Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0001204

Physical Location & Seq

Genomic Coordinates Forward (+)

25686193 .. 25692339

Gene Structure 6147 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G64220.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3153 bp

ATGGCGGATCGCGGATCTTTCGGATTTGCTCCTCGATTAGATATCAAGCAATTGCTGTCAGAAGCACAACACAGGTGGCTGAGACCTGCTGAGATTTGTGAAATTCTTCGGAATCATCAGAAGTTTCATATTGCGTCAGAGCCTCCCAACCGACCACCCAGTGGTTCACTCTTTCTCTTCGATAGGAAGGTGCTCAGATATTTCCGAAAAGACGGGCACAATTGGAGGAAGAAGAAAGATGGGAAAACAGTGAAAGAGGCTCACGAAAAGCTGAAGGTAGGAAGCATTGATGTGTTACATTGTTACTATGCACATGGAGAAGATAATGAGAACTTCCAGAGGCGGTGCTACTGGATGCTTGAACAGGATCTGATGCACATTGTTTTCGTTCACTACTTGGAGGTTAAGGGTAACCGAATGAGTACCAGTGGAACTAAAGAAAACCATTCAAATTCTCTGAGTGGCACGGGTTCTGTAAATGTTGATTCCACAGCAACCCGATCCAGCATATTGTCACCATTATGTGAAGATGCTGATTCTGGGGATAGTCGTCAAGCAAGTTCCAGTTTGCAACAAAATCCTGAACCTCAAACTGTTGTTCCTCAGATTATGCATCATCAGAATGCTAGCACAATCAATTCTTACAATACAACCTCTGTTTTGGGAAATCGAGATGGTTGGACGTCAGCTCATGGGAACAGGGTCAAAGGAAGTAACTCCCAAAGATCAGGTGATGTCCCAGCATGGGATGCTTCCTTTGAGAATTCATTGGCAAGATACCAGAATCTACCTTATAATGCTCCGTTGACTCAGACACAGCCTTCTACTTTTGGTTTGATCCCCATGGAAGGGAAAACAGAGAAAGGTTCCCTGTTAACGTCAGAGCATCTTAGAAATCCTCTTCAAAGTCAAGTAAATTGGCAGACTCCTGTTCAGGAAAGCGTGCCATTGCAAAAATGGCCCATGGATTCGCATTCTGGAATGACTGATGCTACTGATCTTGCCTTATTTGGGCAAGGAGCACATGAAAATTTTGGGACATTTTCCAGTCTTCTTGGTAGTCAAGATCAACAGTCTAGTAGTTTCCAAGCTCCTTTCACAAATAATGAAGCAGCATATATACCTAAATTAGGCCCCGAGGATCTTATATATGAAGCAAGTGCAAATCAAACACTACCTCTTAGAAAAGCATTGTTAAAAAAAGAGGATAGTTTGAAAAAGGTTGACAGTTTCTCTCGGTGGGTTAGTAAAGAACTTGGGGAGATGGAAGACTTGCAGATGCAATCTTCATCTGGAGGTATTGCATGGACTTCTGTTGAATGTGAAAACGCAGCAGCTGGGTCTTCCTTAAGTCCTTCTCTGTCCGAGGATCAGCGCTTCACTATGATCGACTTTTGGCCCAAATGGACTCAGACAGACTCAGAAGTTGAGGTAATGGTTATCGGGACATTTCTGTTGAGTCCTCAGGAAGTAACTAGCTATAGCTGGTCATGCATGTTTGGAGAAGTGGAGGTTCCGGCTGATATTTTAGTAGATGGTGTTCTTTGTTGTCATGCTCCGCCACATGAAGTCGGTCGAGTCCCGTTCTATATAACATGTTCTGACAGATTTTCTTGCAGTGAAGTACGAGAATTCGATTTCCTTCCTGGGTCTACCAGAAAGTTAAATGCTACTGACATTTATGGTGCCAATACAATTGAAACATCACTCCATTTGCGGTTTGAAAACTTGCTGGCTCTCAGATGTTCTGTTCAGGAACACCATATATTTGAAAATGTTGGGGAGAAACGAAGAAAAATCAGCAAAATCATGTTGCTAAAGGATGAAAAAGAGCCTCCTCTCCCCGGAACAATTGAGAAAGATCTAACAGAGCTGGAAGCAAAAGAGCGATTGATTAGGGAAGAGTTTGAAGATAAATTATATCTGTGGCTCATCCATAAGGTGACTGAAGAGGGCAAGGGTCCAAATATATTAGATGAAGATGGACAGGGTGTTCTACACCTAGCCGCAGCGCTGGGGTATGATTGGGCGATAAAGCCAATCTTAGCAGCAGGAGTTAGCATCAACTTCCGCGATGCAAATGGGTGGTCTGCTCTTCACTGGGCTGCATTCAGTGGCAGGGAGGATACTGTCGCTGTACTTGTCTCACTGGGTGCTGATGCTGGAGCATTGGCGGACCCATCTCCAGAACATCCTTTGGGGAAAACAGCTGCTGACTTGGCTTATGGAAACGGACACAGGGGAATATCGGGTTTCCTTGCAGAGTCATCCCTCACTAGCTACCTTGAAAAACTAACAGTGGACGCTAAGGAAAACAGCTCCGCTGACTCCTCTGGAGCAAAAGCTGTTCTGACAGTAGCTGAGCGAACAGCTACTCCTATGAGTTATGGTGATGTACCAGAAACACTTTCGATGAAGGATTCACTTACTGCTGTCTTAAATGCTACACAAGCAGCTGATCGTCTTCATCAAGTGTTCAGAATGCAGTCGTTCCAGCGGAAACAGCTCTCTGAACTTGGTGGCGACAACAAATTCGATATATCCGATGAGTTAGCTGTGTCTTTTGCGGCTGCAAAGACTAAGAAGTCAGGACACAGTAGTGGTGCTGTTCATGCTGCTGCGGTTCAAATCCAGAAAAAATACCGTGGTTGGAAGAAGAGAAAGGAGTTCCTTCTGATCCGACAAAGAATCGTCAAGATTCAGGCTCATGTGAGAGGACATCAAGTCCGAAAACAATACCGAGCCATTATTTGGTCCGTAGGATTGCTTGAGAAAATCATCTTGCGATGGAGGCGTAAAGGTAGTGGCTTACGCGGTTTCAAACGTGATACAATCAGCAAGCCAACAGAACCTGTATGTCCTGCCCCACAAGAAGATGATTATGATTTTCTCAAGGAAGGAAGGAAACAAACAGAGGAAAGGCTTCAAAAGGCTCTAACCCGAGTGAAGTCTATGGCTCAATATCCAGAGGCACGGGCTCAATACCGTAGACTATTGACTGTCGTTGAAGGCTTCCGTGAGAACGAGGCTTCTTCTAGCTCGGCATTGAAAAACAACACAGAGGAAGCTGCAAATTACAACGAAGAAGACGATTTAATTGACATTGATTCTCTTTTGGATGACGACACTTTCATGTCTCTTGCATTTGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000981 GO:0000982 GO:0001077 GO:0001101 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010044 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050826 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071241 GO:0071248 GO:0071275 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901700 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 CG-1 IQ TIG

Protein Analysis

1050

Amino Acids

117.26

Weight (kDa)

6.0

Isoelectric Point (pI)

45.43

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CG-1	PF03859	21 - 134	2.1e-52	CG-1 domain
Ank_2	PF12796	649 - 721	1.7e-09	Ankyrin repeats (3 copies)
Ank_2	PF12796	666 - 755	1.8e-08	Ankyrin repeats (3 copies)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0001204)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G09410 AT5G09410 AT5G09410 AT5G09410 AT5G64220 AT5G64220
fragaria_vesca	FvH4_5g27670 FvH4_5g27670 FvH4_5g27670 FvH4_5g27670
malus_domestica	MD05G1044600.v1.1 MD10G1051700.v1.1 MD12G1040100.v1.1 MD13G1144300.v1.1 MD16G1084000.v1.1 MD16G1139000.v1.1 MD16G1139200.v1.1
prunus_persica	Prupe.1G108700_v2.0.a1 Prupe.1G108700_v2.0.a1 Prupe.1G108700_v2.0.a1 Prupe.1G108700_v2.0.a1 Prupe.1G108700_v2.0.a1 Prupe.8G060300_v2.0.a1
pyrus_communis	pycom01g00940 pycom05g03720 pycom10g03650 pycom12g03420 pycom13g12490 pycom16g12350 pycom16g12360
rosa_chinensis	RchiOBHm_Chr2g0113081 RchiOBHm_Chr7g0219321
rosa_laevigata	RLG00000002297 RLG00000022767
rosa_multiflora	Rmu_sc0029995.1_g000002
rosa_roxburghii	Rroxscaffold_2G00129370 Rroxscaffold_3G00240090
rosa_rugosa	Rorug01G0154600.1 Rorug07G0184300
rosa_samantha	Rh2AG238800 Rh2AG238900 Rh2AG324900 Rh2BG252600 Rh2CG242800 Rh2CG242900 Rh2DG246800 Rh4BG352900 Rh5AG518400 Rh7AG327200 Rh7BG317200 Rh7CG344500 Rh7DG209600 Rh7DG322800
rosa_wichuraiana	Rw0G004510 Rw2G018730 Rw7G027570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	795
AasI	GACNNNNNNGTC	1 cut(s)	2584
AatII	GACGTC	1 cut(s)	686
Acc36I	ACCTGC	1 cut(s)	94
AccB1I	GGYRCC	1 cut(s)	1685
AccB7I	CCANNNNNTGG	2 cut(s)	161, 2751
AccI	GTMKAC	2 cut(s)	1652, 2989
AccII	CGCG	3 cut(s)	12, 2073, 2814
AclWI	GGATC	8 cut(s)	15, 22, 375, 495, 832, 1149, 1377, 2671
AcsI	RAATTY	6 cut(s)	102, 451, 763, 1030, 1631, 2531
AcuI	CTGAAG	2 cut(s)	293, 1968
AcyI	GRCGYC	1 cut(s)	683
AdeI	CACNNNGTG	2 cut(s)	161, 2153
AfaI	GTAC	4 cut(s)	424, 1626, 2139, 2397
AfeI	AGCGCT	2 cut(s)	1376, 2013
AfiI	CCNNNNNNNGG	8 cut(s)	161, 745, 848, 849, 1011, 1130, 1844, 2751
AflII	CTTAAG	1 cut(s)	1348
AflIII	ACRYGT	1 cut(s)	1595
AhdI	GACNNNNNGTC	1 cut(s)	2723
AjnI	CCWGG	1 cut(s)	1645
AleI	CACNNNNGTG	1 cut(s)	2598
AloI	GAACNNNNNNTCC	4 cut(s)	1977, 2009, 2359, 2391
Alw21I	GWGCWC	2 cut(s)	195, 1024
Alw26I	GTCTC	3 cut(s)	76, 2149, 3141
AlwI	GGATC	8 cut(s)	15, 22, 375, 495, 832, 1149, 1377, 2671
AlwNI	CAGNNNCTG	5 cut(s)	370, 1337, 2213, 2360, 2852
Ama87I	CYCGRG	2 cut(s)	1136, 2940
Aor51HI	AGCGCT	2 cut(s)	1376, 2013
AoxI	GGCC	3 cut(s)	959, 1132, 1397
ApoI	RAATTY	6 cut(s)	102, 451, 763, 1030, 1631, 2531
ArsI	GACNNNNNNTTYG	4 cut(s)	2072, 2104, 2516, 2548
Asp700I	GAANNNNTTC	2 cut(s)	105, 2922
AspLEI	GCGC	2 cut(s)	1377, 2014
AspS9I	GGNCC	6 cut(s)	960, 1133, 1398, 1961, 2176, 2754
AsuC2I	CCSGG	1 cut(s)	1845
AsuHPI	GGTGA	4 cut(s)	507, 743, 1954, 2402
AsuNHI	GCTAGC	1 cut(s)	626
AvaI	CYCGRG	2 cut(s)	1136, 2940
AvaII	GGWCC	3 cut(s)	1961, 2176, 2754
AxyI	CCTNAGG	1 cut(s)	1464
BaeGI	GKGCMC	1 cut(s)	219
BanI	GGYRCC	1 cut(s)	1685
BanII	GRGCYC	1 cut(s)	2980
BbsI	GAAGAC	5 cut(s)	1043, 1275, 1335, 2456, 3093
Bbv12I	GWGCWC	2 cut(s)	195, 1024
BccI	CCATC	7 cut(s)	233, 668, 1258, 1529, 1976, 2188, 2781
BciT130I	CCWGG	1 cut(s)	1647
BciVI	GTATCC	1 cut(s)	2119
BcnI	CCSGG	1 cut(s)	1845
BcoDI	GTCTC	3 cut(s)	76, 2149, 3141
BfaI	CTAG	6 cut(s)	627, 1077, 1476, 2003, 2277, 3036
BfmI	CTRYAG	1 cut(s)	1480
BfoI	RGCGCY	2 cut(s)	1378, 2015
BfrI	CTTAAG	1 cut(s)	1348
BfuAI	ACCTGC	1 cut(s)	94
BfuI	GTATCC	1 cut(s)	2119
BglII	AGATCT	1 cut(s)	1861
BlpI	GCTNAGC	1 cut(s)	2361
Bme1390I	CCNGG	2 cut(s)	1647, 1845
Bme18I	GGWCC	3 cut(s)	1961, 2176, 2754
BmeRI	GACNNNNNGTC	1 cut(s)	2723
BmeT110I	CYCGRG	2 cut(s)	1136, 2940
BmgT120I	GGNCC	6 cut(s)	960, 1133, 1398, 1961, 2176, 2754
BmiI	GGNNCC	6 cut(s)	868, 1135, 1515, 1687, 1962, 2178
BmrFI	CCNGG	2 cut(s)	1647, 1845
BmrI	ACTGGG	3 cut(s)	153, 2110, 2159
BmtI	GCTAGC	1 cut(s)	630
BmuI	ACTGGG	3 cut(s)	153, 2110, 2159
BpiI	GAAGAC	5 cut(s)	1043, 1275, 1335, 2456, 3093
BpmI	CTGGAG	4 cut(s)	1314, 2169, 2184, 2355
Bpu10I	CCTNAGC	1 cut(s)	2307
Bpu1102I	GCTNAGC	1 cut(s)	2361
BpuEI	CTTGAG	2 cut(s)	2789, 2876
BpuMI	CCSGG	1 cut(s)	1845
BsaHI	GRCGYC	1 cut(s)	683
BsaI	GGTCTC	1 cut(s)	76
BsaJI	CCNNGG	7 cut(s)	843, 963, 1137, 1365, 1646, 1843, 2644
BsaXI	ACNNNNNCTCC	2 cut(s)	2359, 2389
Bsc4I	CCNNNNNNNGG	8 cut(s)	161, 745, 848, 849, 1011, 1130, 1844, 2751
Bse1I	ACTGG	7 cut(s)	159, 356, 426, 564, 1047, 2105, 2154
Bse21I	CCTNAGG	1 cut(s)	1464
Bse3DI	GCAATG	1 cut(s)	947
BseBI	CCWGG	1 cut(s)	1647
BseDI	CCNNGG	7 cut(s)	843, 963, 1137, 1365, 1646, 1843, 2644
BseGI	GGATG	7 cut(s)	360, 754, 1828, 1932, 2191, 2267, 3124
BseLI	CCNNNNNNNGG	8 cut(s)	161, 745, 848, 849, 1011, 1130, 1844, 2751
BseMI	GCAATG	1 cut(s)	947
BseNI	ACTGG	7 cut(s)	159, 356, 426, 564, 1047, 2105, 2154
BseRI	GAGGAG	3 cut(s)	21, 1827, 2320
BseSI	GKGCMC	1 cut(s)	219
BseYI	CCCAGC	3 cut(s)	739, 1337, 2014
Bsh1236I	CGCG	3 cut(s)	12, 2073, 2814
Bsh1285I	CGRYCG	1 cut(s)	1576
BshFI	GGCC	3 cut(s)	961, 1134, 1399
BshNI	GGYRCC	1 cut(s)	1685
BsiEI	CGRYCG	1 cut(s)	1576
BsiHKAI	GWGCWC	2 cut(s)	195, 1024
BsiHKCI	CYCGRG	2 cut(s)	1136, 2940
BsiSI	CCGG	2 cut(s)	1517, 1845
BslFI	GGGAC	4 cut(s)	722, 1051, 1459, 1565
BslI	CCNNNNNNNGG	8 cut(s)	161, 745, 848, 849, 1011, 1130, 1844, 2751
BsmAI	GTCTC	3 cut(s)	76, 2149, 3141
BsmFI	GGGAC	4 cut(s)	722, 1051, 1459, 1565
BsmI	GAATGC	4 cut(s)	628, 973, 2108, 2487
BsnI	GGCC	3 cut(s)	961, 1134, 1399
Bso31I	GGTCTC	1 cut(s)	76
BsoBI	CYCGRG	2 cut(s)	1136, 2940
Bsp1286I	GDGCHC	4 cut(s)	195, 219, 1024, 2980
Bsp1720I	GCTNAGC	1 cut(s)	2361
Bsp19I	CCATGG	2 cut(s)	843, 963
BspANI	GGCC	3 cut(s)	961, 1134, 1399
BspFNI	CGCG	3 cut(s)	12, 2073, 2814
BspLI	GGNNCC	6 cut(s)	868, 1135, 1515, 1687, 1962, 2178
BspMI	ACCTGC	1 cut(s)	94
BspOI	GCTAGC	1 cut(s)	630
BspPI	GGATC	8 cut(s)	15, 22, 375, 495, 832, 1149, 1377, 2671
BspQI	GCTCTTC	1 cut(s)	2100
BspT107I	GGYRCC	1 cut(s)	1685
BspTI	CTTAAG	1 cut(s)	1348
BspTNI	GGTCTC	1 cut(s)	76
BsrDI	GCAATG	1 cut(s)	947
BsrI	ACTGG	7 cut(s)	159, 356, 426, 564, 1047, 2105, 2154
BssECI	CCNNGG	7 cut(s)	843, 963, 1137, 1365, 1646, 1843, 2644
BssNI	GRCGYC	1 cut(s)	683
BssT1I	CCWWGG	2 cut(s)	843, 963
Bst2UI	CCWGG	1 cut(s)	1647
Bst6I	CTCTTC	6 cut(s)	182, 906, 1896, 1944, 2100, 2651
BstACI	GRCGYC	1 cut(s)	683
BstAFI	CTTAAG	1 cut(s)	1348
BstAPI	GCANNNNNTGC	1 cut(s)	749
BstC8I	GCNNGC	4 cut(s)	628, 944, 1734, 2840
BstDSI	CCRYGG	3 cut(s)	843, 963, 2644
BstEII	GGTNACC	1 cut(s)	410
BstENI	CCTNNNNNAGG	1 cut(s)	1128
BstF5I	GGATG	7 cut(s)	360, 754, 1828, 1932, 2191, 2267, 3124
BstFNI	CGCG	3 cut(s)	12, 2073, 2814
BstH2I	RGCGCY	2 cut(s)	1378, 2015
BstHHI	GCGC	2 cut(s)	1377, 2014
BstMAI	GTCTC	3 cut(s)	76, 2149, 3141
BstMCI	CGRYCG	1 cut(s)	1576
BstMWI	GCNNNNNNNGC	8 cut(s)	749, 958, 1491, 2219, 2450, 2503, 2612, 2791
BstNI	CCWGG	1 cut(s)	1647
BstNSI	RCATGY	2 cut(s)	1498, 1599
BstPI	GGTNACC	1 cut(s)	410
BstSCI	CCNGG	2 cut(s)	1645, 1843
BstSFI	CTRYAG	1 cut(s)	1480
BstSLI	GKGCMC	1 cut(s)	219
BstUI	CGCG	3 cut(s)	12, 2073, 2814
BstV2I	GAAGAC	5 cut(s)	1043, 1275, 1335, 2456, 3093
BstX2I	RGATCY	4 cut(s)	14, 367, 1141, 1861
BstYI	RGATCY	4 cut(s)	14, 367, 1141, 1861
Bsu36I	CCTNAGG	1 cut(s)	1464
BsuI	GTATCC	1 cut(s)	2119
BsuRI	GGCC	3 cut(s)	961, 1134, 1399
BtgI	CCRYGG	3 cut(s)	843, 963, 2644
BtgZI	GCGATG	2 cut(s)	2088, 2800
BtsCI	GGATG	7 cut(s)	360, 754, 1828, 1932, 2191, 2267, 3124
BtsI	GCAGTG	1 cut(s)	1624
BtsIMutI	CAGTG	8 cut(s)	166, 255, 433, 1624, 2098, 2119, 2147, 2304
BveI	ACCTGC	1 cut(s)	94
Cac8I	GCNNGC	4 cut(s)	628, 944, 1734, 2840
CaiI	CAGNNNCTG	5 cut(s)	370, 1337, 2213, 2360, 2852
CfoI	GCGC	2 cut(s)	1377, 2014
Cfr13I	GGNCC	6 cut(s)	960, 1133, 1398, 1961, 2176, 2754
CseI	GACGC	2 cut(s)	123, 2312
Csp6I	GTAC	4 cut(s)	423, 1625, 2138, 2396
CspCI	CAANNNNNGTGG	2 cut(s)	56, 91
CviQI	GTAC	4 cut(s)	423, 1625, 2138, 2396
DraIII	CACNNNGTG	2 cut(s)	161, 2153
DrdI	GACNNNNNNGTC	1 cut(s)	2584
DriI	GACNNNNNGTC	1 cut(s)	2723
DseDI	GACNNNNNNGTC	1 cut(s)	2584
Eam1104I	CTCTTC	6 cut(s)	182, 906, 1896, 1944, 2100, 2651
Eam1105I	GACNNNNNGTC	1 cut(s)	2723
EarI	CTCTTC	6 cut(s)	182, 906, 1896, 1944, 2100, 2651
EciI	GGCGGA	3 cut(s)	20, 1548, 2189
Eco130I	CCWWGG	2 cut(s)	843, 963
Eco24I	GRGCYC	1 cut(s)	2980
Eco31I	GGTCTC	1 cut(s)	76
Eco32I	GATATC	1 cut(s)	43
Eco47I	GGWCC	3 cut(s)	1961, 2176, 2754
Eco47III	AGCGCT	2 cut(s)	1376, 2013
Eco57I	CTGAAG	2 cut(s)	293, 1968
Eco81I	CCTNAGG	1 cut(s)	1464
Eco88I	CYCGRG	2 cut(s)	1136, 2940
Eco91I	GGTNACC	1 cut(s)	410
EcoNI	CCTNNNNNAGG	1 cut(s)	1128
EcoO109I	RGGNCCY	1 cut(s)	1133
EcoO65I	GGTNACC	1 cut(s)	410
EcoRI	GAATTC	2 cut(s)	763, 1631
EcoRII	CCWGG	1 cut(s)	1645
EcoRV	GATATC	1 cut(s)	43
EcoT14I	CCWWGG	2 cut(s)	843, 963
EcoT22I	ATGCAT	2 cut(s)	615, 1496
EcoT38I	GRGCYC	1 cut(s)	2980
ErhI	CCWWGG	2 cut(s)	843, 963
FalI	AAGNNNNNCTT	2 cut(s)	2027, 2059
FaqI	GGGAC	4 cut(s)	722, 1051, 1459, 1565
FblI	GTMKAC	2 cut(s)	1652, 2989
FokI	GGATG	7 cut(s)	367, 761, 1835, 1919, 2178, 2254, 3131
FriOI	GRGCYC	1 cut(s)	2980
FspBI	CTAG	6 cut(s)	627, 1077, 1476, 2003, 2277, 3036
GlaI	GCGC	2 cut(s)	1376, 2013
GsaI	CCCAGC	3 cut(s)	743, 1341, 2018
GsuI	CTGGAG	4 cut(s)	1314, 2169, 2184, 2355
HaeII	RGCGCY	2 cut(s)	1378, 2015
HaeIII	GGCC	3 cut(s)	961, 1134, 1399
HapII	CCGG	2 cut(s)	1517, 1845
HgaI	GACGC	2 cut(s)	123, 2312
HhaI	GCGC	2 cut(s)	1377, 2014
Hin1I	GRCGYC	1 cut(s)	683
Hin6I	GCGC	2 cut(s)	1375, 2012
HinP1I	GCGC	2 cut(s)	1375, 2012
HincII	GTYRAC	3 cut(s)	807, 876, 1225
HindII	GTYRAC	3 cut(s)	807, 876, 1225
HpaI	GTTAAC	1 cut(s)	876
HpaII	CCGG	2 cut(s)	1517, 1845
HphI	GGTGA	4 cut(s)	507, 743, 1954, 2402
Hpy166II	GTNNAC	8 cut(s)	167, 391, 807, 876, 1225, 1653, 2302, 2990
Hpy8I	GTNNAC	8 cut(s)	167, 391, 807, 876, 1225, 1653, 2302, 2990
HpyCH4IV	ACGT	3 cut(s)	683, 878, 2824
HpyF10VI	GCNNNNNNNGC	8 cut(s)	749, 958, 1491, 2219, 2450, 2503, 2612, 2791
HpySE526I	ACGT	3 cut(s)	683, 878, 2824
Hsp92I	GRCGYC	1 cut(s)	683
HspAI	GCGC	2 cut(s)	1375, 2012
KspAI	GTTAAC	1 cut(s)	876
LguI	GCTCTTC	1 cut(s)	2100
LmnI	GCTCC	8 cut(s)	34, 805, 1019, 1096, 1561, 2165, 2324, 2336
MaeI	CTAG	6 cut(s)	627, 1077, 1476, 2003, 2277, 3036
MaeII	ACGT	3 cut(s)	683, 878, 2824
MaeIII	GTNAC	7 cut(s)	294, 302, 410, 513, 713, 1471, 1942
MfeI	CAATTG	4 cut(s)	50, 220, 1695, 1851
MflI	RGATCY	4 cut(s)	14, 367, 1141, 1861
MhlI	GDGCHC	4 cut(s)	195, 219, 1024, 2980
MlyI	GAGTC	8 cut(s)	802, 919, 1402, 1412, 1468, 1587, 2273, 2321
MmeI	TCCRAC	3 cut(s)	659, 2630, 2704
Mph1103I	ATGCAT	2 cut(s)	615, 1496
MroXI	GAANNNNTTC	2 cut(s)	105, 2922
MseI	TTAA	7 cut(s)	405, 875, 1196, 1349, 1664, 2438, 3095
MslI	CAYNNNNRTG	6 cut(s)	290, 520, 621, 2598, 3131, 3148
MspA1I	CMGCKG	5 cut(s)	1337, 2210, 2324, 2456, 2497
MspCI	CTTAAG	1 cut(s)	1348
MspI	CCGG	2 cut(s)	1517, 1845
MspR9I	CCNGG	2 cut(s)	1647, 1845
MunI	CAATTG	4 cut(s)	50, 220, 1695, 1851
Mva1269I	GAATGC	4 cut(s)	628, 973, 2108, 2487
MvaI	CCWGG	1 cut(s)	1647
MvnI	CGCG	3 cut(s)	12, 2073, 2814
MwoI	GCNNNNNNNGC	8 cut(s)	749, 958, 1491, 2219, 2450, 2503, 2612, 2791
NciI	CCSGG	1 cut(s)	1845
NcoI	CCATGG	2 cut(s)	843, 963
NheI	GCTAGC	1 cut(s)	626
NlaIV	GGNNCC	6 cut(s)	868, 1135, 1515, 1687, 1962, 2178
NmeAIII	GCCGAG	1 cut(s)	3020
NmuCI	GTSAC	2 cut(s)	513, 1942
NsiI	ATGCAT	2 cut(s)	615, 1496
NspI	RCATGY	2 cut(s)	1498, 1599
OliI	CACNNNNGTG	1 cut(s)	2598
PciI	ACATGT	1 cut(s)	1595
PciSI	GCTCTTC	1 cut(s)	2100
PcsI	WCGNNNNNNNCGW	2 cut(s)	2541, 3087
PctI	GAATGC	4 cut(s)	628, 973, 2108, 2487
PdmI	GAANNNNTTC	2 cut(s)	105, 2922
PfeI	GAWTC	9 cut(s)	112, 485, 536, 784, 968, 2420, 2688, 2698, 3107
PflMI	CCANNNNNTGG	2 cut(s)	161, 2751
PleI	GAGTC	8 cut(s)	802, 919, 1402, 1412, 1467, 1586, 2272, 2321
PpsI	GAGTC	8 cut(s)	802, 919, 1402, 1412, 1467, 1586, 2272, 2321
PscI	ACATGT	1 cut(s)	1595
PsiI	TTATAA	1 cut(s)	795
Psp6I	CCWGG	1 cut(s)	1645
PspEI	GGTNACC	1 cut(s)	410
PspFI	CCCAGC	3 cut(s)	739, 1337, 2014
PspGI	CCWGG	1 cut(s)	1645
PspN4I	GGNNCC	6 cut(s)	868, 1135, 1515, 1687, 1962, 2178
PspPI	GGNCC	6 cut(s)	960, 1133, 1398, 1961, 2176, 2754
PsrI	GAACNNNNNNTAC	2 cut(s)	1524, 1556
PstNI	CAGNNNCTG	5 cut(s)	370, 1337, 2213, 2360, 2852
PsuI	RGATCY	4 cut(s)	14, 367, 1141, 1861
PvuII	CAGCTG	3 cut(s)	1337, 2210, 2456
RsaI	GTAC	4 cut(s)	424, 1626, 2139, 2397
RsaNI	GTAC	4 cut(s)	423, 1625, 2138, 2396
RseI	CAYNNNNRTG	6 cut(s)	290, 520, 621, 2598, 3131, 3148
SapI	GCTCTTC	1 cut(s)	2100
SaqAI	TTAA	7 cut(s)	405, 875, 1196, 1349, 1664, 2438, 3095
Sau96I	GGNCC	6 cut(s)	960, 1133, 1398, 1961, 2176, 2754
SchI	GAGTC	8 cut(s)	802, 919, 1402, 1412, 1468, 1587, 2273, 2321
ScrFI	CCNGG	2 cut(s)	1647, 1845
SduI	GDGCHC	4 cut(s)	195, 219, 1024, 2980
SfcI	CTRYAG	1 cut(s)	1480
SinI	GGWCC	3 cut(s)	1961, 2176, 2754
SmiMI	CAYNNNNRTG	6 cut(s)	290, 520, 621, 2598, 3131, 3148
SmlI	CTYRAG	3 cut(s)	1348, 2768, 2891
SmoI	CTYRAG	3 cut(s)	1348, 2768, 2891
SspMI	CTAG	6 cut(s)	627, 1077, 1476, 2003, 2277, 3036
StyD4I	CCNGG	2 cut(s)	1645, 1843
StyI	CCWWGG	2 cut(s)	843, 963
TaiI	ACGT	3 cut(s)	686, 881, 2827
TaqI	TCGA	8 cut(s)	34, 180, 670, 1389, 1576, 1635, 2411, 2535
TaqII	GACCGA	1 cut(s)	1562
TatI	WGTACW	1 cut(s)	2137
TauI	GCSGC	2 cut(s)	2009, 2570
TfiI	GAWTC	9 cut(s)	112, 485, 536, 784, 968, 2420, 2688, 2698, 3107
Tru1I	TTAA	7 cut(s)	405, 875, 1196, 1349, 1664, 2438, 3095
Tru9I	TTAA	7 cut(s)	405, 875, 1196, 1349, 1664, 2438, 3095
TscAI	CASTG	8 cut(s)	166, 255, 433, 1624, 2105, 2119, 2154, 2304
TseFI	GTSAC	2 cut(s)	513, 1942
Tsp45I	GTSAC	2 cut(s)	513, 1942
TspGWI	ACGGA	4 cut(s)	792, 2247, 2746, 3005
TspRI	CASTG	8 cut(s)	166, 255, 433, 1624, 2105, 2119, 2154, 2304
Van91I	CCANNNNNTGG	2 cut(s)	161, 2751
Vha464I	CTTAAG	1 cut(s)	1348
VpaK11BI	GGWCC	3 cut(s)	1961, 2176, 2754
XagI	CCTNNNNNAGG	1 cut(s)	1128
XapI	RAATTY	6 cut(s)	102, 451, 763, 1030, 1631, 2531
XceI	RCATGY	2 cut(s)	1498, 1599
XcmI	CCANNNNNNNNNTGG	1 cut(s)	954
XmiI	GTMKAC	2 cut(s)	1652, 2989
XmnI	GAANNNNTTC	2 cut(s)	105, 2922
XspI	CTAG	6 cut(s)	627, 1077, 1476, 2003, 2277, 3036
ZraI	GACGTC	1 cut(s)	684
Zsp2I	ATGCAT	2 cut(s)	615, 1496

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.