MD16G1139000.v1.1

calmodulin-binding transcription activator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0001204
Physical Location & Seq
Forward (+)
10715209 .. 10720297
5089 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1139000.v1.1.491

Sequence Viewer

Length: 1074 bp
ATGAAATCTAGGGTTTATGCGAGCTCCGTCATTTGGTCGCCCATGGCTGATACCAGGAGATGCCTCCCCAATCAACACCTAGACCTTTCCCAGATATTGCAAGAATCAAAGAACCGTGGGCTTCGTCCAGCTGAAATTTGTGAAATGCTTCGCAACTATCAGAATTTTGAGTTGACCACAGATCCGCCCGTCAGGCCTCCAGGTGGTTCTTTGTACCTGTTTGATCGAAAAGCACTTAGATATTTTCGTAAAGACGGTCATCGCTGGAGGAAGAAAAAAGATGGAAAGACTGTAAAAGAAGCCCATGAAAAGTTGAAGGCTGGTAGTGTAGACGTACTTCAATGTTACTATGCCCATGGGGAGGACTATGCGAATTTTCAACGGCGGAGTTATTGGATGCTTGACACGCATTTACAGCACATCGTTCTTGTGCATTACAGAAATGTGGAAGAAGCAAATTCCGCTGCCCGTATGTTTCAAACATCCTTTGATTCAAGTCCAGTTCAAACATCCTTTGCTTCAAGTCCAATTCAAACATCCTTTGCTTCAAGTCCAGTTCGAACATCCTTTGCTTCAAGTCCAAACAGAGTTGACTTGAGTGGACAAACATTATCGACAGAATTTGAGGATGTGGGTTCTCGGAGAGATGCAGGAATTTCATCTATTGAGCAACCGATGCTTGGTTCCATTTTTCATAATGCTTCTCTGCGTCCACAAAAAATTGGAGGATTTCCTGAGTCATTCAGGGATCCCTCTAGTACGTGGTATGGTGGACCTAAATTTGATCATGGTGATGGATTATCTGTCTGGCACGGAATGGATAGCTCAACGAGAAATGAGCACAGCATGCATGCCCAAAACTTTTTTGTTGAAGAACCTAACGGAGTTGACTTGATAACTAATAAGCTAACCAATTCTAGAGTGGATGTTCGTAGTGGAGTCAATGATGTAACTTGCGAAGATAGGTTGACCACTAACATAGATGTCCAAATTGTCTCAGCACCTTCTCGAAGAGAAGATCAGGTGCCAAGGGAGCATGTCTTCGATGTGCTTCATCCTCAGGTTCAAGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000981 GO:0000982 GO:0001077 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005516 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0006950 GO:0006952 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009266 GO:0009409 GO:0009605 GO:0009607 GO:0009617 GO:0009620 GO:0009628 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010150 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031347 GO:0031349 GO:0032501 GO:0032502 GO:0032774 GO:0033554 GO:0034641 GO:0034645 GO:0034654 GO:0042742 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048366 GO:0048367 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0048731 GO:0048827 GO:0048856 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070417 GO:0071704 GO:0080090 GO:0080134 GO:0090304 GO:0090693 GO:0097159 GO:0097659 GO:0098542 GO:0099402 GO:0140110 GO:1900367 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:2000068 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 CG-1 IQ TIG

Protein Analysis

358

Amino Acids

40.57

Weight (kDa)

6.93

Isoelectric Point (pI)

60.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CG-1 PF03859 36 - 148 6.9e-46 CG-1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1024
AccI GTMKAC 1 cut(s) 330
AciI CCGC 3 cut(s) 185, 385, 462
AclWI GGATC 3 cut(s) 176, 743, 756
AcsI RAATTY 7 cut(s) 135, 163, 373, 457, 620, 654, 779
AfaI GTAC 3 cut(s) 215, 336, 760
AfiI CCNNNNNNNGG 4 cut(s) 33, 203, 361, 680
AjnI CCWGG 2 cut(s) 53, 199
AluBI AGCT 4 cut(s) 24, 131, 825, 907
AluI AGCT 4 cut(s) 24, 131, 825, 907
Alw21I GWGCWC 2 cut(s) 26, 843
Alw26I GTCTC 1 cut(s) 1000
AlwI GGATC 3 cut(s) 176, 743, 756
AoxI GGCC 1 cut(s) 194
ApeKI GCWGC 1 cut(s) 464
ApoI RAATTY 7 cut(s) 135, 163, 373, 457, 620, 654, 779
Asp700I GAANNNNTTC 1 cut(s) 147
AspS9I GGNCC 1 cut(s) 773
AsuHPI GGTGA 1 cut(s) 803
AsuII TTCGAA 1 cut(s) 559
AvaII GGWCC 1 cut(s) 773
AxyI CCTNAGG 1 cut(s) 1059
BamHI GGATCC 1 cut(s) 748
BanI GGYRCC 1 cut(s) 1024
BanII GRGCYC 1 cut(s) 26
BarI GAAGNNNNNNTAC 2 cut(s) 321, 353
BbsI GAAGAC 1 cut(s) 1033
Bbv12I GWGCWC 2 cut(s) 26, 843
BbvI GCAGC 1 cut(s) 451
BccI CCATC 2 cut(s) 275, 788
BceAI ACGGC 1 cut(s) 398
BciT130I CCWGG 2 cut(s) 55, 201
BclI TGATCA 1 cut(s) 784
BcoDI GTCTC 1 cut(s) 1000
BfaI CTAG 4 cut(s) 9, 80, 756, 918
BglI GCCNNNNNGGC 1 cut(s) 193
BisI GCNGC 1 cut(s) 465
BlsI GCNGC 1 cut(s) 466
Bme1390I CCNGG 2 cut(s) 55, 201
Bme18I GGWCC 1 cut(s) 773
BmgT120I GGNCC 1 cut(s) 773
BmiI GGNNCC 3 cut(s) 685, 750, 1026
BmrFI CCNGG 2 cut(s) 55, 201
BmsI GCATC 4 cut(s) 50, 387, 637, 666
BpiI GAAGAC 1 cut(s) 1033
BpmI CTGGAG 2 cut(s) 183, 286
Bpu14I TTCGAA 1 cut(s) 559
BpuEI CTTGAG 1 cut(s) 616
BsaAI YACGTR 1 cut(s) 762
BsaJI CCNNGG 4 cut(s) 42, 115, 355, 1028
Bsc4I CCNNNNNNNGG 4 cut(s) 33, 203, 361, 680
Bse1I ACTGG 2 cut(s) 500, 554
Bse21I CCTNAGG 1 cut(s) 1059
BseBI CCWGG 2 cut(s) 55, 201
BseDI CCNNGG 4 cut(s) 42, 115, 355, 1028
BseGI GGATG 8 cut(s) 402, 482, 509, 536, 563, 634, 931, 1054
BseLI CCNNNNNNNGG 4 cut(s) 33, 203, 361, 680
BseMII CTCAG 3 cut(s) 726, 1011, 1073
BseNI ACTGG 2 cut(s) 500, 554
BseXI GCAGC 1 cut(s) 451
BshFI GGCC 1 cut(s) 196
BshNI GGYRCC 1 cut(s) 1024
BsiHKAI GWGCWC 2 cut(s) 26, 843
BslI CCNNNNNNNGG 4 cut(s) 33, 203, 361, 680
BsmAI GTCTC 1 cut(s) 1000
BsnI GGCC 1 cut(s) 196
Bsp119I TTCGAA 1 cut(s) 559
Bsp1286I GDGCHC 2 cut(s) 26, 843
Bsp143I GATC 5 cut(s) 181, 223, 748, 784, 1018
Bsp19I CCATGG 2 cut(s) 42, 355
BspACI CCGC 3 cut(s) 185, 385, 462
BspANI GGCC 1 cut(s) 196
BspCNI CTCAG 3 cut(s) 727, 1010, 1072
BspLI GGNNCC 3 cut(s) 685, 750, 1026
BspPI GGATC 3 cut(s) 176, 743, 756
BspT104I TTCGAA 1 cut(s) 559
BspT107I GGYRCC 1 cut(s) 1024
BsrI ACTGG 2 cut(s) 500, 554
BssECI CCNNGG 4 cut(s) 42, 115, 355, 1028
BssMI GATC 5 cut(s) 181, 223, 748, 784, 1018
BssT1I CCWWGG 3 cut(s) 42, 355, 1028
Bst2UI CCWGG 2 cut(s) 55, 201
Bst4CI ACNGT 3 cut(s) 116, 257, 292
Bst6I CTCTTC 1 cut(s) 1006
BstAPI GCANNNNNTGC 2 cut(s) 676, 847
BstBAI YACGTR 1 cut(s) 762
BstBI TTCGAA 1 cut(s) 559
BstC8I GCNNGC 3 cut(s) 22, 848, 852
BstDEI CTNAG 4 cut(s) 236, 735, 997, 1059
BstDSI CCRYGG 3 cut(s) 42, 115, 355
BstF5I GGATG 8 cut(s) 402, 482, 509, 536, 563, 634, 931, 1054
BstKTI GATC 5 cut(s) 184, 226, 751, 787, 1021
BstMAI GTCTC 1 cut(s) 1000
BstMBI GATC 5 cut(s) 181, 223, 748, 784, 1018
BstMWI GCNNNNNNNGC 7 cut(s) 193, 406, 415, 461, 676, 847, 1033
BstNI CCWGG 2 cut(s) 55, 201
BstNSI RCATGY 3 cut(s) 850, 854, 1040
BstSCI CCNGG 2 cut(s) 53, 199
BstV1I GCAGC 1 cut(s) 451
BstV2I GAAGAC 1 cut(s) 1033
BstX2I RGATCY 2 cut(s) 181, 748
BstYI RGATCY 2 cut(s) 181, 748
Bsu36I CCTNAGG 1 cut(s) 1059
BsuRI GGCC 1 cut(s) 196
BtgI CCRYGG 3 cut(s) 42, 115, 355
BtgZI GCGATG 1 cut(s) 245
BtsCI GGATG 8 cut(s) 402, 482, 509, 536, 563, 634, 931, 1054
Cac8I GCNNGC 3 cut(s) 22, 848, 852
Cfr13I GGNCC 1 cut(s) 773
CseI GACGC 1 cut(s) 698
Csp6I GTAC 3 cut(s) 214, 335, 759
CviAII CATG 7 cut(s) 43, 305, 356, 788, 847, 851, 1037
CviJI RGCY 9 cut(s) 24, 47, 121, 131, 196, 302, 320, 825, 907
CviKI_1 RGCY 9 cut(s) 24, 47, 121, 131, 196, 302, 320, 825, 907
CviQI GTAC 3 cut(s) 214, 335, 759
DdeI CTNAG 4 cut(s) 236, 735, 997, 1059
DpnI GATC 5 cut(s) 183, 225, 750, 786, 1020
DpnII GATC 5 cut(s) 181, 223, 748, 784, 1018
Eam1104I CTCTTC 1 cut(s) 1006
EarI CTCTTC 1 cut(s) 1006
EciI GGCGGA 2 cut(s) 174, 400
Ecl136II GAGCTC 1 cut(s) 24
Eco130I CCWWGG 3 cut(s) 42, 355, 1028
Eco147I AGGCCT 1 cut(s) 196
Eco24I GRGCYC 1 cut(s) 26
Eco47I GGWCC 1 cut(s) 773
Eco53kI GAGCTC 1 cut(s) 24
Eco81I CCTNAGG 1 cut(s) 1059
EcoICRI GAGCTC 1 cut(s) 24
EcoRII CCWGG 2 cut(s) 53, 199
EcoT14I CCWWGG 3 cut(s) 42, 355, 1028
EcoT22I ATGCAT 1 cut(s) 852
EcoT38I GRGCYC 1 cut(s) 26
ErhI CCWWGG 3 cut(s) 42, 355, 1028
FaeI CATG 7 cut(s) 46, 308, 359, 791, 850, 854, 1040
FatI CATG 7 cut(s) 42, 304, 355, 787, 846, 850, 1036
FbaI TGATCA 1 cut(s) 784
FblI GTMKAC 1 cut(s) 330
Fnu4HI GCNGC 1 cut(s) 465
FokI GGATG 8 cut(s) 409, 469, 496, 523, 550, 641, 938, 1041
FriOI GRGCYC 1 cut(s) 26
Fsp4HI GCNGC 1 cut(s) 465
FspBI CTAG 4 cut(s) 9, 80, 756, 918
GluI GCNGC 1 cut(s) 465
GsuI CTGGAG 2 cut(s) 183, 286
HaeIII GGCC 1 cut(s) 196
HgaI GACGC 1 cut(s) 698
Hin1II CATG 7 cut(s) 46, 308, 359, 791, 850, 854, 1040
HincII GTYRAC 4 cut(s) 174, 592, 889, 969
HindII GTYRAC 4 cut(s) 174, 592, 889, 969
HinfI GANTC 4 cut(s) 104, 491, 737, 939
HphI GGTGA 1 cut(s) 803
Hpy166II GTNNAC 8 cut(s) 174, 331, 592, 602, 713, 773, 889, 969
Hpy188I TCNGA 2 cut(s) 162, 642
Hpy188III TCNNGA 4 cut(s) 734, 918, 1008, 1067
Hpy8I GTNNAC 8 cut(s) 174, 331, 592, 602, 713, 773, 889, 969
HpyAV CCTTC 2 cut(s) 310, 1014
HpyCH4III ACNGT 3 cut(s) 116, 257, 292
HpyCH4IV ACGT 2 cut(s) 333, 761
HpyCH4V TGCA 4 cut(s) 100, 433, 650, 850
HpyF10VI GCNNNNNNNGC 7 cut(s) 193, 406, 415, 461, 676, 847, 1033
HpyF3I CTNAG 4 cut(s) 236, 735, 997, 1059
HpySE526I ACGT 2 cut(s) 333, 761
Hsp92II CATG 7 cut(s) 46, 308, 359, 791, 850, 854, 1040
Ksp22I TGATCA 1 cut(s) 784
Kzo9I GATC 5 cut(s) 181, 223, 748, 784, 1018
LmnI GCTCC 2 cut(s) 29, 1033
Lsp1109I GCAGC 1 cut(s) 451
LweI GCATC 4 cut(s) 50, 387, 637, 666
MaeI CTAG 4 cut(s) 9, 80, 756, 918
MaeII ACGT 2 cut(s) 333, 761
MaeIII GTNAC 2 cut(s) 344, 949
MalI GATC 5 cut(s) 183, 225, 750, 786, 1020
MboI GATC 5 cut(s) 181, 223, 748, 784, 1018
MboII GAAGA 7 cut(s) 283, 461, 884, 971, 1023, 1028, 1033
MflI RGATCY 2 cut(s) 181, 748
MhlI GDGCHC 2 cut(s) 26, 843
MlyI GAGTC 2 cut(s) 746, 948
MnlI CCTC 8 cut(s) 74, 207, 261, 355, 619, 719, 763, 1068
Mph1103I ATGCAT 1 cut(s) 852
MroXI GAANNNNTTC 1 cut(s) 147
MslI CAYNNNNRTG 1 cut(s) 792
MspA1I CMGCKG 2 cut(s) 131, 464
MspR9I CCNGG 2 cut(s) 55, 201
MvaI CCWGG 2 cut(s) 55, 201
MwoI GCNNNNNNNGC 7 cut(s) 193, 406, 415, 461, 676, 847, 1033
NcoI CCATGG 2 cut(s) 42, 355
NdeII GATC 5 cut(s) 181, 223, 748, 784, 1018
NlaIII CATG 7 cut(s) 46, 308, 359, 791, 850, 854, 1040
NlaIV GGNNCC 3 cut(s) 685, 750, 1026
NsiI ATGCAT 1 cut(s) 852
NspI RCATGY 3 cut(s) 850, 854, 1040
NspV TTCGAA 1 cut(s) 559
PaeI GCATGC 2 cut(s) 850, 854
PceI AGGCCT 1 cut(s) 196
PdmI GAANNNNTTC 1 cut(s) 147
PfeI GAWTC 2 cut(s) 104, 491
PkrI GCNGC 1 cut(s) 466
PleI GAGTC 2 cut(s) 745, 947
PpsI GAGTC 2 cut(s) 745, 947
Ppu21I YACGTR 1 cut(s) 762
Psp124BI GAGCTC 1 cut(s) 26
Psp6I CCWGG 2 cut(s) 53, 199
PspGI CCWGG 2 cut(s) 53, 199
PspN4I GGNNCC 3 cut(s) 685, 750, 1026
PspPI GGNCC 1 cut(s) 773
PsuI RGATCY 2 cut(s) 181, 748
PvuII CAGCTG 1 cut(s) 131
RsaI GTAC 3 cut(s) 215, 336, 760
RsaNI GTAC 3 cut(s) 214, 335, 759
RseI CAYNNNNRTG 1 cut(s) 792
SacI GAGCTC 1 cut(s) 26
SatI GCNGC 1 cut(s) 465
Sau3AI GATC 5 cut(s) 181, 223, 748, 784, 1018
Sau96I GGNCC 1 cut(s) 773
SchI GAGTC 2 cut(s) 746, 948
ScrFI CCNGG 2 cut(s) 55, 201
SduI GDGCHC 2 cut(s) 26, 843
SfaNI GCATC 4 cut(s) 50, 387, 637, 666
SfuI TTCGAA 1 cut(s) 559
SinI GGWCC 1 cut(s) 773
SmiMI CAYNNNNRTG 1 cut(s) 792
SmlI CTYRAG 1 cut(s) 595
SmoI CTYRAG 1 cut(s) 595
SphI GCATGC 2 cut(s) 850, 854
SseBI AGGCCT 1 cut(s) 196
SsiI CCGC 3 cut(s) 185, 385, 462
SspMI CTAG 4 cut(s) 9, 80, 756, 918
SstI GAGCTC 1 cut(s) 26
StuI AGGCCT 1 cut(s) 196
StyD4I CCNGG 2 cut(s) 53, 199
StyI CCWWGG 3 cut(s) 42, 355, 1028
TaaI ACNGT 3 cut(s) 116, 257, 292
TaiI ACGT 2 cut(s) 336, 764
TaqI TCGA 5 cut(s) 226, 559, 614, 1009, 1044
TfiI GAWTC 2 cut(s) 104, 491
TseI GCWGC 1 cut(s) 464
TspDTI ATGAA 5 cut(s) 17, 321, 648, 683, 1043
TspGWI ACGGA 3 cut(s) 16, 828, 897
VpaK11BI GGWCC 1 cut(s) 773
XapI RAATTY 7 cut(s) 135, 163, 373, 457, 620, 654, 779
XbaI TCTAGA 1 cut(s) 917
XceI RCATGY 3 cut(s) 850, 854, 1040
XcmI CCANNNNNNNNNTGG 1 cut(s) 919
XmiI GTMKAC 1 cut(s) 330
XmnI GAANNNNTTC 1 cut(s) 147
XspI CTAG 4 cut(s) 9, 80, 756, 918
Zsp2I ATGCAT 1 cut(s) 852
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.