MD12G1040100.v1.1

proximal promoter DNA-binding transcription activator activity, RNA polymerase II-specific

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0001204
Physical Location & Seq
Forward (+)
4348556 .. 4355041
6486 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1040100.v1.1.491

Sequence Viewer

Length: 1794 bp
ATGGCGGAGAGCAATCGCGATCTTCATCAACCAGAAAGTAGAATTCTTCAATTGAATACAAGTAGAATACTTTCTGAAGGGAAACATCGATGGCTACGTCCGCATGAAATATGTGAAGTTCTTCGGAATCCTAACGGAATTACAATTTCTAAGAAGCCTACAACCATGCCACCGAGTGGATCCATATTTCTTTTTGATAAGAAGGTGCAGAGATATTTTCGAAAAGATGGCTATAGATGGAAGAAGGCAAAAGATGGAAAGAGTACTAAGGAAGCCCATGAGAGGCTCAAGGTTGGAGGTGTTGATACATTACATTGCTACTATGCTCATGGAGAAGAAGATAAAAATTTTCAACGACGCAGTTACTGGATGATTGATAAGTATCTCTCGCATATAGTTCTTGTCCACTATCTAGACGTAAAGGGAGATACAATGGGGGTTGGATACATTAAAGAGACTCAGACTGCGGAAGTTGTTCAGCATTCCCGTAAAACTAACAAAATTACACCCAATTCTGAGATGGACTGTTCTGCTGCCTTGTCTTGTTCAAATAACGGTGTCTCTTCTCAAGAAACTGGACTCTGGATTAGGATGGTAGAGGATGCCGTGATAACTTTGTTTAGTTCAAATTCATCAAACCTTGGAGATGATGAAACAGGAAAGTCCAAGGCCAAAAATGAAGAGGAAGGGGGAAACGATATAAACCAAGTCCGGAAGCGACCCAGAGTCACTTGTGGCCAGCAGGTAGACTCAGCCACCCAAAGTCACTCATGGCTAGAGTGGATATACCCCATGGCAATTGATACAGCCATTACTGATGGTGCGACGAATGATGCAACTGCATTGAGCCAGGCACAAGAGGACGATGAAACCACCAGATTGGCTGCATTTCATATTGCAGACCCTACTGTGGCTGCAATTTCAGCCATAGGGCCAACACATCTAAATCCATATACCACAGCCAATGTAGCTGATCAGGCTCAAGACTTGCTGCCACCAGTTCTACAGCCAACTGCAACCACTGGGGTTGTTATTTCGGCCACAACCAATGAGACTGCTGCTTCCAAGTCCAGCAATGCTGCCTTGGTGCAATCGGAGCCAAACTCAAATGCCAACCAAGATTTAGCCCAAGACATAATTGAGAGTCATGAGTTTGCAGAAGTTCGGGTTATTAGCTCCCTTAAAAGACTCCATGAACTTTTAGGCCAAGCGTCTGCACCCAATCCAGTGGCCAGCTCCTCCATTTCTACTCCTACCATAGACTCGCAAGCCATGGCAAAAACCAGAGCTACTTTAGAAATGGACTTTATTACTATGGCTGGTGAGGTTCAACGAGCTCACATGATTTCAGGCATCCAGAGCTTGGTAGATTCTAATTGCTTTCCCACCGGCACTGGACCAATGATTATGGCTATGTTGAAGCAGCTAGAGCAATCTGTTCCAGTCTACTCCAAAGCCTATTCTGAGTATGATATGGGTAAAAACTTGACTGTCCGAGTCGAAGCTATGAAACAGCTTCTTCAACCTAAGGTTCAGTCTTGTGATAGCAAGGAGAGAGAATTTCAAGACCTTGCGAAATGGAAAATGAAGATTGAAGCCAAGCAGGCTGCAATACTTGCTGAAATTGATGGCATCGTCAAAGAGACTGAAACGATGCAGGCACAACTCAAGAAATTGCTTTTACAGCAAGATCAATTTAAAGAAGATGAAGGTTATCTCAAGGCCATTTTGGACAATGGAGATGGCCTCTGGACAAAGTTTAAGAACATAATTCACCAGCACCTCCCCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3 CG-1 LRR_3 NB-ARC TIR

Protein Analysis

598

Amino Acids

66.28

Weight (kDa)

5.98

Isoelectric Point (pI)

42.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CG-1 PF03859 28 - 139 1.6e-45 CG-1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 733
AccB7I CCANNNNNTGG 2 cut(s) 176, 1363
AccI GTMKAC 2 cut(s) 747, 1446
AccII CGCG 1 cut(s) 18
AccIII TCCGGA 1 cut(s) 711
AciI CCGC 3 cut(s) 5, 101, 467
AclWI GGATC 2 cut(s) 174, 187
AcoI YGGCCR 3 cut(s) 736, 1038, 1230
AcsI RAATTY 4 cut(s) 42, 346, 628, 1559
AcuI CTGAAG 1 cut(s) 96
AdeI CACNNNGTG 1 cut(s) 176
AfaI GTAC 1 cut(s) 265
AfiI CCNNNNNNNGG 4 cut(s) 176, 282, 910, 1363
AhdI GACNNNNNGTC 1 cut(s) 725
AjnI CCWGG 1 cut(s) 849
AluBI AGCT 9 cut(s) 971, 1176, 1236, 1289, 1337, 1362, 1426, 1505, 1516
AluI AGCT 9 cut(s) 971, 1176, 1236, 1289, 1337, 1362, 1426, 1505, 1516
Alw21I GWGCWC 1 cut(s) 1339
Alw26I GTCTC 4 cut(s) 449, 565, 1046, 1637
AlwI GGATC 2 cut(s) 174, 187
AlwNI CAGNNNCTG 1 cut(s) 366
Aor13HI TCCGGA 1 cut(s) 711
AoxI GGCC 8 cut(s) 669, 736, 932, 1038, 1204, 1230, 1722, 1744
ApeKI GCWGC 8 cut(s) 533, 884, 914, 991, 1058, 1079, 1423, 1607
ApoI RAATTY 4 cut(s) 42, 346, 628, 1559
Asp700I GAANNNNTTC 3 cut(s) 70, 120, 474
AspS9I GGNCC 2 cut(s) 932, 1397
AsuHPI GGTGA 2 cut(s) 1334, 1766
AsuII TTCGAA 1 cut(s) 220
AvaII GGWCC 1 cut(s) 1397
AxyI CCTNAGG 1 cut(s) 1527
BalI TGGCCA 2 cut(s) 738, 1232
BamHI GGATCC 1 cut(s) 179
BanII GRGCYC 1 cut(s) 1339
Bbv12I GWGCWC 1 cut(s) 1339
BbvI GCAGC 8 cut(s) 520, 871, 901, 978, 1045, 1066, 1435, 1594
BccI CCATC 9 cut(s) 84, 221, 231, 248, 514, 586, 812, 1622, 1736
BceAI ACGGC 1 cut(s) 590
BciT130I CCWGG 1 cut(s) 851
BciVI GTATCC 1 cut(s) 437
BclI TGATCA 1 cut(s) 973
BcoDI GTCTC 4 cut(s) 449, 565, 1046, 1637
BfaI CTAG 3 cut(s) 413, 776, 1427
BfmI CTRYAG 2 cut(s) 232, 1004
BfuAI ACCTGC 1 cut(s) 733
BfuI GTATCC 1 cut(s) 437
BglI GCCNNNNNGGC 1 cut(s) 1604
BisI GCNGC 8 cut(s) 534, 885, 915, 992, 1059, 1080, 1424, 1608
BlsI GCNGC 8 cut(s) 535, 886, 916, 993, 1060, 1081, 1425, 1609
BmcAI AGTACT 1 cut(s) 265
Bme1390I CCNGG 1 cut(s) 851
Bme18I GGWCC 1 cut(s) 1397
BmeRI GACNNNNNGTC 1 cut(s) 725
BmgT120I GGNCC 2 cut(s) 932, 1397
BmiI GGNNCC 2 cut(s) 181, 1098
BmrFI CCNGG 1 cut(s) 851
BmrI ACTGGG 1 cut(s) 1032
BmsI GCATC 5 cut(s) 592, 823, 1362, 1641, 1644
BmuI ACTGGG 1 cut(s) 1032
BplI GAGNNNNNCTC 4 cut(s) 1088, 1120, 1731, 1763
Bpu14I TTCGAA 1 cut(s) 220
BpuEI CTTGAG 5 cut(s) 272, 552, 966, 1652, 1703
Bsa29I ATCGAT 1 cut(s) 88
BsaBI GATNNNNATC 2 cut(s) 24, 381
BsaJI CCNNGG 5 cut(s) 640, 666, 792, 1083, 1272
BsaWI WCCGGW 1 cut(s) 711
Bsc4I CCNNNNNNNGG 4 cut(s) 176, 282, 910, 1363
Bse118I RCCGGY 1 cut(s) 1388
Bse1I ACTGG 7 cut(s) 371, 580, 998, 1027, 1226, 1399, 1442
Bse21I CCTNAGG 1 cut(s) 1527
Bse3DI GCAATG 2 cut(s) 313, 1081
Bse8I GATNNNNATC 2 cut(s) 24, 381
BseAI TCCGGA 1 cut(s) 711
BseBI CCWGG 1 cut(s) 851
BseCI ATCGAT 1 cut(s) 88
BseDI CCNNGG 5 cut(s) 640, 666, 792, 1083, 1272
BseGI GGATG 4 cut(s) 375, 597, 607, 1353
BseJI GATNNNNATC 2 cut(s) 24, 381
BseLI CCNNNNNNNGG 4 cut(s) 176, 282, 910, 1363
BseMI GCAATG 2 cut(s) 313, 1081
BseMII CTCAG 4 cut(s) 473, 507, 765, 1455
BseNI ACTGG 7 cut(s) 371, 580, 998, 1027, 1226, 1399, 1442
BseRI GAGGAG 1 cut(s) 1228
BseXI GCAGC 8 cut(s) 520, 871, 901, 978, 1045, 1066, 1435, 1594
BsgI GTGCAG 2 cut(s) 227, 1200
Bsh1236I CGCG 1 cut(s) 18
BshFI GGCC 8 cut(s) 671, 738, 934, 1040, 1206, 1232, 1724, 1746
BshVI ATCGAT 1 cut(s) 88
BsiHKAI GWGCWC 1 cut(s) 1339
BsiSI CCGG 2 cut(s) 712, 1389
BslI CCNNNNNNNGG 4 cut(s) 176, 282, 910, 1363
BsmAI GTCTC 4 cut(s) 449, 565, 1046, 1637
BsmI GAATGC 1 cut(s) 481
BsnI GGCC 8 cut(s) 671, 738, 934, 1040, 1206, 1232, 1724, 1746
Bsp119I TTCGAA 1 cut(s) 220
Bsp1286I GDGCHC 1 cut(s) 1339
Bsp13I TCCGGA 1 cut(s) 711
Bsp143I GATC 4 cut(s) 19, 179, 973, 1690
Bsp19I CCATGG 2 cut(s) 792, 1272
Bsp68I TCGCGA 1 cut(s) 18
BspACI CCGC 3 cut(s) 5, 101, 467
BspANI GGCC 8 cut(s) 671, 738, 934, 1040, 1206, 1232, 1724, 1746
BspCNI CTCAG 4 cut(s) 472, 508, 764, 1456
BspDI ATCGAT 1 cut(s) 88
BspEI TCCGGA 1 cut(s) 711
BspFNI CGCG 1 cut(s) 18
BspHI TCATGA 1 cut(s) 1147
BspLI GGNNCC 2 cut(s) 181, 1098
BspMI ACCTGC 1 cut(s) 733
BspPI GGATC 2 cut(s) 174, 187
BspT104I TTCGAA 1 cut(s) 220
BsrDI GCAATG 2 cut(s) 313, 1081
BsrFI RCCGGY 1 cut(s) 1388
BsrI ACTGG 7 cut(s) 371, 580, 998, 1027, 1226, 1399, 1442
BssAI RCCGGY 1 cut(s) 1388
BssECI CCNNGG 5 cut(s) 640, 666, 792, 1083, 1272
BssMI GATC 4 cut(s) 19, 179, 973, 1690
BssT1I CCWWGG 5 cut(s) 640, 666, 792, 1083, 1272
Bst2UI CCWGG 1 cut(s) 851
Bst4CI ACNGT 4 cut(s) 527, 557, 910, 1492
Bst6I CTCTTC 2 cut(s) 568, 675
BstAPI GCANNNNNTGC 1 cut(s) 1616
BstBI TTCGAA 1 cut(s) 220
BstC8I GCNNGC 5 cut(s) 740, 1234, 1269, 1605, 1659
BstDEI CTNAG 7 cut(s) 150, 267, 459, 516, 751, 1464, 1527
BstDSI CCRYGG 2 cut(s) 792, 1272
BstF5I GGATG 4 cut(s) 375, 597, 607, 1353
BstFNI CGCG 1 cut(s) 18
BstKTI GATC 4 cut(s) 22, 182, 976, 1693
BstMAI GTCTC 4 cut(s) 449, 565, 1046, 1637
BstMBI GATC 4 cut(s) 19, 179, 973, 1690
BstNI CCWGG 1 cut(s) 851
BstSCI CCNGG 1 cut(s) 849
BstSFI CTRYAG 2 cut(s) 232, 1004
BstUI CGCG 1 cut(s) 18
BstV1I GCAGC 8 cut(s) 520, 871, 901, 978, 1045, 1066, 1435, 1594
BstX2I RGATCY 1 cut(s) 179
BstXI CCANNNNNNTGG 2 cut(s) 880, 1228
BstYI RGATCY 1 cut(s) 179
Bsu15I ATCGAT 1 cut(s) 88
Bsu36I CCTNAGG 1 cut(s) 1527
BsuI GTATCC 1 cut(s) 437
BsuRI GGCC 8 cut(s) 671, 738, 934, 1040, 1206, 1232, 1724, 1746
BsuTUI ATCGAT 1 cut(s) 88
BtgI CCRYGG 2 cut(s) 792, 1272
BtsCI GGATG 4 cut(s) 375, 597, 607, 1353
BtsIMutI CAGTG 3 cut(s) 1020, 1233, 1392
BtuMI TCGCGA 1 cut(s) 18
BveI ACCTGC 1 cut(s) 733
Cac8I GCNNGC 5 cut(s) 740, 1234, 1269, 1605, 1659
CaiI CAGNNNCTG 1 cut(s) 366
CciI TCATGA 1 cut(s) 1147
Cfr10I RCCGGY 1 cut(s) 1388
Cfr13I GGNCC 2 cut(s) 932, 1397
ClaI ATCGAT 1 cut(s) 88
CseI GACGC 2 cut(s) 366, 1200
Csp6I GTAC 1 cut(s) 264
CspCI CAANNNNNGTGG 2 cut(s) 1009, 1044
CviQI GTAC 1 cut(s) 264
DdeI CTNAG 7 cut(s) 150, 267, 459, 516, 751, 1464, 1527
DpnI GATC 4 cut(s) 21, 181, 975, 1692
DpnII GATC 4 cut(s) 19, 179, 973, 1690
DraI TTTAAA 1 cut(s) 1699
DraIII CACNNNGTG 1 cut(s) 176
DriI GACNNNNNGTC 1 cut(s) 725
EaeI YGGCCR 3 cut(s) 736, 1038, 1230
Eam1104I CTCTTC 2 cut(s) 568, 675
Eam1105I GACNNNNNGTC 1 cut(s) 725
EarI CTCTTC 2 cut(s) 568, 675
EciI GGCGGA 1 cut(s) 20
Ecl136II GAGCTC 1 cut(s) 1337
Eco130I CCWWGG 5 cut(s) 640, 666, 792, 1083, 1272
Eco24I GRGCYC 1 cut(s) 1339
Eco47I GGWCC 1 cut(s) 1397
Eco53kI GAGCTC 1 cut(s) 1337
Eco57I CTGAAG 1 cut(s) 96
Eco81I CCTNAGG 1 cut(s) 1527
EcoICRI GAGCTC 1 cut(s) 1337
EcoRI GAATTC 1 cut(s) 42
EcoRII CCWGG 1 cut(s) 849
EcoT14I CCWWGG 5 cut(s) 640, 666, 792, 1083, 1272
EcoT38I GRGCYC 1 cut(s) 1339
ErhI CCWWGG 5 cut(s) 640, 666, 792, 1083, 1272
FbaI TGATCA 1 cut(s) 973
FblI GTMKAC 2 cut(s) 747, 1446
Fnu4HI GCNGC 8 cut(s) 534, 885, 915, 992, 1059, 1080, 1424, 1608
FokI GGATG 4 cut(s) 382, 604, 614, 1340
FriOI GRGCYC 1 cut(s) 1339
Fsp4HI GCNGC 8 cut(s) 534, 885, 915, 992, 1059, 1080, 1424, 1608
FspBI CTAG 3 cut(s) 413, 776, 1427
GluI GCNGC 8 cut(s) 534, 885, 915, 992, 1059, 1080, 1424, 1608
HaeIII GGCC 8 cut(s) 671, 738, 934, 1040, 1206, 1232, 1724, 1746
HapII CCGG 2 cut(s) 712, 1389
HgaI GACGC 2 cut(s) 366, 1200
HpaII CCGG 2 cut(s) 712, 1389
HphI GGTGA 2 cut(s) 1334, 1766
Hpy166II GTNNAC 3 cut(s) 406, 748, 1447
Hpy188I TCNGA 7 cut(s) 76, 126, 462, 517, 1096, 1465, 1496
Hpy8I GTNNAC 3 cut(s) 406, 748, 1447
Hpy99I CGWCG 2 cut(s) 360, 829
HpyAV CCTTC 5 cut(s) 71, 196, 238, 680, 1703
HpyCH4III ACNGT 4 cut(s) 527, 557, 910, 1492
HpyCH4IV ACGT 2 cut(s) 97, 417
HpyF3I CTNAG 7 cut(s) 150, 267, 459, 516, 751, 1464, 1527
HpySE526I ACGT 2 cut(s) 97, 417
Kpn2I TCCGGA 1 cut(s) 711
Ksp22I TGATCA 1 cut(s) 973
Kzo9I GATC 4 cut(s) 19, 179, 973, 1690
LmnI GCTCC 3 cut(s) 1096, 1181, 1241
Lsp1109I GCAGC 8 cut(s) 520, 871, 901, 978, 1045, 1066, 1435, 1594
LweI GCATC 5 cut(s) 592, 823, 1362, 1641, 1644
MaeI CTAG 3 cut(s) 413, 776, 1427
MaeII ACGT 2 cut(s) 97, 417
MaeIII GTNAC 3 cut(s) 362, 727, 764
MalI GATC 4 cut(s) 21, 181, 975, 1692
MboI GATC 4 cut(s) 19, 179, 973, 1690
MfeI CAATTG 2 cut(s) 50, 798
MflI RGATCY 1 cut(s) 179
MhlI GDGCHC 1 cut(s) 1339
MlsI TGGCCA 2 cut(s) 738, 1232
MluNI TGGCCA 2 cut(s) 738, 1232
MlyI GAGTC 8 cut(s) 451, 573, 735, 743, 1153, 1182, 1256, 1506
MmeI TCCRAC 2 cut(s) 274, 421
MnlI CCTC 9 cut(s) 276, 290, 592, 676, 853, 1249, 1318, 1757, 1793
Mox20I TGGCCA 2 cut(s) 738, 1232
MroI TCCGGA 1 cut(s) 711
MroXI GAANNNNTTC 3 cut(s) 70, 120, 474
MscI TGGCCA 2 cut(s) 738, 1232
MseI TTAA 4 cut(s) 450, 1182, 1698, 1761
Msp20I TGGCCA 2 cut(s) 738, 1232
MspI CCGG 2 cut(s) 712, 1389
MspR9I CCNGG 1 cut(s) 851
MunI CAATTG 2 cut(s) 50, 798
Mva1269I GAATGC 1 cut(s) 481
MvaI CCWGG 1 cut(s) 851
MvnI CGCG 1 cut(s) 18
NcoI CCATGG 2 cut(s) 792, 1272
NdeII GATC 4 cut(s) 19, 179, 973, 1690
NlaIV GGNNCC 2 cut(s) 181, 1098
NmuCI GTSAC 2 cut(s) 727, 764
NruI TCGCGA 1 cut(s) 18
NspV TTCGAA 1 cut(s) 220
PagI TCATGA 1 cut(s) 1147
PcsI WCGNNNNNNNCGW 1 cut(s) 94
PctI GAATGC 1 cut(s) 481
PdmI GAANNNNTTC 3 cut(s) 70, 120, 474
PfeI GAWTC 2 cut(s) 127, 1370
PflMI CCANNNNNTGG 2 cut(s) 176, 1363
PkrI GCNGC 8 cut(s) 535, 886, 916, 993, 1060, 1081, 1425, 1609
PleI GAGTC 8 cut(s) 451, 573, 734, 743, 1152, 1182, 1256, 1505
PpsI GAGTC 8 cut(s) 451, 573, 734, 743, 1152, 1182, 1256, 1505
Psp124BI GAGCTC 1 cut(s) 1339
Psp6I CCWGG 1 cut(s) 849
PspGI CCWGG 1 cut(s) 849
PspN4I GGNNCC 2 cut(s) 181, 1098
PspPI GGNCC 2 cut(s) 932, 1397
PstNI CAGNNNCTG 1 cut(s) 366
PsuI RGATCY 1 cut(s) 179
RruI TCGCGA 1 cut(s) 18
RsaI GTAC 1 cut(s) 265
RsaNI GTAC 1 cut(s) 264
SacI GAGCTC 1 cut(s) 1339
SaqAI TTAA 4 cut(s) 450, 1182, 1698, 1761
SatI GCNGC 8 cut(s) 534, 885, 915, 992, 1059, 1080, 1424, 1608
Sau3AI GATC 4 cut(s) 19, 179, 973, 1690
Sau96I GGNCC 2 cut(s) 932, 1397
ScaI AGTACT 1 cut(s) 265
SchI GAGTC 8 cut(s) 451, 573, 735, 743, 1153, 1182, 1256, 1506
ScrFI CCNGG 1 cut(s) 851
SduI GDGCHC 1 cut(s) 1339
SfaNI GCATC 5 cut(s) 592, 823, 1362, 1641, 1644
SfcI CTRYAG 2 cut(s) 232, 1004
SfuI TTCGAA 1 cut(s) 220
SinI GGWCC 1 cut(s) 1397
SmlI CTYRAG 5 cut(s) 287, 567, 981, 1667, 1718
SmoI CTYRAG 5 cut(s) 287, 567, 981, 1667, 1718
SsiI CCGC 3 cut(s) 5, 101, 467
SspMI CTAG 3 cut(s) 413, 776, 1427
SstI GAGCTC 1 cut(s) 1339
StyD4I CCNGG 1 cut(s) 849
StyI CCWWGG 5 cut(s) 640, 666, 792, 1083, 1272
TaaI ACNGT 4 cut(s) 527, 557, 910, 1492
TaiI ACGT 2 cut(s) 100, 420
TaqI TCGA 3 cut(s) 88, 220, 1500
TatI WGTACW 1 cut(s) 263
TfiI GAWTC 2 cut(s) 127, 1370
Tru1I TTAA 4 cut(s) 450, 1182, 1698, 1761
Tru9I TTAA 4 cut(s) 450, 1182, 1698, 1761
TscAI CASTG 3 cut(s) 1027, 1233, 1399
TseFI GTSAC 2 cut(s) 727, 764
TseI GCWGC 8 cut(s) 533, 884, 914, 991, 1058, 1079, 1423, 1607
Tsp45I GTSAC 2 cut(s) 727, 764
TspGWI ACGGA 1 cut(s) 150
TspRI CASTG 3 cut(s) 1027, 1233, 1399
Van91I CCANNNNNTGG 2 cut(s) 176, 1363
VpaK11BI GGWCC 1 cut(s) 1397
XapI RAATTY 4 cut(s) 42, 346, 628, 1559
XbaI TCTAGA 1 cut(s) 412
XcmI CCANNNNNNNNNTGG 1 cut(s) 517
XmiI GTMKAC 2 cut(s) 747, 1446
XmnI GAANNNNTTC 3 cut(s) 70, 120, 474
XspI CTAG 3 cut(s) 413, 776, 1427
ZrmI AGTACT 1 cut(s) 265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.