Rh4BG352900

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0001204
Physical Location & Seq
Reverse (-)
51328164 .. 51328463
300 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG352900.1

Sequence Viewer

Length: 300 bp
ATGGAGGAGGCTGACCGGGCCCGGGTGGAGGTCCCTACAGAGCCCTTCCAGGTTGTCACCGCCGAGTCCGTGCTGGTTCCACCTGTGCTCCACCCCCTCCACGTCACCCGCGAAAACCAACAGAAACGGCGGCAGCGACCCCAGCTCGTATATACGGTGCCGTTTCTGGACGCTCATCCAGTGCTCCAGCTTCTCCGTGTACTTGCCCTCCCTCCACTTCCACAGATCGATCACCATAACCCCCGTGTTGAAATAGCACGCGCTCCGCCCCGAGAACTCCGCCGCCAGCGCCGCGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

99

Amino Acids

11.56

Weight (kDa)

10.97

Isoelectric Point (pI)

78.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 157
AccII CGCG 3 cut(s) 111, 261, 294
AciI CCGC 7 cut(s) 60, 109, 130, 266, 280, 283, 292
AfaI GTAC 1 cut(s) 201
AfiI CCNNNNNNNGG 2 cut(s) 22, 28
AgsI TTSAA 1 cut(s) 251
AjiI CACGTC 1 cut(s) 103
AjnI CCWGG 1 cut(s) 48
AluBI AGCT 2 cut(s) 145, 190
AluI AGCT 2 cut(s) 145, 190
Alw21I GWGCWC 2 cut(s) 90, 186
Ama87I CYCGRG 2 cut(s) 21, 270
AoxI GGCC 1 cut(s) 18
ApaI GGGCCC 1 cut(s) 22
ApeKI GCWGC 1 cut(s) 133
AspLEI GCGC 2 cut(s) 263, 291
AspS9I GGNCC 3 cut(s) 18, 19, 31
AsuC2I CCSGG 3 cut(s) 17, 22, 23
AsuHPI GGTGA 3 cut(s) 49, 97, 224
AvaI CYCGRG 2 cut(s) 21, 270
AvaII GGWCC 1 cut(s) 31
BaeGI GKGCMC 1 cut(s) 22
BanI GGYRCC 1 cut(s) 157
BanII GRGCYC 2 cut(s) 22, 45
Bbv12I GWGCWC 2 cut(s) 90, 186
BbvI GCAGC 1 cut(s) 145
BceAI ACGGC 2 cut(s) 143, 145
BciT130I CCWGG 1 cut(s) 50
BcnI CCSGG 3 cut(s) 17, 22, 23
BfmI CTRYAG 1 cut(s) 36
BfoI RGCGCY 1 cut(s) 292
BisI GCNGC 4 cut(s) 131, 134, 283, 292
BlsI GCNGC 4 cut(s) 132, 135, 284, 293
Bme1390I CCNGG 4 cut(s) 17, 22, 23, 50
Bme18I GGWCC 1 cut(s) 31
BmeT110I CYCGRG 2 cut(s) 21, 270
BmgBI CACGTC 1 cut(s) 103
BmgT120I GGNCC 3 cut(s) 18, 19, 31
BmiI GGNNCC 4 cut(s) 20, 33, 78, 159
BmrFI CCNGG 4 cut(s) 17, 22, 23, 50
BpmI CTGGAG 1 cut(s) 170
BpuMI CCSGG 3 cut(s) 17, 22, 23
Bsa29I ATCGAT 1 cut(s) 228
BsaJI CCNNGG 1 cut(s) 21
BsaXI ACNNNNNCTCC 4 cut(s) 72, 102, 192, 222
Bsc4I CCNNNNNNNGG 2 cut(s) 22, 28
Bse1I ACTGG 1 cut(s) 179
BseBI CCWGG 1 cut(s) 50
BseCI ATCGAT 1 cut(s) 228
BseDI CCNNGG 1 cut(s) 21
BseGI GGATG 1 cut(s) 175
BseLI CCNNNNNNNGG 2 cut(s) 22, 28
BseNI ACTGG 1 cut(s) 179
BseRI GAGGAG 1 cut(s) 20
BseSI GKGCMC 1 cut(s) 22
BseXI GCAGC 1 cut(s) 145
BseYI CCCAGC 1 cut(s) 141
Bsh1236I CGCG 3 cut(s) 111, 261, 294
BshFI GGCC 1 cut(s) 20
BshNI GGYRCC 1 cut(s) 157
BshVI ATCGAT 1 cut(s) 228
BsiHKAI GWGCWC 2 cut(s) 90, 186
BsiHKCI CYCGRG 2 cut(s) 21, 270
BsiSI CCGG 2 cut(s) 16, 22
BslFI GGGAC 1 cut(s) 17
BslI CCNNNNNNNGG 2 cut(s) 22, 28
BsmFI GGGAC 1 cut(s) 17
BsnI GGCC 1 cut(s) 20
BsoBI CYCGRG 2 cut(s) 21, 270
Bsp120I GGGCCC 1 cut(s) 18
Bsp1286I GDGCHC 4 cut(s) 22, 45, 90, 186
Bsp143I GATC 2 cut(s) 225, 229
BspACI CCGC 7 cut(s) 60, 109, 130, 266, 280, 283, 292
BspANI GGCC 1 cut(s) 20
BspDI ATCGAT 1 cut(s) 228
BspFNI CGCG 3 cut(s) 111, 261, 294
BspLI GGNNCC 4 cut(s) 20, 33, 78, 159
BspT107I GGYRCC 1 cut(s) 157
BsrI ACTGG 1 cut(s) 179
BssECI CCNNGG 1 cut(s) 21
BssMI GATC 2 cut(s) 225, 229
Bst2UI CCWGG 1 cut(s) 50
Bst4CI ACNGT 1 cut(s) 157
BstC8I GCNNGC 2 cut(s) 259, 287
BstF5I GGATG 1 cut(s) 175
BstFNI CGCG 3 cut(s) 111, 261, 294
BstH2I RGCGCY 1 cut(s) 292
BstHHI GCGC 2 cut(s) 263, 291
BstKTI GATC 2 cut(s) 228, 232
BstMBI GATC 2 cut(s) 225, 229
BstMWI GCNNNNNNNGC 4 cut(s) 17, 142, 288, 291
BstNI CCWGG 1 cut(s) 50
BstSCI CCNGG 4 cut(s) 15, 20, 21, 48
BstSFI CTRYAG 1 cut(s) 36
BstSLI GKGCMC 1 cut(s) 22
BstUI CGCG 3 cut(s) 111, 261, 294
BstV1I GCAGC 1 cut(s) 145
Bsu15I ATCGAT 1 cut(s) 228
BsuRI GGCC 1 cut(s) 20
BsuTUI ATCGAT 1 cut(s) 228
BtrI CACGTC 1 cut(s) 103
BtsCI GGATG 1 cut(s) 175
BtsIMutI CAGTG 1 cut(s) 186
Cac8I GCNNGC 2 cut(s) 259, 287
CfoI GCGC 2 cut(s) 263, 291
Cfr13I GGNCC 3 cut(s) 18, 19, 31
Cfr9I CCCGGG 1 cut(s) 21
ClaI ATCGAT 1 cut(s) 228
CseI GACGC 1 cut(s) 179
Csp6I GTAC 1 cut(s) 200
CviJI RGCY 5 cut(s) 11, 20, 43, 145, 190
CviKI_1 RGCY 5 cut(s) 11, 20, 43, 145, 190
CviQI GTAC 1 cut(s) 200
DpnI GATC 2 cut(s) 227, 231
DpnII GATC 2 cut(s) 225, 229
EciI GGCGGA 2 cut(s) 255, 269
Eco24I GRGCYC 2 cut(s) 22, 45
Eco47I GGWCC 1 cut(s) 31
Eco88I CYCGRG 2 cut(s) 21, 270
EcoO109I RGGNCCY 1 cut(s) 31
EcoRII CCWGG 1 cut(s) 48
EcoT38I GRGCYC 2 cut(s) 22, 45
FaiI YATR 3 cut(s) 151, 153, 237
FaqI GGGAC 1 cut(s) 17
FauI CCCGC 1 cut(s) 116
Fnu4HI GCNGC 4 cut(s) 131, 134, 283, 292
FokI GGATG 1 cut(s) 162
FriOI GRGCYC 2 cut(s) 22, 45
Fsp4HI GCNGC 4 cut(s) 131, 134, 283, 292
GlaI GCGC 2 cut(s) 262, 290
GluI GCNGC 4 cut(s) 131, 134, 283, 292
GsaI CCCAGC 1 cut(s) 145
GsuI CTGGAG 1 cut(s) 170
HaeII RGCGCY 1 cut(s) 292
HaeIII GGCC 1 cut(s) 20
HapII CCGG 2 cut(s) 16, 22
HgaI GACGC 1 cut(s) 179
HhaI GCGC 2 cut(s) 263, 291
Hin6I GCGC 2 cut(s) 261, 289
HinP1I GCGC 2 cut(s) 261, 289
HinfI GANTC 1 cut(s) 65
HpaII CCGG 2 cut(s) 16, 22
HphI GGTGA 3 cut(s) 49, 97, 224
Hpy166II GTNNAC 1 cut(s) 200
Hpy188III TCNNGA 1 cut(s) 167
Hpy8I GTNNAC 1 cut(s) 200
HpyAV CCTTC 1 cut(s) 55
HpyCH4III ACNGT 1 cut(s) 157
HpyCH4IV ACGT 1 cut(s) 102
HpyF10VI GCNNNNNNNGC 4 cut(s) 17, 142, 288, 291
HpySE526I ACGT 1 cut(s) 102
HspAI GCGC 2 cut(s) 261, 289
Kzo9I GATC 2 cut(s) 225, 229
LmnI GCTCC 3 cut(s) 93, 189, 268
Lsp1109I GCAGC 1 cut(s) 145
MaeII ACGT 1 cut(s) 102
MaeIII GTNAC 2 cut(s) 55, 103
MalI GATC 2 cut(s) 227, 231
MboI GATC 2 cut(s) 225, 229
MhlI GDGCHC 4 cut(s) 22, 45, 90, 186
MlyI GAGTC 1 cut(s) 74
MnlI CCTC 4 cut(s) 22, 107, 218, 222
MspI CCGG 2 cut(s) 16, 22
MspR9I CCNGG 4 cut(s) 17, 22, 23, 50
MvaI CCWGG 1 cut(s) 50
MvnI CGCG 3 cut(s) 111, 261, 294
MwoI GCNNNNNNNGC 4 cut(s) 17, 142, 288, 291
NciI CCSGG 3 cut(s) 17, 22, 23
NdeII GATC 2 cut(s) 225, 229
NlaIV GGNNCC 4 cut(s) 20, 33, 78, 159
NmeAIII GCCGAG 1 cut(s) 88
NmuCI GTSAC 2 cut(s) 55, 103
PcsI WCGNNNNNNNCGW 2 cut(s) 108, 133
PkrI GCNGC 4 cut(s) 132, 135, 284, 293
PleI GAGTC 1 cut(s) 73
PpsI GAGTC 1 cut(s) 73
PpuMI RGGWCCY 1 cut(s) 31
Psp5II RGGWCCY 1 cut(s) 31
Psp6I CCWGG 1 cut(s) 48
PspFI CCCAGC 1 cut(s) 141
PspGI CCWGG 1 cut(s) 48
PspN4I GGNNCC 4 cut(s) 20, 33, 78, 159
PspOMI GGGCCC 1 cut(s) 18
PspPI GGNCC 3 cut(s) 18, 19, 31
PspPPI RGGWCCY 1 cut(s) 31
RsaI GTAC 1 cut(s) 201
RsaNI GTAC 1 cut(s) 200
SatI GCNGC 4 cut(s) 131, 134, 283, 292
Sau3AI GATC 2 cut(s) 225, 229
Sau96I GGNCC 3 cut(s) 18, 19, 31
SchI GAGTC 1 cut(s) 74
ScrFI CCNGG 4 cut(s) 17, 22, 23, 50
SduI GDGCHC 4 cut(s) 22, 45, 90, 186
SetI ASST 6 cut(s) 33, 54, 85, 105, 147, 192
SfcI CTRYAG 1 cut(s) 36
SinI GGWCC 1 cut(s) 31
SmaI CCCGGG 1 cut(s) 23
SsiI CCGC 7 cut(s) 60, 109, 130, 266, 280, 283, 292
StyD4I CCNGG 4 cut(s) 15, 20, 21, 48
TaaI ACNGT 1 cut(s) 157
TaiI ACGT 1 cut(s) 105
TaqI TCGA 1 cut(s) 228
TatI WGTACW 1 cut(s) 199
TauI GCSGC 3 cut(s) 133, 285, 294
TscAI CASTG 1 cut(s) 186
TseFI GTSAC 2 cut(s) 55, 103
TseI GCWGC 1 cut(s) 133
Tsp45I GTSAC 2 cut(s) 55, 103
TspGWI ACGGA 2 cut(s) 58, 185
TspMI CCCGGG 1 cut(s) 21
TspRI CASTG 1 cut(s) 186
VpaK11BI GGWCC 1 cut(s) 31
XmaI CCCGGG 1 cut(s) 21
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.