 cut(s) 58, 65, 137, 266, 293, 317
MroI TCCGGA 1 cut(s) 140
MseI TTAA 1 cut(s) 331
MslI CAYNNNNRTG 1 cut(s) 239
MspI CCGG 2 cut(s) 141, 321
MspR9I CCNGG 2 cut(s) 72, 216
Mva1269I GAATGC 1 cut(s) 105
MvaI CCWGG 2 cut(s) 72, 216
MwoI GCNNNNNNNGC 4 cut(s) 135, 144, 153, 165
NdeII GATC 2 cut(s) 225, 314
NlaIII CATG 4 cut(s) 67, 151, 244, 253
NlaIV GGNNCC 1 cut(s) 69
NmuCI GTSAC 1 cut(s) 274
NsbI TGCGCA 1 cut(s) 104
PagI TCATGA 1 cut(s) 249
PciSI GCTCTTC 1 cut(s) 251
PcsI WCGNNNNNNNCGW 1 cut(s) 41
PctI GAATGC 1 cut(s) 105
PflFI GACNNNGTC 1 cut(s) 34
PkrI GCNGC 3 cut(s) 107, 155, 158
Ppu21I YACGTR 1 cut(s) 289
Psp6I CCWGG 2 cut(s) 70, 214
PspGI CCWGG 2 cut(s) 70, 214
PspN4I GGNNCC 1 cut(s) 69
PsuI RGATCY 1 cut(s) 225
PsyI GACNNNGTC 1 cut(s) 34
RseI CAYNNNNRTG 1 cut(s) 239
SapI GCTCTTC 1 cut(s) 251
SaqAI TTAA 1 cut(s) 331
SatI GCNGC 3 cut(s) 106, 154, 157
Sau3AI GATC 2 cut(s) 225, 314
ScrFI CCNGG 2 cut(s) 72, 216
SetI ASST 9 cut(s) 35, 50, 57, 140, 197, 291, 303, 309, 331
SgrAI CRCCGGYG 1 cut(s) 320
SmiMI CAYNNNNRTG 1 cut(s) 239
SmlI CTYRAG 1 cut(s) 6
SmoI CTYRAG 1 cut(s) 6
SsiI CCGC 2 cut(s) 153, 324
StyD4I CCNGG 2 cut(s) 70, 214
TaaI ACNGT 2 cut(s) 164, 238
TaiI ACGT 2 cut(s) 35, 291
TaqI TCGA 1 cut(s) 117
TauI GCSGC 1 cut(s) 156
Tru1I TTAA 1 cut(s) 331
Tru9I TTAA 1 cut(s) 331
TseFI GTSAC 1 cut(s) 274
TseI GCWGC 2 cut(s) 105, 156
Tsp45I GTSAC 1 cut(s) 274
TspDTI ATGAA 3 cut(s) 135, 238, 253
Tth111I GACNNNGTC 1 cut(s) 34
ZraI GACGTC 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.