Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0001204

Physical Location & Seq

Genomic Coordinates Reverse (-)

7632862 .. 7640895

Gene Structure 8034 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1044600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3213 bp

ATGTCGGAAAGCGGATCGTACCCTCAGGGCCCTCGATTAGACTTTCAGCAATTACTTGTTGAAGCCCAACACCGATGGCTGCGGCCTGCTGAAATCTGTGAAATTCTTAGCAATTTTGAGAAGTTCCAAATCTCATCAGAGCCTCCGAACAAACCACCTAGCGGTTCGCTCTATCTTTTTGACAGGAAGGTTCTGAGATACTTCAGAAAGGATGGACATAACTGGAGGAAGAAACGAGATGGCAAGACTGTGAAGGAGGCCCATGAGAAGCTAAAGGTTGGCAGTGTTGATGTTCTACACTGCTACTATGCCCATGGAGAAGATGATGAAAGTTTTCAAAGGCGCTGCTATTGGATGCTTGAGCAGGATTTAATGCACATAGTTTTTGTTCATTACTTGCAAGTAAAGGGTAATAGGGCAAATGCAGGAGGCATAAGAGAGAATGATGAGGTTGCAGCGGATCTGCAAAAGGGCAGCCCCTGGACTTCTAGCCCTTCTAATAGCAATGGCAGAGCACCTTCAGGATATACAGATTATGCAAGCCCAAGCAGCACCCTTACATCATCATGGGAAGATGTTGATTCCGGAGATAGTCGCCAAGCAAGTTCCTTATTTCACTCAGTTTTTGAGTCACCAAAAATGGGGAATGGTCCTCTCGTGGACAATGCGGAGATTGATCCCTCACTGCATTCAAGTTTAAAAAATCATGATGGCCAGTCATCAATTCATGGAGTATACAGGCCAGAATTTGACAAAGATCAACATTATTTCACTGATGACTCTACTTGTGTGATTGATTCTCAAAAAACACTTGGTGTGGGATCTTGGGAAGAAATATTGGAACAATGCACAATGGGATTACATGATGGAACTTCTCATGCATCAATGTCTTCCACCCAAATTAGTTCTGCTGGAGTTCCCCTTGAACAACAAACTTCTGGGAATAACCTGTTAAAGGAGGAGTTTGGGAGTTCTCTGCCATTGCAAACAAATTGGCAGTTTCCTTTGGGAGACAATTCACTGCAGTTGCCTGAGTGGTCCCTGGATCAGTCGGTGAATTTGCAAAGAACTCTTGATGCGAATCTGAGCAATGCTCCAGACCTTGTTGGTGCTCATCTCAATCAGCGAAATGAGCAGCTTGTGCAGGACAACCTTCAAGCACAGCTTACAAATGCAGAATCTCAAAGTTTAATCATATCAAGTTCTGAGCATGATGTTCCGAAAGATGGGAATATCAACTATGCTTTCACTTTGAGACAGCAATTATTAGATCGAGAAGAGGGTTTGAAGAAAGTTGACAGCTTTTCTAGGTGGGTTTCCAAGGAACTTGGAGAGGTAGATGATCTACAGATGCAATCCTCATCTGGTATTTCATGGATCACTGCTGAATGTGGAGATGTAGTTGATGATTCCTCGTTGAGCCCCTCTATCTCCCAGGACCAGCTTTTCAGCATAGTTGATTTTTCACCAAAGTGGGCATACACAGACTCTGAAATTGAGATTCTGGTTTTTGGAACATTCTTGGTAAGTCAAAAGGAGGTAACAAAATACAGCTGGTCTTGTATGTTTGGGGAGGTGGAAGTTCCAGCAAAGGTTTTAGCCAGTGGAGTTCTTTTCTGTTTTGCTCCACCTCACAGTGCCGGGCAAGTCCCCTTCTATGTAACTTGTTCCAACAGGTTAGCTTGTAGTGAAGTAAGAGAGTTCGATTATCAAGTGGGCTCCACCAAAGGTTTAGATATGACAGATATATGCAATGACACTGCACACGAAATGCATCTTCACTTACGTCTTGAGAGTTTACTGTCTCTGAGGTCTGTCAGCCCTTCAGGTCACCTGTTTGGAGGTGTTAAGGAGAATCGAAACATAATTAGTAAAATCATATCACTGAAGGAGGAAGAGGATTATTTACATGCAGCAGAACCAACAGTAGCGAATTATTTGCTCCAAAATGAAGGGATGGAACATCTTATAAAGCTGATGAAAGAGAAGTTGTACACTTGGCTACTTTATAAAGCAATAGAAGATGGTAAAGGACCAAGTGTATTAGATGCTGAAGGACAAGGTGTGCTACATTTAGCAGCTGCCCTTTCCTACAATTGGGCCATAAAACCCACTGTAACTGCGGGAGTTAGTATCAATTTTCGTGATGTTAATGGATGGACCGCCCTTCATTGGGCTGCATTCTATGGCAGAGAGCAGACAGTTGCAGTCCTTGTCTCTCTTGGTGCCGATCCTGGAGCACTGACCGACCCGTCTCCAGAGGTACCTTTGGGTAGAACACCAGCAGACCTGGCATCTGTGAACAGTCACAAAGGAATTTCAGGCTTCCTCGCAGAGTCTTCCCTGACCACTTTCCTTAAATCCCTTACAATGAATGATGCAAAGGAAGATAGTGCTGCAGAAATATCTGGAACAAAAGTTGTCAAAACTTTTTCAGAACGGATAGCTACACCTGTGAGTTACGGTGATATGCCCGATGCGCTGTCACTGAAGGACTCACTTACTGCTGTAACTAATGCTACCCAAGCTGCTGATCGTATACAGCAAATGTTTAGGATGCTGTCATTTGAGAGGAGACGAATTACTGAGCATGACATTGATGAGTTCGGAATGCCGGATGAGAGAGCAATTTCACTCATAACTTCCAAATCACACAAGGTGGGACCGGTCAAAGGGTTTGCTCATACTGCTGCAGTTCAGATACAGAAAAAGTTTCGTGGTTGGAAGAAAAGAAAAGAATTCCTGATAATTCGCCAGAGAATTGTTAAAATCCAGGCCCATGTAAGGGGACACCAGGTAAGGAAACAATATAAGGCAATCACCTGGTCAGTTGGAATTCTGGAGAAAGTTATTTTGCGTTGGAGACGTAAGGGGACTGGTTTACGAGGATTCCGACCGGATGCAGTTGCCAAGATTCCGAGCCCACAACCTGTGCCCTCAAACGAGGATGATTATGACTTTCTTAAGAAAGGAAGAAAACAAACAGAGGAAAGGTTACAAAAAGCACTCACTAGGGTAAAGTCTATGGTTCAGTATCCAGAGGGAAGAGCTCAATACCGTAGACTGCTAAATGTTGTCGAAGGATTCCGTGAAACAAAGGTAAGTGGTATGGCTGTCGAGGGATCAGAAGAAAAGGCCGAGGGTGGTGAGGATCTAATCGATATTGACAAGTTGTTGGATGATGACACTTTCATGTCTATAGCATTTGATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000981 GO:0000982 GO:0001077 GO:0001101 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009409 GO:0009414 GO:0009415 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010044 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050826 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051716 GO:0060255 GO:0065007 GO:0070887 GO:0071241 GO:0071248 GO:0071275 GO:0071704 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1901700 GO:1902680 GO:1903506 GO:1903508 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Ank_2 CG-1 IQ TIG

Protein Analysis

1071

Amino Acids

119.44

Weight (kDa)

5.59

Isoelectric Point (pI)

48.68

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CG-1	PF03859	21 - 134	4.3e-51	CG-1 domain
Ank_2	PF12796	673 - 746	2.6e-07	Ankyrin repeats (3 copies)
Ank_2	PF12796	690 - 779	3.3e-07	Ankyrin repeats (3 copies)
Ank	PF00023	718 - 749	4.3e-06	Ankyrin repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001204)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G09410 AT5G09410 AT5G09410 AT5G09410 AT5G64220 AT5G64220
fragaria_vesca	FvH4_5g27670 FvH4_5g27670 FvH4_5g27670 FvH4_5g27670
malus_domestica	MD05G1044600.v1.1 MD10G1051700.v1.1 MD12G1040100.v1.1 MD13G1144300.v1.1 MD16G1084000.v1.1 MD16G1139000.v1.1 MD16G1139200.v1.1
prunus_persica	Prupe.1G108700_v2.0.a1 Prupe.1G108700_v2.0.a1 Prupe.1G108700_v2.0.a1 Prupe.1G108700_v2.0.a1 Prupe.1G108700_v2.0.a1 Prupe.8G060300_v2.0.a1
pyrus_communis	pycom01g00940 pycom05g03720 pycom10g03650 pycom12g03420 pycom13g12490 pycom16g12350 pycom16g12360
rosa_chinensis	RchiOBHm_Chr2g0113081 RchiOBHm_Chr7g0219321
rosa_laevigata	RLG00000002297 RLG00000022767
rosa_multiflora	Rmu_sc0029995.1_g000002
rosa_roxburghii	Rroxscaffold_2G00129370 Rroxscaffold_3G00240090
rosa_rugosa	Rorug01G0154600.1 Rorug07G0184300
rosa_samantha	Rh2AG238800 Rh2AG238900 Rh2AG324900 Rh2BG252600 Rh2CG242800 Rh2CG242900 Rh2DG246800 Rh4BG352900 Rh5AG518400 Rh7AG327200 Rh7BG317200 Rh7CG344500 Rh7DG209600 Rh7DG322800
rosa_wichuraiana	Rw0G004510 Rw2G018730 Rw7G027570

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1970, 2010
AasI	GACNNNNNNGTC	1 cut(s)	2251
Acc65I	GGTACC	1 cut(s)	2263
AccB1I	GGYRCC	2 cut(s)	2225, 2263
AccI	GTMKAC	3 cut(s)	735, 2539, 3061
AccIII	TCCGGA	1 cut(s)	584
AciI	CCGC	7 cut(s)	12, 82, 162, 458, 668, 2123, 2163
AclWI	GGATC	9 cut(s)	22, 468, 671, 829, 1053, 1385, 2225, 3130, 3159
AcoI	YGGCCR	1 cut(s)	712
AcsI	RAATTY	6 cut(s)	102, 746, 1057, 2316, 2738, 2835
AcuI	CTGAAG	6 cut(s)	187, 504, 1809, 1907, 2073, 2510
AdeI	CACNNNGTG	2 cut(s)	815, 2659
AfaI	GTAC	3 cut(s)	20, 1994, 2265
AflII	CTTAAG	1 cut(s)	2963
AgeI	ACCGGT	1 cut(s)	2665
AgsI	TTSAA	6 cut(s)	62, 338, 693, 926, 1157, 1288
AjnI	CCWGG	8 cut(s)	479, 1041, 1434, 2233, 2289, 2772, 2793, 2822
AleI	CACNNNNGTG	1 cut(s)	1471
Alw21I	GWGCWC	4 cut(s)	517, 1114, 2242, 3052
Alw26I	GTCTC	7 cut(s)	1005, 1249, 1809, 2221, 2259, 2569, 2857
AlwI	GGATC	9 cut(s)	22, 468, 671, 829, 1053, 1385, 2225, 3130, 3159
AlwNI	CAGNNNCTG	2 cut(s)	480, 1490
Aor13HI	TCCGGA	1 cut(s)	584
AoxI	GGCC	8 cut(s)	28, 83, 258, 712, 740, 2100, 2775, 3135
ApaI	GGGCCC	1 cut(s)	32
ApoI	RAATTY	6 cut(s)	102, 746, 1057, 2316, 2738, 2835
AsiGI	ACCGGT	1 cut(s)	2665
Asp700I	GAANNNNTTC	1 cut(s)	333
Asp718I	GGTACC	1 cut(s)	2263
AspLEI	GCGC	2 cut(s)	345, 2482
AsuC2I	CCSGG	1 cut(s)	1642
AsuHPI	GGTGA	7 cut(s)	624, 1066, 1458, 1823, 2477, 2812, 3158
AvaII	GGWCC	6 cut(s)	650, 1038, 1438, 2033, 2160, 2663
AxyI	CCTNAGG	1 cut(s)	24
BaeGI	GKGCMC	2 cut(s)	32, 2937
BalI	TGGCCA	1 cut(s)	714
BanI	GGYRCC	2 cut(s)	2225, 2263
BanII	GRGCYC	5 cut(s)	32, 1424, 1721, 2924, 3052
BauI	CACGAG	1 cut(s)	656
BbsI	GAAGAC	2 cut(s)	882, 2331
Bbv12I	GWGCWC	4 cut(s)	517, 1114, 2242, 3052
BccI	CCATC	9 cut(s)	69, 206, 233, 704, 860, 1220, 1951, 2018, 2151
BcgI	CGANNNNNNTGC	2 cut(s)	1921, 1955
BciT130I	CCWGG	8 cut(s)	481, 1043, 1436, 2235, 2291, 2774, 2795, 2824
BciVI	GTATCC	1 cut(s)	3045
BcnI	CCSGG	1 cut(s)	1642
BcoDI	GTCTC	7 cut(s)	1005, 1249, 1809, 2221, 2259, 2569, 2857
BfaI	CTAG	4 cut(s)	159, 489, 1308, 3012
BfmI	CTRYAG	5 cut(s)	1022, 1346, 2397, 2691, 3198
BfoI	RGCGCY	1 cut(s)	346
BfrI	CTTAAG	1 cut(s)	2963
BfuI	GTATCC	1 cut(s)	3045
Bme1390I	CCNGG	9 cut(s)	481, 1043, 1436, 1642, 2235, 2291, 2774, 2795, 2824
Bme18I	GGWCC	6 cut(s)	650, 1038, 1438, 2033, 2160, 2663
BmiI	GGNNCC	6 cut(s)	30, 1040, 1720, 2227, 2265, 2664
BmrFI	CCNGG	9 cut(s)	481, 1043, 1436, 1642, 2235, 2291, 2774, 2795, 2824
BpiI	GAAGAC	2 cut(s)	882, 2331
BplI	GAGNNNNNCTC	2 cut(s)	1078, 1110
BpmI	CTGGAG	6 cut(s)	244, 933, 1080, 2241, 2256, 2861
BpuEI	CTTGAG	2 cut(s)	380, 1811
BpuMI	CCSGG	1 cut(s)	1642
Bsa29I	ATCGAT	1 cut(s)	3159
BsaBI	GATNNNNATC	1 cut(s)	1080
BsaJI	CCNNGG	6 cut(s)	313, 479, 1041, 1320, 1434, 3138
BsaWI	WCCGGW	3 cut(s)	584, 2665, 2896
BsaXI	ACNNNNNCTCC	2 cut(s)	1386, 1416
Bse118I	RCCGGY	1 cut(s)	2665
Bse1I	ACTGG	4 cut(s)	227, 715, 1602, 2881
Bse21I	CCTNAGG	1 cut(s)	24
Bse3DI	GCAATG	4 cut(s)	511, 980, 1096, 1759
Bse8I	GATNNNNATC	1 cut(s)	1080
BseAI	TCCGGA	1 cut(s)	584
BseBI	CCWGG	8 cut(s)	481, 1043, 1436, 2235, 2291, 2774, 2795, 2824
BseCI	ATCGAT	1 cut(s)	3159
BseDI	CCNNGG	6 cut(s)	313, 479, 1041, 1320, 1434, 3138
BseGI	GGATG	9 cut(s)	217, 360, 1962, 2162, 2562, 2623, 2905, 2953, 3184
BseJI	GATNNNNATC	1 cut(s)	1080
BseMI	GCAATG	4 cut(s)	511, 980, 1096, 1759
BseMII	CTCAG	8 cut(s)	38, 185, 633, 1023, 1076, 1197, 1799, 2577
BseNI	ACTGG	4 cut(s)	227, 715, 1602, 2881
BseRI	GAGGAG	2 cut(s)	974, 2587
BseSI	GKGCMC	2 cut(s)	32, 2937
BsgI	GTGCAG	2 cut(s)	1163, 1746
Bsh1285I	CGRYCG	1 cut(s)	2897
BshFI	GGCC	8 cut(s)	30, 85, 260, 714, 742, 2102, 2777, 3137
BshNI	GGYRCC	2 cut(s)	2225, 2263
BshTI	ACCGGT	1 cut(s)	2665
BshVI	ATCGAT	1 cut(s)	3159
BsiEI	CGRYCG	1 cut(s)	2897
BsiHKAI	GWGCWC	4 cut(s)	517, 1114, 2242, 3052
BsiSI	CCGG	5 cut(s)	585, 1641, 2615, 2666, 2897
BslFI	GGGAC	5 cut(s)	1024, 1634, 2676, 2802, 2887
BsmAI	GTCTC	7 cut(s)	1005, 1249, 1809, 2221, 2259, 2569, 2857
BsmBI	CGTCTC	3 cut(s)	2259, 2569, 2857
BsmFI	GGGAC	5 cut(s)	1024, 1634, 2676, 2802, 2887
BsmI	GAATGC	3 cut(s)	688, 2180, 2616
BsnI	GGCC	8 cut(s)	30, 85, 260, 714, 742, 2102, 2777, 3137
Bsp120I	GGGCCC	1 cut(s)	28
Bsp1286I	GDGCHC	9 cut(s)	32, 517, 1114, 1424, 1721, 2242, 2924, 2937, 3052
Bsp13I	TCCGGA	1 cut(s)	584
Bsp1407I	TGTACA	1 cut(s)	1992
Bsp19I	CCATGG	1 cut(s)	313
BspACI	CCGC	7 cut(s)	12, 82, 162, 458, 668, 2123, 2163
BspANI	GGCC	8 cut(s)	30, 85, 260, 714, 742, 2102, 2777, 3137
BspCNI	CTCAG	8 cut(s)	37, 186, 632, 1024, 1077, 1198, 1800, 2578
BspDI	ATCGAT	1 cut(s)	3159
BspEI	TCCGGA	1 cut(s)	584
BspHI	TCATGA	1 cut(s)	706
BspLI	GGNNCC	6 cut(s)	30, 1040, 1720, 2227, 2265, 2664
BspMAI	CTGCAG	3 cut(s)	1026, 2401, 2695
BspPI	GGATC	9 cut(s)	22, 468, 671, 829, 1053, 1385, 2225, 3130, 3159
BspQI	GCTCTTC	1 cut(s)	3040
BspT107I	GGYRCC	2 cut(s)	2225, 2263
BspTI	CTTAAG	1 cut(s)	2963
BsrDI	GCAATG	4 cut(s)	511, 980, 1096, 1759
BsrFI	RCCGGY	1 cut(s)	2665
BsrGI	TGTACA	1 cut(s)	1992
BsrI	ACTGG	4 cut(s)	227, 715, 1602, 2881
BssAI	RCCGGY	1 cut(s)	2665
BssECI	CCNNGG	6 cut(s)	313, 479, 1041, 1320, 1434, 3138
BssNAI	GTATAC	2 cut(s)	736, 2540
BssSI	CACGAG	1 cut(s)	656
BssT1I	CCWWGG	2 cut(s)	313, 1320
Bst1107I	GTATAC	2 cut(s)	736, 2540
Bst2BI	CACGAG	1 cut(s)	656
Bst2UI	CCWGG	8 cut(s)	481, 1043, 1436, 2235, 2291, 2774, 2795, 2824
Bst4CI	ACNGT	9 cut(s)	250, 1637, 1803, 1927, 2116, 2203, 2306, 2465, 3059
Bst6I	CTCTTC	3 cut(s)	1272, 1890, 3040
BstAFI	CTTAAG	1 cut(s)	2963
BstAPI	GCANNNNNTGC	1 cut(s)	1141
BstAUI	TGTACA	1 cut(s)	1992
BstC8I	GCNNGC	2 cut(s)	87, 541
BstDEI	CTNAG	9 cut(s)	24, 107, 194, 619, 1032, 1085, 1206, 1808, 2586
BstDSI	CCRYGG	1 cut(s)	313
BstEII	GGTNACC	1 cut(s)	1829
BstENI	CCTNNNNNAGG	1 cut(s)	953
BstF5I	GGATG	9 cut(s)	217, 360, 1962, 2162, 2562, 2623, 2905, 2953, 3184
BstH2I	RGCGCY	1 cut(s)	346
BstHHI	GCGC	2 cut(s)	345, 2482
BstMAI	GTCTC	7 cut(s)	1005, 1249, 1809, 2221, 2259, 2569, 2857
BstMCI	CGRYCG	1 cut(s)	2897
BstMWI	GCNNNNNNNGC	7 cut(s)	549, 1132, 1141, 2291, 2479, 2525, 2687
BstNI	CCWGG	8 cut(s)	481, 1043, 1436, 2235, 2291, 2774, 2795, 2824
BstNSI	RCATGY	1 cut(s)	1913
BstPI	GGTNACC	1 cut(s)	1829
BstSCI	CCNGG	9 cut(s)	479, 1041, 1434, 1640, 2233, 2289, 2772, 2793, 2822
BstSFI	CTRYAG	5 cut(s)	1022, 1346, 2397, 2691, 3198
BstSLI	GKGCMC	2 cut(s)	32, 2937
BstV2I	GAAGAC	2 cut(s)	882, 2331
BstX2I	RGATCY	3 cut(s)	460, 821, 3151
BstYI	RGATCY	3 cut(s)	460, 821, 3151
BstZ17I	GTATAC	2 cut(s)	736, 2540
Bsu15I	ATCGAT	1 cut(s)	3159
Bsu36I	CCTNAGG	1 cut(s)	24
BsuI	GTATCC	1 cut(s)	3045
BsuRI	GGCC	8 cut(s)	30, 85, 260, 714, 742, 2102, 2777, 3137
BsuTUI	ATCGAT	1 cut(s)	3159
BtgI	CCRYGG	1 cut(s)	313
BtsCI	GGATG	9 cut(s)	217, 360, 1962, 2162, 2562, 2623, 2905, 2953, 3184
BtsI	GCAGTG	6 cut(s)	289, 298, 683, 1019, 1380, 1758
Cac8I	GCNNGC	2 cut(s)	87, 541
CaiI	CAGNNNCTG	2 cut(s)	480, 1490
CciI	TCATGA	1 cut(s)	706
CfoI	GCGC	2 cut(s)	345, 2482
Cfr10I	RCCGGY	1 cut(s)	2665
ClaI	ATCGAT	1 cut(s)	3159
CsiI	ACCWGGT	2 cut(s)	2793, 2822
Csp6I	GTAC	3 cut(s)	19, 1993, 2264
CspAI	ACCGGT	1 cut(s)	2665
CviQI	GTAC	3 cut(s)	19, 1993, 2264
DdeI	CTNAG	9 cut(s)	24, 107, 194, 619, 1032, 1085, 1206, 1808, 2586
DraI	TTTAAA	1 cut(s)	699
DraIII	CACNNNGTG	2 cut(s)	815, 2659
DrdI	GACNNNNNNGTC	1 cut(s)	2251
DseDI	GACNNNNNNGTC	1 cut(s)	2251
EaeI	YGGCCR	1 cut(s)	712
Eam1104I	CTCTTC	3 cut(s)	1272, 1890, 3040
EarI	CTCTTC	3 cut(s)	1272, 1890, 3040
Ecl136II	GAGCTC	1 cut(s)	3050
Eco130I	CCWWGG	2 cut(s)	313, 1320
Eco24I	GRGCYC	5 cut(s)	32, 1424, 1721, 2924, 3052
Eco47I	GGWCC	6 cut(s)	650, 1038, 1438, 2033, 2160, 2663
Eco53kI	GAGCTC	1 cut(s)	3050
Eco57I	CTGAAG	6 cut(s)	187, 504, 1809, 1907, 2073, 2510
Eco81I	CCTNAGG	1 cut(s)	24
Eco91I	GGTNACC	1 cut(s)	1829
EcoICRI	GAGCTC	1 cut(s)	3050
EcoNI	CCTNNNNNAGG	1 cut(s)	953
EcoO109I	RGGNCCY	2 cut(s)	28, 29
EcoO65I	GGTNACC	1 cut(s)	1829
EcoRI	GAATTC	2 cut(s)	2738, 2835
EcoRII	CCWGG	8 cut(s)	479, 1041, 1434, 2233, 2289, 2772, 2793, 2822
EcoT14I	CCWWGG	2 cut(s)	313, 1320
EcoT22I	ATGCAT	2 cut(s)	883, 1776
EcoT38I	GRGCYC	5 cut(s)	32, 1424, 1721, 2924, 3052
ErhI	CCWWGG	2 cut(s)	313, 1320
Esp3I	CGTCTC	3 cut(s)	2259, 2569, 2857
FalI	AAGNNNNNCTT	2 cut(s)	1149, 1181
FaqI	GGGAC	5 cut(s)	1024, 1634, 2676, 2802, 2887
FauI	CCCGC	1 cut(s)	2116
FblI	GTMKAC	3 cut(s)	735, 2539, 3061
FokI	GGATG	9 cut(s)	224, 367, 1969, 2169, 2569, 2630, 2912, 2960, 3191
FriOI	GRGCYC	5 cut(s)	32, 1424, 1721, 2924, 3052
FspBI	CTAG	4 cut(s)	159, 489, 1308, 3012
GlaI	GCGC	2 cut(s)	344, 2481
GsuI	CTGGAG	6 cut(s)	244, 933, 1080, 2241, 2256, 2861
HaeII	RGCGCY	1 cut(s)	346
HaeIII	GGCC	8 cut(s)	30, 85, 260, 714, 742, 2102, 2777, 3137
HapII	CCGG	5 cut(s)	585, 1641, 2615, 2666, 2897
HhaI	GCGC	2 cut(s)	345, 2482
Hin6I	GCGC	2 cut(s)	343, 2480
HinP1I	GCGC	2 cut(s)	343, 2480
HincII	GTYRAC	1 cut(s)	1297
HindII	GTYRAC	1 cut(s)	1297
HpaII	CCGG	5 cut(s)	585, 1641, 2615, 2666, 2897
HphI	GGTGA	7 cut(s)	624, 1066, 1458, 1823, 2477, 2812, 3158
Hpy166II	GTNNAC	9 cut(s)	661, 736, 1297, 1799, 1995, 2302, 2540, 2882, 3062
Hpy8I	GTNNAC	9 cut(s)	661, 736, 1297, 1799, 1995, 2302, 2540, 2882, 3062
HpyCH4III	ACNGT	9 cut(s)	250, 1637, 1803, 1927, 2116, 2203, 2306, 2465, 3059
HpyCH4IV	ACGT	2 cut(s)	1786, 2866
HpyF10VI	GCNNNNNNNGC	7 cut(s)	549, 1132, 1141, 2291, 2479, 2525, 2687
HpyF3I	CTNAG	9 cut(s)	24, 107, 194, 619, 1032, 1085, 1206, 1808, 2586
HpySE526I	ACGT	2 cut(s)	1786, 2866
HspAI	GCGC	2 cut(s)	343, 2480
Kpn2I	TCCGGA	1 cut(s)	584
KpnI	GGTACC	1 cut(s)	2267
LguI	GCTCTTC	1 cut(s)	3040
LmnI	GCTCC	5 cut(s)	1099, 1630, 1724, 1947, 2237
MabI	ACCWGGT	2 cut(s)	2793, 2822
MaeI	CTAG	4 cut(s)	159, 489, 1308, 3012
MaeII	ACGT	2 cut(s)	1786, 2866
MfeI	CAATTG	1 cut(s)	2095
MflI	RGATCY	3 cut(s)	460, 821, 3151
MhlI	GDGCHC	9 cut(s)	32, 517, 1114, 1424, 1721, 2242, 2924, 2937, 3052
MlsI	TGGCCA	1 cut(s)	714
MluNI	TGGCCA	1 cut(s)	714
MlyI	GAGTC	5 cut(s)	638, 773, 1481, 2345, 2489
MmeI	TCCRAC	6 cut(s)	1695, 2702, 2812, 2840, 2917, 3156
Mox20I	TGGCCA	1 cut(s)	714
Mph1103I	ATGCAT	2 cut(s)	883, 1776
MroI	TCCGGA	1 cut(s)	584
MroXI	GAANNNNTTC	1 cut(s)	333
MscI	TGGCCA	1 cut(s)	714
MseI	TTAA	9 cut(s)	371, 698, 953, 1190, 1848, 2151, 2358, 2766, 2964
MslI	CAYNNNNRTG	3 cut(s)	565, 1471, 3191
Msp20I	TGGCCA	1 cut(s)	714
MspA1I	CMGCKG	3 cut(s)	458, 1554, 2081
MspCI	CTTAAG	1 cut(s)	2963
MspI	CCGG	5 cut(s)	585, 1641, 2615, 2666, 2897
MspR9I	CCNGG	9 cut(s)	481, 1043, 1436, 1642, 2235, 2291, 2774, 2795, 2824
MunI	CAATTG	1 cut(s)	2095
Mva1269I	GAATGC	3 cut(s)	688, 2180, 2616
MvaI	CCWGG	8 cut(s)	481, 1043, 1436, 2235, 2291, 2774, 2795, 2824
MwoI	GCNNNNNNNGC	7 cut(s)	549, 1132, 1141, 2291, 2479, 2525, 2687
NciI	CCSGG	1 cut(s)	1642
NcoI	CCATGG	1 cut(s)	313
NlaIV	GGNNCC	6 cut(s)	30, 1040, 1720, 2227, 2265, 2664
NmeAIII	GCCGAG	1 cut(s)	3163
NmuCI	GTSAC	4 cut(s)	630, 1829, 2306, 2484
NsiI	ATGCAT	2 cut(s)	883, 1776
NspI	RCATGY	1 cut(s)	1913
OliI	CACNNNNGTG	1 cut(s)	1471
PagI	TCATGA	1 cut(s)	706
PciSI	GCTCTTC	1 cut(s)	3040
PcsI	WCGNNNNNNNCGW	1 cut(s)	2890
PctI	GAATGC	3 cut(s)	688, 2180, 2616
PdmI	GAANNNNTTC	1 cut(s)	333
PfoI	TCCNGGA	1 cut(s)	2233
PinAI	ACCGGT	1 cut(s)	2665
PleI	GAGTC	5 cut(s)	637, 773, 1481, 2344, 2489
PpsI	GAGTC	5 cut(s)	637, 773, 1481, 2344, 2489
PsiI	TTATAA	2 cut(s)	1970, 2010
Psp124BI	GAGCTC	1 cut(s)	3052
Psp6I	CCWGG	8 cut(s)	479, 1041, 1434, 2233, 2289, 2772, 2793, 2822
PspEI	GGTNACC	1 cut(s)	1829
PspGI	CCWGG	8 cut(s)	479, 1041, 1434, 2233, 2289, 2772, 2793, 2822
PspN4I	GGNNCC	6 cut(s)	30, 1040, 1720, 2227, 2265, 2664
PspOMI	GGGCCC	1 cut(s)	28
PstI	CTGCAG	3 cut(s)	1026, 2401, 2695
PstNI	CAGNNNCTG	2 cut(s)	480, 1490
PsuI	RGATCY	3 cut(s)	460, 821, 3151
PvuII	CAGCTG	2 cut(s)	1554, 2081
RsaI	GTAC	3 cut(s)	20, 1994, 2265
RsaNI	GTAC	3 cut(s)	19, 1993, 2264
RseI	CAYNNNNRTG	3 cut(s)	565, 1471, 3191
SacI	GAGCTC	1 cut(s)	3052
SapI	GCTCTTC	1 cut(s)	3040
SaqAI	TTAA	9 cut(s)	371, 698, 953, 1190, 1848, 2151, 2358, 2766, 2964
SchI	GAGTC	5 cut(s)	638, 773, 1481, 2345, 2489
ScrFI	CCNGG	9 cut(s)	481, 1043, 1436, 1642, 2235, 2291, 2774, 2795, 2824
SduI	GDGCHC	9 cut(s)	32, 517, 1114, 1424, 1721, 2242, 2924, 2937, 3052
SexAI	ACCWGGT	2 cut(s)	2793, 2822
SfcI	CTRYAG	5 cut(s)	1022, 1346, 2397, 2691, 3198
SinI	GGWCC	6 cut(s)	650, 1038, 1438, 2033, 2160, 2663
SmiMI	CAYNNNNRTG	3 cut(s)	565, 1471, 3191
SmlI	CTYRAG	3 cut(s)	359, 1790, 2963
SmoI	CTYRAG	3 cut(s)	359, 1790, 2963
SsiI	CCGC	7 cut(s)	12, 82, 162, 458, 668, 2123, 2163
SspI	AATATT	1 cut(s)	837
SspMI	CTAG	4 cut(s)	159, 489, 1308, 3012
SstI	GAGCTC	1 cut(s)	3052
StyD4I	CCNGG	9 cut(s)	479, 1041, 1434, 1640, 2233, 2289, 2772, 2793, 2822
StyI	CCWWGG	2 cut(s)	313, 1320
TaaI	ACNGT	9 cut(s)	250, 1637, 1803, 1927, 2116, 2203, 2306, 2465, 3059
TaiI	ACGT	2 cut(s)	1789, 2869
TaqI	TCGA	7 cut(s)	34, 1273, 1704, 1858, 3078, 3117, 3159
TaqII	GACCGA	1 cut(s)	2261
TatI	WGTACW	1 cut(s)	1992
TauI	GCSGC	1 cut(s)	85
Tru1I	TTAA	9 cut(s)	371, 698, 953, 1190, 1848, 2151, 2358, 2766, 2964
Tru9I	TTAA	9 cut(s)	371, 698, 953, 1190, 1848, 2151, 2358, 2766, 2964
TseFI	GTSAC	4 cut(s)	630, 1829, 2306, 2484
Tsp45I	GTSAC	4 cut(s)	630, 1829, 2306, 2484
TspDTI	ATGAA	9 cut(s)	342, 380, 716, 1362, 1965, 1994, 2159, 2387, 3181
TspGWI	ACGGA	2 cut(s)	2455, 3077
Vha464I	CTTAAG	1 cut(s)	2963
VpaK11BI	GGWCC	6 cut(s)	650, 1038, 1438, 2033, 2160, 2663
XagI	CCTNNNNNAGG	1 cut(s)	953
XapI	RAATTY	6 cut(s)	102, 746, 1057, 2316, 2738, 2835
XceI	RCATGY	1 cut(s)	1913
XmiI	GTMKAC	3 cut(s)	735, 2539, 3061
XmnI	GAANNNNTTC	1 cut(s)	333
XspI	CTAG	4 cut(s)	159, 489, 1308, 3012
Zsp2I	ATGCAT	2 cut(s)	883, 1776

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.