Rw0G004510

calmodulin-binding transcription activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Contig00169
OG0001204
Physical Location & Seq
Forward (+)
30289 .. 38970
8682 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw0G004510.1

Sequence Viewer

Length: 3051 bp
ATGGCTGAGATGAGAAAATACATCCCCAATCAGCAACTCGACCTTGTCCAGATATTGCAAGAATCACAGCAGCGTTGGCTCCGGCCAACTGAAATTTGTGAAATACTGCGCAACTACCAAAGGTTTCAGTTAACCCCAGATCCACCCGTCAGGCCTCCAGCTGGTTCTTTGTTCCTCTTTGATCGTAAAGCACTTCGGTATTTTCGTAAAGATGGTCACCGCTGGAGGAAGAAAAAGGATGGAAAGACTGTCAAAGAAGCGCATGAAAAGTTGAAGGCTGGTAGTGTAGACGTTCTTCACTGTTACTATGCCCATGGGGAGGACAATGAGAATTTTCAGCGGAGGAGTTATTGGATGCTTGATACTCATTTACAACACATTGTTCTTGTGCATTACAGAATGGTTGAGGGGAGCAAGTCTGGTGTCTCCCGTGTGCTGGTTGATCCAGGATCACAGGTTGGAAGCCCTCAAACTGCTGCAGCACCTTGCTCTGCACAAGCAGACTCACCTGCCCCTACAGTTCAAACATCTTTTGCTTCAAGTCCAATCAAAGTTGACTGGAATGGACAAACATTATCTACAGAATTTGAGGATGTGGATTCTCAGGGTGATGCTAGAGCTTCATCTCTTGCTCAACCTATGCCTGGTTCTGTTTTGAACAATGCTTGTCTACAGTTACCAGAAGTTGGAAGACTTTCTGAGTCATTCAGGAATCCTTCTGGTACATGGTATGCCGGACCTAAAGATTATCAAAGTGCTGGTTCATCTGACTGGGCCATGAACAGATCAACAAGAGAGTCCAGCCTGCATGACCAAAATCTTTTTGTTGAAGACATAAAGAAAAATTTCTTTGAAGAACTTAATGGATCGGACTCCATAACTCATAAGCTAACAGATGCTAGACTGGATGGTAATAGGAAAGGCAAAGATGTAACTTGTGAAGATAGGTTGACCAATGACATAGATGTGCAGGCTGTCACAACCCCTTCCCAGAGAGAAGCTCAGGTTGCAAAGGAATTTGACTTCAGTCTGTTTCATTCCCAGTTTCAGGGCCATTCTGATCCTCACACAGTAGCTTTTTCTACTGCCCAAGTTGAGAAGAGTTCTGGAGATGGTGGTGTGCGTAGAGGTGAACTAGTAGAGCTGAAGAAACTCGACAGCTTTGGTAGATGGATGGATAAAGAAATTGGTGTGGATTGTGATGATTCGTTGATGGCTTCAGACTCTGGAAATTACTGGAATACACTTGAGGCTGAGAATGGTGATAGGGAAGTTTCCAGTTTATCAGGCCATATGCAGCTAGATGTTGATTCACTTGGCCCTTCTCTTTCCCAGGAACAACTATTTAGTATATGTGATTTTTCTCCAGATTGGTCTTATTCAGGAACTGAAACAAAGGTTTTAATAGTTGGAAGATTTTTGGGAGGCAAGAAGAATTTTACTGAGACCAAGTGGGGATGCATGTTTGGTGAAATAGAGGTTCCTGCTGAAGTTCTGACTGATAATGTTATTCGATGTCAAACTCCTTTACATGCTCCTGGGTGTGTTCCATTCTATGTGACTTGCAGAAATAGGTTGGCCTGCAGTGAGGTGAGGGAGTTTGAATATAGAGAAAAACCTATCGGGATATCTCTAAACAGCTCTAGAGAAGATGAATTGCGCTTTCAGATTCGTCTTGCAAAATTGTTGAATTTGGGATCAGAAAGGAAGTGGTTGGAATGTTCTGCTTTGGATTGTGATAAATGTAAGCTCAAAAGTTCCATATGTTCAATAAGAAGCAACAGCGGGAGTGATTGGATGATAGCTGATGGAGATTCTATGGCATGCAAACGTGATCAGTTGACCCACATAGATGTATTGATTCAGAATTTGTTGAAGGATAGACTTTTTGAATGGCTGGTATATAAAGTTCATGAAGAAGGTAAAGGACCACATGTTTTAGATAATGAAGGCCAAGGCGTTCTACATTTGGCTGCTGCTCTTGGTTATGAATGGGCAATGGGTCTCATAGTTGCTGCTGGTGTCAGTCCTAATTTCAGAGATGCCCGTGGAAGAACGGGACTTCACTGGGCATCATATTTTGGAAGAGAGGAAACAGTGATCACATTACTTGGATTGGGTGCTGCTCCAGGTGCTGTTGAGGACCCAACGCCAGCATTTCCTGGAGGACAAACAGCCGCTGATCTAGCCTCAAGTAGAGGCCACAAAGGGATTGCTGGATATTTGGCAGAAGCTGATCTAACCAGTCACCTGTCATTGTTGACCGTCAATGAGAAAACAATGGATAATGTTTCCGCAACTATTGCAGCTGAAAAGGCTATCGAGATTTCAGAAGCTACAACATCTGATATGACGGTAGATGATGAAAATTCTCTGAAAGGTTCTCTAGAAGCAGTTAGGAAATCAGCCCATGCGGTTGCTCTGATTCAAGCAACTTTCCGTGCTCGTTCATTCCGCCAGAGACAATTAACTCAGAGCAGCATTGATATTCCTGAAGCTTCTATTGACCTAGTTGCACTTGGTTCCTTGAAGAGAGTTCAAAATTTCAGTCACTATGAAGATTATCTGCATTCTGCAGCAGCTTTGAAGATACAAAGAAAATATCGTGGTTGGAAGGGGAGAAAGGAATTTTTGAAGATACGTAACCGTATTGTCAAACTTCAGGCTCATGTGAGGGGACATCAGGTTCGTAAGAATTACAAAAAGCTTGTTTGGTCTGTTGGTATAATGGAGAAAGTGATATTGCGTTGGAGGCGGAAACGGCCTGGTTTGCGAGGATTTCGGGTGGAAAAGGCTATTGAAGATGTATCTTCAGAGAATAAAAGAAGTGATGACTATGAATTTTTGAGTGTTGGGAGGAAGCAGAAATTTGCTGGAGTTGAGAAAGCTCTTGCAAGGGTACAGTCCATGTCTCGTCACCCTGAAGCACGTGAACAGTATATGAGGTTACAGTCAAAGTTTGAGAAGCTTAAGATGATACCAAAAGAAAATGAATCAAGTGAGAATTGCAGCCATGAAATGCTTAAAAGAAAAGTTAATGGGTGCCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000981 GO:0000982 GO:0001077 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009409 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010150 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031349 GO:0032501 GO:0032502 GO:0032774 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070417 GO:0071704 GO:0080090 GO:0080134 GO:0090304 GO:0090693 GO:0097159 GO:0097659 GO:0098542 GO:0099402 GO:0140110 GO:1900367 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000068 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 CG-1 IQ TIG

Protein Analysis

1016

Amino Acids

114.09

Weight (kDa)

7.57

Isoelectric Point (pI)

42.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CG-1 PF03859 22 - 133 1.8e-49 CG-1 domain
Ank_2 PF12796 630 - 708 4.7e-06 Ankyrin repeats (3 copies)
Ank_2 PF12796 655 - 747 7.6e-07 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 517
Acc16I TGCGCA 1 cut(s) 110
Acc36I ACCTGC 1 cut(s) 517
AccB1I GGYRCC 1 cut(s) 3042
AccB7I CCANNNNNTGG 1 cut(s) 686
AccI GTMKAC 2 cut(s) 288, 670
AciI CCGC 8 cut(s) 220, 340, 1785, 2178, 2295, 2414, 2455, 2755
AclWI GGATC 6 cut(s) 134, 437, 457, 874, 1055, 1705
AcoI YGGCCR 1 cut(s) 83
AcuI CTGAAG 8 cut(s) 1009, 1166, 1203, 1509, 2514, 2645, 2796, 2943
AcvI CACGTG 1 cut(s) 2930
AfaI GTAC 2 cut(s) 724, 2901
AfiI CCNNNNNNNGG 6 cut(s) 161, 319, 436, 644, 686, 1048
AflII CTTAAG 1 cut(s) 2969
AflIII ACRYGT 1 cut(s) 1933
AhlI ACTAGT 1 cut(s) 1135
AjnI CCWGG 7 cut(s) 445, 643, 1332, 1537, 2128, 2161, 2764
AloI GAACNNNNNNTCC 2 cut(s) 2670, 2702
Alw21I GWGCWC 1 cut(s) 2446
Alw26I GTCTC 5 cut(s) 430, 1439, 2009, 2455, 2916
AlwI GGATC 6 cut(s) 134, 437, 457, 874, 1055, 1705
AlwNI CAGNNNCTG 5 cut(s) 1226, 1388, 2135, 2180, 2234
ArsI GACNNNNNNTTYG 4 cut(s) 2533, 2565, 2670, 2702
Asp700I GAANNNNTTC 2 cut(s) 694, 845
AspLEI GCGC 3 cut(s) 111, 262, 1662
AspS9I GGNCC 6 cut(s) 737, 774, 1051, 1319, 1928, 2143
AsuHPI GGTGA 9 cut(s) 209, 498, 620, 1142, 1274, 1481, 1603, 2240, 2909
AvaII GGWCC 3 cut(s) 737, 1928, 2143
BanI GGYRCC 1 cut(s) 3042
BarI GAAGNNNNNNTAC 2 cut(s) 279, 311
BbrPI CACGTG 1 cut(s) 2930
BbsI GAAGAC 2 cut(s) 697, 837
Bbv12I GWGCWC 1 cut(s) 2446
BccI CCATC 8 cut(s) 206, 233, 902, 1106, 1164, 1168, 1207, 1802
BceAI ACGGC 1 cut(s) 2777
BciT130I CCWGG 7 cut(s) 447, 645, 1334, 1539, 2130, 2163, 2766
BclI TGATCA 2 cut(s) 1834, 2100
BcoDI GTCTC 5 cut(s) 430, 1439, 2009, 2455, 2916
BcuI ACTAGT 1 cut(s) 1135
BfaI CTAG 9 cut(s) 615, 900, 1136, 1301, 1644, 2186, 2387, 2510, 3049
BfmI CTRYAG 6 cut(s) 477, 516, 579, 671, 1582, 2574
BfrI CTTAAG 1 cut(s) 2969
BfuAI ACCTGC 1 cut(s) 517
Bme1390I CCNGG 7 cut(s) 447, 645, 1334, 1539, 2130, 2163, 2766
Bme18I GGWCC 3 cut(s) 737, 1928, 2143
BmgT120I GGNCC 6 cut(s) 737, 774, 1051, 1319, 1928, 2143
BmiI GGNNCC 5 cut(s) 80, 1482, 2145, 2524, 3044
BmrFI CCNGG 7 cut(s) 447, 645, 1334, 1539, 2130, 2163, 2766
BmrI ACTGGG 3 cut(s) 781, 1036, 2077
BmsI GCATC 6 cut(s) 345, 601, 886, 1448, 2032, 2081
BmuI ACTGGG 3 cut(s) 781, 1036, 2077
BoxI GACNNNNGTC 1 cut(s) 1026
BpiI GAAGAC 2 cut(s) 697, 837
BplI GAGNNNNNCTC 2 cut(s) 985, 1017
BpmI CTGGAG 7 cut(s) 141, 244, 1128, 1350, 2112, 2184, 2895
Bpu10I CCTNAGC 1 cut(s) 1002
BpuEI CTTGAG 2 cut(s) 1268, 2176
BsaAI YACGTR 2 cut(s) 2642, 2930
BsaI GGTCTC 2 cut(s) 1439, 2009
BsaJI CCNNGG 5 cut(s) 313, 1332, 1538, 1954, 2047
Bsc4I CCNNNNNNNGG 6 cut(s) 161, 319, 436, 644, 686, 1048
Bse1I ACTGG 8 cut(s) 563, 776, 909, 1042, 1241, 1278, 2072, 2244
Bse3DI GCAATG 1 cut(s) 2004
BseBI CCWGG 7 cut(s) 447, 645, 1334, 1539, 2130, 2163, 2766
BseDI CCNNGG 5 cut(s) 313, 1332, 1538, 1954, 2047
BseGI GGATG 8 cut(s) 21, 244, 360, 598, 913, 1179, 1463, 1803
BseLI CCNNNNNNNGG 6 cut(s) 161, 319, 436, 644, 686, 1048
BseMI GCAATG 1 cut(s) 2004
BseMII CTCAG 6 cut(s) 617, 690, 1016, 1245, 1434, 2486
BseNI ACTGG 8 cut(s) 563, 776, 909, 1042, 1241, 1278, 2072, 2244
BseRI GAGGAG 1 cut(s) 358
BsgI GTGCAG 2 cut(s) 477, 989
BshNI GGYRCC 1 cut(s) 3042
BsiHKAI GWGCWC 1 cut(s) 2446
BsiSI CCGG 2 cut(s) 82, 735
BslFI GGGAC 2 cut(s) 2073, 2691
BslI CCNNNNNNNGG 6 cut(s) 161, 319, 436, 644, 686, 1048
BsmAI GTCTC 5 cut(s) 430, 1439, 2009, 2455, 2916
BsmFI GGGAC 2 cut(s) 2073, 2691
BsmI GAATGC 1 cut(s) 2569
Bso31I GGTCTC 2 cut(s) 1439, 2009
Bsp1286I GDGCHC 1 cut(s) 2446
Bsp19I CCATGG 1 cut(s) 313
BspACI CCGC 8 cut(s) 220, 340, 1785, 2178, 2295, 2414, 2455, 2755
BspCNI CTCAG 6 cut(s) 616, 691, 1015, 1246, 1435, 2485
BspHI TCATGA 1 cut(s) 1912
BspLI GGNNCC 5 cut(s) 80, 1482, 2145, 2524, 3044
BspMAI CTGCAG 3 cut(s) 481, 1586, 2578
BspMI ACCTGC 1 cut(s) 517
BspPI GGATC 6 cut(s) 134, 437, 457, 874, 1055, 1705
BspT107I GGYRCC 1 cut(s) 3042
BspTI CTTAAG 1 cut(s) 2969
BspTNI GGTCTC 2 cut(s) 1439, 2009
BsrDI GCAATG 1 cut(s) 2004
BsrI ACTGG 8 cut(s) 563, 776, 909, 1042, 1241, 1278, 2072, 2244
BssECI CCNNGG 5 cut(s) 313, 1332, 1538, 1954, 2047
BssT1I CCWWGG 2 cut(s) 313, 1954
Bst2UI CCWGG 7 cut(s) 447, 645, 1334, 1539, 2130, 2163, 2766
Bst6I CTCTTC 3 cut(s) 1094, 2080, 2525
BstAFI CTTAAG 1 cut(s) 2969
BstAPI GCANNNNNTGC 1 cut(s) 2303
BstBAI YACGTR 2 cut(s) 2642, 2930
BstC8I GCNNGC 5 cut(s) 806, 972, 1582, 1825, 2154
BstDEI CTNAG 7 cut(s) 6, 603, 699, 1002, 1254, 1443, 2472
BstDSI CCRYGG 2 cut(s) 313, 2047
BstEII GGTNACC 1 cut(s) 215
BstF5I GGATG 8 cut(s) 21, 244, 360, 598, 913, 1179, 1463, 1803
BstHHI GCGC 3 cut(s) 111, 262, 1662
BstMAI GTCTC 5 cut(s) 430, 1439, 2009, 2455, 2916
BstMWI GCNNNNNNNGC 9 cut(s) 76, 1007, 2132, 2186, 2303, 2315, 2752, 2761, 2770
BstNI CCWGG 7 cut(s) 447, 645, 1334, 1539, 2130, 2163, 2766
BstNSI RCATGY 4 cut(s) 1465, 1535, 1827, 1937
BstPAI GACNNNNGTC 1 cut(s) 1026
BstPI GGTNACC 1 cut(s) 215
BstSCI CCNGG 7 cut(s) 445, 643, 1332, 1537, 2128, 2161, 2764
BstSFI CTRYAG 6 cut(s) 477, 516, 579, 671, 1582, 2574
BstSNI TACGTA 1 cut(s) 2642
BstV2I GAAGAC 2 cut(s) 697, 837
BstX2I RGATCY 1 cut(s) 139
BstYI RGATCY 1 cut(s) 139
BtgI CCRYGG 2 cut(s) 313, 2047
BtsCI GGATG 8 cut(s) 21, 244, 360, 598, 913, 1179, 1463, 1803
BtsI GCAGTG 1 cut(s) 1591
BtsIMutI CAGTG 4 cut(s) 298, 1591, 2065, 2103
BveI ACCTGC 1 cut(s) 517
Cac8I GCNNGC 5 cut(s) 806, 972, 1582, 1825, 2154
CaiI CAGNNNCTG 5 cut(s) 1226, 1388, 2135, 2180, 2234
CciI TCATGA 1 cut(s) 1912
CfoI GCGC 3 cut(s) 111, 262, 1662
Cfr13I GGNCC 6 cut(s) 737, 774, 1051, 1319, 1928, 2143
Csp6I GTAC 2 cut(s) 723, 2900
CviQI GTAC 2 cut(s) 723, 2900
DdeI CTNAG 7 cut(s) 6, 603, 699, 1002, 1254, 1443, 2472
EaeI YGGCCR 1 cut(s) 83
Eam1104I CTCTTC 3 cut(s) 1094, 2080, 2525
EarI CTCTTC 3 cut(s) 1094, 2080, 2525
EciI GGCGGA 2 cut(s) 2444, 2770
Eco105I TACGTA 1 cut(s) 2642
Eco130I CCWWGG 2 cut(s) 313, 1954
Eco147I AGGCCT 1 cut(s) 154
Eco31I GGTCTC 2 cut(s) 1439, 2009
Eco32I GATATC 1 cut(s) 1629
Eco47I GGWCC 3 cut(s) 737, 1928, 2143
Eco57I CTGAAG 8 cut(s) 1009, 1166, 1203, 1509, 2514, 2645, 2796, 2943
Eco72I CACGTG 1 cut(s) 2930
Eco91I GGTNACC 1 cut(s) 215
EcoO109I RGGNCCY 1 cut(s) 2143
EcoO65I GGTNACC 1 cut(s) 215
EcoRII CCWGG 7 cut(s) 445, 643, 1332, 1537, 2128, 2161, 2764
EcoRV GATATC 1 cut(s) 1629
EcoT14I CCWWGG 2 cut(s) 313, 1954
EcoT22I ATGCAT 1 cut(s) 1463
ErhI CCWWGG 2 cut(s) 313, 1954
FaqI GGGAC 2 cut(s) 2073, 2691
FauI CCCGC 1 cut(s) 1778
FauNDI CATATG 2 cut(s) 1293, 1763
FbaI TGATCA 2 cut(s) 1834, 2100
FblI GTMKAC 2 cut(s) 288, 670
FokI GGATG 8 cut(s) 8, 251, 367, 605, 920, 1186, 1470, 1810
FspBI CTAG 9 cut(s) 615, 900, 1136, 1301, 1644, 2186, 2387, 2510, 3049
FspI TGCGCA 1 cut(s) 110
GlaI GCGC 3 cut(s) 110, 261, 1661
GsuI CTGGAG 7 cut(s) 141, 244, 1128, 1350, 2112, 2184, 2895
HapII CCGG 2 cut(s) 82, 735
HhaI GCGC 3 cut(s) 111, 262, 1662
Hin6I GCGC 3 cut(s) 109, 260, 1660
HinP1I GCGC 3 cut(s) 109, 260, 1660
HincII GTYRAC 5 cut(s) 132, 556, 951, 1842, 2262
HindII GTYRAC 5 cut(s) 132, 556, 951, 1842, 2262
HindIII AAGCTT 3 cut(s) 2496, 2705, 2966
HpaI GTTAAC 1 cut(s) 132
HpaII CCGG 2 cut(s) 82, 735
HphI GGTGA 9 cut(s) 209, 498, 620, 1142, 1274, 1481, 1603, 2240, 2909
Hpy166II GTNNAC 9 cut(s) 132, 289, 556, 671, 951, 1133, 1842, 2262, 2933
Hpy8I GTNNAC 9 cut(s) 132, 289, 556, 671, 951, 1133, 1842, 2262, 2933
HpyAV CCTTC 8 cut(s) 268, 726, 996, 1332, 1870, 1913, 1943, 2608
HpyCH4IV ACGT 4 cut(s) 291, 1831, 2641, 2929
HpyF10VI GCNNNNNNNGC 9 cut(s) 76, 1007, 2132, 2186, 2303, 2315, 2752, 2761, 2770
HpyF3I CTNAG 7 cut(s) 6, 603, 699, 1002, 1254, 1443, 2472
HpySE526I ACGT 4 cut(s) 291, 1831, 2641, 2929
HspAI GCGC 3 cut(s) 109, 260, 1660
Ksp22I TGATCA 2 cut(s) 1834, 2100
KspAI GTTAAC 1 cut(s) 132
LmnI GCTCC 4 cut(s) 84, 411, 1540, 2131
LweI GCATC 6 cut(s) 345, 601, 886, 1448, 2032, 2081
MaeI CTAG 9 cut(s) 615, 900, 1136, 1301, 1644, 2186, 2387, 2510, 3049
MaeII ACGT 4 cut(s) 291, 1831, 2641, 2929
MflI RGATCY 1 cut(s) 139
MhlI GDGCHC 1 cut(s) 2446
MlyI GAGTC 5 cut(s) 497, 710, 806, 866, 1217
MmeI TCCRAC 6 cut(s) 439, 667, 1390, 1695, 2591, 2729
Mph1103I ATGCAT 1 cut(s) 1463
MroXI GAANNNNTTC 2 cut(s) 694, 845
MseI TTAA 7 cut(s) 131, 861, 1403, 2468, 2970, 3024, 3036
MslI CAYNNNNRTG 2 cut(s) 965, 1851
MspA1I CMGCKG 6 cut(s) 161, 222, 340, 1785, 2180, 2309
MspCI CTTAAG 1 cut(s) 2969
MspI CCGG 2 cut(s) 82, 735
MspR9I CCNGG 7 cut(s) 447, 645, 1334, 1539, 2130, 2163, 2766
Mva1269I GAATGC 1 cut(s) 2569
MvaI CCWGG 7 cut(s) 447, 645, 1334, 1539, 2130, 2163, 2766
MwoI GCNNNNNNNGC 9 cut(s) 76, 1007, 2132, 2186, 2303, 2315, 2752, 2761, 2770
NcoI CCATGG 1 cut(s) 313
NdeI CATATG 2 cut(s) 1293, 1763
NlaIV GGNNCC 5 cut(s) 80, 1482, 2145, 2524, 3044
NmuCI GTSAC 6 cut(s) 215, 976, 1558, 2246, 2549, 2915
NsbI TGCGCA 1 cut(s) 110
NsiI ATGCAT 1 cut(s) 1463
NspI RCATGY 4 cut(s) 1465, 1535, 1827, 1937
PaeI GCATGC 1 cut(s) 1827
PagI TCATGA 1 cut(s) 1912
PaqCI CACCTGC 1 cut(s) 517
PceI AGGCCT 1 cut(s) 154
PciI ACATGT 1 cut(s) 1933
PcsI WCGNNNNNNNCGW 1 cut(s) 202
PctI GAATGC 1 cut(s) 2569
PdmI GAANNNNTTC 2 cut(s) 694, 845
PflFI GACNNNGTC 1 cut(s) 44
PflMI CCANNNNNTGG 1 cut(s) 686
PfoI TCCNGGA 2 cut(s) 445, 2161
PleI GAGTC 5 cut(s) 497, 709, 805, 866, 1217
PmaCI CACGTG 1 cut(s) 2930
PmlI CACGTG 1 cut(s) 2930
PpsI GAGTC 5 cut(s) 497, 709, 805, 866, 1217
Ppu21I YACGTR 2 cut(s) 2642, 2930
PpuMI RGGWCCY 1 cut(s) 2143
PscI ACATGT 1 cut(s) 1933
PshAI GACNNNNGTC 1 cut(s) 1026
Psp5II RGGWCCY 1 cut(s) 2143
Psp6I CCWGG 7 cut(s) 445, 643, 1332, 1537, 2128, 2161, 2764
PspCI CACGTG 1 cut(s) 2930
PspEI GGTNACC 1 cut(s) 215
PspGI CCWGG 7 cut(s) 445, 643, 1332, 1537, 2128, 2161, 2764
PspN4I GGNNCC 5 cut(s) 80, 1482, 2145, 2524, 3044
PspPI GGNCC 6 cut(s) 737, 774, 1051, 1319, 1928, 2143
PspPPI RGGWCCY 1 cut(s) 2143
PsrI GAACNNNNNNTAC 2 cut(s) 1893, 1925
PstI CTGCAG 3 cut(s) 481, 1586, 2578
PstNI CAGNNNCTG 5 cut(s) 1226, 1388, 2135, 2180, 2234
PsuI RGATCY 1 cut(s) 139
PsyI GACNNNGTC 1 cut(s) 44
PvuII CAGCTG 2 cut(s) 161, 2309
RsaI GTAC 2 cut(s) 724, 2901
RsaNI GTAC 2 cut(s) 723, 2900
RseI CAYNNNNRTG 2 cut(s) 965, 1851
SaqAI TTAA 7 cut(s) 131, 861, 1403, 2468, 2970, 3024, 3036
Sau96I GGNCC 6 cut(s) 737, 774, 1051, 1319, 1928, 2143
SchI GAGTC 5 cut(s) 497, 710, 806, 866, 1217
ScrFI CCNGG 7 cut(s) 447, 645, 1334, 1539, 2130, 2163, 2766
SduI GDGCHC 1 cut(s) 2446
SfaNI GCATC 6 cut(s) 345, 601, 886, 1448, 2032, 2081
SfcI CTRYAG 6 cut(s) 477, 516, 579, 671, 1582, 2574
SinI GGWCC 3 cut(s) 737, 1928, 2143
SmiMI CAYNNNNRTG 2 cut(s) 965, 1851
SmlI CTYRAG 3 cut(s) 1247, 2191, 2969
SmoI CTYRAG 3 cut(s) 1247, 2191, 2969
SnaBI TACGTA 1 cut(s) 2642
SpeI ACTAGT 1 cut(s) 1135
SphI GCATGC 1 cut(s) 1827
SseBI AGGCCT 1 cut(s) 154
SsiI CCGC 8 cut(s) 220, 340, 1785, 2178, 2295, 2414, 2455, 2755
SspMI CTAG 9 cut(s) 615, 900, 1136, 1301, 1644, 2186, 2387, 2510, 3049
StuI AGGCCT 1 cut(s) 154
StyD4I CCNGG 7 cut(s) 445, 643, 1332, 1537, 2128, 2161, 2764
StyI CCWWGG 2 cut(s) 313, 1954
TaiI ACGT 4 cut(s) 294, 1834, 2644, 2932
TaqI TCGA 4 cut(s) 39, 1155, 1513, 2322
TauI GCSGC 1 cut(s) 2180
Tru1I TTAA 7 cut(s) 131, 861, 1403, 2468, 2970, 3024, 3036
Tru9I TTAA 7 cut(s) 131, 861, 1403, 2468, 2970, 3024, 3036
TscAI CASTG 4 cut(s) 305, 1591, 2072, 2103
TseFI GTSAC 6 cut(s) 215, 976, 1558, 2246, 2549, 2915
Tsp45I GTSAC 6 cut(s) 215, 976, 1558, 2246, 2549, 2915
TspGWI ACGGA 1 cut(s) 2429
TspRI CASTG 4 cut(s) 305, 1591, 2072, 2103
Tth111I GACNNNGTC 1 cut(s) 44
Van91I CCANNNNNTGG 1 cut(s) 686
Vha464I CTTAAG 1 cut(s) 2969
VpaK11BI GGWCC 3 cut(s) 737, 1928, 2143
XbaI TCTAGA 2 cut(s) 1643, 2386
XceI RCATGY 4 cut(s) 1465, 1535, 1827, 1937
XmiI GTMKAC 2 cut(s) 288, 670
XmnI GAANNNNTTC 2 cut(s) 694, 845
XspI CTAG 9 cut(s) 615, 900, 1136, 1301, 1644, 2186, 2387, 2510, 3049
Zsp2I ATGCAT 1 cut(s) 1463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.