Rh4BG053600

Necessary for protein translocation in the endoplasmic reticulum

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0001066
Physical Location & Seq
Forward (+)
9595842 .. 9596099
258 bp
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UTR
Exon/CDS
Intron
Rh4BG053600.1

Sequence Viewer

Length: 252 bp
ATGGCTCCTCATGGTTCAGCTACTGCCACATCTAGAACGCGCAGCCGTCGTATCGGTGGAGGTGGCAATTCCAGCTCCGGAGGCATGGGCGGCAGCGGCGGTGGCTCTAGTACTGGAAACAACATACTCAGGTTCTACATCGACGACACTCCTGGCTTGAAGATCTCCCCCACCGTCATGCTCGTCATGAGCCTCTGCTTCATCGGCTTCATCACTGCCCTCCACGTCTTCGGCAAGCTCTACCTCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03060 ko04141 ko04145 ko05110 map03060 map04141 map04145 map05110
Pfam Domains
Protein Families
Sec61_beta

Protein Analysis

83

Amino Acids

8.5

Weight (kDa)

10.42

Isoelectric Point (pI)

62.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Sec61_beta PF03911 38 - 78 1.6e-14 Sec61beta family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 40
AccIII TCCGGA 1 cut(s) 77
AciI CCGC 3 cut(s) 90, 96, 99
AfaI GTAC 1 cut(s) 112
AgsI TTSAA 1 cut(s) 160
AjiI CACGTC 1 cut(s) 226
AjnI CCWGG 1 cut(s) 151
AluBI AGCT 3 cut(s) 20, 75, 238
AluI AGCT 3 cut(s) 20, 75, 238
AlwNI CAGNNNCTG 1 cut(s) 23
Aor13HI TCCGGA 1 cut(s) 77
ApeKI GCWGC 2 cut(s) 42, 93
AspLEI GCGC 1 cut(s) 42
BbsI GAAGAC 1 cut(s) 220
BbvI GCAGC 2 cut(s) 54, 105
BceAI ACGGC 1 cut(s) 30
BciT130I CCWGG 1 cut(s) 153
BfaI CTAG 2 cut(s) 33, 108
BglII AGATCT 1 cut(s) 162
BisI GCNGC 4 cut(s) 43, 91, 94, 97
BlsI GCNGC 4 cut(s) 44, 92, 95, 98
BmcAI AGTACT 1 cut(s) 112
Bme1390I CCNGG 1 cut(s) 153
BmgBI CACGTC 1 cut(s) 226
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 153
BpiI GAAGAC 1 cut(s) 220
BsaWI WCCGGW 1 cut(s) 77
Bse1I ACTGG 1 cut(s) 118
BseAI TCCGGA 1 cut(s) 77
BseBI CCWGG 1 cut(s) 153
BseMII CTCAG 1 cut(s) 142
BseNI ACTGG 1 cut(s) 118
BseXI GCAGC 2 cut(s) 54, 105
Bsh1236I CGCG 1 cut(s) 40
BsiSI CCGG 1 cut(s) 78
Bsp13I TCCGGA 1 cut(s) 77
Bsp143I GATC 1 cut(s) 162
BspACI CCGC 3 cut(s) 90, 96, 99
BspCNI CTCAG 1 cut(s) 141
BspEI TCCGGA 1 cut(s) 77
BspFNI CGCG 1 cut(s) 40
BspHI TCATGA 1 cut(s) 186
BspLI GGNNCC 1 cut(s) 6
BsrI ACTGG 1 cut(s) 118
BssMI GATC 1 cut(s) 162
Bst2UI CCWGG 1 cut(s) 153
Bst4CI ACNGT 1 cut(s) 175
BstC8I GCNNGC 1 cut(s) 236
BstDEI CTNAG 1 cut(s) 128
BstFNI CGCG 1 cut(s) 40
BstHHI GCGC 1 cut(s) 42
BstKTI GATC 1 cut(s) 165
BstMBI GATC 1 cut(s) 162
BstMWI GCNNNNNNNGC 6 cut(s) 72, 81, 90, 96, 102, 204
BstNI CCWGG 1 cut(s) 153
BstSCI CCNGG 1 cut(s) 151
BstUI CGCG 1 cut(s) 40
BstV1I GCAGC 2 cut(s) 54, 105
BstV2I GAAGAC 1 cut(s) 220
BstX2I RGATCY 1 cut(s) 162
BstYI RGATCY 1 cut(s) 162
BtrI CACGTC 1 cut(s) 226
BtsI GCAGTG 1 cut(s) 213
BtsIMutI CAGTG 2 cut(s) 213, 247
Cac8I GCNNGC 1 cut(s) 236
CaiI CAGNNNCTG 1 cut(s) 23
CciI TCATGA 1 cut(s) 186
CfoI GCGC 1 cut(s) 42
Csp6I GTAC 1 cut(s) 111
CviAII CATG 4 cut(s) 11, 85, 178, 187
CviJI RGCY 9 cut(s) 5, 20, 45, 75, 105, 156, 192, 207, 238
CviKI_1 RGCY 9 cut(s) 5, 20, 45, 75, 105, 156, 192, 207, 238
CviQI GTAC 1 cut(s) 111
DdeI CTNAG 1 cut(s) 128
DpnI GATC 1 cut(s) 164
DpnII GATC 1 cut(s) 162
EcoRII CCWGG 1 cut(s) 151
FaeI CATG 4 cut(s) 14, 88, 181, 190
FaiI YATR 5 cut(s) 12, 86, 125, 179, 188
FatI CATG 4 cut(s) 10, 84, 177, 186
Fnu4HI GCNGC 4 cut(s) 43, 91, 94, 97
Fsp4HI GCNGC 4 cut(s) 43, 91, 94, 97
FspBI CTAG 2 cut(s) 33, 108
GlaI GCGC 1 cut(s) 41
GluI GCNGC 4 cut(s) 43, 91, 94, 97
HapII CCGG 1 cut(s) 78
HhaI GCGC 1 cut(s) 42
Hin1II CATG 4 cut(s) 14, 88, 181, 190
Hin6I GCGC 1 cut(s) 40
HinP1I GCGC 1 cut(s) 40
HpaII CCGG 1 cut(s) 78
Hpy188III TCNNGA 3 cut(s) 33, 78, 187
Hpy99I CGWCG 2 cut(s) 51, 146
HpyCH4III ACNGT 1 cut(s) 175
HpyCH4IV ACGT 1 cut(s) 225
HpyF10VI GCNNNNNNNGC 6 cut(s) 72, 81, 90, 96, 102, 204
HpyF3I CTNAG 1 cut(s) 128
HpySE526I ACGT 1 cut(s) 225
Hsp92II CATG 4 cut(s) 14, 88, 181, 190
HspAI GCGC 1 cut(s) 40
Kpn2I TCCGGA 1 cut(s) 77
Kzo9I GATC 1 cut(s) 162
LmnI GCTCC 2 cut(s) 10, 80
LpnPI CCDG 6 cut(s) 85, 91, 99, 115, 138, 165
Lsp1109I GCAGC 2 cut(s) 54, 105
MaeI CTAG 2 cut(s) 33, 108
MaeII ACGT 1 cut(s) 225
MalI GATC 1 cut(s) 164
MboI GATC 1 cut(s) 162
MboII GAAGA 2 cut(s) 172, 220
MflI RGATCY 1 cut(s) 162
MluCI AATT 1 cut(s) 67
MnlI CCTC 5 cut(s) 18, 53, 74, 203, 230
MroI TCCGGA 1 cut(s) 77
MslI CAYNNNNRTG 1 cut(s) 176
MspA1I CMGCKG 1 cut(s) 96
MspI CCGG 1 cut(s) 78
MspR9I CCNGG 1 cut(s) 153
MvaI CCWGG 1 cut(s) 153
MvnI CGCG 1 cut(s) 40
MwoI GCNNNNNNNGC 6 cut(s) 72, 81, 90, 96, 102, 204
NdeII GATC 1 cut(s) 162
NlaIII CATG 4 cut(s) 14, 88, 181, 190
NlaIV GGNNCC 1 cut(s) 6
PagI TCATGA 1 cut(s) 186
PkrI GCNGC 4 cut(s) 44, 92, 95, 98
Psp6I CCWGG 1 cut(s) 151
PspGI CCWGG 1 cut(s) 151
PspN4I GGNNCC 1 cut(s) 6
PstNI CAGNNNCTG 1 cut(s) 23
PsuI RGATCY 1 cut(s) 162
RsaI GTAC 1 cut(s) 112
RsaNI GTAC 1 cut(s) 111
RseI CAYNNNNRTG 1 cut(s) 176
SatI GCNGC 4 cut(s) 43, 91, 94, 97
Sau3AI GATC 1 cut(s) 162
ScaI AGTACT 1 cut(s) 112
ScrFI CCNGG 1 cut(s) 153
SetI ASST 7 cut(s) 22, 64, 77, 134, 228, 240, 246
SmiMI CAYNNNNRTG 1 cut(s) 176
Sse9I AATT 1 cut(s) 67
SsiI CCGC 3 cut(s) 90, 96, 99
SspMI CTAG 2 cut(s) 33, 108
StyD4I CCNGG 1 cut(s) 151
TaaI ACNGT 1 cut(s) 175
TaiI ACGT 1 cut(s) 228
TaqI TCGA 1 cut(s) 141
TasI AATT 1 cut(s) 67
TatI WGTACW 1 cut(s) 110
TauI GCSGC 2 cut(s) 93, 99
TscAI CASTG 1 cut(s) 220
TseI GCWGC 2 cut(s) 42, 93
TspDTI ATGAA 2 cut(s) 190, 199
TspRI CASTG 1 cut(s) 220
XbaI TCTAGA 1 cut(s) 32
XspI CTAG 2 cut(s) 33, 108
ZrmI AGTACT 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.