MD16G1139200.v1.1

calmodulin-binding transcription activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001204
Physical Location & Seq
Forward (+)
10720566 .. 10723554
2989 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1139200.v1.1.491

Sequence Viewer

Length: 1584 bp
ATGTTTGGTGAAATAGAAGTTTCTGCTGAAATTCTATCTAACAATGCCATTAGATGTCAAACTCCTTTGCATGCTCCTGGGCGTGTTCCATTCTATGTGACCGGCAGAAATAGGTTAGCCTGCAGTGAGATTAGGGAGTTTGAATACCGCAAAAAACCCTTTGGAATAGCTATCAACAACTTACAAGATGATGAATTGCACTTTCAGATACGACTAGCAAAATTGTTGAGCTTGGGCTCAAAGAGGAAGTGGTTGATATGTGCTGTTCCAGATTGTGACAAGTGTAAGCTCAAAAGTTCCATATTTTCAATGCAAGGCAACAGTGAAAGTGATTGGGTAACAATTGATGGGGCTTCTGTGTCATGCAAAAGTGATCACTTGAGCCGTAGGGATGTCTTGATTCAGAATTTGTTGAAGGATAGACTTTGTGAATGGCTAGTCTGTAAAATTCATGGAGGAGGCAAAGGACCACATGTTCTGGATAATGAAGGCCAAGGTGTTCTACATTTGACGGCTGCTCTTGGTTACAAGTGGGCCATAGGTCCCATAATTGCTGCAGGCGTCAGTCCCAATTTCAGAGATGCTCGCGGCAGAACGGGGCTTCACTGGGCATCATATTTTGGAAGAGGAAACTGTCATCACGTTACTTCGATTAGGTGCCACTCCAGGAGCCGTCGAGGACCCAACGTCGATATTTCAGGCGGACCAACTGCTGCTGATCTAGCCTCGAGTAGAGGGCATAAAGGGATTGCTGGATATTTGGCAGAAGCGGATTTAACAAGTCACCTAGAGTTGTTGACCATGAATGAGAACATAATGAGCAATGTTACCGCAACTATTGCAGCTGAGAACGCCAATCAGACTGCAGAAGTTCCTGCATCTGCTCCAGTAGATGAGCAATATTCTCTCAAAAACCCTATAGCAGCTGTTAGGAAATCAGCTCATGCTGCTGCTCTAATTCAAGAAGCTTTCCAAGCTCGTTCATTCCGTCAGAGACAGTTAACCAAGAGTAGAACAGATGTTTCTGAAGTTCGATCTGAGGACCTTGTTGCACGTCATTCCTTGAAGAGAGTCCAGAAGTTCGCTCACTATGAAGATTATCTGCATGTCGCAGCAGCTCTGAAGATTCAACAAAACTATCGTGGATGGAAGGGAAGAAAGGATTATCTGAAGATTCGTGACCGTATTGTTAAAATTCAGGCTCATGTGAGGGGACATCAGGTCCGGAAGAATTACAAAAAGGTTTTAAGAACTGGTCTGCGAGGATTTCGGGTGGAAAAGCCTACTGAAGATGCATCCTCAGAGGTTAAAAAATATGATGACTATGAATTTCTTAGTGTCAGTCGGAAGCAGAAATTTGCTGGGGTTGAGAAAGCTCTTGCAAGGGTACAGTTCATGGCTCGTCAGCCTGAAGCTCGTGAACAGTATATGAGGCTAATTTCAAACTTTGAGAAACTTGAGATGACTGACGGGAAGAACCAGGCCTCTTATCAAAATGAAAGTTCCAACCAACAAGTCATAGATGAAATTCTGCGTTCACTCAATGAAGGTCAACGATCAACTTCCACCATGTCAAGTAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000981 GO:0000982 GO:0001077 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009409 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010150 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031349 GO:0032501 GO:0032502 GO:0032774 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070417 GO:0071704 GO:0080090 GO:0080134 GO:0090304 GO:0090693 GO:0097159 GO:0097659 GO:0098542 GO:0099402 GO:0140110 GO:1900367 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000068 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 CG-1 IQ TIG

Protein Analysis

528

Amino Acids

59.19

Weight (kDa)

9.55

Isoelectric Point (pI)

36.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 657
AccII CGCG 1 cut(s) 588
AccIII TCCGGA 1 cut(s) 1224
AciI CCGC 5 cut(s) 148, 588, 702, 770, 831
AcsI RAATTY 7 cut(s) 30, 406, 447, 1194, 1328, 1355, 1527
AcuI CTGAAG 5 cut(s) 1047, 1142, 1190, 1308, 1431
AcyI GRCGYC 1 cut(s) 561
AfaI GTAC 1 cut(s) 1389
AflIII ACRYGT 1 cut(s) 472
AgsI TTSAA 7 cut(s) 143, 309, 415, 962, 1066, 1130, 1443
AhdI GACNNNNNGTC 2 cut(s) 686, 1220
AjiI CACGTC 1 cut(s) 1055
AjnI CCWGG 3 cut(s) 76, 665, 1479
AloI GAACNNNNNNTCC 2 cut(s) 459, 491
Alw26I GTCTC 1 cut(s) 988
Ama87I CYCGRG 1 cut(s) 727
Aor13HI TCCGGA 1 cut(s) 1224
AoxI GGCC 3 cut(s) 490, 534, 1482
ApeKI GCWGC 9 cut(s) 515, 554, 713, 842, 923, 947, 950, 1112, 1115
ApoI RAATTY 7 cut(s) 30, 406, 447, 1194, 1328, 1355, 1527
AspS9I GGNCC 7 cut(s) 467, 534, 542, 680, 704, 1042, 1222
AsuHPI GGTGA 2 cut(s) 20, 776
AvaI CYCGRG 1 cut(s) 727
AvaII GGWCC 6 cut(s) 467, 542, 680, 704, 1042, 1222
BanI GGYRCC 1 cut(s) 657
BanII GRGCYC 1 cut(s) 239
BauI CACGAG 1 cut(s) 1416
BbvI GCAGC 9 cut(s) 502, 541, 700, 854, 934, 935, 937, 1124, 1127
BccI CCATC 2 cut(s) 341, 1140
BceAI ACGGC 3 cut(s) 369, 528, 657
BciT130I CCWGG 3 cut(s) 78, 667, 1481
BclI TGATCA 1 cut(s) 373
BcoDI GTCTC 1 cut(s) 988
BfaI CTAG 4 cut(s) 215, 437, 722, 788
BfmI CTRYAG 4 cut(s) 121, 555, 864, 918
Bme1390I CCNGG 3 cut(s) 78, 667, 1481
Bme18I GGWCC 6 cut(s) 467, 542, 680, 704, 1042, 1222
BmeRI GACNNNNNGTC 2 cut(s) 686, 1220
BmeT110I CYCGRG 1 cut(s) 727
BmgBI CACGTC 1 cut(s) 1055
BmgT120I GGNCC 7 cut(s) 467, 534, 542, 680, 704, 1042, 1222
BmiI GGNNCC 4 cut(s) 544, 659, 671, 682
BmrFI CCNGG 3 cut(s) 78, 667, 1481
BmrI ACTGGG 1 cut(s) 616
BmsI GCATC 5 cut(s) 571, 620, 887, 1282, 1304
BmuI ACTGGG 1 cut(s) 616
BpmI CTGGAG 2 cut(s) 649, 870
BpuEI CTTGAG 2 cut(s) 400, 1478
BsaHI GRCGYC 1 cut(s) 561
BsaJI CCNNGG 2 cut(s) 77, 493
BsaWI WCCGGW 1 cut(s) 1224
Bse118I RCCGGY 1 cut(s) 101
Bse1I ACTGG 3 cut(s) 611, 887, 1258
Bse3DI GCAATG 1 cut(s) 829
BseAI TCCGGA 1 cut(s) 1224
BseBI CCWGG 3 cut(s) 78, 667, 1481
BseDI CCNNGG 2 cut(s) 77, 493
BseGI GGATG 3 cut(s) 397, 1151, 1295
BseMI GCAATG 1 cut(s) 829
BseMII CTCAG 3 cut(s) 837, 1029, 1314
BseNI ACTGG 3 cut(s) 611, 887, 1258
BseRI GAGGAG 1 cut(s) 471
BseXI GCAGC 9 cut(s) 502, 541, 700, 854, 934, 935, 937, 1124, 1127
BseYI CCCAGC 1 cut(s) 1361
Bsh1236I CGCG 1 cut(s) 588
BshFI GGCC 3 cut(s) 492, 536, 1484
BshNI GGYRCC 1 cut(s) 657
BsiHKCI CYCGRG 1 cut(s) 727
BsiSI CCGG 2 cut(s) 102, 1225
BslFI GGGAC 3 cut(s) 528, 552, 1227
BsmAI GTCTC 1 cut(s) 988
BsmFI GGGAC 3 cut(s) 528, 552, 1227
BsnI GGCC 3 cut(s) 492, 536, 1484
BsoBI CYCGRG 1 cut(s) 727
Bsp1286I GDGCHC 1 cut(s) 239
Bsp13I TCCGGA 1 cut(s) 1224
Bsp143I GATC 4 cut(s) 373, 718, 1034, 1556
BspACI CCGC 5 cut(s) 148, 588, 702, 770, 831
BspANI GGCC 3 cut(s) 492, 536, 1484
BspCNI CTCAG 3 cut(s) 838, 1030, 1313
BspEI TCCGGA 1 cut(s) 1224
BspFNI CGCG 1 cut(s) 588
BspLI GGNNCC 4 cut(s) 544, 659, 671, 682
BspMAI CTGCAG 3 cut(s) 125, 559, 868
BspT107I GGYRCC 1 cut(s) 657
BsrDI GCAATG 1 cut(s) 829
BsrFI RCCGGY 1 cut(s) 101
BsrI ACTGG 3 cut(s) 611, 887, 1258
BssAI RCCGGY 1 cut(s) 101
BssECI CCNNGG 2 cut(s) 77, 493
BssMI GATC 4 cut(s) 373, 718, 1034, 1556
BssNI GRCGYC 1 cut(s) 561
BssSI CACGAG 1 cut(s) 1416
BssT1I CCWWGG 1 cut(s) 493
Bst2BI CACGAG 1 cut(s) 1416
Bst2UI CCWGG 3 cut(s) 78, 667, 1481
Bst4CI ACNGT 6 cut(s) 323, 635, 999, 1184, 1392, 1425
Bst6I CTCTTC 2 cut(s) 619, 1061
BstACI GRCGYC 1 cut(s) 561
BstAPI GCANNNNNTGC 1 cut(s) 839
BstC8I GCNNGC 4 cut(s) 72, 121, 559, 586
BstDEI CTNAG 4 cut(s) 846, 1038, 1300, 1334
BstF5I GGATG 3 cut(s) 397, 1151, 1295
BstFNI CGCG 1 cut(s) 588
BstKTI GATC 4 cut(s) 376, 721, 1037, 1559
BstMAI GTCTC 1 cut(s) 988
BstMBI GATC 4 cut(s) 373, 718, 1034, 1556
BstMWI GCNNNNNNNGC 5 cut(s) 722, 839, 851, 947, 974
BstNI CCWGG 3 cut(s) 78, 667, 1481
BstNSI RCATGY 3 cut(s) 74, 476, 1109
BstSCI CCNGG 3 cut(s) 76, 665, 1479
BstSFI CTRYAG 4 cut(s) 121, 555, 864, 918
BstUI CGCG 1 cut(s) 588
BstV1I GCAGC 9 cut(s) 502, 541, 700, 854, 934, 935, 937, 1124, 1127
BsuRI GGCC 3 cut(s) 492, 536, 1484
BtrI CACGTC 1 cut(s) 1055
BtsCI GGATG 3 cut(s) 397, 1151, 1295
BtsI GCAGTG 1 cut(s) 130
BtsIMutI CAGTG 3 cut(s) 130, 328, 604
Cac8I GCNNGC 4 cut(s) 72, 121, 559, 586
Cfr10I RCCGGY 1 cut(s) 101
Cfr13I GGNCC 7 cut(s) 467, 534, 542, 680, 704, 1042, 1222
CseI GACGC 1 cut(s) 550
Csp6I GTAC 1 cut(s) 1388
CviQI GTAC 1 cut(s) 1388
DdeI CTNAG 4 cut(s) 846, 1038, 1300, 1334
DpnI GATC 4 cut(s) 375, 720, 1036, 1558
DpnII GATC 4 cut(s) 373, 718, 1034, 1556
DriI GACNNNNNGTC 2 cut(s) 686, 1220
Eam1104I CTCTTC 2 cut(s) 619, 1061
Eam1105I GACNNNNNGTC 2 cut(s) 686, 1220
EarI CTCTTC 2 cut(s) 619, 1061
EciI GGCGGA 1 cut(s) 717
Eco130I CCWWGG 1 cut(s) 493
Eco147I AGGCCT 1 cut(s) 1484
Eco24I GRGCYC 1 cut(s) 239
Eco47I GGWCC 6 cut(s) 467, 542, 680, 704, 1042, 1222
Eco57I CTGAAG 5 cut(s) 1047, 1142, 1190, 1308, 1431
Eco88I CYCGRG 1 cut(s) 727
EcoO109I RGGNCCY 3 cut(s) 542, 680, 1042
EcoRII CCWGG 3 cut(s) 76, 665, 1479
EcoT14I CCWWGG 1 cut(s) 493
EcoT22I ATGCAT 1 cut(s) 1297
EcoT38I GRGCYC 1 cut(s) 239
ErhI CCWWGG 1 cut(s) 493
FaqI GGGAC 3 cut(s) 528, 552, 1227
FbaI TGATCA 1 cut(s) 373
FokI GGATG 3 cut(s) 404, 1158, 1282
FriOI GRGCYC 1 cut(s) 239
FspBI CTAG 4 cut(s) 215, 437, 722, 788
GsaI CCCAGC 1 cut(s) 1365
GsuI CTGGAG 2 cut(s) 649, 870
HaeIII GGCC 3 cut(s) 492, 536, 1484
HapII CCGG 2 cut(s) 102, 1225
HgaI GACGC 1 cut(s) 550
Hin1I GRCGYC 1 cut(s) 561
HincII GTYRAC 3 cut(s) 798, 1002, 1553
HindII GTYRAC 3 cut(s) 798, 1002, 1553
HindIII AAGCTT 1 cut(s) 966
HinfI GANTC 4 cut(s) 400, 1071, 1126, 1174
HpaI GTTAAC 1 cut(s) 1002
HpaII CCGG 2 cut(s) 102, 1225
HphI GGTGA 2 cut(s) 20, 776
Hpy166II GTNNAC 5 cut(s) 798, 1002, 1421, 1538, 1553
Hpy188III TCNNGA 8 cut(s) 269, 397, 479, 962, 1075, 1178, 1225, 1418
Hpy8I GTNNAC 5 cut(s) 798, 1002, 1421, 1538, 1553
Hpy99I CGWCG 2 cut(s) 678, 692
HpyAV CCTTC 4 cut(s) 409, 482, 1144, 1541
HpyCH4III ACNGT 6 cut(s) 323, 635, 999, 1184, 1392, 1425
HpyCH4IV ACGT 3 cut(s) 642, 687, 1054
HpyF10VI GCNNNNNNNGC 5 cut(s) 722, 839, 851, 947, 974
HpyF3I CTNAG 4 cut(s) 846, 1038, 1300, 1334
HpySE526I ACGT 3 cut(s) 642, 687, 1054
Hsp92I GRCGYC 1 cut(s) 561
Kpn2I TCCGGA 1 cut(s) 1224
Ksp22I TGATCA 1 cut(s) 373
KspAI GTTAAC 1 cut(s) 1002
Kzo9I GATC 4 cut(s) 373, 718, 1034, 1556
LmnI GCTCC 3 cut(s) 79, 669, 889
Lsp1109I GCAGC 9 cut(s) 502, 541, 700, 854, 934, 935, 937, 1124, 1127
LweI GCATC 5 cut(s) 571, 620, 887, 1282, 1304
MaeI CTAG 4 cut(s) 215, 437, 722, 788
MaeII ACGT 3 cut(s) 642, 687, 1054
MaeIII GTNAC 9 cut(s) 97, 275, 337, 524, 643, 782, 826, 1178, 1577
MalI GATC 4 cut(s) 375, 720, 1036, 1558
MboI GATC 4 cut(s) 373, 718, 1034, 1556
MboII GAAGA 9 cut(s) 636, 1078, 1106, 1135, 1167, 1183, 1240, 1301, 1486
MfeI CAATTG 1 cut(s) 342
MhlI GDGCHC 1 cut(s) 239
MlyI GAGTC 1 cut(s) 1080
MmeI TCCRAC 2 cut(s) 1325, 1530
Mph1103I ATGCAT 1 cut(s) 1297
MroI TCCGGA 1 cut(s) 1224
MseI TTAA 5 cut(s) 776, 1001, 1191, 1247, 1308
MspA1I CMGCKG 2 cut(s) 845, 926
MspI CCGG 2 cut(s) 102, 1225
MspR9I CCNGG 3 cut(s) 78, 667, 1481
MunI CAATTG 1 cut(s) 342
MvaI CCWGG 3 cut(s) 78, 667, 1481
MvnI CGCG 1 cut(s) 588
MwoI GCNNNNNNNGC 5 cut(s) 722, 839, 851, 947, 974
NdeII GATC 4 cut(s) 373, 718, 1034, 1556
NlaIV GGNNCC 4 cut(s) 544, 659, 671, 682
NmuCI GTSAC 4 cut(s) 97, 275, 782, 1178
NsiI ATGCAT 1 cut(s) 1297
NspI RCATGY 3 cut(s) 74, 476, 1109
PaeI GCATGC 1 cut(s) 74
PaeR7I CTCGAG 1 cut(s) 727
PceI AGGCCT 1 cut(s) 1484
PciI ACATGT 1 cut(s) 472
PcsI WCGNNNNNNNCGW 1 cut(s) 985
PfeI GAWTC 3 cut(s) 400, 1126, 1174
PfoI TCCNGGA 1 cut(s) 665
PleI GAGTC 1 cut(s) 1079
PpsI GAGTC 1 cut(s) 1079
PpuMI RGGWCCY 3 cut(s) 542, 680, 1042
PscI ACATGT 1 cut(s) 472
Psp5II RGGWCCY 3 cut(s) 542, 680, 1042
Psp6I CCWGG 3 cut(s) 76, 665, 1479
PspFI CCCAGC 1 cut(s) 1361
PspGI CCWGG 3 cut(s) 76, 665, 1479
PspN4I GGNNCC 4 cut(s) 544, 659, 671, 682
PspPI GGNCC 7 cut(s) 467, 534, 542, 680, 704, 1042, 1222
PspPPI RGGWCCY 3 cut(s) 542, 680, 1042
PspXI VCTCGAGB 1 cut(s) 727
PstI CTGCAG 3 cut(s) 125, 559, 868
PvuII CAGCTG 2 cut(s) 845, 926
RsaI GTAC 1 cut(s) 1389
RsaNI GTAC 1 cut(s) 1388
SaqAI TTAA 5 cut(s) 776, 1001, 1191, 1247, 1308
Sau3AI GATC 4 cut(s) 373, 718, 1034, 1556
Sau96I GGNCC 7 cut(s) 467, 534, 542, 680, 704, 1042, 1222
SchI GAGTC 1 cut(s) 1080
ScrFI CCNGG 3 cut(s) 78, 667, 1481
SduI GDGCHC 1 cut(s) 239
SfaNI GCATC 5 cut(s) 571, 620, 887, 1282, 1304
SfcI CTRYAG 4 cut(s) 121, 555, 864, 918
Sfr274I CTCGAG 1 cut(s) 727
SinI GGWCC 6 cut(s) 467, 542, 680, 704, 1042, 1222
SlaI CTCGAG 1 cut(s) 727
SmlI CTYRAG 3 cut(s) 379, 727, 1457
SmoI CTYRAG 3 cut(s) 379, 727, 1457
SphI GCATGC 1 cut(s) 74
SseBI AGGCCT 1 cut(s) 1484
SsiI CCGC 5 cut(s) 148, 588, 702, 770, 831
SspI AATATT 1 cut(s) 902
SspMI CTAG 4 cut(s) 215, 437, 722, 788
StuI AGGCCT 1 cut(s) 1484
StyD4I CCNGG 3 cut(s) 76, 665, 1479
StyI CCWWGG 1 cut(s) 493
TaaI ACNGT 6 cut(s) 323, 635, 999, 1184, 1392, 1425
TaiI ACGT 3 cut(s) 645, 690, 1057
TaqI TCGA 5 cut(s) 650, 676, 690, 728, 1033
TauI GCSGC 1 cut(s) 591
TfiI GAWTC 3 cut(s) 400, 1126, 1174
Tru1I TTAA 5 cut(s) 776, 1001, 1191, 1247, 1308
Tru9I TTAA 5 cut(s) 776, 1001, 1191, 1247, 1308
TscAI CASTG 3 cut(s) 130, 328, 611
TseFI GTSAC 4 cut(s) 97, 275, 782, 1178
TseI GCWGC 9 cut(s) 515, 554, 713, 842, 923, 947, 950, 1112, 1115
Tsp45I GTSAC 4 cut(s) 97, 275, 782, 1178
TspGWI ACGGA 1 cut(s) 977
TspRI CASTG 3 cut(s) 130, 328, 611
VpaK11BI GGWCC 6 cut(s) 467, 542, 680, 704, 1042, 1222
XapI RAATTY 7 cut(s) 30, 406, 447, 1194, 1328, 1355, 1527
XceI RCATGY 3 cut(s) 74, 476, 1109
XhoI CTCGAG 1 cut(s) 727
XspI CTAG 4 cut(s) 215, 437, 722, 788
Zsp2I ATGCAT 1 cut(s) 1297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.